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At least 145 records · Page 8

Multigene engineering in plants: Technologies, applications, and future prospects

The emerging bioeconomy presents a promising solution to both economic and environmental challenges. Within the bioeconomy, plants serve as a renewable, sustainable, and cost-effective source of foods, fuels, chemicals, and materials. However, traditional breeding and single-gene engineering approaches fall short in addressing complex traits (e.g., drought tolerance, disease resistance, yield, nutrient use efficiency) which are controlled by multiple genes. The complexity of plant biology often necessitates the use of multigene engineering (MGE), which involves simultaneous ectopic expression, up/down-regulation, or editing of multiple genes, to enhance plant traits relevant to the bioeconomy. These genes may be associated with distinct traits or function as components of specific metabolic and regulatory pathways. This review summarizes current technologies for MGE within the synthetic biology-driven Design-Build-Test-Learn (DBTL) framework, detailing its four key stages: Design – gene construct development; Build – DNA assembly and plant transformation; Test – the molecular, biochemical, and physiological characterization of engineered plants; and Learn – computational modeling to refine, multiplex and iterate the process. Despite good progress in the applications of MGE in biofortification, metabolic engineering, and stress resilience, challenges remain in construct stability, coordinated gene expression, and regulatory predictability. We identified optimization paths and future directions to accelerate MGE deployment in sustainable agriculture, with possible societal benefits including reduced production costs, increased yield, and improved food and nutritional security.

AI-aided plant engineering↗

Historical and Future Global Irrigation Energy Consumption by Fuel and Region

Irrigation energy use is a significant component of agricultural production costs, contributing directly to the energy and emissions intensity of crop production and ultimately to food prices. Understanding the existing structure of irrigation energy consumption help achieve food-energy-water security and environmental goals. We present a comprehensive global data set detailing country-level irrigation energy consumption, emphasizing the comparative use of electric, diesel, and emerging solar pumps. To our knowledge, no such data set exists. We draw from a literature review to develop a logistic transformed regression model to estimate the shares of fuel sources for irrigation across countries over historical years to construct a global data set of country-level irrigation energy consumption by multiple fuel sources. Additionally, we compare our estimates of irrigation energy use with agricultural energy use as reported by the International Energy Agency and other external sources. We then use this data to project future irrigation energy use with the Global Change Analysis Model, which is a multisector dynamics model, to showcase the usage of this data set. Projections under the reference scenario show a global shift in fuel types for irrigation pumping, while patterns vary across regions, with India and Pakistan leading in solar-powered irrigation growth and countries like the USA and China continuing to rely primarily on grid electricity. This data set provides a resource to understand the role of irrigation fuel choices within the broader energy sector, as well as the connected agricultural, land use, and water sectors under alternative future scenarios, enabling informed decision making toward efficient agricultural practices.

Global Change Analysis Model (GCAM)↗

Chromosome evolution and the genetic basis of agronomically important traits in greater yam

The nutrient-rich tubers of the greater yam, Dioscorea alata L., provide food and income security for millions of people around the world. Despite its global importance, however, greater yam remains an orphan crop. Here, we address this resource gap by presenting a highly contiguous chromosome-scale genome assembly of D. alata combined with a dense genetic map derived from African breeding populations. The genome sequence reveals an ancient allotetraploidization in the Dioscorea lineage, followed by extensive genome-wide reorganization. Using the genomic tools, we find quantitative trait loci for resistance to anthracnose, a damaging fungal pathogen of yam, and several tuber quality traits. Genomic analysis of breeding lines reveals both extensive inbreeding as well as regions of extensive heterozygosity that may represent interspecific introgression during domestication. These tools and insights will enable yam breeders to unlock the potential of this staple crop and take full advantage of its adaptability to varied environments.

