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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 127 records · Page 7

The Kimberlina synthetic multiphysics dataset for CO 2 monitoring investigations

Abstract We present a synthetic multi‐scale, multi‐physics dataset constructed from the Kimberlina 1.2 CO 2 reservoir model based on a potential CO 2 storage site in the Southern San Joaquin Basin of California. Among 300 models, one selected reservoir‐simulation scenario produces hydrologic‐state models at the onset and after 20 years of CO 2 injection. Subsequently, these models were transformed into geophysical properties, including P‐ and S‐wave seismic velocities, saturated density where the saturating fluid can be a combination of brine and supercritical CO 2 , and electrical resistivity using established empirical petrophysical relationships. From these 3D distributions of geophysical properties, we have generated synthetic time‐lapse seismic, gravity and electromagnetic responses with acquisition geometries that mimic realistic monitoring surveys and are achievable in actual field situations. We have also created a series of synthetic well logs of CO 2 saturation, acoustic velocity, density and induction resistivity in the injection well and three monitoring wells. These were constructed by combining the low‐frequency trend of the geophysical models with the high‐frequency variations of actual well logs collected at the potential storage site. In addition, to better calibrate our datasets, measurements of permeability and pore connectivity have been made on cores of Vedder Sandstone, which forms the primary reservoir unit. These measurements provide the range of scales in the otherwise synthetic dataset to be as close to a real‐world situation as possible. This dataset consisting of the reservoir models, geophysical models, simulated time‐lapse geophysical responses and well logs forms a multi‐scale, multi‐physics testbed for designing and testing geophysical CO 2 monitoring systems as well as for imaging and characterization algorithms. The suite of numerical models and data have been made publicly available for downloading on the National Energy Technology Laboratory's (NETL) Energy Data Exchange (EDX) website.

58 GEOSCIENCES↗

Charged Wellbore Casing Controlled Source Electromagnetics (CWC-CSEM) for Reservoir Imaging and Monitoring (Final report)

This project addresses the needs of the U.S. Department of Energy (DOE) to develop advanced monitoring technologies and protocols to track the fate of subsurface carbon dioxide (CO2) plumes for carbon storage. Specifically, the project seeks to develop and test a unique and novel system of technologies consisting of electromagnetic data acquisition, coupled multiphysics imaging, and reservoir model enhancement to understand the migration and long-term distribution of CO2 in the subsurface. The overarching objective is to develop an integrated approach for long term monitoring of carbon storage. The two main components of the project include the methodology development and the test of the method at a field site. The methodology component consists of 1) developing the field procedure and protocol for collecting time-lapse controlled-source electromagnetic (CSEM) data with source electric current injected into the subsurface through wellbore casings; 2) building of background 3D electrical conductivity utilizing multiple sources of data such as supplemental surface transient EM (TEM) surveys, well-logs, and seismic structural information, for enhancing CSEM signal from reservoir depths; and 3) coupled multiphysics simulations and inversion of CSEM data constrained by production data and by structural information from seismic imaging of the reservoir and overlying formations. The testing component used the field site of Bell Creek Oil Field, which served both as a field laboratory for the method development as well as a test site to evaluate the CSEM signal strengths and the methodology developed in this research project. We have accomplished all the proposed tasks and developed the methodology as planned. These include the procedure for time-lapse CSEM data acquisition, data processing techniques, integration with 3D conductivity model building, fast reservoir simulation for history matching using machine learning, and interpreting CSEM data with coupling to the reservoir modeling. Collectively, the outcome of these tasks form a coherent workflow that can be applied to monitor dedicated carbon storage in saline reservoirs. The testing component evaluated the applicability and limitations of the method, and concluded that the method would be ideal for monitoring dedicated carbon storage sites utilizing saline reservoirs.

