Search NASA⌕ Search

SEARCH · Search NASA

Results for “Docking”

Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 1,441 records · Page 80
All sourcesNASA NTRSDOE OSTI
DOE OSTI2024

CryoEM structures of the human CLC-2 voltage-gated chloride channel reveal a ball-and-chain gating mechanism

CLC-2 is a voltage-gated chloride channel that contributes to electrical excitability and ion homeostasis in many different tissues. Among the nine mammalian CLC homologs, CLC-2 is uniquely activated by hyperpolarization, rather than depolarization, of the plasma membrane. The molecular basis for the divergence in polarity of voltage gating among closely related homologs has been a long-standing mystery, in part because few CLC channel structures are available. Here, we report cryoEM structures of human CLC-2 at 2.46 – 2.76 Å, in the presence and absence of the selective inhibitor AK-42. AK-42 binds within the extracellular entryway of the Cl – -permeation pathway, occupying a pocket previously proposed through computational docking studies. In the apo structure, we observed two distinct conformations involving rotation of one of the cytoplasmic C-terminal domains (CTDs). In the absence of CTD rotation, an intracellular N-terminal 15-residue hairpin peptide nestles against the TM domain to physically occlude the Cl – -permeation pathway. This peptide is highly conserved among species variants of CLC-2 but is not present in other CLC homologs. Previous studies suggested that the N-terminal domain of CLC-2 influences channel properties via a “ball-and-chain” gating mechanism, but conflicting data cast doubt on such a mechanism, and thus the structure of the N-terminal domain and its interaction with the channel has been uncertain. Through electrophysiological studies of an N-terminal deletion mutant lacking the 15-residue hairpin peptide, we support a model in which the N-terminal hairpin of CLC-2 stabilizes a closed state of the channel by blocking the cytoplasmic Cl – -permeation pathway.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2024

Novel sterol binding domains in bacteria

Sterol lipids are widely present in eukaryotes and play essential roles in signaling and modulating membrane fluidity. Although rare, some bacteria also produce sterols, but their function in bacteria is not known. Moreover, many more species, including pathogens and commensal microbes, acquire or modify sterols from eukaryotic hosts through poorly understood molecular mechanisms. The aerobic methanotroph Methylococcus capsulatus was the first bacterium shown to synthesize sterols, producing a mixture of C-4 methylated sterols that are distinct from those observed in eukaryotes. C-4 methylated sterols are synthesized in the cytosol and localized to the outer membrane, suggesting that a bacterial sterol transport machinery exists. Until now, the identity of such machinery remained a mystery. In this study, we identified three novel proteins that may be the first examples of transporters for bacterial sterol lipids. The proteins, which all belong to well-studied families of bacterial metabolite transporters, are predicted to reside in the inner membrane, periplasm, and outer membrane of M. capsulatus, and may work as a conduit to move modified sterols to the outer membrane. Quantitative analysis of ligand binding revealed their remarkable specificity for 4-methylsterols, and crystallographic structures coupled with docking and molecular dynamics simulations revealed the structural bases for substrate binding by two of the putative transporters. Their striking structural divergence from eukaryotic sterol transporters signals that they form a distinct sterol transport system within the bacterial domain. Finally, bioinformatics revealed the widespread presence of similar transporters in bacterial genomes, including in some pathogens that use host sterol lipids to construct their cell envelopes. The unique folds of these bacterial sterol binding proteins should now guide the discovery of other proteins that handle this essential metabolite.

59 BASIC BIOLOGICAL SCIENCES↗
NASA NTRS1967

Hydrostatic force used to handle outsized, heavy objects

Specially fitted barge is used to load and transport large, heavy objects to a dock side site. There the barge itself can lift, rotate, and position the objects. Typical functions are economically accomplished by water buoyancy.

Craft, G. W.↗
NASA NTRS1967

Astronautics and Aeronautics, 1966

At the opening of the tenth year in the era of man’s mobility in outer space, we can look back on 1966 as offering convincing evidence that the United States had gained great competence. This evidence included: five orbital space flights by ten Gemini astronauts; four lunar missions undertaking the orbiting of and soft landing on the moon; numerous contributions to scientific knowledge by unmanned spacecraft and sounding rockets; and further demonstrations of the practical utility of operational space systems, including weather and communications satellites. During 1966, a record 100 American spacecraft were placed into earth orbit or on escape trajectories. Thousands of revealing and useful pictures of the earth were taken from space and of the moon from lunar orbit and on its surface. The Gemini program ended with rendezvous and docking experiments and extravehicular activity by the Gemini test pilots as the Apollo R&D test flights leading to the manned lunar mission came into the schedule. Thirty-five major scientific, technological, and operational milestones were cited for 1966 by the President in his Report to the Congress on aeronautical and space activities of the United States. This was one measure of the American commitment to share in the peaceful exploration of space for all mankind. Another was our support of the final steps toward a United Nations space treaty, undertaken to ensure that the peaceful exploitation of space had juridical basis in international law. Spectacular as some events in the space venture were in 1966, they nonetheless came to have diminished novelty in the eyes of many laymen. The multitude of both important and unspectacular space activities attained almost an accepted and routine place. This volume, as well as its predecessor chronicles, offers a ready reference on the major as well as the less-well known events. Beyond the welter of documented details on the complex nature of aeronautical and space related events and their impact, this volume helps to provide a better perspective upon today as we contemplate tomorrow. Such intent also serves future historians and analysts who cannot be unmindful of what is herein presented. When the Congress created the National Aeronautics and Space Administration in 1958, it charged NASA with the responsibility to “contribute materially to . . . the expansion of human knowledge of phenomena in the atmosphere and space’’ and to “provide for the widest practical and appropriate dissemination of information concerning its activities and results thereof.” NASA has attempted to do this and to include documentation for the historical record. The relating of NASA history begins with this day-by day chronicle and leads to more specialized studies and histories of the unprecedented task of extending man’s mobility and understanding beyond his planet. While a chronology cannot in itself serve as a full-fledged history, the size of this annual volume alone is illustrative of the scope and complexity of the historical task yet to be completed as memories fade and the records disappear.

SPACE PROBE↗
NASA NTRS1966

Gemini status report.

Missions of Gemini VI through XI covering rendezvous, docking, thermal overloading, translation maneuver, etc

GEMINI PROJECT↗
NASA NTRS1968

Pressure variable orifice for hydraulic control valve

Hydraulic valve absorbs impact energy generated in docking or joining of two large bodies by controlling energy release to avoid jarring shock. The area of exit porting presented to the hydraulic control fluid is directly proportional to the pressure acting on the fluid.

Ammerman, R. L.↗
NASA NTRS1967

Gemini Summary Conference

The Gemini Summary Conference was held on February 1 and 2, 1967, at the NASA Manned Spacecraft Center, Houston, Tex. The conference emphasized the highlights of the Gemini Program and especially the flight results of the last five missions. This report contains the 21 technical papers presented at the conference as well as an introduction by George E. Mueller and concluding remarks by George M. Low. The technical papers are divided into five sections: the first describes the rendezvous, docking, and tethered-vehicle operations involving the spacecraft and a target vehicle; the second presents various aspects of extravehicular activity; the third concerns the operational support of the missions ; the fourth cover the experiments conducted during the missions; and the fifth compares the astronaut flight and simulation experiences and relates the Gemini results to the Apollo Program.

structural integrity↗
NASA NTRS1968

Program Completed

Orbital rendezvous and docking experiment of Cosmos 212 and 213 satellites

Source record↗