59 BASIC BIOLOGICAL SCIENCES↗

Genomic mechanisms of climate adaptation in polyploid bioenergy switchgrass

Long-term climate change and periodic environmental extremes threaten food and fuel security and global crop productivity. Although molecular and adaptive breeding strategies can buffer the effects of climatic stress and improve crop resilience, these approaches require sufficient knowledge of the genes that underlie productivity and adaptation—knowledge that has been limited to a small number of well-studied model systems. Here we present the assembly and annotation of the large and complex genome of the polyploid bioenergy crop switchgrass ( Panicum virgatum ). Analysis of biomass and survival among 732 resequenced genotypes, which were grown across 10 common gardens that span 1,800 km of latitude, jointly revealed extensive genomic evidence of climate adaptation. Climate–gene–biomass associations were abundant but varied considerably among deeply diverged gene pools. Furthermore, we found that gene flow accelerated climate adaptation during the postglacial colonization of northern habitats through introgression of alleles from a pre-adapted northern gene pool. The polyploid nature of switchgrass also enhanced adaptive potential through the fractionation of gene function, as there was an increased level of heritable genetic diversity on the nondominant subgenome. In addition to investigating patterns of climate adaptation, the genome resources and gene–trait associations developed here provide breeders with the necessary tools to increase switchgrass yield for the sustainable production of bioenergy.

09 BIOMASS FUELS↗

Unique contributions of chlorophyll and nitrogen to predict crop photosynthetic capacity from leaf spectroscopy

The photosynthetic capacity or the CO 2 -saturated photosynthetic rate (Vmax), chlorophyll, and nitrogen are closely linked leaf traits that determine C 4 crop photosynthesis and yield. Accurate, timely, rapid, and non-destructive approaches to predict leaf photosynthetic traits from hyperspectral reflectance are urgently needed for high-throughput crop monitoring to ensure food and bioenergy security. Therefore, this study thoroughly evaluated the state-of-the-art physically based radiative transfer models (RTMs), data-driven partial least squares regression (PLSR), and generalized PLSR (gPLSR) models to estimate leaf traits from leaf-clip hyperspectral reflectance, which was collected from maize (Zea mays L.) bioenergy plots with diverse genotypes, growth stages, treatments with nitrogen fertilizers, and ozone stresses in three growing seasons. The results show that leaf RTMs considering bidirectional effects can give accurate estimates of chlorophyll content (Pearson correlation r=0.95), while gPLSR enabled retrieval of leaf nitrogen concentration (r=0.85). Using PLSR with field measurements for training, the cross-validation indicates that V max can be well predicted from spectra (r=0.81). Here, the integration of chlorophyll content (strongly related to visible spectra) and nitrogen concentration (linked to shortwave infrared signals) can provide better predictions of V max (r=0.71) than only using either chlorophyll or nitrogen individually. This study highlights that leaf chlorophyll content and nitrogen concentration have key and unique contributions to V max prediction.

59 BASIC BIOLOGICAL SCIENCES↗

Data from Unique Contributions of Chlorophyll and Nitrogen to Predict Crop Photosynthetic Capacity from Leaf Spectroscopy

The photosynthetic capacity or the CO2-saturated photosynthetic rate (Vmax), chlorophyll, and nitrogen are closely linked leaf traits that determine C4 crop photosynthesis and yield. Accurate, timely, rapid, and non-destructive approaches to predict leaf photosynthetic traits from hyperspectral reflectance are urgently needed for high-throughput crop monitoring to ensure food and bioenergy security. Therefore, this study thoroughly evaluated the state-of-the-art physically based radiative transfer models (RTMs), data-driven partial least squares regression (PLSR), and generalized PLSR (gPLSR) models to estimate leaf traits from leaf-clip hyperspectral reflectance, which was collected from maize (Zea mays L.) bioenergy plots with diverse genotypes, growth stages, treatments with nitrogen fertilizers, and ozone stresses in three growing seasons. The results show that leaf RTMs considering bidirectional effects can give accurate estimates of chlorophyll content (Pearson correlation r=0.95), while gPLSR enabled retrieval of leaf nitrogen concentration (r=0.85). Using PLSR with field measurements for training, the cross-validation indicates that Vmax can be well predicted from spectra (r=0.81). The integration of chlorophyll content (strongly related to visible spectra) and nitrogen concentration (linked to shortwave infrared signals) can provide better predictions of Vmax (r=0.71) than only using either chlorophyll or nitrogen individually. This study highlights that leaf chlorophyll content and nitrogen concentration have key and unique contributions to Vmax prediction.

