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DOE OSTI2014

Multiplexed Digital mRNA Profiling of the Inflammatory Response in the West Nile Swiss Webster Mouse Model

Background and purpose: The ability to track changes in gene expression following viral infection is paramount to understanding viral pathogenesis. This study was undertaken to evaluate the nCounter, a high throughput digital gene expression system, as a means to better understand West Nile virus (WNV) dissemination and the inflammatory response against WNV in the outbred Swiss Webster (SW) mouse model over the course of infection. Methodology: The nCounter Mouse Inflammation gene expression kit containing 179 inflammation related genes was used to analyze gene expression changes in multiple tissues over a nine day course of infection in SW mice following intraperitoneal injection with WNV. Protein expression levels for a subset of these cytokine/chemokine genes were determined using a multiplex protein detection system (BioPlex) and comparisons of protein/RNA expression levels made. Results: Expression analysis of spleen, lung, liver, kidney and brain of SW mice infected with WNV revealed that Cxcl10 and Il12b are differentially expressed in all tissues tested except kidney. Data stratification of positively confirmed infected (WNV (+)) versus non-infected (WNV (2) tissues allowed differentiation of the systemic inflammatory gene response from tissue specific responses arising from WNV infection. Significant (p,0.05) decrease in C3ar1 was found in WNV (2) spleen. Il23a was significantly upregulated, while Il10rb was down-regulated in WNV (2) lung. Il3 and Mbl2 were down-regulated in WNV (2) liver. In WNV (+) livers, Stat1, Tlr2, chemokines Cxcl1, Cxcl3, Cxcl9, Cxcl10, cytokines Il6, Il18, cytokine-related gene Il1r and cytokine agonist Ilrn were significantly upregulated. In WNV (2) brain tissues, Csf2 and Cxcl10 were significantly upregulated. Similar gene and protein expression kinetics were found for Ccl2, Ccl3, Ccl4 and Ccl5 and correlated with the presence of infectious virus. In summary, the utility of the nCounter platform for rapid identification of gene expression changes in SW mice associated with WNV infection was demonstrated.

54 ENVIRONMENTAL SCIENCES↗

NASA NTRS2000

Solar Powered Aircraft, Photovoltaic Array/Battery System Tabletop Demonstration: Design and Operation Manual

A system was constructed to demonstrate the power system operation of a solar powered aircraft. The system consists of a photovoltaic (PV) array, a charge controller, a battery, an electric motor and propeller. The system collects energy from the PV array and either utilizes this energy to operate an electric motor or stores it in a rechargeable battery for future use. The system has a control panel which displays the output of the array and battery as well as the total current going to the electric motor. The control panel also has a means for adjusting the output to the motor to control its speed. The entire system is regulated around 12 VDC.

Colozza, Anthony J.↗

NASA NTRS2016

Engineering Design Tools for Shape Memory Alloy Actuators: CASMART Collaborative Best Practices and Case Studies

The primary goal of the Consortium for the Advancement of Shape Memory Alloy Research and Technology (CASMART) is to enable the design of revolutionary applications based on shape memory alloy (SMA) technology. In order to help realize this goal and reduce the development time and required experience for the fabrication of SMA actuation systems, several modeling tools have been developed for common actuator types and are discussed herein along with case studies, which highlight the capabilities and limitations of these tools. Due to their ability to sustain high stresses and recover large deformations, SMAs have many potential applications as reliable, lightweight, solid-state actuators. Their advantage over classical actuators can also be further improved when the actuator geometry is modified to fit the specific application. In this paper, three common actuator designs are studied: wires, which are lightweight, low-profile, and easily implemented; springs, which offer actuation strokes upwards of 200 at reduced mechanical loads; and torque tubes, which can provide large actuation forces in small volumes and develop a repeatable zero-load actuation response (known as the two-way shape memory effect). The modeling frameworks, which have been implemented in the design tools, are developed for each of these frequently used SMA actuator types. In order to demonstrate the versatility and flexibility of the presented design tools, as well as validate their modeling framework, several design challenges were completed. These case studies include the design and development of an active hinge for the deployment of a solar array or foldable space structure, an adaptive solar array deployment and positioning system, a passive air temperature controller for regulation flow temperatures inside of a jet engine, and a redesign of the Corvette active hatch, which allows for pressure equalization of the car interior. For each of the presented case studies, a prototype or proof-of-concept was fabricated and the experimental results and lessons learned are discussed. This analysis presents a collection of CASMART collaborative best practices in order to allow readers to utilize the available design tools and understand their modeling principles. These design tools, which are based on engineering models, can provide first-order optimal designs and are a basic and efficient method for either demonstrating design feasibility or refining design parameters. Although the design and integration of an SMA-based actuation system always requires application- and environment-specific engineering considerations, common modeling tools can significantly reduce the investment required for actuation system development and provide valuable engineering insight.

