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At least 1,261 records · Page 70

Genomic dissection of anthracnose resistance response in sorghum [ Sorghum bicolor (L.) Moench]

Sorghum [Sorghum bicolor (L.) Moench] is the fifth most important grain crop behind maize, wheat, rice, and barley. Today, it is of interest as a source of fermentable sugars for the production of renewable fuels and chemicals, and as a source of biomass for co-firing. The productivity and profitability of sorghum are limited by several biotic constraints, most notably anthracnose caused by the fungal pathogen Colletotrichum sublineolum. The most cost-effective and environmentally benign strategy to control anthracnose is through the incorporation of resistance genes. Over the last three years, our research efforts have been directed to identify new sources of resistance in temperate adapted and tropical germplasm, and to delimited genomic regions associated with the observe anthracnose resistant response. Three biparental mapping populations derived from the resistant lines SC112-14, QL3 and IS18760 were evaluated for anthracnose resistance response in Texas, Georgia, Florida and Puerto Rico. In parallel, three high density recombination maps were constructed and used to identify resistant loci. Anthracnose resistant response in line SC112-14 is controlled by a major locus on chromosome 5. Segregation analysis of 1,500 progenies delimited the resistance locus on chromosome 5 to a 23-kb region harboring three candidate genes, including Sobic.005G17230 identified by GWAS of the sorghum association panel (SAP). The latter gene belongs to a family of genes encoding F-box proteins indicating that this resistance response involved in signaling cascades and transcriptional reprograming, rather than recognition of pathotype-associated molecular patterns. In contrast, anthracnose resistant response in lines QL3 and IS18760 is controlled by multiple small-effect genes. Greenhouse evaluation of a representative subset of the three mapping populations against nine pathotypes found that lines susceptible in the field could be resistant to a single pathotype in the greenhouse. Thus, the activation of a resistance response system by a single pathotype could not provide a broader resistance response against multiple pathotypes. The screening of 1,801 sweet sorghum accessions from the National Plant Germplasm System identified 654 accessions with Brix value larger than 10, which in turn was used to select a subset of 233 accessions for evaluation of anthracnose resistant response. Even though most of the accessions were not completely infected by anthracnose, 28 accessions were completely resistant against pathotypes from Texas, Georgia, Florida and Puerto Rico. Genotyping-by-sequencing analysis of this subset identified 157,843 single nucleotide polymorphisms. Population structure analysis of the subset based on a subset of 2,345 unlinked SNPs found that the genetic diversity could be divided into four populations. The genetic relatedness among accessions within populations suggests most of the resistant germplasm may contain few different resistance sources. These resistance sources present in sweet sorghum germplasm could expedite the development of new resistant sweet sorghum cultivars and hybrids by avoiding time-consuming introgression breeding approaches with non-sweet sorghums serving as donor of the resistance alleles.

09 BIOMASS FUELS↗

Summary of an integrated ERTS-1 project and its results at the Missouri Geological Survey

Use of the ERTS imagery involved the recognition and interpretation of various ground patterns. Analysis and application are tied to ongoing programs. Specific studies utilizing the imagery and NASA aircraft photography are: a statewide lake and dam inventory; assessment of flooding and floodprone areas along the Missouri portion of the Mississippi and Missouri Rivers; land-use classification for several counties; structural features in selected areas; and Pleistocene features in northern Missouri. Though it has been suggested that repetitive coverage is not necessary for geologic studies, it is this specific feature along with the synoptic view of large portions of the State that provided the potential for the utilization of the ERTS imagery in Missouri. Other State agencies, Departments of Conservation, Agriculture, and Community Affairs, have expressed interest in the potential application of ERTS data in their respective fields.

