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NASA NTRS2001

Combining New Satellite Tools and Models to Examine Role of Mesoscale Interactions in Formation and Intensification of Tropical Cyclones

The objective of this research is to start filling the mesoscale gap to improve understanding and probability forecasts of formation and intensity variations of tropical cyclones. Sampling by aircraft equipped to measure mesoscale processes is expensive, thus confined in place and time. Hence we turn to satellite products. This paper reports preliminary results of a tropical cyclone genesis and early intensification study. We explore the role of mesoscale processes using a combination of products from TRMM, QuikSCAT, AMSU, also SSM/I, geosynchronous and model output. Major emphasis is on the role of merging mesoscale vortices. These initially form in midlevel stratiform cloud. When they form in regions of lowered Rossby radius of deformation (strong background vorticity) the mesoscale vortices can last long enough to interact and merge, with the weaker vortex losing vorticity to the stronger, which can then extend down to the surface. In an earlier cyclongenesis case (Oliver 1993) off Australia, intense deep convection occurred when the stronger vortex reached the surface; this vortex became the storm center while the weaker vortex was sheared out as the major rainband. In our study of Atlantic tropical cyclones originating from African waves, we use QuikSCAT to examine surface winds in the African monsoon trough and in the vortices which move westward off the coast, which may or may not undergo genesis (defined by NHC as reaching TD, or tropical depression, with a west wind to the south of the surface low). We use AMSU mainly to examine development of warm cores. TRMM passive microwave TMI is used with SSM/I to look at the rain structure, which often indicates eye formation, and to look at the ice scattering signatures of deep convection. The TRMM precipitation radar, PR, when available, gives precipitation cross sections. So far we have detailed studies of two African-origin cyclones, one which became severe hurricane Floyd 1999, and the other reached TD2 in June 2000 and then died out. The atmosphere off West African is dry and stable. It becomes less so between June and September, as the SST and convection heat up. QuikSCAT shows the African monsoon trough and shear zone extend westward over the ocean to nearly 30 degrees West. The evidence is strong that the two cyclones had in common multiple midlevel mergers, which extended to the surface keeping the surface vortex strong. These continued until both systems were designated TD's by NHC. In the June 2000 case, the main reason for failure was the lower SST and dry, stable atmosphere. This is shown by the comparison of the equivalent potential temperature maps and profiles with those from pre-Floyd. In the vortex which became Floyd, QuikSCAT shows continuous importation of high theta e (warm, moist) air from the south. From September 2-8, this air flowed around the vortex center, building up a high theta-e pool to the north. Then late on September 9, a 100-km wide jet of high theta-e air penetrated the vortex core, a major convective burst' was observed, and an intensifying, more elevated warm core was seen on AMSU. Rapid pressure fall and wind intensification were underway by 0000 UTC on September 10. Floyd became a Hurricane at 1200 UTC on Sept 10, 1999, with successive convective bursts running the hurricane thermodynamic engine by intensifying the warm core. TD2 was a strong African vortex, sustained by moderate convection (up to about 12.5 km) offshore of Africa. It peaked on June 23, showing an apparent "eye" on passive microwave composites. However, it could not assemble the ingredients for a convective burst. Thus it failed to get the thermodynamic hurricane engine going before it moved too far west of the region of lowered Rossby radius. By June 26, cloud systems were dying out. On June 25, a surface vortex was no longer seen on QuikSCAT, although one continued above the surface on model profiles until June 27. One of our main findings so far is showing the role of the mesoscale vortex interactions in sustaining some African vortices far out in to the mid Atlantic, where under adequate thermal/moisture conditions the hurricane heat engine can sometimes be started. We are working on similar studies of Cindy and Irene 1999. Cindy illustrates a case of wind shear working against an early-stage hurricane heat engine, while Irene formed from a Caribbean wave. An enormous value of combinations of satellite tools is that tropical cyclones can be studied in all parts of the global oceans where they occur. Detailed studies like ours are labor intensive but many statistical studies can be based on physical postulates developed. There are other new tools such as MODIS on TERRA of the Earth Observing System (EOS) which can be used to study the microphysics of tropical cyclones world wide, in particular to investigate the presence of mixed phase and the impact of atmospheric aerosols on the hydrometeor structure and rainfall from tropical cyclones.