Design tools↗

NASA NTRSDate not supplied

Icing Physics Studies Using the 3D SIDRM Test Article: Ice Crystal Icing Analysis

In-flight icing is an important safety issue and is a factor that affects aircraft design and performance. Newer regulations are driving a need for improvements in airframe and engine icing simulation capability. Experimental data is required for the development of icing physics models and simulation validation. This paper presents the analysis of the ice crystal icing data subset from tests conducted in 2022 at the NASA Icing Research Tunnel that studied both supercooled liquid and ice-crystal icing. The test article that was utilized replicated 3D geometrical features of an inter-compressor duct and strut region of a turbofan engine. The surfaces of the Simulated Inter-compressor Duct Research Model (SIDRM) can be heated to simulate the warm surfaces of the turbofan inter-compressor duct. The test article is instrumented with pressure taps, heaters, heat flux gauges, and thermocouples, while a 3D laser scanner, cameras, and a weighing scale to measure ice mass were utilized to characterize the icing behavior. The aim of these tests was to generate ice accretions on the SIDRM test article under well-characterized icing conditions. To that end, ice crystal cloud characterization tests were conducted that provided cloud property values at the test section. The ice crystal accretion tests investigated how different test condition parameters impacted ice accretion size, location, and characteristics (such as physical attributes). A key finding was that a sufficiently high surface heat flux at the leading edge can provide a continuous supply of liquid melt to critical accretion zones downstream, resulting in elevated ice mass accretions. Another key finding was that warmer heater settings in critical accretion zones can suppress ice accretion. These findings are consistent with icing behavior observed in full-scale engine icing tests conducted previously at the NASA Glenn Research Center. Test runs investigating the contribution of liquid melt generated at the leading edge found that the melt can be transported downstream to critical accretion regions in two forms, via runback and splash. The form of melt supplied downstream affected accretion behavior and features. Sharkteeth-shaped ice accretions were generated at certain test conditions, a physical attribute that has been observed in full-scale engine icing tests. The ice accretion data will be used to develop and validate 3D computational engine icing tools, such as GlennICE, that predictively assesses the onset and growth of ice. One of the goals of the sponsoring NASA project is to develop simulation models and tools that can assist in the design and certification of engines for flight in icing conditions in a cost‑effective way.

ice crystal icing↗

NASA NTRSDate not supplied

Icing Physics Studies Using the 3D SIDRM Test Article: Ice Crystal Icing Analysis

ice crystal icing↗

DOE OSTI2022

Performance Assessment of High-Efficiency Refrigerated Display Cases with Low-GWP Refrigerants: Preprint

As California policy makers enforce new regulations such as SB 1383 to achieve state greenhouse gas (GHG) reduction goals, it will be critical to understand the energy efficiency implications of low global warming potential (GWP) refrigerants in commercial refrigeration applications. The goal of this research project, funded by Commonwealth Edison was to assess the energy performance of two high efficiency medium-temperature, self-contained, reach-in display cases that utilized natural refrigerant propane (R-290), and hydrofluorocarbon (HFC) drop-in hydrofluoroolefin R-513a, respectively. Performance of these display cases were compared to a baseline fixture using HFC (R-134a) under equal conditions in a controlled environment chamber. These display cases were selected due to their widespread use in convenience stores, and small supermarkets. The high efficiency cases were equipped with energy efficient lighting, improved panel insulation, high efficiency evaporator and condenser fan motors, and high effectiveness heat exchangers. The test method used in this project was foundationally inspired by the ANSI/ASHRAE 72-2018 standard method to evaluate each case under equivalent conditions. However, minor modifications to this methodology were implemented to better represent more realistic operation of the units. While maintaining target product temperature, the energy efficient fixtures containing R-290 and R-513a consumed 61.8% to 32.6% less energy, respectively, than a baseline case using R-134a refrigerant. The daily energy consumption of the R-290, R-513a, and baseline display cases at the upper limit of environmental conditions was 4.30, 7.59, and 11.26 kWh/day, respectively.