54 ENVIRONMENTAL SCIENCES↗

Physiological Maturation of Regenerating Hair Cells

The bullfrog saccule, a sensor of gravity and substrate-borne vibration, is a model system for hair cell transduction. Saccular hair cells also increase in number throughout adult life and rapidly recover after hair cell damage, making this organ an ideal system for studying hair cell development, repair, and regeneration. We have used of hair cell and supporting cell immunocytochemical markers to identify damaged hair cells and hair cell precursors in organotypic cultures of the bullfrog saccule. We then used an innovative combination of confocal, electron, and time-lapse microscopy to study the fate of damaged hair cells and the origin of new hair cells after gentamicin ototoxicity in normal and mitotically blocked saccular cultures. These studies have shown that gentamicin ototoxicity produces both lethal and sublethal hair cell damage. They have also shown that hair cell recovery in this organ takes place by both the repair of sublethally damaged hair cells and by the replacement of lost hair cells by mitotic regeneration. In parallel studies, we have used biophysical and molecular biological techniques to study the differentiation and innervation of developing, repairing, and regenerating hair cells. More specifically, we have used RT-PCR to obtain the bullfrog homologues of L-type voltage- gated calcium (L-VGCC) and large-conductance Ca(2+)-activated potassium (BK) channel genes. We have then obtained probes for these genes and, using in situ hybridization, begun to examine their expression in the bullfrog saccule and amphibian papilla. We have also used fluorescent-labeled channel toxins and channel toxin derivatives to determine the time of appearance of L-type voltage-gated calcium (L-VGCC) and Ca(2+)-activated potassium (BK) channels and to study dynamic changes in the number, distribution, and co-localization of these proteins in developing, repairing, and regenerating hair cells. Using time-lapse microscopy, we are also studying the dynamic relationship between ion channel clustering and synaptic formation in hair cells and afferent neurons. In future studies, we will determine when hair cell precursors acquire electrical tuning, and, using whole-cell patch-clamp techniques, identify and characterize their L-VGCC and BK currents. We will also use biophysical techniques to determine the number of L-VGCC and BK channels and the size and gating kinetics of their underlying L-VGCC and BK conductances, correlating these variables with the amplitude and frequency of membrane oscillations produced by intracellular current steps. We expect these studies to determine how hair cells regulate ion channel expression to achieve specific physiological responses.

Baird, Richard A.↗

DoZen Documentation

DoZen (pronounced “do zen”) is for processing, visualizing, and exploring electromagnetic data that is stored in Zonge’s .z3d format. It was created specifically for processing time-lapse controlled source electromagnetic data.

97 MATHEMATICS AND COMPUTING↗

Linear response theory for annealing of radiation damage in semiconductor devices

A theoretical study of the radiation/annealing response of MOS ICs is described. Although many experiments have been performed in this field, no comprehensive theory dealing with radiation/annealing response has been proposed. Many attempts have been made to apply linear response theory, but no theoretical foundation has been presented. The linear response theory outlined here is capable of describing a broad area of radiation/annealing response phenomena in MOS ICs, in particular, both simultaneous irradiation and annealing, as well as short- and long-term annealing, including the case when annealing is nearing completion. For the first time, a simple procedure is devised to determine the response function from experimental radiation/annealing data. In addition, this procedure enables us to study the effect of variable temperature and dose rate, effects which are of interest in spaceflight. In the past, the shift in threshold potential due to radiation/annealing has usually been assumed to depend on one variable: the time lapse between an impulse dose and the time of observation. While such a suggestion of uniformity in time is certainly true for a broad range of radiation annealing phenomena, it may not hold for some ranges of the variables of interest (temperature, dose rate, etc.). A response function is projected which is dependent on two variables: the time of observation and the time of the impulse dose. This dependence on two variables allows us to extend the theory to the treatment of a variable dose rate. Finally, the linear theory is generalized to the case in which the response is nonlinear with impulse dose, but is proportional to some impulse function of dose. A method to determine both the impulse and response functions is presented.

