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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 127 records · Page 7

Data for "RT-EZ: A Golden Gate Assembly Toolkit for Streamlined Genetic Engineering of Rhodotorula toruloides"

For economic and sustainable biomanufacturing, the oleaginous yeast Rhodotorula toruloides has emerged as a promising platform for producing biofuels, pharmaceuticals, and other valuable chemicals. However, genetic manipulation of R. toruloides has been limited by its high GC content and the lack of a replicating plasmid, necessitating gene integration into the genome of the yeast. To address these challenges, we developed the RT-EZ ( R. toruloides Efficient Zipper) toolkit, a versatile tool based on Golden Gate assembly, designed to streamline R. toruloides engineering with improved efficiency and flexibility. The RT-EZ toolkit simplifies vector construction by incorporating new features such as bidirectional promoters and 2A peptides, color-based screening using RFP, and sequences optimized for both Agrobacterium tumefaciens-mediated transformation (ATMT) and easy linearization, enabling straightforward selection and transformation. Notably, the RT-EZ kit can be used to construct an expression cassette with four different genes in one assembly reaction, significantly improving vector construction speed and efficiency. The utility of the RT-EZ toolkit was demonstrated through the successful synthesis of arachidonic acid in R. toruloides by coexpressing fatty acid elongases and desaturases. This result underscores the potential of the RT-EZ toolkit to advance synthetic biology in R. toruloides , providing a streamlined method for addressing genetic engineering challenges in the yeast.

gene editing↗

A Geodatabase Designed to Inform and Support Safe CO2 Transport-Route Planning

The National Energy Technology Laboratory developed the Carbon Capture and Storage (CCS) Pipeline Route Planning Database to inform safe and sustainable CO2 transport planning in support of decarbonization efforts. This comprehensive Esri Geodatabase contains over 90 GBs of data and 60+ spatial layers representing key considerations including natural hazards, infrastructure, energy, and social justice. Leveraging ArcGIS Pro, nationwide raster, and vector datasets containing millions of features were processed to be easily digestible for users and complex modeling software.

Romeo, Lucy↗

TCR-H: explainable machine learning prediction of T-cell receptor epitope binding on unseen datasets

Artificial-intelligence and machine-learning (AI/ML) approaches to predicting T-cell receptor (TCR)-epitope specificity achieve high performance metrics on test datasets which include sequences that are also part of the training set but fail to generalize to test sets consisting of epitopes and TCRs that are absent from the training set, i.e., are ‘unseen’ during training of the ML model. We present TCR-H, a supervised classification Support Vector Machines model using physicochemical features trained on the largest dataset available to date using only experimentally validated non-binders as negative datapoints. TCR-H exhibits an area under the curve of the receiver-operator characteristic (AUC of ROC) of 0.87 for epitope ‘hard splitting’ (i.e., on test sets with all epitopes unseen during ML training), 0.92 for TCR hard splitting and 0.89 for ‘strict splitting’ in which neither the epitopes nor the TCRs in the test set are seen in the training data. Furthermore, we employ the SHAP (Shapley additive explanations) eXplainable AI (XAI) method for post hoc interrogation to interpret the models trained with different hard splits, shedding light on the key physiochemical features driving model predictions. TCR-H thus represents a significant step towards general applicability and explainability of epitope:TCR specificity prediction.

60 APPLIED LIFE SCIENCES↗

Low-speed wind-tunnel test of a STOL supersonic-cruise fighter concept

A wind-tunnel investigation was conducted to examine the low-speed static stability and control characteristics of a 0.10 scale model of a STOL supersonic cruise fighter concept. The concept, referred to as a twin boom fighter, was designed as a STOL aircraft capable of efficient long range supersonic cruise. The configuration name is derived from the long twin booms extending aft of the engine to the twin vertical tails which support a high center horizontal tail. The propulsion system features a two dimensional thrust vectoring exhaust nozzle which is located so that the nozzle hinge line is near the aircraft center of gravity. This arrangement is intended to allow large thrust vector angles to be used to obtain significant values of powered lift, while minimizing pitching moment trim changes. Low speed stability and control information was obtained over an angle of attack range including the stall. A study of jet induced power effects was included.

