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At least 145 records · Page 8

Culturability as an indicator of succession in microbial communities

Successional theory predicts that opportunistic species with high investment of energy in reproduction and wide niche width will be replaced by equilibrium species with relatively higher investment of energy in maintenance and narrower niche width as communities develop. Since the ability to rapidly grow into a detectable colony on nonselective agar medium could be considered as characteristic of opportunistic types of bacteria, the percentage of culturable cells may be an indicator of successional state in microbial communities. The ratios of culturable cells (colony forming units on R2A agar) to total cells (acridine orange direct microscopic counts) and culturable cells to active cells (reduction of 5-cyano-2,3-ditolyl tetrazolium chloride) were measured over time in two types of laboratory microcosms (the rhizosphere of hydroponically grown wheat and aerobic, continuously stirred tank reactors containing plant biomass) to determine the effectiveness of culturabilty as an index of successional state. The culturable cell:total cell ratio in the rhizosphere decreased from approximately 0.25 to less than 0.05 during the first 30-50 days of plant growth, and from 0.65 to 0.14 during the first 7 days of operation of the bioreactor. The culturable cell:active cell ratio followed similar trends, but the values were consistently greater than the culturable cell:total cell ratio, and even exceeded I in early samples. Follow-up studies used a cultivation-independent method, terminal restriction fragment length polymorphisms (TRFLP) from whole community DNA, to assess community structure. The number of TRFLP peaks increased with time, while the number of culturable types did not, indicating that the general decrease in culturability is associated with a shift in community structure. The ratio of respired to assimilated C-14-labeled amino acids increased with the age of rhizosphere communities, supporting the hypothesis that a shift in resource allocation from growth to maintenance occurs with time. Results from this work indicate that the percentage of culturable cells may be a useful method for assessing the successional state of microbial communities.

NASA Center KSC↗

A global change data base using Thematic Mapper data - Earth Monitoring Educational System (EMES)

Some of the main directions in creating an education program in earth system science aimed at combining top science and technology with high academic performance are presented. The creation of an Earth Monitoring Educational System (EMES) integrated with the research interests of the NASA Ames Research Center and one or more universities is proposed. Based on the integration of a global network of cooperators to build a global data base for assessments of global change, EMES would promote degrees at all levels in global ecology at associated universities and colleges, and extracurricular courses for multilevel audiences. EMES objectives are to: train specialists; establish a tradition of solving regional problems concerning global change in a systemic manner, using remote sensing technology as the monitoring tool; and transfer knowledge on global change to the national and world communities. South America is proposed as the pilot continent for the project.

D'Antoni, Hector L.↗

Continuous Nanoclimate Data (1985-1988) from the Ross Desert (McMurdo Dry Valleys) Cryptoendolithic Microbial Ecosystem

We have collected year-round nanoclimate data for the cryptoendolithic microbial habitat in sandstones of the Ross desert, Antarctica, obtained with an Argos satellite data system. Data for two sites in the McMurdo Dry Valleys are available: Linnaeus Terrace, January 1985 to June 1988, and Battleship Promontory, 1986-1987. The focus of this research is ecological, and hence year-round environmental data have been obtained for the ambient environment as well as for conditions within the rock. Using data from the summer, we compare the conditions inside the rock to the outside weather. This demonstrates how the rock provides a shelter for the endolithic microbial community. The most important property of the rock is that it absorbs the summer sunlight, thereby warming up to temperatures above freezing. This warming allows snowmelt to seep into the rock, and the moisture level in the rocks can remain high for weeks against loss to the dry environment.

