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All sourcesNASA NTRSDOE OSTI
NASA NTRS1997

Characterization of Multilayer Piezoelectric Actuators for Use in Active Isolation Mounts

Active mounts are desirable for isolating spacecraft science instruments from on-board vibrational sources such as motors and release mechanisms. Such active isolation mounts typically employ multilayer piezoelectric actuators to cancel these vibrational disturbances. The actuators selected for spacecraft systems must consume minimal power while exhibiting displacements of 5 to 10 micron under load. This report describes a study that compares the power consumption, displacement, and load characteristics of four commercially available multilayer piezoelectric actuators. The results of this study indicate that commercially available actuators exist that meet or exceed the design requirements used in spacecraft isolation mounts.

Wise, Stephanie A.↗
DOE OSTI2013

A NEW RESULT ON THE ORIGIN OF THE EXTRAGALACTIC GAMMA-RAY BACKGROUND

In this paper, we repeatedly use the method of image stacking to study the origin of the extragalactic gamma-ray background (EGB) at GeV bands, and find that the Faint Images of the Radio Sky at Twenty centimeters (FIRST) sources undetected by the Large Area Telescope on the Fermi Gamma-ray Space Telescope can contribute about (56 {+-} 6)% of the EGB. Because FIRST is a flux-limited sample of radio sources with incompleteness at the faint limit, we consider that point sources, including blazars, non-blazar active galactic nuclei, and starburst galaxies, could produce a much larger fraction of the EGB.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2022

Analysis of Orthogonal Efflux and Permeation Properties of Compounds Leads to the Discovery of New Efflux Pump Inhibitors

Optimization of compound permeation into Gram-negative bacteria is one of the most challenging tasks in the development of antibacterial agents. Two permeability barriers–the passive diffusion barrier of the outer membrane (OM) and active drug efflux–act synergistically to protect cells from the antibacterial action of compounds. In Escherichia coli (E. coli) and relatives, these two barriers sieve compounds based on different physicochemical properties that are defined by their interactions with OM porins and efflux pumps, respectively. In this study, we critically tested the hypothesis that the best substrates and inhibitors of efflux pumps are compounds that can effectively permeate the OM and are available at relatively high concentrations in the periplasm. For this purpose, we filtered a large subset of the ZINC15 database of commercially available compounds for compounds containing a primary amine, a chemical feature known to facilitate the uptake through E. coli general porins. The assembled library was screened by ensemble docking to AcrA, the periplasmic component of the AcrAB-TolC efflux pump, followed by experimental testing of the top predicted binders for antibacterial activities, efflux recognition, and inhibition. In this work, we found that the filtered primary amine library is a rich source of compounds with efflux-inhibiting activities and identified efflux pump inhibitors with novel chemical scaffolds effective against E. coli AcrAB-TolC and efflux pumps of multidrug-resistant clinical isolates of Acinetobacter baumannii. However, primary amines are not required for the recognition of compounds by efflux pumps and their efflux-inhibitory activities.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2012

VLBA DETERMINATION OF THE DISTANCE TO NEARBY STAR-FORMING REGIONS. V. DYNAMICAL MASS, DISTANCE, AND RADIO STRUCTURE OF V773 Tau A

We present multi-epoch Very Long Baseline Array (VLBA) observations of V773 Tau A, the 51 day binary subsystem in the multiple young stellar system V773 Tau. Combined with previous interferometric and radial velocity measurements, these new data enable us to improve the characterization of the physical orbit of the A subsystem. In particular, we infer updated dynamical masses for the primary and the secondary components of 1.55 {+-} 0.11 M{sub Sun} and 1.293 {+-} 0.068 M{sub Sun }, respectively, and an updated orbital parallax distance to the system of 135.7 {+-} 3.2 pc, all consistent with previous estimates. Using the improved orbit, we can calculate the absolute coordinates of the barycenter of V773 Tau A at each epoch of our VLBA observations, and fit for its trigonometric parallax and proper motion. This provides a direct measurement of the distance to the system almost entirely independent of the orbit modeling. The best fit yields a distance of 129.9 {+-} 3.2 pc, in good agreement (i.e., within 1{sigma}) with the distance estimate based on the orbital fit. Taking the mean value of the orbital and trigonometric parallaxes, we conclude that V773 Tau is located at d = 132.8 {+-} 2.3 pc. The accuracy of this determination is nearly one order of magnitude better than that of previous estimates. In projection, V773 Tau and two other young stars (Hubble 4 and HDE 283572) recently observed with the VLBA are located toward the dark cloud Lynds 1495, in the central region of Taurus. These three stars appear to have similar trigonometric parallaxes, radial velocities, and proper motions, and we argue that the weighted mean and dispersion of their distances (d = 131.4 pc and {sigma}{sub d} = 2.4 pc) provide a good estimate of the distance to and depth of Lynds 1495 and its associated stellar population. The radio emission from the two sources in V773 Tau A is largely of gyrosynchrotron origin. Interestingly, both sources are observed to become typically five times brighter near periastron than near apastron (presumably because of increased flaring activity), and the separation between the radio sources near periastron appears to be systematically smaller than the separation between the stars. While this clearly indicates some interaction between the individual magnetospheres, the exact mechanisms at play are unclear because even at periastron the separation between the stars ({approx}30 R{sub *}) remain much larger than the radius of the magnetospheres around these low-mass young stars ({approx}6 R{sub *}).

