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At least 1,495 records · Page 83

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DOE OSTI2023

Genome editing in the mouse brain with minimally immunogenic Cas9 RNPs

Transient delivery of CRISPR-Cas9 ribonucleoproteins (RNPs) into the central nervous system (CNS) for therapeutic genome editing could avoid limitations of viral vector-based delivery including cargo capacity, immunogenicity, and cost. Here, we tested the ability of cell-penetrant Cas9 RNPs to edit the mouse striatum when introduced using a convection-enhanced delivery system. These transient Cas9 RNPs showed comparable editing of neurons and reduced adaptive immune responses relative to one formulation of Cas9 delivered using AAV serotype 9. The production of ultra-low endotoxin Cas9 protein manufactured at scale further improved innate immunity. We conclude that injection-based delivery of minimally immunogenic CRISPR genome editing RNPs into the CNS provides a valuable alternative to virus-mediated genome editing.

60 APPLIED LIFE SCIENCES↗

DOE OSTI2022

Simulation of the High Temperature Test Facility (HTTF) Core Using the 2D Ring Model with SAM

A two-dimensional ring model is developed with SAM to model the core of the High Temperature Test Facility (HTTF) at the system level. The ring model simplifies the complex structure of the HTTF core by converting the hexagonal rows of heaters and flow channels into layers of concentric annular rings. The ring model is first compared against a 3D-1D model where the solid structures are fully resolved in three dimensions while the fluid structures are modeled as one-dimensional flows. Comparison between the 3D-1D and the ring models shows that the latter can predict major parameters reasonably well under steady-state normal operating condition, but the heater temperatures are underpredicted. Adjustment is made to the effective thermal conductivity of the ceramic core of the ring model to improve the heater temperature predictions. Furthermore, the ring model is also used to simulate a transient Pressurized Conduction Cooldown (PCC) condition and is benchmarked with the experimental data from the HTTF Test PG-27. Good agreement is obtained between the experimental data and the predictions by the ring model.

21 SPECIFIC NUCLEAR REACTORS AND ASSOCIATED PLANTS↗

DOE OSTI2020

Assessment of Dynamic Fracture in Ultra-High Performance Concrete Using Synchrotron X-ray Source

Ultra-high-performance concretes (UHPCs) retain higher ultimate (compressive) strengths and greater durability relative to traditional concrete materials. As a result, they are enabling construction materials for blast resistant structures, where a higher capacity for energy absorption is required. In past investigations, the strength of UHPCs was shown to retain a dependency on strain rate. Still, the failure mechanisms which govern this rate dependency have yet to be completely elucidated. In the current study, the dynamic fracture behavior of Cor-tuf UHPC was assessed, in-situ, using a Synchrotron X-ray source in phase contrast imaging (PCI) mode. Prior to testing, the material’s morphology was characterized using X-ray computed tomography (CT) and scanning electron microscopy (SEM). A polychromatic pulsed X-ray source, high-speed camera and Kolsky bar apparatus were used in tandem, to observe the transient failure modes. A pin was placed at the end of the incident bar to control the location of damage initiation, and tests were performed at a constant velocity of ~6 m/s. These test conditions were observed to yield cracks with median and/or cone like geometries. Despite the variation in the crack paths, a significant difference in peak load was not observed. The failure mode was also observed to depend on the microstructure of the concrete samples.

Kolsky bar↗

DOE OSTI2025

Efficient Simulation of Cascading Outages Using an Energy Function-Embedded Quasi-Steady-State Model

Here, this paper proposed an energy function-embedded quasi-steady-state model for efficient simulation of cascading outages on a power grid while addressing transient stability concerns. Compared to quasi-steady-state models, the proposed model incorporates short-term dynamic simulation and an energy function method to efficiently evaluate the transient stability of a power grid together with outage propagation without transient stability simulation. Cascading outage simulation using the proposed model conducts three steps for each disturbance such as a line outage. First, it performs time-domain simulation for a short term to obtain a post-disturbance trajectory. Second, along the trajectory, the system state with the local maximum potential energy is found and used as the initial point to search for a relevant unstable equilibrium by Newton's method. Third, the transient energy margin is estimated based on this unstable equilibrium to predict an out-of-step condition with generators. The proposed energy function-embedded quasi-steady-state model is tested in terms of its accuracy and time performance on an NPCC 140-bus power system and compared to a quasi-steady-state model embedding transient stability simulation.

