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Microbial Factories for Solar System Exploration

As humans venture deeper into space for longer duration missions, resupply missions become far more difficult and costly. To address this, alternative methods of in situ biomanufacturing can be developed to provide a reliable source of required nutrients. BioNutrients is an ongoing project including three separate flight experiments that aims to provide on-demand nutrient production through 5-year shelf stable microbial factories for astronauts on long-duration missions. The BioNutrients-3 (BN-3) flight experiment aims to demonstrate on-orbit food safety, user-friendly production pack design, and multi-compound production capability. This current study seeks to support the multiple compound production element of BN-3 by investigating the production of multiple compounds via multiple pathways in one organism. An experiment has been designed to modify an existing strain of Saccharomyces boulardii designed by the BioNutrients team to efficiently produce β-carotene to also efficiently produce a second nutrient, riboflavin. This experiment entails the random shuffling and recombination of gene elements including promoters and terminators of various strengths to identify optimal combinations for the upregulation of the riboflavin production pathway. Implementation of this experiment would provide insight into the efficiency of the multiple pathways production method and pave the way for additional fine tuning of the riboflavin production pathway and additional nutrient production pathways in S. boulardii.

Biomanufacturing↗

Elastic wave analyzer for icy sub-surfaces (EWAIS) in the solar system

The sub-surface structures of water-bearing icy worlds (e.g., Europa, Enceladus, and Titan) and the structures of icy regions on Mars contain critical information about their origin and evolution. These bodies have been identified as high-priority targets in the NRC Planetary Science Decadal Survey, which noted a lack of technology readiness as one impediment to related missions. An Elastic Wave Analyzer for Icy Sub-surfaces (EWAIS) instrument is being developed to acquire data that can provide answers to fundamental science questions related to these bodies. The EWAIS instrument generates and receives elastic waves using an array of piezoelectric transducers capable of operating at temperatures as low as 30K. A developed breadboard acquires reflected signals from discontinuities in the traveled materials’ impedance (the product of wave velocity and density) along the wave path. Analysis of the acquired data can be used to determine the elastic properties, presence of cracks, locations of cavities and other discontinuities, as well as the thicknesses of ice and liquid layers. The EWAIS instrument is being developed with a novel dual-frequency transmitter array that will enable reaching distances of kilometers through ice, as well as resolving smaller acoustic reflectors nearer the EWAIS instrument. The selected dual frequencies correspond to two depth ranges that can be analyzed, with resolutions of tens of centimeters in the region of tens to hundreds of meters and tens of meters on the kilometers scale, respectively. The EWAIS will be applicable for operation from any in-situ platform, including surface assets (e.g., lander and rover) and sub-surface assets (e.g., melting probes).

Bar-Cohen, Y.↗