Search NASA⌕ Search

SEARCH · Search NASA

Results for “chlorophyll fluorescence”

Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 127 records · Page 7

EO-1 Hyperion Reflectance Time Series at Calibration and Validation Sites: Stability and Sensitivity to Seasonal Dynamics

This study evaluated Earth Observing 1 (EO-1) Hyperion reflectance time series at established calibration sites to assess the instrument stability and suitability for monitoring vegetation functional parameters. Our analysis using three pseudo-invariant calibration sites in North America indicated that the reflectance time series are devoid of apparent spectral trends and their stability consistently is within 2.5-5 percent throughout most of the spectral range spanning the 12-plus year data record. Using three vegetated sites instrumented with eddy covariance towers, the Hyperion reflectance time series were evaluated for their ability to determine important variables of ecosystem function. A number of narrowband and derivative vegetation indices (VI) closely described the seasonal profiles in vegetation function and ecosystem carbon exchange (e.g., net and gross ecosystem productivity) in three very different ecosystems, including a hardwood forest and tallgrass prairie in North America, and a Miombo woodland in Africa. Our results demonstrate the potential for scaling the carbon flux tower measurements to local and regional landscape levels. The VIs with stronger relationships to the CO2 parameters were derived using continuous reflectance spectra and included wavelengths associated with chlorophyll content and/or chlorophyll fluorescence. Since these indices cannot be calculated from broadband multispectral instrument data, the opportunity to exploit these spectrometer-based VIs in the future will depend on the launch of satellites such as EnMAP and HyspIRI. This study highlights the practical utility of space-borne spectrometers for characterization of the spectral stability and uniformity of the calibration sites in support of sensor cross-comparisons, and demonstrates the potential of narrowband VIs to track and spatially extend ecosystem functional status as well as carbon processes measured at flux towers.

Calibration↗

Capturing Complete Spatial Context in Satellite Observations of Greenhouse Gases

Scientific consensus from a 2015 pre-Decadal Survey workshop highlighted the essential need for a wide-swath (mapping) low earth orbit (LEO) instrument delivering carbon dioxide (CO2), methane (CH4), and carbon monoxide (CO) measurements with global coverage. OCO-2 pioneered space-based CO2 remote sensing, but lacks the CH4, CO and mapping capabilities required for an improved understanding of the global carbon cycle. The Carbon Balance Observatory (CARBO) advances key technologies to enable high-performance, cost-effective solutions for a space-based carbon-climate observing system. CARBO is a compact, modular, 15-30° field of view spectrometer that delivers high-precision CO2, CH4, CO and solar induced chlorophyll fluorescence (SIF) data with weekly global coverage from LEO. CARBO employs innovative immersion grating technologies to achieve diffraction-limited performance with OCO-like spatial (2 x 2 sq.km) and spectral (λ/Δλ≈ 20,000) resolution in a package that is >50% smaller, lighter and more cost-effective. CARBO delivers a 25- to 50-fold increase in spatial coverage compared to OCO-2 with no loss of detection sensitivity. Individual CARBO modules weigh < 20 kg, opening diverse new space-based platform opportunities.

Miller, Charles E.↗

Satellite-Detected Ocean Ecosystem Response to Volcanic Eruption in the Subarctic Northeast Pacific Ocean

Volcanic eruptions in the Aleutian archipelago during the summer of 2008 deposited large quantities of iron-laden ash to the Subarctic North Pacific Ocean. The surface ocean ecosystem response has been previously characterized using limited in situ and autonomous field measurements and numerical modeling, but basin-scale satellite remote sensing has been limited to a simple description of chlorophyll variability. Here, we show that the ecosystem response is a complex combination of phytoplankton biomass and physiology that can be described with satellite ocean color diagnostics such as the chlorophyll to carbon biomass ratio (Chl:Cphyto) and chlorophyll fluorescence yield. Together, these quantities outline a more complete picture of ecological response spanning unique signals of iron stress (and relief from), photo acclimation, changes in phytoplankton growth rate, increases in biomass, and timescales of decay for these processes.

