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DOE OSTI2022

Identification and demonstration of roGFP2 as an environmental sensor for cryogenic correlative light and electron microscopy

Cryogenic correlative light and electron microscopy (cryo-CLEM) seeks to leverage orthogonal information present in two powerful imaging modalities. While recent advances in cryogenic electron microscopy (cryo-EM) allow for the visualization and identification of structures within cells at the nanometer scale, information regarding the cellular environment, such as pH, membrane potential, ionic strength, etc., which influences the observed structures remains absent. Fluorescence microscopy can potentially be used to reveal this information when specific labels, known as fluorescent biosensors, are used, but there has been minimal use of such biosensors in cryo-CLEM to date. Here we demonstrate the applicability of one such biosensor, the fluorescent protein roGFP2, for cryo-CLEM experiments. At room temperature, the ratio of roGFP2 emission brightness when excited at 425 nm or 488 nm is known to report on the local redox potential. When samples containing roGFP2 are rapidly cooled to 77 K in a manner compatible with cryo-EM, the ratio of excitation peaks remains a faithful indicator of the redox potential at the time of freezing. Using purified protein in different oxidizing/reducing environments, we generate a calibration curve which can be used to analyze in situ measurements. As a proof-of-principle demonstration, we investigate the oxidation/reduction state within vitrified Caulobacter crescentus cells. The polar organizing protein Z (PopZ) localizes to the polar regions of C. crescentus where it is known to form a distinct microdomain. Finally, by expressing an inducible roGFP2-PopZ fusion we visualize individual microdomains in the context of their redox environment.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2025

The Effects of Shockwave Pressures on Ultrafast Vibrational Energy Transfer in BNFF, a Hydrogen-Free Energetic Material

Energy conversion in energetic materials from shock-wave-induced lattice compression to bond breaking critically depends on vibrational coupling and energy transfer between intra- and intermolecular vibrations, though the details of the mechanisms remain unknown. Herein, we indirectly tune the strength of intermolecular interactions in 3,4-bis(3-nitrofurazan-4-yl)furoxan (BNFF), a hydrogen-free energetic material characterized by van der Waals interactions, by applying high static pressure using a diamond anvil cell and monitoring vibrational energy transfer (VET) with ultrafast broadband infrared pump–probe spectroscopy. As BNFF is compressed from ambient pressure to 9 GPa, we find that VET accelerates by ∼ 0.9 ps/GPa. Density functional theory is applied in tandem with experiments to assign mode character and elucidate VET pathways. In conclusion, we find that furazan ring O–N–O vibrations, which are high-frequency detonation-relevant vibrational modes, experience increased sensitivity to lattice compression under shockwave pressures. These findings provide new mechanistic insight into how intermolecular interactions govern the rate and selectivity of VET.

Energy transfer↗

DOE OSTI2025

Confinement of Lewis Acid–Base Sites by Microporous Silica Layers on Titania for Enhanced Alkanol Dehydration Reactivity

Alkanol dehydration offers a pathway to upgrade biomass-derived short-chain oxygenates into alkenes, essential chemical building blocks widely used in industrial applications. Transition metal oxides with Lewis acid-base site pairs are attractive catalysts due to their high reactivity and cost-effectiveness. This work demonstrates a synthetic pathway to manipulate local environments around active Lewis acid-base pairs in anatase TiO 2 to enhance their reactivity in alkanol dehydration. Microporous SiO 2 layers with an average pore diameter of ~0.6 nm and a controlled thickness of 0.8-33 nm are deposited on anatase TiO 2 powders by using a molecular templated SiO 2 deposition method. The Lewis acid-base strength of accessible Ti-O pairs remains unchanged, as shown by temperature-programmed surface reactions of surface-bound formic acid-derived species and temperature-programmed desorption of pyridine. However, measured alkanol dehydration rates on confined Ti-O pairs are much higher (by up to 7-fold) than those on TiO 2 . The extent of rate enhancements depends on the reactant size and functional group positioning, suggesting that the rate enhancements reflect the interactions between the guest molecules (reactants and transition states) and the surrounding SiO 2 micropore environments. By providing a detailed synthetic procedure to tailor the local environments around active sites in bulk oxides, this approach offers an additional avenue for enhancing catalytic performance.