Simpson, Joanne↗

NASA NTRS2005

Evaluation of the Simulation of Arctic and Antarctic Sea Ice Coverages by Eleven Major Global Climate Models

Comparison of polar sea ice results from 11 major global climate models and satellite-derived observations for 1979-2004 reveals that each of the models is simulating seasonal cycles that are phased at least approximately correctly in both hemispheres. Each is also simulating various key aspects of the observed ice cover distributions, such as winter ice not only throughout the central Arctic basin but also throughout Hudson Bay, despite its relatively low latitudes. However, some of the models simulate too much ice, others too little ice (in some cases varying depending on hemisphere and/or season), and some match the observations better in one season versus another. Several models do noticeably better in the Northern Hemisphere than in the Southern Hemisphere, and one does noticeably better in the Southern Hemisphere. In the Northern Hemisphere all simulate monthly average ice extents to within +/-5.1 x 10(exp 6)sq km of the observed ice extent throughout the year; and in the Southern Hemisphere all except one simulate the monthly averages to within +/-6.3 x 10(exp 6) sq km of the observed values. All the models properly simulate a lack of winter ice to the west of Norway; however, most do not obtain as much absence of ice immediately north of Norway as the observations show, suggesting an under simulation of the North Atlantic Current. The spread in monthly averaged ice extents amongst the 11 model simulations is greater in the Southern Hemisphere than in the Northern Hemisphere and greatest in the Southern Hemisphere winter and spring.

Parksinson, Claire↗

DOE OSTI2022

Increasing Hurricane Intensification Rate Near the US Atlantic Coast

Hurricanes often cause severe damage and loss of life, and storms that intensify close to the coast pose a particularly serious threat. While changes in hurricane intensification and environment have been examined at basin scales previously, near-coastal changes have not been adequately explored. In this study, we address this using a suite of observations and climate model simulations. Over the 40-year period of 1979–2018, the mean 24-hr hurricane intensification rate increased by ~1.2 kt 6-hr –1 near the US Atlantic coast. However, a significant increase in intensification did not occur near the Gulf coast over the same period. The enhanced hurricane intensification along the Atlantic coast is consistent with an increasingly favorable dynamic and thermodynamic environment there, which is well simulated by climate models over the historical period. Further, multi-model projections suggest a continued enhancement of the storm environment and hurricane intensification near the Atlantic coast in the future.

54 ENVIRONMENTAL SCIENCES↗

NASA NTRS2018

Downscaling Atmosphere-Ocean Global Climate Model Precipitation Simulations over Africa Using Bias-Corrected Lateral and Lower Boundary Conditions

A prequel study showed that dynamic downscaling using a regional climate model (RCM) over Africa improved the Goddard Institute for Space Studies Atmosphere-Ocean Global Climate Model (GISS AOGCM: ModelE) simulation of June–September rainfall patterns over Africa. The current study applies bias corrections to the lateral and lower boundary data from the AOGCM driving the RCM, based on the comparison of a 30-year simulation to the actual climate. The analysis examines the horizontal pattern of June–September total accumulated precipitation, the time versus latitude evolution of zonal mean West Africa (WA) precipitation (showing monsoon onset timing), and the latitude versus altitude cross-section of zonal winds over WA (showing the African Easterly Jet and the Tropical Easterly Jet). The study shows that correcting for excessively warm AOGCM Atlantic sea-surface temperatures (SSTs) improves the simulation of key features, whereas applying 30-year mean bias corrections to atmospheric variables driving the RCM at the lateral boundaries does not improve the RCM simulations. We suggest that AOGCM climate projections for Africa should benefit from downscaling by nesting an RCM that has demonstrated skill in simulating African climate, driven with bias-corrected SST.

West African monsoon↗

NASA NTRS1989

Low frequency oscillations in total ozone measurements

Low frequency oscillations with periods of approximately one to two months are found in eight years of global grids of total ozone data from the Total Ozone Mapping Spectrometer (TOMS) satellite instrument. The low frequency oscillations corroborate earlier analyses based on four years of data. In addition, both annual and seasonal one-point correlation maps based on the 8-year TOMS data are presented. The results clearly show a standing dipole in ozone perturbations, oscillating with 35 to 50 day periods over the equatorial Indian Ocean-west Pacific region. This contrasts with the eastward moving dipole reported in other data sets. The standing ozone dipole appears to be a dynamical feature associated with vertical atmospheric motions. Consistent with prior analyses based on lower stratospheric temperature fields, large-scale standing patterns are also found in the extratropics of both hemispheres, correlated with ozone fluctuations over the equatorial west Pacific. In the Northern Hemisphere, a standing pattern is observed extending from the tropical Indian Ocean to the north Pacific, across North America, and down to the equatorial Atlantic Ocean region. This feature is most pronounced in the NH summer.