Ocean Ecosystem↗

Remote Sensing of Diatom Bloom Succession

Marine diatoms are major biogeochemical and ecological “influencers” that contribute to a large fraction of the carbon export and supplying fisheries (Falkowski 2015). The fluxes of carbon transfer to the food web or to the deep ocean vary according to the stage of a diatom bloom (Du Toit 2018). Stages can be determined using inherent optical properties that reflect their physiological state, such as the chlorophyll fluorescence to particulate backscattering ratio (ChlF/b(sub bp), Cetinic et al. 2015). Identifying the bloom stage can potentially improve biogeochemical models of carbon export and fishery management. However, it is not yet possible to adequately determine the stage of phytoplankton blooms using satellites. Satellite-derived remote sensing reflectance R(sub rs)(𝜆) allow for remote identification of diatom blooms in the open ocean (Sathyendranath et al. 2004), and there are techniques to estimate the fluorescence quantum yield (𝜑) that, when high, can indicate the nutrient limitation that often takes place when blooms start to senesce (Behrenfeld et al. 2009). The goal of this study is to use the ratio between the normalized fluorescence line height from R(sub rs)(𝜆) (nFLH) and the particulate backscattering (b(sub bp)(443)) provided by satellites to identify exponentially growing and senescent diatom blooms from space.

Lange, Priscila K.↗

CARBO - The Carbon Observatory Instrument Suite - The Next Generation of Earth Observing Instruments for Global Monitoring of Carbon Gases

The Carbon Observatory Instrument Suite, or CARBO, consists of four carbon observing instruments sharing a common instrument bus, yet targeted for a particular wavelength band each with a unique science observation. They are: a) Instrument 1, wavelength centered at 756 nm for oxygen and solar-induced chlorophyll fluorescence (SIF) observations, b) Instrument 2, centered at 1629 nm, for carbon dioxide (CO2) and methane (CH4) observation, c) Instrument 3, centered at 2062 nm for carbon dioxide and d) Instrument 4, centered at 2328 for carbon monoxide (CO) and methane. From low-Earth orbit, these instruments have a field-of-view of 10 to 15 degrees, and a spatial resolution of 2 km square. These instruments have a spectral resolving power ranging from ten to twenty thousand, and can monitor columnaverage dry air mole fraction of carbon dioxide (XCO2) at 1.5 ppm, and methane (XCH4) at 7 ppb. These new instruments will advance the use of immersion grating technology in spectrometer instruments in order to reduce the size of the instrument, while improving performance. These compact, capable instruments are envisioned to be compatible with small satellites, yet modular to be configured to address the particular science questions at hand. Here we report on the current status of the instrument design and fabrication, focusing primarily on Instruments 1 and 2. We will describe the key science and engineering requirements and the instrument performance error budget. We will discuss the optical design with particular emphasis on the immersion grating, and the advantages this new technology affords compared to previous instruments. We will also discuss the status of the focal plane array and the detector electronics and housing. Finally, we report on a new approach – developed during this instrument design process - which enables simultaneous measurement of both orthogonal polarization states (S and P) over the field-of-view and optical bandpass. We believe this polarization sensing capability will enable science observations which were previously limited by instrumental and observational degeneracies. In particular: improved sensitivity to all species, better sensitivity to surface polarization effects, better constraints on aerosol scattering parameters, and superior discrimination of the vertical distribution of gases and aerosols.

Bernas, Michael↗

Satellite‐Detected Ocean Ecosystem Response to Volcanic Eruptions in the Subarctic Northeast Pacific Ocean

Volcanic eruptions in the Aleutian Archipelago during the summer of 2008 deposited large quantities of iron‐laden ash to the subarctic North Pacific Ocean. The surface ocean ecosystem response has been previously characterized using limited in situ and autonomous field measurements and numerical modeling, but basin‐scale satellite remote sensing has been limited to a simple description of chlorophyll variability. Here, we show that the ecosystem response is a complex combination of phytoplankton biomass and physiology that can be described with satellite ocean color diagnostics such as the chlorophyll‐to‐carbon biomass ratio (Chl:Cphyto) and chlorophyll fluorescence yield. Together, these quantities outline a more complete picture of ecological responses spanning unique signals of iron stress (and relief from), photo acclimation, changes in phytoplankton growth rate, increases in biomass, and timescales of decay for these processes.