09 BIOMASS FUELS↗

NASA NTRS1986

Fatigue response of notched laminates subjected to tension-compression cyclic loads

The fatigue response of a ((0/45/90/-45)(sub s))(sub 4) T300-5208 graphite-epoxy laminate with a drilled center-hole subjected to various components of tensile and compressive cyclic loads was investigated. Damage evaluation techniques such as stiffness monitoring, penetrant-enhanced X-ray radiography, C-scan, laminate deply and residual strength measurement were used to establish the mechanisms of damage development as well as the effect of such damage on the laminate strength, stiffness and life. Damage modes consisted of transverse matrix cracks, initiating at the hole, in all plies, followed by delamination between plies of different orientation. A characteristic stiffness repsonse during cyclic loading at two load levels was identified and utilized a more reliable indicator of material and residual properties than accumulated cycles. For the load ratios of tension-compression loading, residual tensile strength increased significantly above the virgin strength early in the fatigue life and remained approximately constant to near the end of life. A technique developed for predicting delamination initiation sites along the hole boundary correlated well with experimental evidence.

Bakis, C. E.↗

NASA NTRS2022

Satellites quantify the spatial extent of cyanobacterial blooms across the United States at multiple scales

Previous studies indicate that cyanobacterial harmful algal bloom (cyanoHAB) frequency, extent, and magnitude have increased globally over the past few decades. However, little quantitative capability is available to assess these metrics of cyanoHABs across broad geographic scales and at regular intervals. Here, the spatial extent was quantified from a cyanobacteria algorithm applied to two European Space Agency satellite platforms—the MEdium Resolution Imaging Spectrometer (MERIS) onboard Envisat and the Ocean and Land Colour Instrument (OLCI) onboard Sentinel-3. CyanoHAB spatial extent was defined for each geographic area as the percentage of valid satellite pixels that exhibited cyanobacteria above the detection limit of the satellite sensor. This study quantified cyanoHAB spatial extent for over 2,000 large lakes and reservoirs across the contiguous United States (CONUS) during two time periods: 2008–2011 via MERIS and 2017–2020 via OLCI when cloud-, ice-, and snowfree imagery was available. Approximately 56% of resolvable lakes were glaciated, 13% were headwater, isolated, or terminal lakes, and the rest were primarily drainage lakes. Results were summarized at national-, regional-, state-, and lake-scales, where regions were defined as nine climate regions which represent climatically consistent states. As measured by satellite, changes in national cyanoHAB extent did have a strong increase of 6.9% from 2017 to 2020 (|Kendall’s tau (τ)| = 0.56; gamma (γ) = 2.87 years), but had negligible change (|τ| = 0.03) from 2008 to 2011. Two of the nine regions had moderate (0.3 ≤ |τ| < 0.5) increases in spatial extent from 2017 to 2020, and eight of nine regions had negligible (|τ| < 0.2) change from 2008 to 2011. Twelve states had a strong or moderate increase from 2017 to 2020 (|τ| ≥ 0.3), while only one state had a moderate increase and two states had a moderate decrease from 2008 to 2011. A decrease, or no change, in cyanoHAB spatial extent did not indicate a lack of issues related to cyanoHABs. Sensitivity results of randomly omitted daily CONUS scenes confirm that even with reduced data availability during a short four-year temporal assessment, the direction and strength of the changes in spatial extent remained consistent. We present the first set of national maps of lake cyanoHAB spatial extent across CONUS and demonstrate an approach for quantifying past and future changes at multiple spatial scales. Results presented here provide water quality managers information regarding current cyanoHAB spatial extent and quantify rates of change.

