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At least 145 records · Page 8

Fatigue crack initiation and small crack growth in several airframe alloys

The growth of naturally-initiated small cracks under a variety of constant amplitude and variable amplitude load sequences is examined for several airframe materials: the conventional aluminum alloys, 2024-T3 and 7075-T6, the aluminum-lithium alloy, 2090-T8E41, and 4340 steel. Loading conditions investigated include constant amplitude loading at R = 0.5, 0, -1 and -2 and the variable amplitude sequences FALSTAFF, Mini-TWIST and FELIX/28. Crack growth was measured at the root of semicircular edge notches using acetate replicas. Crack growth rates are compared on a stress intensity factor basis, to those for large cracks to evaluate the extent of the small crack effect in each alloy. In addition, the various alloys are compared on a crack initiation and crack growth morphology basis.

Swain, M. H.↗

Fatigue crack initiation and small crack growth in several airframe alloys

The growth of naturally-initiated small cracks under a variety of constant amplitude and variable amplitude load sequences is examined for several airframe materials: the conventional aluminum alloys, 2024-T3 and 7075-T6, the aluminum-lithium alloy, 2090-T8E41 and 4340 steel. Loading conditions investigated include constant amplitude loading at R = 0.5, 0, -1 and -2 and the variable amplitude sequences FALSTAFF, Mini-TWIST, and FELIX/28. Crack growth was measured at the root of semicircular edge notches using acetate replicas. Crack growth rates are compared on a stress intensity factor basis, to those for large cracks to evaluate the extent of the small crack effect in each alloy. In addition, the various alloys are compared on a crack initiation and crack growth morphology basis.

Swain, M. H.↗

The microtubule cytoskeleton does not integrate auxin transport and gravitropism in maize roots

The Cholodny-Went hypothesis of gravitropism suggests that the graviresponse is controlled by the distribution of auxin. However, the mechanism of auxin transport during the graviresponse of roots is still unresolved. To determine whether the microtubule (MT) cytoskeleton is participating in auxin transport, the cytoskeleton was examined and the movement of 3H-IAA measured in intact and excised taxol, oryzalin, and naphthylphthalamic acid (NPA)-treated roots of Zea mays cv. Merit. Taxol and oryzalin did not inhibit the graviresponse of roots but the auxin transport inhibitor NPA greatly inhibited both auxin transport and graviresponse. NPA had no effect on MT organization in vertical roots, but caused MT reorientation in horizontally placed roots. Regardless of treatment, the organization of MTs in intact roots differed from that in root segments. The MT inhibitors, taxol and oryzalin had opposite effects on the MTs, namely, depolymerization (oryzalin) and stabilization and thickening (taxol), but both treatments caused swelling of the roots. The data indicate that the MT cytoskeleton does not directly interfere with auxin transport or auxin-mediated growth responses in maize roots.

