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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 1,423 records · Page 79

Investigation of evolution-related aspects of bacterial rhodopsins

We have investigated evolution-related aspects of bacterial rhodopsins, the unique retinal-based energy transducing systems of halophilic archae. The approach was to describe both structural and functional aspects: the structure by sequencing genes to explore which regions are conserved, and the function by comparing proton and chloride transport in the closely related systems, bacteriorhodopsin and halorhodopsin, respectively. In the latter, we have made a good start toward the ultimate goal of separating the attributes of the general principles of retinal-based ionic pumps from those of the specific ion specificities, by determining the thermodynamics of the internal steps of the protein-mediated active transport process, as well as some of the intraprotein ion-transfer steps. Our present emphasis is on continuing to acquire the tools for studying what distinguishes proton transport from chloride transport. We consider it important, therefore, that we have been able to provide firm mathematical grounds for the kinetics analyses which underlies these studies. Our molecular biological studies have received a great boost from the expression vector for the bop gene based on a halobacterial plasmid, that we recently developed.

Source record↗

Discrimination of Single Base Pair Differences Among Individual DNA Molecules Using a Nanopore

The protein toxin alpha-hemolysin form nanometer scale channels across lipid membranes. Our lab uses a single channel in an artificial lipid bilayer in a patch clamp device to capture and examine individual DNA molecules. This nanopore detector used with a support vector machine (SVM) can analyze DNA hairpin molecules on the millisecond time scale. We distinguish duplex stem length, base pair mismatches, loop length, and single base pair differences. The residual current fluxes also reveal structural molecular dynamics elements. DNA end-fraying (terminal base pair dissociation) can be observed as near full blockades, or spikes, in current. This technique can be used to investigate other biological processes dependent on DNA end-fraying, such as the processing of HIV DNA by HIV integrase.

Vercoutere, Wenonah↗

Highly Efficient Vector-Inversion Pulse Generators

Improved transmission-line pulse generators of the vector-inversion type are being developed as lightweight sources of pulsed high voltage for diverse applications, including spacecraft thrusters, portable x-ray imaging systems, impulse radar systems, and corona-discharge systems for sterilizing gases. In this development, more than the customary attention is paid to principles of operation and details of construction so as to the maximize the efficiency of the pulse-generation process while minimizing the sizes of components. An important element of this approach is segmenting a pulse generator in such a manner that the electric field in each segment is always below the threshold for electrical breakdown. One design of particular interest, a complete description of which was not available at the time of writing this article, involves two parallel-plate transmission lines that are wound on a mandrel, share a common conductor, and are switched in such a manner that the pulse generator is divided into a "fast" and a "slow" section. A major innovation in this design is the addition of ferrite to the "slow" section to reduce the size of the mandrel needed for a given efficiency.

Rose, Franklin↗

Document Classification Techniques for Aviation Letters of Agreement

Often when working with technical documents, it is helpful to classify them into specific categories. In this paper, we conduct a thorough review of natural language processing techniques to perform this classification task on Letters of Agreement (LOAs), technical aviation documents outlining rules for utilizing US airspace. We evaluate multiple techniques, including Transfer Learning, for representing the text in the documents as embeddings: unigram and bigram Term Frequency Inverse Document Frequency (TFIDF), Word2Vec, Doc2Vec, GloVe and RoBERTa. We investigate a wide range of classification models: K-Nearest Neighbors, Random Forest, Support Vector Machines (SVM), Logistic Regression, Naive Bayes, Feed-Forward Neural Network, Convolutional Neural Networks (CNNs) and Long-Short Term Memory (LSTM). By comparing the different methods, we found the best overall approach for our task was to use unigram TFIDF representations with SVM while also gaining insight into how the other methodologies performed on a small technical datasets.

Aayushi Batra↗

Efficient track-before detect algorithm with minimal prior knowledge

Apparatus, system and method for tracking an image target in a system, wherein a system receives an image comprising a plurality of pixels. The received image is processed via a plurality of different recursive motion model kernels in parallel to provide a plurality of kernel outputs, wherein each of the motion model kernels may include a respective pixel mask. Per-pixel energy is estimated of at least some of the plurality of kernel outputs. Velocity of at least one of the image pixels may also be estimated by generating a directional energy vector for each motion model kernel. The per-pixel energy and velocity estimates are fused to produce a fused estimate representing at least some of the motion model kernels for the image.