Martin, J. A.↗

A multilevel approach to sequential detection of pictorial features

The problem of detecting the local similarity between templates in a given class and a given image using a hierarchically ordered sequential decision rule is examined. It is proposed that the set of templates be partitioned and a 'representative template' be defined for each of the partitions. Several levels of partitioning are defined. Elimination of mismatching locations and termination of computation can take place at each level of detection. Each level of testing is over a more restrictive subset of the template class than the previous level. Criteria are given for selecting representative templates, the ordering of components of a template vector for error evaluation, and the threshold sequences to be used in deciding about a 'match'. Suboptimal solutions are given satisfying these criteria. Examples showing recognition of linear features in test patterns and photographs obtained by aerial and spaceborne sensors are provided.

Ramapriyan, H. K.↗

Tectonic evolution of Eastern Ishtar Terra, Venus

Tectonic deformation is cited as the cause for the complex morphology and topography of Eastern Ishtar Terra. A number of models proposed to account for this deformation are reviewed, and their predictions for the geology of Fortuna Tessera are examined. The detailed geology, topography, and structural fabric of Eastern Ishtar are reviewed, and these observations are compared to the predictions of the existing models for the formation and evolution of Fortuna Tessera. On the basis of the analysis, a number of conclusions are drawn regarding the evolution of Eastern Ishtar Terra. These include the recognition of primary morphological and topographical patterns which allow Eastern Ishtar Terra to be divided into four major physiographic regions named Maxwell Montes, Eastern Fortuna Tessera, Central Fortuna Tessera, and Northern Fortuna Tessera. A model is suggested that accounts for the morphology and high topography of Eastern Ishtar Terra.

Vorder Bruegge, R. W.↗

Detecting Faults In Helicopter Gearboxes By The MVIM Method

Multivalued influence-matrix (MVIM) method potential utility as theoretical basis of proposed automated monitoring systems detecting faults in helicopter gearboxes. Applied to recognize patterns in vibration measurements. Fault-recognition system required to operate continuously while helicopter airborne, analyzing measurements of vibrations for signs of trouble to provide real-time warning of any dangerous or potentially dangerous fault like cracked case or fractured gear tooth. System also required not to give false alarms to prevent unnecessary emergency landings.

Chin, Hsinyung↗

Analysis of Land-Use Effects on Landscape Patterns and Biological Diversity in Pacific North Forests: 1972-1991

While there is widespread recognition of the importance of preserving biological diversity there is considerable uncertainty about how to map current patterns of diversity and monitor changes through time. Ground-based approaches are impractical for examining regional patterns of biological diversity, for monitoring change, and they may actually overlook important higher-order phenomena. Thus, there is a critical need for innovative techniques to examine land-use effects on biological diversity at the landscape and regional scales. In this project, we have used satellite-based remote sensing to examine land-use effects on forest ecosystems in the Pacific NorthWest region (PNW) of the U.S.A. Rates and patterns of forest change throughout the region were quantified for the period from 1972 to 1993. This information was then used to map changes in the abundance and distribution of potential habitat for selected vertebrate species. The results of this project will be useful for identifying "keystone" stands that are important in maintaining habitat connectivity at the regional scale and for evaluating the impact of future land-use on vertebrate diversity throughout the region. The approaches developed here will also be useful in other forested regions throughout the world.

Wallin, David O.↗

Automated Classification of Disease Patterns from Echo-cardiography Images Based on Shape Features of the Left Ventricle

Computer assisted diagnosis using analysis of medical images is an area of active research in health informatics. This paper proposes a technique for indication of heart diseases by using information related to shapes of the left ventricle (LV). LV boundaries are tracked from echo-cardiography images taken from LV short axis view, corresponding to two disease conditions viz. dilated cardiomyopathy and hypertrophic cardiomyopathy, and discriminated from the normal condition. The LV shapes are modeled using shape histograms generated by plotting the frequency of normalized radii lengths drawn from the centroid to the periphery, against a specific number of bins. A 3-layer neural network activated by a log-sigmoid function is used to classify the shape histograms into one of the three classes. Experimentations on a dataset of 240 images show recognition accuracies of the order of 80%.

