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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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All sourcesNASA NTRSDOE OSTI
NASA NTRS1986

High-resolution imaging studies of the near-nucleus regions of comets

High-resolution, calibrated images of the near-nucleus regions of Halley, Giacobini-Zinner (for ICE encounter) and additional comets displaying widely differing levels of activity were acquired with a CCD sensor at the Cassegrain focus of the 3.6 m Canada-France-Hawaii Telescope (CFHT). The International Halley Watch designated interference filters were used. Successful correlation of ICE magnetic field data with the optical images obtained with the CFHT on the night of encounter (11 Sept. 1985) primarily through the H2O(+) and neighboring continuum passbands and with 300 km spatial resolution provokes reinterpretation of the observed structure of cometary ion tails. The observations of Halley, limited by bad weather, were made at significant periods of the apparition: the first, in December 1984 with the comet at 5.3 AU during the period of the onset of activity; and the last, in November 1985 with the comet near opposition. The December observations show evidence of residual activity. The 18 November data show a highly-condensed inner coma with an extreme falloff in brightness from the region of the nucleus. These data provide the basis for characterizing the gas and dust environment in the immediate vicinity of the active nucleus.

Goldberg, B. A.↗
DOE OSTI2013

Light-sheet Bayesian microscopy enables deep-cell super-resolution imaging of heterochromatin in live human embryonic stem cells

Heterochromatin in the nucleus of human embryonic cells plays an important role in the epigenetic regulation of gene expression. The architecture of heterochromatin and its dynamic organization remain elusive because of the lack of fast and high-resolution deep-cell imaging tools. We enable this task by advancing instrumental and algorithmic implementation of the localization-based super-resolution technique. We present light-sheet Bayesian super-resolution microscopy (LSBM). We adapt lightsheet illumination for super-resolution imaging by using a novel prism-coupled condenser design to illuminate a thin slice of the nucleus with high signal-to-noise ratio. Coupled with a Bayesian algorithm that resolves overlapping fluorophores from high-density areas, we show, for the first time, nanoscopic features of the heterochromatin structure in both fixed and live human embryonic stem cells. The enhanced temporal resolution allows capturing the dynamic change of heterochromatin with a lateral resolution of 50–60 nm on a time scale of 2.3 s. Light-sheet Bayesian microscopy opens up broad new possibilities of probing nanometer-scale nuclear structures and real-time sub-cellular processes and other previously difficult-to-access intracellular regions of living cells at the single-molecule, and single cell level.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2013

FIRST HIGH-RESOLUTION IMAGES OF THE SUN IN THE 2796 Å Mg II k LINE

We present the first high-resolution solar images in the Mg II k 2796 Å line. The images, taken through a 4.8 Å broad interference filter, were obtained during the second science flight of Sunrise in 2013 June by the Sunrise Filter Imager (SuFI) instrument. The Mg II k images display structures that look qualitatively very similar to images taken in the core of Ca II H. The Mg II images exhibit reversed granulation (or shock waves) in the internetwork regions of the quiet Sun, at intensity contrasts that are similar to those found in Ca II H. Very prominent in Mg II are bright points, both in the quiet Sun and in plage regions, particularly near the disk center. These are much brighter than at other wavelengths sampled at similar resolution. Furthermore, Mg II k images also show fibril structures associated with plage regions. Again, the fibrils are similar to those seen in Ca II H images, but tend to be more pronounced, particularly in weak plage.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2018

Kelvin probe force microscopy for high-resolution imaging of hydrogen in steel alloys [Poster]

