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At least 145 records · Page 8

Isolation and characterization of IgG3 glycan-targeting antibodies with exceptional cross-reactivity for diverse viral families

Broadly reactive antibodies that target sequence-diverse antigens are of interest for vaccine design and monoclonal antibody therapeutic development because they can protect against multiple strains of a virus and provide a barrier to evolution of escape mutants. Using LIBRA-seq (linking B cell receptor to antigen specificity through sequencing) data for the B cell repertoire of an individual chronically infected with human immunodeficiency virus type 1 (HIV-1), we identified a lineage of IgG3 antibodies predicted to bind to HIV-1 Envelope (Env) and influenza A Hemagglutinin (HA). Two lineage members, antibodies 2526 and 546, were confirmed to bind to a large panel of diverse antigens, including several strains of HIV-1 Env, influenza HA, coronavirus (CoV) spike, hepatitis C virus (HCV) E protein, Nipah virus (NiV) F protein, and Langya virus (LayV) F protein. We found that both antibodies bind to complex glycans on the antigenic surfaces. Antibody 2526 targets the stem region of influenza HA and the N-terminal domain (NTD) region of SARS-CoV-2 spike. A crystal structure of 2526 Fab bound to mannose revealed the presence of a glycan-binding pocket on the light chain. Antibody 2526 cross-reacted with antigens from multiple pathogens and displayed no signs of autoreactivity. These features distinguish antibody 2526 from previously described glycan-reactive antibodies. Further study of this antibody class may aid in the selection and engineering of broadly reactive antibody therapeutics and can inform the development of effective vaccines with exceptional breadth of pathogen coverage.

Microbiology

Care 3 phase 2 report, maintenance manual

CARE 3 (Computer-Aided Reliability Estimation, version three) is a computer program designed to help estimate the reliability of complex, redundant systems. Although the program can model a wide variety of redundant structures, it was developed specifically for fault-tolerant avionics systems--systems distinguished by the need for extremely reliable performance since a system failure could well result in the loss of human life. It substantially generalizes the class of redundant configurations that could be accommodated, and includes a coverage model to determine the various coverage probabilities as a function of the applicable fault recovery mechanisms (detection delay, diagnostic scheduling interval, isolation and recovery delay, etc.). CARE 3 further generalizes the class of system structures that can be modeled and greatly expands the coverage model to take into account such effects as intermittent and transient faults, latent faults, error propagation, etc.

Bryant, L. A.

The CARE 3 Phase 3 Report: Test and Evaluation

CARE 3 (Computer-Aided Reliability Estimation, version three) is a computer program designed to help estimate the reliability of complex, redundant systems; although the program can model a wide variety of redundant structures, it was developed specifically for fault-tolerant avionics systems, systems distinguished by the need for extremely reliable performance since a system failure could well result in the loss of human life. CARE 3 further generalizes the class of system structures that can be modeled and greatly expands the coverage model to take into account such effects as intermittent and transient faults, latent faults, error propagation, etc. The initial test and evaluation of CARE 3 are reported.

Stiffler, J. J.

CARE 3 phase 2 report - mathematical description

CARE III (Computer-Aided Reliability Estimation, version three) a computer program designed to help estimate the reliability of complex, redundant systems is described. Although the program can model a wide variety of redundant structures, it was developed specifically for fault tolerant avionics systems. CARE III generalizes the class of system structures that can be modeled and greatly expands the coverage model to take into account such effects as intermittent and transient faults, latent faults, and error propagation.

Stiffler, J. J.

Investigation of low energy space plasma

A statistical study of 1982 data for occurrences of equatorially trapped plasma has been extended. The previous survey, which utilized only the MSFC summary fiche, has been supplemented with the GSFC summary fiche, which has had the effect of substantially improving the late 1982 coverage. It was found that in the post midnight region (1 - 3 LT), the trapped plasma is limited to + or - 5 degrees magnetic latitude, while in the early afternoon (13-15 LT), latitude ranges as high as + or 30 degrees are found. This survey has provided a link to earlier ATS-6 and ISEE studies of pancake distributions. Although the most energetic, and most anisotopic plasmas are trapped within a few degrees of the equator, the results of these equatorial interactions extend substantially along the magnetic field line in the afternoon and dusk region and these high latitude extensions were previously studied by the Huntsville group. Results of this study were incorporated into a revision of the equatorial ion paper, which has been resubmitted and accepted for publication.

Comfort, R. H.