T.K. Westberry↗

Validation Assessment for the Soil Moisture Active Passive (SMAP) Level 4 Carbon (L4_C) Data Product Version 5

The post-launch Cal/Val phase of the SMAP mission is guided by two primary objectives for each science product team: 1) to calibrate, verify, and improve the performance of the science algorithms, and 2) validate accuracies of the science data products as specified in the SMAP Level-1 mission science requirements. Algorithm science and product maintenance activities during the SMAP extended mission phase have also involved periodic algorithm calibration and product refinements to maintain or enhance product consistency and performance as well as science utility. This report provides an assessment of the latest (Version 5) SMAP Level 4 Carbon (L4_C) product. The L4_C Version 5 (v5) global record now spans more than six years (March 2015 – present) of SMAP operations and has benefited from five major reprocessing updates to the operational product. These reprocessing events and L4_C product release updates have incorporated various algorithm refinements and calibration adjustments to account for similar refinements to the upstream GEOS land model assimilation system, SMAP brightness temperatures, and MODIS vegetation inputs used for L4_C processing. The SMAP L4_C algorithms utilize a terrestrial carbon flux model informed by daily surface and root zone soil moisture information contributed from the SMAP Level 4 Soil Moisture (L4_SM) product along with optical remote sensing-based (e.g. MODIS-based) land cover and canopy fractional photosynthetic active radiation (fPAR), and other ancillary biophysical data. The carbon flux model estimates global daily net ecosystem CO2 exchange (NEE) and the component carbon fluxes, namely, vegetation gross primary production (GPP) and soil heterotrophic respiration (Rh). Other L4_C product elements include surface (~0-5 cm depth) soil organic carbon (SOC) stocks and associated environmental constraints to these processes, including soil moisture-related controls on GPP and ecosystem respiration (Kimball et al. 2014, Jones et al. 2017). The L4_C product addresses SMAP carbon cycle science objectives by: 1) providing a direct link between terrestrial carbon fluxes and underlying freeze/thaw and soil moisture-related constraints to these processes, 2) documenting primary connections between terrestrial water, energy and carbon cycles, and 3) improving understanding of terrestrial carbon sink activity. The SMAP L4_C algorithms and operational product are mature and at a CEOS Validation Stage 4 level (Jackson et al. 2012) based on extensive validation of the multi-year record against a diverse array of independent benchmarks, well characterized global performance, and systematic refinements gained from five major reprocessing events. There are no Level-1 mission science requirements for the L4_C product; however, self-imposed requirements have been established focusing on NEE as the primary product field for validation, and on demonstrating L4_C accuracy and success in meeting product science requirements (Jackson et al. 2012). The other L4_C product fields also have strong utility for carbon science applications (e.g., Liu et al. 2019, Endsley et al. 2020); however, analysis of these other fields is considered secondary relative to primary validation activities focusing on NEE. The L4_C targeted accuracy requirements are to meet or exceed a mean unbiased root-mean-square error (ubRMSE, or standard deviation of the error) for NEE of 1.6 g C m-2 d-1 and 30 g C m-2 yr-1, emphasizing northern (≥45°N) boreal and arctic ecosystems; this accuracy is similar to that of tower eddy covariance measurement-based observations (Baldocchi 2008). Methods used for the latest v5 L4_C product performance and validation assessment have been established from the SMAP Cal/Val plan and previous studies (Jackson et al. 2012, Jones et al. 2017) and include: 1) consistency evaluations of the product fields against earlier product releases (version 4 or earlier); 2) comparisons of daily carbon flux estimates with independent tower eddy 4covariance measurement-based daily carbon (CO2) flux observations from core tower validation sites (CVS); and 3) consistency checks against other global carbon products, including soil carbon inventory records, global GPP records derived from tower observation upscaling methods, and satellite-based observations of canopy solar induced chlorophyll fluorescence (SIF) as a surrogate for GPP. Metrics used to evaluate relative agreement between L4_C product fields and observational benchmarks include correlation (r-value), RMSE differences, bias and model sensitivity diagnostics. Following these validation criteria, the present report provides a validation assessment of the latest L4_C product release (v5). Detailed descriptions of the L4_C algorithm and additional global product accuracy and performance results are given elsewhere (Jones et al. 2017, Endsley et al. 2020). The v5 L4_C product replaces earlier product versions and continues to show: (i) accuracy and performance levels meeting or exceeding SMAP L4_C science requirements; (ii) improvement over the previous product version (version 4); and (iii) suitability for a diversity of science applications. Example L4_C applications from the recent literature include clarifying environmental trends and controls on the northern terrestrial carbon sink (Liu et al. 2019), diagnosing drought-related impacts on ecosystem productivity (Li et al. 2020), and regional monitoring of cropland conditions for projecting annual yields (Wurster et al. 2020). 2EXPECTED L4_C ALGORITHM AND PRODUCTPERFORMANCE The L4_C algorithm performance, including variance and uncertainty estimates of model outputs, was determined during the mission pre-launch phase through spatially explicit model sensitivity studies using available model inputs similar to those currently being used for operational production and evaluating the resulting model simulations over the observed range of northern (≥45 °N) and global conditions (Kimball et al. 2012, Entekhabi et al. 2014). The L4_C algorithm options were also evaluated during the mission prelaunch phase, including deriving canopy fPAR from lower order NDVI (Normalized Difference Vegetation Index) inputs in lieu of using MODIS (MOD15) fPAR; and including an explicit model representation of boreal fire disturbance recovery impacts. These results indicated that the L4_C accuracy requirements (i.e., NEE ubRMSE ≤ 30 g C m-2 yr-1or ≤ 1.6 g C m-2 d-1) could be met from the baseline algorithms over more than 82% and 89% of global and northern vegetated land areas, respectively (Yi et al. 2013, Kimball et al. 2014). The global L4_C algorithm error budget for NEE derived during the mission prelaunch phase indicated that the estimated NEE ubRMSE uncertainty is proportional to GPP and is therefore larger in higher biomass productivity areas, including forests and croplands (Kimball et al. 2014). Likewise, NEE ubRMSE uncertainty is expected to be lower in less-productive areas, including grasslands and shrublands. Expected model NEE ubRMSE levels were also generally within targeted accuracy levels for characteristically less-productive boreal and Arctic biomes, even though relative model error as a proportion of total productivity (NEE RMSE / GPP) may be large in these areas. The estimated NEE uncertainty was lower than expected in some warmer tropical high biomass productivity areas (e.g. Amazon rainforest) because of reduced low temperature and moisture constraints to the L4_C respiration calculations so that the bulk of model uncertainty is contributed by GPP in these areas. Model NEE uncertainty in the African Congo was estimated to

