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At least 145 records · Page 8
All sourcesNASA NTRSDOE OSTI
DOE OSTI2022

Alphavirus Particles Can Assemble with an Alternate Triangulation Number

Alphaviruses are spherical, enveloped RNA viruses primarily transmitted by mosquitoes, and cause significant arthritogenic and neurotropic disease in humans and livestock. Previous reports have shown that—in contrast to prototypical icosahedral viruses—alphaviruses incorporate frequent defects, and these may serve important functions in the viral life cycle. We confirm the genus-wide pleomorphism in live viral particles and extend our understanding of alphavirus assembly through the discovery of an alternate architecture of Eastern equine encephalitis virus (EEEV) particles. The alternate T = 3 icosahedral architecture differs in triangulation number from the classic T = 4 icosahedral organization that typifies alphaviruses, but the alternate architecture maintains the quasi-equivalence relationship of asymmetric units. The fusion spike glycoproteins are more loosely apposed in the T = 3 form with corresponding changes in the underlying capsid protein lattice. This alternate architecture could potentially be exploited in engineering alphavirus-based particles for delivery of alphaviral or other RNA.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2024

A Self-Assembling Pfs230D1-Ferritin Nanoparticle Vaccine Has Potent and Durable Malaria Transmission-Reducing Activity

Malaria is caused by eukaryotic protozoan parasites of the genus Plasmodium. There are 249 million new cases and 608,000 deaths annually, and new interventions are desperately needed. Malaria vaccines can be divided into three categories: liver stage, blood stage, or transmission-blocking vaccines. Transmission-blocking vaccines prevent the transmission of disease by the mosquito vector from one human to another. Pfs230 is one of the leading transmission-blocking vaccine antigens for malaria. Here, we describe the development of a 24-copy self-assembling nanoparticle vaccine comprising domain 1 of Pfs230 genetically fused to H. pylori ferritin. The single-component Pfs230D1-ferritin construct forms a stable and homogenous 24-copy nanoparticle with good production yields. The nanoparticle is highly immunogenic, as two low-dose vaccinations of New Zealand White rabbits elicited a potent and durable antibody response with high transmission-reducing activity when formulated in two distinct adjuvants suitable for translation to human use. This single-component 24-copy Pfs230D1-ferritin nanoparticle vaccine has the potential to improve production pipelines and the cost of manufacturing a potent and durable transmission-blocking vaccine for malaria control.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2021

Structural basis of malaria transmission blockade by a monoclonal antibody to gamete fusogen HAP2

HAP2 is a transmembrane gamete fusogen found in multiple eukaryotic kingdoms and is structurally homologous to viral class II fusogens. Studies in Plasmodium have suggested that HAP2 is an attractive target for vaccines that block transmission of malaria. HAP2 has three extracellular domains, arranged in the order D2, D1, and D3. Here, we report monoclonal antibodies against the D3 fragment of Plasmodium berghei HAP2 and crystal structures of D3 in complex with Fab fragments of two of these antibodies, one of which blocks fertilization of Plasmodium berghei in vitro and transmission of malaria in mosquitoes. We also show how this Fab binds the complete HAP2 ectodomain with electron microscopy. The two antibodies cross-react with HAP2 among multiple plasmodial species. Our characterization of the Plasmodium D3 structure, HAP2 ectodomain architecture, and mechanism of inhibition provide insights for the development of a vaccine to block malaria transmission.

59 BASIC BIOLOGICAL SCIENCES↗
NASA NTRS1972

Public health applications of remote sensing

Remote infrared and multispectral photography were used to identify coastal salt water-fresh water interfaces conducive to encephalitis vector mosquito breeding in Florida, and to determine the environmental conditions that caused an explosive outbreak of anthrax in Louisiana. Multiband photographic inventories were obtained by simultaneously processing three photographic negatives of the same view which record different wavelength portions of the same light. The process enhances differentiation of vegetative communities and sharply delineates edge effects by assigning false colors to differentiate subtle density differences.

