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At least 127 records · Page 7

In vitro functional analysis of gRNA sites regulating assembly of hepatitis B virus

The roles of RNA sequence/structure motifs, Packaging Signals (PSs), for regulating assembly of an HBV genome transcript have been investigated in an efficient in vitro assay containing only core protein (Cp) and RNA. Variants of three conserved PSs, within the genome of a strain not used previously, preventing correct presentation of a Cp-recognition loop motif are differentially deleterious for assembly of nucleocapsid-like particles (NCPs). Cryo-electron microscopy reconstruction of the T = 4 NCPs formed with the wild-type gRNA transcript, reveal that the interior of the Cp shell is in contact with lower resolution density, potentially encompassing the arginine-rich protein domains and gRNA. Symmetry relaxation followed by asymmetric reconstruction reveal that such contacts are made at every symmetry axis. We infer from their regulation of assembly that some of these contacts would involve gRNA PSs, and confirmed this by X-ray RNA footprinting. Mutation of the ε stem-loop in the gRNA, where polymerase binds in vivo, produces a poor RNA assembly substrate with Cp alone, largely due to alterations in its conformation. The results show that RNA PSs regulate assembly of HBV genomic transcripts in vitro, and therefore may play similar roles in vivo, in concert with other molecular factors.

99 GENERAL AND MISCELLANEOUS↗

A probabilistic fatigue analysis of multiple site damage

The variability in initial crack size and fatigue crack growth is incorporated in a probabilistic model that is used to predict the fatigue lives for unstiffened aluminum alloy panels containing multiple site damage (MSD). The uncertainty of the damage in the MSD panel is represented by a distribution of fatigue crack lengths that are analytically derived from equivalent initial flaw sizes. The variability in fatigue crack growth rate is characterized by stochastic descriptions of crack growth parameters for a modified Paris crack growth law. A Monte-Carlo simulation explicitly describes the MSD panel by randomly selecting values from the stochastic variables and then grows the MSD cracks with a deterministic fatigue model until the panel fails. Different simulations investigate the influences of the fatigue variability on the distributions of remaining fatigue lives. Six cases that consider fixed and variable conditions of initial crack size and fatigue crack growth rate are examined. The crack size distribution exhibited a dominant effect on the remaining fatigue life distribution, and the variable crack growth rate exhibited a lesser effect on the distribution. In addition, the probabilistic model predicted that only a small percentage of the life remains after a lead crack develops in the MSD panel.

Rohrbaugh, S. M.↗

Integrating Radar, Multispectral, and Landing Site Data for Analysis of the Lunar Surface

Radar maps of the Moon have been produced since the late 1960s, and have been used by a number of authors to study the surface roughness, subsurface rock abundance, and dielectric properties of the lunar surface. These studies focused on a range of topics, including the depth and rock population of the regolith, crater ejecta blankets, pyroclastic mantling layers, and cryptomare deposits. Limited radar sounding data from the Apollo missions identified layering in some regions of the maria. As radar datasets have improved in resolution and calibration, it has become more possible to make quantitative comparisons between the backscatter properties of the Moon, other remote-sensing observations, and the ground truth provided by Surveyor photos and Apollo traverses. This presentation will focus on the results of recent studies of the lunar regolith that make use of these diverse sources of information, and discuss research directions that will be possible with radar data to be collected in the near future.

Campbell, B. A.↗

Mars Delay-Doppler Radar Observations With GSSR: Global Analysis for Landing Site Selection and Characterization

Earth-based radar data remain an important part of the information set used to select and certify spacecraft landing sites on Mars. Constraints on robotic landings on Mars include: terrain elevation, radar reflectivity. regional and local slopes, rock distribution and coverage, and surface roughness, all of which are addressed by radar data. Indeed, the usefulness of radar data for Mars exploration has been demonstrated in the past. Radar data were critical in assessing the Viking Lander I site, and more recently, the Mars Pathfinder landing site.