Guo, Zhenping [Univ. of Tennessee, Knoxville, TN (↗

NASA NTRSDate not supplied

Hybrid-Electric Aero-Propulsion Controls Testbed Results

NASA is supporting the development of Electrified Aircraft Propulsion (EAP) technology due to its potential to reduce aircraft fuel burn, emissions, and noise as well as improving safety and performance. One focus of this research is the electrification of conventional turbomachinery propulsion systems, which offers ways to improve the performance and operability of turbine-engine powered aircraft through the addition of electro-mechanical systems. These hybrid-electric turbine engines provide additional actuation and energy management control opportunities for improving stability and transient response behavior. This paper summarizes the results of a Hardware-in-the-Loop (HIL) test performed at the NASA Electric Aircraft Testbed (NEAT) during the summer of 2022. The test demonstrates the feasibility and performance of an advanced energy management control strategy by integrating a simulated turbofan engine with scaled electro-mechanical hardware. A full-scale real-time reference model of a geared turbofan was run alongside a scaled electro-mechanical system representing the electrified turbofan components operating at a megawatt-scale power level. The model was interfaced with the hardware through a novel closed-loop control and scaling algorithm that emulated the dynamic speed and torque response of the turbofan shafts. The control strategy was implemented on the electrical machines connected to the emulated turbomachinery shafts. The results from the testbed are compared against simulations that predict the testbed and geared turbofan model operation. The energy management control strategy successfully changed the operating point of the engine model and improved its stability during throttle transients. These results also demonstrate the success of the novel closed loop control and scaling approach for emulating turbomachinery and elevate the Technology Readiness Level (TRL) of the energy management control strategy.

Aeronautics↗

NASA NTRS2023

Hybrid-Electric Aero-Propulsion Controls Testbed Results

Aeronautics↗

NASA NTRS2012

A Turbine Based Combined Cycle Engine Inlet Model and Mode Transition Simulation Based on HiTECC Tool

An inlet system is being tested to evaluate methodologies for a turbine based combined cycle propulsion system to perform a controlled inlet mode transition. Prior to wind tunnel based hardware testing of controlled mode transitions, simulation models are used to test, debug, and validate potential control algorithms. One candidate simulation package for this purpose is the High Mach Transient Engine Cycle Code (HiTECC). The HiTECC simulation package models the inlet system, propulsion systems, thermal energy, geometry, nozzle, and fuel systems. This paper discusses the modification and redesign of the simulation package and control system to represent the NASA large-scale inlet model for Combined Cycle Engine mode transition studies, mounted in NASA Glenn s 10- by 10-Foot Supersonic Wind Tunnel. This model will be used for designing and testing candidate control algorithms before implementation.

Csank, Jeffrey T.↗

NASA NTRS2012

A Turbine Based Combined Cycle Engine Inlet Model and Mode Transition Simulation Based on HiTECC Tool

An inlet system is being tested to evaluate methodologies for a turbine based combined cycle propulsion system to perform a controlled inlet mode transition. Prior to wind tunnel based hardware testing of controlled mode transitions, simulation models are used to test, debug, and validate potential control algorithms. One candidate simulation package for this purpose is the High Mach Transient Engine Cycle Code (HiTECC). The HiTECC simulation package models the inlet system, propulsion systems, thermal energy, geometry, nozzle, and fuel systems. This paper discusses the modification and redesign of the simulation package and control system to represent the NASA large-scale inlet model for Combined Cycle Engine mode transition studies, mounted in NASA Glenn s 10-foot by 10-foot Supersonic Wind Tunnel. This model will be used for designing and testing candidate control algorithms before implementation.

Csank, Jeffrey↗

DOE OSTI2022

Transient Data Library of Solar Grid Integrated Distributed System

This submission contains an open-source library of transient events in distributed system with high solar PV. The library includes the collected data, related documents and scripts for loading the data. The data library is built for transient event detection and machine learning based analysis algorithm development. The data was collected via both field test and software simulation. The units for the data are included in the data file headers for each data series. A text editor or spreadsheet software, such as Excel, and Matlab is required to view the data.

algorithms↗

NASA NTRS2011

Ares I Scale Model Acoustic Test Instrumentation for Acoustic and Pressure Measurements

Ares I Scale Model Acoustic Test (ASMAT) is a 5% scale model test of the Ares I vehicle, launch pad and support structures conducted at MSFC to verify acoustic and ignition environments and evaluate water suppression systems Test design considerations 5% measurements must be scaled to full scale requiring high frequency measurements Users had different frequencies of interest Acoustics: 200 - 2,000 Hz full scale equals 4,000 - 40,000 Hz model scale Ignition Transient: 0 - 100 Hz full scale equals 0 - 2,000 Hz model scale Environment exposure Weather exposure: heat, humidity, thunderstorms, rain, cold and snow Test environments: Plume impingement heat and pressure, and water deluge impingement Several types of sensors were used to measure the environments Different instrument mounts were used according to the location and exposure to the environment This presentation addresses the observed effects of the selected sensors and mount design on the acoustic and pressure measurements