SMAP↗

MONITORING ECO-HYDROLOGICAL SPRING ONSET OVER ALASKA AND NORTHERN CANADA WITH COMPLEMENTARY SATELLITE REMOTE SENSING DATA

More than half of the global land area undergoes seasonal freeze/thaw (FT) transitions in spring. Spatial patterns and timing of spring thawing influence eco-hydrological processes and landscape moisture availability over arctic and boreal ecosystems. The seasonal progression of spring thawing coincides with warmer temperatures, snowmelt, and a rapid increase in soil moisture, which initiates the growing season for ecosystem productivity. In this study, we utilize complementary satellite observations to determine the pattern and order of occurrence in landscape thawing, soil moisture increase, and ecosystem productivity that collectively define the eco-hydrological spring onset across Alaska and Northern Canada. Satellite data utilized include landscape FT status from SMAP and AMSR-2, OCO-2 derived solar-induced chlorophyll fluorescence (GOSIF), and gross primary production (GPP) and soil moisture from SMAP.

Youngwook Kim↗

TLSLEAF: Automatic Leaf Angle Estimates From Single-Scan Terrestrial Laser Scanning

Leaf angle distribution (LAD) in forest canopies affects estimates of leaf area, light interception, and global-scale photosynthesis, but is often simplified to a single theoretical value. Here, we present TLSLeAF (Terrestrial Laser Scanning Leaf Angle Function), an automated open-source method of deriving LADs from terrestrial laser scanning. TLSLeAF produces canopy-scale leaf angle and LADs by relying on gridded laser scanning data. The approach increases processing speed, improves angle estimates, and requires minimal user input. Key features are automation, leaf–wood classification, beta parameter output, and implementation in R to increase accessibility for the ecology community. TLSLeAF precisely estimates leaf angle with minimal distance effects on angular estimates while rapidly producing LADs on a consumer-grade machine. We challenge the popular spherical LAD assumption, showing sensitivity to ecosystem type in plant area index and foliage profile estimates that translate to c. 25% and c. 11% increases in canopy net photosynthesis (c. 25%) and solar-induced chlorophyll fluorescence (c. 11%). TLSLeAF can now be applied to the vast catalog of laser scanning data already available from ecosystems around the globe. The ease of use will enable widespread adoption of the method outside of remote-sensing experts, allowing greater accessibility for addressing ecological hypotheses and large-scale ecosystem modeling efforts.