Haldemann, A. F. C.↗

Global Database of GSSR Mars Delay-Doppler Radar Obsevations: Analysis for Landing Site Characterization and Rover Trafficability

Earth-based radar data remain an important part of the information set used to select and certify spacecraft landing sites on Mars. Constraints on robotic landings on Mars include: terrain elevation, radar reflectivity, regional and local slopes, rock distribution and coverage, and surface roughness, all of which are addressed by radar data. Indeed, the usefulness of radar data for Mars exploration has been demonstrated in the past. Radar data were critical in assessing the Viking Lander, and more recently, the Mars Pathfinder landing.

Haldemann, A. F. C.↗

Global Database of GSSR Mars Delay-Doppler Radar Observations: Analysis for Landing Site Characterization and Rover Trafficability

Earth-based radar data remain an important part of the information set used to select and certify spacecraft landing sites on Mars. Constraints on robotic landings on Mars include: terrain elevation, radar reflectivity, regional and local slopes, rock distribution and coverage, and surface roughness, all of which are addressed by radar data. Indeed, the usefulness of radar data for Mars exploration has been demonstrated in the past. Radar data were critical in assessing the Viking Lander 1 site, and more recently, the Mars Pathfinder landing site.

Haldemann, A. F. C.↗

Upcoming Lunar Mission Landing Sites: Viewing and Analysis with Celestial Mapping System

The Celestial Mapping System (CMS) is a powerful software platform designed to create virtual 3D globes for celestial bodies within our solar system, with a specific focus on lunar mapping. CMS offers a rich array of functionalities, including high-resolution imagery visualization, precise measurement tools, and analytical capabilities that cater to the needs of planetary scientists and mission planners. This abstract explores how CMS has been leveraged to conduct in-depth analyses of 12 lunar missions to the South Pole, including missions like Chandraayan 3, Russian Space Agency's Luna 25, Xelene 1, and Intuitive Machines Nova-C lander I. Moreover, the integration of HORUS (Hyper-effective nOise Removal Unit Software) into CMS enhances its capabilities, allowing for the rendering of images in a 3D globe format and providing visibility into permanently shadowed regions. CMS represents a cutting-edge tool for lunar surface awareness, equipment placement planning, and traverse path optimization

Moon↗

Annual Summary Report (FY 2025) Composite Analysis for Low-Level Waste Disposal in the Central Plateau of the Hanford Site

In accordance with DOE M 435.1-1, Radioactive Waste Management Manual, requirements, the U.S. Department of Energy (DOE) Hanford Field Office (HFO) prepared this Annual Summary Report (ASR) for fiscal year (FY) 2025 for the Hanford Site Composite Analysis (CA) reported in DOE/RL-2019-52, Composite Analysis for Low-Level Waste Disposal in the Hanford Site Central Plateau (FY 2020) (hereinafter referred to as the Hanford Site Composite Analysis). The preparation of this ASR follows the technical specifications provided in DOE-STD-5002-2017, Disposal Authorization Statement and Tank Closure Documentation.

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

Composite Analysis for Low-Level Waste Disposal in the Hanford Site Central Plateau (FY 2020) (Rev. 2)

