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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 145 records · Page 8

Range-wide population assessments for subalpine fir indicate widespread disturbance-driven decline

Subalpine forests in western North America are threatened by rapid climate change, increased activity by endemic and exotic insects and diseases, and changing wildfire regimes. The interactive effects of these stressors have resulted in pronounced population declines in many subalpine tree species; however, a systematic assessment of the status and trends of subalpine forests is lacking. Subalpine fir (Abies lasiocarpa) is a widespread species across the western United States, with documented population declines in many parts of its distribution. Here we use subalpine fir as an initial leverage point to build a more complete understanding of subalpine forest baseline conditions and responses to environmental change. Specifically, we leverage the USDA Forest Service Forest Inventory and Analysis (FIA) database to (1) ask how subalpine fir populations are changing across the species’ distribution in the western US, (2) assess the drivers of recent subalpine fir population trends, and (3) explore whether those changes imply generalized species-wide and/or system-wide decline. We found that subalpine fir abundance and basal area are declining concurrently across ~ 62% of the species’ distribution, and increasing across ~ 19%. Range-wide, we estimated 25.02 ± 2.74 % subalpine fir mortality between 2000 and 2009 and 2010–2019 FIA inventory periods, with higher mortality concentrated in the eastern Oregon Cascades, central Idaho, and parts of southern Colorado. High regeneration density did not predict positive population trajectories, which were instead associated with higher rates of adult recruitment. While the importance of different mortality agents varied substantially between ecoregions, 83.4% of total range-wide mortality was related to fire or biological disturbance. Declining subalpine fir basal area coincided with declines in the basal area of other co-occurring tree species in 39% of subalpine forest area, and with increases in conspecific basal area in 22% of forest area. Fire disturbance was the single largest cause of subalpine fir mortality; however, even where subalpine fir fire mortality was high, mortality among other species was primarily caused by insects. In conclusion, our results suggest that subalpine fir declines across large portions of the western United States are driven by forest disturbance, and that declines in subalpine fir populations may be indicative of negative change in subalpine forest systems broadly.

54 ENVIRONMENTAL SCIENCES↗

Salinity exposure affects lower-canopy specific leaf area of upland trees in a coastal deciduous forest

Sea level rise and increasing storm surges are likely to affect the canopy physiology, ecology, and structure of coastal forests, even well in advance of tree mortality. Laboratory and greenhouse studies have documented that saltwater exposure can trigger changes in leaf-level physiology and morphology, but few in situ studies have examined how tree-specific leaf area (SLA), the ratio of leaf area to mass and a crucial trait and model parameter, is affected by saline soils. We conducted an observational study of SLA in a mid-Atlantic (USA) coastal deciduous forest, taking advantage of a natural gradient in salinity along a tidal creek. Measured SLA of the 239 trees and seven species sampled ranged from Carya glabra (N = 6 trees, mean SLA = 277.9±36.3cm 2 /g) to Fagus grandifolia (N=60, 321.9±62.9cm 2 g); as expected, trees species and canopy position (sun versus shade) significantly affected SLA. For trees (N=100) directly exposed to the tidal creek, salinity was highly significant after accounting for species (P<0.001), with trees in the lower reaches of the creek having lower SLA. Leaf area index (LAI), computed from SLA and litter traps, ranged from 4.8 to 15.8 and was inversely related to salinity exposure; the spatial variability in leaf litter production contributed much more to LAI uncertainty than did SLA variability. These in situ results are correlative but consistent with the hypothesis, based on previous greenhouse studies, that the stress of chronic salinity exposure changes species’ leaf morphology. Our findings are useful for understanding the growing effects of saltwater intrusion into upland forests, as well as parameterizing and testing ecosystem-scale models simulating forest stressors and disturbances at the terrestrial-aquatic interface.

54 ENVIRONMENTAL SCIENCES↗

SoK: What does it Mean to Benchmark Database Forensics?