42 ENGINEERING↗

Identification of mosquito proteins that differentially interact with alphavirus nonstructural protein 3, a determinant of vector specificity

Chikungunya virus (CHIKV) and the closely related onyong-nyong virus (ONNV) are arthritogenic arboviruses that have caused significant, often debilitating, disease in millions of people. However, despite their kinship, they are vectored by different mosquito subfamilies that diverged 180 million years ago (anopheline versus culicine subfamilies). Previous work indicated that the nonstructural protein 3 (nsP3) of these alphaviruses was partially responsible for this vector specificity. To better understand the cellular components controlling alphavirus vector specificity, a cell culture model system of the anopheline restriction of CHIKV was developed along with a protein expression strategy. Mosquito proteins that differentially interacted with CHIKV nsP3 or ONNV nsP3 were identified. Six proteins were identified that specifically bound ONNV nsP3, ten that bound CHIKV nsP3 and eight that interacted with both. In addition to identifying novel factors that may play a role in virus/vector processing, these lists included host proteins that have been previously implicated as contributing to alphavirus replication.

Byers, Nathaniel M. (ORCID:0000000157725940)↗

A wide bandwidth electrostatic field sensor for lightning research

Data obtained from UHF Radar observation of direct-lightning strikes to the NASA F-106B airplane have indicated that most of the 690 strikes acquired during direct-strike lightning tests were triggered by the aircraft. As an aid in understanding the triggered lightning process, a wide bandwidth electric field measuring system was designed for the F-106B by implementing a clamped-detection signal processing concept originated at the Air Force Cambridge Research Lab in 1953. The detection scheme combines the signals from complementary stator pairs clamped to zero volts at the exact moment when each stator pair is maximally shielded by the rotor, a process that restores the dc level lost by the charge amplifier. The new system was implemented with four shutter-type field mills located at strategic points on the airplane. The bandwidth of the new system was determined in the laboratory to be from dc to over 100 Hz, whereas past designs had upper limits of 10 Hz to 100 Hz. To obtain the undisturbed electric field vector and total aircraft charge, the airborne field mill system is calibrated by using techniques involving results from ground and flight calibrations of the F-106B, laboratory tests of a metallized model, and a finite-difference time-domain electromagnetic computer code.

Zaepfel, K. P.↗

Preliminary Orbit Determination System (PODS) for Tracking and Data Relay Satellite System (TDRSS)-tracked target Spacecraft using the homotopy continuation method

The Preliminary Orbit Determination System (PODS) provides early orbit determination capability in the Trajectory Computation and Orbital Products System (TCOPS) for a Tracking and Data Relay Satellite System (TDRSS)-tracked spacecraft. PODS computes a set of orbit states from an a priori estimate and six tracking measurements, consisting of any combination of TDRSS range and Doppler tracking measurements. PODS uses the homotopy continuation method to solve a set of nonlinear equations, and it is particularly effective for the case when the a priori estimate is not well known. Since range and Doppler measurements produce multiple states in PODS, a screening technique selects the desired state. PODS is executed in the TCOPS environment and can directly access all operational data sets. At the completion of the preliminary orbit determination, the PODS-generated state, along with additional tracking measurements, can be directly input to the differential correction (DC) process to generate an improved state. To validate the computational and operational capabilities of PODS, tests were performed using simulated TDRSS tracking measurements for the Cosmic Background Explorer (COBE) satellite and using real TDRSS measurements for the Earth Radiation Budget Satellite (ERBS) and the Solar Mesosphere Explorer (SME) spacecraft. The effects of various measurement combinations, varying arc lengths, and levels of degradation of the a priori state vector on the PODS solutions were considered.

Kirschner, S. M.↗

A further study of cumulus interactions and mergers - Three-dimensional simulations with trajectory analysis

Cumulus processes involved in the interaction and merging of clouds under the influence of different imposed conditions (including large-scale lifting forcing, environmental wind shear, and cloud microphysical processes) were studied using simulations with a three-dimensional model. The design of the study was to generate several convective clouds randomly inside the model domain, and then to observe and analyze the interactions and merging between the simulated clouds. Ten merged clouds were identified. Seven of these, each involving two previously separated clouds, generally lie along a line parallel to the initial environmental wind shear vector, while one (also a two-cloud system) lies along a line perpendicular to the wind shear vector prior to merging. The remaining two merging systems involve three parent clouds each; they are a combination of parallel and perpendicular cells. The merging mechanisms associated with three-cloud merging cases are studied by examining the temperature, pressure, and wind fields prior to, during, and following the merging.