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2022

A Smart and Flexible Microgrid with a Low-cost Scalable Open-source Controller (Final Report)

This report contains information regarding the activities carried out in the project "A Smart and Flexible Microgrid with a Low-cost Scalable Open-source Controller. The project aims at developing a community-based flexible microgrid (FMG) with smart grid features, including multiple utility feeders and dynamic boundaries that utilize intelligent switches and ultra-high-speed communication links already in a smart grid. The project also aims at developing a corresponding controller for such an FMG with low cost and high scalability. With distributed renewable energy resources (DERs) and the intelligent microgrid controller, the FMG will achieve aggressive emission reduction, increased energy use efficiency, and reliability improvement goals. The FMG and its controller design will be scalable for different geographic areas, load sizes, distributed generation source number and types, and even multiple MGs within a distribution electric power system. In order to achieve these project objectives, three main development tasks were carried out: FMG design, FMG controller development, and FMG controller testing. The fourth task was technology to market.

24 POWER TRANSMISSION AND DISTRIBUTION↗
NASA NTRS1992

New inertial actuator provides isolation and stabilization in microgravity conditions

Experiments in materials and fluids processing have been conducted, or are planned, that take advantage of the low-g environment offered by space-based platforms. While the specific goals of the experiments vary, a common objective is to see improved results from the processes over those that would be obtained from similar ground-based experiments. While the space-based processing environment does offer a low-g environment, it is not disturbance free. Results of experiments already conducted, particularly those in manned vehicles, show that spacecraft induced disturbances still limit what can be achieved in materials processing. The duration of actual micro-g level environments is shorter than desired and periods of milli-g activity levels are not uncommon. While small scale experiments can be configured to overcome some of the vehicle disturbance sources, larger scale processing devices and commercial activities will require alternative methods to reduce the influences of spacecraft vibrations. Applied Technology Associates, Inc., (ATA), in cooperation with NASA Lewis Research Center, is developing hardware to provide a sustained micro-g experiment environment. This work is being sponsored under a Small Business Innovation Research (SBIR) program, which is currently in the Phase 2 development stage. ATA's approach is based on an inertial actuator, which when used as part of a closed-loop stabilization system, rejects unwanted disturbances to the experiment package. A prototype of the actuator has been fabricated and used in a laboratory demonstration to prove the principle of operation. ATA's presentation emphasized the development and testing of the Digital Materials Processing Experiment (DAMPER) inertial actuator. Physical and performance characteristics of the device are presented. Technical issues, including further optimization of the actuator's performance and plans for additional laboratory experiments, are also covered.