Kelvin probe force microscopy (KPFM) was used to image and co-locate the presence of hydrogen in stainless steel (SS) with microstructure. Various SS samples were investigated, including forged, welded, LENS® fabricated, and pinch welded materials. KPFM images the local work function of a surface as a function of spatial position using atomic force microscopy. KPFM is capable of delivering high spatial (~10 nm) and potential (~5mV) resolution maps characterizing the microstrucure and the locally varying work function of surfaces. Hydrogen segregated at the surface of SS increases the local work function by either reducing tensile strain or by reducing the chemical potential of surface species. In a process known as hydrogen embrittlement, hydrogen segregates to and becomes trapped at extended defects (e.g. dislocations, boundaries, pores) which leads to the initiation of cracking and can result in structural failure. Hydrogen storage necessitates a proper understanding of the mechanisms of hydrogen embrittlement in SS. Developing advanced characterization techniques for hydrogen embrittlement is critical to identify long term solutions for materials exposed to hydrogen. Current techniques of observing hydrogen embrittlement involve mechanical testing, and optical microscopy with low resolution. Advance microstructural imaging techniques capable of resolving features down to the nanometer scale are needed. KPFM provides high resolution imaging for the characterization and development of SS components for hydrogen storage.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗
DOE OSTI2021

TEMImageNet training library and AtomSegNet deep-learning models for high-precision atom segmentation, localization, denoising, and deblurring of atomic-resolution images

Abstract Atom segmentation and localization, noise reduction and deblurring of atomic-resolution scanning transmission electron microscopy (STEM) images with high precision and robustness is a challenging task. Although several conventional algorithms, such has thresholding, edge detection and clustering, can achieve reasonable performance in some predefined sceneries, they tend to fail when interferences from the background are strong and unpredictable. Particularly, for atomic-resolution STEM images, so far there is no well-established algorithm that is robust enough to segment or detect all atomic columns when there is large thickness variation in a recorded image. Herein, we report the development of a training library and a deep learning method that can perform robust and precise atom segmentation, localization, denoising, and super-resolution processing of experimental images. Despite using simulated images as training datasets, the deep-learning model can self-adapt to experimental STEM images and shows outstanding performance in atom detection and localization in challenging contrast conditions and the precision consistently outperforms the state-of-the-art two-dimensional Gaussian fit method. Taking a step further, we have deployed our deep-learning models to a desktop app with a graphical user interface and the app is free and open-source. We have also built a TEM ImageNet project website for easy browsing and downloading of the training data.

25 ENERGY STORAGE↗
DOE OSTI2015

A search for fast radio bursts at low frequencies with Murchison Widefield Array high time resolution imaging

We present the results of a pilot study search for fast radio bursts (FRBs) using the Murchison Widefield Array (MWA) at low frequencies (139–170 MHz). We utilized MWA data obtained in a routine imaging mode from observations where the primary target was a field being studied for Epoch of Reionization detection. We formed images with 2 s time resolution and 1.28 MHz frequency resolution for 10.5 hr of observations, over 400 square degrees of the sky. We de-dispersed the dynamic spectrum in each of 372,100 resolution elements of 2 × 2 arcmin{sup 2}, between dispersion measures of 170 and 675 pc cm{sup −3}. Based on the event rate calculations in Trott et al., which assume a standard candle luminosity of 8 × 10{sup 37} Js{sup −1}, we predict that with this choice of observational parameters, the MWA should detect (∼10, ∼2, ∼0) FRBs with spectral indices corresponding to (−2, −1, 0), based on a 7σ detection threshold. We find no FRB candidates above this threshold from our search, placing an event rate limit of <700 above 700 Jy ms per day per sky and providing evidence against spectral indices α<−1.2 (S∝ν{sup α}). We compare our event rate and spectral index limits with others from the literature. We briefly discuss these limits in light of recent suggestions that supergiant pulses from young neutron stars could explain FRBs. We find that such supergiant pulses would have to have much flatter spectra between 150 and 1400 MHz than have been observed from Crab giant pulses to be consistent with the FRB spectral index limit we derive.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2014

Dynamics of femtosecond laser-induced periodic surface structures on silicon by high spatial and temporal resolution imaging

The formation dynamics of periodic ripples induced by femtosecond laser pulses (pulse duration τ = 50 fs and central wavelength λ = 800 nm) are studied by a collinear pump-probe imaging technique with a temporal resolution of 1 ps and a spatial resolution of 440 nm. The ripples with periods close to the laser wavelength begin to appear upon irradiation of two pump pulses at surface defects produced by the prior one. The rudiments of periodic ripples emerge in the initial tens of picoseconds after fs laser irradiation, and the ripple positions keep unmoved until the formation processes complete mainly in a temporal span of 1500 ps. The results suggest that the periodic deposition of laser energy during the interaction between femtosecond laser pulses and sample surface plays a dominant role in the formation of periodic ripples.