Harmful algal blooms↗

NASA NTRS2022

Satellites Quantify the Spatial Extent of Cyanobacterial Blooms Across the United States at Multiple Scales

Previous studies indicate that cyanobacterial harmful algal bloom (cyanoHAB) frequency, extent, and magnitude have increased globally over the past few decades. However, little quantitative capability is available to assess these metrics of cyanoHABs across broad geographic scales and at regular intervals. Here, the spatial extent was quantified from a cyanobacteria algorithm applied to two European Space Agency satellite platforms—the MEdium Resolution Imaging Spectrometer (MERIS) onboard Envisat and the Ocean and Land Colour Instrument (OLCI) onboard Sentinel-3. CyanoHAB spatial extent was defined for each geographic area as the percentage of valid satellite pixels that exhibited cyanobacteria above the detection limit of the satellite sensor. This study quantified cyanoHAB spatial extent for over 2,000 large lakes and reservoirs across the contiguous United States (CONUS) during two time periods: 2008–2011 via MERIS and 2017–2020 via OLCI when cloud-, ice-, and snow-free imagery was available. Approximately 56% of resolvable lakes were glaciated, 13% were headwater, isolated, or terminal lakes, and the rest were primarily drainage lakes. Results were summarized at national-, regional-, state-, and lake-scales, where regions were defined as nine climate regions which represent climatically consistent states. As measured by satellite, changes in national cyanoHAB extent did have a strong increase of 6.9% from 2017 to 2020 (|Kendall’s tau (τ)| = 0.56; gamma (γ) = 2.87 years), but had negligible change (|τ| = 0.03) from 2008 to 2011. Two of the nine regions had moderate (0.3 ≤ |τ| < 0.5) increases in spatial extent from 2017 to 2020, and eight of nine regions had negligible (|τ| < 0.2) change from 2008 to 2011. Twelve states had a strong or moderate increase from 2017 to 2020 (|τ| ≥ 0.3), while only one state had a moderate increase and two states had a moderate decrease from 2008 to 2011. A decrease, or no change, in cyanoHAB spatial extent did not indicate a lack of issues related to cyanoHABs. Sensitivity results of randomly omitted daily CONUS scenes confirm that even with reduced data availability during a short four-year temporal assessment, the direction and strength of the changes in spatial extent remained consistent. We present the first set of national maps of lake cyanoHAB spatial extent across CONUS and demonstrate an approach for quantifying past and future changes at multiple spatial scales. Results presented here provide water quality managers information regarding current cyanoHAB spatial extent and quantify rates of change.

Harmful Algal Blooms↗

DOE OSTI2024

Tensile Deformation of DED Graded IN718-René41 Superalloy at 650°C by in-situ Neutron Diffraction and Digital Image Correlation

A compositionally graded material comprised IN718 (718, γ'/γ" strengthened superalloy) and René41 (R41, a medium γ' strengthened superalloy) was fabricated using laser blown-powder directed energy deposition (DED/LB-M). Hot gas path components that require high and low temperature capabilities at different locations may benefit from such graded superalloy, potentially eliminating the use of welded or mechanically joined components and reducing cost. In the present work, microstructure and 650°C tensile behavior of 718-R41 graded superalloy in as-built and heat-treated states are investigated by Digital Image Correlation (DIC), in-situ neutron diffraction, coupled with conventional characterization techniques. The applied heat treatment generates static recrystallization and equiaxed grains in 718 rich compositions, while R41 rich compositions remain partially or un-recrystallized. Yield strengths of 718 and R41 sections in heat-treated state are comparable to wrought forms but the graded compositions show weakness. From neutron experiment, diffraction elastic constants and single crystal elastic constants are calculated, which show small difference between the as-built and heat-treated states and between the γ and γ' phases. Grain-level load transfer from the (220) grains to (200) grains is found to be composition dependent, and qualitatively agrees with DIC measured macroscopic yield strength. Within the (200) grains, the γ/γ' phases deform elastically until the γ phase yields and afterwards the γ' phase takes load from γ phase.