Biomass Analytics↗

Influence of Vegetation Canopy Architecture on Dynamics of Spatiotemporal Light Distribution and its Effect on Land-Biosphere-Atmosphere Fluxes of Energy, Carbon, and Water

The dynamic spatiotemporal distribution of light and micro climatic variables (wind speed, atmospheric CO 2 concentration, humidity, pressure, and air temperature) within the plant canopy as determined by the canopy architecture play a critical role in land-biosphere-atmosphere (LBA) photosynthetic carbon uptake, transpiratory water loss and energy partitioning. Vegetation models that include effects of sun and shade flecks, leaf temperature and energy balance partitioning between sensible and latent heat fluxes, and reduced turbulent mixing in dense vegetation canopies can accurately predict canopy performance, particularly its shaded leaves, which can constitute 80% of leaf area and contribute up to 50% of carbon update and 65% of water loss. However, current vegetation models with their bulk characterization of shaded leaves poorly represent the complex three dimensional (3D) canopy architecture are insufficient to capture the spatiotemporal dynamics of shaded leaves. Here, we propose to employ an x-ray computed tomography (XCT) imaging technique in combination with an automated, high throughput, image processing pipeline (3DArch, to be developed) to obtain an explicit 3D triangulated surface mesh of a plant canopy (Fig 1). 3DArch would provide a much needed technological jump in 3D LBA modeling where computer generated artificial 3D models suffer from lack of high resolution detailed canopy architecture parameters. The 3D canopy surface mesh output from 3DArch is coupled with an explicit 3D forward ray tracing model (fastTracer) to obtain a high resolution representation of the dynamic spatiotemporal light environment within the canopy over the course of a day (Fig 1). The results from the light attenuation model are then coupled with a process based multi-scale bio-physical canopy and leaf model of photosynthesis (PlantGro) to get fluxes of carbon, water and energy at the leaf and canopy scale, in addition to estimating whole plant carbon uptake, and water loss. Our proposed model framework would be the first of its kind to provide a breakthrough capability in the field of process-based plant modeling that bridges the key technological gap in shaded leaf modeling through explicit representation of 3D canopy architecture. Computer generated artificial 3D model canopy simulations on grasses show that the canopy light distribution does not follow an exponential behavior as assumed in most 1D vegetation models, but rather exhibits a heterogeneous behavior, with a small but significant portion of interior canopy leaves experiencing peak light levels due to sun flecks while the rest of the canopy receives substantially lower light levels. These results indicate a bias in traditional models that overestimates shaded leaf contribution towards whole canopy photosynthesis by up to 15% and underestimate transpiration by up to 12% with an associated increase in sensible heat fluxes since photosynthesis, transpiration and energy partitioning are tightly coupled processes. However, these test results need to be validated using the 3D architecture obtained from a real plant imaged in 3D using 3DArch, rather than an artificial model plant. Our proposed model framework would allow us to identify inefficiencies in canopy light distribution and prescribe optimal canopy architectures (leaf optical properties, leaf area, leaf angles, row spacing, planting density, etc.) to traditional and genetic engineering based crop breeders, for maximizing target functions such as light use efficiency, water use efficiency, drought tolerance, crop yield, crop resilience etc., with important applications in biofuel crops such as sorghum, and switchgrass. Our model can also be applied for designing optimal canopy architecture that increase surface albedo as a mitigation strategy for global warming and projections of crop yield for future food and water security under climate change.