62 RADIOLOGY AND NUCLEAR MEDICINE↗

Pattern-Triggered Immunity Suppresses Programmed Cell Death Triggered by Fumonisin B1

Programmed cell death (PCD) is a crucial process for plant innate immunity and development. In plant innate immunity, PCD is believed to prevent the spread of pathogens from the infection site. Although proper control of PCD is important for plant fitness, we have limited understanding of the molecular mechanisms regulating plant PCD. Plant innate immunity triggered by recognition of effectors (effector-triggered immunity, ETI) is often associated with PCD. However pattern-triggered immunity (PTI), which is triggered by recognition of elicitors called microbe-associated molecular patterns (MAMPs), is not. Therefore we hypothesized that PTI might suppress PCD. Here we report that PCD triggered by the mycotoxin fumonisin B1 (FB1) can be suppressed by PTI in Arabidopsis. FB1-triggered cell death was suppressed by treatment with the MAMPs flg22 (a part of bacterial flagellin) or elf18 (a part of the bacterial elongation factor EF-Tu) but not chitin (a component of fungal cell walls). Although plant hormone signaling is associated with PCD and PTI, both FB1-triggered cell death and suppression of cell death by flg22 treatment were still observed in mutants deficient in jasmonic acid (JA), ethylene (ET) and salicylic acid (SA) signaling. The MAP kinases MPK3 and MPK6 are transiently activated and inactivated within one hour during PTI. We found that FB1 activated MPK3 and MPK6 about 36–48 hours after treatment. Interestingly, this late activation was attenuated by flg22 treatment. These results suggest that PTI suppression of FB1-triggered cell death may involve suppression of MPK3/MPK6 signaling but does not require JA/ET/SA signaling

59 BASIC BIOLOGICAL SCIENCES↗

The chemical structure of DNA sequence signals for RNA transcription

The proposed recognition sites for RNA transcription for E. coli NRA polymerase, bacteriophage T7 RNA polymerase, and eukaryotic RNA polymerase Pol II are evaluated in the light of the requirements for efficient recognition. It is shown that although there is good experimental evidence that specific nucleic acid sequence patterns are involved in transcriptional regulation in bacteria and bacterial viruses, among the sequences now available, only in the case of the promoters recognized by bacteriophage T7 polymerase does it seem likely that the pattern is sufficient. It is concluded that the eukaryotic pattern that is investigated is not restrictive enough to serve as a recognition site.

George, D. G.↗

Correlating transcription and protein expression profiles of immune biomarkers following lipopolysaccharide exposure in lung epithelial cells

Universal and early recognition of pathogens occurs through recognition of evolutionarily conserved pathogen associated molecular patterns (PAMPs) by innate immune receptors and the consequent secretion of cytokines and chemokines. The intrinsic complexity of innate immune signaling and associated signal transduction challenges our ability to obtain physiologically relevant, reproducible and accurate data from experimental systems. One of the reasons for the discrepancy in observed data is the choice of measurement strategy. Immune signaling is regulated by the interplay between pathogen-derived molecules with host cells resulting in cellular expression changes. However, these cellular processes are often studied by the independent assessment of either the transcriptome or the proteome. Correlation between transcription and protein analysis is lacking in a variety of studies. In order to methodically evaluate the correlation between transcription and protein expression profiles associated with innate immune signaling, we measured cytokine and chemokine levels following exposure of human cells to the PAMP lipopolysaccharide (LPS) from the Gram-negative pathogen Pseudomonas aeruginosa . Expression of 84 messenger RNA (mRNA) transcripts and 69 proteins, including 35 overlapping targets, were measured in human lung epithelial cells. We evaluated 50 biological replicates to determine reproducibility of outcomes. Following pairwise normalization, 16 mRNA transcripts and 6 proteins were significantly upregulated following LPS exposure, while only five (CCL2, CSF3, CXCL5, CXCL8/IL8, and IL6) were upregulated in both transcriptomic and proteomic analysis. This lack of correlation between transcription and protein expression data may contribute to the discrepancy in the immune profiles reported in various studies. The use of multiomic assessments to achieve a systems-level understanding of immune signaling processes can result in the identification of host biomarker profiles for a variety of infectious diseases and facilitate countermeasure design and development.