75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND↗
NASA NTRS1975

Silicon solid/state linear arrays for multispectral high resolution imaging systems

Solid-state, electronically scanned, linear detector arrays are now available which can be used in a pushbroom scan mode imaging system for high-resolution multispectral earth resource survey applications. These arrays provide high performance in the visible to near-IR region. Two performance criteria must be assessed in the choice of a detector array: signal-to-noise ratio and spectral response. Consideration of diffraction limitations shows that optics size cannot be significantly reduced by using very low-noise detectors. The required spectral response for a viable detector array should not have spectral ripples that cause a low-level, but significant, error in the detected effective reflectance of the target.

Thompson, L. L.↗
DOE OSTI2015

3D high spectral and spatial resolution imaging of ex vivo mouse brain

Purpose: Widely used MRI methods show brain morphology both in vivo and ex vivo at very high resolution. Many of these methods (e.g., T{sub 2}{sup *}-weighted imaging, phase-sensitive imaging, or susceptibility-weighted imaging) are sensitive to local magnetic susceptibility gradients produced by subtle variations in tissue composition. However, the spectral resolution of commonly used methods is limited to maintain reasonable run-time combined with very high spatial resolution. Here, the authors report on data acquisition at increased spectral resolution, with 3-dimensional high spectral and spatial resolution MRI, in order to analyze subtle variations in water proton resonance frequency and lineshape that reflect local anatomy. The resulting information compliments previous studies based on T{sub 2}{sup *} and resonance frequency. Methods: The proton free induction decay was sampled at high resolution and Fourier transformed to produce a high-resolution water spectrum for each image voxel in a 3D volume. Data were acquired using a multigradient echo pulse sequence (i.e., echo-planar spectroscopic imaging) with a spatial resolution of 50 × 50 × 70 μm{sup 3} and spectral resolution of 3.5 Hz. Data were analyzed in the spectral domain, and images were produced from the various Fourier components of the water resonance. This allowed precise measurement of local variations in water resonance frequency and lineshape, at the expense of significantly increased run time (16–24 h). Results: High contrast T{sub 2}{sup *}-weighted images were produced from the peak of the water resonance (peak height image), revealing a high degree of anatomical detail, specifically in the hippocampus and cerebellum. In images produced from Fourier components of the water resonance at −7.0 Hz from the peak, the contrast between deep white matter tracts and the surrounding tissue is the reverse of the contrast in water peak height images. This indicates the presence of a shoulder in the water resonance that is not present at +7.0 Hz and may be specific to white matter anatomy. Moreover, a frequency shift of 6.76 ± 0.55 Hz was measured between the molecular and granular layers of the cerebellum. This shift is demonstrated in corresponding spectra; water peaks from voxels in the molecular and granular layers are consistently 2 bins apart (7.0 Hz, as dictated by the spectral resolution) from one another. Conclusions: High spectral and spatial resolution MR imaging has the potential to accurately measure the changes in the water resonance in small voxels. This information can guide optimization and interpretation of more commonly used, more rapid imaging methods that depend on image contrast produced by local susceptibility gradients. In addition, with improved sampling methods, high spectral and spatial resolution data could be acquired in reasonable run times, and used for in vivo scans to increase sensitivity to variations in local susceptibility.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2023

Super-Resolution Imaging of Plant Receptor-Like Kinases Uncovers Their Colocalization and Coordination with Nanometer Resolution

