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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 127 records · Page 7
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NASA NTRS1992

Effect of microgravity and mechanical stimulation on the in vitro mineralization and resorption of fetal mouse long bones (7-IML-1)

Mechanical forces play an important role in the differentiation, growth, and remodeling of skeletal tissues. An increase in the normal loading pattern of the skeleton leads to an increase in bone mass. An overall decrease in the functional load exerted on the skeleton produces mineral loss and osteoporosis. However, the responses of the skeletal tissue cells to various loading conditions are still largely unresolved, as is the mechanism of the cellular response to changed mechanical environment. Using an in vitro approach, we hope to avoid some problems encountered in the use of in vivo animal and man models, which have been extensively used in the past. In a number of experiments we have demonstrated that 16 and 17 day old fetal mouse long bone rudiments (metatarsalia), cultured in a liquid culture medium, are very suitable to study mineralization and resorption, respectively. We have also demonstrated that under hydrostatic compression, mineralization is increased while resorption is decreased. Culture of long bone rudiments under noncompressed control conditions can be regarded as a situation of partial unloading, showing some phenomena of a disuse situation. Under microgravity conditions, responses of osteoblasts and chondrocytes (involved in mineralization) and osteoclasts (involved in mineral resorption), to culture with and without compression, may be much more outspoken. This will have advantages for the study and the interpretation of the role of cellular events in the process of mineralization and resorption of developing skeletal tissues under various loading conditions. The BONES Experiment is carried out in four type I/O and four type I/E containers. Various aspects of the investigation are discussed.

Veldhuijzen, J. Paul↗
NASA NTRS2014

Limb Correction of RGB Composite Imagery for Improved Interpretation

RGB (Red‐Green‐Blue) imagery, created by integrating several spectral channels into one composite image, is currently used by the operational weather community to aid in quick, real‐time analysis of atmospheric processes. However, the limb effect - a result of an increasing optical path length of the absorbing atmosphere between the satellite and the earth as scan angle increases - interferes with the qualitative interpretation of RGB composites at large scan angles. It also makes the comparison of similar products from multiple satellite sensors difficult. Recent work has indicated that correcting for the limb effect in the basic channel imagery using simple statistical relationships greatly improves the utility of the derived RGB imagery. However, it is hypothesized that the limb correction coefficients vary with respect to latitude, season, cloud cover, and surface albedo. This poster will highlight an improved approach to the limb correction of RGB imagery using varying coefficients. The Joint Center for Satellite and Data Assimilation (JCSDA) Community Radiative Transfer Model (CRTM) was used to simulate top of atmosphere brightness temperatures at varying scan angles for infrared channels corresponding to the Aqua and Terra Moderate Resolution Imaging Spectroradiometer (MODIS), Suomi NPP Visible Infrared Imaging Radiometer Suite (VIIRS), and Meteosat‐10 Spinning Enhanced Visible and Infrared Imager (SEVIRI) sensors. A subset of European Center for Medium‐Range Weather Forecasts (ECMWF) temperature, specific humidity, and ozone mixing ratio profiles from March 2013 through February 2014 were used as input to the CRTM. The simulated brightness temperatures were used to determine the best fit slope of the linear relationship between the natural log of the cosine of the scan angle and the difference of the simulated brightness temperature at nadir and on the limb. The correction coefficients were then analyzed for variability with respect to latitude, season, cloud cover, and surface type and used to produce improved limb corrected imagery. Applications of the results will be presented.

Elmer, Nicholas↗
NASA NTRSDate not supplied

The HEROIX Broadband X-Ray Space Telescope Mission Concept

The High EneRgy Observatory for Imaging X-rays (HEROIX) Medium-Class Mission concept envisions a next-generation broadband X-ray observatory designed to meet the astrophysics community’s need for high-angular-resolution studies of the Cosmic X-ray Background. Building on the science legacy of NASA’s NuSTAR mission and more than 30 years of advances in replicated NiCo shell technology at NASA Marshall, HEROIX proposes a space observatory comprising four co-aligned telescopes achieving 5 arcsecond half-power diameter (HPD) angular resolution. This capability, approached by the resolution demonstrated in the FOXSI-4 sounding rocket mission and under development through the SuperHERO balloon mission, will be extended to energies up to 80 keV through multilayer coatings. Marshall’s inhouse precision optics manufacturing enables an integrated effective area of 380 cm² at 30 keV, which, combined with high-resolution optics, opens new windows into extreme astrophysical non-thermal processes.