Gao, X. H.↗

NASA NTRS1990

Low-frequency oscillations in total ozone measurements

Gao, X. H.↗

DOE OSTI2023

The Influence of Soil Properties on Sea-Breeze Circulations in the Southeast U.S.

Sea-breeze circulations (SBCs) are common weather phenomena at and near coastal regions. They form because of a thermal gradient between the land surface at the coast and the sea surface. In a mid-day regime, a “thermal low” generated at the warm coast will lead to rising air motion, creating a wind shift coming from the sea near the surface displacing the coastal air. A “return flow” moving back towards the sea is generated by upper-level divergence because of the rising motion from the thermal low. SBCs propagate and serve as a method of urban pollutant dispersion in the Los Angeles region of California and are constrained to the coast due to the topography of surrounding mountains serving as a boundary for further inland propagation. Within the northeast U.S. SBCs are seen in the warm season but tend to remain coastally bound due to Coriolis distortion over long distances. Within the southeast U.S. (SEUS), the paradigmatic example of SBCs occurs over the Florida peninsula, where thunderstorms form on a nearly daily occurrence due to the convergence of SBCs from the east and west sides of the peninsula. However, there are further examples of sea-breezes in the SEUS that warrant study. Within the region bordering the SEUS and the Mid-Atlantic, just east of the southern Appalachian Mountains, warm-season SBCs form at the coast of Georgia and the Carolinas. Relatively flat topography ~150-200km inland allows for mostly unimpeded inland SBC propagation. Through visual analysis, Viner et al. catalogued several SBCs that propagated as far inland as the Central Savannah River Area surrounding Augusta, Georgia. Wermter et al. found that while the land-sea thermal gradient at the coast can influence coastal SBC genesis, the inland-coastal thermal gradient over the land is the primary influencer on the speed and depth of inland propagation of SBCs in this region. Additionally, soil moisture itself is a known correlative factor to sea-breeze formation, as it influences the soil temperature and the thermal gradient needed for SBC genesis and inland penetration. Physick determined that higher latent heat fluxes associated with wetter soil dampen the land-sea thermal gradient and suppress the formation of a SBC. Physick determined that higher latent heat fluxes associated with wetter soil dampen the land-sea thermal gradient and suppress the formation of a SBC. Conversely, drier soil enhances the thermal gradient and promotes SBC formation. However, while there is an inverse relationship between soil moisture and SBCs, higher soil moisture can actually promote more convective rainfall following a SBC if it does not significantly impact the thermal gradient. While the relationship between soil moisture and SBC formation has been conceptually explored and modeled numerically, there is a research gap in observed connections. The Soil Moisture Active Passive (SMAP) satellite mission has been operational since 2015 and has been used to create high-resolution re-analytical Level 4 (L4) datasets of soil moisture and soil temperature at different soil depths: the surface (0-5cm) and rootzone (0-1m). The surface soil temperature effectively acts as the “skin temperature” of the surface at these levels, and a spatial map of the land-sea as well as the coastal-inland thermal gradients can be represented. SMAP data are also assimilated in some atmospheric models such at the High Resolution Rapid Refresh (HRRR) mesoscale model. We propose leveraging the use of SMAP products to fill in spatial gaps left by weather and mesonet stations within the SEUS region, as well as assessing the effectiveness of utilizing SMAP products towards SBC forecasting in both deterministic and machine learning (ML) models.

58 GEOSCIENCES↗

NASA NTRS1988

Results of APL rain gauge network measurements in mid-Atlantic coast region and comparisons of distributions with CCIR models

In this effort are described cumulative rain rate distributions for a network of nine tipping bucket rain gauge systems located in the mid-Atlantic coast region in the vicinity of the NASA Wallops Flight Facility, Wallops Island, Virginia. The rain gauges are situated within a gridded region of dimensions of 47 km east-west by 70 km north-south. Distributions are presented for the individual site measurements and the network average for the year period June 1, 1986 through May 31, 1987. A previous six year average distribution derived from measurements at one of the site locations is also presented. Comparisons are given of the network average, the CCIR (International Radio Consultative Committee) climatic zone, and the CCIR functional model distributions, the latter of which approximates a log normal at the lower rain rate and a gamma function at the higher rates.