59 BASIC BIOLOGICAL SCIENCES↗

Alinement tool for X-ray image intensifiers

Gage consists of upper plate and lower plate connected by aluminum post marked with metric scale. Upper plate is identical to calibration plate except for post flange. Lower plate is made of aluminum and is grooved for x-y coordinates and pattern of concentric rings that aid in recognition and measurement of nonlinearity. X-y coordinates on upper and lower plates match exactly. Gage indicates amount and direction of skew in images.

Rasmussen, D.↗

A study of image quality for radar image processing

Methods developed for image quality metrics are reviewed with focus on basic interpretation or recognition elements including: tone or color; shape; pattern; size; shadow; texture; site; association or context; and resolution. Seven metrics are believed to show promise as a way of characterizing the quality of an image: (1) the dynamic range of intensities in the displayed image; (2) the system signal-to-noise ratio; (3) the system spatial bandwidth or bandpass; (4) the system resolution or acutance; (5) the normalized-mean-square-error as a measure of geometric fidelity; (6) the perceptual mean square error; and (7) the radar threshold quality factor. Selective levels of degradation are being applied to simulated synthetic radar images to test the validity of these metrics.

King, R. W.↗

Human Expertise Helps Computer Classify Images

Two-domain method of computational classification of images requires less computation than other methods for computational recognition, matching, or classification of images or patterns. Does not require explicit computational matching of features, and incorporates human expertise without requiring translation of mental processes of classification into language comprehensible to computer. Conceived to "train" computer to analyze photomicrographs of microscope-slide specimens of leucocytes from human peripheral blood to distinguish between specimens from healthy and specimens from traumatized patients.

Rorvig, Mark E.↗

Automatic speech recognition research at NASA-Ames Research Center

A trainable acoustic pattern recognizer manufactured by Scope Electronics is presented. The voice command system VCS encodes speech by sampling 16 bandpass filters with center frequencies in the range from 200 to 5000 Hz. Variations in speaking rate are compensated for by a compression algorithm that subdivides each utterance into eight subintervals in such a way that the amount of spectral change within each subinterval is the same. The recorded filter values within each subinterval are then reduced to a 15-bit representation, giving a 120-bit encoding for each utterance. The VCS incorporates a simple recognition algorithm that utilizes five training samples of each word in a vocabulary of up to 24 words. The recognition rate of approximately 85 percent correct for untrained speakers and 94 percent correct for trained speakers was not considered adequate for flight systems use. Therefore, the built-in recognition algorithm was disabled, and the VCS was modified to transmit 120-bit encodings to an external computer for recognition.

Coler, Clayton R.↗

Structural basis of RNA recognition and activation by innate immune receptor RIG-I

