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At least 145 records · Page 8

Nutrient Stress Detection in Corn Using Neural Networks and AVIRIS Hyperspectral Imagery

AVIRIS image cube data has been processed for the detection of nutrient stress in corn by both known, ratio-type algorithms and by trained neural networks. The USDA Shelton, NE, ARS Variable Rate Nitrogen Application (VRAT) experimental farm was the site used in the study. Upon application of ANOVA and Dunnett multiple comparsion tests on the outcome of both the neural network processing and the ratio-type algorithm results, it was found that the neural network methodology provides a better overall capability to separate nutrient stressed crops from in-field controls.

Estep, Lee↗

The effect of drying and size reduction pretreatments on recovery of inorganic crop nutrients from inedible wheat residues

Inorganic nutrients can be easily recovered from ALS crop residue solid wastes by aqueous leaching. However, oven drying and milling pretreatment of these residues has been frequently required to accommodate crop scientists and facility storage limitations. As part of a research study that will compare three different bioreactor technologies for processing these wastes, we realized that different drying and size-reduction pretreatments had been utilized for each technology. This paper compares the effects of residue pretreatment on recovery of nutrients by leaching. Pretreatments included three drying methods [fresh, oven-dried (70 degrees C overnight), and freeze-dried] and two size reduction methods [chopped (2 cm length) and milled (2 mm diameter)]. Determination of mass balances (dry weight and ash content of solids) before and after leaching indicated solubilization was least for fresh residues (23% dry weight loss and 50% for ash loss), and most for freeze-dried residues (41-47% dry weight loss and nearly 100% for ash loss). Mineral recovery of major elements (NO3, PO4, K, Ca, and Mg) in leachates was poorest for fresh residues. P and K recovery in leachates were best for oven-dried residues and Ca, Mg, and N recovery best for freeze-dried residues. The differences in recovery for N, P, and K in leachates were minimal between chopping and milling and slightly better for Ca and Mg from milled residues.

NASA Center KSC↗

Effect of hydraulic retention time on inorganic nutrient recovery and biodegradable organics removal in a biofilm reactor treating plant biomass leachate

A fixed-film (biofilm) reactor was designed and its performance was determined at various retention times. The goal was to find the optimal retention time for recycling plant nutrients in an advanced life support system, to minimize the size, mass, and volume (hold-up) of a production model. The prototype reactor was tested with aqueous leachate from wheat crop residue at 24, 12, 6, and 3 h hydraulic retention times (HRTs). Biochemical oxygen demand (BOD), nitrates and other plant nutrients, carbohydrates, total phenolics, and microbial counts were monitored to characterize reactor performance. BOD removal decreased significantly from 92% at the 24 h HRT to 73% at 3 h. Removal of phenolics was 62% at the 24 h retention time, but 37% at 3 h. Dissolved oxygen concentrations, nitric acid consumption, and calcium and magnesium removals were also affected by HRT. Carbohydrate removals, carbon dioxide (CO2) productions, denitrification, potassium concentrations, and microbial counts were not affected by different retention times. A 6 h HRT will be used in future studies to determine the suitability of the bioreactor effluent for hydroponic plant production.

NASA Center KSC↗

Approaches in the determination of plant nutrient uptake and distribution in space flight conditions

The effective growth and development of vascular plants rely on the adequate availability of water and nutrients. Inefficiency in either the initial absorption, transportation, or distribution of these elements are factors which impinge on plant structure and metabolic integrity. The potential effect of space flight and microgravity conditions on the efficiency of these processes is unclear. Limitations in the available quantity of space-grown plant material and the sensitivity of routine analytical techniques have made an evaluation of these processes impractical. However, the recent introduction of new plant cultivating methodologies supporting the application of radionuclide elements and subsequent autoradiography techniques provides a highly sensitive investigative approach amenable to space flight studies. Experiments involving the use of gel based 'nutrient packs' and the radionuclides calcium-45 and iron-59 were conducted on the Shuttle mission STS-94. Uptake rates of the radionuclides between ground and flight plant material appeared comparable.

manned↗

An evaluation of MES (2(N-Morpholino)ethanesulfonic acid) and Amberlite IRC-50 as pH buffers for nutrient solution studies

All buffering agents used to stabilize pH in hydroponic research have disadvantages. Inorganic buffers are absorbed and may become phytotoxic. Solid carbonate salts temporarily mitigate decreasing pH but provide almost no protection against increasing pH, and they alter nutrient absorption. Exchange resins are more effective, but we find that they remove magnesium and manganese from solution. We have tested 2(N-Morpholino)ethanesulfonic acid (MES) as a buffering agent at concentrations of 1 and 10 mol m-3 (1 and 10 mM) with beans, corn, lettuce, tomatoes, and wheat. MES appears to be biologically inert and does not interact significantly with other solution ions. Relative growth rates among controls and MES treatments were nearly identical for each species during the trial period. The pH was stabilized by 1 mol m-3 MES. This buffer warrants further consideration in nutrient research.