McKay, Christopher P.↗

Multi Sensor Approach to Address Sustainable Development

The main objectives of Earth Science research are many folds: to understand how does this planet operates, can we model her operation and eventually develop the capability to predict such changes. However, the underlying goals of this work are to eventually serve the humanity in providing societal benefits. This requires continuous, and detailed observations from many sources in situ, airborne and space. By and large, the space observations are the way to comprehend the global phenomena across continental boundaries and provide credible boundary conditions for the mesoscale studies. This requires a multiple sensors, look angles and measurements over the same spot in accurately solving many problems that may be related to air quality, multi hazard disasters, public health, hydrology and more. Therefore, there are many ways to address these issues and develop joint implementation, data sharing and operating strategies for the benefit of the world community. This is because for large geographical areas or regions and a diverse population, some sound observations, scientific facts and analytical models must support the decision making. This is crucial for the sustainability of vital resources of the world and at the same time to protect the inhabitants, endangered species and the ecology. Needless to say, there is no single sensor, which can answer all such questions effectively. Due to multi sensor approach, it puts a tremendous burden on any single implementing entity in terms of information, knowledge, budget, technology readiness and computational power. And, more importantly, the health of planet Earth and its ability to sustain life is not governed by a single country, but in reality, is everyone's business on this planet. Therefore, with this notion, it is becoming an impractical problem by any single organization/country to bear this colossal responsibility. So far, each developed country within their means has proceeded along satisfactorily in implementing their Earth observing needs but it has left a big void in the developing world who have very limited resources to invest in the space measurements. This paper gives some serious thoughts in what options are there in undertaking this tremendous challenge. The problem is multi-dimensional in terms of budget, technology availability, environmental legislations, public awareness, and communication limitations. Some of these issues are introduced, discussed and possible implementation strategies are provided in this paper to move out of this predicament. A strong emphasis is placed on international cooperation and collaboration to see a collective benefit for this effort

Habib, Shahid↗

2018 NISAR Applications Workshop: Agriculture and Soil Moisture

Agricultural lands cover the globe and play an essential role in not only sustaining a growing global population, but can have significant implications on the Earth system through land use change (e.g., deforestation, grazing, etc.). As such, countries around the world have dedicated programs for managing these lands. Accurate and timely information concerning the status of agricultural crops (soil moisture, crop health, crop type, etc.) is essential to those nations’ anthropogenic and ecological health as well as economy. The joint NASA/US Department of Agriculture Agricultural Research Service (USDA-ARS) workshop focused on advancing agriculture and soil moisture applications by using remote sensing data from the NASA-ISRO Synthetic Aperture Radar (NISAR) mission (expected launch 2022). Participants included representatives from the international agriculture community that are key players in facilitating integration of Earth Observations into decision support workflows including US Federal Agencies, nonprofits, and private sector. They included scientists, technicians, and program managers with a responsibility for data acquisition and exploitation such as product development, delivery, and use, as well as capacity building. Discussions were held over two and a half days to convey the broader agriculture and soil moisture community information needs, the mission and procedures for various representative participants and programs involved in the delivery of geospatial products, and the capabilities and status of the NISAR mission. Case studies were presented to demonstrate the current state of practice in the use of SAR remote sensing for applications of direct importance for the agriculture and soil moisture communities. Eleven organizations presented their information requirements in response to a set of questions provided by the NASA team, then the NASA team responded by describing the degree to which NISAR could meet these requirements. Discussion ensued about needed data product specifications to increase utility (e.g., projection, latency, etc.), tools and capacity building.

Stavros, Natasha↗

Grand Valley Ecological Forecasting : Assessing Trends in Pinyon-Juniper Habitat Relative to Drought, Beetle Infestation, Wildland Fires, and Treatment to Plan Future Management Strategies