Tao, Wei-Kuo↗

Enhanced Labeling Techniques to Study the Cytoskeleton During Root Growth and Gravitropism

Gravity effects the growth and development of all living organisms. One of the most obvious manifestations of gravity's effects on biological systems lies in the ability of plants to direct their growth along a path that is dictated by the gravity vector (called gravitropism). When positioned horizontally, in florescence stems and hypocotyls in dicots, and pulvini in monocots, respond by bending upward whereas roots typically bend downward. Gravitropism allows plants to readjust their growth to maximize light absorption for photosynthesis and to more efficiently acquire water and nutrients form the soil. Despite its significance for plant survival, there are still major gaps in understanding the cellular and molecular processes by which plants respond to gravity. The major aim of this proposal was to develop improved fluorescence labeling techniques to aid in understanding how the cytoskeleton modulated plant responses to gravity.

Blancaflor, Elison B.↗

Discovery of Activities via Statistical Clustering of Fixation Patterns

Human behavior often consists of a series of distinct activities, each characterized by a unique signature of visual behavior. This is true even in a restricted domain, such as piloting an aircraft, where patterns of visual signatures might represent activities like communicating, navigating, and monitoring. We propose a novel analysis method for gaze-tracking data, to perform blind discovery of these activities based on their behavioral signatures. The method is in some respects similar to recurrence analysis, but here we compare not individual fixations, but groups of fixations aggregated over a fixed time interval. The duration of this interval is a parameter that we will refer to as τ. We assume that the environment has been divided into a set of N different areas-of-interest (AOIs). For a given interval of time of duration τ, we compute the proportion of time spent fixating each AOI, resulting in an N-dimensional vector. These proportions can be converted to counts by multiplying by τ divided by the average fixation duration (another parameter that we fix at 280 milliseconds). We compare different intervals by computing the chi-square statistic. The p-value associated with the statistic is the likelihood of observing the data under the hypothesis that the data in the two intervals were generated by a single process with a single set of probabilities governing the fixation of each AOI. We have investigated the method using a set of 10 synthetic "activities," that sample 4 AOIs. Four of these activities visit 3 of the 4 AOIs, with equal probability; as there are four different ways to leave-one- out, there are four such activities. Similarly, there are six different activities that leave-two-out. Sequences of simulated behavior were generated by running each activity for 40 seconds, in sequence, for a total of 6.7 minutes. The figure to the right shows the matrix of chi-square statistics, using a value of 2.8 seconds for τ, corresponding to 10 fixations. Low values (dark) indicate poor evidence for activity differences, while high values (bright) indicate strong evidence. The dark squares along the main diagonal each correspond to the forty second intervals in which the activity was held constant; the 4x4 block at the lower left corresponds to the four leave-one-out activities, while the 6x6 block in the upper right corresponds to the leave-two-out activities. (The anti-diagonal pattern of white squares indicates those activity pairs that share no AOIs.) The chi-square values can be binarized by choosing a particular significance level; we are interested in grouping bins that represent the same activity, effectively accepting the null hypothesis. Therefore, we may adopt a relatively lax criterion; for example, choosing a p-value of 0.2 means that two behaviors that have only a 1-in-5 chance of being produced by a single activity might nevertheless be clustered together. We have explored several methods to perform clustering on the data and solving for the activity probabilities. Greedy methods begin by selecting the time bin that is similar to the most (or least) other bins, and then forming a cluster from it and all other non-discriminable bins. These methods show mediocre performance, as they do not take into account temporal contiguity. Preliminary results indicate that methods that "grow" clusters in time from seed points perform better.

activity analysis↗

Assessing Alignment Between a Top-Down LCA Model and a Bottom-Up Supply Chain Model for Data Gap Identification and Prioritization