alternative refrigerants↗

DOE OSTI2022

Performance Assessment of High-Efficiency Refrigerated Display Cases With Low Global Warming Potential Refrigerants

As California policy makers enforce new regulations such as SB 1383 to achieve state greenhouse gas reduction goals, it will be critical to understand the energy efficiency implications of low global warming potential (GWP) refrigerants in commercial refrigeration applications. The goal of this research project, funded by Commonwealth Edison, was to assess the energy performance of two high-efficiency medium-temperature, self-contained, reach-in display cases that utilized natural refrigerant propane (R-290, GWP = 3), and hydrofluorocarbon (HFC) drop-in hydrofluoroolefin R-513A (GWP = 573). Performance of these display cases was compared to a baseline fixture using HFC (R-134A, GWP = 1301) under equal conditions in a controlled environment chamber. These display cases were selected due to their widespread use in convenience stores and small supermarkets. The high-efficiency cases were equipped with energy-efficient lighting, improved panel insulation, high-efficiency evaporator and condenser fan motors, and high-effectiveness heat exchangers. The test method used in this project was foundationally inspired by the ANSI/ASHRAE 72-2018 standard method to evaluate each case under equivalent conditions (ASHRAE 2018). However, minor modifications to this methodology were implemented to better represent more realistic operation of the units. While maintaining equivalent target product temperature, the energy-efficient fixtures A (containing R-290, improved insulation, heat exchangers with a higher overall heat transfer coefficient (higher UA), and more efficient lighting and fan motors) and B (containing R-513A, improved fans, and higher UA-heat exchangers, and more efficient lighting and fan motors) consumed 61.8% to 32.6% less energy, respectively, than a baseline case using R-134A refrigerant. The daily energy consumption of the R-290, R-513A, and baseline display cases at the upper limit of environmental conditions was 4.30, 7.59, and 11.26 kWh/day, respectively.

alternative refrigerants↗

DOE OSTI2018

Application of multiplexed ion mobility spectrometry towards the identification of host protein signatures of treatment effect in pulmonary tuberculosis

Rationale: The monitoring of TB treatments in clinical practice and clinical trials relies on traditional sputum-based culture status indicators at specific time points. Accurate, predictive, blood-based protein markers would provide a simpler and more informative view of patient health and response to treatment. Objective: We utilized sensitive, high throughput multiplexed ion mobility-mass spectrometry (IM-MS) to characterize the serum proteome of TB patients at the start of and at 8 weeks of rifamycin-based treatment. We sought to identify treatment specific signatures within patients as well as correlate the proteome signatures to various clinical markers of treatment efficacy. Methods: Serum samples were collected from 289 subjects enrolled in CDC TB Trials Consortium Study 29 at time of enrollment and at the end of the intensive phase (after 40 doses of TB treatment). Serum proteins were immunoafinity-depleted of high abundant components, digested to peptides and analyzed for data acquisition utilizing a unique liquid chromatography IM-MS platform (LC-IM-MS). Linear mixed models were utilized to identify serum protein changes in the host response to antibiotic treatment as well as correlations with culture status end points. Results: A total of 10,137 peptides corresponding to 872 proteins were identified, quantified, and used for statistical analysis across the longitudinal patient cohort. In response to TB treatment, 244 proteins were sig- ni?cantly altered. Pathway/network comparisons helped visualize the interconnected proteins, identifying up regulated (lipid transport, coagulation cascade, endopeptidase activity) and down regulated (acute phase) processes and pathways in addition to other cross regulated networks (inflammation, cell adhesion, extracellular matrix). Detection of possible lung injury serum proteins such as HPSE, significantly downregulated upon treatment. Analyses of microbiologic data over time identified a core set of serum proteins (TTHY, AFAM, CRP, RET4, SAA1, PGRP2) which change in response to treatment and also strongly correlate with culture status. A similar set of proteins at baseline were found to be predictive of week 6 and 8 culture status. Conclusion: A comprehensive host serum protein dataset reflective of TB treatment effect is defined. A repeating set of serum proteins (TTHY, AFAM, CRP, RET4, SAA1, PGRP2, among others) were found to change significantly in response to treatment, to strongly correlate with culture status, and at baseline to be predictive of future culture conversion. If validated in cohorts with long term follow-up to capture failure and relapse of TB, these protein markers could be developed for monitoring of treatment in clinical trials and in patient care.

