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At least 145 records · Page 8

New method for estimating bacterial cell abundances in natural samples by use of sublimation

We have developed a new method based on the sublimation of adenine from Escherichia coli to estimate bacterial cell counts in natural samples. To demonstrate this technique, several types of natural samples, including beach sand, seawater, deep-sea sediment, and two soil samples from the Atacama Desert, were heated to a temperature of 500 degrees C for several seconds under reduced pressure. The sublimate was collected on a cold finger, and the amount of adenine released from the samples was then determined by high-performance liquid chromatography with UV absorbance detection. Based on the total amount of adenine recovered from DNA and RNA in these samples, we estimated bacterial cell counts ranging from approximately 10(5) to 10(9) E. coli cell equivalents per gram. For most of these samples, the sublimation-based cell counts were in agreement with total bacterial counts obtained by traditional DAPI (4,6-diamidino-2-phenylindole) staining.

Evaluation Studies↗

An engineering method for estimating notch-size effect in fatigue tests on steel

Neuber's proposed method of calculating a practical factor of stress concentration for parts containing notches of arbitrary size depends on the knowledge of a "new material constant" which can be established only indirectly. In this paper, the new constant has been evaluated for a large variety of steels from fatigue tests reported in the literature, attention being confined to stresses near the endurance limit. Reasonably satisfactory results were obtained with the assumption that the constant depends only on the tensile strength of the steel. Even in cases where the notches were cracks of which only the depth was known, reasonably satisfactory agreement was found between calculated and experimental factors. It is also shown that the material constant can be used in an empirical formula to estimate the size effect on unnotched specimens tested in bending fatigue.

Kuhn, Paul↗

Weight and cost forecasting for advanced manned space vehicles

A mass and cost estimating computerized methology for predicting advanced manned space vehicle weights and costs was developed. The user friendly methology designated MERCER (Mass Estimating Relationship/Cost Estimating Relationship) organizes the predictive process according to major vehicle subsystem levels. Design, development, test, evaluation, and flight hardware cost forecasting is treated by the study. This methodology consists of a complete set of mass estimating relationships (MERs) which serve as the control components for the model and cost estimating relationships (CERs) which use MER output as input. To develop this model, numerous MER and CER studies were surveyed and modified where required. Additionally, relationships were regressed from raw data to accommodate the methology. The models and formulations which estimated the cost of historical vehicles to within 20 percent of the actual cost were selected. The result of the research, along with components of the MERCER Program, are reported. On the basis of the analysis, the following conclusions were established: (1) The cost of a spacecraft is best estimated by summing the cost of individual subsystems; (2) No one cost equation can be used for forecasting the cost of all spacecraft; (3) Spacecraft cost is highly correlated with its mass; (4) No study surveyed contained sufficient formulations to autonomously forecast the cost and weight of the entire advanced manned vehicle spacecraft program; (5) No user friendly program was found that linked MERs with CERs to produce spacecraft cost; and (6) The group accumulation weight estimation method (summing the estimated weights of the various subsystems) proved to be a useful method for finding total weight and cost of a spacecraft.

Williams, Raymond↗

Modeling Global Water Use for the 21st Century: Water Futures and Solutions (WFaS) Initiative and Its Approaches

To sustain growing food demand and increasing standard of living, global water use increased by nearly 6 times during the last 100 years, and continues to grow. As water demands get closer and closer to the water availability in many regions, each drop of water becomes increasingly valuable and water must be managed more efficiently and intensively. However, soaring water use worsens water scarcity conditions already prevalent in semi-arid and arid regions, increasing uncertainty for sustainable food production and economic development. Planning for future development and investments requires that we prepare water projections for the future. However, estimations are complicated because the future of the world's waters will be influenced by a combination of environmental, social, economic, and political factors, and there is only limited knowledge and data available about freshwater resources and how they are being used. The Water Futures and Solutions (WFaS) initiative coordinates its work with other ongoing scenario efforts for the sake of establishing a consistent set of new global water scenarios based on the shared socio-economic pathways (SSPs) and the representative concentration pathways (RCPs). The WFaS "fast track" assessment uses three global water models, namely H08, PCR-GLOBWB, and WaterGAP. This study assesses the state of the art for estimating and projecting water use regionally and globally in a consistent manner. It provides an overview of different approaches, the uncertainty, strengths and weaknesses of the various estimation methods, types of management and policy decisions for which the current estimation methods are useful. We also discuss additional information most needed to be able to improve water use estimates and be able to assess a greater range of management options across the water-energy-climate nexus.