Litovchenko, Vitaly↗

Assessment of Borehole Gravity (Density) Monitoring for CO 2 Injection into the Dover 33 Reef

The Midwest Regional Carbon Sequestration Partnership (MRCSP) was founded in 2003 as part of the U.S. Department of Energy’s (DOE’s) Regional Carbon Sequestration Partnership initiative. Since its founding, MRCSP has made significant strides toward making CCUS a viable option for states in the region. The public/private consortium, funded through the DOE Regional Carbon Sequestration Initiative, brings together nearly 40 industry partners and 10 states. Battelle, as the project lead, oversees research, development and operations and coordinates activities among the partners. The incremental, phased approach has built a valuable knowledge base for the industry and paved the way for commercial-scale adoption of CCUS technologies. From 2008 to 2020, MRCSP Phase III focused on the development of large-scale injection projects. This report is part of a series of reports prepared under the Midwestern Regional Carbon Sequestration Partnership (MRCSP) Phase III (Development Phase). These reports summarize and detail the findings of the work conducted under the Phase III project. This report presents the results of BHG surveys conducted in the Lawnichak-Myskier 1-33 well in the Dover 33 reef by Tellus Gravity and Micro-g LaCoste in 2013, 2016, and 2018. A comparison of the data from the three surveys was performed to determine the feasibility of BHG to detect and monitor the location of the injected CO 2 in the reef over time. In addition, modeling was performed to compare the field data with the modeled data. Applying time-lapse BHG monitoring to a carbon sequestration site consists of determining temporal gravity anomalies related to the injection of CO 2 , and exclusively associated to the redistribution of the fluids in the pore space.

01 COAL, LIGNITE, AND PEAT↗

Soil oxidoreductase zymography: Visualizing spatial distributions of peroxidase and phenol oxidase activities at the root-soil interface

Decomposition of organic material in the rhizosphere – the most dynamic microbial habitat in soil – involves arrays of oxidoreductase and hydrolytic enzymes. Spatial distributions of various hydrolytic activities in soil have already been explored by zymographic techniques. However, the distribution of oxidative activity in the rhizosphere remains to be studied. Thus, we extended a Time-Lapse Zymography technique, using Amplex Red® reagent, to visualize and quantify distributions of phenol oxidase and peroxidase activities in the rhizosphere of Zea mays L. growing in a Haplic Phaeozem and the non-rhizospheric soil. The gross oxidative activity was greatest at the root surfaces, and fell to background soil levels 1.26 and 0.73 mm from seminal (>1 mm diameter) and lateral (<0.5 mm diameter) roots, respectively. The rhizosphere extent relative to the root radius was 59% broader around lateral than around seminal roots. The greatest activities, up to 30 nmol cm –2 min –1 , were peroxidase-dominated and closely associated with roots. Here, the results confirm the utility of the approach for studying spatio-temporal distributions of oxidative activities in soil. However, actual activity of oxidoreductases in the field will be strongly controlled by fluctuating environmental conditions such as soil aeration and the gradient of reactive oxygen species, which need to be considered especially in anoxic soils.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

An automated system for continuous monitoring of CO 2 geosequestration using multi-well offset VSP with permanent seismic sources and receivers: Stage 3 of the CO2CRC Otway Project

Time-lapse seismic is an essential tool for monitoring CO 2 injection into the subsurface but suffers from high cost and long intervals between surveys. An alternative approach is continuous monitoring using permanent sources and receivers capable of mapping CO 2 plume evolution on an almost daily basis and drastically reduce human effort, cost and survey turnaround. As part of the CO2CRC Otway project, a continuous monitoring system is employed to monitor a small (15,000 tonnes) CO 2 injection within the CO2CRC Otway Project. The permanent monitoring system employs distributed acoustic sensors (DAS) installed in boreholes as seismic receivers and seismic orbital vibrators (SOV) as seismic sources. The monitoring approach is based on vertical seismic profiling using five wells equipped with DAS cemented behind the casing and nine SOVs and is acquiring the data continuously in an autonomous manner. The data is then automatically processed on-site producing a seismic image every 2.5 days, which is then transmitted to a remote office. The data has good repeatability with NRMS of about ~10-15%.