Plant cell signaling often relies on the cellular organization of receptor-like kinases (RLKs) within membrane nanodomains to enhance signaling specificity and efficiency. Thus, nanometer-scale quantitative analysis of spatial organizations of RLKs could provide new understanding of mechanisms underlying plant responses to environmental stress. Here, we used stochastic optical reconstruction fluorescence microscopy (STORM) to quantify the colocalization of the flagellin-sensitive-2 (FLS2) receptor and the nanodomain marker, remorin, within Arabidopsis thaliana root hair cells. We found that recovery of FLS2 and remorin in the plasma membrane, following ligand-induced internalization by bacterial-flagellin-peptide (flg22), reached ~85% of their original membrane density after ~90 min. The pairs colocalized at the membrane at greater frequencies, compared with simulated randomly distributed pairs, except for directly after recovery, suggesting initial uncoordinated recovery followed by remorin and FLS2 pairing in the membrane. The purinergic receptor, P2K1, colocalized with remorin at similar frequencies as FLS2, while FLS2 and P2K1 colocalization occurred at significantly lower frequencies, suggesting that these RLKs mostly occupy distinct nanodomains. The chitin elicitor receptor, CERK1, colocalized with FLS2 and remorin at much lower frequencies, suggesting little coordination between CERK1 and FLS2. These findings emphasize STORM’s capacity to observe distinct nanodomains and degrees of coordination between plant cell receptors, and their respective immune pathways.

59 BASIC BIOLOGICAL SCIENCES↗
NASA NTRS1992

High-resolution imaging of QSO 2305 + 187 (= 4C 18.68)

Optical imaging of QSO 2305 + 187 with resolution ranging from 0.1 to 1.0 arcsec in continuum and line emission is presented. The data are from FOCAM and the HR Cam of the Canada-France-Hawaii Telescope, and the Wide Field Camera of the Hubble Space Telescope. These are compared with previously published optical and radio images. The QSO host galaxy has the basic properties of an elliptical, but has significant irregularities, and tidal tails which indicate an ongoing gravitational interaction with a close companion.

Hutchings, J. B.↗
DOE OSTI2015

High-resolution imaging of NGC 2346 with GSAOI/GeMS: Disentangling the planetary nebula molecular structure to understand its origin and evolution

We present high spatial resolution (≈60–90 mas) images of the molecular hydrogen emission in the planetary nebula (PN) NGC 2346. The data were acquired during the system verification of the Gemini Multi-conjugate Adaptive Optics System + Gemini South Adaptive Optics Imager. At the distance of NGC 2346, 700 pc, the physical resolution corresponds to ≈56 AU, which is slightly higher than an [N ii] image of NGC 2346 that was obtained with the Hubble Space Telescope/WFPC2. With this unprecedented resolution, for the first time we were able to study the structure of the H{sub 2} gas within the nebula in detail. We found it to be composed of knots and filaments, which at a lower resolution had appeared to be a uniform torus of material. We explain how the formation of the clumps and filaments in this PN are consistent with a mechanism in which a central hot bubble of nebular gas surrounding the central star has been depressurized, and the thermal pressure of the photoionized region drives the fragmentation of the swept-up shell.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2021

Super-resolution imaging illuminates new dynamic behaviors of cellulose synthase

Abstract Confocal imaging has shown that CELLULOSE SYNTHASE (CESA) particles move through the plasma membrane as they synthesize cellulose. However, the resolution limit of confocal microscopy circumscribes what can be discovered about these tiny biosynthetic machines. Here, we applied Structured Illumination Microscopy (SIM), which improves resolution two-fold over confocal or widefield imaging, to explore the dynamic behaviors of CESA particles in living plant cells. SIM imaging reveals that Arabidopsis thaliana CESA particles are more than twice as dense in the plasma membrane as previously estimated, helping explain the dense arrangement of cellulose observed in new wall layers. CESA particles tracked by SIM display minimal variation in velocity, suggesting coordinated control of CESA catalytic activity within single complexes and that CESA complexes might move steadily in tandem to generate larger cellulose fibrils or bundles. SIM data also reveal that CESA particles vary in their overlaps with microtubule tracks and can complete U-turns without changing speed. CESA track patterns can vary widely between neighboring cells of similar shape, implying that cellulose patterning is not the sole determinant of cellular growth anisotropy. Together, these findings highlight SIM as a powerful tool to advance CESA imaging beyond the resolution limit of conventional light microscopy.