Drought, beetle infestation, and more frequent wildfires are changing the composition and distribution of the pinyon-juniper woodland and sagebrush ecosystems of the Grand Valley in western Colorado. Land managers must consider short- and long-term goals for restoration as well as budgetary and personnel limitations after such disturbances. Satellite remote sensing can provide long-term and continuous vegetation monitoring to assess where restoration is needed most and where treatment practices are most likely to succeed. Harnessing Earth observation data, our team set out to observe trends in disturbances and the distribution of pinyon-juniper woodlands and sagebrush communities of National Park Service (NPS) and Bureau of Land Management (BLM) lands within the Grand Valley. We used imagery from the Moderate Resolution Imaging Spectroradiometer (MODIS) aboard the Aqua and Terra satellites, and Landsat sensors to map landcover change within these ecosystems from 1984-2021. Additionally, we analyzed disturbed areas and treatment sites to understand their effect on long-term vegetation health and recovery. Results showed that pinyon-juniper woodland has expanded in extent more than other landcover types, indicating woody encroachment into sagebrush ecosystems. We also found that wildfire disturbances had lasting impacts up to 20 years post-disturbance. Pre-fire treatment practices showed mixed results regarding their effectiveness at stopping fires and promoting post-fire recovery. These results will provide context to public land managers in the Grand Valley when developing management plans, ecological monitoring locations, and implementing treatment practices for future disturbances.

Garrett Powers↗

Using emergent order to shape a space society

A fast-growing movement in the scientific community is reshaping the way that we view the world around us. The short-hand name for this movement is 'chaos'. Chaos is a science of the global, nonlinear nature of systems. The center of this set of ideas is that simple, deterministic systems can breed complexity. Systems as complex as the human body, ecology, the mind or a human society. While it is true that simple laws can breed complexity, the other side is that complex systems can breed order. It is the latter that I will focus on in this paper. In the past, nonlinear was nearly synonymous with unsolvable because no general analytic solutions exist. Mathematically, an essential difference exists between linear and nonlinear systems. For linear systems, you just break up the complicated system into many simple pieces and patch together the separated solutions for each piece to form a solution to the full problem. In contrast, solutions to a nonlinear system cannot be added to form a new solution. The system must be treated in its full complexity. While it is true that no general analytical approach exists for reducing a complex system such as a society, it can be modeled. The technical involves a mathematical construct called phase space. In this space stable structures can appear which I use as analogies for the stable structures that appear in a complex system such as an ecology, the mind or a society. The common denominator in all of these systems is that they rely on a process called feedback loops. Feedback loops link the microscopic (individual) parts to the macroscopic (global) parts. The key, then, in shaping a space society, is in effectively using feedback loops. This paper will illustrate how one can model a space society by using methods that chaoticists have developed over the last hundred years. And I will show that common threads exist in the modeling of biological, economical, philosophical, and sociological systems.

Graps, Amara L.↗

Flora: A Proposed Hyperspectral Mission

In early 2004, one of the authors (Stephen Ungar, NASA GSFC) presented a mission concept called "Spectrasat" at the AVIRIS Workshop in Pasadena, CA. This mission concept grew out of the lessons learned from the Earth Observing-One (EO-1) Hyperion Imaging Spectrometer and was structured to more effectively accomplish the types of studies conducted with Hyperion. The Spectrasat concept represented an evolution of the technologies and operation strategies employed on EO-I. The Spectrasat concept had been preceded by two community-based missions proposed by Susan Ustin, UC Davis and Robert Green, NASA JPL. As a result of community participation, starting at this AVIRIS Workshop, the Spectrasat proposal evolved into the Flora concept which now represents the combined visions of Gregory Asner (Carnegie Institute), Stephen Ungar, Robert Green and Robert Knox, NASA GSFC. Flora is a proposed imaging spectrometer mission, designed to address global carbon cycle science issues. This mission centers on measuring ecological disturbance for purposes of ascertaining changes in global carbon stocks and draws heavily on experience gained through AVIRIS airborne flights and Hyperion space born flights. The observing strategy exploits the improved ability of imaging spectrometers, as compared with multi-spectral observing systems, to identify vegetation functional groups, detect ecosystem response to disturbance and assess the related discovery. Flora will be placed in a sun synchronous orbit, with a 45 meter pixel size, a 90 km swath width and a 31 day repeat cycle. It covers the spectral range from 0.4 to 2.5 micrometers with a spectral sampling interval of 10 nm. These specifications meet the needs of the Flora science team under the leadership of Gregory Asner. Robert Green, has introduced a spectrometer design for Flora which is expected to have a SNR of 600: 1 in the VNIR and 450: 1 in the SWIR. The mission team at NASA GSFC is designing an Intelligent Payload Module (IPM) designed to effectively reduce the volume of data required to be transmitted down to the ground. This paper discusses mission science objectives, describes the mission concept and presents the current status of possible funding opportunities leading to realization of the mission.