NASA Discipline Plant Biology↗

Passive Water Assurance Delivery System

This report provides an analysis and evaluation of proposed design improvements to the internal water delivery system of NASA’s passive vegetable growing system. The VEGGIE system is designed to provide fresh vegetables and psychological benefits for astronauts aboard the International Space Station. Preliminary testing conducted by NASA for the VEGGIE system revealed flaws in the water delivery system. In these tests, the interface between the plants and the water reservoir failed to provide passive water delivery through the system both in microgravity experiments and experiments conducted on Earth. Our team’s goal was to fix this interface such that water can be passively delivered from the water reservoir to the plant rooting pillows to grow vegetative crops from seed to harvest. The system requirements outlined by NASA include: minimal total mass, on-demand passive water delivery, same dimensions as existing fixtures, minimal pressure on system to prevent leaking, zero mold growth, minimal swelling or clogging for non-flammable capillary materials, pressure stabilization between the water reservoir and plant pillow bags, even dispersal of water during initial priming, minimal bubble obstruction of capillary interface, maximum gas availability to plant roots, growth in a mixed artificial media (50:50 Arcillite:Fafard #2), and to avoid overwatering and drought conditions for plants. The major proposed design improvements are to use a single-interface capillary cord design to directly connect the water reservoir to the plant-rooting pillows, and to alter the water reservoir to model a propellant management device (PMD) in order to ensure consistent and long term watering for the VEGGIE system. The plant rooting pillow required minimal changes outside of replacing the capillary mat on the bottom of the pillow with O-ring insertion points the single-interface capillary system. - Research was conducted to determine which materials are able to uptake water through capillary action to grow Outredgeous Lettuce plants from seed to harvest. The primary requirements to be met by the team's design and evaluation of the capillary interface were: continuous passive watering for 90 days using non-flammable capillary materials and a peak water delivery rate of 30 mL/hr./0.15 m 2 . The results of the team's experimentation showed that the capillary material Nomex displayed the highest capillary water delivery potential with a maximum flow rate of 3.6 mL/hr. The Nomex capillary systems were the only capillary material to consistently grow Outredgeous Lettuce plants from seed to harvest, and displayed the highest average flow rate for multiple experiment sets. The Nomex material was previously incorporated into the VEGGIE system using a matted version of the capillary material, though the team recommends using a cord configuration in the single-interface capillary system for greater system stability. Nomex has proven to be a promising material, as it has passed both health and fire standards for use aboard the International Space Station. PMDs are made of materials that utilize surface tension and adhesive forces to overcome adverse accelerations to improve stability and ensure fluid delivery. PMDs are typically used in fuel tanks to ensure fuel delivery. The team recommends using a sponge PMD in order to mitigate bubble obstruction, decrease system weight, and ensure reliable water delivery to the capillary interface. The PMD water reservoir requires a rigid water reservoir and a vent tube for pressure stabilization. Since PMD’s cannot be tested in 1-G (Earth conditions), further theoretical modeling and testing is required for the proposed water reservoir design. The intent of this proposed system is to passively water plants in microgravity, though the technology is not limited to microgravity applications. The testing at The Ohio State University has proven that the design is highly effective on Earth, demonstrating that it could serve as a simple water delivery system in home and office applications. This would make vegetative crops more accessible in all indoor applications, thereby improving indoor air quality and occupant comfort. Additionally, this technology has great potential to be utilized in greenhouse plant production, cutting back on more sophisticated watering system energy and time requirements.

Alexandria Jensen↗

Chemical restrictions of roots in Ultisol subsoils lessened by long-term management

Exchangeable Al in subsoils of Ultisols in the southeastern USA can restrict rooting depth. Downward movement of basic cations (Ca, Mg, and K), applied as lime and fertilizer, may diminish that restriction over time. Materials from the argillic horizon were collected from three paired sites, having managed (long-term cropping) and nonmanaged topsoils (Typic Paleudults and Hapludults). One managed site was cropped continuously for 15 yr while the others were cultivated for more than 30 yr. Concentrations of extractable cations and other nutrients from the paired sites were compared to determine the magnitude of change due to management. The ability of the subsoils to support plant growth was evaluated in a missing-nutrient greenhouse experiment with sorghum [Sorghum bicolor (L.) Moench]. Subsoils of managed sites had greater effective cation-exchange capacity (CEC) and base saturation than those of non-managed sites. While availabilities of Ca, Mg, and K in subsoils of nonmanaged sites were inadequate to support maximal plant growth, they were adequate in subsoils of managed sites. Compared with nonmanaged sites, KCl-exchangeable Al in subsoils of managed sites was 23% lower at the 15-yr location and 65 and 100% lower at the two other locations. In the absence of lime, sorghum growth was almost totally inhibited on nonmanaged subsoils amended with optimum nutrients. On the managed subsoils, where 100, 65, and 23% of the nonmanaged exchangeable Al had been neutralized by topsoil fertilization and liming, growth reductions under the same conditions were 0, 50, and 100%, respectively. Thus, relatively long-term management had improved these Ultisol subsoils for root growth and development.