Nick Thomas↗
NASA NTRS1995

Turbulence in the solar wind: Kinetic effects

Although a casual look at the fluctuating magnetic and velocity fields in the solar wind may be reminiscent of a chaotic and disordered flow, there is, nonetheless. considerable organization and structure in the temporal and spatial evolution of those fluctuations. Much of that evolution is controlled by processes operating on rather large scales for example, in the inner heliosphere, the fluctuations in magnetic and velocity are highly correlated in the sense of outward propagating Alfven waves. This correlation can be destroyed both in time and distance by the velocity gradients present between fast and slow streams and by other nonlinear processes which stir the medium, producing a turbulent cascade of energy from large to small scales. Many aspects of this turbulent evolution can be described using fluid models; however, at some scale the fluid approximation breaks down and a more detailed paradigm is necessary. The breakdown is evident in the power spectrum of magnetic fluctuations at scales approaching the wavelength of ion cyclotron waves. At those scales, as evident in Mariner 10 and other magnetometer data, the spectrum bends over and the fluctuations damp, possibly heating the ambient plasma. Some evidence for heating of the solar wind is present in the Voyager data. Fluid models can be modified to some extent to incorporate aspects of a kinetic treatment. This is done by modifying the dissipation terms in the fluid equations and by including extra terms, such as the Hall term. As the scale lengths of phenomena shrink further and approach the spatial and temporal scales characteristic of electron phenomena, the fluid description must be abandoned altogether and a fully kinetic treatment is required. One example is the generation of Langmuir solitons produced by the electron beams that generate type 3 solar radio bursts.

Goldstein, M. L.↗
NASA NTRS2005

Shared Voyage: Learning and Unlearning from Remarkable Projects

Shared Voyage is about four remarkable projects: the Advanced Composition Explorer (NASA), the Joint Air-to-Surface Standoff Missile (U.S. Air Force), the Pathfinder Solar-Powered Airplane (NASA), and the Advanced Medium Range Air-to-Air Missile (U.S.Air Force). Each project is presented as a case study comprised of stories collected from key members of the project teams. The stories found in the book are included with the purpose of providing an effective learning source for project management, encouraging the unlearning of outdated project management concepts, and enhancing awareness of the contexts surrounding different projects. Significantly different from project concepts found in most project management literature, Shared Voyage highlights concepts like a will to win, a results-oriented focus, and collaboration through trust. All four project teams researched in this study applied similar concepts; however, they applied them differently, tailoring them to fit the context of their own particular projects. It is clear that the one best way approach which is still the prevailing paradigm in project management literature should be replaced by a new paradigm: Even though general project management principles exist, their successful application depends on the specifics of the situation.

Laufer, Alexander↗
NASA NTRS1996

Sound Transmission through Cylindrical Shell Structures Excited by Boundary Layer Pressure Fluctuations

This paper examines sound transmission into two concentric cylindrical sandwich shells subject to turbulent flow on the exterior surface of the outer shell. The interior of the shells is filled with fluid medium and there is an airgap between the shells in the annular space. The description of the pressure field is based on the cross-spectral density formulation of Corcos, Maestrello, and Efimtsov models of the turbulent boundary layer. The classical thin shell theory and the first-order shear deformation theory are applied for the inner and outer shells, respectively. Modal expansion and the Galerkin approach are used to obtain closed-form solutions for the shell displacements and the radiation and transmission pressures in the cavities including both the annular space and the interior. The average spectral density of the structural responses and the transmitted interior pressures are expressed explicitly in terms of the summation of the cross-spectral density of generalized force induced by the boundary layer turbulence. The effects of acoustic and hydrodynamic coincidences on the spectral density are observed. Numerical examples are presented to illustrate the method for both subsonic and supersonic flows.

