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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 1,423 records · Page 79
All sourcesNASA NTRSDOE OSTI
NASA NTRS2008

Expert System Control of Plant Growth in an Enclosed Space

The Expert System is an enclosed, controlled environment for growing plants, which incorporates a computerized, knowledge-based software program that is designed to capture the knowledge, experience, and problem-solving skills of one or more human experts in a particular discipline. The Expert System is trained to analyze crop/plant status, to monitor the condition of the plants and the environment, and to adjust operational parameters to optimize the plant-growth process. This system is intended to provide a way to remotely control plant growth with little or no human intervention. More specifically, the term control implies an autonomous method for detecting plant states such as health (biomass) or stress and then for recommending and implementing cultivation and/or remediation to optimize plant growth and to minimize consumption of energy and nutrients. Because of difficulties associated with delivering energy and nutrients remotely, a key feature of this Expert System is its ability to minimize this effort and to achieve optimum growth while taking into account the diverse range of environmental considerations that exist in an enclosed environment. The plant-growth environment for the Expert System could be made from a variety of structures, including a greenhouse, an underground cavern, or another enclosed chamber. Imaging equipment positioned within or around the chamber provides spatially distributed crop/plant-growth information. Sensors mounted in the chamber provide data and information pertaining to environmental conditions that could affect plant development. Lamps in the growth environment structure supply illumination, and other additional equipment in the chamber supplies essential nutrients and chemicals.

May, George↗
DOE OSTI2024

A new 181 Ta neutron resolved resonance region evaluation

A new 181 Ta neutron resolved resonance region evaluation has been performed from the thermal energy range up to approximately 2.5 keV. The R-matrix SAMMY code was used with the Reich–Moore approximation to evaluate resonance parameters from several experimental data sets. A Monte Carlo approach was used for resonance spin assignments and generating 59 small fictitious resonance levels which were shown to improve the cumulative level, Porter-Thomas, and Wigner distributions as compared to theoretical predictions. Covariance information was also generated for the entire resolved resonance region. Finally, the positive impact of the new evaluation was validated through benchmark calculations which were sensitive to the 181 Ta cross section and showed improvement in the reactivity bias for several benchmark cases.

11 NUCLEAR FUEL CYCLE AND FUEL MATERIALS↗
DOE OSTI2025

Continuing Development of the Nuclear Data Processing Code AMPX [Poster]

The ENDF/B-VIII.1 evaluation library has seen a great growth in the thermal neutron scattering sub-library. The SCALE code system has traditionally approached CE transport by assuming that the CE library on disk represented the fully expanded cumulative probability distributions, conditional on exiting angle and marginal on exiting energy. While this is a complete description of the data, it comes at the potential cost of large amounts of on-disk storage. This approach was strained by several TSL files in ENDF/B-VIII.1, such as graphite, which contained data for a large number of Bragg edges. In the fully expanded probability distributions, this was found to be a disproportionately large fraction of the SCALE CE library.

GNDS↗
NASA NTRS2011

Science of Opportunity: Heliophysics on the FASTSAT Mission and STP-S26

The FASTSAT spacecraft, which was launched on November 19, 2010 on the DoD STP-S26 mission, carries three instruments developed in joint collaboration by NASA GSFC and the US Naval Academy: PISA, TTl, and MINI_ME.I,1 As part of a rapid-development, low-cost instrument design and fabrication program, these instruments were a perfect match for FASTSAT, which was designed and built in less than one year. These instruments, while independently developed, provide a collaborative view of important processes in the upper atmosphere relating to solar and energetic particle input, atmospheric response, and ion outflow. PISA measures in-situ irregularities in electron number density, TIl provides limb measurements of the atomic oxygen temperature profile with altitude, and MINI-ME provides a unique look at ion populations by a remote sen sing technique involving neutral atom imaging. Together with other instruments and payloads on STP-S26 such as the NSF RAX mission, FalconSat-5, and NanoSail-D (launched as a tertiary payload from FASTSAT), these instruments provide a valuable "constellation of opportunity" for following the now of energy and charged and neutral particles through the upper atmosphere. Together, and for a small fraction of the price of a major mission, these spacecraft will measure the energetic electrons impacting the upper atmosphere, the ions leaving it, and the large-scale plasma and neutral response to these energy inputs. The result will be a new model for maximizing scientific return from multiple small, distributed payloads as secondary payloads on a larger launch vehicle.