NASA Discipline Life Support Systems↗

Root cytoskeleton: its role in perception of and response to gravity

We have critically evaluated the possible functions of the plant cytoskeleton in root gravisensing and graviresponse and discussed the evidence that microtubules (MTs) and actin microfilaments (MFs) do not control differential cell growth during bending of roots. On the other hand, MF and MT networks are envisaged to participate in gravisensing because of the mechanical properties of the cytoskeletal structures that interconnect plant cell organelles with the plasma membrane. In restrained gravisensing, forces are suggested to be transmitted to membranes because large-scale gravity-dependent repositioning of organelles is effectively prevented due to the cytoskeleton-mediated anchorage of their envelopes at the plasma membrane. From the cytoskeletal point of view, we can also envisage an unrestrained gravity sensing when cytoskeletal tethers are not strong enough to preserve the tight control over distribution of organelles and the latter, if heavy enough, are allowed to sediment towards the physical bottom of cells. This situation obviously occurs in root cap statocytes because these uniquely organized cells are depleted of prominent actin MF bundles, endoplasmic MT arrays, and ER elements in their internal cytoplasm. Nevertheless, indirect evidence clearly indicates that sedimented root cap statoliths are enmeshed within fine but dynamic MF networks and that their behaviour is obviously under, at least partial, cytoskeletal control. The actomyosin-enriched domain among and around amyloplasts is proposed to increase the perception of gravity due to the grouping effect of sedimenting statoliths. Cytoskeletal links between myosin-rich statoliths, and cell peripheries well equipped with dense cortical MTs, membrane-associated cytoskeleton, as well as with ER elements, would allow efficient restrained gravisensing only at the statocyte cell cortex. As a consequence of cytoskeletal depletion in the internal statocyte cytoplasm and bulk sedimentation of large amyloplasts, restrained gravisensing is spatially restricted to the bottom of the statocyte irrespective of whether roots are vertical or horizontal. This spatial aspect allows for efficient gravisensing via amplification of gravity-induced impacts on the cellular architecture, a phenomenon which is unique to root cap statocytes.

NASA Discipline Plant Biology↗

Investigation of VEGGIE Root Mat

VEGGIE is a plant growth facility that utilizes the phenomenon of capillary action as its primary watering system. A cloth made of Meta Aramid fiber, known as Nomex is used to wick water up from a reservoir to the bottom of the plants roots. This root mat system is intended to be low maintenance with no moving parts and requires minimal crew interface time. Unfortunately, the water wicking rates are inconsistent throughout the plant life cycle, thus causing plants to die. Over-wicking of water occurs toward the beginning of the cycle, while under-wicking occurs toward the middle. This inconsistency of wicking has become a major issue, drastically inhibiting plant growth. The primary objective is to determine the root cause of the inconsistent wicking through experimental testing. Suspect causes for the capillary water column to break include: a vacuum effect due to a negative pressure gradient in the water reservoir, contamination of material due to minerals in water and back wash from plant fertilizer, induced air bubbles while using syringe refill method, and material limitations of Nomex's ability to absorb and retain water. Experimental testing will be conducted to systematically determine the cause of under and over-wicking. Pressure gages will be used to determine pressure drop during the course of the plant life cycle and during the water refill process. A debubbler device will be connected to a root mat in order to equalize pressure inside the reservoir. Moisture and evaporation tests will simultaneously be implemented to observe moisture content and wicking rates over the course of a plant cycle. Water retention tests will be performed using strips of Nomex to determine materials wicking rates, porosity, and absorptivity. Through these experimental tests, we will have a better understanding of material properties of Nomex, as well as determine the root cause of water column breakage. With consistent test results, a forward plan can be achieved to resolve the issue and give valuable insight for the next generation of VEGGIE.

Subbiah, Arun M.↗

Early Prototype Development of the Passive Orbital Nutrient Delivery System (PONDS)

Long-duration space missions will eventually require a fresh food supply to supplement crew diets, which means growing crops in space. The Passive Orbital Nutrient Delivery System (PONDS) is a new plant growth approach that contains both an area for a contained substrate and a reservoir for water and/or plant nutrient solutions. Ground studies have shown that the system facilitates both reliable water delivery to seeds for germination (e.g., while avoiding overwatering), and transport of water from the reservoir for improved plant growth while providing nutrients and oxygen to the root zone. In ground prototypes a capillary mat wicking material passively links the water/nutrient solution reservoir to a removable rooting module containing a substrate adapted to support plant growth. Oxygen permeable membranes are incorporated into both the reservoir walls and the rooting modules, bringing in oxygen from outside of the system into the reservoir and then into the rooting modules where the plant roots proliferate. Water is delivered from the reservoir to the substrate contained within the rooting module through the use of wicking material inserted into the plant growth substrate both from the bottom and from the sides of the rooting module. The capillary mat material is intrinsically hydrophilic and continuously wicks water to the substrate throughout the plant growth interval. The system is therefore self-watering in terms of supplying water to the root zone encompassed within the rooting module on demand. At the top, a hydrophilic phenolic foam plug surrounds the wick in the seed insertion zone, and both contains the substrate within the rooting module, and facilitates removal of excess moisture from the capillary mat wick before it can encompass seeds prior to germination. This work is supported by NASA’s Space Life and Physical Sciences and Research Applications Division (SLPSRAD).