Blackburn, John↗
DOE OSTI2024

Multiwavelength investigations of PKS 2300–18: S-shaped radio quasar with precessing jets and double-peaked broad emission-line spectrum

ABSTRACT S-shaped radio galaxy jets are prime sources for investigating the dynamic interplay between the central active galactic nucleus (AGN), the jets, and the ambient intergalactic medium. These sources are excellent candidates for studying jet precession, as their S-shaped inversion symmetry strongly indicates underlying precession. We present a multiwavelength analysis of the giant inversion-symmetric S-shaped radio galaxy PKS 2300$-$18, which spans 0.76 Mpc. The host is a quasar at a redshift of 0.128, displaying disturbed optical morphology due to an ongoing merger with a companion galaxy. We conducted a broad-band radio spectral study using multifrequency data ranging from 183 MHz to 6 GHz, incorporating dedicated observations with the upgraded Giant Metrewave Radio Telescope (uGMRT) and Karl G. Jansky Very Large Array (JVLA) alongside archival radio data. A particle injection model was fitted to the spectra of different regions of the source to perform ageing analysis, which was supplemented with a kinematic jet precession model. The ageing analysis revealed a maximum plasma age of $\sim$ 40 Myr, while the jet precession model indicated a precession period of $\sim$ 12 Myr. ROentgen SATellite (ROSAT) data revealed an X-ray halo of Mpc size, and from Chandra the AGN X-ray spectrum was modelled using thermal and power-law components. The optical spectrum displaying double-peaked broad emission lines was modelled, indicating complex broad-line region kinematics at the centre with the possibility of a binary SMBH. We present the results of our multiwavelength analysis of the source, spanning scales from a few light-days to a few Mpc, and discuss its potential evolutionary path.

Misra, Arpita↗
DOE OSTI2016

A robust derivation of the tight relationship of radio core dominance to inclination angle in high redshift 3CRR sources

It is believed that, in radio-loud active galactic nuclei (AGNs), the core radio flux density can be normalized to the flux density of the extended lobe emission to infer the orientation of a radio source. However, very little is known about the reliability and precision of this method, and we are unaware of any robust conversion recipe to infer the inclination from the core dominance. Investigating whether or not the radio core dominance parameter R separates the quasars from the radio galaxies in the z ⩾ 1 3CRR catalog, we found excellent agreement of R with optical type, infrared flux ratios, and optical polarization. This indicates that probably both R and optical classification are very good orientation indicators, and the unified model is strongly predictive for these objects. The relative number densities indicate half-opening angles close to 60°, as expected from large surveys. The separations of optical types according to radio core dominance as well as near-infrared/MIR ratios, which are essentially perfect, mean that there can be only a small dispersion of torus half-opening angles. Also, even though torus dust is thought to be clumpy, there is an almost zero probability to see a type-1 source at high inclination. Finally, using only the Copernican Principle, i.e., the assumption that solid angle is filled uniformly with source axis orientations, we estimated a semi-empirical relation between core dominance and AGN inclination. This makes it possible to use R to infer the inclination of a source to an accuracy of ∼10° or less, at least for this type of object.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2020

Millimeter-wave Point Sources from the 2500 Square Degree SPT-SZ Survey: Catalog and Population Statistics

In this work, we present a catalog of emissive point sources detected in the SPT-SZ survey, a contiguous 2530- square-degree area surveyed with the South Pole Telescope (SPT) from 2008 - 2011 in three bands centered at 95, 150, and 220GHz. The catalog contains 4845 sources measured at a significance of 4.5σ or greater in at least one band, corresponding to detections above approximately 9.8, 5.8, and 20.4 mJy in 95, 150, and 220 GHz, respectively. Spectral behavior in the SPT bands is used for source classification into two populations based on the underlying physical mechanisms of compact, emissive sources that are bright at millimeter wavelengths: synchrotron radiation from active galactic nuclei and thermal emission from dust. The latter population includes a component of high-redshift sources often referred to as submillimeter galaxies (SMGs). In the relatively bright flux ranges probed by the survey, these sources are expected to be magnified by strong gravitational lensing. The survey also contains sources consistent with protoclusters, groups of dusty galaxies at high redshift undergoing collapse. We cross-match the SPT-SZ catalog with external catalogs at radio, infrared, and X-ray wavelengths and identify available redshift information. The catalog splits into 3980 synchrotron-dominated and 865 dust-dominated sources and we determine a list of 506 SMGs. Ten sources in the catalog are identified as stars. We calculate number counts for the full catalog, and synchrotron and dusty components, using a bootstrap method and compare our measured counts with models. This paper represents the third and final catalog of point sources in the SPT-SZ survey.