Alkanesulfonic Acids/chemistry/pharmacology↗

Through form to function: root hair development and nutrient uptake

Root hairs project from the surface of the root to aid nutrient and water uptake and to anchor the plant in the soil. Their formation involves the precise control of cell fate and localized cell growth. We are now beginning to unravel the complexities of the molecular interactions that underlie this developmental regulation. In addition, after years of speculation, nutrient transport by root hairs has been demonstrated clearly at the physiological and molecular level, with evidence for root hairs being intense sites of H(+)-ATPase activity and involved in the uptake of Ca(2+), K(+), NH(4)(+), NO(3)(-), Mn(2+), Zn(2+), Cl(-) and H(2)PO(4)(-).

Review, Tutorial↗

Improved Hypoxia Modeling for Nutrient Control Decisions in the Gulf of Mexico

The Gulf of Mexico Modeling Framework is a suite of coupled models linking the deposition and transport of sediment and nutrients to subsequent bio-geo chemical processes and the resulting effect on concentrations of dissolved oxygen in the coastal waters of Louisiana and Texas. Here, we examine the potential benefits of using multiple NASA remote sensing data products within this Modeling Framework for increasing the accuracy of the models and their utility for nutrient control decisions in the Gulf of Mexico. Our approach is divided into three components: evaluation and improvement of (a) the precipitation input data (b) atmospheric constituent concentrations in EPA's air quality/deposition model and (c) the calculation of algal biomass, organic carbon and suspended solids within the water quality/eutrophication models of the framework.

Habib, Shahid↗

Examining Dehydration and Hypoxic Stress in Wheat Plants Using a Porous Tube Plant Nutrient Delivery System Developed for Microgravity

The Porous Tube Plant Nutrient Delivery System (PTPNDS) was designed for NASA to grow plants in microgravity of space. The system utilizes a controlled fluid loop to supply nutrients and water to plant roots growing on a ceramic surface moistened by capiflary action. A PTPNDS test bed was developed and utilizing remote sensing systems, spectral analyses procedures, gas-exchange, and fluorescence measurements, we examined differences in plant water status for wheat plants (Triticum aestivum, cv. Perigee) grown in a modified growth chamber during the summers of 2003 and 2004. Some differences in plant performance were detectable in the gas-exchange and fluorescence measurements. For instance, in both years the plants grown with the most available water had the lowest rates of photosynthesis and exhibited higher proportions of non-photochemical quenching particularly under low light levels. In addition, small differences in mean leaf water content between treatments were detected using spectral reflectance analyses.

Dreschel, T. W.↗

A History of Vegetation, Sediment and Nutrient Dynamics at Tivoli North Bay, Hudson Estuary, New York

We conduct a stratigraphic paleoecological investigation at a Hudson River National Estuarine Research Reserve (HRNERR) site, Tivoli Bays, spanning the past 1100 years. Marsh sediment cores were analyzed for ecosystem changes using multiple proxies, including pollen, spores, macrofossils, charcoal, sediment bulk chemistry, and stable carbon and nitrogen isotopes. The results reveal climatic shifts such as the warm and dry Medieval Warm Period (MWP) followed by the cooler Little Ice Age (LIA), along with significant anthropogenic influence on the watershed ecosystem. A five-fold expansion of invasive species, including Typha angustifolia and Phragmites australis, is documented along with marked changes in sediment composition and nutrient input. During the last century, a ten-fold sedimentation rate increase due to land-use changes is observed. The large magnitude of shifts in vegetation, sedimentation, and nutrients during the last few centuries suggest that human activities have made the greatest impact to the marshes of the Hudson Estuary during the last millennium. Climate variability and ecosystem changes similar to those observed at other marshes in northeastern and mid-Atlantic estuaries, attest to the widespread regional signature recorded at Tivoli Bays.