Goldhirsh, Julius↗

NASA NTRS2009

An Analysis of Moisture Fluxes into the Gulf of California

This study examines the nature of episodes of enhanced warm-season moisture flux into the Gulf of California. Both spatial structure and primary time scales of the fluxes are examined using the 40-yr ECMWF Re-Analysis data for the period 1980-2001. The analysis approach consists of a compositing technique that is keyed on the low-level moisture fluxes into the Gulf of California. The results show that the fluxes have a rich spectrum of temporal variability, with periods of enhanced transport over the gulf linked to African easterly waves on subweekly (3-8 day) time scales, the Madden-Julian oscillation (MJO) at intraseasonal time scales (20-90 day), and intermediate (10-15 day) time-scale disturbances that appear to originate primarily in the Caribbean Sea-western Atlantic Ocean. In the case of the MJO, enhanced low-level westerlies and large-scale rising motion provide an environment that favors large-scale cyclonic development near the west coast of Central America that, over the course of about 2 weeks, expands northward along the coast eventually reaching the mouth of the Gulf of California where it acts to enhance the southerly moisture flux in that region. On a larger scale, the development includes a northward shift in the eastern Pacific ITCZ, enhanced precipitation over much of Mexico and the southwestern United States, and enhanced southerly/southeasterly fluxes from the Gulf of Mexico into Mexico and the southwestern and central United States. In the case of the easterly waves, the systems that reach Mexico appear to redevelop/reorganize on the Pacific coast and then move rapidly to the northwest to contribute to the moisture flux into the Gulf of California. The most intense fluxes into the gulf on these time scales appear to be synchronized with a midlatitude short-wave trough over the U.S. West Coast and enhanced low-level southerly fluxes over the U.S. Great Plains. The intermediate (10-15 day) time-scale systems have zonal wavelengths roughly twice that of the easterly waves, and their initiation appears to be linked to an extratropical U.S. East Coast ridge and associated northeasterly winds that extend well into the Caribbean Sea during their development phase. The short (3-8 day) and, to a lesser extent, the intermediate (10-15 day) time-scale fluxes tend to be enhanced when the convectively active phase of the MJO is situated over the Americas.

Wu, Man-Li C.↗

NASA NTRS1994

Evolution of upwelling fronts off the coast of Portugal

We have analyzed the spatial and temporal variability of chlorophyll in the Atlantic Ocean off the Portugal coast using satellite imagery from 1979 to 1986 in conjunction with wind data. The spatial patterns in the imagery show the typical inshore-offshore and east-west gradients, while the temporal patterns show seasonal high winter values and low summer values. In the satellite imagery for the period 1979-1986, stable mesoscale and large scale patterns recurred despite interannual differences in absolute satellite-derived pigment concentrations.

Source record↗

DOE OSTI2021

Assessing Global Ocean Circulation Sensitivity to Changes in Antarctic Ice Discharge

The principal goal of this research is to assess how projected increases in meltwater discharge and iceberg calving from the Antarctic Ice Sheet will impact climate in the next 250 years. In short, the sensitivity of the global ocean circulation and climate, and in particular the Atlantic Meridional Overturning Circulation (AMOC), to large increases in iceberg calving and meltwater discharges to the Southern Ocean are poorly understood. The requirement to investigate this topic is heightened by growing evidence that the West Antarctic Ice Sheet (WAIS) is vulnerable to rapid retreat and collapse through marine-ice-sheet-instability (MISI) on multidecadal-to-centennial timescales. Future model simulations of the AIS show the potential for rapid ice sheet retreat in the next 50 – 300 years, with such a collapse associated with the discharge of enormous volumes of ice and meltwater to the Southern Ocean. This project uses a combination of an ice sheet/shelf model and a fully coupled (ocean-atmosphere-seaice) model (CESM 1.2) to explore the sensitive of global climate to future changes in runoff from the Antarctic continent under future greenhouse gas emissions scenarios IPCC RCP4.5 and 8.5. Overall, accounting for Antarctic meltwater reduces the projected global mean anthropogenic greenhouse warming by up to 2 deg C. during peak WAIS collapse and by ~1 deg. for most of the simulation. In addition, the increased meltwater contribution raises sub-surface ocean temperatures at the Antarctic ice sheet margin by more than 1ºC, such that the ice shelf basal melt rates increase beyond those currently projected. In contrast, the model predicts a strong cooling of both the surface air and ocean temperatures over the Southern Ocean that results in a massive expansion and thickening of the perennial sea-ice. The slow-down in anthropogenic warming also results in the onset of ice-free conditions in the Arctic being delayed in the model by several decades, and the projected slowdown in the AMOC to reduce.