Tang, Yvette Y.↗
NASA NTRS2013

Predicting Pilot Behavior in Medium Scale Scenarios Using Game Theory and Reinforcement Learning

Effective automation is critical in achieving the capacity and safety goals of the Next Generation Air Traffic System. Unfortunately creating integration and validation tools for such automation is difficult as the interactions between automation and their human counterparts is complex and unpredictable. This validation becomes even more difficult as we integrate wide-reaching technologies that affect the behavior of different decision makers in the system such as pilots, controllers and airlines. While overt short-term behavior changes can be explicitly modeled with traditional agent modeling systems, subtle behavior changes caused by the integration of new technologies may snowball into larger problems and be very hard to detect. To overcome these obstacles, we show how integration of new technologies can be validated by learning behavior models based on goals. In this framework, human participants are not modeled explicitly. Instead, their goals are modeled and through reinforcement learning their actions are predicted. The main advantage to this approach is that modeling is done within the context of the entire system allowing for accurate modeling of all participants as they interact as a whole. In addition such an approach allows for efficient trade studies and feasibility testing on a wide range of automation scenarios. The goal of this paper is to test that such an approach is feasible. To do this we implement this approach using a simple discrete-state learning system on a scenario where 50 aircraft need to self-navigate using Automatic Dependent Surveillance-Broadcast (ADS-B) information. In this scenario, we show how the approach can be used to predict the ability of pilots to adequately balance aircraft separation and fly efficient paths. We present results with several levels of complexity and airspace congestion.

Game Theory↗
NASA NTRS2010

Optical and Microphysical Retrievals of Marine Stratocumulus Clouds off the Coast of Namibia from Satellite and Aircraft

Though the emphasis of the Southern Africa Regional Science Initiative 2000 (SAFARI-2000) dry season campaign was largely on emission sources and transport, the assemblage of aircraft (including the high altitude NASA ER-2 remote sensing platform and the University of Washington CV-580, UK MRF C-130, and South African Weather Bureau JRA in situ aircrafts) provided a unique opportunity for cloud studies. Therefore, as part of the SAFARI initiative, investigations were undertaken to assess regional aerosol-cloud interactions and cloud remote sensing algorithms. In particular, the latter part of the experiment concentrated on marine boundary layer stratocumulus clouds off the southwest coast of Africa. Associated with cold water upwelling along the Benguela current, the Namibian stratocumulus regime has received limited attention but appears to be unique for several reasons. During the dry season, outflow of continental fires and industrial pollution over this area can be extreme. From below, upwelling provides a rich nutrient source for phytoplankton (a source of atmospheric sulfur through DMS production as well as from decay processes). The impact of these natural and anthropogenic sources on the microphysical and optical properties of the stratocumulus is unknown. Continental and Indian Ocean cloud systems of opportunity were also studied during the campaign. SAFARI 2000 aircraft flights off the coast of Namibia were coordinated with NASA Terra Satellite overpasses for synergy with the Moderate Resolution Imaging Spectroradiometer (MODIS) and other Terra instruments. MODIS was developed by NASA and launched onboard the Terra spacecraft on December 18, 1999 (and Aqua spacecraft on May 4, 2002). Among the remote sensing algorithms developed and applied to this sensor are cloud optical and microphysical properties that include cloud thermodynamic phase, optical thickness, and effective particle radius of both liquid water and ice clouds. The archived products from these algorithms have applications in climate change studies, climate modeling, numerical weather prediction, and fundamental atmospheric research. The archived MODIS Collection 5 cloud products processing stream will be used to analyze low water cloud scenes off the Namibian and Angolan coasts during SAFARI 2000 time period, as well as other years. Pixel-level Terra and Aqua MODIS retrievals (l. km spatial resolution at nadir) and gridded (1' uniform grid) statistics of cloud optical thickness and effective particle radius will be presented, including joint probability distributions between the two quantities. In addition, perspectives from the MODIS Airborne Simulator, which flew on the ER-2 during SAFARI 2000 providing high spatial resolution retrievals (50 m at nadir), will be presented as appropriate. The H-SAF Program requires an experimental operational European-centric Satellite Precipitation Algorithm System (E-SPAS) that produces medium spatial resolution and high temporal resolution surface rainfall and snowfall estimates over the Greater European Region including the Greater Mediterranean Basin. Currently, there are various types of experimental operational algorithm methods of differing spatiotemporal resolutions that generate global precipitation estimates. This address will first assess the current status of these methods and then recommend a methodology for the H-SAF Program that deviates somewhat from the current approach under development but one that takes advantage of existing techniques and existing software developed for the TRMM Project and available through the public domain.