58 GEOSCIENCES↗

Seismic monitoring of CO 2 geosequestration using multi-well 4D DAS VSP: Stage 3 of the CO2CRC Otway project

An important part of any CO 2 geosequestration project is to ensure CO 2 containment and conformance in the subsurface. This is generally done by implementing a comprehensive, risk-based Measurement, Monitoring and Verification plan, a key element of which is active time-lapse seismic monitoring. However, high cost and environmental impact of the standard surface seismic monitoring dictate the need for a cost-effective and environmentally friendly alternative. An opportunity to develop such method emerges with advances in distributed acoustic sensing (DAS) technology, which turns an optical fibre into a seismic sensor with dense spatial sampling. DAS can be permanently deployed in multiple wells across the geosequestration site providing a robust and non-intrusive network of seismic receivers. This approach was developed and tested in the CO2CRC Otway project, where injection of 15 kt of CO 2 at 1.5 km depth was monitored with a 4D vertical seismic profiling (VSP) using five borehole DAS arrays and mobile vibroseis sources. The 4D DAS VSP in each of the five wells provides broadly consistent images of the CO 2 plume with some differences due to different illumination of the target horizon, lateral variation of velocities, and seismic anisotropy. When the newly injected CO 2 reaches a CO 2 plume created as a result of an earlier injection into the same formation ~600 m updip, 4D DAS VSP shows a change in reflectivity in that area and beyond. Furthermore, this shows a potential of 4D DAS VSP for monitoring gas injection into gas-saturated reservoirs.

58 GEOSCIENCES↗

Plant-microbial interplay for organic nitrogen mediated by functional specificity of root compartments

The organic form of nitrogen (N) is a critical intermediate in mutualistic and competitive root-microbial interactions, mediated by extracellular enzymes. Visualization of the hotspots of organic N and proteolytic activity might be valuable for revealing root functional specificity in N acquisition and transformation at the level of individual roots and compartments. For the first time, we used time-lapse amino-mapping and zymography to co-localize and map the spatial distribution of amino-N and leucine aminopeptidase (LAP) activity in the soil and different root parts of maize (Zea mays L.). Amino-N distribution was mainly associated with seminal roots and root tips, where it overlapped with LAP activity hotspots. In the lateral roots and bulk soil, however, LAP activity was decoupled from amino-N. Distinct functional traits revealed themselves as the highest amino-N content and LAP activity in seminal root tips and as the largest relative extent of the rhizosphere in lateral root tips. Co-localized amino-N and LAP activities highlighted different nutrient acquisition strategies mediated by root-microbe interactions, depending on the root compartment. Seminal roots and their tips appeared to adopt mutualistic strategies, potentially attracting root-associated microorganisms through releasing oligo- and polypeptides. In contrast, lateral roots, with amino-N detected only at their tips, demonstrated stronger N competition, relying on the enzyme activity of the rhizosphere microbial community for N acquisition. These insights emphasized the role of root functional specialization in shaping plant-microbe interactions, offering pathways to enhance nutrient use efficiency.

Maize (Zea mays L.)↗

AmeriFlux FLUXNET-1F US-Prr Poker Flat Research Range Black Spruce Forest

This is the AmeriFlux Management Project (AMP) created FLUXNET-1F version of the carbon flux data for the site US-Prr Poker Flat Research Range Black Spruce Forest. This is the FLUXNET version of the carbon flux data for the site US-Prr Poker Flat Research Range Black Spruce Forest produced by applying the standard ONEFlux (1F) software. Site Description - This site is located in a blackspruce forest within the property of the Poker Flat Research Range, University of Alaska, Fairbanks. Time-lapse image of the canopy is measured at the same time to relate flux data to satellite images.

