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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 145 records · Page 8
All sourcesNASA NTRSDOE OSTI
NASA NTRS1990

Discovery of molecular hydrogen fluorescence in the diffuse interstellar medium

The first detection of molecular hydrogen fluorescence in the diffuse interstellar medium is reported. Using the Berkeley UVX Shuttle Spectrometer, H2 Lyman band fluorescence has been observed in four directions, each with high significance. Molecular hydrogen fluorescence is detected in all directions that have previously been found to contain significant CO emission. A simple equilibrium model has been developed that includes attenuation of the incident UV radiation field by H2 line and dust continuum absorption. Evidence is found that the gas in the CO emission portions of the clouds may be clumpy, with a filling factor less than 0.2 and an average density greater than 30/cu cm in most cases.

Martin, Christopher↗
DOE OSTI2026

INL CRMO XRF Special Report No. 3: INL CRMO XRF Calibration Methods for the Analysis of Ceramics and Brick

This report details the analytical methods used by the Idaho National Laboratory Cultural Resource Management Office to characterize ceramic and brick artifacts using X-ray fluorescence spectrometry. More specifically, this report provides details on instrumentation, analysis times, and matrix-specific calibration protocols for ceramic materials. Calibration curves were developed to adjust raw instrumental output for 13 elements using a suite of reference standards made from historic brick borrowed from the Yale Peabody Museum. Calibration curves were validated by plotting predicted values against published reference values for the calibration set as well as a certified soil reference standard (SRM 2711a).

36 MATERIALS SCIENCE↗
DOE OSTI2025

Volumetric imaging of the 3D orientation of cellular structures with a polarized fluorescence light-sheet microscope

Polarized fluorescence microscopy is a valuable tool for measuring molecular orientations in biological samples, but techniques for recovering three-dimensional orientations and positions of fluorescent ensembles are limited. We report a polarized dual-view light-sheet system for determining the diffraction-limited three-dimensional distribution of the orientations and positions of ensembles of fluorescent dipoles that label biological structures. We share a set of visualization, histogram, and profiling tools for interpreting these positions and orientations. We model the distributions based on the polarization-dependent efficiency of excitation and detection of emitted fluorescence, using coarse-grained representations we call orientation distribution functions (ODFs). We apply ODFs to create physics-informed models of image formation with spatio-angular point-spread and transfer functions. We use theory and experiment to conclude that light-sheet tilting is a necessary part of our design for recovering all three-dimensional orientations. We use our system to extend known two-dimensional results to three dimensions in FM1-43-labeled giant unilamellar vesicles, fast-scarlet-labeled cellulose in xylem cells, and phalloidin-labeled actin in U2OS cells. Additionally, we observe phalloidin-labeled actin in mouse fibroblasts grown on grids of labeled nanowires and identify correlations between local actin alignment and global cell-scale orientation, indicating cellular coordination across length scales.

Science & Technology - Other Topics↗
NASA NTRS2018

Reduced Solar-Induced Chlorophyll Fluorescence from GOME-2 During Amazon Drought Caused by Dataset Artifacts

Recently, Yang et al. (2018) reported a decrease in solar‐induced chlorophyll fluorescence (SIF) during 2015/2016 El Nino event albeit the increase in enhanced vegetation index (EVI). They interpreted the reduced SIF as a signal of reduced ecosystem photosynthesis. However, we argue that the reduced SIF during 2015/2016 is caused by a decreasing trend of SIF due to sensor degradation and the satellite overpass time is critical for drought impact assessment.

Solar‐induced chlorophyll fluorescence (SIF) and↗
NASA NTRS1993

In situ detection of tropospheric OH, HO2, NO2, and NO by laser-induced fluorescence in detection chambers at reduced pressures

This report is a brief summary of the status of work on the grant entitled 'In situ detection of tropospheric OH, HO2, NO2, and NO by laser induced fluorescence in detection chambers at low pressures.' The basic instrument characteristics have been established, and have been reported in a manuscript, included as an appendix to this report, that has been accepted by the Journal of Geophysical Research. Currently, two efforts are under way. First, instrument tests and calibrations are continuing. These efforts include field measurements and an informal inter comparison in Colorado last August and September. Second, new technologies in lasers and detectors are being implemented to make the instrument smaller, lighter, and more energy efficient. Such instrument modifications are essential for measurements from aircraft, high scaffolding in forests, and ships.

