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At least 127 records · Page 7

A Telomerase-Activated Magnetic Resonance Imaging Probe for Consecutively Monitoring Tumor Growth Kinetics and In Situ Screening Inhibitors

Telomerase has long been considered as a biomarker for cancer diagnosis and a therapeutic target for drug discovery. Detecting telomerase activity in vivo could provide more direct information of tumor progression and response to drug treatment, which, however, is hampered by the lack of an effective probe that can generate an output signal without a tissue penetration depth limit. Here in this study, using the principle of distance-dependent magnetic resonance tuning, we constructed a telomerase-activated magnetic resonance imaging probe (TAMP) by connecting superparamagnetic ferroferric oxide nanoparticles (SPFONs) and paramagnetic Gd-DOTA (Gd(III) 1,4,7,10- tetraazacyclododecane-1,4,7,10-tetraazacyclododecane-1,4,7,10-tet-raacetic acid) complexes via telomerase-responsive DNA motifs. Upon telomerase-catalyzed extension of the primer in TAMP, Gd-DOTA-conjugated oligonucleotides can be liberated from the surface of SPFONs through a DNA strand displacement reaction, restoring the T 1 signal of the Gd-DOTA for a direct readout of the telomerase activity. Here we show that, by tracking telomerase activity, this probe provides consistent monitoring of tumor growth kinetics during progression and in response to drug treatment and enables in situ screening of telomerase inhibitors in whole-animal models. This study provides an alternative toolkit for cancer diagnosis, treatment response assessment, and anticancer drug screening.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Probing Electronic and Structural Transformations during Thermal Reduction of the Promising Water Splitting Perovskite BaCe 0.25 Mn 0.75 O 3

Here, in this report, we investigate the thermal reduction of the octahedral perovskite BaCe 0.25 Mn 0.75 O 3 (BCM) using in situ electron energy loss spectroscopy (EELS) in an aberration-corrected transmission electron microscope (TEM). The 12R-polytype of BCM is known to demonstrate high solar thermochemical hydrogen production capacity. In situ EELS measurements show that Mn is the active redox cation in BCM, undergoing thermal reduction from Mn 4+ to Mn 3+ during heating to 700 °C inside the TEM under a high vacuum. The progressive reduction of Mn 4+ during oxygen vacancy (O v ) formation was monitored as a function of temperature. Additionally, atomic-resolution scanning transmission electron microscopy identified two different types of twin boundaries present in the oxidized and reduced form of 12R-BCM, respectively. These two types of twin boundaries were shown, via computational modeling, to modulate the site-specific O v formation energies in 12R-BCM. It is concluded that these types of atomic defects provide sites more energetically favorable for O v formation during thermal reduction.

36 MATERIALS SCIENCE↗

Tribal Revegetation Project Final Project Report: 92-Acre Area, Area 5 Radioactive Waste Management Complex, Nevada National Security Site, Nevada