59 BASIC BIOLOGICAL SCIENCES↗

A review of transformative strategies for climate mitigation by grasslands

Grasslands can significantly contribute to climate mitigation. However, recent trends indicate that human activities have switched their net cooling effect to a warming effect due to management intensification and land conversion. This indicates an urgent need for strategies directed to mitigate climate warming while enhancing productivity and efficiency in the use of land and natural (nutrients, water) resources. Here, in this work, we examine the potential of four innovative strategies to slow climate change including: 1) Adaptive multi-paddock grazing that consists of mimicking how ancestral herds roamed the Earth; 2) Agrivoltaics that consists of simultaneously producing food and energy from solar panels on the same land area; 3) Agroforestry with a reverse phenology tree species, Faidherbia (Acacia) albida, that has the unique trait of being photosynthetically active when intercropped herbaceous plants are dormant; and, 4) Enhanced Weathering, a negative emission technology that removes atmospheric CO 2 from the atmosphere. Further, we speculate about potential unknown consequences of these different management strategies and identify gaps in knowledge. We find that all these strategies could promote at least some of the following benefits of grasslands: CO 2 sequestration, non-CO 2 GHG mitigation, productivity, resilience to climate change, and an efficient use of natural resources. However, there are obstacles to be overcome. Mechanistic assessment of the ecological, environmental, and socio-economic consequences of adopting these strategies at large scale are urgently needed to fully assess the potential of grasslands to provide food, energy and environmental security.

54 ENVIRONMENTAL SCIENCES↗

Landfalling Droughts: Global Tracking of Moisture Deficits From the Oceans Onto Land

Abstract Droughts threaten food, energy, and water security, causing death and displacement of millions of people and billions of dollars in damages. However, there are still important gaps in the understanding of drought mechanisms and behaviors, inhibiting the accuracy of early‐warning systems designed to protect communities worldwide. We use an object‐tracking algorithm to track clusters of precipitation‐minus‐evaporation moisture deficits across land and ocean areas of the globe from 1981–2018. This analysis reveals a new type of “landfalling drought” that originates over the ocean and “migrates” onto land. We find that 16% of droughts that affected the continents worldwide from 1981–2018 were landfalling droughts. These droughts were significantly larger (220–425%) and more intense (4–30%)—and grew (253–285%) and intensified (9–28%) faster—than droughts that developed solely over the land or ocean. To identify potential underlying mechanisms, we analyze moisture transport associated with landfalling droughts over western North America. We find that landfalling droughts in this region are associated with anomalously anticyclonic atmospheric pressure patterns that reduce moisture fluxes over the Pacific Ocean toward the continent. By advancing understanding of the spatiotemporal evolution of droughts, our findings offer the potential to improve seasonal‐scale prediction and long‐term projection of global drought risks.

Herrera‐Estrada, Julio E.↗

Design of Stand-Alone Cryomodule based on Superconducting Quarter-Wave Resonator

Present superconducting accelerators require a central refrigeration system and piping to distribute liquid helium and nitrogen. Stand-alone cryocooler-based systems are of significant interest both to scientific and industrial customers, as they allow applications at facilities where no cryogenic equipment and expertise exists, where it is undesirable to develop additional cryogenic systems, or where a mobile system that can be transported between beamlines or facilities is required. The technical challenge of this approach is to minimize the heat losses to the liquid helium so that commercially available, self-contained cryocoolers are able to handle the thermal load. This paper presents an improved design for a stand-alone quarter-wave resonator (QWR) cryomodule a based on commercially available cryocoolers. This cryomodule has a particular application in the ATLAS intensity upgrade project where it will house a 72.75 MHz QWR for beam energy adjustments to experimental areas, where no helium cryogenic systems exist. The described cryomodule may also be the critical part of a turnkey system with application to material processing, semiconductor manufacturing, food irradiation and homeland security. The development of low heat loss peripheral components reduces cost and widens applicability of the system. We will discuss the cryomodule and RF coupler designs, including numerical thermal and structural analyses.