digital image correlation↗

DOE OSTI2022

Quasiparticle excitations in a one-dimensional interacting topological insulator: Application for dopant-based quantum simulation

Here, we study the effects of electron-electron interactions on the charge excitation spectrum of the spinful Su-Schrieffer-Heeger (SSH) model, a prototype of a one-dimensional bulk obstructed topological insulator. In view of recent progress in the fabrication of dopant-based quantum simulators we focus on experimentally detectable signatures of interacting topology in finite lattices. To this end we use Lanczos-based exact diagonalization to calculate the single-particle spectral function in real space which generalizes the local density of states to interacting systems. Its spatial and spectral resolution allows for the direct investigation and identification of edge states. By studying the noninteracting limit, we demonstrate that the topological in-gap states on the boundary are robust against both finite-size effects as well as random bond and onsite disorder which suggests the feasibility of simulating the SSH model in engineered dopant arrays in silicon. While edge excitations become zero-energy spinlike for any finite interaction strength, our analysis of the spectral function shows that the single-particle charge excitations are gapped out on the boundary. Despite the loss of topological protection, we find that these edge excitations are quasiparticlelike as long as they remain within the bulk gap. Above a critical interaction strength of U c ≈5t these quasiparticles on the boundary lose their coherence which is explained by the merging of edge and bulk states. This is in contrast to the many-body edge excitations which survive the limit of strong coupling, as established in the literature. Our findings show that for moderate repulsive interactions the nontrivial phase of the interacting SSH model can be detected through remnant signatures of topological single-particle states using single-particle local measurement techniques such as scanning tunneling spectroscopy.

75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND↗

NASA NTRS2016

Formation of Oxides in the Interior of Friction Stir Welds

In friction stir welding (FSWing) the actual solid state joining takes place between the faying surfaces which form the weld seam. Thus the seam trace is often investigated for clues when the strength of the weld is reduced. Aluminum and its alloys are known to form a native, protective oxide on the surface. If these native surface oxides are not sufficiently broken up during the FSW process, they are reported to remain in the FSW interior and weaken the bond strength. This type of weld defect has been referred to as a lazy "S", lazy "Z", joint line defect, kissing bond, or residual oxide defect. Usually these defects are mitigated by modification of the process parameters, such as increased tool rotation rate, which causes a finer breakup of the native oxide particles. This study proposes that there may be an alternative mechanism for formation of oxides found within the weld nugget. As the oxidation rate increases at elevated temperatures above 400ºC, it may be possible for enhanced oxidation to occur on the interior surfaces during the FSW process from entrained air entering the seam gap. Normally, FSWs of aluminum alloys are made without a purge gas and it is unknown how process parameters and initial fit up could affect a potential air path into the interior during the processing. In addition, variations in FSW parameters, such as the tool rotation, are known to have a strong influence on the FSW temperature which may affect the oxidation rate if internal surfaces are exposed to entrained air. A series of FSWs were made in 3 different thickness panels of AA2219 (0.95, 1.27 and 1.56 cm) at 2 different weld pitches. As the thickness of the panels increased, there was an increased tendency for a gap to form in advance of the weld tool. If sufficient air is able to enter the workpiece gap prior to consolidation, the weld temperature can increase the oxidation rate on the interior surfaces. These oxidation rates would also be accelerated in areas of localized liquation. Metallographs from the weld panels showed indications of liquation at the grain boundaries. In FSWs of thicker panels, these regions of liquation were found to be heavily oxidized. The quality of the FSWs was evaluated from tensile testing at room temperature. As the panel thickness increased, a slight decrease in tensile strength was observed which was attributed to the presence of oxides. No oxide formation was observed in the thinner workpieces, although there were indications of localized liquation at the grain boundaries. Results from this study will assist in a better understand of the mechanisms of oxide formation in FSW interiors and provide methodology for minimizing their occurrence.