Sritrairat, Sanpisa↗

Estimating Major Ion and Nutrient Concentrations in Mangrove Estuaries in Everglades National Park Using Leaf and Satellite Reflectance

Coastal mangrove ecosystems are under duress worldwide because of urban development, sea-level rise, and climate change, processes that are capable of changing the salinity and nutrient concentration of the water utilized by the mangroves. This study correlates long-term water chemistry in mangrove environments, located in Everglades National Park,with mangrove spectral reflectance measurements made at both the leaf and canopy scales. Spectral reflectance measurements were collected using a handheld spectrometer for leaf-level measurements and Landsat 5TMdata for regional coverage. Leaf-level reflectance data were collected from three mangrove species (i.e., red, black and white mangroves) across two regions; a tall mangrove (approximately 18m) and dwarf mangrove (1- 2 m) region. The reflectance data were then used to calculate a wide variety of biophysical reflectance indices (e.g., NDVI, EVI, SAVI) to determine signs of stress. Discrete, quarterly water samples from the surface water, groundwater, and pore water (20 and 85 cm depths) and daily autonomous surface water samples were collected at each site and analyzed for major anions (Cl(−) and SO(2(-)/4)), cations (Na(+), K(+), Mg(sup 2(+)), and Ca(sup 2(+)), total nitrogen (TN) and total phosphorus (TP).Mangrove sites that exhibited the highest salinity and ionic concentrations in the surface and subsurface water also had the lowest near-infrared reflectance at both the leaf and satellite levels. Seasonal reflectance responses were measured in the near-infrared (NIR) wavelengths at both the leaf and canopy scales and were strongly correlated with nutrient and ionic concentrations in the surface and subsurface water, even though there was no significant separability between the three mangrove species. Study sites that experienced the greatest variability in surface and subsurface water ionic concentrations also exhibited the greatest fluctuations in NIR spectral reflectance. Landsat 5TM images were able to detect tall and dwarf mangroves by the differences in spectral indices (e.g., NDVI, NDWI, and EVI) because of the variability in the background conditions amongst the environments. In addition, Landsat 5TM images spanning 16 years (1993- 2009) were successfully used to estimate the seasonal variability in ionic concentrations in the surface water across the Florida Coastal mangrove ecotone. This study has shown that water chemistry can be estimated indirectly by measuring the change in spectral response at the leaf- or satellite-scale. Furthermore, the results of this research may be extrapolated to similar coastal mangrove systems throughout the Caribbean and world-wide wherever red, black, and white mangroves occur.

Mangroves↗

Global Patterns of Crop Yield Stability Under Additional Nutrient and Water Inputs

Agricultural production must increase to feed a growing and wealthier population, as well as to satisfy increasing demands for biomaterials and biomass-based energy. At the same time, deforestation and land-use change need to be minimized in order to preserve biodiversity and maintain carbon stores in vegetation and soils. Consequently, agricultural land use needs to be intensified in order to increase food production per unit area of land. Here we use simulations of AgMIP's Global Gridded Crop Model Intercomparison (GGCMI) phase 1 to assess implications of input-driven intensification (water, nutrients) on crop yield and yield stability, which is an important aspect in food security. We find region- and crop-specific responses for the simulated period 1980+/-2009 with broadly increasing yield variability under additional nitrogen inputs and stabilizing yields under additional water inputs (irrigation), reflecting current patterns of water and nutrient limitation. The different models of the GGCMI ensemble show similar response patterns, but model differences warrant further research on management assumptions, such as variety selection and soil management, and inputs as well as on model implementation of different soil and plant processes, such as on heat stress, and parameters. Higher variability in crop productivity under higher fertilizer input will require adequate buffer mechanisms in trade and distribution/storage networks to avoid food price volatility.

Maize↗

Irrigation System for Microgravity (Candidate): The Passive Porous Tube Nutrient Delivery System (PPTNDS)

In an effort to supplement astronauts’ diets with fresh produce during future long-term space missions, the team at NASA Kennedy Space Center is working on methods to better deliver water and nutrients to plant root zones, with the desire to create a more reliable vegetable production system. Effectively doing this in microgravity has proven to be one of the biggest challenges experienced. Here, we detail the development and testing of the Passive Porous Tube Nutrient Delivery System (PPTNDS).