58 GEOSCIENCES↗

NASA NTRS2000

Radiative Properties of Smoke and Aerosol Over Land Surfaces

This talk discusses smoke and aerosol's radiative properties with particular attention to distinguishing the measurement over clear sky from clouds over land, sea, snow, etc. surfaces, using MODIS Airborne Simulator data from (Brazil, arctic sea ice and tundra and southern Africa, west Africa, and other ecosystems. This talk also discusses the surface bidirectional reflectance using Cloud Absorption Radiometer, BRDF measurements of Saudi Arabian desert, Persian Gulf, cerrado and rain forests in Brazil, sea ice, tundra, Atlantic Ocean, Great Dismal Swamp, Kuwait oil fire smoke. Recent upgrades to instrument (new TOMS UVA channels at 340 and 380 planned use in Africa (SAFARI 2000) and possibly for MEIDEX will also be discussed. This talk also plans to discuss the spectral variation of surface reflectance over land and the sensitivity of off-nadir view angles to correlation between visible near-infrared reflectance for use in remote sensing of aerosol over land.

King, Michael D.↗

NASA NTRS2012

Southern Hemisphere Additional Ozonesondes (SHADOZ) Ozone Climatology (2005-2009): Tropospheric and Tropical Tropopause Layer (TTL) Profiles with Comparisons to Omi-based Ozone Products

We present a regional and seasonal climatology of SHADOZ ozone profiles in the troposphere and tropical tropopause layer (TTL) based on measurements taken during the first five years of Aura, 2005-2009, when new stations joined the network at Hanoi, Vietnam; Hilo, Hawaii; Alajuela Heredia, Costa Rica; Cotonou, Benin. In all, 15 stations operated during that period. A west-to-east progression of decreasing convective influence and increasing pollution leads to distinct tropospheric ozone profiles in three regions: (1) western Pacific eastern Indian Ocean; (2) equatorial Americas (San Cristobal, Alajuela, Paramaribo); (3) Atlantic and Africa. Comparisons in total ozone column from soundings, the Ozone Monitoring Instrument (OMI, on Aura, 2004-) satellite and ground-based instrumentation are presented. Most stations show better agreement with OMI than they did for EPTOMS comparisons (1998-2004; Earth-ProbeTotal Ozone Mapping Spectrometer), partly due to a revised above-burst ozone climatology. Possible station biases in the stratospheric segment of the ozone measurement noted in the first 7 years of SHADOZ ozone profiles are re-examined. High stratospheric bias observed during the TOMS period appears to persist at one station. Comparisons of SHADOZ tropospheric ozone and the daily Trajectory-enhanced Tropospheric Ozone Residual (TTOR) product (based on OMIMLS) show that the satellite-derived column amount averages 25 low. Correlations between TTOR and the SHADOZ sondes are quite good (typical r2 0.5-0.8), however, which may account for why some published residual-based OMI products capture tropospheric interannual variability fairly realistically. On the other hand, no clear explanations emerge for why TTOR-sonde discrepancies vary over a wide range at most SHADOZ sites.

gravity waves↗

NASA NTRS2016

Aerosol-Radiation-Cloud Interactions in the South-East Atlantic: First Results from the ORACLES-2016 Deployment and Plans for Future Activities

Southern Africa produces almost a third of the Earths biomass burning (BB) aerosol particles. Particles lofted into the mid-troposphere are transported westward over the South-East (SE) Atlantic, home to one of the three permanent subtropical stratocumulus (Sc) cloud decks in the world. The SE Atlantic stratocumulus deck interacts with the dense layers of BB aerosols that initially overlay the cloud deck, but later subside and may mix into the clouds. These interactions include adjustments to aerosol-induced solar heating and microphysical effects, and their global representation in climate models remains one of the largest uncertainties in estimates of future climate. Hence, new observations over the SE Atlantic have significant implications for regional and global climate change predictions.Our understanding of aerosol-cloud interactions in the SE Atlantic is severely limited. Most notably, we are missing knowledge on the absorptive and cloud nucleating properties of aerosols, including their vertical distribution relative to clouds, on the locations and degree of aerosol mixing into clouds, on the processes that govern cloud property adjustments, and on the importance of aerosol effects on clouds relative to co-varying synoptic scale meteorology.We describe first results from various synergistic, international research activities aimed at studying aerosol-cloud interactions in the region:NASAs airborne ORACLES (ObseRvations of Aerosols Above Clouds and Their IntEractionS) deployment in AugustSeptember of 2016,the DoEs LASIC (Layered Atlantic Smoke Interactions with Clouds) deployment of the ARM Mobile Facility to Ascension Island (June 2016 October 2017), the ground-based components of CNRS AEROCLO-sA (Aerosols Clouds and Fog over the west coast of southern Africa), and ongoing regional-scale integrative, process-oriented science efforts as part of SEALS-sA (Sea Earth Atmosphere Linkages Study in southern Africa).We expect to describe experimental setups as well as showcase initial aerosol and cloud property distributions. Furthermore, we discuss the implementation of future activities in these programs in coordination with the UK Met Offices CLARIFY (CLoud-Aerosol-Radiation Interactions and Forcing) experiment in 2017.

