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Unicellular microorganisms size and concentration estimates based on light scattering measurements of suspensions in spectrophotometer cuvette compartment
Power spectrum of light scattered by two level atom driven by monochromatic electric field obtained from atomic dipole moment correlation function
A hybrid method for light scattering by an arbitrary particle using the scattering-order formulation of the coupled dipole method is described. An arbitrary particle is divided into two or more segments, and the field scattered by the particle is obtained from the fields scattered by each segment together with the field due to interactions among segments. An exact or approximate theory is used to calculate the scattered field from each segment, and interactions are included using the scattering-order formulation of the coupled dipole method. Calculations show that for certain particles, this hybrid approach can require fewer computations and give more accurate results than the scattering-order method.
The development of a versatile, miniature, modular light scattering instrument to be used in microgravity is described. The instrument will measure microscopic particles in the size range of thirty angstroms to above three microns. This modular instrument permits several configurations, each optimized for a particular experiment. In particular, a multiangle instrument will probably be mounted in a rack in the Space Shuttle and on the Space Station. It is possible that a Space Shuttle glove-box and a lap-top computer containing a correlator card can be used to perform a number of experiments and to demonstrate the technology needed for more elaborate investigations.
We have performed multiangle static and dynamic light scattering studies of lysozyme solutions at pH=4.7. The Rayleigh ratio R(sub g) and the collective diffusion coefficient D(sub c) were determined as function of both protein concentration c(sub p) and salt concentration c(sub s) with two different salts. At low salt concentrations, the scattering ratio K(sub c)(sub p)/R(sub theta) and diffusivity increased with protein concentration above the values for a monomeric, ideal solution. With increasing salt concentration this trend was eventually reversed. The hydrodynamic interactions of lysozyme in solution, extracted from the combination of static and dynamic scattering data, decreased significantly with increasing salt concentration. These observations reflect changes in protein interactions, in response to increased salt screening, from net repulsion to net attraction. Both salts had the same qualitative effect, but the quantitative behavior did not scale with the ionic strength of the solution. This indicates the presence of salt specific effects. At low protein concentrations, the slopes of K(sub c)(sub p)/R(sub theta) and D(sub c) vs c(sub p) were obtained. The dependence of the slopes on ionic strength was modeled using a DLVO potential for colloidal interactions of two spheres, with the net protein charge Z(sub e) and Hamaker constant A(sub H) as fitting parameters. The model reproduces the observed variations with ionic strength quite well. Independent fits to the static and dynamic data, however, led to different values of the fitting parameters. These and other shortcomings suggest that colloidal interaction models alone are insufficient to explain protein interactions in solutions.
The 14th Electromagnetic and Light Scattering Conference (ELS-XIV) was held at the Universit de Lille 1, Villeneuve d'Ascq, France on 17-21 June 2013. The conference was attended by 200 scientists from 26 countries. The scientific program included one plenary lecture, 12 invited reviews, 100 contributed oral talks, and 86 poster presentations. The program, the abstracts, and the slides of the oral presentations are available at the conference web site http:www-loa.univ-lille1.frELS-XIV. To highlight one of the traditional ELS themes, the ELS-XIV featured a special session on Remote sensing of aerosols and clouds using polarimetric observations. This session was sponsored and co-organized by the French space agency CNES and attracted representatives from nearly all research teams word-wide involved in the development and active use of space-borne, in situ, and ground-based polarimetric observations.
Mechanisms of light scattering in concentrated pigment films and experiments in optical properties of silver halide particle arrays
The 15th Electromagnetic and Light Scattering Conference (ELS-XV) was held in Leipzig, Germany from 21 to 26 of June 2015. This conference built on the great success of the previous meetings held in Amsterdam (1995), Helsinki(1997) [2], New York City(1998) [3], Vigo (1999),Halifax (2000), Gainesville (2002), Bremen (2003), Salobreña (2005), St. Petersburg (2006), Bodrum (2007), Hatfield (2008), Helsinki (2010), Taormina (2011), and Lille as well as the workshops held in Bremen (1996,1998) and Moscow (1997). As usual, the main objective of this conference was to bring together scientists, engineers, and PhD students studying various aspects of electromagnetic scattering and to provide a relaxed atmosphere for in-depth discussion of theory, measurements, and applications. Furthermore, ELS-XV supported the United Nations "Year of Light" and celebrated the150th anniversary of Maxwell's electromagnetics. Maxwell's paper on "A Dynamical Theory of the Electromagnetic Field" was published in1865 and has widely been acknowledged as one of the supreme achievements in the history of science. The conference was attended by136 scientists from 22 countries. The scientific program included two plenary lectures, 16 invited reviews, 88 contributed oral talks, and 70 poster presentations. The program and the abstracts of conference presentations are available at the conference website http://www.els-xv-2015.net/home.html. Following the well-established ELS practice and with Elsevier's encouragement, we solicited full-size papers for a topical issue of the Journal of Quantitative Spectroscopy and Radiative Transfer (JQSRT). The result of this collective effort is now in the reader's hands. As always, every invited review and regular paper included in this topical issue has undergone the same rigorous peer review process as any other manuscript published in the JQSRT.