Schneider, Judy↗

DOE OSTI2022

Ultrastrong Coupling of Band‐Nested Excitons in Few‐Layer Molybdenum Disulphide

Abstract The 2D transition‐metal dichalcogenides (2D TMDCs) are an intriguing platform for studying strong light–matter interactions because they combine the electronic properties of conventional semiconductors with the optical resonances found in organic systems. However, the coupling strengths demonstrated in strong exciton–polariton coupling in the 2D TMDCs remain much lower than those found in organic systems. In this paper, a new approach is taken by utilizing the large oscillator strength of the above‐band gap C exciton in few‐layer molybdenum disulphide (FL‐MoS 2 ). A k ‐space Rabi splitting of 293 meV is shown when coupling FL‐MoS 2 C excitons to surface plasmon polaritons at room temperature. This value is 11% of the uncoupled exciton energy (2.67 eV or 464 nm), ≈2× what is typically seen in the TMDCs, placing the system in the ultrastrong coupling regime. The results take a step toward finally achieving the efficient quantum coherent processes of ultrastrong coupling in a CMOS‐compatible system—the 2D TMDCs—in the visible spectrum.

36 MATERIALS SCIENCE↗

DOE OSTI2020

β Decay of 61 V and its Role in Cooling Accreted Neutron Star Crusts

The interpretation of observations of cooling neutron star crusts in quasipersistent x-ray transients is affected by predictions of the strength of neutrino cooling via crust Urca processes. The strength of crust Urca neutrino cooling depends sensitively on the electron-capture and β -decay ground-state-to-ground-state transition strengths of neutron-rich rare isotopes. Nuclei with a mass number of A = 61 are predicted to be among the most abundant in accreted crusts, and the last remaining experimentally undetermined ground-state-to-ground-state transition strength was the β decay of 61 V. This Letter reports the first experimental determination of this transition strength, a ground-state branching of $8.1^{+4.0}_{- 3.1}%$, corresponding to a log ft value of $5.5^{+0.2}_{-0.2}$. This result was achieved through the measurement of the β -delayed γ rays using the total absorption spectrometer SuN and the measurement of the β -delayed neutron branch using the neutron long counter system NERO at the National Superconducting Cyclotron Laboratory at Michigan State University. This method helps to mitigate the impact of the pandemonium effect in extremely neutron-rich nuclei on experimental results. The result implies that A = 61 nuclei do not provide the strongest cooling in accreted neutron star crusts as expected by some predictions, but that their cooling is still larger compared to most other mass numbers. Finally, only nuclei with mass numbers 31, 33, and 55 are predicted to be cooling more strongly. However, the theoretical predictions for the transition strengths of these nuclei are not consistently accurate enough to draw conclusions on crust cooling. With the experimental approach developed in this work, all relevant transitions are within reach to be studied in the future.

79 ASTRONOMY AND ASTROPHYSICS↗

DOE OSTI2023

Strengthening mechanisms, hardening/softening behavior, and microstructure evolution in an LPSO magnesium alloy at elevated temperatures

Here, rapid degradation of the mechanical properties of conventional Mg alloys with temperature precludes their wide application in industry. The LPSO (long period stacking ordered) Mg alloys, on the other hand, show considerable potential to achieve excellent high-temperature mechanical performance. In this study, a Mg-5.1Y-2.4Zn (wt.%) extrusion alloy with the microstructure comprising of α-Mg matrix and LPSO phase was prepared. It is shown that the yield strength of the alloy at 200 °C retained ~83% of the yield strength of 198 MPa at room temperature (RT). Importantly, the ductility also improved, owing to the increased non-basal slip activities at elevated temperatures. In contrast to RT and 200 °C, the alloy exhibited softening tensile behavior with an apparent loss of yield strength at 300 °C. In-situ lattice strain analysis revealed that load transfer from α-Mg to LPSO remains effective at temperatures up to 200 °C, governing the high yield strength of the alloy. The post-mortem analysis by means of electron microscopy confirmed that the LPSO phase is structurally stable at 200 °C. In particular, the high strain hardenability is rationalized by the sandwiched LPSO structures together with stacking faults serving as effective obstacles to the motion of non-basal dislocations. The load transfer effect disappears at the temperature of 300 °C, resulting in the fast deterioration of strength of the alloy. The cracking of the LPSO phase along with the recrystallizing of α-Mg grains were found to be the main causes for the softening behavior of the alloy at 300 °C.