This work presents a method for evaluating the alignment between the results of bottom-up and top-down models and using the evaluation to enhance the bottom-up model and underlying data set. Disaggregation is used to enhance the embodied energy results of top-down models, which are then compared to bottom-up model results. Using the disaggregated top-down results makes it possible to identify specific data gaps and deficiencies within the bottom-up model and its data set. The top-down results will also provide information around which data gaps in the bottom-up model are contributing the most error to the results; in this way, data gaps can be identified as either critical or non-critical, and the effort needed to acquire and add data can be prioritized. Although the proposed method could be applied to any physical quantity or environmental impact that can be quantified with both bottom-up models (typically in physical units) and top-down models (typically in monetary units), embodied energy is the focus of this work. The disaggregated energy baseline is calculated from the throughput vector x, which gives the total economic activity required from each sector to provide a final demand. Using the direct requirements matrix, each element in x is disaggregated into a vector of inputs to the corresponding sector. Energy data from the interventions matrix B is then used to convert the monetary flows of the disaggregated x into physical energy flows. For each energy flow, the origin sector, the destination sector, and the energy carrier type will all be known. These energetic flows are analogous but not identical to direct energy inputs that would appear in a unit process used in life cycle analysis and other bottom-up methods, and thus can be used to evaluate the alignment between the top-down results and the results of a bottom-up model. To demonstrate and further develop the proposed method, embodied energy for chemicals assessed in the 2015 U.S. DOE bandwidth study on energy use within the chemical sector will be calculated using a top-down and a bottom-up model. The Materials Flows through Industry (MFI) modeling tool developed at the National Renewable Energy Laboratory is used as the bottom-up model, and the U.S. EEIO model, recently published by the U.S. Environmental Protection Agency, is used as the top-down model. U.S. EEIO and MFI results will be evaluated to determine how well the model results align. Where the two models are not in good alignment, the disaggregation method described above will be applied to the top-down model to generate a more detailed set of results that will aid in identifying data gaps in the MFI database. Using only MFI, data gaps can be identified in terms of which sectors have few or no unit processes in the database, but no information is available regarding the impact of the missing data on MFI results. The disaggregated top-down results will provide this information on which gaps in the MFI data are critical and have a significant impact on the results, and which are less important.

29 ENERGY PLANNING, POLICY, AND ECONOMY↗

Predictive analytics of selections of russet potatoes

We explore the application of machine learning algorithms specifically to enhance the selection process of Russet potato (Solanum tuberosum L.) clones in breeding trials by predicting their suitability for advancement. This study addresses the challenge of efficiently identifying high-yield, disease-resistant, and climate-resilient potato varieties that meet processing industry standards. Leveraging manually collected data from trials in the state of Oregon, we investigate the potential of a wide variety of state-of-the-art binary classification models. The dataset includes 1086 clones, with data on 38 attributes recorded for each clone, focusing on yield, size, appearance, and frying characteristics, with several control varieties planted consistently across four Oregon regions from 2013 to 2021. We conduct a comprehensive analysis of the dataset that includes preprocessing, feature engineering, and imputation to address missing values. We focus on several key metrics such as accuracy, F1-score, and Matthews correlation coefficient (MCC) for model evaluation. The top-performing models, namely a feedforward neural network classifier (Neural Net), a histogram-based gradient boosting classifier (HGBC), and a support vector machine classifier (SVM), demonstrate consistent and significant results. To further validate our findings, we conducted a simulation study using the aims, data-generating mechanisms, estimands, methods, and performance measures (ADEMP) framework, simulating different data-generating scenarios to assess model robustness and performance through true positive, true negative, false positive, and false negative distributions, area under the receiver operating characteristic curve (AUC-ROC) and MCC. The simulation results highlight that non-linear models like SVM and HGBC consistently show higher AUC-ROC and MCC than logistic regression, thus outperforming the traditional linear model across various distributions, and emphasizing the importance of model selection and tuning in agricultural trials. Variable selection further enhances model performance and identifies influential features in predicting trial outcomes. The findings emphasize the potential of machine learning in streamlining the selection process for potato varieties, offering benefits such as increased efficiency, substantial cost savings, and judicious resource utilization. Our study contributes insights into precision agriculture and showcases the relevance of advanced technologies for informed decision-making in breeding programs.

60 APPLIED LIFE SCIENCES↗

State estimation Kalman filter using optical processings Noise statistics known

Reference is made to a study by Casasent et al. (1983), which gave a description of a frequency-multiplexed acoustooptic processor and showed how it was capable of performing all the individual operations required in Kalman filtering. The data flow and organization of all required operations however, were not detailed in that study. Consideration is given here to a simpler Kalman filter state estimation problem. Equally spaced time-sampled intervals (k times T sub s, with k the iterative time index) are assumed. It is further assumed that the system noise vector w and the measurement noise vector v are uncorrelated and Gaussian distributed and that the noise statistics (Q and R) and the system model (Phi, Gamma, H) are known. The error covariance matrix P and the extrapolated error covariance matrix M can thus be precomputed and the Kalman gain matrix K sub k can be precomputed and stored for each input time sample.