Relational Database Management Systems are the backbone of modern enterprises and public-sector services, and are thus frequent targets of security incidents, insider threats, and thorough regulatory audits. Consequently, databases have become key sources of digital evidence, requiring investigators to reconstruct past activity from audit logs, transaction logs, and backups. Although benchmarking frameworks such as those developed by the Transaction Processing Performance Council (TPC) are widely used to evaluate database performance, they do not capture forensic requirements such as evidentiary completeness, tamper-evidence, chain of custody, or regulatory compliance under GDPR and CCPA. This survey examines the emerging domain of forensic database benchmarking. We gathered prior research on database forensics, secure logging, and tamper-evident data structures; we analyze modern forensic-ready features in commercial and open-source systems (SQL Server Ledger, Oracle Blockchain Tables, PostgreSQL pgAudit, Db2 Audit, Aurora Database Activity Streams, Oracle Real Application Security and IBM Guardium) and assess why existing benchmarks are insufficient. We propose forensic workloads, metrics, and methodologies that incorporate adversarial stressors, deleted-record recovery, and backup analysis. We also identify open research problems and call for a community-driven forensic benchmark suite. The result is an idea for evaluating not only database performance but also forensic soundness, bridging the gap between system engineering, compliance, and digital investigations.

Lenard, Ben↗

Feasibility study for test rig assessments of fish passage conditions in a Kaplan turbine

The assessment of fish passage conditions in hydroelectric turbines consists of identifying and quantifying physical magnitudes leading to increased risks of injury of fish passing through turbines in operation. Such assessments are usually carried out either with the use of computer-based methods during design or with field testing of live fish and sensors passing through prototypes. A method in between consists of test rig experimentation, which is critical for testing fish-focused design concepts and offers the opportunity for implementing the most effective design measures for improved fish survivability. However, fish-related assessments in test rigs are not sufficiently documented for industrial applications. This work presents the main findings of an experimental campaign to quantify fish-related hydraulic magnitudes in a physical model of a Kaplan turbine in a commercial test rig. Two operating conditions were tested by releasing miniaturized autonomous sensor devices (termed Sensor Fish Mini) at the turbine intake flow, passing them through the runner in motion and recovering them at the draft tube exit. During passage, time series of acceleration, absolute pressure and rotational velocity were recorded. The recordings were then interpreted to determine the magnitude and likely location of hydraulic stressors hazardous to fish. The statistical tests on the reported measurements indicated that low pressure, collisions on the runner and rotations in the draft tube were not different between the two tested operating points. On the other hand, pressure drop and collision rates on the distributor differed considerably as a function of net head. The outcomes of this investigation showed that test rig evaluations of fish-related properties with Sensor Fish Mini can contribute to an evidence-based development of turbine geometries designed for providing safer passage conditions. Future work will investigate the scaling of test rig measurements to hydraulically equivalent magnitudes in the prototype and their biological consequences.

13 HYDRO ENERGY↗

The effect of electrical current on premature failures and microstructural degradation in bearing steel

Premature failures associated with microstructural degradation, i.e. white etching cracks (WECS), are becoming commonplace as applications are requiring tribological components to operate under extreme conditions. Although the specific drivers of WECs are still debated, the failures are often found in applications where cyclic loading is combined with added energy from stressors such as slip, impacts, or stray current. The aim of the current work is to elucidate effect that variable current has on premature failure. Numerous commercially available lubricants were tested, and both lubricant base oil type and additive package were found to have an effect on WEC failure time.

Bearing failure↗

Evolution of zygomycete secretomes and the origins of terrestrial fungal ecologies

Fungi survive in diverse ecological niches by secreting proteins and other molecules into the environment to acquire food and interact with various biotic and abiotic stressors. Fungal secretome content is, therefore, believed to be tightly linked to fungal ecologies. We sampled 132 genomes from the early-diverging terrestrial fungal lineage zygomycetes (Mucoromycota and Zoopagomycota) and characterized their secretome composition. Our analyses revealed that phylogeny played an important role in shaping the secretome composition of zygomycete fungi with trophic mode contributing a smaller amount. Reconstruction of the evolution of secreted digestive enzymes revealed lineage-specific expansions, indicating that Mucoromycota and Zoopagomycota followed different trajectories early in their evolutionary history. We identified the presence of multiple pathogenicity-related proteins in the lineages known as saprotrophs, suggesting that either the ecologies of these fungi are incompletely known, and/or that these pathogenicity-related proteins have important functions associated with saprotrophic ecologies, both of which invite further investigation.