Levine, Howard G.↗

Three-Dimensional Gear Crack Propagation Studies

Three-dimensional crack growth simulation was performed on a split-tooth gear design using boundary element modeling and linear elastic fracture mechanics. Initial cracks in the fillet of the teeth produced stress intensity factors of greater magnitude (and thus, greater crack growth rates) than those in the root or groove areas of the teeth. Crack growth simulation was performed on a case study to evaluate crack propagation paths. Tooth fracture was predicted from the crack growth simulation for an initial crack in the tooth fillet region. Tooth loads on the uncracked mesh of the split-tooth design were up to five times greater than those on the cracked mesh if equal deflections of the cracked and uncracked teeth were considered. Predicted crack shapes as well as crack propagation life are presented based on calculated stress intensity factors, mixed-mode crack propagation trajectory theories, and fatigue crack growth theories.

Lewicki, David G.↗

Inducing gravitropic curvature of primary roots of Zea mays cv Ageotropic

Primary roots of the mutant 'Ageotropic' cultivar of Zea mays are nonresponsive to gravity. Their root caps secrete little or no mucilage and touch the root only at the extreme apex. A gap separates the cap and root at the periphery of the cap. Applying mucilage from normal roots or substances with a consistency similar to that of mucilage to tips of mutant roots causes these roots to become strongly graviresponsive. Gravicurvature stops when these substances are removed. Caps of some mutants secrete small amounts of mucilage and are graviresponsive. These results indicate that (a) the lack of graviresponsiveness in the mutant results from disrupting the transport pathway between the cap and root, (b) movement of the growth-modifying signal from the cap to the root occurs via an apoplastic pathway, and (c) mucilage is necessary for normal communication between the root cap and root in Zea mays cv Ageotropic.

NASA Discipline Number 29-20↗

Three-Dimensional Gear Crack Propagation Studied

Gears used in current helicopters and turboprops are designed for light weight, high margins of safety, and high reliability. However, unexpected gear failures may occur even with adequate tooth design. To design an extremely safe system, the designer must ask and address the question, "What happens when a failure occurs?" With gear-tooth bending fatigue, tooth or rim fractures may occur. A crack that propagates through a rim will be catastrophic, leading to disengagement of the rotor or propeller, loss of an aircraft, and possible fatalities. This failure mode should be avoided. A crack that propagates through a tooth may or may not be catastrophic, depending on the design and operating conditions. Also, early warning of this failure mode may be possible because of advances in modern diagnostic systems. One concept proposed to address bending fatigue fracture from a safety aspect is a splittooth gear design. The prime objective of this design would be to control crack propagation in a desired direction such that at least half of the tooth would remain operational should a bending failure occur. A study at the NASA Lewis Research Center analytically validated the crack-propagation failsafe characteristics of a split-tooth gear. It used a specially developed three-dimensional crack analysis program that was based on boundary element modeling and principles of linear elastic fracture mechanics. Crack shapes as well as the crack-propagation life were predicted on the basis of the calculated stress intensity factors, mixed-mode crack-propagation trajectory theories, and fatigue crack-growth theories. The preceding figures show the effect of the location of initial cracks on crack propagation. Initial cracks in the fillet of the teeth produced stress intensity factors of greater magnitude (and thus, greater crack growth rates) than those in the root or groove areas of the teeth. Crack growth was simulated in a case study to evaluate crack-propagation paths. Tooth fracture was predicted from the crackgrowth simulation for an initial crack in the tooth fillet region. This was the desired failure mode for an ultrasafe design. Lastly, tooth loads on the uncracked mesh of the split-tooth design were up to five times greater than those on the cracked mesh if equal deflections of the cracked and uncracked teeth were considered. This effect needs to be considered in the design of a split-tooth configuration. This work was done in-house at Lewis in support of the National Rotorcraft Technology Center project, Ultra-Safe Gear Design, with the Boeing Defense and Space Group. The crack-propagation package was developed by the Cornell Fracture Group at Cornell University. The reported results, which are the initial findings of Lewis gear-crackpropagation research for the Rotorcraft Base Program, will be further investigated to develop generalized gear design guidelines.