Brune, William H.↗
DOE OSTI2022

Laser-induced-fluorescence imaging of a spin-polarized ultracold neutral plasma in a magnetic field

In this work, we report Doppler-sensitive laser-induced-fluorescence (LIF) imaging of an ultracold neutral plasma in a magnetic field. Local values of ion density, hydrodynamic fluid velocity, temperature, and spin polarization are obtained using a fluorescence model based on velocity-resolved rate equations (REs) including the transfer of ions between states due to laser coupling and spontaneous emission. The RE approach captures optical pumping of ions into states that are not driven by the LIF excitation laser, and this is validated with experimental data. Combined molecular-dynamics and quantum-trajectories simulations verify that velocity-changing collisions have a negligible impact on the state population evolution for typical experimental conditions. Relative intensities of Zeeman components of the LIF spectra provide clear evidence that the ions are electron-spin-polarized when created by photoionization of magnetically trapped 88 Sr atoms. This probe opens many possibilities for studying thermal transport and the equilibration of neutral plasmas in overlapping regimes of strong coupling and magnetization.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗
DOE OSTI2020

Comparison of total emitted solar-induced chlorophyll fluorescence (SIF) and top-of-canopy (TOC) SIF in estimating photosynthesis

We report many studies have shown that solar-induced fluorescence (SIF) has a good potential to predict gross primary production (GPP) of vegetation. What we measured by remote sensing or near-surface platforms is top-of-canopy (TOC) SIF (SIF toc ), which is not necessarily equal or proportional to total emitted SIF (SIF tot ) from the entire canopy due to the (re)absorption and scattering effects. However, photosynthesis, the process that plants use to fix carbon from the atmosphere, occurs at the entire vertical canopy. Here, by using the recollision theory, we calculated SIF tot at 760 nm from the measured SIF toc , hyperspectral reflectance (R), canopy interception (i) and leaf albedo (ω l ). Among them, both SIF toc and R can be obtained from concurrent TOC spectral measurements; i and ω l in the near-infrared region can be estimated from the open access datasets with a good accuracy. Our result confirms that the measured SIF toc only accounts for a small fraction of SIF tot : the above-the-canopy sensor can only “see” on average 22.9% of SIF tot at Harvard Forest. SIF tot has the following advantages over SIF toc in estimating GPP: (1) SIF tot improves the diurnal estimate of canopy GPP, especially capable to capture the midday depression of photosynthesis which may cause the large discrepancies between SIF toc and GPP on a diurnal basis, (2) SIF tot produces a stronger correlation with GPP from plants with complex canopy structure or under sky conditions with more diffuse irradiance, and (3) the SIF tot -GPP relationship shows a stronger resilience to environmental stresses. The fluorescence escape ratio (f esc ), the ratio between SIF toc multiplied by π and SIF tot , is mostly determined by the sun-canopy-sensor geometry and leaf inclination distribution. The effect of LAI and the leaf chlorophyll concentration on f esc is marginal at the 760 nm wavelength. Our results suggest that converting SIF toc into SIF tot provides a better tool to understand and estimate GPP.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2020

Time-resolved quantum beats in the fluorescence of helium resonantly excited by XUV radiation

Abstract We report on the observation of time-resolved quantum beats in the helium fluorescence from the transition 1 s 3 p → 1 s 2 s , where the initial state is excited by XUV free electron laser radiation. The quantum beats originate from the Zeeman splitting of the magnetic substates due to an external magnetic field. We perform a systematic study of this effect and discuss the possibilities of studying this phenomenon in the XUV and x-ray regime.

Optics↗
NASA NTRS1992

In situ detection of tropospheric OH, HO2, NO2, and NO by laser-induced fluorescence in detection chambers at reduced pressures

This report is a brief summary of the status of work on the grant entitled 'In situ detection of tropospheric OH, HO2, NO2, and NO by laser induced fluorescence in detection chambers at low pressures'. The first version of the instrument is essentially complete and operational for about six months, and we continue to make improvements on the instrument sensitivity and reliability. We are focusing our efforts on improving our understanding of the operating characteristics of the instrument - particularly the inlet transmission for OH and HO2, the exact character of the air flow around and within the instrument, and the efficiency of the chemical conversion of HO2 to OH. We are also in the process of converting this laboratory instrument into a field worthy instrument that we can take to remote sites for measurements.