This document provides an updated Hanford Site composite analysis (CA). A Hanford Site CA was prepared and issued in 1998 (PNNL-11800) with an addendum provided in 2002 (PNNL-11800 Addendum 1). The CA was approved in 2002 (Frei, 2002) and has been maintained (DOE/RL-2000-29 and subsequent revisions) to support low-level waste disposal performance assessments (PAs) and disposal authorizations for facilities at the Hanford Site, including the following: Continued operation of the Environmental Restoration Disposal Facility (ERDF) and the 200 East and 200 West Low-Level Burial Grounds; Construction of the Integrated Disposal Facility (IDF); Forthcoming closure of tank residual waste systems such as Waste Management Area (WMA) C. The CA maintenance program resulted in a determination in 2015 (DOE/RL-2015-66) that the Hanford Site CA needed an update for the following reasons: While the initial Hanford Site CA has been maintained since 2001, the accumulation of basis changes reported in the annual summary reports over the succeeding 14 years merit evaluation in an updated analysis; The U.S. Department of Energy (DOE) Headquarters requested in a memorandum in 2015 (Gilbertson and Marcinowski, 2015) that “as soon as the relevant PAs are complete, the CA will be revised to account for all of the new information.” This updated Hanford Site CA accounts for the following new information: 1. Inclusion of a detailed Hanford Site baseline disposition that projects remedial activities through site closure. There have been significant changes through decision making in the Comprehensive Environmental Response, Compensation, and Liability Act of 1980 (CERCLA) process that were not available when the original CA was produced; 2. Inclusion of an updated inventory basis, new modeling capabilities, and new decisions reached in the associated record of decision (ROD) that was provided by issuance of DOE/EIS-0391 in fiscal year (FY) 2013. Development of a Hanford Site groundwater model from the baseline provided in a technical transfer of models for the Final Tank Closure and Waste Management Environmental Impact Statement (DOE/EIS-0391) commenced in FY 2014 and resulted in the plateau-to-river (P2R) groundwater flow model that is used in this updated Hanford Site CA; 3. Accounting for pump and treat systems, which were not evaluated in the initial CA. Pump and treat systems have had significant impact on groundwater flow system behavior, contaminant transport, and contaminant removal from Hanford Site groundwater. This process is accounted for in the use of the P2R model for this updated Hanford Site CA; 4. Inclusion of water-level data collected since the initial CA was completed. Data collected as the unconfined aquifer water levels continue to recede since the cessation of large liquid discharges in the late 1990s have led to marked improvement in understanding of the flow system for future conditions, particularly regarding northward flow potential in the critical Gable Gap area. The calibration in the latest version of the P2R model accounts for this information; 5. Inclusion of results from the revised ERDF PA completed in 2013 (WCH-5209) that account for updated inventory and expansion of the ERDF facility to about twice the size that was evaluated in the original CA; 6. Use of updated geoframeworks to provide the structural basis for numerical fate and transport models in the groundwater pathway of this updated CA. The geologic basis for groundwater models has continued to improve with additional data collection and interpretation with the creation and maintenance of the Hanford South Geoframework and the Central Plateau Vadose Zone Geoframework tools; 7. Incorporation of updated tank residual inventory estimates. Tank residual inventory estimates have improved with the incorporation of tank retrieval inventory data for those tanks that have completed retrieval. The CA inventory data package includes this updated information; 8. Incorporation of WMA C PA results. Two additional PAs for tank farm closure decisions are in preparation during the period required to prepare an updated Hanford Site CA: WMA C (FY 2016) and WMA A-AX (in preparation). The WMA C PA results are incorporated into the updated Hanford Site CA, and its grouted residuals model is used as the basis for a release model to account for the other tank farm systems modeling in this updated CA; 9. An update to the IDF PA was submitted in FY 2017 and has been reviewed and approved. The results of the IDF PA are incorporated into this updated CA; 10. Updated risk assessment scenarios. The risk assessment scenarios currently in use for Hanford Site CERCLA and Resource Conservation and Recovery Act of 1976 (RCRA) analyses differ from those evaluated in the initial Hanford Site CA. The representative person exposure scenario evaluated in this updated Hanford Site CA is consistent with recent PAs and CERCLA and RCRA analyses as the Hanford Site. This updated Hanford Site CA provides the following: A comparison of the updated Hanford Site CA all-pathway dose results with the performance measures during the compliance period, which is assumed to begin with site closure in calendar 2070 with the last scheduled disposal action and continue for 1,000 years postclosure (to calendar year 3069); A comparison of Hanford Site CA all-pathway dose results with the performance measures during the postcompliance period to address potential peaks beyond the compliance period. This is accomplished by evaluating dose in the period for 9,000 years following the compliance period (i.e., from calendar year 3070 to 12070).