Kedia, Komal↗

DOE OSTI2020

Generation of ionic liquid tolerant Pseudomonas putida KT2440 strains via adaptive laboratory evolution

Although the use of ionic liquids (ILs) for the pretreatment of lignocellulosic biomass has been limited due to high costs, recent efforts to develop low-cost protic ILs show promise for achieving cost-effectiveness for biorefineries. However, an additional challenge remains in that ILs present in biomass hydrolysates are toxic to most microbial hosts, resulting in poor growth phenotypes. To address this issue, we applied an adaptive laboratory evolution (ALE) approach for tolerizing Pseudomonas putida KT2440, an industrially relevant bacterial host, to two low-cost ILs (triethanolammonium acetate [TEOH][OAc] and triethylammonium hydrogen sulfate [TEA][HS]). After continuous cultivations with gradually increased IL levels, we obtained evolved strains showing significant improvements in their growth performance under high concentrations of the ILs (maximum 4% [TEOH][OAc] and 8% [TEA][HS], in w/v) at which the wild-type strain cannot grow. Sequencing of evolved strains revealed multiple regions where mutations were associated with improved performance in minimal media conditions (relA, gacS, oprB/PP_1446, fleQ, tktA, and uvrY/PP_4100) and in IL-specific conditions (PP_5350, PP_4929/emrE, oprD, and PP_5324). We further validated the causality of the PP_5350 and emrE genes for improved IL tolerance via reverse engineering and transcriptomic analysis. Here, a common mutation in the PP_5350 gene, encoding a RpiR family transcriptional regulator, was shown to significantly upregulate the glyoxylate cycle for efficient acetate catabolism. In addition, it was suggested that the emrE gene encodes an efflux pump which can export [TEA][HS]. Finally, the cultivation of two of the best performing evolved strains with IL-treated biomass hydrolysates demonstrated their considerable potential to be used as platform strains. Taken as a whole, this work provides strains for utilization of IL-treated biomass and a mechanistic understanding that could be further leveraged to develop efficient microbial bioprocesses.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

DOE OSTI2020

Proteomic assessment of serum biomarkers of longevity in older men

The biological bases of longevity are not well understood, and there are limited biomarkers for the prediction of long life. We used a high-throughput, discovery-based proteomics approach to identify serum peptides and proteins that were associated with the attainment of longevity in a longitudinal study of community-dwelling men age ≥65 years. Baseline serum in 1196 men were analyzed using liquid chromatography–ion mobility–mass spectrometry, and lifespan was determined during ~12 years of follow-up. Men who achieved longevity (≥90% expected survival) were compared to those who died earlier. Rigorous statistical methods that controlled for false positivity were utilized to identify 25 proteins that were associated with longevity. All these proteins were in lower abundance in long-lived men and included a variety involved in inflammation or complement activation. Lower levels of longevity-associated proteins were also associated with better health status, but as time to death shortened, levels of these proteins increased. Pathway analyses implicated a number of compounds as important upstream regulators of the proteins and implicated shared networks that underlie the observed associations with longevity. Overall, these results suggest that complex pathways, prominently including inflammation, are linked to the likelihood of attaining longevity. This work may serve to identify novel biomarkers for longevity and to understand the biology underlying lifespan.