59 BASIC BIOLOGICAL SCIENCES↗

The Elements of Life, Photosynthesis and Genomics

I am a Professor of Biochemistry, Biophysics and Structural Biology and Plant and Microbial Biology at the University of California in Berkeley. I was born and raised in India, emigrated to the United States to attend university, earning a B.S. in Molecular Biology and a Ph.D. in Biochemistry at the University of Wisconsin in Madison. Following post-doctoral studies with Lawrence Bogorad at Harvard University where I became interested in genetic control of trace element quotas, I joined the department of Chemistry and Biochemistry at UCLA. One of the first to appreciate essential trace metals as potential regulators of gene expression, I articulated the details of the nutritional Cu regulon in Chlamydomonas. In parallel, I used genetic approaches to discover the genes governing missing steps in tetrapyrrole metabolism, including the attachment of heme to apocytochromes in the thylakoid lumen and the factors catalyzing the formation of ring V in chlorophyll. After biochemistry and classical genetics, I embraced genomics, taking a leadership role on the Joint Genome Institute’s efforts on the Chlamydomonas genome and more recently, contributing to high quality assemblies of several genomes in the green algal radiation, and large transcriptomic and proteomic datasets — focusing on the diel metabolic cycle in synchronized cultures and acclimation to key environmental and nutritional stressors — that are well-used and appreciated by the community. Finally, a new venture in Berkeley is the promotion of Auxenochlorella protothecoides as the true “green yeast” and as a platform for engineering algae to produce useful bioproducts.

59 BASIC BIOLOGICAL SCIENCES↗

Phase-stabilized 2D/3D hetero-bilayers via lattice matching for efficient and stable inverted solar cells

2D-on-3D (2D/3D) perovskite heterostructures with engineered energy landscapes offer the potential to realize efficient and stable inverted solar cells. However, managing the energy landscape using 2D perovskites with thicker inorganic layers n > 1 necessitates the usage of the chemically unstable methylammonium MA + . Here, we synthesized formamidinium (FA)-rich and pure-FA n = 3 Ruddlesden-Popper (RPP) and Dion-Jacobson perovskite (DJP) single crystals by identifying ligands with suitably lattice-matched organic and inorganic components of the 2D lattice. These crystals were translated onto 3D perovskites as capping layers, forming 2D/3D hetero-bilayers (HBs). Degradation studies revealed that HBs with butylammonium-based RPPs as capping layers rapidly phase segregate into non-perovskites under combined extrinsic stressors, compromising the underlying 3D layer, whereas FA-rich DJPs based on 3-aminomethylpiperidine retain their phase stability. The DJP HBs also possess a favorable energy landscape and electron transport at the 2D/3D interface, enabling inverted solar cells with a champion PCE of 25.33% and remarkable stability.

77 NANOSCIENCE AND NANOTECHNOLOGY↗

Fortifying the frontier: cell wall modifications during plant immunity

The plant cell wall (CW) was long thought to be a rigid barrier encasing the plant cell and protecting it against biotic and abiotic stressors. Different CW polysaccharides interact with each other, and modifications of either the components or organization of these polysaccharides result in impaired growth or immunity. Emerging evidence suggests that the CW is dynamically modified and reorganized based on internal and external cues. Thus, the CW is both the first barrier that pathogens encounter and the critical final step in defense signaling that leads to fortification of the CW. Here, in this work, we review recent findings on how CW components are remodeled to fortify the CW upon pathogen attack and propose a novel concept: layered CW remodeling as an immune strategy. Within this framework, we categorize three interconnected layers of CW remodeling upon pathogen attack: (i) rapid and reversible CW depositions that provide immediate but transient protection; (ii) flexible modifications with plausible signaling functions that integrate defense and surveillance; and (iii) irreversible fortifications that encase pathogen, delimiting infected cells from uninfected cells. This layered framework provides a cohesive view of how different CW modifications are integrated into, and contribute to, plant defense. We also discuss the challenges in studying CW modifications during biotic stresses and highlight important questions that remain unanswered.