Market opportunities in Canada for multimedia residential services in rural and small urban areas

This paper reviews the studies which were undertaken jointly by Telesat and Industry Canada to provide an estimate of the market opportunities for residential multi-media services in the rural and small urban areas of Canada. This study is part of the Advanced Satcom program, a Ka-band satellite system proposal which is currently in the implementation proposal phase by the government and the Canadian space industry of which Telesat is an active member. Advanced Satcom extends the reach of terrestrial information highways to the remote and sparsely populated parts of the country in a cost-effective manner and thus provides a ubiquitous coverage of the information highways to all Canadians. Therefore, the rural and small urban markets are believed to be good opportunities for the Advanced Satcom. Although the results are primarily intended for fixed residential applications, they can also be used as input to market opportunity studies for wideband mobile applications.

Shariatmadar, Mehran

Negative Electron Affinity Mechanism for Diamond Surfaces

The energy distribution of the secondary electrons for chemical vacuum deposited diamond films with Negative Electron Affinity (NEA) was investigated. It was found that while for completely hydrogenated diamond surfaces the negative electron affinity peak in the energy spectrum of the secondary electrons is present for any energy of the primary electrons, for partially hydrogenated diamond surfaces there is a critical energy above which the peak is present in the spectrum. This critical energy increases sharply when hydrogen coverage of the diamond surface diminishes. This effect was explained by the change of the NEA from the true type for the completely hydrogenated surface to the effective type for the partially hydrogenated surfaces.

Krainsky, I. L.

Spectral Gradients and u-v Sampling

I describe a systematic method for characterizing the effects of differences in u-v coverage in terms of inferred spectral gradients in interferometric images. This method is directly applicable to optical and radio interferometry; it is useful in situations when observations using scale arrays are not feasible.

u-v sampling spectral gradients interferometric im

Advanced X-Ray Timing Array Mission: Conceptual Spacecraft Design Study

The Advanced X-Ray Timing Array (AXTAR) is a mission concept for submillisecond timing of bright galactic x-ray sources. The two science instruments are the Large Area Timing Array (LATA) (a collimated instrument with 2-50-keV coverage and over 3 square meters of effective area) and a Sky Monitor (SM), which acts as a trigger for pointed observations of x-ray transients. The spacecraft conceptual design team developed two spacecraft concepts that will enable the AXTAR mission: A minimal configuration to be launched on a Taurus II and a larger configuration to be launched on a Falcon 9 or similar vehicle.

Hopkins, R. C.

Retrieval of Stratospheric Aerosol Size Distribution Parameters Using Satellite Solar Occultation Measurements at Three Wavelengths

In this work, a novel approach for the determination of the particle size distribution (PSD) parameters of stratospheric sulfate aerosols is presented. For this, ratios of extinction coefficients obtained from SAGE III/ISS (Stratospheric Aerosol and Gas Experiment III on the International Space Station) solar occultation measurements at 449, 756 and 1544 nm were used to retrieve the mode width and median radius of a size distribution assumed to be monomodal lognormal. The estimated errors at the peak of the stratospheric aerosol layer, on average, lie between 20% and 25% for the median radius and 5% and 7% for the mode width. The results are consistent in magnitude with other retrieval results from the literature, but a robust comparison is difficult, mainly because of differences in temporal and spatial coverage. Other quantities like number density and effective radius were also calculated. A major advantage of the described method over other retrieval techniques is that both the median radius and the mode width can be retrieved simultaneously, without having to assume one of them. This is possible due to the broad wavelength spectrum covered by the SAGE III/ISS measurements. Also, the presented method – being based on the analysis of three wavelengths – allows unique solutions for the retrieval of PSD parameters for almost all of the observed extinction spectra, which is not the case when using only two spectral channels. In addition, the extinction coefficients from SAGE III/ISS solar occultation measurements, on which the retrieval is based, are calculated without a priori assumptions about the PSD. For those reasons, the data produced with the presented retrieval technique may be a valuable contribution for a better understanding of the variability of stratospheric aerosol size distributions, e.g. after volcanic eruptions. While this study focuses on describing the retrieval method, and a future study will discuss the PSD parameter data set produced in depth, some exemplary results for background conditions in June 2017 are shown.

Felix Wrana

The Analysis of Using Satellite Soil Moisture Observations for Flood Detection, Evaluating Over the Thailand’s Great Flood of 2011