Rowland, Douglas E.↗
NASA NTRS2012

Overview of the 2010 Carbonaceous Aerosols and Radiative Effects Study (CARES)

Substantial uncertainties still exist in the scientific understanding of the possible interactions between urban and natural (biogenic) emissions in the production and transformation of atmospheric aerosol and the resulting impact on climate change. The US Department of Energy (DOE) Atmospheric Radiation Measurement (ARM) program's Carbonaceous Aerosol and Radiative Effects Study (CARES) carried out in June 2010 in Central Valley, California, was a comprehensive effort designed to improve this understanding. The primary objective of the field study was to investigate the evolution of secondary organic and black carbon aerosols and their climate-related properties in the Sacramento urban plume as it was routinely transported into the forested Sierra Nevada foothills area. Urban aerosols and trace gases experienced significant physical and chemical transformations as they mixed with the reactive biogenic hydrocarbons emitted from the forest. Two heavily-instrumented ground sites - one within the Sacramento urban area and another about 40 km to the northeast in the foothills area - were set up to characterize the evolution of meteorological variables, trace gases, aerosol precursors, aerosol size, composition, and climaterelated properties in freshly polluted and "aged" urban air. On selected days, the DOE G-1 aircraft was deployed to make similar measurements upwind and across the evolving Sacramento plume in the morning and again in the afternoon. The NASA B-200 aircraft, carrying remote sensing instruments, was also deployed to characterize the vertical and horizontal distribution of aerosols and aerosol optical properties within and around the plume. This overview provides: (a) the scientific background and motivation for the study, (b) the operational and logistical information pertinent to the execution of the study, (c) an overview of key observations and initial findings from the aircraft and ground-based sampling platforms, and (d) a roadmap of planned data analyses and focused modeling efforts that will facilitate the integration of new knowledge into improved representations of key aerosol processes and properties in climate models.

aerosols↗
NASA NTRS2019

Small Satellites for NASA Earth Science

NASA’s Earth Science Division (ESD) seeks to develop a scientific understanding of Earth and its response to natural and human-induced changes. Earth is a system comprised of diverse components interacting in complex ways. Understanding Earth’s atmosphere, surface and interior, oceans and surface water, ice and snow, and life as a single connected system is necessary in order to improve our predictions of climate, weather, and natural hazards. The ESD’s Flight Program consists of a coordinated series of satellite and airborne systems providing long and short-term, global and regional observations. In addition, the Flight Program provides infrastructure for operating these missions, processing their scientific data, and distributing them on a free and open basis to researchers, operational users, and the public. The Flight Program currently has 24 operating Earth observing space missions and instruments. There are 18 more missions and instruments planned for launch over the next five years. These comprise missions recommended by the National Academies 2017 Earth Science Decadal Survey, missions and selected instruments to ensure availability of key climate data sets, operational missions to sustain the land imaging provided by the Landsat system, and small-sized competitively selected orbital and instrument missions of opportunity belonging to the Earth Venture (EV) program. The Earth Science Decadal Survey, released in early 2018, recommended four new Flight Program elements in addition to the above activities that comprise the Program of Record (POR). Small satellites (~500 kg or less) are essential components of these activities. Presently, there is an increasing use of micro and nanosatellites (or CubeSats) in constellations to support NASA ESD’s scientific objectives. These include the Cyclone Global Navigation Satellite System (CYGNSS) for observing tropical cyclone intensification and genesis factors, the Timed-Resolved Observations of Precipitation structure and storm Intensity with a Constellation of Smallsats (TROPICS) mission, and the Polar Radiant Energy in the Far InfraRed Experiment (PREFIRE) CubeSat mission. ESD small satellite initiatives like the Small Satellite Constellation Data Buy and Venture Class Launch Services (VCLS) are also underway. The Earth Science Technology Office’s (ESTO) In-Space Validation of Earth Science Technologies (InVEST) and the Venture Technology program elements have launched seven 3U and 6U CubeSat missions to validate advanced instruments and related technologies. An equivalent number of InVEST and other technology demonstration CubeSats are being prepared for launch in the next year. An overview of plans and current status including topics related to small satellite enabling activities will be presented.

