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At least 145 records · Page 8

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NASA NTRS2011

Structure of 2,4-Diaminopyrimidine - Theobromine Alternate Base Pairs

We report the structure of clusters of 2,4-diaminopyrimidine with 3,7-dimethylxanthine (theobromine) in the gas phase determined by IR-UV double resonance spectroscopy in both the near-IR and mid-IR regions in combination with ab initio computations. These clusters represent potential alternate nucleobase pairs, geometrically equivalent to guanine-cytosine. We have found the four lowest energy structures, which include the Watson-Crick base pairing motif. This Watson-Crick structure has not been observed by resonant two-photon ionization (R2PI) in the gas phase for the canonical DNA base pairs.

Gengeliczki, Zsolt↗

NASA NTRS2012

Dig Hazard Assessment Using a Stereo Pair of Cameras

This software evaluates the terrain within reach of a lander s robotic arm for dig hazards using a stereo pair of cameras that are part of the lander s sensor system. A relative level of risk is calculated for a set of dig sectors. There are two versions of this software; one is designed to run onboard a lander as part of the flight software, and the other runs on a PC under Linux as a ground tool that produces the same results generated on the lander, given stereo images acquired by the lander and downlinked to Earth. Onboard dig hazard assessment is accomplished by executing a workspace panorama command sequence. This sequence acquires a set of stereo pairs of images of the terrain the arm can reach, generates a set of candidate dig sectors, and assesses the dig hazard of each candidate dig sector. The 3D perimeter points of candidate dig sectors are generated using configurable parameters. A 3D reconstruction of the terrain in front of the lander is generated using a set of stereo images acquired from the mast cameras. The 3D reconstruction is used to evaluate the dig goodness of each candidate dig sector based on a set of eight metrics. The eight metrics are: 1. The maximum change in elevation in each sector, 2. The elevation standard deviation in each sector, 3. The forward tilt of each sector with respect to the payload frame, 4. The side tilt of each sector with respect to the payload frame, 5. The maximum size of missing data regions in each sector, 6. The percentage of a sector that has missing data, 7. The roughness of each sector, and 8. Monochrome intensity standard deviation of each sector. Each of the eight metrics forms a goodness image layer where the goodness value of each sector ranges from 0 to 1. Goodness values of 0 and 1 correspond to high and low risk, respectively. For each dig sector, the eight goodness values are merged by selecting the lowest one. Including the merged goodness image layer, there are nine goodness image layers for each stereo pair of mast images.

Rankin, Arturo L.↗

NASA NTRS2014

Analysis and Simulation of the Simplified Aircraft-Based Paired Approach Concept With the ALAS Alerting Algorithm in Conjunction With Echelon and Offset Strategies

This report presents analytical and simulation results of an investigation into proposed operational concepts for closely spaced parallel runways, including the Simplified Aircraft-based Paired Approach (SAPA) with alerting and an escape maneuver, MITRE?s echelon spacing and no escape maneuver, and a hybrid concept aimed at lowering the visibility minima. We found that the SAPA procedure can be used at 950 ft separations or higher with next-generation avionics and that 1150 ft separations or higher is feasible with current-rule compliant ADS-B OUT. An additional 50 ft reduction in runway separation for the SAPA procedure is possible if different glideslopes are used. For the echelon concept we determined that current generation aircraft cannot conduct paired approaches on parallel paths using echelon spacing on runways less than 1400 ft apart and next-generation aircraft will not be able to conduct paired approach on runways less than 1050 ft apart. The hybrid concept added alerting and an escape maneuver starting 1 NM from the threshold when flying the echelon concept. This combination was found to be effective, but the probability of a collision can be seriously impacted if the turn component of the escape maneuver has to be disengaged near the ground (e.g. 300 ft or below) due to airport buildings and surrounding terrain. We also found that stabilizing the approach path in the straight-in segment was only possible if the merge point was at least 1.5 to 2 NM from the threshold unless the total system error can be sufficiently constrained on the offset path and final turn.

Torres-Pomales, Wilfredo↗

NASA NTRS2019

Pairing Relations of the CO3 Chondrites Recovered at the Dominion Range, Transantarctic Mountains

Fourteen CO3 chondrites have been recovered in the Dominion Range (DOM) dense collection area of the Transantarctic Mtns by ANSMET teams during the 2008-09, 2010-11, and 2014-15 seasons [1-3]. DOM 08006, one of the largest masses, has been studied extensively and is recognized to be a very primitive unmetamor-phosed sample of great value to planetary science [4]. Studies of pre-solar grains, organics, chondrules, inclusions, and matrix have revealed a rich scientific treasure helping to constrain the conditions in the early solar system (e.g., [4-8]). Many of the masses paired with this sample are significant, yet the pairing has been called into question due to the finding that DOM 08004 seems less primitive than DOM 08006 [5]. Because of the significant masses involved, and the great scientific value of DOM 08006, we have undertaken a detailed assessment of the pairing using field relations, macroscopic observations, petrography, and olivine compositions.

