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At least 145 records · Page 8

Survival and Age at Maturity in Head-Started Wood Turtles ( Glyptemys insculpta ) with Implications for Population Recovery

A small relict population of Glyptemys insculpta (Wood Turtle) was discovered on a protected area in New Jersey in 2006. Marking and radio-tracking of the old founder individuals helped to determine movement patterns and habitat use. Monitoring of nesting females revealed that nesting habitat and nest success was limited due to invasive plants, human landscape alteration, and Procyon lotor (Raccoon) depredation. We initiated habitat restoration including creation of protected nesting areas, mowing in winter, invasive plant removal, and adjacent landowner education. Here, we direct-released hatchlings from protected nests from 2006 to 2015, yet only a few were detected in subsequent years. Between 2011 and 2023, some or all of each hatchling cohort were head-started indoors at a high school for 9 months. We have continuously radio-tracked all head-starts from the 2011 cohort and portions of the 2012–2014 cohorts. Head-started turtles found their own food, established home ranges, and hibernated communally with founder adults. Subsidized Raccoons, mowers, automobiles, and flooding events—all human-instigated—were the causes of mortality. The first males and first females from the 2011 head-start cohort reached maturity in 2017 and 2019, respectively, at ages 6–8, younger than expected by 4–5 years. Successful reproduction by head-starts was confirmed by viable hatchlings produced in 2019, 2020, and 2023. Head-starting can pull a relict population out of the nose-dive to extirpation when used in conjunction with habitat-restoration practices, but it must be conducted with persistence and continuity over at least the number of years it takes for the earliest cohorts to reach maturity and begin producing offspring of their own.

59 BASIC BIOLOGICAL SCIENCES↗

Wood-to-Fuel for California's Transportation Sector using Autothermal Pyrolysis, March 2020 (Progress Report for FRD-17-002)

In the month of March, we planned to have the Site License Agreement with SPI signed and fully executed, make further progress on the market assessment for the Wood-to-Bio-oil Market Report, and hoped to have the subcontracts in place with Iowa State University and Frontline BioEnergy, LLC. We also hoped to have the updated budget from Iowa State in order to send both updated sub-recipient budget sheets to the CAM for approval.

09 BIOMASS FUELS↗

Wood-to-Fuel for California's Transportation Sector using Autothermal Pyrolysis (Progress Report for FRD-17-002)

In the month of April, we planned to have the Site License Agreement with SPI signed and fully executed, make further progress on the market assessment for the Wood-to-Bio-oil Market Report, and hoped to have the subcontracts in place with Iowa State University and Frontline BioEnergy, LLC once we have approval from the CAM. We also plan to have the updated budget from Iowa State in order to send both updated sub-recipient budget sheets to the CAM for approval.We continue to make more progress towards getting our subcontracts in place and received the requested adjustments to ISU’s budget sheets. These were sent to the CAM on 4/30/2020. As the COVID-19 situation is country-wide, we anticipate slower responses from our partners due to similar delays in their organizations to what we are experiencing internally. Although their response times will be slower, both ISU and Frontline are continuing to make good progress on their work for the demonstration in Iowa for this summer, which is assisting with keeping us on schedule for the overall effort. We are continuing to work on the required documents for our subcontracts – though neither will be placed before we receive official approval from our CAM – so that once we have approval, we can place them very quickly.

09 BIOMASS FUELS↗

Wood-to-Fuel for California's Transportation Sector using Autothermal Pyrolysis (Progress Report)

In the month of May, we planned to have the Site License Agreement with SPI signed and fully executed, make further progress on the market assessment for the Wood-to-Bio-oil Market Report, and hoped to have the subcontracts in place with Iowa State University and Frontline BioEnergy, LLC once we ha approval from the CAM. We also planned to have the updated Advanced Reconciliation to the CAM for approval as requested.

09 BIOMASS FUELS↗

Experimental investigation on the binary/ternary fluidization behavior of Geldart D type spherical LDPE, Geldart D type cylindrical wood and Geldart B type sand particles

The series of experiments have been performed on a 2.5-inch cylindrical bed made of acrylic with full optical access and multiple pressure ports at various heights of the bed. The material of interest includes in the investigation include Geldart type D cylindrical hardwood pellets, Geldart type D spherical LDPE, and Geldart type B sand particles. The wood particles have a diameter of around 6.6 mm and a length of 13.2 mm, corresponding to an aspect ratio of 1:2. The spherical LDPE particles and the sand particles have a Sauter Mean Diameter of 3.75 mm, and 0.5 mm, respectively. The experiment series involved the fluidization of the above-mentioned single particle type, as well as the fluidization of binary and ternary mixtures with varying superficial velocities.