Neeck, Steven P.↗
DOE OSTI2022

The DOE E3SM Model Version 2: Overview of the Physical Model and Initial Model Evaluation

Abstract This work documents version two of the Department of Energy's Energy Exascale Earth System Model (E3SM). E3SMv2 is a significant evolution from its predecessor E3SMv1, resulting in a model that is nearly twice as fast and with a simulated climate that is improved in many metrics. We describe the physical climate model in its lower horizontal resolution configuration consisting of 110 km atmosphere, 165 km land, 0.5° river routing model, and an ocean and sea ice with mesh spacing varying between 60 km in the mid‐latitudes and 30 km at the equator and poles. The model performance is evaluated with Coupled Model Intercomparison Project Phase 6 Diagnosis, Evaluation, and Characterization of Klima simulations augmented with historical simulations as well as simulations to evaluate impacts of different forcing agents. The simulated climate has many realistic features of the climate system, with notable improvements in clouds and precipitation compared to E3SMv1. E3SMv1 suffered from an excessively high equilibrium climate sensitivity (ECS) of 5.3 K. In E3SMv2, ECS is reduced to 4.0 K which is now within the plausible range based on a recent World Climate Research Program assessment. However, a number of important biases remain including a weak Atlantic Meridional Overturning Circulation, deficiencies in the characteristics and spectral distribution of tropical atmospheric variability, and a significant underestimation of the observed warming in the second half of the historical period. An analysis of single‐forcing simulations indicates that correcting the historical temperature bias would require a substantial reduction in the magnitude of the aerosol‐related forcing.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2019

Resilient Transmission Hardening Planning in a High Renewable Penetration Erra

Hardening components in transmission systems is a practice to improve system resilience against possible disturbances caused by natural disasters. In a power system with a very high penetration of renewable energy, the system hardening will be further complicated by the uncertainty and variability of renewable energy. In this paper, we study the transmission line hardening planing problem in the context of probabilistic power flows injected by the high penetration of renewable energy. We assume that the probabilistic information of renewable energy is incomplete and ambiguous and propose a data-driven approach to approximate the renewable uncertainty sets. We then extend the N - 1 security criteria to multiple simultaneous contingencies and seek to prepare a hardening plan for the worst-case scenarios. A two-stage data-driven stochastic model is formulated by considering the joint worst-case wind output distribution and transmission line contingencies. Then, we apply the Column-and-Constraints generation method to solve the proposed model. To test the effectiveness of the proposed approach, we conduct experiments on 24-bus and 118-bus test systems. We numerically show that the data-driven approach can effectively address the uncertainty ambiguity and the proposed approach can produce effective hardening plans that improve the system resilience.

Column-and-Constraint generation↗
NASA NTRS2014

New Laboratory-Based Satellite Impact Experiments for Breakup Fragment Characterization

A consortium consisting of the NASA Orbital Debris Program Office, U.S. Air Force's Space and Missile Systems Center, the Aerospace Corporation, and University of Florida is planning a series of hypervelocity impact experiments on mockup targets at the U.S. Air Force's Arnold Engineering Development Complex (AEDC) in early 2014. The target for the first experiment resembles a rocket upper stage whereas the target for the second experiment represents a typical 60‐cm/50‐kg class payload that incorporates modern spacecraft materials and components as well as exterior wrap of multi‐layer insulation and three solar panels. The projectile is designed with the maximum mass that AEDC's Range G two‐stage light gas gun can accelerate to an impact speed of 7 km/sec. The impact energy is expected to be close to 15 MJ to ensure catastrophic destruction of the target after the impact. Low density foam panels are installed inside the target chamber to slow down and soft‐catch the fragments for post‐impact processing. Diagnostic instruments, such as x‐ray and high speed optical cameras, will also be used to record the breakup process. The main goal of this "DebriSat" project is to characterize the physical properties, including size, mass, shape, and density distributions, of orbital debris that would be generated by a hypervelocity collision involving an upper stage or a modern satellite in the low Earth orbit environment. In addition, representative fragments will be selected for laboratory optical and radar measurements to allow for better interpretation of data obtained by telescope and radar observations. This paper will provide a preliminary report of the impact results and the plans to process, measure, and analyze the fragments.