3D↗

Monitoring Eco-Hydrological Spring Onset Over Alaska and Northern Canada With Complementary Satellite Remote Sensing Data

More than half of the global land area undergoes seasonal freeze/thaw (FT) transitions in spring. Spatial patterns and timing of spring thawing influence eco-hydrological processes and landscape moisture availability over arctic and boreal ecosystems. The seasonal progression of spring thawing coincides with warmer temperatures, snowmelt, and a rapid increase in soil moisture, which initiates the growing season for ecosystem productivity. In this study, we utilize complementary satellite observations to determine the pattern and order of occurrence in landscape thawing, soil moisture increase, and ecosystem productivity that collectively define the eco-hydrological spring onset across Alaska and Northern Canada. Satellite data utilized include landscape FT status from SMAP and AMSR-2, OCO-2 derived solar-induced chlorophyll fluorescence (GOSIF), and gross primary production (GPP) and soil moisture from SMAP. The resulting spring onset maps showed spring thawing as the precursor to growing season onset, indicated by a rapid rise in available soil moisture and GPP. Our results indicated an average spring transition period of 3±2 (SD) weeks between initial landscape thawing and growing season onset. A rapid increase in soil moisture generally followed landscape thawing but occurred before the subsequent seasonal rise in GPP. Spring onset generally occurred earlier in boreal forest (DOY 102±14) than arctic tundra (DOY 124±22).

Derksen, Chris↗

Leaf Functional Traits in Relation to Species Composition in an Arctic–Alpine Tundra Grassland

The relict arctic–alpine tundra provides a natural laboratory to study the potential impacts of climate change and anthropogenic disturbance on tundra vegetation. The Nardus stricta-dominated relict tundra grasslands in the Krkonoše Mountains have experienced shifting species dynamics over the past few decades. Changes in species cover of the four competing grasses—Nardus stricta, Calamagrostis villosa, Molinia caerulea, and Deschampsia cespitosa—were successfully detected using orthophotos. Leaf functional traits (anatomy/morphology, element accumulation, leaf pigments, and phenolic compound profiles), were examined in combination with in situ chlorophyll fluorescence in order to shed light on their respective spatial expansions and retreats. Our results suggest a diverse phenolic profile in combination with early leaf expansion and pigment accumulation has aided the expansion of C. villosa, while microhabitats may drive the expansion and decline of D. cespitosa in different areas of the grassland. N. stricta—the dominant species—is retreating, while M. caerulea did not demonstrate significant changes in territory between 2012 and 2018. We propose that the seasonal dynamics of pigment accumulation and canopy formation are important factors when assessing potential “spreader” species and recommend that phenology be taken into account when monitoring grass species using remote sensing.

Lena Hunt↗

From Remotely-Sensed SIF to Ecosystem Structure, Function, and Service: Part I - Harnessing Theory

Solar-induced chlorophyll fluorescence (SIF) is a remotely sensed optical signal emitted during the light reactions of photosynthesis. The past two decades have witnessed an explosion in availability of SIF data at increasingly higher spatial and temporal resolutions, sparking applications in diverse research sectors (e.g., ecology, agriculture, hydrology, climate, and socioeconomics). These applications must deal with complexities caused by tremendous variations in scale and the impacts of interacting and superimposing plant physiology and three-dimensional vegetation structure on the emission and scattering of SIF. At present, these complexities have not been overcome. To advance future research, the two companion reviews aim to (1) develop an analytical framework for inferring terrestrial vegetation structures and function that are tied to SIF emission, (2) synthesize progress and identify challenges in SIF research via the lens of multi-sector applications, and (3) map out actionable solutions to tackle these challenges and offer our vision for research priorities over the next 5–10 years based on the proposed analytical framework. This paper is the first of the two companion reviews, and theory oriented. It introduces a theoretically rigorous yet practically applicable analytical framework. Guided by this framework, we offer theoretical perspectives on three overarching questions: (1) The forward (mechanism) question—How are the dynamics of SIF affected by terrestrial ecosystem structure and function? (2) The inference question: What aspects of terrestrial ecosystem structure, function, and service can be reliably inferred from remotely sensed SIF and how? (3) The innovation question: What innovations are needed to realize the full potential of SIF remote sensing for real-world applications under climate change? The analytical framework elucidates that process complexity must be appreciated in inferring ecosystem structure and function from the observed SIF; this framework can serve as a diagnosis and inference tool for versatile applications across diverse spatial and temporal scales.