A flood monitoring and warning system provides critical information that can protect property and save lives. A basin-scale flood monitoring system requires an effective observation platform that offers extensive ground coverage of flood conditions, low latency, and high spatiotemporal resolution. While satellite imagery offers substantial spatial flood extent in detail due to its high spatial resolution, the coarse temporal resolution and cloud obstruction limit its near real-time application. Daily soil moisture data derived from satellite sensors at a scale of a few km can be used to monitor extreme wet surface conditions arising in flood occurrences. This study analyses the flood detection capabilities of several sources of soil moisture information, including the Soil Moisture and Ocean Salinity mission (SMOS), the Advanced Microwave Scanning Radiometer on EOS (AMSR-E), the Advanced SCATterometer (ASCAT) on MetOp, the Global Land Data Assimilation System (GLDAS), and the WaterGAP Global Hydrology Model (WGHM). In addition to soil moisture, the analysis includes measurements of surface reflectance from the Moderate Resolution Imaging Spectroradiometer (MODIS), precipitation measurements from the Tropical Rainfall Measuring Mission (TRMM), and terrestrial water storage estimates from the Gravity Recovery And Climate Experiment (GRACE) as proxies for flood inundations. The analysis was conducted over the Chao Phraya River Basin (CPB) in Thailand, where the Great Flood of 2011 led to one of the most significant economic losses in the country's history. Satellite-derived soil moisture exhibits a stronger correlation with the flood inundations than the precipitation, model-derived soil moisture, and terrestrial water storage data. SMOS soil moisture observation agrees best with the MODIS-derived flood extent/occurrence, both in terms of spatial distribution and timing, and providing approximated flood lead-time of one week or longer. A neural network constructed from SMOS and MODIS data is used to predict flood intensity/occurrence (given soil moisture input) with a predicted time window from eight days to thirty-two days. The short-term prediction (e.g., eight days) achieves the highest accuracy with an averaged recovery rate of approximately 60% (correlation coefficient). This study's results suggest a potential application of satellite soil moisture data in assisting flood monitoring and warning systems.

Satellite soil moisture

A Comparison of Stratospheric Smoke Events Seen by SAGE II (1984-2005) and SAGE III (2017-2021)

The ongoing Stratospheric Aerosol and Gas Experiment aboard the International Space Station (SAGE III/ISS) has mostly occurred during a period of relatively low aerosol loading. At the same time, it has almost observed a number of small to moderate injections of volcanic material in the stratosphere from the Aoba/Ambae eruption of 2018 to that of Karymsky in 2022. In addition, two of the largest known smoke intrusions into the stratosphere have occurred during the SAGE III/ISS mission (the British Columbia fire of August 2017 and the Australian Wildfire Event of 2019/2020). At least two other much smaller smoke events have also been observed in the SAGE III data (California Creek in September 2020 and McKay Creek in June 2021). Particularly with the two large events, it is tempting to speculate that these events are increasing in intensity and frequency possibly due to ongoing climate change. Realistically, change is difficult to assess since instrument data sets that can be used for quantifying historical events can be quite different in what and where they provide measurements. This complicates comparing one event to another potentially separated by many years. An exception to this issues are the measurements by SAGE III/ISS and those of its predecessor SAGE II (1984-2005). Both use the same measurement strategy, measure aerosol extinction coefficient at 1020 nm, and have similar spatial/temporal coverage. Both instruments are known to observe smoke events in the stratosphere. However, both have the same low observation density characteristic of solar occultation such that near-global coverage takes almost a month and characterizing effectively instant events like smoke and volcanic events take a matter of weeks depending on the vagaries of where the observations are being made at a critical juncture unlike instruments with high temporal/spatial resolution like limb-scatter instruments such as OMPS and OSIRIS. The goal of this work will be to use the same analysis approach to both the SAGE II and SAGE III data and compare both the frequency and intensity of smoke events observed by these instruments. While we do not expect to be able to make conclusions about change between the periods, the analysis is a first step in interpreting changes in the contribution of smoke to the stratospheric aerosol budget between the time frames encompassed by these instruments.

L. W. Thomason

Marangoni Effects of a Drop in an Extensional Flow: The Role of Surfactant Physical Chemistry

While the changes in stresses caused by surfactant adsorption on non-deforming interfaces have been fairly well established, prior to this work, there were few studies addressing how surfactants alter stresses on strongly deforming interfaces. We chose the model problem of a drop in a uniaxial extensional flow to study these stress conditions To model surfactant effects at fluid interfaces, a proper description of the dependence of the surface tension on surface concentration, the surface equation of state, is required. We have adopted a surface equation of state that accounts for the maximum coverage limit; that is, because surfactants have a finite cross sectional area, there is an upper bound to the amount of surfactant that can adsorb in a monolayer. The surface tension reduces strongly only when this maximum coverage is approached. Since the Marangoni stresses go as the derivative of the surface equation of state times the surface concentration gradient, the non-linear equation of state determines both the effect of surfactants in the normal stress jump, (which is balanced by the product of the mean curvature of the interface times the surface tension), and the tangential stress jump, which is balanced by Marangoni stresses. First, the effects of surface coverage and intermolecular interactions among surfactants which drive aggregation of surfactants in the interface were studied. (see Pawar and Stebe, Physics of Fluids).

Stebe, Kathleen J.