43 PARTICLE ACCELERATORS↗

Interdisciplinary Workshop on the Impacts of Land Use and Land Management on Earth System Evolution, Biogeochemical Cycles, Extremes, and Inter-Sectoral Dynamics (Final Report)

The Aspen Global Change Institute (AGCI) convened a week-long interdisciplinary science workshop from September 15-20, 2019 entitled, “Impacts of Land Use and Land Management on Earth System Evolution, Biogeochemical Cycles, Extremes, and Inter-Sectoral Dynamics.” The purpose of the workshop was to advance research on the impacts of land use and land management on climate, carbon emissions, and extremes, as well as the impact of land-use change on important societal issues such as food, water, and energy security. The workshop was co-chaired by Kate Calvin (PNNL), George Hurtt (University of Maryland), and Dave Lawrence (National Center for Atmospheric Research). Co-funders of the workshop included NASA, NOAA, and travel support for European colleagues through CRESCENDO. This workshop brought together 31 professionals with expertise across biogeochemistry, climate impacts, mitigation, land use datasets, and societal impacts to coordinate on upcoming research and publications on the role of biogeochemical and biophysical properties of the Earth surface and their implications for climate, as conducted through the Land Use model Intercomparison Project (LUMIP), which is part of Phase 6 of the Coupled Model Intercomparison Project (CMIP6). A primary outcome of the meeting was the definition and planning of a series of LUMIP papers that will rely on the LUMIP and CMIP6 simulations. By the end of the meeting more than twenty papers and paper lead authors had been identified and scoped out. Around ten of these papers were submitted by the December 31, 2019 IPCC deadline.

54 ENVIRONMENTAL SCIENCES↗

Small scale electron linear accelerators for industrial applications

Linear accelerators (linacs), producing electron or X-ray radiation in the MeV range, are critical tools for industrial irradiation, medical device sterilization, food pasteurization, non-destructive testing, security, medical, and many other applications. Many of these applications require compact and flexible radiation sources. In this paper, we present new technologies for small-scale electron linear accelerators and examples of their practical implementations. Furthermore, these developments include low energy accelerators with self-shielding options and form factors, ranging from cabinet-size to hand-portable generators; medium-energy accelerators for novel radiotherapy and security applications; and high-energy 10 MeV linacs capable of reaching 20–35 kW beam power for emerging industrial and phytosanitary applications

43 PARTICLE ACCELERATORS↗

Plant Single-Cell Solutions for Energy and the Environment (Second Workshop Report)

Plants are important sources of energy and materials, and they collectively represent a critical component of Earth’s ecosystem. With increasing environmental stresses due to climate change and intensive agricultural practices, the need for resilient plants is greater than ever before. To secure plant resources for bioenergy, biomaterials, food, and ecosystem adaptation, a deeper understanding of the fundamental biology of plants at a cellular level is urgently needed. Plants contain a multitude of specialized cell types that compose tissues and organs. Pathogens often target specific cell types within plants, and the response of one cell to a particular stimulus is likely to be distinct from its neighbor because of underlying molecular and contextual differences. Understanding how these responses are distributed among cells, the main goal of single-cell approaches, will substantially enhance our ability to use targeted engineering for improving plant productivity and resilience. Furthermore, single-cell approaches are necessary to understand the interactions between plants and other ecosystem members such as fungi, bacteria, and archaea. Unlocking these gene-response mechanisms at a cellular level can improve our ability to adapt plants to environmental stresses, increasing their utility as feedstocks for biomaterials and bioenergy. Recent advances in high-throughput sequencing, mass spectrometry, microfluidics and miniaturization, artificial intelligence and machine learning, and bioinformatics have greatly improved our ability to detect and understand processes at a cellular level. In mammalian systems, single-cell transcriptomics has already led to many advances, such as newly identified cell types and cell-targeted treatment of diseases, and mass spectrometry-based single-cell proteomics has recently been demonstrated as a promising emerging technology. However, plant single-cell omics has lagged behind mammalian approaches due to the high cost of the technologies relative to available resources and to the innate biological features of plants, including the complexity of the cell wall and polyploidy. To better understand how single-cell methods could enable plant science, Lawrence Berkeley National Laboratory (Berkeley Lab) hosted a workshop on April 29, 2021, that brought together a diverse group of leaders in plant and/or single-cell biology. Attendees represented federal research programs and domestic and international academic institutions. During the workshop, three presenters described the current state of research in both experimental and computational approaches. While the focus of the workshop was on factors preventing plant biology researchers from fully adopting single-cell methodologies, workshop participants agreed that most barriers could be overcome with focused, strategic investment and coordinated efforts among institutions leading to significant scientific discoveries that would be difficult to obtain using more conventional technologies.