aerosol↗

DOE OSTI2023

Ocean current patterns drive the worldwide colonization of eelgrass (Zostera marina)

Currents are unique drivers of oceanic phylogeography and thus determine the distribution of marine coastal species, along with past glaciations and sea-level changes. Here we reconstruct the worldwide colonization history of eelgrass (Zostera marina L.), the most widely distributed marine flowering plant or seagrass from its origin in the Northwest Pacific, based on nuclear and chloroplast genomes. We identified two divergent Pacific clades with evidence for admixture along the East Pacific coast. Two west-to-east (trans-Pacific) colonization events support the key role of the North Pacific Current. Time-calibrated nuclear and chloroplast phylogenies yielded concordant estimates of the arrival of Z. marina in the Atlantic through the Canadian Arctic, suggesting that eelgrass-based ecosystems, hotspots of biodiversity and carbon sequestration, have only been present there for ~243 ky (thousand years). Mediterranean populations were founded ~44 kya, while extant distributions along western and eastern Atlantic shores were founded at the end of the Last Glacial Maximum (~19 kya), with at least one major refuge being the North Carolina region. The recent colonization and five- to sevenfold lower genomic diversity of the Atlantic compared to the Pacific populations raises concern and opportunity about how Atlantic eelgrass might respond to rapidly warming coastal oceans.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2020

ExINP-ENA Ice-Nucleating Particle Concentrations - CRAFT

This data set comprises cumulative ambient ice-nucleating particle (INP) concentrations measured at the U.S. Department of Energy's Atmospheric Radiation Measurement (ARM) Eastern North Atlantic (ENA) observatory on Graciosa Island, Azores (39.0916° N, 28.0257° W). Our INP data were generated using a combination of online instruments, the Portable Ice Nucleation Experiment chamber (PINE), and an offline assay instrument, the West Texas Cryogenic Refrigerator Applied to Freezing Test system (WT-CRAFT), for aerosol particle samples collected on polycarbonate filters (with 0.2-micron diameter pores).

47-mm Nuclepore filter sampler, West Texas Cryogen↗

DOE OSTI2020

ExINP-ENA Ice-Nucleating Particle Concentrations - PINE

54 ENVIRONMENTAL SCIENCES↗

DOE OSTI2025

A family of tubular pili from harmful algal bloom forming cyanobacterium Microcystis aeruginosa

Cyanobacteria are vital photosynthetic prokaryotes, but some form harmful algal blooms (cyanoHABs) that disrupt ecosystems and produce toxins. The mechanisms by which these blooms form have yet to be fully understood, particularly the role of extracellular components. Here, we present a 2.4 Å cryo-EM structure of a pilus, termed the cyanobacterial tubular (CT) pilus, found in the cyanoHAB-forming Microcystis aeruginosa. The pilin exhibits a unique protein fold, forming a tubular pilus structure with tight, double-layer anti-parallel β-sheet interactions. We show that CT pili are essential for buoyancy by facilitating the formation of micro-colonies, which increases drag force and prevents sinking. The CT pilus surface is heavily glycosylated with ten monosaccharide modifications per pilin. Furthermore, CT pili can enrich microcystin, potentially enhancing cellular resilience, and co-localize with iron-enriched extracellular matrix components. Thus, we propose that this pilus plays an important role in the proliferation of cyanoHABs. This just discovered pilus family appears to be widely distributed across several cyanobacterial orders. Our structural and functional characterization of CT pili provide insights into cyanobacterial cell morphology, physiology, and toxin interactions, and identify potential targets for disrupting bloom formation.

Cryoelectron microscopy↗