Righter, K.↗

NASA NTRS2003

Optimization of single-base-pair mismatch discrimination in oligonucleotide microarrays

The discrimination between perfect-match and single-base-pair-mismatched nucleic acid duplexes was investigated by using oligonucleotide DNA microarrays and nonequilibrium dissociation rates (melting profiles). DNA and RNA versions of two synthetic targets corresponding to the 16S rRNA sequences of Staphylococcus epidermidis (38 nucleotides) and Nitrosomonas eutropha (39 nucleotides) were hybridized to perfect-match probes (18-mer and 19-mer) and to a set of probes having all possible single-base-pair mismatches. The melting profiles of all probe-target duplexes were determined in parallel by using an imposed temperature step gradient. We derived an optimum wash temperature for each probe and target by using a simple formula to calculate a discrimination index for each temperature of the step gradient. This optimum corresponded to the output of an independent analysis using a customized neural network program. These results together provide an experimental and analytical framework for optimizing mismatch discrimination among all probes on a DNA microarray.

NASA Discipline Life Sciences Technologies↗

NASA NTRS2002

Single-base-pair discrimination of terminal mismatches by using oligonucleotide microarrays and neural network analyses

The effects of single-base-pair near-terminal and terminal mismatches on the dissociation temperature (T(d)) and signal intensity of short DNA duplexes were determined by using oligonucleotide microarrays and neural network (NN) analyses. Two perfect-match probes and 29 probes having a single-base-pair mismatch at positions 1 to 5 from the 5' terminus of the probe were designed to target one of two short sequences representing 16S rRNA. Nonequilibrium dissociation rates (i.e., melting profiles) of all probe-target duplexes were determined simultaneously. Analysis of variance revealed that position of the mismatch, type of mismatch, and formamide concentration significantly affected the T(d) and signal intensity. Increasing the concentration of formamide in the washing buffer decreased the T(d) and signal intensity, and it decreased the variability of the signal. Although T(d)s of probe-target duplexes with mismatches in the first or second position were not significantly different from one another, duplexes with mismatches in the third to fifth positions had significantly lower T(d)s than those with mismatches in the first or second position. The trained NNs predicted the T(d) with high accuracies (R(2) = 0.93). However, the NNs predicted the signal intensity only moderately accurately (R(2) = 0.67), presumably due to increased noise in the signal intensity at low formamide concentrations. Sensitivity analysis revealed that the concentration of formamide explained most (75%) of the variability in T(d)s, followed by position of the mismatch (19%) and type of mismatch (6%). The results suggest that position of the mismatch at or near the 5' terminus plays a greater role in determining the T(d) and signal intensity of duplexes than the type of mismatch.

Non-NASA Center↗

NASA NTRS1972

Real-time pair-feeding of animals

Automatic pair-feeding system was developed which immediately dispenses same amount of food to control animal as has been consumed by experimental animal that has free access to food. System consists of: master feeding system; slave feeding station; and control mechanism. Technique performs real time pair-feeding without attendant time lag.

Leon, H. A.↗

NASA NTRS1973

High-Q bandpass resonators utilizing bandstop resonator pairs

A high-Q bandpass resonators utilizing composite bandstop resonator pairs is reported. The bandstop resonator pairs are formed of composite series or parallel connected realizable transmission line elements. The elements are exclusively either quarter-wavelength lines or half-wavelength lines.

Okean, H. C.↗

NASA NTRS1973

Digital processing of stereoscopic image pairs.

The problem under consideration is concerned with scene analysis during robot navigation on the surface of Mars. In this mode, the world model of the robot must be continuously updated to include sightings of new obstacles and scientific samples. In order to describe the content of a particular scene, it is first necessary to segment it into known objects. One technique for accomplishing this segmentation is by analyzing the pair of images produced by the stereoscopic cameras mounted on the robot. A heuristic method is presented for determining the range for each point in the two-dimensional scene under consideration. The method is conceptually based on a comparison of corresponding points in the left and right images of the stereo pair. However, various heuristics which are adaptive in nature are used to make the algorithm both efficient and accurate. Examples are given of the use of this so-called range picture for the purpose of scene segmentation.

Levine, M. D.↗

NASA NTRS1974

Automatic real-time pair-feeding system for animals

A pair feeding method and apparatus are provided for experimental animals wherein the amount of food consumed is immediately delivered to a normal or control animal so that there is a qualitative, quantitative and chronological correctness in the pair feeding of the two animals. This feeding mechanism delivers precisely measured amounts of food to a feeder. Circuitry is provided between master and slave feeders so that there is virtually no chance of a malfunction of the feeding apparatus, causing erratic results. Recording equipment is also provided so that an hourly record is kept of food delivery.