Nuwu (Southern Paiute), Newe (Western Shoshone), and Nuumu (Owens Valley Paiute) are linguistically related, Numic-speaking peoples who are part of the broader Uto-Aztecan language group. Numic peoples view the land as a holistic, living, sentient being with feelings and purpose. The land is personified with human characteristics and it needs to be experienced to be understood through “learning by doing.” Numic peoples do not support ground disturbing activities within their ancestral lands, including activities tied to the storage of low-level radioactive waste or classified materials on the NNSS, which they view as culturally inappropriate. These deep-rooted ancestral connections are the impetus for reinforcing Numic responsibility for healing disturbed areas by integrating respect and patience with consistent Tribal interaction. Tribal Ecological Knowledge (TEK) is the science of describing Tribal approaches for understanding natural resources. Numic TEK is embedded in traditional teachings learned incrementally over time though experience and it evolves through lessons learned and responses to environmental changes over millennia. Therefore, TEK can broaden and enhance Western scientific knowledge associated with revegetation, especially in highly disturbed areas. The project blended TEK with Western scientific ecological methods to create a vegetative cover within test plots on the 92-Acre Area located at the Radioactive Waste Management Complex (RWMC) located in Area 5 on the Nevada National Security Site (NNSS). The vegetated test plots were systematically created for the Department of Energy (DOE) in tandem with the existing Federal Facilities Agreement and Consent Order (FFACO) with the Nevada Department of Environmental Protection (NDEP). Three previous contractor-lead attempts at revegetation, one targeting full cover revegetation and two targeting test plot revegetation, did not achieve the anticipated results at this location. When presented to the 16 American Indian Tribal nations and affiliated groups with cultural and historical ties to the NNSS, the group appointed a Tribal Revegetation Committee (TRC) that included six expert Tribal knowledge holders to collaborate with an ethnoecologist/cultural anthropologist and two biologists. Project design, planning, seed and outplant selection, spiritual land preparation, and methodology were guided by the TRC and an ethnoecologist/cultural anthropologist from Portland State University (PSU) and biologists from Desert Research Institute (DRI). Using TEK, the TRC recommended a specific seed mixture that contained nine native plant species and three species of outplants. The revegetation effort included preparing and planting thirty 10 m × 10 m (32.8 ft × 32.8 ft) seeded plots, twelve of which also included outplants; and eight 10 m × 100 m (32.8 ft × 328 ft) plots that only received outplants, all atop a waste cell cover. The TRC and the project team creatively adapted TEK with Western scientific methods so that the planned revegetation efforts could occur within the safety and security parameters of the RWMC. Test plots were subjected to one of five soil treatments with varying combinations of straw or mulch applications, soil amendment, and/or outplant planting and one of two watering treatments (watered or unwatered). Planting was divided into two events: one in the fall season and another during the subsequent spring season based on TEK and a corresponding recommendation from the TRC. Monitoring and spiritual management was conducted by the TRC to evaluate plant progress on a monthly basis (in conjunction with the biologist and anthropologist) during each respective growing season for a period of three years after planting. This approach allowed Tribal members the opportunity to conduct traditional blessings and other culturally appropriate activities to restore balance to the land in accordance with Tribal protocols. Following TEK-guided methods, successful plant establishment from seed stock and outplants was observed in many plots. Overall, plots planted in the spring, as recommended by the TRC, showed higher rates of outplant survival and native seedling emergence than those planted in the fall. This finding is significant because it is contrary to the original guidance and previous approaches provided for planting in this region. The TRC believes the frequent co-occurrence of native seedlings near surviving outplants indicates an important symbiotic relationship understood by Tribal communities and overlooked by others. Watered outplants displayed much higher survivability than unwatered plants, even after watering was reduced after the plants were established. Soil amendments and mulch created higher densities of native plants from seed. Many native seedlings showed significant delays in germination, which is considered a normal adaptation to desert climates. Some native plants did not germinate until the third growing year, whereas others germinated during the first growing year, which demonstrates the complexity of the desert environment. Evidence of native insects, reptiles, mammals, and birds, as well as native plants that were not part of the planted species, were noted and considered culturally significant. Despite the presence of non-native plants, native outplants continued to thrive and the incidence of native plant germination from seed increased over time. These successful revegetation results where previous efforts were unsuccessful reinforce the importance of integrating regionally appropriate, TEK-guided methodology with Western science to achieve positive results and the necessity of integrating Tribal involvement in all stages of the revegetation effort. Expanded approaches coupled with Tribal knowledge and tools from Western science addressed a complex problem tied to revegetating atop a low-level radioactive waste cell. The level of Tribal participation serves as a progressive model for building collaborative relationships and addressing ecological challenges on the NNSS.

54 ENVIRONMENTAL SCIENCES↗

Advancements of On-Line Monitoring and Real-Time Characterization of Actual Low-Level Hanford Tank-Waste Samples