Coe, Paul L., Jr.↗

Computational Methods for Frictional Contact With Applications to the Space Shuttle Orbiter Nose-Gear Tire

A computational procedure is presented for the solution of frictional contact problems for aircraft tires. A Space Shuttle nose-gear tire is modeled using a two-dimensional laminated anisotropic shell theory which includes the effects of variations in material and geometric parameters, transverse-shear deformation, and geometric nonlinearities. Contact conditions are incorporated into the formulation by using a perturbed Lagrangian approach with the fundamental unknowns consisting of the stress resultants, the generalized displacements, and the Lagrange multipliers associated with both contact and friction conditions. The contact-friction algorithm is based on a modified Coulomb friction law. A modified two-field, mixed-variational principle is used to obtain elemental arrays. This modification consists of augmenting the functional of that principle by two terms: the Lagrange multiplier vector associated with normal and tangential node contact-load intensities and a regularization term that is quadratic in the Lagrange multiplier vector. These capabilities and computational features are incorporated into an in-house computer code. Experimental measurements were taken to define the response of the Space Shuttle nose-gear tire to inflation-pressure loads and to inflation-pressure loads combined with normal static loads against a rigid flat plate. These experimental results describe the meridional growth of the tire cross section caused by inflation loading, the static load-deflection characteristics of the tire, the geometry of the tire footprint under static loading conditions, and the normal and tangential load-intensity distributions in the tire footprint for the various static vertical loading conditions. Numerical results were obtained for the Space Shuttle nose-gear tire subjected to inflation pressure loads and combined inflation pressure and contact loads against a rigid flat plate. The experimental measurements and the numerical results are compared.

Tanner, John A.↗

V/STOL systems research aircraft: A tool for cockpit integration

The next generation ASTOVL aircraft will have a complicated propulsion system. The configuration choices include Direct Lift, Lift-Fan and Lift + Lift/Cruise but the aircraft must also have supersonic performance and low-observable characteristics. The propulsion system may have features such as flow blockers, vectoring nozzles and flow transfer schemes. The flight control system will necessarily fully integrate the aerodynamic surfaces and the propulsive elements. With a fully integrated, fly-by-wire flight/propulsion control system, the options for cockpit integration are interesting and varied. It is possible to de-couple longitudinal and vertical responses allowing the pilot to close the loop on flightpath and flightpath acceleration directly. In the hover, the pilot can control the translational rate directly without having to stabilize the inner rate and attitude loops. The benefit of this approach, reduced workload and increased precision, has previously been demonstrated through several motion-based simulations. In order to prove the results in flight, the V/STOL System Research Aircraft (VSRA) was developed at the NASA Ames Research Center. The VSRA is the YAV-8B Prototype modified with a research flight control system using a series-parallel servo configuration in all the longitudinal degrees of freedom (including thrust and thrust vector angle) to provide an integrated flight and propulsion control system in a limited envelope. Development of the system has been completed and flight evaluations of the response types have been performed. In this paper we will discuss the development of the VSRA, the evolution of the flightpath command and translational rate command response types and the Guest Pilot evaluations of the system. Pilot evaluation results are used to draw conclusions regarding the suitability of the system to satisfy V/STOL requirements.

Stortz, Michael W.↗

Computational methods for frictional contact with applications to the Space Shuttle orbiter nose-gear tire: Comparisons of experimental measurements and analytical predictions

A computational procedure is presented for the solution of frictional contact problems for aircraft tires. A Space Shuttle nose-gear tire is modeled using a two-dimensional laminated anisotropic shell theory which includes the effects of variations in material and geometric parameters, transverse-shear deformation, and geometric nonlinearities. Contact conditions are incorporated into the formulation by using a perturbed Lagrangian approach with the fundamental unknowns consisting of the stress resultants, the generalized displacements, and the Lagrange multipliers associated with both contact and friction conditions. The contact-friction algorithm is based on a modified Coulomb friction law. A modified two-field, mixed-variational principle is used to obtain elemental arrays. This modification consists of augmenting the functional of that principle by two terms: the Lagrange multiplier vector associated with normal and tangential node contact-load intensities and a regularization term that is quadratic in the Lagrange multiplier vector. These capabilities and computational features are incorporated into an in-house computer code. Experimental measurements were taken to define the response of the Space Shuttle nose-gear tire to inflation-pressure loads and to inflation-pressure loads combined with normal static loads against a rigid flat plate. These experimental results describe the meridional growth of the tire cross section caused by inflation loading, the static load-deflection characteristics of the tire, the geometry of the tire footprint under static loading conditions, and the normal and tangential load-intensity distributions in the tire footprint for the various static vertical-loading conditions. Numerical results were obtained for the Space Shuttle nose-gear tire subjected to inflation pressure loads and combined inflation pressure and contact loads against a rigid flat plate. The experimental measurements and the numerical results are compared.