Fuller, C. E.↗
NASA NTRS1972

Application of ecological, geological and oceanographic ERTS-1 imagery to Delaware's coastal resources planning

The author has identified the following significant events. Coastal vegetation species appearing in the ERTS-1 image taken of the Southern Coast of Delaware, during orbit 333 on August 16, 1972, have been correlated with ground truth vegetation maps, and imagery obtained from high altitude RB-57 and U-2 overflights. The vegetation maps of the entire Delaware Coast were prepared using data collected on foot, in small boats, and from low altitude aircraft. Multispectral analysis of high altitude RB-57 and U-2 photographs indicated that five vegetation species could be clearly discriminated from 60,000 feet altitude, including: (1) salt marsh cord grass (Spartina alterniflora); (2) salt marsh hay and spike grass (Spartina patens and Distichlis spicata); (3) reed grass (Phragmites communis); (4) high tide bush and sea myrtle (Iva species and Baccharus halimifolia); and (5) a group of fresh water species found in impounded areas built to attract water fowl. Major Spartina alterniflora and Spartina patens communities within the tidal marshes can be identified in the ERTS-1 imagery. Phragmites, and other species however, occur in smaller, more dispersed groupings and are difficult to discriminate within the resolution capability of the ERTS-1 scanner. Similarly, major impounded areas, built to attract water fowl can be detected; however, mosquito drainage ditches, covering many of Delaware's marshes, are too narrow and not long enough to be resolved by ERTS-1 sensors. High-marsh and dune communities dominated by high tide bush (Iva frutescens) and sea myrtle (Baccharus halimifolia) can be distinguished from adjacent maritime forest and beach grass communities.

Klemas, V.↗
NASA NTRS1973

Mapping northern Atlantic coastal marshlands, Maryland-Virginia, using ERTS imagery

The author has identified the following significant results. ERTS-1 data provides repetitive synoptic coverage for DC 00000 of wetland ecology, detection of change, and mapping or inventory of wetland boundaries and plant communities. ERTS-1 positive transparencies of Atlantic Coastal wetlands were enlarged to different scales and mapped using a variety of methods. Results of analysis indicate: (1) mapping of wetland boundaries and vegetative communities from imagery at a scale of 1:1,000,000 is impractical because small details are difficult to illustrate; (2) mapping to a scale of 1:250,000 is practical for defining land-water interface, upper wetland boundary, gross vegetative communities, and spoil disposal/dredge and fill operations; (3) 1:125,000 enlargements provide additional information on transition zones, smaller plant communities, and drainage or mosquito ditching. Overlays may be made directly from prints.

Anderson, R. R.↗
NASA NTRS1973

Application of ecological, geological and oceanographic ERTS-1 imagery to Delaware's coastal resources planning

The author has identified the following significant results. Coastal vegetation species appearing in the ERTS-1 images taken of Delaware Bay have been correlated with ground truth vegetation maps and imagery obtained from high altitude overflights. Multispectral analysis of the high altitude photographs indicated that four major vegetation communities could be clearly discriminated from 60,000 feet altitude including: (1) salt marsh cord grass; (2) salt marsh hay and spike grass; (3) reed grass; and (4) high tide bush and sea myrtle. In addition, human impact can be detected in the form of fresh water impoundments built to attract water fowl, dredge-fill operations and other alterations of the coastal environment. Overlay maps matching the USGS topographic map size of 1:24,000 have been prepared showing the four wetland vegetation communities, fresh water impoundments, and alteration of wetlands by mosquito control ditching and dredge-fill operations. Using these maps, ERTS-1 images were examined by human interpreters and automated multispectral analyzers. Major plant communities of (1) Spartina alterniflora, (2) Spartina patens and Distichlis spicata, and (3) Iva frutescens and Baccharis halimifolia can be distinguished from each other and from surrounding uplands in ERTS-1 scanner bands 6 and 7.