Ungar, Stephen↗

Wetlands ecology

The author has identified the following significant results. The ERTS imagery analyzed provides approximately 2/3 coverage of the test site. Analysis was made using visual methods, density slicing, and multispectral analysis. Preliminary conclusions reached are that most, if not all, of the investigation objectives can be met. Saline and near-saline wetlands can be delineated from ERTS-1 images as the wetland-upland boundaries and land-water interface are clearly defined. Major plant species or communities such as Spartina alterniflora (high and low vigor forms), Spartina patens/Distichlis spicata, and Juncus roemarianus can be discriminated and spoil disposal areas identified.

Anderson, R. R.↗

EOS Terra Validation Program

The EOS Terra mission will be launched in July 1999. This mission has great relevance to the atmospheric radiation community and global change issues. Terra instruments include Advanced Spaceborne Thermal Emission and Reflection Radiometer (ASTER), Clouds and Earth's Radiant Energy System (CERES), Multi-Angle Imaging Spectroradiometer (MISR), Moderate Resolution Imaging Spectroradiometer (MODIS) and Measurements of Pollution in the Troposphere (MOPITT). In addition to the fundamental radiance data sets, numerous global science data products will be generated, including various Earth radiation budget, cloud and aerosol parameters, as well as land surface, terrestrial ecology, ocean color, and atmospheric chemistry parameters. Significant investments have been made in on-board calibration to ensure the quality of the radiance observations. A key component of the Terra mission is the validation of the science data products. This is essential for a mission focused on global change issues and the underlying processes. The Terra algorithms have been subject to extensive pre-launch testing with field data whenever possible. Intensive efforts will be made to validate the Terra data products after launch. These include validation of instrument calibration (vicarious calibration) experiments, instrument and cross-platform comparisons, routine collection of high quality correlative data from ground-based networks, such as AERONET, and intensive sites, such as the SGP ARM site, as well as a variety field experiments, cruises, etc. Airborne simulator instruments have been developed for the field experiment and underflight activities including the MODIS Airborne Simulator (MAS) AirMISR, MASTER (MODIS-ASTER), and MOPITT-A. All are integrated on the NASA ER-2 though low altitude platforms are more typically used for MASTER. MATR is an additional sensor used for MOPITT algorithm development and validation. The intensive validation activities planned for the first year of the Terra mission will be described with emphasis on derived geophysical parameters of most relevance to the atmospheric radiation community.

Starr, David↗

Wildfire Segmentation From Remotely Sensed Data Using Quantum-Compatible Conditional Vector Quantized-Variational Autoencoders