20 FOSSIL-FUELED POWER PLANTS↗

Wood-to-Fuel for California's Transportation Sector using Autothermal Pyrolysis (Progress Report for FRD-17-002)

Accomplishments this period: Subcontract with Frontline BioEnergy, LLC has been executed and is in place (Task 1). List of TAC members is awaiting input from the CEC including any additional members of their own choosing before we finalize confirmation with the listed individuals. This is also holding off on the documented TAC Member commitment, and the DRAFT TAC Meeting Schedule, as we need to know who is approved to be on the TAC before we get commitments and start setting meetings. We are awaiting input from the CEC as to a requested date for the CPR#1. It was originally set for the beginning of July (July 7th), but as the CEC needs to conduct this, we need their input on the revised date. The additional wood feed stock was sent to ISU for analysis of autothermal pyrolysis products on July 15. The Kern refinery continues to express interest in using the bio-oil produced from the demonstration in one or more of their plants. We believe this analysis information will be a critical component of what we share at our first Workshop later this summer

09 BIOMASS FUELS↗

Single-Cell Genomics of Novel Actinobacteria With the Wood–Ljungdahl Pathway Discovered in a Serpentinizing System

Serpentinite-hosted systems represent modern-day analogs of early Earth environments. In these systems, water-rock interactions generate highly alkaline and reducing fluids that can contain hydrogen, methane, and low-molecular-weight hydrocarbons-potent reductants capable of fueling microbial metabolism. In this study, we investigated the microbiota of Hakuba Happo hot springs (~50°C; pH~10.5–11), located in Nagano (Japan), which are impacted by the serpentinization process. Analysis of the 16S rRNA gene amplicon sequences revealed that the bacterial community comprises Nitrospirae (47%), “Parcubacteria” (19%), Deinococcus-Thermus (16%), and Actinobacteria (9%), among others. Notably, only 57 amplicon sequence variants (ASV) were detected, and fifteen of these accounted for 90% of the amplicons. Among the abundant ASVs, an early-branching, uncultivated actinobacterial clade identified as RBG-16-55-12 in the SILVA database was detected. Ten single-cell genomes (average pairwise nucleotide identity: 0.98–1.00; estimated completeness: 33–93%; estimated genome size: ~2.3 Mb) that affiliated with this clade were obtained. Taxonomic classification using single copy genes indicates that the genomes belong to the actinobacterial class-level clade UBA1414 in the Genome Taxonomy Database. Based on metabolic pathway predictions, these actinobacteria are anaerobes, capable of glycolysis, dissimilatory nitrate reduction and CO 2 fixation via the Wood–Ljungdahl (WL) pathway. Several other genomes within UBA1414 and two related class-level clades also encode the WL pathway, which has not yet been reported for the Actinobacteria phylum. For the Hakuba actinobacterium, the energy metabolism related to the WL pathway is likely supported by a combination of the Rnf complex, group 3b and 3d [NiFe]-hydrogenases, [FeFe]-hydrogenases, and V-type (H + /Na + pump) ATPase. The genomes also harbor a form IV ribulose 1,5-bisphosphate carboxylase/oxygenase (RubisCO) complex, also known as a RubisCO-like protein, and contain signatures of interactions with viruses, including clustered regularly interspaced short palindromic repeat (CRISPR) regions and several phage integrases. This is the first report and detailed genome analysis of a bacterium within the Actinobacteria phylum capable of utilizing the WL pathway. The Hakuba actinobacterium is a member of the clade UBA1414/RBG-16-55-12, formerly within the group “OPB41.” We propose to name this bacterium ‘Candidatus Hakubanella thermoalkaliphilus.'