Platnick, Steven E.↗
NASA NTRS2021

Assessing Ceres’ Past and Current Habitability

Dwarf planet Ceres is a compelling target as an evolved ocean world with, at least, regional brine reservoirs and potentially ongoing geological activity. Situated in the Main Belt of asteroids, Ceres is the most water-rich body in the inner solar system (in relative abundance) and is accessible enough for a sample return with the resources of a typical medium-class (New Frontiers) NASA mission. NASA’s Dawn mission at Ceres revealed the presence of liquid, brine-driven activity, organic matter, and a rich salt chemistry. With this evidence, the overarching goals of the mission concept presented herein are to quantify Ceres’ current habitability potential and origin. A sample return from young evaporite deposits in Occator crater offers greater science return than an in situ exploration mission by enabling high-resolution analysis of (1) organic matter expected from terrestrial and chondritic analogs that are trapped in salt minerals and (2) isotopes of refractory elements for a similar cost and less science risk. The sample return concept would be executed with a single flight system due to Ceres’ relative proximity to Earth and low gravity. Solar electric propulsion was identified as the most cost-effective approach for getting to Ceres and back. De-orbiting, landing, and takeoff are performed with a throttleable monopropellant hydrazine system. The solar arrays are stowed prior to landing and takeoff. Sample acquisition builds on the pneumatic system designed by Honeybee Robotics. The sample return mission concept relies on the availability of key technologies: an enhanced landing vision system leveraging investments from Mars 2020; retractable/redeployable solar arrays, which have been demonstrated on the International Space Station but not at Ceres’ gravity; and an emerging design from upcoming missions for sample transfer from the pneumatic sampling system to the sample return capsule. Return of a sample of mass ~100 g from Ceres, maintained at ≤ -20°C, is without precedent for any currently advocated Ocean World mission, enabling a vast range of experimental techniques back on Earth with sensitivities and accuracies far beyond those feasible with in situ instruments. A sample of this size also enables analyses to benefit from techniques that will become available in the future.

Casillas, Raul Polit↗
NASA NTRS2009

A Model of Thermal Conductivity for Planetary Soils: Theory for Cemented Soils - 2

A numerical model of heat conduction through particulate media made of spherical grains cemented by various bonding agents is presented. The pore-filling gas conductivity, volume fraction, and thermal conductivity of the cementing phase are tunable parameters. Cement fractions <0.001-0.01% in volume have small effects on the soil bulk thermal conductivity. A significant conductivity increase (factor 3-8) is observed for bond fractions of 0.01 to 1% in volume. In the 1 to 15% bond fraction domain, the conductivity increases continuously but less intensely (25-100% conductivity increase compared to a 1% bond system). Beyond 15% of cements, the conductivity increases vigorously and the bulk conductivity rapidly approaches that of bedrock. The composition of the cements (i.e. conductivity) has little influence on the bulk thermal inertia of the soil, especially if the volume of bond <10%. These results indicate that temperature measurements are sufficient to detect cemented soils and quantify the amount of cementing phase, but the mineralogical nature of the bonds and the typical grain size are unlikely to be determined from orbit. On Mars, a widespread surface unit characterized by a medium albedo (0.19-0.26) and medium/high thermal inertia (200-600 J s(0.5)/sq m/K) has long been hypothesized to be associated with a duricrust. The fraction of cement required to fit the thermal data is less than approx.1-5% by volume. This small amount of material is consistent with orbital observations, confirming that soil cementation is an important factor controlling the thermal inertia of the Martian surface