soaring↗

Method to Estimate the Dissolved Air Content in Hydraulic Fluid

In order to verify the air content in hydraulic fluid, an instrument was needed to measure the dissolved air content before the fluid was loaded into the system. The instrument also needed to measure the dissolved air content in situ and in real time during the de-aeration process. The current methods used to measure the dissolved air content require the fluid to be drawn from the hydraulic system, and additional offline laboratory processing time is involved. During laboratory processing, there is a potential for contamination to occur, especially when subsaturated fluid is to be analyzed. A new method measures the amount of dissolved air in hydraulic fluid through the use of a dissolved oxygen meter. The device measures the dissolved air content through an in situ, real-time process that requires no additional offline laboratory processing time. The method utilizes an instrument that measures the partial pressure of oxygen in the hydraulic fluid. By using a standardized calculation procedure that relates the oxygen partial pressure to the volume of dissolved air in solution, the dissolved air content is estimated. The technique employs luminescent quenching technology to determine the partial pressure of oxygen in the hydraulic fluid. An estimated Henry s law coefficient for oxygen and nitrogen in hydraulic fluid is calculated using a standard method to estimate the solubility of gases in lubricants. The amount of dissolved oxygen in the hydraulic fluid is estimated using the Henry s solubility coefficient and the measured partial pressure of oxygen in solution. The amount of dissolved nitrogen that is in solution is estimated by assuming that the ratio of dissolved nitrogen to dissolved oxygen is equal to the ratio of the gas solubility of nitrogen to oxygen at atmospheric pressure and temperature. The technique was performed at atmospheric pressure and room temperature. The technique could be theoretically carried out at higher pressures and elevated temperatures.

Hauser, Daniel M.↗

Effect of sampling rate and record length on the determination of stability and control derivatives

Flight data from five aircraft were used to assess the effects of sampling rate and record length reductions on estimates of stability and control derivatives produced by a maximum likelihood estimation method. Derivatives could be extracted from flight data with the maximum likelihood estimation method even if there were considerable reductions in sampling rate and/or record length. Small amplitude pulse maneuvers showed greater degradation of the derivative maneuvers than large amplitude pulse maneuvers when these reductions were made. Reducing the sampling rate was found to be more desirable than reducing the record length as a method of lessening the total computation time required without greatly degrading the quantity of the estimates.

Brenner, M. J.↗

Effects of time-shifted data on flight determined stability and control derivatives

Flight data were shifted in time by various increments to assess the effects of time shifts on estimates of stability and control derivatives produced by a maximum likelihood estimation method. Derivatives could be extracted from flight data with the maximum likelihood estimation method even if there was a considerable time shift in the data. Time shifts degraded the estimates of the derivatives, but the degradation was in a consistent rather than a random pattern. Time shifts in the control variables caused the most degradation, and the lateral-directional rotary derivatives were affected the most by time shifts in any variable.

Steers, S. T.↗

Methods for estimating pressure and thermal loads induced by elevon deflections on hypersonic-vehicle surfaces with turbulent boundary layers

Empirical anaytic methods are presented for calculating thermal and pressure distributions in three-dimensional, shock-wave turbulent-boundary-layer, interaction-flow regions on the surface of controllable hypersonic aircraft and missiles. The methods, based on several experimental investigations, are useful and reliable for estimating both the extent and magnitude of the increased thermal and pressure loads on the vehicle surfaces.

Kaufman, L. G., II↗

Relative effectiveness of several simulated jet engine noise spectral treatments in reducing annoyance in a TV-viewing situation

An experiment was conducted in order to determine the relative effectiveness of several hypothetical jet engine noise treatments and to test hypothesis that speech interference, at least in part, mediates annoyance in a TV-viewing situation. Twenty-four subjects watched television in a simulated living room. Recorded jet flyover noises were presented in such a way as to create the illusion that aircraft were actually flying overhead. There were 27 stimuli (nine spectra at three overall levels) presented at an average rate of approximately one flight every 2 minutes. Subjects judged the annoyance value of individual stimuli using either a category rating method or magnitude estimation method in each of two 1-hour sessions. The spectral treatments most effective in reducing annoyance were at 1.6 Khz and 800 Hz, in that order. The degree of annoyance reduction resulting from all treatments was affected by the overall sound level of the stimuli, with the greatest reduction at the intermediate overall sound level, about 88 to 89 db(A), peak value. The results are interpreted as supporting the hypothesis that speech interference, at least in part, mediates annoyance with aircraft noise in a TV-viewing situation.

Gunn, W. J.↗

An efficient method for estimating neighboring steady-state numerical solutions to the Euler equations

The paper concentrates on a linear approximation method for predicting the changes occurring in steady-state numerical solutions of the Euler equations as a consequence of small changes in the independent variables which control the problem. The importance of proper boundary-condition treatment and other issues concerning the problem are covered along with the importance of proper algorithm selection for a fully supersonic inviscid flow. The method is applied to a subsonic nozzle involving variation of the pressure on the outflow boundary and to a supersonic inlet involving variation of the inflow Mach number. In the subsonic test case, the comparisons between the predicted and conventional numerical solutions are shown to be good, while in the supersonic test case, the agreement between the approximation method and conventional numerical solution starts out well but rapidly degenerates at some point in the flowfield as the perturbation of the boundary conditions is increased.