Leon, H. A.↗

NASA NTRS1973

Hydrogen depolarized cell pair definition for space station application

Evaluation testing of the cell pair design of an electrochemical carbon dioxide collection subsystem was conducted. The system is proposed for use with the space station prototype. The objectives of the analytical and miscellaneous tasks in support of the test program are explained. An analysis was made of the number of cells required for the space station prototype. It was determined that 33 cell pairs would satisfy the space station prototype performance.

Russell, C. R.↗

NASA NTRS1974

Character of the pair interaction in solid and gaseous H2

Existing theoretical H2 pair potentials are investigated. We develop a spherically symmetric potential applicable to solid H2. It yields a ground-state energy of -98.7 K (experimental values range from -93.5 to -101 K). Pressure-volume data are also in excellent agreement with experiment between 0 and 25 kbar. Second virial coefficients using this potential are consistently too low. When an anisotropic pair potential is used, agreement becomes very good.

England, W.↗

NASA NTRS1974

The effects of simultaneous but unequal response-independent pay to pairs of human subjects on masseter EMG and bodily movements

Electromyographic activity and bodily movement of the masseter muscle were recorded in three pairs of human subjects, where one member of each pair was systematically presented with greater pay and each could reduce the value of money received by the other. The number of biting responses was as high or higher for the subject receiving less money immediately after coin delivery. However, the number of masseter contractions for the subject receiving more money remained higher at other times during the unequal pay conditions. No responses of pay reduction were emitted by any subject toward another.

Keenan, D. M.↗

NASA NTRS1975

A Model for the Vortex Pair Associated with a Jet in a Cross Flow

A model is presented for the contrarotating vortex pair that is formed by a round, turbulent, subsonic jet directed normally into a uniform, subsonic cross flow. The model consists of a set of algebraic equations that describe the properties of the vortex pair as a function of their location in the jet plume. The parameters of the model are physical characteristics of the vortices such as the vortex strength, spacing, and core size. These parameters are determined by velocity measurements at selective points in the jet plume.

Sellers, William L.↗

NASA NTRS1974

Simultaneous master-slave Omega pairs

Master-slave sequence ordering of the Omega system is suggested as a method of improving the pair geometry for low-cost receiver user benefit. The sequence change will not affect present sophisticated processor users other than require new labels for some pair combinations, but may require worldwide transmitter operators to slightly alter their long-range synchronizing techniques.

Burhans, R. W.↗

NASA NTRS1975

Measurements of selected psi /3684/ branching ratios from a study of secondary lepton pairs

The measurements considered were made at the electron-positron storage ring at the Stanford Linear Accelerator Center. The apparatus used consists of two identical spectrometers mounted in a collinear configuration about the beam-interaction region. The energies of secondary electrons or positrons are measured with good resolution in large NaI(Tl) total-absorption detectors. Data accumulated without gamma-ray converters were examined for electron-positron pairs. The data taken with gamma-ray converters in place and at center-of-mass energies within 1 MeV of the resonance energy were scanned for evidence of either charged particles or converted gamma rays accompanying the detected muon pairs.

Hilger, E.↗

NASA NTRS1975

Tailoff thrust and impulse imbalance between pairs of Space Shuttle solid rocket motors

The tailoff thrust and impulse imbalance between pairs of solid rocket motors is of particular interest for the Space Shuttle Vehicle because of the potential control problems that exist with this asymmetric configuration. Although a similar arrangement of solid rocket motors was utilized for the Titan Program, they produced less than one-half the thrust level of the Space Shuttle at web action time, and the overall vehicle was symmetric. Since the Titan Program does provide the most applicable actual test data, 23 flight pairs were analyzed to determine the actual tailoff thrust and impulse imbalance experienced. The results were scaled up using the predicted web action time thrust and tailoff time to arrive at values for the Space Shuttle. These values were then statistically treated to obtain a prediction of the maximum imbalance one could expect to experience during the Shuttle Program.

Jacobs, E. P.↗

NASA NTRS1976

A system for extracting 3-dimensional measurements from a stereo pair of TV cameras

Obtaining accurate three-dimensional (3-D) measurement from a stereo pair of TV cameras is a task requiring camera modeling, calibration, and the matching of the two images of a real 3-D point on the two TV pictures. A system which models and calibrates the cameras and pairs the two images of a real-world point in the two pictures, either manually or automatically, was implemented. This system is operating and provides three-dimensional measurements resolution of + or - mm at distances of about 2 m.

Yakimovsky, Y.↗