60 APPLIED LIFE SCIENCES↗

DOE OSTI2021

Joint Work Plan for DPC Filler Testing

The US generates approximately 2,000 Metric Tons of Heavy Metal (MTHM) of commercial spent fuel (SNF) each year and currently stores ~ 85,000 MTHM of commercial SNF at 70+ reactor sites, the disposal of which is the responsibility of the US Department of Energy (DOE). SNF is initially stored in spent nuclear fueld pools (SFPs). SFPs were initially constructed by US utilities for temporary fuel storage, but with no final disposal pathway available, SFPs are reaching capacity. To allow continued operation of the nation's commercial nuclear reactor fleet, utilities started transferring SNF from SFPs (wet storage) to dry cask storage systems, typically using dual-purpose (storage and transportation) canisters (DPCs). And while DPCs were designed, licensed and loaded to meet Nuclear Regulatory Commission (NRC) requirements that preclude the possibility of a critical event during SNF storage and transport, they were not designed or loaded to preclude the possibility of a criticality event during the regulated post-closure period following disposal, which could be up to 1,000,000 years (Price, 2019). DPC filler option criteria are detailed and materials that exhibit these attributes are explored. This document is an update of the SNL Joint Workplan on Filler Investigations for DPCs.

11 NUCLEAR FUEL CYCLE AND FUEL MATERIALS↗

NASA NTRS2010

A Powerful Toolkit for Synthetic Biology: Over 3.8 Billion Years of Evolution

The combination of evolutionary with engineering principles will enhance synthetic biology. Conversely, synthetic biology has the potential to enrich evolutionary biology by explaining why some adaptive space is empty, on Earth or elsewhere. Synthetic biology, the design and construction of artificial biological systems, substitutes bio-engineering for evolution, which is seen as an obstacle. But because evolution has produced the complexity and diversity of life, it provides a proven toolkit of genetic materials and principles available to synthetic biology. Evolution operates on the population level, with the populations composed of unique individuals that are historical entities. The source of genetic novelty includes mutation, gene regulation, sex, symbiosis, and interspecies gene transfer. At a phenotypic level, variation derives from regulatory control, replication and diversification of components, compartmentalization, sexual selection and speciation, among others. Variation is limited by physical constraints such as diffusion, and chemical constraints such as reaction rates and membrane fluidity. While some of these tools of evolution are currently in use in synthetic biology, all ought to be examined for utility. A hybrid approach of synthetic biology coupled with fine-tuning through evolution is suggested

Rothschild, Lynn J.↗

NASA NTRS2022

Vision-Based Precision Approach and Landing for Advanced Air Mobility

Advanced Air Mobility (AAM) aircraft require perception systems for precision approach and landing systems (PALS) in urban, suburban, rural, and regional environments. The current state-of-the-art methods approved for automated approach and landing will be difficult to utilize in support of AAM operational concepts. However, there are technology and systems from other applications and lower-TRL research that use vision, IR, radar, and GPS methods to provide baseline perception and sensing requirements for AAM aircraft approach and landing. This paper focuses on vision-based PAL to demonstrate a closed-loop baseline controller while adhering to the Federal Aviation Administration requirements and regulations. The coplanar algorithm determines pose estimation, which feeds into an Extended Kalman filter. Combining IMU with vision creates a sensor fusion navigation solution for GPS-denied environments. The state estimate leads to glideslope and localizer error computations, which will be pertinent for designing and deriving guidance laws and control laws for AAM PALS. The IMU and vision navigation solution provides promising simulation results for AAM PALS, and higher fidelity simulations will include computer graphics rendering and feature correspondence.

Evan Kawamura↗

DOE OSTI2016

Model-Based Dead Time Optimization for Voltage-Source Converters Utilizing Silicon Carbide Semiconductors

Dead time significantly affects the reliability, power quality, and efficiency of voltage-source converters. For silicon carbide (SiC) devices, considering the high sensitivity of turn-off time to the operating conditions (> 5× difference between light load and full load) and characteristics of inductive loads (> 2× difference between motor load and inductor), as well as large additional energy loss induced by the freewheeling diode conduction during the superfluous dead time (~15% of the switching loss), then the traditional fixed dead time setting becomes inappropriate. This paper introduces an approach to adaptively regulate the dead time considering the current operating condition and load characteristics via synthesizing online monitored turn-off switching parameters in the microcontroller with an embedded preset optimization model. Here, based on a buck converter built with 1200-V SiC MOSFETs, the experimental results show that the proposed method is able to ensure reliability and reduce power loss by 12% at full load and 18.2% at light load (8% of the full load in this case study).