79 ASTRONOMY AND ASTROPHYSICS↗
NASA NTRS2000

Behavior of the Aurora During 10-12 May, 1999 When The Solar Wind Nearly Disappeared

The aurora was still active with occasional pseudobreakup events when the solar wind density diminished to unusually small densities (0.2 cc -1) during May 10-12, 1999. The aurora was observed at high magnetic latitudes indicating that the electron precipitation source moved northward as the geomagnetic activity decreased. The events we have studied indicate that the solar wind density alone is not the primary parameter that controls the auroral activity. The weak auroral activity was observed with 150 nT magnetic bays and when the interplanetary magnetic field (IMF) Bz was small and positive resulting in small _ parameter. A new auroral feature was observed on May 11, 1999, between 0900-2000 UT. The electron precipitation was energetic, uniform, and covered the region commonly identified as the polar cap. This precipitation lasted for more than 10 hours and was stable over time scales of tens of minutes. On May 12, as the solar wind began to recover, a prolonged period of dayside activity occurred and was followed by a typical aurora at 0500 UT.

Parks, G.↗
NASA NTRS2010

Automatic Identification of Solar X-Ray Bright Points in Hinode X-Ray Data

We have automated a method that is used to find point sources in Chandra X-ray telescope data, to identify solar bright points in Hinode X-ray data. This tool, called lextrct, first identifies candidate sources that are brighter than the surrounding background. The algorithm also allows selected pixels to be excluded from the source-finding, thus allowing saturated pixels (from flares and/or active regions) to be ignored. We then use lextrct to fit the sources to two-dimensional, elliptical Gaussians. The size and orientation give an approximation of the shape of the bright points. We are in the process of analyzing observations through the Al_poly filter with a four-second exposure time, to obtain a catalogue of bright points, which will include their sizes, lifetimes, intensities, and position on the solar disk

Adams, M. L.↗
DOE OSTI2016

List-Mode source injection algorithm for detectors with arbitrary pose and trajectory

environments, introducing actual sources is usually neither feasible nor economical. It is more practical to move the detector (whether airborne, vehicle-borne, or human-portable) through the test environment and then later artificially superimpose either measured or simulated source signatures on the recorded background data. We present a source injection (SI) algorithm that inserts source events into list-mode background data. It is designed to accommodate cases where “injection data” are available only at a limited set of locations. Stochastic methods are employed both to select source events from a measured dataset containing both source and background events, and to scale the number of selected events to match the field conditions of detector-source distance, air attenuation, acquisition duration, and the relative strength of the measured and injected sources. The algorithm is deployed as part of the Airborne Radiation Enhanced-sensor System (ARES) Advanced Technology Demonstration, and is applied to field data obtained using the ARES detector system. For this helicopter-borne detection system, we describe a practical ground-based measurement scheme for acquiring data for later injection. These data are then injected into background data acquired during flight, where arbitrary detector poses and trajectories are possible. Similar flight paths are traversed with and without actual 137Cs and 60Co sources present. SI is applied to the source-absent datasets, and the results are compared to the source-present datasets. SI output is found to be consistent with measured activity within the estimated uncertainties in GPS position and source strength.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗
DOE OSTI2012

Status of Global Threat Reduction Initiative's Activities Underway to Address Major Domestic Radiological Security Challenges - 12105

During their service lives, radioactive sealed sources are used for a wide variety of essential purposes. However, each year, thousands of radioactive sealed sources that pose a potential risk to national security, health, and safety become disused and unwanted in the United States. Due to their concentrated activity and portability, these sources could be used in radiological dispersal devices ('dirty bombs'). For more than a decade, the National Nuclear Security Administration and the U.S. Department of Energy, through the Global Threat Reduction Initiative Offsite Source Recovery Project (GTRI/OSRP), have facilitated the removal and disposition of thousands of disused/unwanted sources worldwide. However, the ability of GTRI/OSRP to continue its work is critically dependent on the ability to transport and appropriately dispose of these sources. On that front, GTRI/OSRP progress includes development of two prototype Type B transport containers and significant efforts toward certification, increased commercial disposal access for risk-significant sealed sources at commercial sites, and cooperation through the International Atomic Energy Agency to increase source repatriation. Disused sealed sources continue to pose a national security concern. The impact of a dirty bomb detonation could be costly both financially and to those exposed to the resulting radiation. However, significant progress has been made since 2008 on each of the challenges identified in the DHS Sealed Source Security Workshop. Not only will there be increased opportunity for commercial disposal of many sizes and types of sealed sources, but also stakeholders are studying front-end solutions to the problem of disused sealed sources, such as financial assurance and recycle. The lack of sealed source transport containers is also likely to be mitigated with the development and certification by NNSA of two new Type B models. Internationally, increased efforts at source repatriation will mitigate the threat posed by disused sealed sources abroad. Sealed sources provide irreplaceable benefits to those who use them or who benefit from their use; now stakeholders are rising to the challenge of ensuring that those benefits are safely and securely realized. (authors)