59 BASIC BIOLOGICAL SCIENCES↗
NASA NTRS1986

Preliminary system concepts for MODIS - A moderate resolution imaging spectrometer for EOS

Plans are underway at NASA Goddard to develop a moderate-resolution (0.5-1.0-km) imaging spectrometer with 104 spectral bands in the range from 0.4 to 14.2 microns for use as a NASA facility aboard the Space Station Earth Observation System. Science requirements call for the system to image the earth's surface every 2 d for a period of 10 yr, using two electrooptic sensors. MODIS-T (tilt) has 64 10-nm-wide channels in the 400-1000-nm range. The system has 5-cm-diameter optics, scans + or - 45 deg, and can be pointed + or - 60 deg along track. Calculated S/N is in excess of 800:1 near 400 nm. MODIS-N (nadir) scans + or - 45 deg about nadir with 12 500-m- and 28 1000-m-resolution channels, including two polarization channels and 14 thermal-IR channels. With 20-cm-diameter optics the calculated S/N of the majority of the reflected solar bands is over 1000:1. NETD values for the thermal bands are on the order of 0.1-0.2 K at 300 K. Combined MODIS-T and MODIS-N data rates are 8.3 Mb/s in daylight and 1.5 Mb/s at night, resulting in a total of 360 Gb/d.

Barnes, W. L.↗
NASA NTRS1986

Interpreting forest and grassland biome productivity utilizing nested scales of image resolution and biogeographical analysis

Data acquisition, initial site characterization, image and geographic information methods available, and brief evaluations of first-year for NASA's Thematic Mapper (TM) working group are presented. The TM and other spectral data are examined in order to relate local, intensive ecosystem research findings to estimates of carbon cycling rates over wide geographic regions. The effort is to span environments ranging from dry to moist climates and from good to poor site quality using the TM capability, with and without the inclusion of geographic information system (GIS) data, and thus to interpret the local spatial pattern of factors conditioning biomass or productivity. Twenty-eight TM data sets were acquired, archived, and evaluated. The ERDAS image processing and GIS system were installed on the microcomputer (PC-AT) and its capabilities are being investigated. The TM coverage of seven study areas were exported via ELAS software on the Prime to the ERDAS system. Statistical analysis procedures to be used on the spectral data are being identified.

Iverson, L. R.↗
NASA NTRS1993

High-resolution imaging gamma-ray spectroscopy with externally segmented germanium detectors

Externally segmented germanium detectors promise a breakthrough in gamma-ray imaging capabilities while retaining the superb energy resolution of germanium spectrometers. An angular resolution of 0.2 deg becomes practical by combining position-sensitive germanium detectors having a segment thickness of a few millimeters with a one-dimensional coded aperture located about a meter from the detectors. Correspondingly higher angular resolutions are possible with larger separations between the detectors and the coded aperture. Two-dimensional images can be obtained by rotating the instrument. Although the basic concept is similar to optical or X-ray coded-aperture imaging techniques, several complicating effects arise because of the penetrating nature of gamma rays. The complications include partial transmission through the coded aperture elements, Compton scattering in the germanium detectors, and high background count rates. Extensive electron-photon Monte Carlo modeling of a realistic detector/coded-aperture/collimator system has been performed. Results show that these complicating effects can be characterized and accounted for with no significant loss in instrument sensitivity.

Callas, J. L.↗