Wildfires represent a critical environmental hazard with multifaceted implications for ecosystems, communities, and public health [1]. The escalating frequency and intensity of wildfires globally have intensified the urgency for robust segmentation methodologies to facilitate effective mitigation, response, and recovery strategies [2]. Accurate wildfire segmentation is pivotal for delineating fire boundaries, assessing progression patterns, and prioritizing resource allocation during emergency scenarios. Furthermore, precise segmentation enables stakeholders, including policymakers, environmental scientists, and emergency responders, to formulate evidence-based strategies, thereby minimizing socio-economic disruptions and ecological degradation. Consequently, advancing wildfire segmentation techniques through innovative technological interventions remains a paramount research imperative. Although foundational in wildfire segmentation, traditional deterministic models exhibit inherent limitations that compromise their efficacy in dynamic and uncertain environments. These models often operate on rigid algorithms prioritizing deterministic classifications, thereby overlooking the inherent complexities and uncertainties associated with wildfire behavior and satellite data variability. Such deterministic frameworks tend to produce oversimplified representations that fail to capture the intricate nuances of evolving fire dynamics, spatial heterogeneity, and environmental interactions [1]. Consequently, the deterministic approach’s propensity for uncertainty collapsing [1, 3] hampers the accuracy, reliability, and applicability of segmentation outcomes in real-world scenarios. Contrastingly, stochastic models offer a more nuanced and adaptable framework for wildfire segmentation. By integrating probabilistic elements into the modeling paradigm, stochastic approaches, particularly probabilistic approaches such as variational auto encoders (VAEs) [4], facilitate comprehensive uncertainty assessment, enabling researchers to quantify and incorporate uncertainties into segmentation outcomes effectively. This probabilistic nature empowers stochastic models to encapsulate variability, account for data inconsistencies, and adapt to evolving environmental conditions, enhancing segmentation accuracy, reliability, and robustness. Embracing stochastic methodologies thus catalyzes advancements in wildfire science by fostering a more holistic, adaptive, and resilient segmentation framework. Despite VAEs demonstrating significant promise in various applications, they come with inherent limitations that have garnered attention within the machine learning community. One of the primary drawbacks lies in their reliance on static priors, which essentially assume a fixed distribution for latent variables, thereby limiting the model’s flexibility to capture complex data structures effectively [5]. This static nature leads to suboptimal representations, especially when dealing with complex and high-dimensional data. Additionally, VAEs often struggle with generating sharp and realistic samples, a phenomenon commonly referred to as mode collapse [5, 7, 6]. Furthermore, the optimization process in VAEs, which involves balancing the reconstruction loss and the regularization term, can sometimes be challenging to fine-tune [7]. In recent efforts to address these shortcomings, alternative approaches like Vector Quantized Variational Auto encoders(VQ-VAEs) [7], address the challenges by incorporating discrete latent variables and leveraging techniques that enhance the quality and diversity of generated samples while maintaining efficient training dynamics. VQ-VAEs propose a dynamic prior distribution generation mechanism that diverges from the static priors commonly associated with traditional VAEs. This dynamic approach allows for more adaptive and context-aware latent variable representations, thereby potentially capturing complex data structures more effectively. Unlike autoregressive prior models such as PixelCNN, which, despite their ability to model dependencies across data dimensions, suffer from significant computational inefficiencies and lack flexibility in handling diverse datasets. In our work, we propose to use a generative quantum-compatible approach to help alleviate the shortcomings of autoregressive prior model in VQ-VAEs. Restricted Boltzmann Machines (RBMs) are a viable alternative prior model that can learn prior distributions in a faster and more flexible manner. In this research endeavor, we meticulously curate a state-of-the-art dataset leveraging satellite MODIS data in conjunction with VIIRS fire masks, derived from Fire Radiative Power (FRP), thereby encapsulating diverse wildfire scenarios and environmental contexts. We developed a conditional VQ-VAE architecture with the RBM prior model that is trained in a supervised manner for segmenting wildfire masks. This innovative approach synergistically harnesses deep learning capabilities, enabling the generation of segmentation maps characterized by heightened precision, granularity, and contextual relevance. Furthermore, replacing the autoregressive prior learning method proposed by the original VQ-VAE with a prior density approximation via quantum-compatible RBM facilitates expedited inference processes, augments flexibility in prior sampling, optimizes computational efficiency and establishes a groundbreaking benchmark in wildfire segmentation methodologies.

quantum machine learning↗

Paleoenvironmental distribution of microfossils and stromatolites in the Upper Proterozoic Backlundtoppen Formation, Spitsbergen