Klemas, V.↗
NASA NTRS1973

Kennedy Space Center ocean beach erosion

Dune barrier erosion and possible breakthrough due to storm and hurricane wave activity is studied near Mosquito Lagoon, in Kennedy Space Center property. The results of a geological as well as hydrodynamic appraisal of the problem area indicate that no inlet has existed across the dune barrier since 500 A.D., and that there is little likelihood of a possible breakthrough inlet remaining open permanently, primarily because the relatively shallow lagoon does not contain enough volume of water to maintain an inlet between the ocean and the lagoon. It is therefore recommended that only minimal measures, such as closing up the man-made passes across the dunes, be carried out to ensure continuation of the action of natural beach maintaining processes.

Mehta, A. J.↗
NASA NTRS1975

Application of remote sensor data to geologic analysis of the Bonanza test site Colorado

Selected samples of anomalous surface features commonly associated with the various types of uranium deposits are presented and recommendations for sensor applications are given. The features studied include: epigenetic uranium ore roll type; precambrian basal conglomerate type; vein-type uranium deposits; pipe-structure or diatreme deposits; evaporitic uranium deposits. The hydrogeology of the Mosquito Range and the San Luis Valley is also examined.

Lee, K.↗
NASA NTRS1976

Public health applications of remote sensing of vector borne and parasitic diseases

Results of an investigation of the potential application of remote sensing to various fields of public health are presented. Specific topics discussed include: detection of snail habitats in connection with the epidemiology of schistosomiasis; the detection of certain Anopheles breeding sites, and location of transient human populations, both in connection with malaria eradication programs; and detection of overwintering population sites for the primary screwworm (Cochliomyia americana). Emphasis was placed on the determination of ground truth data on the biological, chemical, and physical characteristics of ground waters which would or would not support the growth of significant populations of mosquitoes.

Source record↗
NASA NTRS1977

Use of remote sensing for land use policy formulation

The use of remotely sensed data for eliminating abuses and mismanagement of land and water resources in Michigan is discussed. Applications discussed include inventory of mosquito breeding sites; analysis of biomass in old field ecosystems used for wastewater recycling; areas for agricultural use; and preservation of the Grand Mere Dune environment. Services to users are described and contact activities reported.

Boylan, M.↗
NASA NTRS1977

Application of aerial photography to water-related programs in Michigan

Aerial photography and information system technology were used to generate information required for the effective operation of three water-related programs in Michigan. Potential mosquito breeding sites were identified from specially acquired low altitude 70 mm color photography for the city of Lansing; the inventory identified 35% more surface water areas than indicated on existing field maps. A comprehensive inventory of surface water sources and potential access sites was prepared to assist fire departments in Antrim County with fire truck water-recharge operations. Remotely-sensed land cover/use data for Windsor Township, Eaton County, were integrated with other resource data into a computer-based information system for regional water quality studies. Eleven thematic maps focusing on landscape features affecting non-point water pollution and waste disposal were generated from analyses of a four-hectare grid-based data file containing land cover/use, soils, topographic and geologic (well-log) data.

Enslin, W. R.↗
NASA NTRS1977

Application of aerial photography to water-related programs in Michigan

The paper describes the use of aerial photography and information system technology in the provision of information required for the effective operation of three water-related programs in Michigan. Potential mosquito breeding sites were identified from specially acquired low altitude 70 mm color photography for the City of Lansing Vector Control Area. A comprehensive inventory of surface water sources and potential access sites was prepared to assist fire departments in Antrim County with fire truck water-recharge operations. Remotely-sensed land cover/use data for Windsor Township, Eaton County were integrated with other resource data into a computer-based information system for regional water quality studies. Eleven thematic maps specifically focussed on landscape features affecting non-point water pollution and waste disposal were generated from analyses of a four-hectare grid-based data file containing land cover/use, soils, topographic and geologic (well-log) data.

Enslin, W. R.↗
NASA NTRS1980

The applicability of remote sensing to Earth biological problems. Part 2: The potential of remote sensing in pest management

Five troublesome insect pest groups were chosen for study. These represent a broad spectrum of life cycles, ecological indicators, pest management strategies, and remote sensing requirements. Background data, and field study results for each of these subjects is discussed for each insect group. Specific groups studied include tsetse flies, locusts, western rangeland grasshoppers, range caterpillars, and mosquitoes. It is concluded that remote sensing methods are aplicable to the pest management of the insect groups studied.