Liou, J.-C.↗
DOE OSTI2022

Feedback Control Approaches for Restoration of Power Grids from Blackouts

The automated restoration of power systems with variable energy resources is a timely problem to tackle. Automated restoration advice can support operators in deciding on strategic actions to restore power grids from a blackout with a mix of conventional and renewable generation resources. To this end, this paper frames the restoration process of power grids with solar resources as a nonlinear dynamic model with algebraic constraints in discrete time which is steered by feedback control loops. We discuss two feedback-control strategies based on greedy and reinforcement learning algorithms, and contrast their performance with restoration plans generated by a mixed-integer linear program. We found that the reinforcement learning algorithm infers restoration actions faster than the greedy one. However, the tuning process of the reinforcement learning parameters is slower than for the greedy one.

machine learning↗
DOE OSTI2017

Evaluating the Performance Envelope of Axial Flow HEPA Filter Designs - 17466

Nuclear grade high efficiency particulate air (HEPA) filters are routinely employed as the final control element in confinement ventilation systems. Approximately 6000 nuclear grade HEPA filters are purchased each year for use in the Department of Energy National Nuclear Security Administration (DOE/NNSA) complex. The traditional HEPA filter utilizes a pleated filter medium of fibrous glass that has been produced in a manner analogous to manufacturing paper. Containment ventilation systems are designed to condition the air-stream (temperature, relative humidity, moisture, particle size distribution of aerosols, and aerosol concentration) to levels and conditions that fibrous glass HEPA filters can handle. Axial Flow filters can be purchased with physical separators or embossed medium to maintain proper pleat geometry. Separator-less designs use media embossed with a corrugation pattern to retain pleat geometry. At least some versions of the separator-less filter packs have demonstrated unexpected and rapid failure under slightly elevated temperature and humidity conditions. The effects of age on HEPA filter performance have been a concern for at least two decades. A combination of physical evaluation of aged filter media and a review of filter failure records resulted in Appendix C of the Nuclear Air Cleaning Handbook (NACH), Determination of HEPA Filter Life. Mississippi State University is conducting studies to more clearly identify the operating envelope of separator and separator-less designs of ASME AG-1 Section FC axial flow HEPA filters under a set of generic design basis conditions. A parallel study is also being conducted to evaluate the effects of age on fibrous glass HEPA filters. Activities described in this paper have been conducted under Institute for Clean Energy Technology (ICET's) NQA-1 nuclear quality assurance program approved by DOE-EM. Testing meets the requirements of EM Quality Assurance Program (QAP) EM-QA-001, Rev. 1, June 11, 2012 that includes the applicable requirements of NQA-1-2008/2009a and DOE Order 414.1D. (authors)

07 ISOTOPE AND RADIATION SOURCES↗
DOE OSTI2024

Communities LEAP: Microgrids 101 [Slides]

Through the Communities Local Energy Action Program (LEAP), NREL is providing technical assistance to a coalition of stakeholders from Oakridge, Oregon. The coalition includes community-based non-profit organizations, the city government, the local utility, and others. Technical assistance provides analysis and information to support Oakridge stakeholders with their goals to increase energy reliability and resilience in the community, while promoting economic development. The Grid Resilience and Microgrids Learning Session was presented to the Oakridge coalition to provide foundational technical knowledge that can support decision-making about energy-related issues in the community.

24 POWER TRANSMISSION AND DISTRIBUTION↗
DOE OSTI2026

Flexible Operation of Natural Gas Power Plants in Texas: Startup and Shutdown Durations and Nitrogen Dioxide Emissions

This dataset provides insights into historical flexible operation of natural gas power plants in Texas, with a focus on startup and shutdown events. The dataset includes tables summarizing startup and shutdown durations as well as nitrogen oxide (NOx) emission factors during these events, and compares these emission factors with those observed during all other operating phases (referred to here as “steady-state operation”). The dataset is derived using the U.S. Environmental Protection Agency (EPA)’s Clean Air Markets Program Data (CAMPD). Historical hourly data from 2015–2024, including electricity generation, heat input, and NOx emission factors, are used for natural gas combined cycle units, combustion turbine units, and steam turbine units in Texas. This work was authored by the National Laboratory of the Rockies, operated by the Alliance for Energy Innovation, LLC, for the U.S Department of Energy (DOE) under contract no. DE-AC36-08GO28308. Funding was provided by the U.S. Department of Energy as part of its Grid Modernization Laboratory Consortium, a strategic partnership between DOE and the national laboratories to bring together leading experts, technologies, and resources to collaborate on the goal of modernizing the nation’s grid. The views expressed in the dataset do not necessarily represent the views of the DOE or the U.S. Government.