Bhandari, Deepak D. [Michigan State Univ., East La↗

PLAT domain protein 1 (PLAT1/PLAFP) binds to the Arabidopsis thaliana plasma membrane and inserts a lipid

Robust agricultural yields depend on the plant's ability to fix carbon amid variable environmental conditions. Over seasonal and diurnal cycles, the plant must constantly adjust its metabolism according to available resources or external stressors. The metabolic changes that a plant undergoes in response to stress are well understood, but the long-distance signaling mechanisms that facilitate communication throughout the plant are less studied. The phloem is considered the predominant conduit for the bidirectional transport of these signals in the form of metabolites, nucleic acids, proteins, and lipids. Lipid trafficking through the phloem in particular attracted our attention due to its reliance on soluble lipid-binding proteins (LBP) that generate and solubilize otherwise membrane-associated lipids. The Phloem Lipid-Associated Family Protein (PLAFP) from Arabidopsis thaliana is generated in response to abiotic stress as is its lipid-ligand phosphatidic acid (PA). PLAFP is proposed to transport PA through the phloem in response to drought stress. To understand the interactions between PLAFP and PA, nearly 100 independent systems comprised of the protein and one PA, or a plasma membrane containing varying amounts of PA, were simulated using atomistic classical molecular dynamics methods. In these simulations, PLAFP is found to bind to plant plasma membrane models independent of the PA concentration. When bound to the membrane, PLAFP adopts a binding pose where W41 and R82 penetrate the membrane surface and anchor PLAFP. This triggers a separation of the two loop regions containing W41 and R82. Subsequent simulations indicate that PA insert into the β -sandwich of PLAFP, driven by interactions with multiple amino acids besides the W41 and R82 identified during the insertion process. Finally, fine-tuning the protein-membrane and protein-PA interface by mutating a selection of these amino acids may facilitate engineering plant signaling processes by modulating the binding response.

59 BASIC BIOLOGICAL SCIENCES↗

Using electronic health record metadata to predict housing instability amongst veterans

Housing instability is considered a significant life stressor and preemptive screening should be applied to identify those at risk for homelessness as early as possible so that they can be targeted for specialized care. We developed models to classify patient outcomes for an established VA Homelessness Screening Clinical Reminder (HSCR), which identifies housing instability, in the two months prior to its administration. Logistic Regression and Random Forest models were fit to classify responses using the last 18 months of document activity. We measure concentration of risk across stratifications of predicted probability and observe an enriched likelihood of finding confirmed false negative responses from veterans with diagnosed housing instability. Positive responses were 34 times more likely to be detected within the top 1 % of patients predicted at risk than from those randomly selected. There is a 1 in 4 chance of detecting false negatives within the top 1 % of predicted risk. Machine learning methods can classify between episodes of housing instability using a data-driven approach that does not rely on variables curated from domain experts. This method has the potential to improve clinicians’ ability to identify veterans who are experiencing housing instability but are not captured by HSCR.

60 APPLIED LIFE SCIENCES↗

Diffusion-limited hydrolysis in polymeric materials

Polymer degradation under aggressive environmental stressors often develops heterogeneities due to diffusion-limited reaction phenomena. This is well established for diffusion-limited oxidation (DLO), which is known to occur for most polymeric materials at elevated temperatures but has been less summarized for the conditions of diffusion-limited hydrolysis (DLH). Here, an overview of hydrolysis for several materials and a computational model, analogous to the underlying equations for DLO, able to define this diffusion-reaction system is presented. A systematic study of the influence of various parameters, such as water diffusivity, reaction rate and order, and a more in-depth focus on residual isocyanate hydrolysis in a polymeric methylene diphenyl diisocyanate (pMDI) based polyurethane (PU) foam is given. For this system, we present experimental data for model ‘input’ parameters and discuss predictions for different conditions. We conceptually compare the behavior of diffusion-limited oxidation to that of diffusion-limited hydrolysis (DLH). With the mathematical framework and key material properties presented herein, any DLH phenomena following Fickian diffusion behavior can be understood, modeled, and predicted.