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Composite Analysis for Low Level Waste Disposal in the Central Plateau of the Hanford Site (FY2019 Annual Status Report)

In accordance with DOE M 435.1-1 requirements and as implemented by DOE/RL-2000-29, the U.S. Department of Energy (DOE), Richland Operations Office has prepared this annual summary for fiscal year (FY) 2019. Originally reported in PNNL-11800 and PNNL-11800, Addendum 1, the Hanford Site Composite Analysis was approved through issuance of a 2002 memorandum. As required by DOE/RL-2000-29, an annual evaluation of new information and data developed by a number of onsite programs was completed. The reporting period for this annual evaluation is FY 2019 (October 1, 2018, through September 30, 2019). The information provided in this evaluation includes activities performed in FY 2019 that are considered pertinent to the Hanford Site Composite Analysis - information that could change source terms considered, and monitoring, research, and development results.

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Solid Waste Release Calculations for the Composite Analysis Baseline Assessment

The purpose of the Hanford Site Composite Analysis (CA) is to provide an estimate of the cumulative radiological impacts from active and planned low-level radioactive waste disposals and other potentially interacting radioactive waste disposal sources that will remain following Hanford Site closure. The need for a waste form release model addressing multiple solid waste forms is documented in CP-60410, Hanford Site Composite Analysis Technical Approach Description: Waste Form Release.

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Data-Driven Surface Traversability Analysis for Mars 2020 Landing Site Selection

The objective of this paper is three-fold: 1) to describe the engineering challenges in the surface mobility of the Mars 2020 Rover mission that are considered in the landing site selection processs, 2) to introduce new automated traversability analysis capabilities, and 3) to present the preliminary analysis results for top candidate landing sites. The analysis capabilities presented in this paper include automated terrain classification, automated rock detection, digital elevation model (DEM) generation, and multi-ROI (region of interest) route planning. These analysis capabilities enable to fully utilize the vast volume of high-resolution orbiter imagery, quantitatively evaluate surface mobility requirements for each candidate site, and reject subjectivity in the comparison between sites in terms of engineering considerations. The analysis results supported the discussion in the Second Landing Site Workshop held in August 2015, which resulted in selecting eight candidate sites that will be considered in the third workshop.

Ono, Masahiro↗

Analysis of surface moisture variations within large field sites

A statistical analysis was made on ground soils to define the general relationship and ranges of values of the field moisture relative to both the variance and coefficient of variation for a given test site and depth increment. The results of the variability study show that: (1) moisture variations within any given large field area are inherent and can either be controlled nor reduced; (2) neither a single value of the standard deviation nor coefficient of variation uniquely define the variability over the complete range of mean field moisture contents examined; and (3) using an upper bound standard deviation parameter clearly defines the maximum range of anticipated moisture variability. 87 percent of all large field moisture content standard deviations were less than 3 percent while about 96 percent of all the computed values had an upper bound of sigma=4 percent for these intensively sampled fields. The limit of accuracy curves of mean soil moisture measurements for large field sites relative to the required number of samples were determined.

Bell, K. R.↗

Two Mars years of surface changes seen at the Viking Landing sites

An analysis of the spectrophotometric properties of the new dust deposited at the Viking Lander 1 site indicates that the new deposits are the same as the bright red material that only partially covered the site at the beginning of the mission. Certain areas of the Viking 1 site also showed an increase in contrast during the second year. The contrast increase is thought to be due to the partial removal of the recently deposited dust cover by high-velocity winds. What is more, a small soil slump occurred near the base of a boulder lying about 25 m from the lander. It is noted that whereas no obvious erosion has been detected at the Viking 2 site, periodic removal of the dust cover at this site is implied by the abundance of rocks at the site.

Guinness, E. A.↗