Jacob Torres↗

BioNutrients-2: Improvements to the BioNutrients-1 Nutrient Production System

The BioNutrients (BN) project aims to develop an on-demand biological production system capable of rapid and safe delivery of multiple nutrients in single-use production packs for long-duration missions. The general concept of this system is to develop edible engineered microorganisms that produce nutrients for human consumption following long-term storage, revival, and growth. BN-1 is a five-year flight project currently on the International Space Station (ISS). It is testing the long-term storage of various microorganisms for the biomanufacturing of space-relevant compounds and validating the performance of the first generation of production packs. The BN-2 project expands the BN-1 flight project scope by introducing novel products, broadening the range of microbial food sources, and improving upon production pack hardware. We are optimizing the biomanufacturing platform for in situ production of yogurt and kefir on the ISS. BN-2 also will test engineered microorganisms, including a probiotic strain, to produce carotenoids or follistatin. To improve the feasibility of this technology for mission use, we transitioned from a hard-shell pack to a second-generation fluorinated ethylene propylene (FEP) bag. The single-use FEP bioreactor facilitates growth of target organisms in a contaminant free environment, while reducing mass and volume compared to the BN-1 hardware. These bioreactors will be employed for production of additional space-relevant products in the BN-2 project. On-orbit testing of FEP production packs will enable optimization of the platform for eventual flight use by the crew.

Natalie Ball↗

Nutrient Stability in NASA Spaceflight Experiment Rodent Food Bars

The nutrient-upgraded Rodent Food Bars (NuRFB) have a long history as the food of choice for NASA Space Shuttle and ISS Rodent Research missions. The NuRFB have previously been assigned an expiration date of two years after production for use in rodent flight experiments aboard the International Space Station (ISS) and three years for ground experiment use when stored between 2°-10°C. The exiguous supply of cold stowage on the ISS necessitated further investigation to determine if NuRFB can be stored at ambient temperatures (AT) without excessive nutrient degradation. The Rodent Research Project Sustaining Engineering Team at the NASA Ames Research Center in Mountain View, CA is responsible for management of this critical flight product.

Rodent Research↗

KSC to EDEN ISS: Outredgeous Lettuce Growth on the Passive Porous Tube Nutrient Delivery System (PPTNDS)

EDEN ISS is a controlled environment greenhouse established near the Neumayer Station III in Antarcticawith the mission to enable fresh crop production for the crew who are working in this remote location. EDEN provides a space to conduct plant biology and related research in a unique and isolated controlled environment (CE), while providing a platform to test developing methods relevant to long duration manned space missions. In 2021, Kennedy Space Center (KSC) scientist Jess Bunchek fulfilled a one-year mission at EDEN. During that time a variety of fresh crops were grown to record horticultural and microbiological data as well as provide the crew with fresh food to eat. One method for crop growth included an experimental irrigation system called the Passive Porous Tube Nutrient Delivery System (PPTNDS) which was developed at KSC as a microgravity-capable plant irrigation system designed to support Space Plant Biology investigations and/or crop growth during space missions. The PPTNDS was employed to grow Outredgeous Lettuce (OL) during two growouts at EDEN, (harvesting at Day After Planting (DAP) 61 and 63) and two growouts at KSC where plant growth and micro sample data were captured. This paper presents the data collected from the PPTNDS and compares it to growth on a Nutrient Film Technique (NFT) hydroponic system operated in the same environment. The result shows a system that grows OL at a reduced rate compared to the NFT system but uses less water and crew interaction to operate.

Jacob Torres↗

The Future of Astronaut Nutrition: Daily Production of Kefir Nutrient Packs in a Lunar Analog Mission

Astronaut nutrition will need to be supplemented with fresh nutrients and probiotics for long duration space travel. Kefir is a fermented milk beverage that provides probiotic bacteria, protein, calcium, and vitamin K. It has been historically cultured for thousands of years and is a modern-day health food. During the BioNutrients-2 experiment, one generation of kefir was cultured by astronauts on the ISS in fluorinated ethylene propylene (FEP) bags. This follow-on experiment aimed to test the hypothesis that kefir can be produced at ambient temperatures and safely passaged to make a series of nutrient packs in an analog environment. The Hawaiian Space Exploration Analog and Simulation (HI-SEAS) is a semi-controlled habitat located on the Mauna Loa volcano on the island of Hawaii at 2,500 m elevation. The recent EMMIHS (EuroMoonMars, International MoonBase Alliance, HI-SEAS) missions comprised of interdisciplinary, international crews for mock lunar missions. During a six-day mission in March 2024, crew members tested continuous passaging of kefir cultures (C-FIR commercial strain). After approximately 24 hours of growth, a portion of culture was used to seed the next generation of culture bags for four passages. A color board and pH indicator allowed the crew to easily determine when the culture had reached optimal pH. Laboratory analysis at NASA Ames Research Center showed that the pH met Kefir standards (<5). The yeast and lactic acid bacteria were in normal range and no contamination was detected in the final passage. By successfully accomplishing this experiment the team has demonstrated the ability to safely produce daily cultures of kefir in a microgravity applicable growth system.