Waste and process stream characterization is essential to the safe, efficient, and cost-effective processing of Hanford Site materials. The demand for materials characterization including chemical, radionuclide, and physical attributes during waste management, transfer, and staging operations in the Hanford tank farms is expected to significantly increase as the U.S. Department of Energy River Protection Project progresses toward Hanford waste treatment and immobilization. Our past work has applied Raman on-line monitoring and chemometric modeling to the supernate of Hanford tank 241-AP-105 and has demonstrated the quantitative measurement of nine analytes within this waste. To demonstrate broader applicability, this method has now been expanded to waste from two additional Hanford tanks, AW-102 and AP-107, and the offgas condensate from lab-scale melter runs of actual-tank waste feeds. To enhance the performance of the Raman method, instruments with various excitation wavelengths were compared, since it is known that the Raman response is enhanced by shorter wavelength excitation. The laser excitations for the three systems tested were 404 nm, 532 nm, and 671 nm. In addition to laser wavelength selection, the laser power, measurement integration times, and signal averaging techniques were also investigated to determine their effect on detection limits for oxy-anion analytes within tank wastes. These advancements in Raman capability are compared with past standards and will be presented.

Bryan, Samuel A.↗

International Offshore Geologic Carbon Storage Story Map

The International Offshore Geologic Carbon Storage Story Map provides an overview of offshore geologic carbon storage (GCS) project maturity on a global scale. This story map reviews the history of CO2 storage in offshore sedimentary basins, basic geologic requirements, project timelines and lessons learned through defined project stage progression, areas previously studied for potential future domestic offshore GCS development, and regulatory considerations within different global regions. This resource is a distillation of information collected and recorded in the Offshore Geologic Carbon Storage Inventory Version 1.0 (https://edx.netl.doe.gov/dataset/offshore-gcs-data-inventory).

Assessment↗

Changes in Physical Properties of Everglades Peat Soils Induced by Increased Salinity at the Laboratory Scale: Implications for Changes in Biogenic Gas Dynamics

Abstract Saltwater intrusion due to sea level rise is a major concern for the Florida Everglades because it may induce shifts in ecosystem productivity and physical soil properties. However, the effects of saline water intrusion into the current carbon gas dynamics of the Everglades (particularly in terms of biogenic gas production and emissions, i.e., CH 4 and CO 2 ) are still uncertain. In this work, we present a laboratory‐based study to simulate how sea level rise may alter the physical properties (i.e., hydraulic conductivity) of peat soils from the Everglades and consequently affect the accumulation and release of biogenic gases within the peat matrix. Peat monoliths collected from the Everglades were subjected to progressive increases in salinity from a NaCl solution, and changes to the biogenic gas dynamics regime were simultaneously monitored using a combination of time‐lapse ground‐penetrating radar measurements, manometers, time‐lapse photography, and gas traps. Physical changes to the peat matrix at each salinity interval were assessed using constant head permeameter tests. Consistent with previous research, results show that a progressive increase in salinity (from fresh to saltwater) results in (1) a progressive increase in hydraulic conductivity and (2) a progressive decrease in gas content within the peat matrix (i.e., production) and gas releases. This work has implications for better understanding the potential effects of saltwater intrusion into freshwater peatland systems in the Everglades, particularly in terms of carbon gas dynamics.

Sirianni, Matthew J.↗

Monitoring, Evaluating, and Updating Your Community Solar Action Plan

The Community Solar Action Plan is a living document that guides community solar planning action over time. As conditions change, new information becomes available, or actions progress over the life of the plan, adjustments may be necessary to maintain the plan's relevance. This fact sheet describes some things to consider when implementing, monitoring, evaluating, and updating the Community Solar Action Plan over time.

14 SOLAR ENERGY↗

Current UAS Capabilities for Geospatial Spectral Solutions

Unmanned aerial systems (UAS) are playing an increasingly pivotal role in advancing the remote collection of data. The various datasets collected by these cutting-edge systems provide near-real-time information and help inform end users on everything from disaster recovery efforts to monitoring atmospheric constituents. This chapter focuses on how the collection of imagery has progressed over time, including the pivotal role UAS play in building large-scale datasets driving data analysis. The authors report the findings of a 2018–2021 survey of the fields that use UAS, the UAS platforms most commonly used, and the sensor components users select to outfit their UAS. The chapter will also discuss the range of platforms currently available, with a specific focus on issues including battery life, data downlinks, data processing times, edge computing, and availability of new sensors. The authors will also discuss the future direction of UAS platforms as well as many of the problems associated with the high spatial and temporal resolution of modern geospatial datasets.