Piqueux, S.↗
NASA NTRS2011

Direct Simulation of Multiple Scattering by Discrete Random Media Illuminated by Gaussian Beams

The conventional orientation-averaging procedure developed in the framework of the superposition T-matrix approach is generalized to include the case of illumination by a Gaussian beam (GB). The resulting computer code is parallelized and used to perform extensive numerically exact calculations of electromagnetic scattering by volumes of discrete random medium consisting of monodisperse spherical particles. The size parameters of the scattering volumes are 40, 50, and 60, while their packing density is fixed at 5%. We demonstrate that all scattering patterns observed in the far-field zone of a random multisphere target and their evolution with decreasing width of the incident GB can be interpreted in terms of idealized theoretical concepts such as forward-scattering interference, coherent backscattering (CB), and diffuse multiple scattering. It is shown that the increasing violation of electromagnetic reciprocity with decreasing GB width suppresses and eventually eradicates all observable manifestations of CB. This result supplements the previous demonstration of the effects of broken reciprocity in the case of magneto-optically active particles subjected to an external magnetic field.

Mackowski, Daniel W.↗
NASA NTRS2011

Cancer Risk Estimates from Space Flight Estimated Using Yields of Chromosome Damage in Astronaut's Blood Lymphocytes

To date, cytogenetic damage has been assessed in blood lymphocytes from more than 30 astronauts before and after they participated in long-duration space missions of three months or more on board the International Space Station. Chromosome damage was assessed using fluorescence in situ hybridization whole chromosome analysis techniques. For all individuals, the frequency of chromosome damage measured within a month of return from space was higher than their preflight yield, and biodosimetry estimates were within the range expected from physical dosimetry. Follow up analyses have been performed on most of the astronauts at intervals ranging from around 6 months to many years after flight, and the cytogenetic effects of repeat long-duration missions have so far been assessed in four individuals. Chromosomal aberrations in peripheral blood lymphocytes have been validated as biomarkers of cancer risk and cytogenetic damage can therefore be used to characterize excess health risk incurred by individual crewmembers after their respective missions. Traditional risk assessment models are based on epidemiological data obtained on Earth in cohorts exposed predominantly to acute doses of gamma-rays, and the extrapolation to the space environment is highly problematic, involving very large uncertainties. Cytogenetic damage could play a key role in reducing uncertainty in risk estimation because it is incurred directly in the space environment, using specimens from the astronauts themselves. Relative cancer risks were estimated from the biodosimetry data using the quantitative approach derived from the European Study Group on Cytogenetic Biomarkers and Health database. Astronauts were categorized into low, medium, or high tertiles according to their yield of chromosome damage. Age adjusted tertile rankings were used to estimate cancer risk and results were compared with values obtained using traditional modeling approaches. Individual tertile rankings increased after space flight and analysis of follow up samples indicated that the tertile rankings remained in the high category for more than 50% of the individuals assessed so far. Crewmembers that shift and remain in the high category are projected to have increased life-time cancer risk.

George, Kerry A.↗
NASA NTRSDate not supplied

Simulation of the Rotorwash Induced by a Quadrotor Urban Air Taxi in Ground Effect

A rotorcraft hovering near the ground causes downwash and outwash. The impact of these induced velocities on urban air mobility operations has not been extensively quantified. This paper explores the opportunity of using CFD to perform dedicated studies on the aerodynamics of rotorcraft in ground effect (IGE). For this purpose, we compare load and flow predictions obtained with the high-fidelity CFD solver OVERFLOW and with a medium-fidelity vortex particle-mesh (VPM) method, for a single rotor IGE. We show that the computational cost of high-fidelity simulation makes it impractical to sweep through a large number of configurations or operating conditions. However, a small set of cases can be used to verify a medium-fidelity tool. The latter provides a better trade-off between “accuracy” and computational intensity, which enables numerous, longer simulations at a more affordable cost. As an example, the outwash flow is computed for two different designs of a quadrotor air taxi in hover and reveals the existence of increased velocities between the rotors. This work illustrates how CFD can help identify dangerous areas for passengers and ground personnel when they approach the vehicle.