Lewicki, David G.↗

Demonstration of A Long Duration Crop in the Advanced Plant Habitat Engineering Demonstration Unit: Key Factors to Consider Prior to Testing and Lessons Learned

The Advanced Plant Habitat (APH) is a fully enclosed, closed-loop, plant growth facility currently deployed on the International Space Station (ISS). It is equipped with complete environmental control (i.e.spectral qualityandlight intensity, temperature, relative humidity, and CO2),root module which containsa growth substrate(typically arcillite) and a porous tube based automated water delivery system. Two APH units, one ground unitidentical to the on-orbit versionand an Engineering Development Unit (EDU; a high fidelity test version), currently reside at Kennedy Space Center. Prior to conducting tests on-orbit, these APH ground units areutilized to conductbothScience Verification Tests(SVT; primarilyinthe EDU) and Experiment Verification Tests (EVT; primarily in the Ground Unit). In February 2020, a 122 day experiment was concluded in the APH EDU where four 'Numex Española Improved' pepper plants (Capsicum annuum) were successfully grown from seed to harvestyieldingover 50 fruit, demonstrating the capabilityand versatilityof this APH EDU system to support plant growth for long life cycle flowering/fruiting plants.

L. E. Spencer↗

Springback and diagravitropism in Merit corn roots

Dark-treated Merit corn (Zea mays L.) roots are diagravitropic and lose curvature upon withdrawal of the gravity stimulus (springback). Springback was not detected in a variety of corn that is orthogravitropic in the dark, nor in Merit roots in which tropistic response was enhanced either with red light or with abscisic acid. A possible interpretation is that springback may be associated with a weak growth response of diagravitropic roots.

Non-NASA Center↗

Mapping the functional roles of cap cells in the response of Arabidopsis primary roots to gravity

The cap is widely accepted to be the site of gravity sensing in roots because removal of the cap abolishes root curvature. Circumstantial evidence favors the columella cells as the gravisensory cells because amyloplasts (and often other cellular components) are polarized with respect to the gravity vector. However, there has been no functional confirmation of their role. To address this problem, we used laser ablation to remove defined cells in the cap of Arabidopsis primary roots and quantified the response of the roots to gravity using three parameters: time course of curvature, presentation time, and deviation from vertical growth. Ablation of the peripheral cap cells and tip cells did not alter root curvature. Ablation of the innermost columella cells caused the strongest inhibitory effect on root curvature without affecting growth rates. Many of these roots deviated significantly from vertical growth and had a presentation time 6-fold longer than the controls. Among the two inner columella stories, the central cells of story 2 contributed the most to root gravitropism. These cells also exhibited the largest amyloplast sedimentation velocities. Therefore, these results are consistent with the starch-statolith sedimentation hypothesis for gravity sensing.

Non-NASA Center↗

Density and composition of microorganisms during long-term (418 day) growth of potato using biologically reclaimed nutrients from inedible plant biomass

A study evaluating alternative methods for long term operation of biomass production systems was recently completed at the Kennedy Space Center (KSC). The 418-day study evaluated repeated batch versus mixed-aged production of potato grown on either standard 1/2-strength Hoagland's nutrient solution or solutions including nutrients recycled from inedible plant material. The long term effects of closure and recycling on microbial dynamics were evaluated by monitoring the microbial communities associated with various habitats within the plant growth system (i.e., plant roots, nutrient solution, biofilms within the hydroponic systems, atmosphere, and atmospheric condensate). Plate count methods were used to enumerate and characterize microorganisms. Microscopic staining methods were used to estunate total cell densities. The primary finding was that the density and composition of microbial communities associated with controlled environmental plant growth systems are stable during long term operation. Continuous production resulted in slightly greater stability. Nutrient recycling, despite the addition of soluble organic material from the waste processing system, did not significantly increase microbial density in any of the habitats.

NASA Center KSC↗

The kinetics of root gravitropism: dual motors and sensors

The Cholodny-Went theory of tropisms has served as a framework for investigation of root gravitropism for nearly three quarters of a century. Recent investigations using modern techniques have generated findings consistent with the classical theory, including confirmation of asymmetrical distribution of polar auxin transport carriers, molecular evidence for auxin asymmetry following gravistimulation, and generation of auxin response mutants with predictable lesions in gravitropism. Other results indicate that the classical model is inadequate to account for key features of root gravitropism. Initiation of curvature, for example, occurs outside the region of most rapid elongation and is driven by differential acceleration rather than differential inhibition of elongation. The evidence indicates that there are two motors driving root gravitropism, one of which appears not to be auxin regulated. We have recently developed technology that is capable of maintaining a constant angle of gravistimulation at any selected target region of a root while continuously monitoring growth and curvature kinetics. This review elaborates on the advantages of this new technology for analyzing gravitropism and describes applications of the technology that reveal (1) the existence of at least two phases to gravitropic motor output, even under conditions of constant stimulus input and (2) the existence of gravity sensing outside of the root cap. We propose a revised model of root gravitropism including dual sensors and dual motors interacting to accomplish root gravitropism, with only one of the systems linked to the classical Cholodny-Went theory.