59 BASIC BIOLOGICAL SCIENCES↗

Experimental Studies on Wood Pellets Combustion in a Fixed Bed Combustor Using Taguchi Method

The combustion of wood pellets in a fixed bed combustor of a 20 kW capacity domestic pellet boiler was tested according to several factors including Power, Excess Air (EA), Primary/Secondary air Split Ratio (SR) and Grate Area (GA). The Taguchi method was applied to program the experimental design. Several parameters were measured, including gas emissions (CO), fuel bed temperature (measured at 4 different heights), and efficiency. The experimental results show that the lower CO emission and the higher efficiency were obtained at medium thermal loads and the highest temperature on the fuel bed was obtained at about ¼ of its height (15 mm). The results obtained from the analysis of variance (ANOVA) show that the SR and the Power are the most important factors contributing to the CO reduction and also increase the fuel bed temperature.

Castro, Carlos↗

Effects on the Sustainable Development Goals of Wood Pellet Production in the Southeastern United States

Wood-based pellets produced in the southeastern United States (SE US) and shipped overseas for the generation of heat and power support achievement of several of the United Nation’s Sustainable Development Goals and their targets. If improperly implemented, pellet supply chains, like most energy technologies, can have negative impacts on water and air quality, biodiversity, and other ecosystem services. Cooperation between regional and local industry partners and local communities as well as readily accessible information on environmental and societal effects of the industry are important for addressing sustainability goals. Strengths of this supply chain include replacement of fossil coal with bioenergy; support for renewable energy goals; conservation of forests through sustainable, green economy jobs; and more efficient use of waste materials.

09 BIOMASS FUELS↗

Truncating the distribution of modulus properties in natural populations of wood

This disclosure is directed to methods of separating wood veneer material by property measurements, and thereafter improving the properties of veneer material with property values lower than a target threshold value, until those materials meet or exceed that threshold values. In some aspects of the disclosure, veneer materials are prepared, non-destructively measured, and separated into passing and failing material collections. The failing material collection may then be treated to improve the density and flexural modulus of the material therein. Also disclosed herein are products and materials incorporating the treated veneer materials according to the disclosed methods.

Bradshaw, Allan↗

Soil fertility and drought interact to determine large variations in wood production for a hyperdominant Amazonian tree species

The productivity of the Amazon Rainforest is related to climate and soil fertility. However, the degrees to which these interactions influence multiannual to decadal variations in tree diameter growth are still poorly explored. To fill this gap, we used radiocarbon measurements to evaluate the variation in tree growth rates over the past decades in an important hyperdominant species, Eschweilera coriacea (Lecythidaceae), from six sites in the Brazilian Amazon that span a range of soil properties and climate. Using linear mixed-effects models, we show that temporal variations in mean annual diameter increment evaluated over a specific time period reflect interactions between soil fertility and the drought index (SPEI-Standardized Precipitation and Evapotranspiration Index). Our results indicate that the growth response of trees to drought is strongly dependent on soil conditions, a facet of forest productivity that is still underexplored, and which has great potential for improving predictions of future tropical tree growth in the face of projected climate change.

54 ENVIRONMENTAL SCIENCES↗

Genetically engineered poplar wood effectively enhances the efficiency of deep eutectic solvent-mediated one-pot processing

Although lignocellulosic biomass is a renewable resource with the potential to replace fossil-derived fuels and chemicals, its recalcitrance, largely due to lignin, limits its utilization. Recent advancements in genetic engineering have produced transgenic trees with reduced lignin content and/or modified lignin structure without compromising growth traits. Here, three engineered poplar varieties are evaluated as feedstocks using a biocompatible one-pot deep eutectic solvent-mediated process that integrates biomass fractionation and enzymatic saccharification within a single reactor, eliminating water washing and reconditioning. All transgenic poplars exhibit higher fermentable sugar yields than wild-type (WT) trees. Notably, QsuB poplar, incorporating 3,4-dihydroxybenzoate in lignin, achieves the highest glucose conversion yield of 91.3% (vs. 73.0% from WT). AT5 and MdCHS3 poplars, incorporating ferulate esters and naringenin, also demonstrate improved glucose yields (86.7 and 84.7%, respectively), confirming reduced biomass recalcitrance. Additionally, residual lignins are valorized via hydrogenolysis into phenolic compounds, with comparable alkylphenol production across all lines. These findings demonstrate that the transgenic poplar lines not only serve as superior feedstocks for sugar conversion but also provide a rich resource for phenolic compound production, enhancing the operational and economic viability of integrated biorefinery processes.

biomasses↗