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Results for “centrifuge method”

Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 127 records · Page 7
All sourcesNASA NTRSDOE OSTI
NASA NTRS2000

The density of apical cells of dark-grown protonemata of the moss Ceratodon purpureus

Determinations of plant or algal cell density (cell mass divided by volume) have rarely accounted for the extracellular matrix or shrinkage during isolation. Three techniques were used to indirectly estimate the density of intact apical cells from protonemata of the moss Ceratodon purpureus. First, the volume fraction of each cell component was determined by stereology, and published values for component density were used to extrapolate to the entire cell. Second, protonemal tips were immersed in bovine serum albumin solutions of different densities, and then the equilibrium density was corrected for the mass of the cell wall. Third, apical cell protoplasts were centrifuged in low-osmolarity gradients, and values were corrected for shrinkage during protoplast isolation. Values from centrifugation (1.004 to 1.015 g/cm3) were considerably lower than from other methods (1.046 to 1.085 g/cm3). This work appears to provide the first corrected estimates of the density of any plant cell. It also documents a method for the isolation of protoplasts specifically from apical cells of protonemal filaments.

NASA Discipline Plant Biology↗
NASA NTRS1974

Selection of appropriate test method for simulating steady-state spacecraft launch loads

In ground testing prior to launch the static loads portion of the launch profile may be simulated by either applying static loads directly to the spacecraft through an appropriate loading facility or by subjecting the spacecraft to acceleration loads on a centrifuge. The factors to be considered in selecting one of the two simulation methods are discussed, giving attention to the relative advantages of the two methods in each case.

Shockey, E. F.↗
NASA NTRS2007

Liquid Metering Centrifuge Sticks (LMCS): A Centrifugal Approach to Metering Known Sample Volumes for Colorimetric Solid Phase Extraction (C-SPE)

Phase separation is one of the most significant obstacles encountered during the development of analytical methods for water quality monitoring in spacecraft environments. Removing air bubbles from water samples prior to analysis is a routine task on earth; however, in the absence of gravity, this routine task becomes extremely difficult. This paper details the development and initial ground testing of liquid metering centrifuge sticks (LMCS), devices designed to collect and meter a known volume of bubble-free water in microgravity. The LMCS uses centrifugal force to eliminate entrapped air and reproducibly meter liquid sample volumes for analysis with Colorimetric Solid Phase Extraction (C-SPE). C-SPE is a sorption-spectrophotometric platform that is being developed as a potential spacecraft water quality monitoring system. C-SPE utilizes solid phase extraction membranes impregnated with analyte-specific colorimetric reagents to concentrate and complex target analytes in spacecraft water samples. The mass of analyte extracted from the water sample is determined using diffuse reflectance (DR) data collected from the membrane surface and an analyte-specific calibration curve. The analyte concentration can then be calculated from the mass of extracted analyte and the volume of the sample analyzed. Previous flight experiments conducted in microgravity conditions aboard the NASA KC-135 aircraft demonstrated that the inability to collect and meter a known volume of water using a syringe was a limiting factor in the accuracy of C-SPE measurements. Herein, results obtained from ground based C-SPE experiments using ionic silver as a test analyte and either the LMCS or syringes for sample metering are compared to evaluate the performance of the LMCS. These results indicate very good agreement between the two sample metering methods and clearly illustrate the potential of utilizing centrifugal forces to achieve phase separation and metering of water samples in microgravity.