36 MATERIALS SCIENCE↗

NASA NTRS1993

Interaction strength and model geometry effects on the structure of crossing-shock wave/turbulent boundary-layer interactions

The flowfield structure of a range of symmetric crossing-shock wave/turbulent boundary-layer interactions of varying strength is presented. The test geometry, consisting of a symmetric pair of opposing sharp fins at angle of attack, alpha, mounted to a flat plate, is studied experimentally for a range of alpha from 7 to 15 degrees at Mach numbers of 3 and 4. Results reveal that the basic flowfield shock structure remains similar in nature over the range of interaction strengths examined, with the only changes being in the scale and location of the various features present. The separated flow regions are classified as being either completely or partially separated, the completely separated case being the one in which the entire incoming boundary layer separates from the plate surface. For the current experiments, all but the weakest of the interactions exhibited complete boundary layer separation. Finally, the effects of model geometry are analyzed by comparing data for shock generators of varying lengths, with the results showing no evidence of upstream influence due to the shock generator trailing edges.

Garrison, T. J.↗

NASA NTRS1943

Bearing Strengths of Some Wrought-aluminum Alloys

Although a number of investigations of the bearing strength of aluminum alloys have been made, the problem remains one of considerable interest to the aircraft industry. For this reason it has seemed advisable to make additional tests of the commonly used aircraft alloys in an effort to establish a better basis for the selection of allowable bearing values. Current design practice does not recognize the effect of edge distance upon bearing strengths, and for this reason edge distance was one of the principal variables considered in this investigation. The increasing emphasis being placed upon permanent set limitations makes it essential that more information on bearing yield phenomena be obtained. The object of this investigation was to determine bearing yield and ultimate strengths of the following aluminum alloy products: 17S-T, 24S-T, Alclad 24S-T, 24S-RT, 52S-0, 52S-1/2H, 52S-H, 53S-T, and 61S-T extrusions. Ratios of these bearing properties to tensile properties were also determined.

Moore, R L↗

DOE OSTI2025

Quality Investigation of Pultruded Carbon Fiber Panels Subjected to Four-Point Flexure via Fiber Optic Sensing

Pultruded carbon fiber-reinforced composites are attractive to the wind energy industry due to the rapid production of highly aligned unidirectional composites with enhanced fiber volume fractions and increased specific strength and stiffness. However, high volume carbon fiber manufacturing remains cost-prohibitive. This study investigates the feasibility of a pultruded low-cost textile carbon fiber-reinforced epoxy composite as a promising material in spar cap production was undertaken based on mechanical response to four-point flexure loading. As spar caps are primarily subjected to flexural loading, large-span four-point flexure was considered, and coupon testing was restricted to tensile modulus and compression strength assessment. High-resolution spatial fiber optic strain sensing was utilized to determine spatial strain distribution during four-point flexure, revealing consistent strain along the length of the part and proved to be an excellent option for process manufacturing quality examination. Additionally, holes with diameters of 2.49 mm, 5.08 mm, and 1.93 mm were drilled through the thickness of full-width parts to determine the feasibility of structural health monitoring of pultruding parts internal to wind blades via fiber optic strain sensing.