Tanner, John A.↗

V/STOL Systems Research Aircraft: A Tool for Cockpit Integration

The next generation ASTOVL aircraft will have a complicated propulsion System. The configuration choices include Direct Lift, Lift-Fan and Lift+Lift /Cruise but the aircraft must also have supersonic performance and low-observable characteristics. The propulsion system may have features such as flow blockers, vectoring nozzles and flow transfer schemes. The flight control system will necessarily fully integrate the aerodynamic surfaces and the propulsive elements. With a fully integrated, fly-by-wire flight/propulsion control system, the options for cockpit integration are interesting and varied. It is possible to decouple longitudinal and vertical responses allowing the pilot to close the loop on flight path and flight path acceleration directly. In the hover, the pilot can control the translational rate directly without having to stabilize the inner rate and attitude loops. The benefit of this approach, reduced workload and increased precision. has previously been demonstrated through several motion-based simulations. In order to prove the results in flight, the V/STOL System Research Aircraft (VSRA) was developed at the NASA Ames Research Center. The VSRA is the YAV-8B Prototype modified with a research flight control system using a series-parallel servo configuration in all the longitudinal degrees of freedom (including thrust and thrust vector angle) to provide an integrated flight and propulsion control system in a limited envelope. Development of the system has been completed and flight evaluations of the response types have been performed. In this paper we will discuss the development of the VSRA, the evolution of the flight path command and translational rate command response types and the Guest Pilot evaluations of the system. Pilot evaluation results will be used to draw conclusions regarding the suitability of the system to satisfy V/STOL requirements.

Stortz, Michael W.↗

Array Transposition in SSD

One obstacle to running very large two- and three-dimensional codes on the Cray X-MP and Y-MP systems is to efficiently perform array transpositions using SSD storage. This article discusses how such transpositions can be performed by means of algorithms that feature exclusively unit stride, long vector transfers between main memory and SSD, and which only require a single pass through the data (provided sufficient main memory buffers are available).

Bailey, David H.↗

Array Transposition in SSD

One obstacle to running very large two- and three-dimensional codes on the Cray X-MP and Y-MP systems is to efficiently perform array transpositions using SSD storage. This article discusses how such transpositions can be performed by means of algorithms that feature exclusively unit stride, long vector transfers between main memory and SSD, and which only require a single pass through the data (provided sufficient main memory buffers are available).

Bailey, David H.↗

Portable Laser Guided Robotic Metrology (PLGRM) System

This paper introduces the new Portable Laser Guided Robotic Metrology (PLGRM) system at the National Aeronautics and Space Administration's (NASA) Glenn Research Center. Previous work used industrial robots in fixed facilities to characterize antennas and required fixtures that do not lend themselves to portable applications. NASA's PLGRM system is designed for in-situ antenna measurements at a remote site.The system consists of a collaborative robot arm mounted on a vertical lift and a laser tracker, each on a mobile base. Together,they enable scanning a surface larger than the robot's reach.To accomplish this, the robot first collects all points within its reach, then the system is moved and the laser tracker is used to relocate the robot before additional points are captured. The PLGRM implementation will be discussed including how safety and planning are combined to effectively characterize antennas.Software defined triggering is a feature, for flexible integration of vector network analyzers and antenna controllers. Lastly, data will be shown to demonstrate system functionality and accuracy.