36 MATERIALS SCIENCE↗

Hygrothermal aging effects on polyimide and acrylate-based adhesive materials in high-performance flat-flex cable assemblies

Flat-flex assemblies are widely used across a diverse range of technologies, including consumer electronics, automotive systems, aerospace and defense applications, and medical devices, due to their compact form factor and flexibility. However, environmental stressors like temperature and humidity are known to degrade performance over time, though the main mechanisms for degradation remain unknown. Here, in this work, we examined the aging behavior of copper/polyimide/adhesive laminate systems under varying temperature and humidity conditions, focusing on material degradation and its effects on mechanical and dielectric properties. Results show that peel strength and breakdown voltage decrease with exposure time, temperature, and humidity; spectroscopic characterization revealed that the adhesive, and not the polyimide, is the weak link in the material stack-up. The adhesive, identified as a butyl acrylate-acrylonitrile (BA-AN) copolymer, exhibited age-related spectral changes that correlated with exposure severity and duration. Hydrolysis at BA ester and AN nitrile groups was identified as the primary degradation mechanism, producing amides, acids, and alcohols. We developed a mechanistic model that connects kinetic parameters for BA-AN spectral band decay to peel strength and breakdown voltage, and implicates hydrolysis at the AN moiety, not BA, in performance decrements. Modeling predictions at ambient conditions indicate faster decline in peel strength than dielectric strength at early times, followed by plateauing of both properties at longer times. These findings provide critical insights into laminate aging mechanisms and highlight the importance of addressing BA-AN degradation to improve the long-term reliability of these systems in high-humidity environments.

organic↗

Climate-eutrophication-anoxia interactions in Late Glacial Soppensee, Switzerland: Forcings, non-linear responses and recovery

Combined effects of climate warming and anthropogenic nutrient loadings lead to lake eutrophication and anoxia globally. Because of chemical feedbacks, lakes under multiple stressors often respond in non-linear ways. However, it remains unclear whether climate change alone can lead to non-linear lake responses in the absence of anthropogenic nutrient disturbances. Here, we investigate the interactions between climate variability, nutrient cycling and trophic state changes, mixing regimes, anoxia and related chemical feedback in a small kettle-hole lake in Switzerland during Late Glacial times (15.2–12.6 cal ka BP), a period known for high-amplitude climate change in pre-anthropogenic times. After its formation during Heinrich Stadial 1 (>15 cal ka BP), Soppensee was oligotrophic and well-mixed. Soppensee became eutrophic and developed anoxia at 14.25 cal ka BP. Phosphorus (P) was released from sediments through the reductive dissolution of Fe-oxyhydroxides, fuelling eutrophication. Eutrophication lagged the Bølling warming (14.65 cal ka BP) by 400 years, suggesting that rising temperatures were not the trigger for eutrophication. Instead, eutrophication responded non-linearly to forest closure (threshold at 76 % arboreal pollen AP), which shielded Soppensee from wind mixing, enhancing lake stratification, anoxia and P release, intensifying eutrophication. These conditions ended during the 200-years cold period of the Aegelsee Oscillation (GI-1d, ca. 14.0 cal ka BP) when the landscape regionally opened (AP<76 %); the lake became well-mixed, oxygenated and P was efficiently sequestered. Throughout the Allerød (13.9–12.8 cal ka BP), enhanced Fe input prompted diagenetic vivianite formation, sequestering P in sediments, naturally remediating lake eutrophication despite closed forests, warm temperatures, lake stratification and anoxia.