Brune, William H.↗
DOE OSTI2021

Spectroscopic and computational investigations of organometallic complexation of group 12 transition metals by methanobactins from Methylocystis sp. SB2

Methanotrophic bacteria catalyze the aerobic oxidation of methane to methanol using Cu-containing enzymes, thereby exerting a modulating influence on the global methane cycle. To facilitate the acquisition of Cu ions, some methanotrophic bacteria secrete small modified peptides known as “methanobactins,” which strongly bind Cu and function as an extracellular Cu recruitment relay, analogous to siderophores and Fe. In addition to Cu, methanobactins form complexes with other late transition metals, including the Group 12 transition metals Zn, Cd, and Hg, although the interplay among solution-phase configurations, metal interactions, and the spectroscopic signatures of methanobactin-metal complexes remains ambiguous. In this study, the complexation of Zn, Cd, and Hg by methanobactin from Methylocystis sp. strain SB2 was studied using a combination of absorbance, fluorescence, extended x-ray absorption fine structure (EXAFS) spectroscopy, and time-dependent density functional theory (TD-DFT) calculations. We report changes in sample absorbance and fluorescence spectral dynamics, which occur on a wide range of experimental timescales and characterize a clear stoichiometric complexation dependence. Mercury L3-edge EXAFS and TD-DFT calculations suggest a linear model for Hg--S coordination, and TD-DFT suggests a tetrahedral model for Zn 2+ and Cd 2+ . We observed an enhancement in the fluorescence of methanobactin upon interaction with transition metals and propose a mechanism of complexation-hindered isomerization drawing inspiration from the wild-type Green Fluorescent Protein active site. Collectively, our results represent the first combined computational and experimental spectroscopy study of methanobactins and shed new light on molecular interactions and dynamics that characterize complexes of methanobactins with Group 12 transition metals.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2021

Striking luminescence phenomena of carbon dots and their applications as a double ratiometric fluorescence probes for H2S detection

Here, we report some new observations on the luminescence of carbon dots (CDs) that are passivated with polyethylene glycol, and explored them, for the first time, as highly sensitive H2S detection probe. The as-prepared CDs have an ultraviolet (UV) emission at 350 nm and a green emission at 540 nm when excited at 270 nm, but they have only a green emission at 523 nm when excited at 365 nm. As Na2S is added to the CDs, the UV emission decreases gradually, while the green emission increases slightly, and interestingly a new emission appears at 455 nm is increased linearly in intensity with the increase of Na2S concentration. The blue emission at 455 nm is from the intrinsic core of CDs, and the green emissions at 540 or 523 nm are from their surface states, while the UV emission at 350 nm is from the anchored PEG. Both the emission intensity ratios of F-450/F-350 and F-455/F-523 show an excellent linear relationship with the Na2S concentration in the range of 0-800 mu M, and the detection limit estimated from both of them is approximately 7.0 nM, indicating our method is the most sensitive H2S CDs based detection method reported thus far. Furthermore, the detection was tested successfully for H2S imaging in live cells. We propose that the interactions of H2S with the CDs and the anchored ligands, as well as the energy transfer between the ligands and the CDs, are responsible for the luminescence responses for H2S detection. Our studies enrich the world of carbon dots with plenty of potential applications. (C) 2020 Elsevier Ltd. All rights reserved.

Bioimaging↗
DOE OSTI2026

Development of high throughput light-sheet fluorescence lifetime imaging microscopy for 3D functional imaging of metabolic pathways in plant and microorganisms (Final Technical Report)

This research program will enable new biochemical contrast in the nanosecond lifetime domain through use of the recently demonstrated electro-optic fluorescence lifetime imaging technique (EO-FLIM) for wide-field lifetime imaging. The Stanford/Stanford Linear Accelerator Center multidisciplinary collaboration -- physics, applied physics, and structural biology -- will develop a light-sheet fluorescence lifetime imaging microscope for functional studies of microbial and plant metabolic pathways and dynamic interactions between plants and microorganisms in the rhizosphere. The proposed approach overcomes the imaging time bottleneck associated with existing fluorescence lifetime imaging methods. Initial demonstrations have shown a factor of 100,000 improvement in photon throughput compared to existing methods. High photon efficiency allowed the first wide-field fluorescence lifetime imaging of single molecules. Recent work has improved the technique’s repetition rate to enable compatibility with mode-locked lasers and demonstrated the combination of wide-field fluorescence lifetime imaging with super-resolution localization microscopy, observations of single molecule dynamics, and observation of donor lifetime quenching in single-molecule imaging. These results were achieved on standard camera sensors and would not have been possible with other wide-field approaches. The throughput and photon economy of the EO-FLIM method enables new BER-relevant imaging opportunities. In particular, scanned single- and two-photon light-sheet excitation will be used to achieve volumetric imaging with time-domain contrast.