HECC↗
NASA NTRS2015

Determination of the Contact Angle Based on the Casimir Effect

In several crystal growth processed based on capillarity, a melt comes into contact with a crucible wall at an angle defined as the contact angle. For molten metals and semiconductors, this contact angle is dependent upon both the crucible and melt material and typical values fall in the range 80-170deg. However, on a microscopic scale, there does not exist a precise and sharp contact angle but rather the melt and solid surfaces merge smoothly and continuously over a distance of up to several micrometers. Accurate modeling requires a more advanced treatment of this interaction. The interaction between the melt and solid surfaces can be calculated by considering two forces: a short-range repulsive force and a longer range (up to a few micrometers) Casimir force. The Casimir force between the two bodies of complex geometry is calculated using a retarded temperature Green's function (Matsubara type) for the photon in the medium. The governing equations are cast in the form of a set of boundary integral equations which are then solved numerically for the case of molten Ge on SiO2. The shape of the molten surface approaching the flat solid body is determined, and the contact angle is defined as the angle between the two surfaces at the microscopically asymptotic distance of 1-2 micrometers. The formulation of this model and the results of the numerical calculations will be presented and discussed.

Mazuruk, K.↗
NASA NTRS2000

Crack Turning in Integrally Stiffened Aircraft Structures

Current emphasis in the aircraft industry toward reducing manufacturing cost has created a renewed interest in integrally stiffened structures. Crack turning has been identified as an approach to improve the damage tolerance and fail-safety of this class of structures. A desired behavior is for skin cracks to turn before reaching a stiffener, instead of growing straight through. A crack in a pressurized fuselage encounters high T-stress as it nears the stiffener--a condition favorable to crack turning. Also, the tear resistance of aluminum alloys typically varies with crack orientation, a form of anisotropy that can influence the crack path. The present work addresses these issues with a study of crack turning in two-dimensions, including the effects of both T-stress and fracture anisotropy. Both effects are shown to have relation to the process zone size, an interaction that is central to this study. Following an introduction to the problem, the T-stress effect is studied for a slightly curved semi-infinite crack with a cohesive process zone, yielding a closed form expression for the future crack path in an infinite medium. For a given initial crack tip curvature and tensile T-stress, the crack path instability is found to increase with process zone size. Fracture orthotropy is treated using a simple function to interpolate between the two principal fracture resistance values in two-dimensions. An extension to three-dimensions interpolates between the six principal values of fracture resistance. Also discussed is the transition between mode I and mode II fracture in metals. For isotropic materials, there is evidence that the crack seeks out a direction of either local symmetry (pure mode I) or local asymmetry (pure mode II) growth. For orthotropic materials the favored states are not pure modal, and have mode mixity that is a function of crack orientation.

Pettit, Richard Glen↗
NASA NTRS2011

Biological Based Risk Assessment for Space Exploration

Exposures from galactic cosmic rays (GCR) - made up of high-energy protons and high-energy and charge (HZE) nuclei, and solar particle events (SPEs) - comprised largely of low- to medium-energy protons are the primary health concern for astronauts for long-term space missions. Experimental studies have shown that HZE nuclei produce both qualitative and quantitative differences in biological effects compared to terrestrial radiation, making risk assessments for cancer and degenerative risks, such as central nervous system effects and heart disease, highly uncertain. The goal for space radiation protection at NASA is to be able to reduce the uncertainties in risk assessments for Mars exploration to be small enough to ensure acceptable levels of risks are not exceeded and to adequately assess the efficacy of mitigation measures such as shielding or biological countermeasures. We review the recent BEIR VII and UNSCEAR-2006 models of cancer risks and their uncertainties. These models are shown to have an inherent 2-fold uncertainty as defined by ratio of the 95% percent confidence level to the mean projection, even before radiation quality is considered. In order to overcome the uncertainties in these models, new approaches to risk assessment are warranted. We consider new computational biology approaches to modeling cancer risks. A basic program of research that includes stochastic descriptions of the physics and chemistry of radiation tracks and biochemistry of metabolic pathways, to emerging biological understanding of cellular and tissue modifications leading to cancer is described.