Gazda, Daniel B.↗
DOE OSTI2021

Probing Clustering Dynamics between Silicon and PAA or LiPAA Slurries under Processing Conditions

This work explores the complex interplay between slurry aggregation, agglomeration, and conformation (i.e., shape) of poly(acrylic acid) (PAA)- and lithiated PAA-based silicon slurries as a function of the shear rate and the resulting slurry homogeneity. These values were measured by small-angle neutron scattering (SANS) and rheology-coupled ultra-SANS at conditions relevant to battery electrode casting. Different binder solution preparation methods, either a ball milling (BM) process or a planetary centrifugal mixing (PCM) process, dramatically modify the resulting polymer dynamics and organization around a silicon material. This is due to the different energy profiles of mixing where the more violent and higher energy PCM causes extensive breakdown and reformation of the binder, which is now likely in a branched conformation, while the lower energy BM results in simply lower-molecular weight linear polymers. In conclusion, the breakdown and reorganization of the polymer structure affect silicon slurry homogeneity, which affects subsequent electrode architecture.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
NASA NTRS1977

Survey and Experimental Testing of Nongravimetric Mass Measurement Devices

Documentation presented describes the design, testing, and evaluation of an accelerated gravimetric balance, a low mass air bearing oscillator of the spring-mass type, and a centrifugal device for liquid mass measurement. A direct mass readout method was developed to replace the oscillation period readout method which required manual calculations to determine mass. A protoype 25 gram capacity micro mass measurement device was developed and tested.

Oakey, W. E.↗
NASA NTRS1978

Stress analysis study in cooled radial inflow turbine

With increased turbine inlet temperatures, numerical methods of thermal and stress analysis are becoming more valuable in the design of air-cooled turbines. This paper presents a study of the stresses associated with different cooling patterns in a radial inflow turbine rotor. The finite element method is used in the stress calculations taking into consideration centrifugal, thermal and aerodynamic loading. The effects of temperature distribution and the presence of internal cooling passages are discussed.

Hamed, A.↗
NASA NTRS1985

Development of an explicit multigrid algorithm for quasi-three-dimensional viscous flows in turbo-machinery

A rapid quasi three-dimensional analysis was developed for blade-to-blade flows in turbomachinery. The analysis solves the unsteady Euler or thin layer Navier-Stokes equations in a body-fitted coordinate system. It accounts for the effects of rotation, radius change, and stream-surface thickness. The Baldwin-Lomax eddy-viscosity model is used for turbulent flows. The equations which are solved by a two-stage Runge-Kutta scheme made efficient by use of vectorization, a variable time-step, and a flux-based multigrid scheme, are described. A stability analysis is presented for the two-stage scheme. Results for a flat-plate model problem show the applicability of the method to axial, radial, and rotating geometries. Results for a centrifugal impeller and a radial diffuser show that the quasi three-dimensional viscous analysis can be a practical design tool.

Chima, R. V.↗
NASA NTRS1986

Development of an explicit multigrid algorithm for quasi-three-dimensional viscous flows in turbomachinery

A rapid quasi three-dimensional analysis was developed for blade-to-blade flows in turbomachinery. The analysis solves the unsteady Euler or thin layer Navier-Stokes equations in a body-fitted coordinate system. It accounts for the effects of rotation, radius change, and stream-surface thickness. The Baldwin-Lomax eddy-viscosity model is used for turbulent flows. The equations which are solved b a two-stage Runge-Kutta scheme made efficient by use of vectorization, a variable time-step, and a flux-based multigrid scheme, are described. A stability analysis is presented for the two-stage. Results for a flat-plate model problem show the applicability of the method to axial, radial, and rotating geometries. Results for a centrifugal impeller and a radial diffuser show that the quasi three-dimensional viscous analysis can be a practical design tool.

Chima, R. V.↗
NASA NTRS1995

Centrifugal and Axial Pump Design and Off-Design Performance Prediction

A meanline pump-flow modeling method has been developed to provide a fast capability for modeling pumps of cryogenic rocket engines. Based on this method, a meanline pump-flow code PUMPA was written that can predict the performance of pumps at off-design operating conditions, given the loss of the diffusion system at the design point. The design-point rotor efficiency and slip factors are obtained from empirical correlations to rotor-specific speed and geometry. The pump code can model axial, inducer, mixed-flow, and centrifugal pumps and can model multistage pumps in series. The rapid input setup and computer run time for this meanline pump flow code make it an effective analysis and conceptual design tool. The map-generation capabilities of the code provide the information needed for interfacing with a rocket engine system modeling code. The off-design and multistage modeling capabilities of PUMPA permit the user to do parametric design space exploration of candidate pump configurations and to provide head-flow maps for engine system evaluation.