07 ISOTOPE AND RADIATION SOURCES↗
DOE OSTI2012

Exploring {sup 57}Co as a new isotope for brachytherapy applications

Purpose: The characteristics of the radionuclide {sup 57}Co make it interesting for use as a brachytherapy source. {sup 57}Co combines a possible high specific activity with the emission of relatively low-energy photons and a half-life (272 days) suitable for regular source exchanges in an afterloader. {sup 57}Co decays by electron capture to the stable {sup 57}Fe with emission of 136 and 122 keV photons. Methods: A hypothetical {sup 57}Co source based on the Flexisource brachytherapy encapsulation with the active core set as a pure cobalt cylinder (length 3.5 mm and diameter 0.6 mm) covered with a cylindrical stainless-steel capsule (length 5 mm and thickness 0.125 mm) was simulated using Geant4 Monte Carlo (MC) code version 9.4. The radial dose function, g(r), and anisotropy function F(r,{theta}), for the line source approximation were calculated following the TG-43U1 formalism. The results were compared to well-known {sup 192}Ir and {sup 125}I radionuclides, representing the higher and the lower energy end of brachytherapy, respectively. Results: The mean energy of photons in water, after passing through the core and the encapsulation material was 123 keV. This hypothetical {sup 57}Co source has an increasing g(r) due to multiple scatter of low-energy photons, which results in a more uniform dose distribution than {sup 192}Ir. Conclusions: {sup 57}Co has many advantages compared to {sup 192}Ir due to its low-energy gamma emissions without any electron contamination. {sup 57}Co has an increasing g(r) that results in a more uniform dose distribution than {sup 192}Ir due to its multiple scattered photons. The anisotropy of the {sup 57}Co source is comparable to that of {sup 192}Ir. Furthermore, {sup 57}Co has lower shielding requirements than {sup 192}Ir.

07 ISOTOPE AND RADIATION SOURCES↗
DOE OSTI2012

PHOTOMETRY AND PHOTOMETRIC REDSHIFT CATALOGS FOR THE LOCKMAN HOLE DEEP FIELD

We present broadband photometry and photometric redshifts for 187,611 sources located in {approx}0.5 deg{sup 2} in the Lockman Hole area. The catalog includes 388 X-ray-detected sources identified with the very deep XMM-Newton observations available for an area of 0.2 deg{sup 2}. The source detection was performed on the R{sub c}-, z'-, and B-band images and the available photometry is spanning from the far-ultraviolet to the mid-infrared, reaching in the best-case scenario 21 bands. Astrometry corrections and photometric cross-calibrations over the entire data set allowed the computation of accurate photometric redshifts. Special treatment is undertaken for the X-ray sources, the majority of which are active galactic nuclei (AGNs). For normal galaxies, comparing the photometric redshifts to the 253 available spectroscopic redshifts, we achieve an accuracy of {sigma}{sub {Delta}z/(1+z)} = 0.036, with 12.6% outliers. For the X-ray-detected sources, compared to 115 spectroscopic redshifts, the accuracy is {sigma}{sub {Delta}z/(1+z)} = 0.069, with 18.3% outliers, where the outliers are defined as sources with |z{sub phot} - z{sub spec}| > 0.15 Multiplication-Sign (1 + z{sub spec}). These results are a significant improvement over the previously available photometric redshifts for normal galaxies in the Lockman Hole, while it is the first time that photometric redshifts are computed and made public for AGNs for this field.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2016