Jackson, J.↗

R-parity violating decays of Bino neutralino LSPs at the LHC

The R-parity violating decays of Bino neutralino LSPs are analyzed within the context of the B - L MSSM “heterotic standard model”. These LSPs correspond to statistically determined initial soft supersymmetry breaking parameters which, when evolved using the renormalization group equations, lead to an effective theory satisfying all phenomenological requirements; including the observed electroweak vector boson masses and the Higgs mass. The explicit RPV decay channels of these LSPs into standard model particles, the analytic and numerical decay rates and the associated branching ratios are presented. The analysis of these quantities breaks into two separate calculations; first, for Bino neutralino LSPs with mass larger than MW± and, second, when the Bino neutralino mass is smaller than the electroweak scale. The RPV decay processes in both of these regions is analyzed in detail. The decay lengths of these RPV interactions are discussed. It is shown that for heavy Bino neutralino LSPs the vast majority of these decays are “prompt”, although a small, but calculable, number correspond to “displaced” decays of various lengths. The situation is reversed for light Bino LSPs, only a small number of which can RPV decay promptly. The relation of these results to the neutrino hierarchy–either normal or inverted–is discussed in detail.

79 ASTRONOMY AND ASTROPHYSICS↗

$\mathrm{SIM}$-$\mathrm{STEM}$ $\mathrm{Lab}$: Incorporating Compressed Sensing Theory for Fast STEM Simulation

We report recently it has been shown that precise dose control and an increase in the overall acquisition speed of atomic resolution scanning transmission electron microscope (STEM) images can be achieved by acquiring only a small fraction of the pixels in the image experimentally and then reconstructing the full image using an inpainting algorithm. In this paper, we apply the same inpainting approach (a form of compressed sensing) to simulated, sub-sampled atomic resolution STEM images. We find that it is possible to significantly sub-sample the area that is simulated, the number of g-vectors contributing the image, and the number of frozen phonon configurations contributing to the final image while still producing an acceptable fit to a fully sampled simulation. Here we discuss the parameters that we use and how the resulting simulations can be quantifiably compared to the full simulations. As with any Compressed Sensing methodology, care must be taken to ensure that isolated events are not excluded from the process, but the observed increase in simulation speed provides significant opportunities for real time simulations, image classification and analytics to be performed as a supplement to experiments on a microscope to be developed in the future

47 OTHER INSTRUMENTATION↗

PETSc TSAdjoint: A Discrete Adjoint ODE Solver for First-Order and Second-Order Sensitivity Analysis

Here, we present a new software system PETSc TSAdjoint for first-order and second order adjoint sensitivity analysis of time-dependent nonlinear differential equations. The derivative calculation in PETSc TSAdjoint is essentially a high-level algorithmic differentiation process. The adjoint models are derived by differentiating the timestepping algorithms and implementing them based on the parallel infrastructure in PETSc. Full differentiation of the library code, including MPI routines, is avoided, and users do not need to derive their own adjoint models for their specific applications. PETSc TSAdjoint can compute the first-order derivative, that is, the gradient of a scalar functional, and the Hessian-vector product, which carries second-order derivative information, while requiring minimal input (a few callbacks) from the users. The adjoint model employs optimal checkpointing schemes in a manner that is transparent to users. Finally, usability, efficiency, and scalability are demonstrated through examples from a variety of applications.

79 ASTRONOMY AND ASTROPHYSICS↗

Radar studies in the solar system

We are actively engaged in observations of asteroids and comets, both as planned targets and as targets of opportunity. The most recent example of the latter is asteroid 1991 AQ, which was discovered optically on January 14, 1991 and found to be rapidly nearing Earth. Due in part to our previous success in obtaining useful radar data on short notice, we were able to schedule time on the Arecibo radar on January 28-31, when the asteroid was within the Arecibo declination window. Each day during the period, a fresh ephemeris was made including all available data, both optical and radar. With further processing, the results of the observations are expected to include detailed two-dimensional images of the asteroid and information on its surface properties, size, shape and spin. The observing program also included two other asteroids, two of the four Galilean satellites of Jupiter, the satellites of Mars, and the planet Mercury. Analysis of newly available radar observations of Venus has led to a refinement of the spin vector of that planet and has thereby provided a coordinate basis for the Magellan spacecraft mapping mission.

Shapiro, Irwin I.↗