Cotten, David↗

Enhanced H 2 production from subsurface through organic acids stimulation Report

Geologic hydrogen (H 2 ) generation through serpentinization of ultramafic rocks presents a promising alternative to conventional hydrogen production, utilizing Earth's abundant magnesium- and iron-rich minerals. This study explores the use of short-chain organic acids (specifically malic acid, MA; and citric acid, CA) to enhance hydrogen yields by stimulating mineral dissolution and iron oxidation from ultramafic rock samples. Batch experiments were conducted with crushed peridotite samples from the Tamarack Complex (Minnesota) and Del Puerto Ophiolite (California). Experiments employed 0.05 M and 0.1 M solutions of MA or CA, under 500 psi (35 bar) nitrogen pressure, at temperatures up to 150 °C. Real-time gas monitoring, aqueous chemistry and solid-phase analyses were performed to track reaction progress and hydrogen production.

08 HYDROGEN↗

Enhanced H 2 production from subsurface through organic acids stimulation

Geologic hydrogen (H₂) generation through serpentinization of ultramafic rocks presents a promising alternative to conventional hydrogen production, utilizing Earth's abundant magnesium- and iron-rich minerals. This study explores the use of short-chain organic acids (specifically malic acid, MA; and citric acid, CA) to enhance hydrogen yields by stimulating mineral dissolution and iron oxidation from ultramafic rock samples. Batch experiments were conducted with crushed peridotite samples from the Tamarack Complex (Minnesota) and Del Puerto Ophiolite (California). Experiments employed 0.05 M and 0.1 M solutions of MA or CA, under 500 psi (35 bar) nitrogen pressure, at temperatures up to 150 °C. Real-time gas monitoring, aqueous chemistry and solid-phase analyses were performed to track reaction progress and hydrogen production.

08 HYDROGEN↗

Engaging the Regulatory Community to Aid Environmental Consenting/Permitting Processes for Marine Renewable Energy

Regulators involved in consenting/permitting marine renewable energy (MRE) have faced multiple challenges due to relatively new, unfamiliar technologies and uncertainty surrounding potential environmental impacts. This has resulted in slow progress for the MRE industry, including long consenting timeframes and extensive and expensive monitoring requirements, which increase financial risk for investors. OES-Environmental has surveyed regulators internationally to understand their key knowledge gaps and perspectives to support the development of the MRE industry. From the results of these surveys a data transferability process and a risk retirement pathway have been developed to assess consenting and monitoring requirements in proportion to risk. A tool for discovering existing data sets by using an online matrix has been developed, along with training materials, regulatory guidance documents, and a strategic outreach plan to engage regulators and advisers. his engagement and the application of these products should lead to a better understanding of the environmental effects of marine energy, and more efficient consenting processes.

16 TIDAL AND WAVE POWER↗

A comprehensive and synthetic dataset for global, regional, and national greenhouse gas emissions by sector 1970–2018 with an extension to 2019