Iwahana, Go [University of Alaska, Faribanks]↗

Bacteria Counter

Science Applications, Inc.'s ATP Photometer makes a rapid and accurate count of the bacteria in a body fluid sample. Instrument provides information on the presence and quantity of bacteria by measuring the amount of light emitted by the reaction between two substances. Substances are ATP adenosine triphosphate and luciferase. The reactants are applied to a human body sample and the ATP Photometer observes the intensity of the light emitted displaying its findings in a numerical output. Total time lapse is usually less than 10 minutes, which represents a significant time savings in comparison of other techniques. Other applications are measuring organisms in fresh and ocean waters, determining bacterial contamination of foodstuffs, biological process control in the beverage industry, and in assay of activated sewage sludge.

Source record↗

Building a Bilingual Google Earth Engine Dashboard to Increase Accessibility to Long-term Time Series Remote Sensing Data for Monitoring Saline System Changes in Chile’s Atacama Desert

Saline systems, consisting of salt flats, ponds, and marshes, provide vital water resources to wildlife and communities in northern Chile’s Atacama Desert, one of the driest regions in the world. Mining is extensive in the Atacama, which contains 30% of the world’s lithium reserves and is abundant in potassium and boron. The groundwater that feeds into salt marshes and ponds is extracted in large volumes for mining operations, limiting the availability of water for ecosystems. However, identifying long-term and large-scale environmental impacts from local lithium mining on the saline systems is limited by region inaccessibility and terrain variability. Open access satellite imagery and cloud computing technology has made studying Atacama saline systems feasible and allowed for collaboration across different agencies and countries. The NASA DEVELOP Program partnered with Chile’s la Universidad de La Serena and Servicio Nacional de Geología y Minería (SERNAGEOMIN) to create the Saline Analysis Tool (SalT) in Google Earth Engine (GEE). SalT is used to analyze the extent and distribution of remote saline systems in the Atacama from 1986 to the present day. The tool filters Landsat 5 Thematic Mapper (TM) and Landsat 8 Operational Land Imager (OLI) data from GEE’s data catalog and creates a single composite image per year for analysis. Additional output analyses include land cover classification, Normalized Difference Vegetation Index (NDVI) and Normalized Difference Water Index (NDWI) raster images that can be displayed on the map interface or exported. The tool can also generate time-lapse videos and charts displaying NDVI, NDWI, and land cover over time. A key feature of the tool is the use of a bilingual graphical user interface to make analysis accessible and customizable to different users’ needs—SalT provides options to select an analysis area, analysis time period, and outputs to display or export. The tool also incorporates new Earth observations as they are added to GEE’s catalog. The ability to easily visualize and analyze long-term remote sensing imagery will enable SERNAGEOMIN and la Universidad de la Serena to continually monitor changes in these saline systems and inform future land management policy.

NASA DEVELOP↗

Lab-On-Chip Clinorotation System for Live-Cell Microscopy Under Simulated Microgravity

Cells in microgravity are subject to mechanical unloading and changes to the surrounding chemical environment. How these factors jointly influence cellular function is not well understood. We can investigate their role using ground-based analogues to spaceflight, where mechanical unloading is simulated through the time-averaged nullification of gravity. The prevailing method for cellular microgravity simulation is to use fluid-filled containers called clinostats. However, conventional clinostats are not designed for temporally tracking cell response, nor are they able to establish dynamic fluid environments. To address these needs, we developed a Clinorotation Time-lapse Microscopy (CTM) system that accommodates lab-on- chip cell culture devices for visualizing time-dependent alterations to cellular behavior. For the purpose of demonstrating CTM, we present preliminary results showing time-dependent differences in cell area between human mesenchymal stem cells (hMSCs) under modeled microgravity and normal gravity.