Taylor, Arthur C., III↗

A simple method for estimating minimum autorotative descent rate of single rotor helicopters

Flight test results of minimum autorotative descent rate are compared with calculations based on the minimum power required for steady level flight. Empirical correction factors are derived that account for differences in energy dissipation between these two flight conditions. A method is also presented for estimating the minimum power coefficient for level flight for any helicopter for use in the empirical estimation procedure of autorotative descent rate.

Talbot, P. D.↗

Inverse Method for Estimation of Composite Kink-Band Toughness from Open-Hole Compression Strength Data

Fiber-reinforced polymer matrix composite materials can fail by kink-band propagation mechanism when subjected to in-plane compressive loading. This mode of failure is especially prevalent in compressive loading of laminates with holes, cut-outs, or impact damage. Most of the successful models for predicting compressive strength of such laminates require “fracture” toughness associated with kink-band propagation under in-plane compression. However, this property is difficult to measure experimentally, limiting the use of such models in design practice. In this paper an inverse method is proposed to estimate the kink-band toughness of the laminate from its open-hole compression strength data, which is an easier property to measure experimentally. Furthermore, a scaling relationship is proposed to estimate kink-band toughness for other laminate configurations of the same material.

Luke Borkowski↗

An activity-based methodology for operations cost analysis

This report describes an activity-based cost estimation method, proposed for the Space Exploration Initiative (SEI), as an alternative to NASA's traditional mass-based cost estimation method. A case study demonstrates how the activity-based cost estimation technique can be used to identify the operations that have a significant impact on costs over the life cycle of the SEI. The case study yielded an operations cost of $101 billion for the 20-year span of the lunar surface operations for the Option 5a program architecture. In addition, the results indicated that the support and training costs for the missions were the greatest contributors to the annual cost estimates. A cost-sensitivity analysis of the cultural and architectural drivers determined that the length of training and the amount of support associated with the ground support personnel for mission activities are the most significant cost contributors.

Korsmeyer, David↗

A Method of Estimating Residuals in Orbital Theory

The degree of approximation used in determining the orbits of earth satellites is reflected in the residuals (differences between calculated and observed positions). The least-squares procedure generally used to fit theory to observation tends to obscure the significance of theoretical parameters, so that the physical sources of residuals cease to be apparent. A method is outlined herein for estimating the magnitude of the residuals to be expected from an approximate theory presumed to have one missing or incorrect term.

Lecar, Myron↗

A Novel Method for Estimating Shortwave Direct Radiative Effect of Above-Cloud Aerosols Using CALIOP and MODIS Data

This paper describes an efficient and unique method for computing the shortwave direct radiative effect (DRE) of aerosol residing above low-level liquid-phase clouds using CALIOP and MODIS data. It addresses the overlap of aerosol and cloud rigorously by utilizing the joint histogram of cloud optical depth and cloud top pressure while also accounting for subgrid-scale variations of aerosols. The method is computationally efficient because of its use of grid-level cloud and aerosol statistics, instead of pixel-level products, and a pre-computed look-up table based on radiative transfer calculations. We verify that for smoke over the southeast Atlantic Ocean the method yields a seasonal mean instantaneous (approximately 1:30PM local time) shortwave DRE of above cloud aerosol (ACA) that generally agrees with more rigorous pixel-level computation within 4 percent. We also estimate the impact of potential CALIOP aerosol optical depth (AOD) retrieval bias of ACA on DRE. We find that the regional and seasonal mean instantaneous DRE of ACA over southeast Atlantic Ocean would increase, from the original value of 6.4 W m(-2) based on operational CALIOP AOD to 9.6 W m(-2) if CALIOP AOD retrieval are biased low by a factor of 1.5 (Meyer et al., 2013) and further to 30.9 W m(-2) if CALIOP AOD retrieval are biased low by a factor of 5 as suggested in (Jethva et al., 2014). In contrast, the instantaneous ACA radiative forcing efficiency (RFE) remains relatively invariant in all cases at about 53 W m(-2) AOD(-1), suggesting a near linear relation between the instantaneous RFE and AOD. We also compute the annual mean instantaneous shortwave DRE of light-absorbing aerosols (i.e., smoke and polluted dust) over global oceans based on 4 years of CALIOP and MODIS data. We find that the variability of the annual mean shortwave DRE of above-cloud light-absorbing aerosol is mainly driven by the optical depth of the underlying clouds. While we demonstrate our method using CALIOP and MODIS data, it can also be extended to other satellite data sets, as well as climate model outputs.

CALIOP and MODIS↗

A rapid method of estimating the collision frequencies between the earth and the earth-crossing bodies

The present study examines a very fast method of calculating the collision frequency between two low-eccentricity orbiting bodies for evaluating the evolution of earth-orbiting objects such as space debris. The results are very accurate and the required computer time is negligible. The method is now applied without modification to calculate the collision frequencies for moderately and highly eccentric orbits.

Su, Shin-Yi↗