To track progress towards keeping global warming well below 2 °C or even 1.5 °C, as agreed in the Paris Agreement, comprehensive up-to-date and reliable information on anthropogenic emissions and removals of greenhouse gas (GHG) emissions is required. Here we compile a new synthetic dataset on anthropogenic GHG emissions for 1970–2018 with a fast-track extension to 2019. Our dataset is global in coverage and includes CO 2 emissions, CH 4 emissions, N 2 O emissions, as well as those from fluorinated gases (F-gases: HFCs, PFCs, SF 6 , NF 3 ) and provides country and sector details. We build this dataset from the version 6 release of the Emissions Database for Global Atmospheric Research (EDGAR v6) and three bookkeeping models for CO 2 emissions from land use, land-use change, and forestry (LULUCF). We assess the uncertainties of global greenhouse gases at the 90 % confidence interval (5th–95th percentile range) by combining statistical analysis and comparisons of global emissions inventories and top-down atmospheric measurements with an expert judgement informed by the relevant scientific literature. We identify important data gaps for F-gas emissions. The agreement between our bottom-up inventory estimates and top-down atmospheric-based emissions estimates is relatively close for some F-gas species (~ 10 % or less), but estimates can differ by an order of magnitude or more for others. Our aggregated F-gas estimate is about 10 % lower than top-down estimates in recent years. However, emissions from excluded F-gas species such as chlorofluorocarbons (CFCs) or hydrochlorofluorocarbons (HCFCs) are cumulatively larger than the sum of the reported species. Using global warming potential values with a 100-year time horizon from the Sixth Assessment Report by the Intergovernmental Panel on Climate Change (IPCC), global GHG emissions in 2018 amounted to 58 ± 6.1 GtCO 2 eq. consisting of CO 2 from fossil fuel combustion and industry (FFI) 38 ± 3.0 GtCO 2 , CO 2 -LULUCF 5.7 ± 4.0 GtCO 2 , CH 4 10 ± 3.1 GtCO 2 eq., N2O 2.6 ± 1.6 GtCO 2 eq., and F-gases 1.3 ± 0.40 GtCO 2 eq. Initial estimates suggest further growth of 1.3 GtCO 2 eq. in GHG emissions to reach 59 ± 6.6 GtCO 2 eq. by 2019. Our analysis of global trends in anthropogenic GHG emissions over the past 5 decades (1970–2018) highlights a pattern of varied but sustained emissions growth. There is high confidence that global anthropogenic GHG emissions have increased every decade, and emissions growth has been persistent across the different (groups of) gases. There is also high confidence that global anthropogenic GHG emissions levels were higher in 2009–2018 than in any previous decade and that GHG emissions levels grew throughout the most recent decade. While the average annual GHG emissions growth rate slowed between 2009 and 2018 (1.2 % yr –1 ) compared to 2000–2009 (2.4 % yr –1 ), the absolute increase in average annual GHG emissions by decade was never larger than between 2000–2009 and 2009–2018. Our analysis further reveals that there are no global sectors that show sustained reductions in GHG emissions. There are a number of countries that have reduced GHG emissions over the past decade, but these reductions are comparatively modest and outgrown by much larger emissions growth in some developing countries such as China, India, and Indonesia. There is a need to further develop independent, robust, and timely emissions estimates across all gases. As such, tracking progress in climate policy requires substantial investments in independent GHG emissions accounting and monitoring as well as in national and international statistical infrastructures. The data associated with this article (Minx et al., 2021) can be found at https://doi.org/10.5281/zenodo.5566761.

54 ENVIRONMENTAL SCIENCES↗

A New Approach to Monitoring Solvent Extraction Processes for the Nuclear Industry

As part of an initiative to steward research, development, and innovation into the nuclear fuel cycle, Idaho National Laboratory is building the Beartooth testbed. Beartooth will include a cascade of centrifugal contactors, glove box lines, solidification, and dissolution equipment to aid in the progression of novel separation techniques and provide hands-on opportunities to early-career separation scientists. Beartooth will incorporate novel monitoring techniques using sensors and machine learning algorithms to inform a process operator of separation conditions. This research is examining monitoring technologies not typically used in nuclear separation processes such as acoustic microphones, accelerometers, infrared cameras, red-green-blue color sensors, among others. These sensors are being examined for their functionality within a separation process and their ability to detect applicable signals. Machine learning methods are being developed to determine their utility in detecting faults and alerting operators of expected and unexpected events. These methods have the potential to impact Safeguards by Design efforts and real-time decision making. This overview will detail preliminary results from acoustic, vibration, and color sensors.

11 NUCLEAR FUEL CYCLE AND FUEL MATERIALS↗

Screening IMS Seismic Detections using Dynamic Correlation Processor: Progress report for 2020