Slater, Peter A.↗

Geostar – a ‘Shovel Ready’ Geostationary Microwave Sounder

A geostationary microwave sounder, capable of providing temperature, water vapor, clouds, precipitation, and wind in the presence of clouds, convection and precipitation, will add tremendously to our ability to observe dynamic atmospheric phenomena, such as hurricanes and severe storms, monsoonal moisture flow, atmospheric rivers, etc. Such a sensor is now feasible, enabled by technology that has been developed under NASA’s Instrument Incubator Program at the Jet Propulsion Laboratory. The Geostationary Synthetic Thinned Aperture Radiometer (GeoSTAR) is essentially an “AMSU in GEO”, i.e. it has similar capabilities as AMSU would have if it were operating on a geostationary satellite, but with similar spatial resolution. GEO orbits are almost 50 times higher than the LEO orbits that current AMSUs operate from, and the corresponding scaling of aperture size required to maintain spatial resolution has stymied the development of such a sensor for many decades. The aperture synthesis approach implemented with GeoSTAR finally overcomes that obstacle, and the large number of microwave receivers and associated electronics required is made possible with new technology that has now been developed. A low-cost mission operating as a hosted payload on a commercial communications satellite has been designed and is “shovel-ready”. The objectives of such a mission could be to advance our understanding and modeling of storm processes that control rapid or explosive intensification of hurricanes, mesoscale convective systems, and extratropical cyclones, or it could be to support operational weather forecasting and nowcasting. Retrieval of temperature and water vapor profiles is possible even in the presence of moderate precipitation, and with the ability to derive horizontal wind vectors by tracking water vapor features, a rich set of parameters measuring the thermodynamics, dynamics and convection and sampled every 15 minutes, will enable significant progress in storm science and forecasting.

Lambrigtsen, Bjorn H↗

Video data compression using artificial neural network differential vector quantization

An artificial neural network vector quantizer is developed for use in data compression applications such as Digital Video. Differential Vector Quantization is used to preserve edge features, and a new adaptive algorithm, known as Frequency-Sensitive Competitive Learning, is used to develop the vector quantizer codebook. To develop real time performance, a custom Very Large Scale Integration Application Specific Integrated Circuit (VLSI ASIC) is being developed to realize the associative memory functions needed in the vector quantization algorithm. By using vector quantization, the need for Huffman coding can be eliminated, resulting in superior performance against channel bit errors than methods that use variable length codes.

Krishnamurthy, Ashok K.↗

Minimum impulse three-body trajectories.

A rapid and accurate method of calculating optimal impulsive transfers in the restricted problem of three bodies has been developed. The technique combines a multi-conic method of trajectory integration with primer vector theory and an accelerated gradient method of trajectory optimization. A unique feature is that the state transition matrix and the primer vector are found analytical without additional integrations or differentiations. The method has been applied to the determination of optimal two and three impulse transfers between the L2 libration point and circular orbits about both the earth and the moon.

D'Amario, L.↗

A new radar determination of the spin vector of Venus

Two radar observations of a set of three relatively small features on the surface of Venus have facilitated a refined determination of the spin vector of Venus. The period is found to be 243.019 + or 0.014 days, while the obliquity is 177.22 + or - 0.18 deg. The effects of deviations from exact sphericity on the interpretation of the measurements are discussed at length and the question of resonance with earth is reexamined.

Zohar, S.↗

Polarimetric signatures of a layer of random nonspherical discrete scatterers overlying a homogeneous half-space based on first- and second-order vector radiative transfer theory

Complete polarimetric signatures of a layer of random, nonspherical discrete scatterers overlying a homogeneous half space are studied with the first- and second-order solutions of the vector radiative transfer theory. Some of the salient features of the numerical results are as follows: (1) the inclusion of the nondiagonal extinction matrix in the vector radiative transfer theory accounts for an appreciable phase difference between vv and hh polarizations, particularly for aligned scatterers; (2) the ensemble-averaged scattered Stokes vector is generally partially polarized, with the degree of polarization less than unity; (3) there generally exists a pedestal in the copolarization return when plotted as a function of ellipticity and orientation angles, which may be due to heterogeneity of scattering objects and/or multiple scattering effects; and (4) multiple scattering effects generally enhance the pedestal in copolarization return, decrease the degree of polarization, affect phase difference, and also enhance the depolarization return.

Tsang, Leung↗

Nonstationary Dynamics Data Analysis with Wavelet-SVD Filtering

Nonstationary time-frequency analysis is used for identification and classification of aeroelastic and aeroservoelastic dynamics. Time-frequency multiscale wavelet processing generates discrete energy density distributions. The distributions are processed using the singular value decomposition (SVD). Discrete density functions derived from the SVD generate moments that detect the principal features in the data. The SVD standard basis vectors are applied and then compared with a transformed-SVD, or TSVD, which reduces the number of features into more compact energy density concentrations. Finally, from the feature extraction, wavelet-based modal parameter estimation is applied.

Brenner, Marty↗