The Upper Proterozoic (ca. 700-800 Ma old) Backlundtoppen Formation, northeastern Spitsbergen, preserves an abundant and varied record of ancient microbial life. Five distinctive microfossil assemblages occur in five equally distinct sedimentary settings; differences among the assemblages appear to reflect original ecological heterogeneity, although taphonomic circumstance may contribute to some distinctions. Microfossil assemblages occur in: oncolites, oolites, and pisolites; stratiform stromatolites and associated intraclastic rudites; partially silicified micrites; and siltites interbedded with quartz arenites. Individual assemblages contain one to eight differentiable taxa; a minimum of 17 distinct populations is present in the formation as a whole. Additional microbial community diversity an be inferred from the presence of domal, columnar, pseudocolumnar, and coniform stromatolites, none of which contains microfossils. On the basis of macrostructure, four stromatolite types appear to be present, but only three distinct mat-building communities can be inferred from microstructural features. Eohyella elongata n. sp., a euendolithic cyanobacterium found in silicified pisolites, is described as new.

Non-NASA Center↗

Migration Theory in Climate Mobility Research

The purpose of this article is to explore how migration theory is invoked in empirical studies of climate-related migration, and to provide suggestions for engagement with theory in the emerging field of climate mobility. Theory is critical for understanding processes we observe in social-ecological systems because it points to a specific locus of attention for research, shapes research questions, guides quantitative model development, influences what researchers find, and ultimately informs policies and programs. Research into climate mobility has grown out of early studies on environmental migration, and has often developed in isolation from broader theoretical developments in the migration research community. As such, there is a risk that the work may be inadequately informed by the rich corpus of theory that has contributed to our understanding of who migrates; why they migrate; the types of mobility they employ; what sustains migration streams; and why they choose certain destinations over others. On the other hand, there are ways in which climate and broader environment migration research is enriching the conceptual frameworks being employed to understand migration, particularly forced migration. This paper draws on a review of 75 empirical studies and modeling efforts conducted by researchers from a diversity of disciplines, covering various regions, and using a variety of data sources and methods to assess how they used theory in their research. The goal is to suggest ways forward for engagement with migration theory in this large and growing research domain.

migration theory↗

The ecological variations in thermal infrared emissivity of vegetation

The author has identified the following significant results. Through a series of contrasts, the statistical significance of differences in emissivity was determined for vegegation in dry and humid deserts, montane and deciduous rain forests, and the temperate region. No significant differences were found between the two types of desert vegetation or among the types of nondesert vegetation. However, the rain forest vegetation was significantly different from that of the temperate region. On a community-wide level, there is some physiological adaptation in plants to their radiational environment.

Arp, G. K.↗

Utilization of the water soluable fraction of wheat straw as a plant nutrient source

Recovery of water soluble, inorganic nutrients from the inedible portion of wheat was found to be an effective means of recycling nutrients within hydroponic systems. Through aqueous extraction (leaching), 60 percent of the total inorganic nutrient weight was removed from wheat straw and roots, although the recovery of individual nutrients varied. Leaching also removed about 20 percent of the total organic carbon from the biomass. In terms of dry weight, the leachate was comprised of approximately 60 percent organic and 40 percent inorganic compounds. Direct use of wheat straw leachate in static hydroponic systems had an inhibitory effect on wheat growth, both in the presence and absence of microorganisms. Biological treatment of leachate either with a mixed microbial community or the oyster mushroom Pleurotus ostreatus L., prior to use in hydroponic solutions, significantly reduced both the organic content and the inhibitory effects of the leachate. The inhibitory effects of unprocessed leachate appear to be a result of rapidly acting phytotoxic compounds that are detoxified by microbial activity. Leaching holds considerable promise as a method for nutrient recycling in a Controlled Ecological Life Support System (CELSS).