LLNL completed the FY20 workplan entailing application of the Dynamic Correlation Processor (DCP) to International Monitoring System (IMS) primary seismic data in 2019 and 2020. The effective analysis period was curtailed to January 1 through November 12, 2019 due to a data compression change that rendered later data unusable. Fixing the data compression error is a priority for our data source, and we plan to extend the period of data analysis when the fix is implemented. We found that the percentage overlap between detections generated by the International Data Centre (IDC) DFX software and detections generated by DCP varied widely by station. Overlap is 30-40% at some stations, suggesting that a large percentage of DFX detections could be screened (removed) from the detection-event association algorithm. However, detection overlap was much lower, a few percent, at many stations and the percentage overlap was low at stations known to have many mines and other local sources of seismicity. Unexpectedly low detection overlap at many stations prompted us to examine results for the ARCES station in detail. There is considerable mine activity near ARCES, but our initial FY20 analysis found only ~2% DCP/DFX detection overlap. Differences in the pre-processing (e.g. beam recipes) used by DCP and DFX appear to be the cause of low detection overlap at ARCES. DCP uses wideband filters to improve detector robustness and the IDC uses narrow-band pre-filtering to improve sensitivity. After reprocessing ARCES using the IDC beam recipe, DCP/DFX overlap increased to ~74%. This result shows that we must reprocess the IMS network using the IDC station-specific beam recipes if we are to effectively screen DFX detections and improve automatic event building performance at the IDC.

58 GEOSCIENCES↗

URANIUM HOLDUP MONITORING WITH COMPTON IMAGING AS FUNCTION OF DEPTH AND MASS

Mass quantification using standoff gamma detection is a logical progression for MC&A applications in uranium processing facilities. While obtaining a ground truth from physical collection and weighing of masses is ideal and routine, it poses a logistical problem from both a standpoint of ALARA programs as well as interruptions to operations. The standard approach is the Generalized Geometry in Holdup (GGH) method that can apply for many cases where geometries of the holdup match simulated models; however, there are many cases where a model may not exist or a feature of the holdup geometry is such that it does not match a model particularly well.1 Gamma imaging at standoff distances and outside process equipment has potential to address these shortcomings and improve mass estimations while reducing personnel exposure; however, algorithms still require a significant degree of benchmarking to build sufficient confidence in algorithms before displacing GGH in some procedures. This paper presents the creation of a set of laboratory standards of depleted U3O8 in fixed size containers covering a range of masses and effective thicknesses up to the infinite thickness for the 185-keV line. These blocks are identically sized aluminum boxes loaded such that a thin, flat face is placed towards the imager, while the back side contains closed cell foam to apply pressure to keep the powder in a fixed position at the front face as the sources are manipulated for measurements. Included is an analysis of the mass estimation using a commercially available Compton imaging instrument where multiple geometries are created showing limitations in current methodologies.

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

A General Autofluorescence Method to Characterize Polymerization Progress

Abstract An autofluorescence technique to characterize polymerization progress in real time/in line was developed, which functioned in the absence of typical fluorogenic groups on the monomer or polymer. The monomer dicyclopentadiene and polymer polydicyclopentadiene are hydrocarbons that lack traditional functional groups for fluorescence spectroscopy. Here, the autofluorescence of formulations containing this monomer and polymer during ruthenium‐catalyzed ring‐opening metathesis polymerization (ROMP) was harnessed for reaction monitoring. The methods fluorescence recovery after photobleaching (FRAP) and here‐developed fluorescence lifetime recovery after photobleaching (FLRAP) characterized polymerization progress in these native systems—without requiring exogenous fluorophore. (Auto)fluorescence lifetime recovery changes during polymerization correlated linearly to degree of cure, providing a quantitative link with reaction progress. These changing signals also provided relative rates of background polymerization, enabling comparison of 10 different catalyst‐inhibitor‐stabilized formulations. Multiple‐well analysis demonstrated suitability for future high‐throughput evaluation of formulations for thermosets. The central concept of the combined autofluorescence and FLRAP/FRAP method may be extendable to monitoring other polymerization reactions previously overlooked for lack of an obvious fluorescence handle.

López, Pía A.↗

Offshore Geologic Carbon Storage (GCS) Inventory

The Offshore Geological Carbon Storage Inventory Web Map is an online web mapping application designed to help users explore and visualize the Offshore Geologic Carbon Storage Inventory. This dataset is an inventory of offshore geologic carbon storage (GCS) projects and studies, gathered to summarize ongoing GCS efforts taking place offshore globally. This inventory includes both actualized projects as well as characterization studies and aggregates a variety of attribute fields for each project / study. It is intended to be used for research and comparison purposes, see full disclaimer and credits.

Assessment↗