Retinoic-acid-inducible gene-I (RIG-I; also known as DDX58) is a cytoplasmic pathogen recognition receptor that recognizes pathogen-associated molecular pattern (PAMP) motifs to differentiate between viral and cellular RNAs. RIG-I is activated by blunt-ended double-stranded (ds)RNA with or without a 5'-triphosphate (ppp), by single-stranded RNA marked by a 5'-ppp and by polyuridine sequences. Upon binding to such PAMP motifs, RIG-I initiates a signalling cascade that induces innate immune defences and inflammatory cytokines to establish an antiviral state. The RIG-I pathway is highly regulated and aberrant signalling leads to apoptosis, altered cell differentiation, inflammation, autoimmune diseases and cancer. The helicase and repressor domains (RD) of RIG-I recognize dsRNA and 5'-ppp RNA to activate the two amino-terminal caspase recruitment domains (CARDs) for signalling. Here, to understand the synergy between the helicase and the RD for RNA binding, and the contribution of ATP hydrolysis to RIG-I activation, we determined the structure of human RIG-I helicase-RD in complex with dsRNA and an ATP analogue. The helicase-RD organizes into a ring around dsRNA, capping one end, while contacting both strands using previously uncharacterized motifs to recognize dsRNA. Small-angle X-ray scattering, limited proteolysis and differential scanning fluorimetry indicate that RIG-I is in an extended and flexible conformation that compacts upon binding RNA. These results provide a detailed view of the role of helicase in dsRNA recognition, the synergy between the RD and the helicase for RNA binding and the organization of full-length RIG-I bound to dsRNA, and provide evidence of a conformational change upon RNA binding. The RIG-I helicase-RD structure is consistent with dsRNA translocation without unwinding and cooperative binding to RNA. The structure yields unprecedented insight into innate immunity and has a broader impact on other areas of biology, including RNA interference and DNA repair, which utilize homologous helicase domains within DICER and FANCM.

59 BASIC BIOLOGICAL SCIENCES↗

Supporting Crew Medical Decisions on Deep Space Missions: A Real-Time Performance Monitoring

Crewed missions into deep space will require astronauts to respond autonomously to safety- and time-critical anomalies. These and other potential issues will require the continuous monitoring and recognition of potentially subtle, but complex, anomalous data patterns. NASA continues to investigate real-time metrics for Extravehicular Operations1 and for providing feedback to improve performance2. Since it is unknown how long-term exposure to deep space will affect crew health, continuous monitoring of clinical and subclinical status is warranted. NASA is characterizing crew medical decision-support needs and identifying the recommended requirements of a Clinical Decision Support System (CDSS) within the Exploration Medical Capability Element (ExMC) of the Human Research Program3. Early detection, and continuous monitoring, of performance may provide an effective prognostic capability for assessing crew health state, and the identified requirements could be provided for an in-vehicle CDSS that acts as an assistant for delivering optimal health and performance and medical care during exploration missions. There is evidence to suggest that automatic, unobtrusive collection of keystroke features, such as alphanumeric key latencies, backspaces or typing rhythm, could be a valuable performance screening tool. Keystroke features show significant differences between control subjects and patients with overt clinical illness, such as multiple sclerosis4 and Parkinson’s disease5, as well as behavioral health and performance issues, such as mild cognitive impairment6 and depression7. This work is exploring a capability for the assessment of early task performance decline in crew as they perform daily activities. Timestamped keyboard entries are continuously collected and stored. Nominal keystroke interactions are compared to those obtained after exposure to spaceflight stressors, e.g., fatigue, to determine the methods sensitivity. This work will pave the way toward an objective detection tool that may be deployed in a spaceflight setting.

clinical↗

Lipopolysaccharide O-antigen delays plant innate immune recognition of Xylella fastidiosa

Lipopolysaccharides (LPS) are among the known pathogen-associated molecular patterns (PAMPs). LPSs are potent elicitors of PAMP-triggered immunity (PTI), and bacteria have evolved intricate mechanisms to dampen PTI. Here we demonstrate that Xylella fastidiosa (Xf), a hemibiotrophic plant pathogenic bacterium, possesses a long chain O-antigen that enables it to delay initial plant recognition, thereby allowing it to effectively skirt initial elicitation of innate immunity and establish itself in the host. Lack of the O-antigen modifies plant perception of Xf and enables elicitation of hallmarks of PTI, such as ROS production specifically in the plant xylem tissue compartment, a tissue not traditionally considered a spatial location of PTI. To explore translational applications of our findings, we demonstrate that pre-treatment of plants with Xf LPS primes grapevine defenses to confer tolerance to Xf challenge.

60 APPLIED LIFE SCIENCES↗