24 POWER TRANSMISSION AND DISTRIBUTION↗
DOE OSTI2024

MOOSE-Based Fast Reactor Core Bowing Capabilities: Coupled Structural Mechanics – Thermal Fluids Demonstration and Related Verification Efforts

Under the U.S. Department of Energy Office of Nuclear Energy’s Advanced Modeling and Simulation (NEAMS) Program, an integrated multiphysics approach is being developed to model the core bowing phenomena important to liquid metal-cooled fast reactors. Core bowing is an important passive safety mechanism in liquid metal-cooled fast reactors and involves multiphysics effects including radiation transport, fluid flow, heat transfer, and mechanical response to temperature and flux gradients. Verification and assessment efforts continued on the Multiphysics Object Oriented Simulation Environment (MOOSE) capabilities relevant for modeling thermo-mechanical core bowing behavior. IAEA Verification Problem 4, which was started in FY23, was further examined with MOOSE capabilities to rectify discrepancies observed in previous years when compared to IAEA benchmark participant data. Meshing and postprocessing capabilities in MOOSE were also advanced by other teams and utilized this year. A thermal fluids-structural mechanical coupling demonstration has performed on 7-assemblyand 19-assembly fast reactor assembly configurations using MOOSE. Subchannel capabilities are used to calculate coolant temperature, and heat conduction capabilities calculate duct wall temperature as well as heat transfer through the inter-assembly gap. Structural mechanical capabilities then deform the mesh, accounting for contact between assemblies, according to the temperature gradients calculated by the thermal solvers. Power distributions are imposed rather than calculated to demonstrate different deformations.

22 GENERAL STUDIES OF NUCLEAR REACTORS↗
NASA NTRS1993

Instantaneous velocity field imaging instrument for supersonic reacting flows

The technical tasks conducted to develop and demonstrate a new gas velocity measurement technique for high enthalpy reacting flows is described. The technique is based on Doppler-shifted Planar Laser-induced Fluorescence (PLIF) imaging of the OH radical. The imaging approach permits, in principle, single-shot measurements of the 2-D distribution of a single velocity component in the measurement plane, and is thus a technique of choice for applications in high enthalpy transient flow facilities. In contrast to previous work in this area, the present program demonstrated an approach which modified the diagnostic technique to function under the constraints of practical flow conditions of engineering interest, rather than vice-versa. In order to accomplish the experimental demonstrations, the state-of-the-art in PLIF diagnostic techniques was advanced in several ways. Each of these tasks is described in detail and is intended to serve as a reference in supporting the transition of this new capability to the fielded PLIF instruments now installed at several national test facilities. Among the new results of general interest in LlF-based flow diagnostics, a detailed set of the first measurements of the collisional broadening and shifting behavior of OH (1,0) band transitions in H7-air combustion environments is included. Such measurements are critical in the design of a successful strategy for PLIF velocity imaging; they also relate to accurate concentration and temperature measurements, particularly in compressible flow regimes. Furthermore, the results shed new light on the fundamental relationship between broadening and energy transfer collisions in OH A(sup 2)Sigma(+)v(sup ') = 1. The first single-pulse, spectrally-resolved measurements of the output of common pulsed dye lasers were also produced during the course of this effort. As with the OH broadening measurements, these data are a significant aspect of a successful velocity imaging strategy, and also have potential implications for many other LIF measurement techniques. Our results indicated the need to modify the commercially available laser cavity in order to accommodate the constraints imposed by typical SCRAMJET combustion characteristics as well as to increase the instrument's velocity dynamic range to span an intra-image range in excess of 2 km/s. The various technical efforts were brought together in a series of experiments demonstrating the applicability of the technique in a high pressure, high temperature H2-air combustion system. The resultant images were compared with 2-D flow simulations in order to determine the accuracy of the instrument. Mean velocity imaging in flows with an axis of symmetry was demonstrated with an accuracy of +/- 50 m/s out of an intra-image dynamic range of 1600 m/s, including reversed flow. A more complex configuration amenable to single-shot imaging in flows without an axis of symmetry was also demonstrated. Limitations imposed by available equipment resulted in an accuracy of about +/- 200 m/s out of 1750 m/s in these demonstrations. Minor modifications to the present configuration were suggested to improve this performance. Each technical task is described in detail, along with significance of the results for the overall imaging velocimeter configuration. This report should allow the user community to integrate this new measurement capability in their existing instrumentation platforms.