Environmental sciences↗

Disruption of the endogenous indole glucosinolate pathway impacts the Arabidopsis thaliana root exudation profile and rhizobacterial community

Root exudates are composed of primary and secondary metabolites known to modulate the rhizosphere microbiota. Glucosinolates are defense compounds present in the Brassicaceae family capable of deterring pathogens, herbivores and biotic stressors in the phyllosphere. In addition, traces of glucosinolates and their hydrolyzed byproducts have been found in the soil, suggesting that these secondary metabolites could play a role in the modulation and establishment of the rhizosphere microbial community associated with this family. Here, we used Arabidopsis thaliana mutant lines, including the cyp79B2cyp79B3 double mutant line with a disruption in the indole glucosinolate pathway and atr1D, which overexpresses ATR1 and increases glucosinolate production. These lines were analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and 16S rRNA amplicon sequencing to evaluate how genetic modifications to the indole glucosinolate pathway affects the root exudate profile of Arabidopsis thaliana, and, in turn, impacts the rhizosphere microbial community. Metabolic analysis of root exudates from the wild-type Columbia (Col-0), along with the mutant lines, confirmed that alterations to the indole glucosinolate biosynthetic pathway result in shifts in the root exudate profile of the plant. We observed changes in the relative abundance of exuded metabolites. Moreover, 16S rRNA amplicon sequencing results provided evidence that the rhizobacterial communities associated with the plant lines used were directly impacted in diversity and community composition. Here, this work provides further information on the involvement of secondary metabolites and their role in modulating the rhizobacterial community. Root metabolites dictate the presence of different bacterial species, including plant growth-promoting rhizobacteria (PGPR). Our results suggest that genetic alterations in the indole glucosinolate pathway cause disruptions beyond the endogenous levels of the plant, significantly changing the abundance and presence of different metabolites in the root exudates of the plants as well as the microbial rhizosphere community.

59 BASIC BIOLOGICAL SCIENCES↗

Integration of ecosystem science into radioecology: A consensus perspective

In the Fall of 2016 a workshop was held which brought together over 50 scientists from the ecological and radiological fields to discuss feasibility and challenges of reintegrating ecosystem science into radioecology. There is a growing desire to incorporate attributes of ecosystem science into radiological risk assessment and radioecological research more generally, fueled by recent advances in quantification of emergent ecosystem attributes and the desire to accurately reflect impacts of radiological stressors upon ecosystem function. This work is a synthesis of the discussions and consensus of the workshop participant's responses to three primary questions, which were: 1) How can ecosystem science support radiological risk assessment? 2) What ecosystem level endpoints potentially could be used for radiological risk assessment? and 3) What inference strategies and associated methods would be most appropriate to assess the effects of radionuclides on ecosystem structure and function? The consensus of the participants was that ecosystem science can and should support radiological risk assessment through the incorporation of quantitative metrics that reflect ecosystem functions which are sensitive to radiological contaminants. The participants also agreed that many such endpoints exit or are thought to exit and while many are used in ecological risk assessment currently, additional data need to be collected that link the causal mechanisms of radiological exposure to these endpoints. Finally, the participants agreed that radiological risk assessments must be designed and informed by rigorous statistical frameworks capable of revealing the causal inference tying radiological exposure to the endpoints selected for measurement.

54 ENVIRONMENTAL SCIENCES↗

Autonomous sensor suite for evaluating fish-turbine interactions and environmental impacts in marine renewable energy and hydropower

Marine renewable energy (MRE) harnesses ocean-based resources such as waves, tides, currents, and thermal or salinity gradients for sustainable power generation. It has the potential to complement existing renewable resources, support remote communities, and contribute to decarbonization efforts. However, understanding the hydrodynamic forces created by MRE devices and their impacts on marine life is critical for responsible deployment. Here, to address these concerns, advanced sensor devices, including the Marine Sensor Fish (MSF), Sensor Fish Mini (SF Mini), and Flexible Sensor Fish (FSF), were developed to measure interactions between aquatic organisms and MRE systems. This paper details the design, manufacturing, calibration, and field deployment of these sensor suites, highlighting their ability to capture key physical stressors such as shear forces, pressure changes, and collision impacts. The MSF successfully evaluated turbine interactions at a tidal turbine in the Salish Sea, capturing data on turbulence, collision impact, and pressure gradients. The SF Mini validated hydrodynamic conditions in scaled hydraulic models, supporting computational fluid dynamics simulations. The FSF, with its flexible silicone body, measured species-specific impacts in turbulent environments. This research demonstrates the potential of Sensor Fish technology to advance sustainable marine energy systems by reducing biological impacts and informing environmentally sustainable designs.

Ecological impacts↗