A variety of studies were carried out to help establish the accuracy of quantities describing physical characteristics of cloud particles (such as size, shape, and composition) that are to be inferred from light scattering data obtained with the nephelameter experiment on the Galileo spacecraft. The objectives were to provide data for validating and testing procedures for analyzing the Galileo nephelameter data with light scattering observations in a variety of on-going laboratory and field measurement programs for which simultaneous observations of the physical characteristics of the scattering particles were available.
NASA is studying the properties of mirror surfaces for X-ray telescopes, the data of which will be used to develop the telescope system for the Advanced X-ray Astronomical Facility. Visible light scatter measurements, using a computer controlled scanner, are made of various mirror samples to determine surface roughness. Total diffuse scatter is calculated using numerical integration techniques and used to estimate the rms surface roughness. The data measurements are then compared with X-ray scatter measurements of the same samples. A summary of the data generated is presented, along with graphs showing changes in scatter on samples before and after cleaning. Results show that very smooth surfaces can be polished on the common substrate materials (from 2 to 10 Angstroms), and nickel appears to give the lowest visible light scatter.
Instrument measures concentration of small particles in aqueous medium in terms of amount of light scattered and degree to which light transmission is attenuated. Sensitivity to small particles is optimized because both scattered and transmitted illumination levels are detected by photodiodes.
Efficient production of a wide range of commercial products based on submicron colloidal dispersions would benefit from instrumentation for online particle sizing, permitting real time monitoring and control of the particle size distribution. Recent advances in the technology of dynamic light scattering (DLS), especially improvements in algorithms for inversion of the intensity autocorrelation function, have made it ideally suited to the measurement of simple particle size distributions in the difficult submicron region. Crucial to the success of an online DSL based instrument is a simple mechanism for automatically sampling and diluting the starting concentrated sample suspension, yielding a final concentration which is optimal for the light scattering measurement. A proprietary method and apparatus was developed for performing this function, designed to be used with a DLS based particle sizing instrument. A PC/AT computer is used as a smart controller for the valves in the sampler diluter, as well as an input-output communicator, video display and data storage device. Quantitative results are presented for a latex suspension and an oil-in-water emulsion.
Radiance and polarization of multiple scattered light from haze and clouds
Radiance and polarization of multiple scattered light from haze and clouds calculated from Stokes vectors by Monte Carlo method
In the present paper, the light scattering characteristics of spheroidal particles are evaluated within the framework of a scattering theory developed for a homogeneous isotropic spheroid. This approach is shown to be well suited for computing the scattering quantities of spheroidal particles of fairly large sizes (up to a size parameter of 30). The effects of particle size, shape, index of refraction, and orientation on the scattering efficiency factors and the scattering intensity functions are studied and interpreted physically. It is shown that, in the case of oblique incidence, the scattering properties of a long slender prolate spheroid resemble those of an infinitely long circular cylinder.
A study of the light scattering properties of randomly oriented, identical spheroidal particles is presented. A computation method was developed to integrate the Asano and Yamomoto solution (1975) for scattering from a homogeneous spheroid over all particle orientations; the extinction and scattering cross-sections, the asymmetry factor, and scattering matrix elements are calculated for randomly oriented prolate and oblate spheroids and compared with the calculations for spheres and laboratory measurements. The angular scattering behavior of spheroids is found to be different from that of the spheres for side scattering to backscattering directions, and prolate and oblate spheroids of the same shape parameter have similar angular scattering patterns.
Monte Carlo simulation techniques applied to light scattering in highly reflective pigmented coatings
Measurement of laser beam light scattered by atmosphere