Astronaut nutrition↗

Spatiotemporal Dynamics of the Relative Abundance of Soil Nutrient‐Degrading Enzyme‐Encoding Genes Across Continental US Ecoregions

Understanding the spatiotemporal patterns in the relative abundance of soil extracellular enzyme‐encoding genes is critical for predicting microbial responses to environmental change and their potential role in nutrient cycling. Yet, integrating novel metagenomic observations with spatiotemporal environmental gradients to infer regional patterns and future trajectories has remained unclear. To address this gap, we applied a machine learning (ML) approach, integrating soil metagenomic data with environmental variables—soil properties, topography, vegetation, and climate—to predict the relative abundance of enzyme‐encoding genes for soil carbon (C), nitrogen (N), and phosphorus (P) across surface soils of the continental United States. We assessed potential responses under future emission scenarios (SSP2‐4.5 and SSP5‐8.5) by comparing a baseline (1985–2014) to a future period (2071–2100). The ML model explained 57%–63% of baseline variation. Precipitation was identified as the most influential factor for the relative abundance of C‐ and N‐degrading enzyme‐encoding genes, while slope length, representing horizontal distance that water can travel downslope, was the primary driver for P‐degrading enzyme‐encoding genes abundance. Projections revealed spatially heterogeneous shifts across continental US ecoregions: the relative abundance of C‐ and N‐degrading enzyme‐encoding genes decreased in wetter ecoregions and increased in drier ecoregions under future climate, while P‐degrading enzyme‐encoding genes abundance decreased significantly in semiarid and Mediterranean ecoregions. This study demonstrates the utility of metagenomic data for mapping soil genetic potential and predicting its regional response to environmental change, to inform ecosystem management strategies.

extracellular enzyme-encoding genes↗

Seeing the forest for all the trees: Mycorrhizal‐associated nutrient economies are modulated by stem density and the synchrony between overstorey and understorey communities

1. Identifying relationships between plant communities and soil characteristics is a critical step in understanding the consequences of species gains and losses in ecosystems. The mycorrhizal associated nutrient economy (MANE) hypothesis predicts that the degree to which tree species and their mycorrhizal associates affect soil properties is driven by the relative dominance of different mycorrhizal types (often measured by relative basal area). While this approach emphasizes the importance of canopy trees, it does not account for how other factors (e.g. the density and composition of understorey trees) may alter tree–soil relationships. 2. We analysed tree–soil data from an eastern deciduous forest in southern Indiana, USA that contains >29,000 georeferenced stems, including 21 species that associate with arbuscular mycorrhizal (AM) fungi and 14 that associate with ectomycorrhizal (ECM) fungi. We sampled soils (upper 5 cm) from across the plot and modelled soil characteristics to tree communities. Given differences in soil characteristics among AM-dominated and ECM-dominated neighbourhoods, we hypothesized that relationships between tree-mycorrhizal dominance and soils would be affected by both the density of stems in the plot (owing to ‘Zinke’ individual plant effects) and composition of the understorey trees (owing to ‘trait divergence effects’) 3. In support of our Zinke hypothesis, we found that the relationships between tree-mycorrhizal dominance and soil variables (soil pH, nitrification rates and carbon to nitrogen ratio) were strengthened as stand density increased. In support of the trait divergence hypothesis, we found that as the mismatch between overstorey and understorey composition increased (e.g. AM-dominated understories beneath ECM-dominated overstories and vice versa), the relationship between tree dominance and soil variables weakened. We were able to use these insights to create mycorrhizal metrics to predict soil variables that were sensitive to the structural composition of neighbourhoods. 4. Synthesis. Our results indicate that relationships between plants and soils in forests are not only shaped by dominant overstorey trees but also the density and composition of understorey trees. Thus, efforts to predict the ecosystem consequences of species gains and losses may benefit from considering these elements of forest structure and not only the basal area of the dominant trees.

MANE framework↗