30 DIRECT ENERGY CONVERSION↗

DOE OSTI2022

Hierarchical Control of Utility-Scale Solar PV Plants for Mitigation of Generation Variability and Ancillary Service Provision

This paper presents a hierarchical control system to mitigate the variability of solar photovoltaic (PV) power plant and provide ancillary services to the electric grid without the need for additional non-solar resources. With coordinated management of each inverter in the system, the control system commands the power plant to proactively curtail a small fraction of its instantaneous maximum power potential, which gives the plant enough headroom to ramp up production from the overall power plant, for a service such as regulation reserve. This control system is practical for continuously changing cloud cover conditions in partially cloudy days. A case study from a site in Hawaii with one-second resolution solar irradiance data is used to verify the efficacy of the proposed control system. The proposed control algorithm is subsequently compared with the alternative control technology from the literature, the grouping control algorithm; the results show that the proposed hierarchical control system is over 10 times more effective in reducing generator mileage to support power fluctuations from solar PV power plants.

14 SOLAR ENERGY↗

DOE OSTI2017

Computer security approach for Rolls-Royce spinline{sup R} safety platform - 227

The number of digital assets at commercial nuclear power plants is significant and will likely continue to increase with time. As a consequence, these plants have become potential targets for cyber-attacks. Around the world, nuclear safety authorities and national Computer and Information Security Agencies are implementing stricter regulations and inspections for critical systems in nuclear plants. There is a particular focus on instrumentation and control systems as they implement key nuclear safety functions. Rolls-Royce follows a strict computer and information security program based on general and nuclear specific norms, Rolls-Royce group processes, and proven technologies. The Spinline digital safety platform has been designed specifically for nuclear applications and features a secure development and operational environment that provides barriers against unauthorized modifications and implements design requirements promoting integrity and reliability during operation and maintenance. The Rolls-Royce secure development environment provides layers of protection utilizing physical and environmental security, human resource security, and information technology systems access control. The Spinline platform strengthens security functions by providing protection against unauthorized, unintended, and unsafe modifications to the system. The Spinline platform has design features promote integrity and reliability during operation and maintenance in the event of inadvertent operator actions or undesirable behavior of connected equipment. The Spinline technology and these computer security processes are based in a wide spectrum of international standards that were accepted as providing a secure development and operating environment by the United States Nuclear Regulatory Commission in 2014. (authors)

22 GENERAL STUDIES OF NUCLEAR REACTORS↗

DOE OSTI2022

Grid impact analysis using controller-hardware-in-the-loop for high-power vehicle charging stations

A controller-hardware-in-the-loop (CHIL) architecture for the evaluation of grid impacts arising from high-power vehicle charging stations is presented in this paper. Unlike simulation-based studies, the proposed method can be used to capture the interactions of the grid and the charging load along with charger controllers in real time. The proposed method can be used to evaluate the impact of charging load on the grid in terms of voltage variations and line congestion. The proposed CHIL platform allows for de-risking the vehicle charging station deployment by simulating the complex interactions among all the components of a vehicle charging station - i.e., the grid, vehicle, and charger controller - in a realistic manner before using the charging station in a grid. Further, the proposed CHIL approach can be used to evaluate the voltage regulation causalities of the vehicle charging station. Experimental results are presented in the paper to illustrate the applicability of the proposed method in a laboratory environment.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

DOE OSTI2013

Enabling Secure, Scalable Microgrids with High Penetration Renewables

In the first section, ac and dc technologies are compared highlighting their advantages and disadvantages. Since ac and dc systems have both evolved significantly since their introduction in the mid and latter parts of the 19th century, many of the early advantages of ac systems no longer exist or are of less importance today. Consequently, it is useful to provide a brief historical perspective on the evolution of both ac and dc power systems. As in the dc case, there are many potential modes of operation and control strategies for the given system. In ac systems, the situation is more complex since it is necessary to regulate both the amplitude and the frequency of the ac voltage. In the third section, the techniques of controlling and analyzing the stability of ac systems is reviewed.

30 DIRECT ENERGY CONVERSION↗