Veres, Joseph P.↗
NASA NTRS2002

Three-Dimensional Postbuckling Analysis of Curved Beams

Presented here is a method of solving highly flexible curved beam undergoing huge static or quasi-static deformations. A geometrically exact beam theory based on the use of Jaumann stresses and strains and exact coordinate transformation is presented in terms of 17 first-order ordinary differential equations, and a multiple shooting method is used to solve the corresponding nonlinear two-point boundary value problems. The geometrically exact beam theory accounts far large rotations, large displacements, initial curvatures, extensionality, and transverse shear strains. Four examples are used to demonstrate this method, including a rotating clamped-free beam under the influence of gravity and centrifugal forces, an L-frame subjected to an in-plane tip load, a circular arch subjected to a concentrated load, and a clamped-hinged helical spring subjected to an axial displacement. Results show that the combination of the multiple shooting method and the geometrically exact beam theory works very well. Moreover, the obtained numerically exact solutions can be used to verify the accuracy of nonlinear finite element codes for nonlinear analysis of complex structures.

Pai, P. Frank↗
DOE OSTI2020

Centrifugal Generation of Droplet-Based 3D Cell Cultures

Quickly and easily producing uniform populations of microsphere-based 3D cell cultures using droplet-based templating methods has the potential to enable widespread use of such platforms in drug discovery or cancer research. Here, we advance the design of centrifuge-based droplet generation devices, describe the use of this platform for droplet generation with controlled cell occupancy, and demonstrate weeklong culture duration. Using simple-to-construct devices and easily implemented protocols, the initial concentration of encapsulated cells is adjustable up to hundreds of cells per microsphere. This work demonstrates the first instance of using centrifugal droplet-generating devices to produce large numbers of cell-encapsulating microspheres. Applications of this versatile methodology include the rapid formation of templated 3D cell culture populations suitable for suspension culture or large batch bioreactor studies that require uniform populations.

Biochemistry & Molecular Biology↗
DOE OSTI2024

Studying microbially induced corrosion on glass using ToF-SIMS

Microbially induced corrosion (MIC) is an emerging topic that has huge environmental impacts, such as long-term evaluation of microbial interactions with radioactive waste glass, environmental cleanup and disposal of radioactive material, and weathering effects of microbes. Time-of-flight secondary ion mass spectrometry (ToF-SIMS), a powerful mass spectral imaging technique with high surface sensitivity, mass resolution, and mass accuracy, can be used to study biofilm effects on different substrates. Understanding how to prepare biofilms on MIC susceptible substrates is critical for proper analysis via ToF-SIMS. We present here a step-by-step protocol for preparing bacterial biofilms for ToF-SIMS analysis, comparing three biofilm preparation techniques: no desalination, centrifugal spinning (CS), and water submersion (WS). Comparisons of two desalinating methods, CS and WS, show a decrease in the media peaks up to 99% using CS and 55% using WS, respectively. Proper desalination methods also can increase biological signals by over four times for fatty acids using WS, for example. ToF-SIMS spectral results show chemical compositional changes of the glass exposed in a Paenibacillus polymyxa SCE2 biofilm, indicating its capability to probe microbiologically induced corrosion of solid surfaces. This represents the proper desalination technique to use without significantly altering biofilm structure and substrate for ToF-SIMS analysis. ToF-SIMS spectral results showed chemical compositional changes of the glass exposed by a Paenibacillus bacterial biofilm over 3-month inoculation. Finally, possible MIC products include various phosphate phase molecules not observed in any control samples with the highest percent increases when experimental samples were compared with biofilm control samples.

