An improved technique for microbiological sampling of surfaces - Agar spray Research report, period ending 30 Sep. 1968
Improved agar spray technique for sampling surface microorganism
SEARCH · Search NASA
Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.
Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.
Improved agar spray technique for sampling surface microorganism
Laminar air flow cabinets for controlling microbial contamination in tissue cultures preparation
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Report deals with an investigation of vertical laminar flow clean rooms for use in spacecraft assembly. A reduction of particulate and microbial contamination occurs in the application.
Experimental studies on behavior and survival of Bacillus subtilis var. niger spores and microbial detection as related to planetary quarantine
Statistical models for spacecraft sterilization and microbial growths
Survival of bacterial spores under various temperature and humidity conditions related to planetary quarantine
Subjects on planetary flight missions, supporting technology, microbial release probabilities, heat sterilization cycles
Statistical procedures for investigating decontamination of spacecraft and microbial assays
Hydrocarbon distribution of various algae and bacteria, discussing hydrocarbons diagenesis and biological transformations in sediments
Penicillium mutant chemical stress tolerance in boric acid and potassium chloride selective media, studying carbohydrate and inosine-5-phosphate effects on growth rate
Simple, low cost system consists of semirigid incubation chamber with clear Teflon window, airtight zipper and internal compartments for petri dishes or microbial plates. Device interests schools, medical laboratories, and manufacturers of biological and pharmaceutical supplies.
Effects of dry heat and chemicals on long term survival rates of bacteria spores under varying temperatures and humidity conditions
Mobile sampling cart automatically swabs surfaces for the recovery of microorganisms. Unit operates without human involvement and provides for control of swabbing speed, rotation of cotton swab, and the pressure and angle applied to swab. Capability of reverse direction is also available. Sampling cart use is limited to flat surfaces.
The experiments carried out to determine the effects of temperature and relative humidity on the survival rate of Bacillus subtillis var. niger spores are reported. The experiments were conducted in environmental chambers at temperatures of 75 and 90 C. Data are also included on the survival characteristics of the spores suspended in sucrose solutions at 90 C with water activities of 0.99, 0.9, and 0.85
The results of studies to determine the effect of soil particle size on the survival time at 125 C of the microflora associated with these particles are discussed. The data suggest that longer survival times exist for the microflora associated with larger particles. The studies indicate that microorganisms associated with soil are difficult to kill and that organisms associated with large particles are harder to kill than those associated with small particles. Sterlization requirements increase as the level of contamination increases. Soil particles and their accompanying microflora are the most critical contaminants.
The survival of Bacillus subtilis var. niger spores suspended in solutions of sucrose and glycerol at calculated water activities and varying temperatures was studied. The overall results indicated that as the water activity of the liquid decreased from .99 to .85, the heat resistance of the spores increased. The nature of the substance controlling the water activity, and the history of the spores prior to treatment also had an affect on their heat resistance.