LANDSAT-4 World Reference System (WRS) users guide

A functional description of the new LANDSAT-4 World Reference System (WRS) with an overview of the main orbital parameters and instrument coverages is presented to provide the data user with the primary information required to understand LANDSAT-4 orbital characteristics, to effectively use the WRS indexing scheme, and to request specific geographic coverage on the desired observation dates.

Source record

Hydrogen Adsorption over Transition Metals in Water

The adsorption free energy of atomic hydrogen on Pt(111), Pd(111), Ni(111), Ru(0001), Cu(111), and Rh(111) in liquid water was computed using a quantum mechanical/molecular mechanical free-energy perturbation scheme. The Pt(111) computations indicate that the solvent effect on H adsorption on atop sites (+0.20 eV) is almost twice that on fcc (+0.12 eV), showing it is less likely to find adsorbed hydrogen in atop position in the presence of water than in the gas phase. The solvent effect for the fcc site, which is the most favorable site for adsorbed H on Pt(111), agrees qualitatively with experimental work by Lercher et al., who reported an effect of +0.2 eV. Overall, an endergonic solvent effect for hydrogen adsorption is observed for all metals, indicating a lower hydrogen coverage relative to free site coverage at metal-water interfaces compared to metal-gas interfaces, even when hydrogen transport effects through the fluid phase are negligible; a result with important implications for (de)hydrogenation catalysis.

Zare, Mehdi

LANDSAT-D Worldwide Reference System (WRS) users guide

A functional description of the LANDSAT-D Worldwide Reference System (WRS) and an overview of the main orbital parameters and instrument coverages are presented. The primary information required to understand the LANDSAT-D orbital characteristics, to effectively use the Worldwide Reference System (WRS) indexing scheme, and to request specific geographic coverage on the desired observation dates is provided.

Source record

Gravity model improvement using the DORIS tracking system on the SPOT 2 satellite

A high-precision radiometric satellite tracking system, Doppler Orbitography and Radio-positioning Integrated by Satellite system (DORIS), has recently been developed by the French space agency, Centre National d'Etudes Spatiales (CNES). DORIS was designed to provide tracking support for missions such as the joint United States/French TOPEX/Poseidon. As part of the flight testing process, a DORIS package was flown on the French SPOT 2 satellite. A substantial quantity of geodetic quality tracking data was obtained on SPOT 2 from an extensive international DORIS tracking network. These data were analyzed to assess their accuracy and to evaluate the gravitational modeling enhancements provided by these data in combination with the Goddard Earth Model-T3 (GEM-T3) gravitational model. These observations have noise levels of 0.4 to 0.5 mm/s, with few residual systematic effects. Although the SPOT 2 satellite experiences high atmospheric drag forces, the precision and global coverage of the DORIS tracking data have enabled more extensive orbit parameterization to mitigate these effects. As a result, the SPOT 2 orbital errors have been reduced to an estimated radial accuracy in the 10-20 cm RMS range. The addition of these data, which encompass many regions heretofore lacking in precision satellite tracking, has significantly improved GEM-T3 and allowed greatly improved orbit accuracies for Sun-synchronous satellites like SPOT 2 (such as ERS 1 and EOS). Comparison of the ensuing gravity model with other contemporary fields (GRIM-4C2, TEG2B, and OSU91A) provides a means to assess the current state of knowledge of the Earth's gravity field. Thus, the DORIS experiment on SPOT 2 has provided a strong basis for evaluating this new orbit tracking technology and has demonstrated the important contribution of the DORIS network to the success of the TOPEX/Poseidon mission.

Nerem, R. S.

Visual field information in Nap-of-the-Earth flight by teleoperated Helmet-Mounted displays

The human ability to derive Control-Oriented Visual Field Information from teleoperated Helmet-Mounted displays in Nap-of-the-Earth flight, is investigated. The visual field with these types of displays originates from a Forward Looking Infrared Radiation Camera, gimbal-mounted at the front of the aircraft and slaved to the pilot's line-of-sight, to obtain wide-angle visual coverage. Although these displays are proved to be effective in Apache and Cobra helicopter night operations, they demand very high pilot proficiency and work load. Experimental work presented in the paper has shown that part of the difficulties encountered in vehicular control by means of these displays can be attributed to the narrow viewing aperture and head/camera slaving system phase lags. Both these shortcomings will impair visuo-vestibular coordination, when voluntary head rotation is present. This might result in errors in estimating the Control-Oriented Visual Field Information vital in vehicular control, such as the vehicle yaw rate or the anticipated flight path, or might even lead to visuo-vestibular conflicts (motion sickness). Since, under these conditions, the pilot will tend to minimize head rotation, the full wide-angle coverage of the Helmet-Mounted Display, provided by the line-of-sight slaving system, is not always fully utilized.

Grunwald, Arthur J.