36 MATERIALS SCIENCE↗
DOE OSTI2025

Fractionation of Filamentous Algae from Mixed Biofilms

Filamentous algae, which grow in long, hair-like filaments within biofilms, play a crucial role in wastewater treatment due to their ability to produce significant biomass and their resistance to predation compared to traditional microalgal treatments. These algae can effectively uptake and utilize pollutants, particularly excessive nitrogen (ammonia, nitrate, nitrite) and phosphorus (phosphate), making filamentous algae valuable for wastewater treatment, as well as bioethanol and biodiesel production due to high lipid productions. However, each algal species possesses different capacities, necessitating a thorough genetic identification and understanding of each community. A major challenge in accurately assessing these communities is the lack of coverage in large sequencing databases which can lead to misrepresentation of the true composition and abundance of organisms and overall sequencing bias. To address this, I evaluated chemical and physical techniques for separating filamentous algae from mixed biofilms to achieve clean genetic sequencing results. I employed pH washing (0.001M HCl, 0.001M HCl, DiH2O, 0.0001M HCl, 0.001M HCl) for chemical treatment, followed by physical separation through centrifugation (5000rpm, 6500rpm) or filtration (2mm, 250um, 75um). The most successful method was deionized water washing, which yielded clear differences across stacked filters; the 2mm filtrate showed high levels of filamentous algae, with microalgae eluting in the 75um filtrate or remaining within agglutinations of algae larger filters. Base washing eluted the highest concentrations of microalgae, with larger filter sizes retaining more filamentous algae, indicating the breakdown of extracellular polymeric substances (EPS). Our downstream plans include sending the high-throughput next-generation sequencing to confirm the purity and ratios of filamentous and non-filamentous algae, as well as bacteria present, thereby validating the success of our treatments. Potential applications include creating community-based fractions for analysis, refining current sequencing data with clearer isolations, and generating designer biofilms to enhance our understanding of community interactions.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2025

A Model Based Approach to Extract Health Information from Textual Data

In current nuclear power plants (NPPs) a large amount of condition-based data is being generated and stored to assess and monitor component health and performance. The format of this data can be either numeric (e.g., pump vibration data) or textual (e.g., condition report which assess component health). While assessing component health from numeric data can be performed with a large variety of methods, the extraction of information from textual data still remains a challenge. Natural language processing (NLP) methods are starting to be deployed in current NPPs mainly to filter out incident reports (IRs) that are not safety related by employing supervised machine learning methods. However, these methods do not really provide the quantitative information that might be contained in IRs. This paper presents an approach to extract information from textual data (e.g., from IRs, maintenance reports) that is based on NLP data analytics methods coupled with model-based system engineer (MBSE) models. NLP methods are employed to perform syntactic and semantic analyses. Syntactic analysis analyzes the grammatical structure of a sentence; such analysis includes: part of speech (POS) tagging (i.e., identification of grammatic elements of each string - e.g., nouns, verbs), named entity recognition (i.e., identification of text entities - e.g., names, dates, events), and relation extraction (e.g., coreference resolution). On the other hand, semantic analysis is designed to analyze the logic structure of a sentence. Through a specific set of rules, our methods can identify whether a sentence contains health information of a component (e.g., degraded performance, anomaly behavior) or the causal relationship between two events (i.e., a cause-effect pair). An innovative element of our approach is that semantic analysis relies on MBSE models to identify links between textual elements. MBSE are diagrams designed to represent system and component dependencies (from both a form and functional point of view). In our approach, MBSE models emulate system engineer knowledge about component/system architecture. This paper presents in detail how the integration of NLP methods and MBSE models is performed. Few analysis examples focusing on centrifugal pumps are presented.

97 - MATHEMATICS AND COMPUTING↗
NASA NTRS1977

Application of firefly luciferase assay for adenosine triphosphate (ATP) to antimicrobial drug sensitivity testing

The development of a rapid method for determining microbial susceptibilities to antibiotics using the firefly luciferase assay for adenosine triphosphate (ATP) is documented. The reduction of bacterial ATP by an antimicrobial agent was determined to be a valid measure of drug effect in most cases. The effect of 12 antibiotics on 8 different bacterial species gave a 94 percent correlation with the standard Kirby-Buer-Agar disc diffusion method. A 93 percent correlation was obtained when the ATP assay method was applied directly to 50 urine specimens from patients with urinary tract infections. Urine samples were centrifuged first to that bacterial pellets could be suspended in broth. No primary isolation or subculturing was required. Mixed cultures in which one species was predominant gave accurate results for the most abundant organism. Since the method is based on an increase in bacterial ATP with time, the presence of leukocytes did not interfere with the interpretation of results. Both the incubation procedure and the ATP assays are compatible with automation.