Chemistry↗

DOE OSTI2024

High‐Pressure Synthesis and Recovery of Single Crystals of the Metastable Manganese Carbide, MnC x

Abstract Transition metal carbides find widespread use throughout industry due to their high strength and resilience under extreme conditions. However, they remain largely limited to compounds formed from the early d‐block elements, since the mid‐to‐late transition metals do not form thermodynamically stable carbides. We report here the high‐pressure bulk synthesis of large single crystals of a novel metastable manganese carbide compound, MnC x (P6 3 /mmc), which adopts the anti‐NiAs‐type structure with significant substoichiometry at the carbon sites. We demonstrate how synthesis pressure modulates the carbon loading, with ~40 % occupancy being achieved at 9.9 GPa.

Chemistry↗

DOE OSTI2022

The effect of Ti content on age hardening and mechanical properties of uranium-titanium alloys

Here, the effect of Ti content on age hardening and the resulting mechanical properties are described for -quenched U-Ti alloys containing 0.3 wt.% to 2.0 wt.%Ti. Age hardening occurs between ~250°C and ~450°C. Overaging occurs at higher temperatures by cellular decomposition. Age hardening kinetics suggest that different mechanisms occur depending on Ti content and initial microstructure. Strengthening in 'a acicular martensites begins by the formation of Ti clusters which evolve into thin U 2 Ti disc shaped precipitates and later mature into continuous U 2 Ti rods beginning at peak hardness. The mechanism of hardening in 'b banded martensite is more elusive, as significant hardening occurs where atomic mobility is lower than that required for precipitate formation, similar to that reported for age hardening in ''b banded martensite in U-6%Nb. The activation energy for aging varies with Ti content and microstructure. In fully martensitic alloys containing 0.75% to 2.0%Ti it is in the vicinity of ~44 kcal/mole (184 kJ/mole). But it is lower in alloys containing less than 0.6%Ti where quenched microstructures are less than fully martensitic. Tensile ductility is high prior to aging, decreases with age hardening, is effectively zero at peak hardness, and remains low in overaged conditions. Attractive combinations of strength and ductility are best obtained in alloys containing 0.6% to 1.0%Ti which have been partially aged to fractional hardening levels no greater than ~0.6. This corresponds to the very early stages of aging, associated with clustering and the earliest stages of U 2 Ti disc formation. Alloys containing 0.45%Ti or less are not as responsive to age hardening. Alloys containing 1.5% and 2.0%Ti can be aged to higher strengths, but extreme quench rate sensitivity prevents them from being effectively heat treated in realistic section thicknesses.

36 MATERIALS SCIENCE↗

DOE OSTI2022

16s Amplicon Analysis of Soil Data for Interactive effects of depth and differential irrigation on soil microbiome composition and functioning

Genomic DNA was isolated from soil and rhizosphere samples using the Zymo Quick-DNA fecal/soil microbe miniprep kit (catalog no. D6010) according to the manufacturer’s instructions (Zymo Research; Irvine, CA) with the modification of eluting in 100 uL elution buffer. Sample concentrations were quantified using the Qubit dsDNA HS assay kit (Thermo Fisher). For rhizosphere samples only, DNA was subsequently purified using Zymo’s ZR-96 DNA Clean & Concentrator kit (catalog no. D4024) to account for low DNA concentrations of these samples. . In each replicate block, there were five drip irrigation treatments (T1 = 100% normal irrigation, T2 = 56.25%, T3 = 37.5%, T4 = 18.75% and T5=no irrigation. On July 20, the strength of the drought treatments was increased: T1 remained at 100%, whereas T2 changed from 75% to 56.25%, T2 changed from 50% to 37.5%, and T4 changed from 25% to 18.75%. Sequencing was performed as described previously (Naylor, Fansler, et al. 2020). Sequences were amplified on the MiSeq platform (Illumina, San Diego, CA) using 16S primers (515F and 806R) specific to the V4 region. Raw sequence data was processed with the pipeline Hundo for amplicon quality control and annotation. Downstream statistical analyses on 16S datasets were performed using the program R and the packages ‘phyloseq’ and ‘vegan’.

Soil microbiome, metatranscriptomics↗