carbon fluxes↗

The Validity Chlorophyll-a Estimation by Sun Induced Fluorescence in Estuarine Waters: An Analysis of Long-term (2003-2011) Water Quality Data from Tampa Bay, Florida (USA)

Satellite observation of phytoplankton concentration or chlorophyll-a is an important characteristic, critically integral to monitoring coastal water quality. However, the optical properties of estuarine and coastal waters are highly variable and complex and pose a great challenge for accurate analysis. Constituents such as suspended solids and dissolved organic matter and the overlapping and uncorrelated absorptions in the blue region of the spectrum renders the blue-green ratio algorithms for estimating chlorophyll-a inaccurate. Measurement of sun-induced chlorophyll fluorescence, on the other hand, which utilizes the near infrared portion of the electromagnetic spectrum, may provide a better estimate of phytoplankton concentrations. While modelling and laboratory studies have illustrated both the utility and limitations of satellite baseline algorithms based on the sun induced chlorophyll fluorescence signal, few have examined the empirical validity of these algorithms using a comprehensive long term in situ data set. In an unprecedented analysis of a long term (2003-2011) in situ monitoring data from Tampa Bay, Florida (USA), we assess the validity of the FLH product from the Moderate Resolution Imaging Spectrometer (MODIS) against chlorophyll ]a and a suite of water quality parameters taken in a variety of conditions throughout a large optically complex estuarine system. A systematic analysis of sampling sites throughout the bay is undertaken to understand how the relationship between FLH and in situ chlorophyll-a responds to varying conditions within the estuary including water depth, distance from shore and structures and eight water quality parameters. From the 39 station for which data was derived, 22 stations showed significant correlations when the FLH product was matched with in situ chlorophyll-alpha data. The correlations (r2) for individual stations within Tampa Bay ranged between 0.67 (n=28, pless than 0.01) and-0.457 (n=12, p=.016), indicating that for some areas within the Bay, FLH can be a good predictor of chlorophyll-alpha concentration and hence a useful tool for the analysis of water quality. Overall, the results show a 106% increase in the validity of chlorophyll -a concentration estimates using FLH over the standard the blue-green OC3M algorithm. This analysis also illustrates that the correlations between FLH and in situ chlorophyll -a measurements increases with increasing water depth and distance of the monitoring sites from both the shore and structures. However, due to confounding factors related to the complexity of the estuarine system, a linear improvement in the FLH to chlorophyll ]a relationship was not clearly noted with increasing depth and distance from shore alone. Correlations of FLH with turbidity, nutrients (total nitrogen and total phosphorous) biological oxygen demand, salinity, sea surface temperature correlated positively with FLH concentrations, while dissolved oxygen and pH showed negative correlations. Principle component analyses are employed to further describe the relationships between the multivariate water quality parameters and the FLH product. The majority of sites with higher and very significant correlations (pless than 0.01) also showed high correlation values for nutrients, turbidity and biological oxygen demand. These sites were on average in greater than seven meters of water and over five kilometers from shore. A thorough understanding of the relationship between the MODIS FLH product and in situ water quality parameters will enhance our understanding of the accuracy MODIS fs global FLH algorithm and assist in optimizing its calibration for use in monitoring the quality of estuarine and coastal waters worldwide.