Mackowiak, C. L.↗

An Operational Overview of the EXport Processes in the Ocean from RemoTe Sensing (EXPORTS) Northeast Pacific Field Deployment

The goal of the EXport Processes in the Ocean from RemoTe Sensing (EXPORTS) field campaign is to develop a predictive understanding of the export, fate, and carbon cycle impacts of global ocean net primary production. To accomplish this goal, observations of export flux pathways, plankton community composition, food web processes, and optical, physical, and biogeochemical (BGC) properties are needed over a range of ecosystem states. Here we introduce the first EXPORTS field deployment to Ocean Station Papa in the Northeast Pacific Ocean during summer of 2018, providing context for other papers in this special collection. The experiment was conducted with two ships: a Process Ship, focused on ecological rates, BGC fluxes, temporal changes in food web, and BGC and optical properties, that followed an instrumented Lagrangian float; and a Survey Ship that sampled BGC and optical properties in spatial patterns around the Process Ship. An array of autonomous underwater assets provided measurements over a range of spatial and temporal scales, and partnering programs and remote sensing observations provided additional observational context. The oceanographic setting was typical of late-summer conditions at Ocean Station Papa: a shallow mixed layer, strong vertical and weak horizontal gradients in hydrographic properties, sluggish sub-inertial currents, elevated macronutrient concentrations and low phytoplankton abundances. Although nutrient concentrations were consistent with previous observations, mixed layer chlorophyll was lower than typically observed, resulting in a deeper euphotic zone. Analyses of surface layer temperature and salinity found three distinct surface water types, allowing for diagnosis of whether observed changes were spatial or temporal.The 2018 EXPORTS field deployment is among the most comprehensive biological pump studies ever conducted. A second deployment to the North Atlantic Ocean occurred in spring 2021, which will be followed by focused work on data synthesis and modeling using the entire EXPORTS data set.

Biological pump↗

Temperature response of Antarctic cryptoendolithic photosynthetic microorganisms

Growth responses to temperatures between 12.5 [degrees] C and 25 degrees C were determined for five photosynthetic microorganisms isolated from the Ross Desert cryptoendolithic community. Among eukaryotic algae, two strains of Trebouxia sp. have an upper temperature limit of 20 degrees C, and two strains of Hemichloris antarctica of 25 degrees C. The cyanobacterium Chroococcidiopsis sp., in contrast, grows at temperatures above 25 degrees C. These and earlier studies suggest that the eukaryotic algae of the Antarctic cryptoendolithic community have an upper temperature limit near 25 degrees C.

NASA Discipline Exobiology↗

Microbial colonization of a closed growth chamber during hydroponic cultivation of lettuce

The goal of this study was to characterize sessile and planktonic microbiota that developed during two successive hydroponic cultures of lettuce in a closed chamber system. Coupons of polyvinyl chloride (PVC) placed in the nutrient solution lines were removed periodically, as were samples of the nutrient solutions and condensate from the air-handling system. The bacteria and fungi present on the coupons and in fluid samples were enumerated by direct plate counts. Disinfecting the hydroponic system with 0.1% hypochlorite and 0.1 N nitric acid reduced the bacterial densities in biofilm samples from 1 x 10 7 CFU/10 cm 2 to 1 x 10 1 CFU/10 cm 2 and eliminated culturable fungi; Staphylococcus sp., Pseudomonas sp., and Micrococcus sp. survived this procedure. Bacterial and fungal concentrations in all samples returned to predisinfection levels after 2 days of plant growth. Pseudomonas and Acremonium predominated both before and after disinfection. Fungal concentrations never exceeded 7 x 10 2 CFU/10 cm 2 . The coupon microbiota differed from that of the rhizoplane at harvest. Overall, the greatest numbers of species were found on the rhizoplane samples collected during the second crop. The microbial community changed little during individual crops or between successive crops. Diversity indices remained relatively constant for all samples.

Bacteria growth & development↗