47 OTHER INSTRUMENTATION↗
DOE OSTI2020

Electrochemical Switching of a Fluorescent Molecular Rotor Embedded within a Bistable Rotaxane

We report how the nanoconfined environment, introduced by the mechanical bonds within an electrochemically switchable bistable [2]rotaxane, controls the rotation of a fluorescent molecular rotor, namely, an 8-phenyl-substituted boron dipyrromethene (BODIPY). The electrochemical switching of the bistable [2]rotaxane induces changes in the ground-state coconformation and in the corresponding excited-state properties of the BODIPY rotor. In the starting redox state, when no external potential is applied, the cyclobis(paraquat-p-phenylene) (CBPQT4+) ring component encircles the tetrathiafulvalene (TTF) unit on the dumbbell component, leaving the BODIPY rotor unhindered and exhibiting low fluorescence. Upon oxidation of the TTF unit to a TTF2+ dication, the CBPQT4+ ring is forced toward the molecular rotor, leading to an increased energy barrier for the excited state to rotate the rotor into the state with a high nonradiative rate constant, resulting in an overall 3.4-fold fluorescence enhancement. On the other hand, when the solvent polarity is high enough to stabilize the excited charge-transfer state between the BODIPY rotor and the CBPQT4+ ring, movement of the ring toward the BODIPY rotor produces an unexpectedly strong fluorescence signal decrease as the result of photoinduced electron transfer from the BODIPY rotor to the CBPQT4+ ring. The nanoconfinement effect introduced by mechanical bonding can effectively lead to modulation of the physicochemical properties as observed in this bistable [2]rotaxane. On account of the straightforward synthetic strategy and the facile modulation of switchable electrochromic behavior, our approach could pave the way for the development of new stimuli-responsive materials based on mechanically interlocked molecules for future electro-optical applications, such as sensors, molecular memories, and molecular logic gates.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
NASA NTRS1973

Detection of S02 and N02 in Stack Plume by Raman Scattering and Fluorescence

We have been studying laser-Raman radar which can be used as the remote detector of sulfur dioxide concentration in the stack plume of boiler exhaust gas, and some results have been published. In this paper, we report the interference of nitrogen dioxide fluorescence against sulfur dioxide Raman scattering and the measuring method of sulfur dioxide and nitrogen dioxide concentration. In a stack plume, high density dust and high concentration carbon dioxide are included, therefore very strong Mie back-scattering and carbon dioxide Raman scattering are observed. The separation of these scattering signals from sulfur dioxide Raman signal was the first problem for the laser-Raman radar. But, this problem was solved by using the filter which have high resolving power. It is well known that nitrogen dioxide can be excited to emit fluorescence of broad spectrum by blue-green light. The light source of the laser-Raman radar is SH of Nd:YAG laser, and boiler exhaust gas includes several tens ppm of nitrogen dioxide, then the interference of nitrogen dioxide fluorescence brings the error to sulfur dioxide measurement. 'rhe rejection of the interference can be achieved by the subtraction of the nitrogen dioxide fluorescence contribution from detected signal at sulfur dioxide Raman scattering wavelength. The nitrogen dioxide fluorescence contribution can be measured by two methods. The first is to convert the nitrogen dioxide fluorescence intensity which is measured at a different wavelength from sulfur dioxide Raman line into the one at sulfur dioxide Raman line. The second is to convert the detected signal intensity, which is obtained when the range gate is set just behind the plume, into the one obtained at the range of the plume using the difference of the time dependence between sulfur dioxide Raman scattering and nitrogen dioxide fluorescence. Nitrogen dioxide fluorescence has lifetime of about 300nS, while Raman scattering has none. By either of the two methods, the contribution of nitrogen dioxide fluorescence is determined. Then, we can measure the nitrogen dioxide and sulfur dioxide concentration.The minimum detectable concentration and the experimental results of the remote sensing of sulfur dioxide and nitrogen dioxide in stack plume will be discussed in detail.