Cucinotta, Francis A.↗
NASA NTRS2003

The Conference Proceedings of the 2003 Air Transport Research Society (ATRS) World Conference, Volume 5

The UNO Aviation Institute Monograph Series began in 1994 as a key component of the education outreach and information transfer missions of the Aviation Institute and the NASA Nebraska Space Grant & EPSCoR Programs. The series is an outlet for aviation materials to be indexed and disseminated through an efficient medium. Publications are welcome in all aspects of aviation. Publication formats may include, but are not limited to, conference proceedings, bibliographies, research reports, manuals, technical reports, and other documents that should be archived and indexed for future reference by the aviation and world wide communities. The Conference proceedings of the 2003 Air Transport Research Society (ATRS) world conference, volume 5 is presented. The topics include: 1) The Temporal Configuration of Airline Networks in Europe; 2) Determination and Applications of Environmental Costs at Different Sized Airports-Aircraft Noise and Engine Emissions; 3) Cost Effective Measures to Reduce CO2 Emissions in the Air Freight Sector; 4) An Assessment of the Sustainability of Air Transport System: Quantification of Indicators; 5) Regulation, Competition and Network Evolution in Aviation; 6) Regulation in the Air: Price and Frequency Cap; 7) Industry Consolidation and Future Airline Network Structures in Europe; 8) Application of Core Theory to the U.S. Airline Industry; 9) Air Freight Transshipment Route Choice Analysis; 10) A Fuzzy Approach of the Competition on Air Transport Market; and 11) Developing Passenger Demand Models for International Aviation from/to Egypt: A Case Study of Cairo Airport and Egyptair.

Bowen, Brent↗
NASA NTRS1995

Cell shape, cytoskeletal mechanics, and cell cycle control in angiogenesis

Capillary endothelial cells can be switched between growth and differentiation by altering cell-extracellular matrix interactions and thereby, modulating cell shape. Studies were carried out to determine when cell shape exerts its growth-regulatory influence during cell cycle progression and to explore the role of cytoskeletal structure and mechanics in this control mechanism. When G0-synchronized cells were cultured in basic fibroblast growth factor (FGF)-containing defined medium on dishes coated with increasing densities of fibronectin or a synthetic integrin ligand (RGD-containing peptide), cell spreading, nuclear extension, and DNA synthesis all increased in parallel. To determine the minimum time cells must be adherent and spread on extracellular matrix (ECM) to gain entry into S phase, cells were removed with trypsin or induced to retract using cytochalasin D at different times after plating. Both approaches revealed that cells must remain extended for approximately 12-15 h and hence, most of G1, in order to enter S phase. After this restriction point was passed, normally 'anchorage-dependent' endothelial cells turned on DNA synthesis even when round and in suspension. The importance of actin-containing microfilaments in shape-dependent growth control was confirmed by culturing cells in the presence of cytochalasin D (25-1000 ng ml-1): dose-dependent inhibition of cell spreading, nuclear extension, and DNA synthesis resulted. In contrast, induction of microtubule disassembly using nocodazole had little effect on cell or nuclear spreading and only partially inhibited DNA synthesis. Interestingly, combination of nocodazole with a suboptimal dose of cytochalasin D (100 ng ml-1) resulted in potent inhibition of both spreading and growth, suggesting that microtubules are redundant structural elements which can provide critical load-bearing functions when microfilaments are partially compromised. Similar synergism between nocodazole and cytochalasin D was observed when cytoskeletal stiffness was measured directly in living cells using magnetic twisting cytometry. These results emphasize the importance of matrix-dependent changes in cell and nuclear shape as well as higher order structural interactions between different cytoskeletal filament systems for control of capillary cell growth during angiogenesis.

Non-NASA Center↗