Review↗

Variability of Phenology and Fluxes of Water and Carbon with Observed and Simulated Soil Moisture in the Ent Terrestrial Biosphere Model (Ent TBM Version 1.0.1.0.0)

The Ent Terrestrial Biosphere Model (Ent TBM) is a mixed-canopy dynamic global vegetation model developed specifically for coupling with land surface hydrology and general circulation models (GCMs). This study describes the leaf phenology submodel implemented in the Ent TBM version 1.0.1.0.0 coupled to the carbon allocation scheme of the Ecosystem Demography (ED) model. The phenology submodel adopts a combination of responses to temperature (growing degree days and frost hardening), soil moisture (linearity of stress with relative saturation) and radiation (light length). Growth of leaves, sapwood, fine roots, stem wood and coarse roots is updated on a daily basis. We evaluate the performance in reproducing observed leaf seasonal growth as well as water and carbon fluxes for four plant functional types at five Fluxnet sites, with both observed and prognostic hydrology, and observed and prognostic seasonal leaf area index. The phenology submodel is able to capture the timing and magnitude of leaf-out and senescence for temperate broadleaf deciduous forest (Harvard Forest and Morgan- Monroe State Forest, US), C3 annual grassland (Vaira Ranch, US) and California oak savanna (Tonzi Ranch, US). For evergreen needleleaf forest (Hyytiäla, Finland), the phenology submodel captures the effect of frost hardening of photosynthetic capacity on seasonal fluxes and leaf area. We address the importance of customizing parameter sets of vegetation soil moisture stress response to the particular land surface hydrology scheme. We identify model deficiencies that reveal important dynamics and parameter needs.

Soil moisture↗

Evaluating Differences Among Crop Models in Simulating Soybean in-Season Growth

Crop models are useful tools for simulating agricultural systems that require continued model development and testing to increase their robustness and improve how they describe our current understanding of processes. Coordinated and “blind” evaluation of multiple models using same protocols and experimental datasets provides unique opportunities to further improve models and enhance their reliability. For soybean [Glycine max (L.) Merr.], there has been limited coordinated multi-model evaluations for the simulation of in-season plant growth dynamics. We evaluated ten dynamic soybean crop models for their simulation of in-season plant growth using data from five experiments conducted in Argentina, Brazil, France, and USA. We evaluated models after a Blind (using only phenology data) and a Full calibration (with in-season and end-of-season variables). Calibration reduced model uncertainty by reducing standard bias for the simulation of in-season variables (biomass, leaf, pod, and stem weights, and leaf area index, LAI). However, we found that most models had difficulty in reproducing leaf growth dynamics, with normalized root mean squared error (nRMSE) of 56% for leaf weight and 43% for LAI (across locations and models after Full calibration). Models with different levels of complexity and experience were capable of simulating final seed yield at maturity with reasonable accuracy (nRMSE of 8–31% after Full calibration). However, the nRMSE for pod weight (of 17–64% after Full calibration) was two-fold larger than that of seed yield. Moreover, the models differed in how they simulated timing from sowing to beginning seed growth (47–93 days) and effective seed filling period (18–54 days), owing to model structural differences in defining the reproductive developmental stages. Overall, we identified the following processes that can benefit from further model improvement: leaf expansion and senescence, reproductive phenology, and partitioning to reproductive growth. Simulation of pod wall tissue and individual seed cohorts is another aspect that many models currently lack. Model improvement can benefit from high-temporal resolution experimental datasets that concurrently account for phenology, plant growth, and partitioning. Further, we recommend collecting reproductive phenology in the field consistent with actual dry matter allocation to organs in the models and collecting multiple observations of seed and pod weight to aid model improvement for simulation of seed growth and yield formation.

Agricultural Model Intercomparison and Improvement↗