Nakahara, S.↗
NASA NTRS1985

A two-photon laser-induced fluorescence field instrument for ground-based and airborne measurements of atmospheric NO

This paper reports on a new two-photon laser-induced fluorescence (TP-LIF) sensor capable of making routine measurements at the few parts per trillion volume level. This direct spectroscopic detection method has been demonstrated to be a reliable instrument while performing both on the ground and in the air. As currently designed it is unique in being 'signal' rather than 'signal-to-noise' limited. The latter characteristic enables the TP-LIF sensor to make atmospheric measurements of NO under environmental conditions that might normally be considered unsuitable for a laser technique. These include clouds, rain, and, in general, high-atmospheric-aerosol loading conditions. Of special interest is the insensitivity of the TP-LIF NO instrument to changes in pressure while operating in the troposphere. This characteristic has enabled this sensor to be used to record real-time altitude profiles of NO. Future improvements should make possible two measurement opportunities: (1) NO flux measurements via the airborne eddy-correlation method and (2) nitrogen isotopic distribution measurements (e.g., (N-15)(0-16) versus (N-14)(0-16) as a means of identifying specific NO(x) sources.

Bradshaw, J. D.↗
DOE OSTI2022

A new μ -high energy resolution fluorescence detection microprobe imaging spectrometer at the Stanford Synchrotron Radiation Lightsource beamline 6-2

In this report we describe a new synchrotron X-ray Fluorescence (XRF) imaging instrument with an integrated High Energy Fluorescence Detection X-ray Absorption Spectroscopy (HERFD-XAS) spectrometer at the Stanford Synchrotron Radiation Lightsource at beamline 6-2. The X-ray beam size on the sample can be defined via a range of pinhole apertures or focusing optics. XRF imaging is performed using a continuous rapid scan system with sample stages covering a travel range of 250 × 200 mm 2 , allowing for multiple samples and/or large samples to be mounted. The HERFD spectrometer is a Johann-type with seven spherically bent 100 mm diameter crystals arranged on intersecting Rowland circles of 1 m diameter with a total solid angle of about 0.44% of 4π sr. A wide range of emission lines can be studied with the available Bragg angle range of ~64.5°–82.6°. With this instrument, elements in a sample can be rapidly mapped via XRF and then selected features targeted for HERFD-XAS analysis. Furthermore, utilizing the higher spectral resolution of HERFD for XRF imaging provides better separation of interfering emission lines, and it can be used to select a much narrower emission bandwidth, resulting in increased image contrast for imaging specific element species, i.e., sparse excitation energy XAS imaging. This combination of features and characteristics provides a highly adaptable and valuable tool in the study of a wide range of materials.

47 OTHER INSTRUMENTATION↗
DOE OSTI2022

Porous Polymers as Universal Reversal Agents for Heparin Anticoagulants through an Inclusion–Sequestration Mechanism

Heparins are widely used anticoagulants for surgical procedures and extracorporeal therapies. However, all of them have bleeding risks. Protamine sulfate, the only clinically approved antidote for unfractionated heparin (UFH), has adverse effects. Moreover, protamine can only partially neutralize low-molecular-weight heparins (LMWHs) and is not effective for fondaparinux. In this report an inclusion–sequestration strategy for efficient neutralization of heparin anticoagulants by cationic porous supramolecular organic frameworks (SOFs) and porous organic polymers (POPs) is reported. Isothermal titration calorimetric and fluorescence experiments show strong binding affinities of these porous polymers toward heparins, whereas dynamic light scattering and zeta potential analysis confirm that the heparin sequences are adsorbed into the interior of the porous hosts. Activated partial thromboplastin time, anti-FXa, and thromboelastography assays indicate that their neutralization efficacies are higher than or as high as that of protamine for UFH and generally superior to protamine for LMWHs and fondaparinux, which is further confirmed by tail-transection model in mice and ex vivo aPTT or anti-FXa analysis in rats. Acute toxicity evaluations reveal that one of the SOFs displays outstanding biocompatibility. This work suggests that porous polymers can supply safe and rapid reversal of clinically used heparins, as protamine surrogates, providing an improved approach for their neutralization.

36 MATERIALS SCIENCE↗
DOE OSTI2020

Measurements of pressure-induced $Kβ$ line shifts in ramp compressed cobalt up to 8 Mbar

Here we report measurements of K -shell fluorescence lines induced by fast electrons in ramp-compressed Co targets. The fluorescence emission was stimulated by fast electrons generated through short-pulse laser-solid interaction with an Al target layer. Compression up to 2.1 × solid density was achieved while maintaining temperatures well below the Fermi energy, effectively removing the thermal effects from consideration. We observed small but unambiguous redshifts in the K β fluorescence line relative to unshifted Cu K α . Redshifts up to 2.6 eV were found to increase with compression and to be consistent with predictions from self-consistent models based on density-functional theory.

75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND↗