The Chandra COSMOS-Legacy survey: source X-ray spectral properties

We present the X-ray spectral analysis of the 1855 extragalactic sources in the Chandra COSMOS-Legacy survey catalog having more than 30 net counts in the 0.5–7 keV band. A total of 38% of the sources are optically classified type 1 active galactic nuclei (AGNs), 60% are type 2 AGNs, and 2% are passive, low-redshift galaxies. We study the distribution of AGN photon index Γ and of the intrinsic absorption N{sub H,z} based on the sources’ optical classification: type 1 AGNs have a slightly steeper mean photon index Γ than type 2 AGNs, which, on the other hand, have average N{sub H,z} ∼3 times higher than type 1 AGNs. We find that ∼15% of type 1 AGNs have N{sub H,z}>10{sup 22} cm{sup −2}, i.e., are obscured according to the X-ray spectral fitting; the vast majority of these sources have L{sub 2--10keV} > 10{sup 44} erg s{sup −1}. The existence of these objects suggests that optical and X-ray obscuration can be caused by different phenomena, the X-ray obscuration being, for example, caused by dust-free material surrounding the inner part of the nuclei. Approximately 18% of type 2 AGNs have N{sub H,z}<10{sup 22} cm{sup −2}, and most of these sources have low X-ray luminosities (L {sub 2--10keV} < 10{sup 43} erg s{sup −1}). We expect a part of these sources to be low-accretion, unobscured AGNs lacking broad emission lines. Finally, we also find a direct proportional trend between N{sub H,z} and host-galaxy mass and star formation rate, although part of this trend is due to a redshift selection effect.

79 ASTRONOMY AND ASTROPHYSICS↗
NASA NTRS1994

Centaurus A, the core of the problem

The bright, peculiar elliptical galaxy Centaurus A (NGC 5128, PKS 1322-427) was one of the first extragalactic radio sources to be optically identified (Bolton et al. 1949). At a distance of 4 Mpc, Centaurus A is the closest active radio galaxy and affords the highest linear imaging resolution (1 mas approximately equal to 0.02 pc) and hence the best prospects for studying an active nucleus close to the central radio source. We present the results of multi-epoch, 8.4-GHz, very long baseline interferometry (VLBI), imaging observations of the nucleus made over the past three years. The nucleus possesses a core-jet structure where the inner portion of the jet shows apparent linear motion with a velocity substantially less than the speed of light.

Tingay, S. J.↗
DOE OSTI2022

Permafrost Active Layer Microbes From Ny Ålesund, Svalbard (79°N) Show Autotrophic and Heterotrophic Metabolisms With Diverse Carbon-Degrading Enzymes

The active layer of permafrost in Ny Ålesund, Svalbard (79°N) around the Bayelva River in the Leirhaugen glacier moraine is measured as a small net carbon sink at the brink of becoming a carbon source. In many permafrost-dominating ecosystems, microbes in the active layers have been shown to drive organic matter degradation and greenhouse gas production, creating positive feedback on climate change. However, the microbial metabolisms linking the environmental geochemical processes and the populations that perform them have not been fully characterized. In this paper, we present geochemical, enzymatic, and isotopic data paired with 10 Pseudomonas sp. cultures and metagenomic libraries of two active layer soil cores (BPF1 and BPF2) from Ny Ålesund, Svalbard, (79°N). Relative to BPF1, BPF2 had statistically higher C/N ratios (15 ± 1 for BPF1 vs. 29 ± 10 for BPF2; n = 30, p < 10 –5 ), statistically lower organic carbon (2% ± 0.6% for BPF1 vs. 1.6% ± 0.4% for BPF2, p < 0.02), statistically lower nitrogen (0.1% ± 0.03% for BPF1 vs. 0.07% ± 0.02% for BPF2, p < 10 –6 ). The d 13 C values for inorganic carbon did not correlate with those of organic carbon in BPF2, suggesting lower heterotrophic respiration. An increase in the δ 13 C of inorganic carbon with depth either reflects an autotrophic signal or mixing between a heterotrophic source at the surface and a lithotrophic source at depth. Potential enzyme activity of xylosidase and N-acetyl-β-D-glucosaminidase increases twofold at 15°C, relative to 25°C, indicating cold adaptation in the cultures and bulk soil. Potential enzyme activity of leucine aminopeptidase across soils and cultures was two orders of magnitude higher than other tested enzymes, implying that organisms use leucine as a nitrogen and carbon source in this nutrient-limited environment. Besides demonstrating large variability in carbon compositions of permafrost active layer soils only ∼84 m apart, results suggest that the Svalbard active layer microbes are often limited by organic carbon or nitrogen availability and have adaptations to the current environment, and metabolic flexibility to adapt to the warming climate.

54 ENVIRONMENTAL SCIENCES↗