59 BASIC BIOLOGICAL SCIENCES↗

Controlled environment agriculture: An opportunity to strengthen interagency research collaboration in the US government

Challenges facing food production and agricultural systems are increasingly interconnected with economic, security, health, and equity issues, among others. Threats such as extreme weather, economic volatility, and shrinking water resources and arable land, influence our ability to maintain a safe and resilient food supply. One promising solution to these threats is controlled environment agriculture (CEA). In many cases, CEA can drastically reduce the amount of water and land used in crop production while increasing productivity. Operations may be established in nearly any environment and harvests can take place year-round, supporting food system resiliency and sustainability. CEA sits at the nexus of a number of disciplines and industries, making it well suited for transdisciplinary and multi-institutional research coordination. Herein, authors from multiple US government agencies present CEA as a case study in improving cross-agency research collaboration. The federal government houses a range of scientific expertise and research capabilities, positioning scientists to lead national and global efforts in transdisciplinary, interagency approaches to complex challenges. Navigating cross-agency collaboration can be a challenge, especially coordinating across different scientific disciplines, geographic locations, and funding mechanisms. To enhance multiagency efforts, collaborators could prioritize (i) organizing personnel and resources, (ii) enhancing existing multiagency collaborations, and (iii) focusing on further opportunities for coordination. Adopting these approaches could enable federal researchers to reinforce and advance academic and industry efforts to address current CEA challenges while solidifying the United States as a leader in this arena.

29 ENERGY PLANNING, POLICY, AND ECONOMY↗

Plant Disease Detection Technology Assessment

Visual inspections by US Customs and Border Protection agriculture specialists have identified approximately 20,000 regulated, quarantined pests each year in agricultural products entering the United States. Most of these pests identified in the Agriculture Quarantine and Inspection (AQI) program are insects. Many pathogens are difficult to detect in agricultural products, particularly in early stages of infection. New technologies can help to detect plant pathogens and the diseases that they cause. This technology assessment was performed for the US Department of Homeland Security Science and Technology Directorate (DHS S&T) through the Food, Agriculture, and Veterinary Defense (FAV-D) program to identify emerging technologies that could address this hard problem. These emerging technologies differ in their diagnostic sensitivities and specificities, as well as in their measurement time and training requirements. New instruments that detect volatile organic compounds characteristic of plant disease or pathogens could provide a less invasive inspection method. Dogs, which can successfully detect many concealed agricultural products, have also been trained to detect some plant pests and pathogens. Simple immunological tests offer sensitive and specific detection of many pathogens at the point of use. Advanced imaging methods that use AI to sort fruits and vegetables and recognize anomalies at high speeds could be used in cooperation with exporters to improve food quality and reduce pests. Advances in nucleic acid–based detection methods that have become gold standards for confirmatory diagnostics are now making those methods available for faster, point-of-use detection. These new methods should be developed in the context of AQI operational requirements, which apply risk-based sampling protocols to protect agriculture and facilitate commerce and passenger transit.