Picciolo, G. L.↗
NASA NTRSDate not supplied

Space-Cent: Studying the Physiological and Anatomical Effects of Centrifugation and Head Down Tilt: 2021 Update

BACKGROUND The objective of this study was to evaluate the cerebral and ocular physiological and anatomical effects of head-down tilt bedrest (HDBR) with and without daily artificial gravity (AG) in healthy subjects to provide novel insights into possible countermeasures for the Spaceflight Associated Neuro-ocular Syndrome (SANS). Previous long-term studies using the spaceflight analog of strict 6° HDBR for 30 days revealed changes in ocular structure, including optic disc edema. The effects of an AG countermeasure using intermittent centrifugation to restore upright hydrostatic gradients and reduce the ocular and brain anatomical effects has not been explored. METHODS The effects of 60-day exposure to simulated microgravity (6° HDBR) on the cerebral, ocular and vestibular systems with and without the intervention of daily 30 minute exposure to AG (short-arm centrifugation) was evaluated in 24 healthy subjects. Non-invasive measurements of cerebral blood flow, intracranial blood volume (near infrared spectroscopy, NIRS), cerebral and ocular structure (MRI and optical coherence tomography), internal jugular vein area (ultrasound), lateral ventricular volumes (MRI), intraocular pressure, and sensorimotor/vestibular (i.e. balance and coordination) systems were made during various time-points during the bedrest study. Additional measures with ultrasound and NIRS were made during centrifugation. RESULTS The study began in Spring 2019 and all 24 subjects completed the testing by end of 2019. Data analysis continues. We report the following updated results for 2021. MRI showed significant decreases in carotid artery flow, increases in brain and CSF volumes, and aqueductal flow velocities in all subjects in HDT compared to baseline supine, but no effects of the short duration AG. All groups showed increased posterior globe flattening during bedrest compared to baseline, without protection from AG. As previously reported we observed chorioretinal folds for the first time in a bedrest analog, as well as optic disc edema, with no protection from the short-term AG. Data from the study is now being transmitted to the NASA LSDA which will allow for future research. CONCLUSIONS The 6 degree HDBR analog produced significant changes in the anatomical and physiological functions of the brain and eye in healthy subjects. Daily 30 minute exposure to AG with short-arm centrifugation had significant transient effects on physiology of the cerebrovascular system, but was not sufficient to prevent the anatomical effects of HDBR on the brain and eye.

E M Bershad↗
DOE OSTI2025

NFE-24-10417: Durability and Life-time Energy Performance Enhancement of Vapor Compression Systems

This project investigated the use of a Centrifugal Particle Separation (CPS) system to improve the durability and operational reliability of outdoor heat exchangers in vapor-compression based heating, ventilation, air conditioning, and refrigeration (HVACR) systems. The developed method introduced a novel dust and fouling mitigation approach by integrating an ambient air pre-cleaner that employs centrifugal forces to remove particulate matter from incoming air before it reaches the heat exchanger. By reducing the accumulation of dust and debris on coil surfaces, the CPS system aims to lower maintenance requirements and extend equipment lifespan. The project specifically involves coupling a centrifugal air pre-cleaner with a single-zone ductless mini-split system and evaluating its effectiveness in maintaining cleaner coil surfaces. Additionally, the study examined the impact of CPS integration on system energy performance. Through controlled experimental testing and performance monitoring, this work seeks to validate CPS-based air pre-cleaning as a practical, efficient solution for mitigating outdoor coil dust accumulation and fouling in both residential and commercial HVACR applications.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