Allen, M. G.↗
NASA NTRS1994

Auroral ionospheric signatures of the plasma sheet boundary layer in the evening sector

We report on particles and fields observed during Defense Meteorological Satellite Program (DMSP) F9 and DE 2 crossings of the polar cap/auroral oval boundary in the evening magnetic local time (MLT) sector. Season-dependent, latitudinally narrow regions of rapid, eastward plasma flows were encountered by DMSP near the poleward boundary of auroral electron precipitation. Ten DE 2 orbits exhibiting electric field spikes that drive these plasma flows were chosen for detailed analysis. The boundary region is characterized by pairs of oppositely-directed, field-aligned current sheets. The more poleward of the two current sheets is directed into the ionosphere. Within this downward current sheet, precipitating electrons either had average energies of a few hundred eV or were below polar rain flux levels. Near the transition to upward currents, DE 2 generally detected intense fluxes of accelerated electrons and weak fluxes of ions, both with average energies between 5 and 12 keV. In two instances, precipitating ions with energies greater than 5 keV spanned both current sheets. Comparisons with satellite measurements at higher altitudes suggest that the particles and fields originated in the magnetotail inside the distant reconnection region and propagated to Earth through the plasma sheet boundary layer. Auroral electrons are accelerated by parallel electric fields produced by the different pitch angle distributions of protons and electrons in this layer interacting with the near-Earth magnetic mirror. Electric field spikes driving rapid plasma flows along the poleward boundaries of intense, keV electron precipitation represent ionospheric responses to the field-aligned currents and conductivity gradients. The generation of field-aligned currents in the boundary layer may be understood qualitatively as resulting from the different rates of earthward drift for electrons and protons in the magnetotail's current sheet.

Burke, W. J.↗
DOE OSTI2020

Comparing event generator predictions and ab-initio calculations of $\nu$-$^{12}$C neutral current quasi-elastic scattering at 1 GeV

The measurement of neutrino oscillations and exotic physics searches are important parts of the physics program in the near future, with new state-of-the-art experiments planned within the next decade. Future and modern experiments in these fields will make use of nuclear targets. Event Generators (EGs) are software used in the analysis of neutrino oscillation experiments. EGs use to predict kinematic observables for a range of neutrino energies. These simulations may lack physics captured by more rigorous theoretical calculations. This work compares EG performance to nuclear theory calculations by comparing observables generated in the two frameworks. We provide a common set of definitions between theory and experiment and assess the physics contained in EG simulations. Neutral current quasi-elastic (NCQE) scattering events for neutrinos and anti-neutrinos on a $^{12}$C target are simulated with a specific EG, NEUT, used by the T2K experiment for its analysis. The simulated cross sections are compared to analytic calculations from nuclear theory within the factorization scheme. We compare the NEUT implementation of two different models on nuclear spectral functions: the Relativistic Fermi Gas (RFG) and the correlated basis spectral function (CBF) to analytic calculations of the same models in the factorization scheme. For both nuclear physics models, we compare the appearance of features in the distributions relevant to experimental analyses. Qualitatively, the shape of the simulated distribution is similar to the one obtained through theory calculations; however, there are some discrepancies between the theory calculations and the NEUT simulation. While the EG simulations and analytic calculations with the same model of nuclear dynamics show similar overall features, there are still differences between the two.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗
NASA NTRS2002

Spatial distribution and yield of DNA double-strand breaks induced by 3-7 MeV helium ions in human fibroblasts

Accelerated helium ions with mean energies at the target location of 3-7 MeV were used to simulate alpha-particle radiation from radon daughters. The experimental setup and calibration procedure allowed determination of the helium-ion energy distribution and dose in the nuclei of irradiated cells. Using this system, the induction of DNA double-strand breaks and their spatial distributions along DNA were studied in irradiated human fibroblasts. It was found that the apparent number of double-strand breaks as measured by a standard pulsed-field gel assay (FAR assay) decreased with increasing LET in the range 67-120 keV/microm (corresponding to the energy of 7-3 MeV). On the other hand, the generation of small and intermediate-size DNA fragments (0.1-100 kbp) increased with LET, indicating an increased intratrack long-range clustering of breaks. The fragment size distribution was measured in several size classes down to the smallest class of 0.1-2 kbp. When the clustering was taken into account, the actual number of DNA double-strand breaks (separated by at least 0.1 kbp) could be calculated and was found to be in the range 0.010-0.012 breaks/Mbp Gy(-1). This is two- to threefold higher than the apparent yield obtained by the FAR assay. The measured yield of double-strand breaks as a function of LET is compared with theoretical Monte Carlo calculations that simulate the track structure of energy depositions from helium ions as they interact with the 30-nm chromatin fiber. When the calculation is performed to include fragments larger than 0.1 kbp (to correspond to the experimental measurements), there is good agreement between experiment and theory.

NASA Program Biomedical Research and Countermeasur↗