59 BASIC BIOLOGICAL SCIENCES↗

Implication of stratospheric aerosol geoengineering on compound precipitation and temperature extremes in Africa

Three Coupled Model Intercomparison Project 5 (CMIP5) models that simulated the G4 experiment of the Geoengineering Model Intercomparison Project (GeoMIP) were used to investigate the impact of stratospheric aerosol injection (SAI) on combined temperature and precipitation extremes in Africa that can have greater negative impacts on human and the environment than individual rainfall or temperature extremes. The examined compound extremes included the dry (R warm|dry and R cold|dry ) and wet (R warm|wet and R cold|wet ) modes assessed during the injection (SAI, 2050-2069) and post-injection (postSAI, 2070-2089) periods compared with the historical period (1986-2005). We found a significant projected change in the occurrence of both wet and dry modes during SAI and postSAI related to the historical period. The magnitude and sign of this change depend on the season and the geographical location. During the SAI and postSAI, the wet (R warm|wet and R cold|wet ) modes are projected to be significantly lower while the dry modes are noted to increase in a large part of African continent depending on the season and the geographical location and may consequently leads to an increase of the droughts prone areas. Furthermore, the termination effect is noted to re duce the occurrence of dry modes, which may reduce the potential negative effects of the injection after halting. As the effect may vary from one region to another and according to the season, it suggested assessing the key sector impacts of SAI. Thus, this change in dry modes due to SAI could affect all activities which depend on water resources such as water supply, agriculture and food production, energy demand, and production with adverse effects on health, security, and sustainable development, but this needs to be assessed and quantified at regional scales.

54 ENVIRONMENTAL SCIENCES↗

CRITICAL FUNCTION ASSURANCE: Understanding Critical Function and Critical Function Delivery is Foundational for Meaningful ICS Security Improvement and Policy Efforts

Modern life is enabled by a complex and interdependent web of critical functions, including energy, communications, transportation, food, and water. Automation has significantly reduced or replaced human interactions in the delivery of these functions, resulting in a web of goods and services that are made available 24/7 only through unique and intentional deployments of microprocessors, software, and firmware technologies. The prospect of cyber-enabled sabotage of these processes disrupts traditional risk determination models. Critical Function Assurance (CFA) is a foundational approach to identifying, prioritizing, and mitigating the risk that is inherent in the delivery of critical functions that depend on digital technology. It provides rapid focus to what matters most and illuminates elements and areas of risk that otherwise are often overlooked. This focus enables effective application of available security resources and optimizes security strategy and policy efforts. This paper introduces CFA to decision makers and risk executives (including CEOs, COOs, CFOs, and CISOs) whose organizations support and deliver the critical functions that underpin national defense, societal health and safety, and a vibrant economy.

29 ENERGY PLANNING, POLICY, AND ECONOMY↗

Ameliorating Global Challenges: Globalization, Geopolitics, Basic & Applied Research, and Research Security

We are confronted with a myriad of global challenges, from extreme weather events, occurring at higher frequencies than at any point in history, to pollution, food insecurity, clean water shortages, and fundamentally limited natural resources and materials. The largest number of people inhabit our planet today and enjoy the highest standard of living - though not equally distributed across the world - compared to any prior moment in history. To sustain this quality of life, we are largely reliant on fossil fuel sources, which are responsible for more greenhouse gas emissions by weight each day than the collective weight of all humans that inhabit the planet. Ameliorating these global challenges will require elements of solutions that include advances in basic and applied research, innovative global engineering, materials discovery, new technologies, manufacturing at scale, resilient and adaptable infrastructure, carbon-free energy sources and storage technologies, and new supply chain networks and markets. Success will require constructive collaborative efforts between researchers in countries located in every continent of this planet. For any of these goals to be realized in a timely fashion, geopolitical leaders must become better educated about this existential challenge and incentivized to act.

applied research↗