Yew, Alvin G.↗

Lab-On-Chip Clinorotation System for Live-Cell Microscopy Under Simulated Microgravity

Cells in microgravity are subject to mechanical unloading and changes to the surrounding chemical environment. How these factors jointly influence cellular function is not well understood. We can investigate their role using ground-based analogues to spaceflight, where mechanical unloading is simulated through the time-averaged nullification of gravity. The prevailing method for cellular microgravity simulation is to use fluid-filled containers called clinostats. However, conventional clinostats are not designed for temporally tracking cell response, nor are they able to establish dynamic fluid environments. To address these needs, we developed a Clinorotation Time-lapse Microscopy (CTM) system that accommodates lab-on- chip cell culture devices for visualizing time-dependent alterations to cellular behavior. For the purpose of demonstrating CTM, we present preliminary results showing time-dependent differences in cell area between human mesenchymal stem cells (hMSCs) under modeled microgravity and normal gravity.

Yew, Alvin G.↗

Geometrical-Based Generative Adversarial Network to Enhance Digital Rock Image Quality

X-ray microcomputed tomography (micro-CT) is a common tool for the study of porous media structures and properties. High-quality micro-CT data are required to accurately capture pore structures. Acquiring high-quality micro-CT data, however, is not always possible, owing to application limitations and experimental constraints. Therefore, we propose a geometrical-based generative adversarial network (GAN) to rapidly restore noisy micro-CT images to their clean counterparts. The training data and related ground-truth (GT) data are scanned for 7 min and 9.5 h, respectively. To evaluate the performance of the geometrical-based GAN, a 6003 voxel image that has never been used for training is reconstructed and compared with the corresponding GT image. Histogram matching and linear normalization are implemented to adjust the histogram of the reconstructed image to that of the GT image. A watershed-based segmentation method is then applied to delineate pore and solid phases. Lastly, we measure the Minkowski functionals and petrophysical properties, including absolute permeability, pore size distribution, drainage capillary pressure-saturation curve, and imbibition relative permeability, to estimate the physical accuracy of the denoised image. The results show that the proposed geometrical-based GAN can accurately restore noisy micro-CT data. By reducing the scanning time from 9.5 h to 7 min, the expenditure of collecting micro-CT can be decreased significantly. This is particularly important for applications where time-lapse images of a dynamic process are required, high-throughput imaging is necessary for real-time data analysis, or where the quantification of large sample volumes is required.

58 GEOSCIENCES↗

Studies of Model Immiscible Systems

The objectives are to use model transparent monotectics to obtain fundamental information applicable to two-phase systems in general, to apply this understanding to materials of interest in the Microgravity Science and Applications program, and to interpret results of flight experimental involving monotectic alloys. A number of model immiscible systems are in use to study various aspects of two-phase behavior within the miscibility gap and during solidification. Particle growth, coalescence and particle motions are under investigation using a holographic microscopy system. The system is capable of working with particle densities up to 10 to the 7th power particles/cubic centimeters through a 100 micron depth and can resolve particles of the order of 2 to 3 micron in diameter throughout the entire cell volume. Particle size, distribution changes with respect to time and temperature are observable from sequential holograms. Initial experiments using diethylene glycol/ethyl salicylate (DEG/ES) have demonstrated the usefulness of the technique. The thermal system controls temperature to at least plus or minus 0.001 K over the course of an experiment. A time-lapse film, made from holograms, of a succinonitrile/water solution shows particle size and number distribution changes with time under isothermal conditions. The observations are consistent with Ostwald ripening theory.

Frazier, D. O.↗

ESIAS Pseudocode

The Explosive Shock Image Analysis System (ESAIS) is designed to provide a semiautomated quantitative analysis of fast-frame imagery taken during the detonation of high explosive (HE) rate sticks. ESIAS does this by assisting the user in identifying the HE stick geometry on the image (i.e., sides of the undetonated HE stick, detonation front, an axial reference point to assist in the inter-comparison of images); scaling the images based on the known diameter of the undetonated HE stick; identifying the profiles of various surfaces resulting from the detonation process such as the shock wave and product gases or the outer pipe surface; calculating the detonation speed of the HE by tracking the distance it travels between two images with a known time lapse between the images; and determining the slit velocities resulting from the detonation. ESIAS will also import scope traces from oscilloscopes and assist in identifying the time of arrival of significant peaks.

97 MATHEMATICS AND COMPUTING↗