Moreno-Madrinan, Max Jacobo↗

Laboratory studies of in vivo fluorescence of phytoplankton

A lidar system is developed that uses four selected excitation wavelengths to induce chlorophyll 'a' fluorescence which is indicative of both the concentration and diversity of phytoplankton. The operating principles of the system and the results of measurements of phytoplankton fluorescence in a controlled laboratory environment are presented. A comparative study of results from lidar fluorosensor laboratory tank tests using representative species of phytoplankton in single and multispecies cultures from each of four color groups reveals that (1) there is good correlation between the fluorescence of chlorophyll 'a' remotely simulated and detected by the lidar system and in-situ measurements using four similar excitation wavelengths in a flow-through fluorometer; (2) good correlation exists between the total chlorophyll 'a' calculated from lidar-fluorosensor data and measurements obtained by the Strickland-Parsons method; and (3) the lidar fluorosensor can provide an index of population diversity.

Brown, C. A., Jr.↗

An algorithm for computing chlorophyll-a concentrations using a dual-frequency fluorosensor

An algorithm to be used on data from a dual-frequency fluorosensor (i.e. one using two wavelengths for excitation of chlorophyll-a fluorescence) to compute total chlorophyll-a concentration and to partition that chlorophyll between two color groups present in a mixed phytoplankton population is described. The algorithm is based on laboratory and field-testing experience gained with the airborne lidar oceanographic probing experiment fluorosensor.

Campbell, J. W.↗

A comparison of methods for the measurement of the absorption coefficient in natural waters

In the spring of 1992 an optical closure experiment was conducted at Lake Pend Orielle, Idaho. A primary objective of the experiment was to compare techniques for the measurement of the spectral absorption coefficent and other inherent optical properties of natural waters. Daily averages of absorption coefficents measured using six methods are compared at wavelengths of 456, 488, and 532 nm. Overall agreement was within 40% at 456 nm and improved with increasing wavelength to 25% at 532 nm. These absorption measurements were distributed over the final 9 days of the experiement, when bio-optical conditions in Lake Pend Oreille (as indexed by the beam attenuation coefficent c(sub p)(660) and chlorophyll a fluorescence profiles) were representative of those observed throughout the experiment. However, profiles of stimulated chlorophyll a fluorescence and beam transmission showed that bio-optical properties in the lake varied strongly on all time and space scales. Therefore environmental variabilty contributed significantly to deviations between daily mean absorption coefficients measured using the different techniques.

Pegau, W. Scott↗

Early quantitative method for measuring germination in non-green spores of Dryopteris paleacea using an epifluorescence-microscope technique

A method is described to determine germination by blue-light excited red fluorescence in the positively photoblastic spores of Dryopteris paleacea Sw. This fluorescence is due to chlorophyll as evidenced from 1) a fluorescence-emission spectrum in vivo, where a bright fluorescence around 675 nm is obtained only in red light (R)-irradiated spores and 2) in vitro measurements with acetone extracts prepared from homogenized spores. Significant amounts of chlorophyll can be found only in R-treated spores; this chlorophyll exhibits an emission band around 668 nm, when irradiated with 430 nm light at 21 degrees C. Compared to other criteria for germination, such as swelling of the cell, coat splitting, greening, and rhizoid formation, which require longer periods after induction for their expression, chlorophyll fluorescence can be used to quantify germination after two days. This result is confirmed by fluence-response curves for R-induced spore germination; the same relationship between applied R and germination is obtained by the evaluation with the epifluorescence method 2 days after the light treatment as compared with the evaluation with bright-field microscopy 5 days after the inducing R. Using this technique we show for the first time that Ca2+ contributes to the signal-transduction chain in phytochrome-mediated chlorophyll synthesis in spores of Dryopteris paleacea.

NASA Discipline Plant Biology↗

Remote sensing of phytoplankton density and diversity in Narragansett Bay using an airborne fluorosensor

An aircraft-borne remote system is presented that utilizes narrow-band light from multiple dye lasers to excite selected algae photopigments and then measures the resultant flourescence emitted from chlorophyll a at 685 nm. Tests were conducted with both pure and mixed cultures of marine algae from a series of field tests taken from piers and bridges of Narragansett Bay, and a prototype remote fluorosensor was flown over the Bay during the 1978 winter-spring diatom bloom. Remote fluorescence obtained at hover points over sea-truth stations showed correlations with in situ fluorescence, total chlorophyll a, and cell count. It was concluded that the ratio of remote fluorescence to direct chlorophyll a concentration was less variable than expected, and the distribution of total chlorophyll a between two major photoplankton color groups showed three distinct areas, within the Bay, of green and golden-brown species.

Farmer, F. H.↗