Polhemus, J. T.↗
NASA NTRS1982

Doppler-radar observation of the evolution of downdrafts in convective clouds

A detailed analysis of the 20 July 1977 thunderstorm complex which formed and evolve over the South Park region in Central Colorado is presented. The storm was extensively analyzed using multiple Doppler radar and surface mesonet data, developed within an environment having very weak wind shear. The storm owed its intensification to the strength of the downdraft, which was nearly coincident with the region where the cloud had grown. The noteworthy features of this storm were its motion to the right of the cloud-level winds, its multicellular nature and discrete propagation, its north-south orientation, and its relatively large storm size and high reflectivity factor (55 dBZ). This scenario accounts for the observed mesoscale and cloud-scale event. A line of convergence was generated at the interface between the easterly upslope winds and westerly winds. During stage II, the convergence line subsequently propagated down the slopes of the Mosquito Range, and was the main forcing mechanism for the development of updraft on the west flank of the storm. The formation of downdraft on the eastern side of updraft blacked surface inflow, and created a detectable gust front. As the original downdraft intensified, the accumulation of evaporatively-chilled air caused the intensification of the mesohigh, which likely destroyed the earlier convergence line and created a stronger convergence line to the east, which forced up-lifting of the moist, westerly inflow and caused the formation of updraft to the east. An organized downdraft circulation, apparently maintained by precipitation drag and evaporational cooling, was responsible in sustaining a well-defined gust front. The storm attained its highest intensity as a consequence of merging with a neighboring cloud. The interaction of downdrafts or gust fronts from two intense cells appeared to be the primary mechanism of this merging process as suggested by Simpson et al. (1980). The merging process coincided with more rain than occurred in unmerged echoes.

Motallebi, N.↗
NASA NTRS1989

Rocket-triggered lightning strikes and forest fire ignition

Background information on the rocket-triggered lightning project at Kennedy Space Center (KSC), a summary of the forecasting problem there, the facilities and equipment available for undertaking field experiments at KSC, previous research activity performed, a description of the atmospheric science field laboratory near Mosquito Lagoon on the KSC complex, methods of data acquisition, and present results are discussed. New sources of data for the 1989 field experiment include measuring the electric field in the lower few thousand feet of the atmosphere by suspending field measuring devices below a tethered balloon. Problems encountered during the 1989 field experiment are discussed. Future prospects for both triggered lightning and lightning-kindled forest fire research at KSC are listed.

Fenner, James H.↗
NASA NTRS1990

Rocket-triggered lightning strikes and forest fire ignition

The following are presented: (1) background information on the rocket-triggered lightning project an Kennedy Space Center (KSC); (2) a summary of the forecasting problem; (3) the facilities and equipment available for undertaking field experiments at KSC; (4) previous research activity performed; (5) a description of the atmospheric science field laboratory near Mosquito Lagoon on the KSC complex; (6) methods of data acquisition; and (7) present results. New sources of data for the 1990 field experiment include measuring the electric field in the lower few thousand feet of the atmosphere by suspending field measuring devices below a tethered balloon, and measuring the electric field intensity in clouds and in the atmosphere with aircraft. The latter program began in July of 1990. Also, future prospects for both triggered lightning and forest fire research at KSC are listed.

Fenner, James↗
NASA NTRS1992

Identification of central Kenyan Rift Valley Fever virus vector habitats with Landsat TM and evaluation of their flooding status with airborne imaging radar

Rift Valley Fever (RVF) is a mosquito-borne virus that affects livestock and humans in Africa. Landsat TM data are shown to be effective in identifying dambos, intermittently flooded areas that are potential mosquite breeding sites, in an area north of Nairobi, Kenya. Positive results were obtained from a limited test of flood detection in dambos with airborne high resolution L, C, and X band multipolarization SAR imagery. L and C bands were effective in detecting flooded dambos, but LHH was by far the best channel for discrimination between flooded and nonflooded sites in both sedge and short-grass environments. This study demonstrates the feasibility of a combined passive and active remote sensing program for monitoring the location and condition of RVF vector habitats, thus making future control of the disease more promising.

Pope, K. O.↗