Vargas, Magda B.↗

NASA NTRS2011

Solar Dynamics Observatory Launch and Commissioning

The Solar Dynamics Observatory (SDO) was launched on February 11, 2010. Over the next three months, the spacecraft was raised from its launch orbit into its final geosynchronous orbit and its systems and instruments were tested and calibrated in preparation for its desired ten year science mission studying the Sun. A great deal of activity during this time involved the spacecraft attitude control system (ACS); testing control modes, calibrating sensors and actuators, and using the ACS to help commission the spacecraft instruments and to control the propulsion system as the spacecraft was maneuvered into its final orbit. This paper will discuss the chronology of the SDO launch and commissioning, showing the ACS analysis work performed to diagnose propellant slosh transient and attitude oscillation anomalies that were seen during commissioning, and to determine how to overcome them. The simulations and tests devised to demonstrate correct operation of all onboard ACS modes and the activities in support of instrument calibration will be discussed and the final maneuver plan performed to bring SDO on station will be shown. In addition to detailing these commissioning and anomaly resolution activities, the unique set of tests performed to characterize SDO's on-orbit jitter performance will be discussed.

O'Donnell, James R., Jr.↗

DOE OSTI2017

Refinements of a treat model for transient analysis

In order to prepare for the TREAT reactor being taken off standby, the Gateway for Advanced Modeling and Simulation Lab has used a set of KENO models to simulate certain TREAT temperature- limited transients using the Transient Reactor excursion simulator (T-ReX) code, a hybrid deterministic-stochastic code. These transients are from the M8CAL experiment series, a set of experiments done to calibrate the reactor for the planned M8 series of fuel tests. Recently, the model has been updated in media definitions and geometry. Models were examined and calculations were performed to low uncertainty values to find the k{sub eff} and reactivity insertions. Alterations to modeled values of shutdown and initial transient rod insertion depth were made to create models which had experimental values of reactivity insertion, pre-transient k{sub eff}, or both. It was found that two of the altered media inputs, fuel and Zircaloy-3 cladding, had significant effect on the k{sub eff}. Surprisingly, the Zircaloy-3 composition had a larger effect on k{sub eff}. The reactivity insertions in this model's 2855, 2856 and 2857 temperature-limited transients were found to be above experimental values by a factor of 5.5% to 7%, and that pre-transient k{sub eff} was too high, at 1.002. It was found that increasing shutdown rod insertion by 2 to 2.5 cm decreased pre-transient k{sub eff} to 1.0, and that increasing shutdown rod insertion by 3 to 5 cm and decreasing initial transient rod insertion by 1 to 2 cm gave perfect pre-transient k{sub eff} and total reactivity insertion. (authors)

21 SPECIFIC NUCLEAR REACTORS AND ASSOCIATED PLANTS↗

NASA NTRS2001

Performance Modeling of Experimental Laser Lightcrafts

A computational plasma aerodynamics model is developed to study the performance of a laser propelled Lightcraft. The computational methodology is based on a time-accurate, three-dimensional, finite-difference, chemically reacting, unstructured grid, pressure-based formulation. The underlying physics are added and tested systematically using a building-block approach. The physics modeled include non-equilibrium thermodynamics, non-equilibrium air-plasma finite-rate kinetics, specular ray tracing, laser beam energy absorption and refraction by plasma, non-equilibrium plasma radiation, and plasma resonance. A series of transient computations are performed at several laser pulse energy levels and the simulated physics are discussed and compared with those of tests and literatures. The predicted coupling coefficients for the Lightcraft compared reasonably well with those of tests conducted on a pendulum apparatus.

Wang, Ten-See↗

DOE OSTI2020

Two-temperature warm dense hydrogen as a test of quantum protons driven by orbital-free density functional theory electronic forces

We consider a steady-state (but transient) situation in which a warm dense aggregate is a two-temperature system with equilibrium electrons at temperature Te, ions at Ti, and Te ≠ Ti. Such states are achievable by pump–probe experiments. For warm dense hydrogen in such a two-temperature situation, we investigate nuclear quantum effects (NQEs) on structure and thermodynamic properties, thereby delineating the limitations of ordinary ab initio molecular dynamics. We use path integral molecular dynamics (PIMD) simulations driven by orbital-free density functional theory (OFDFT) calculations with state-of-the-art noninteracting free-energy and exchange-correlation functionals for the explicit temperature dependence. We calibrate the OFDFT calculations against conventional (explicit orbitals) Kohn–Sham DFT. We find that when the ratio of the ionic thermal de Broglie wavelength to the mean interionic distance is larger than about 0.30, the ionic radial distribution function is meaningfully affected by the inclusion of NQEs. Moreover, NQEs induce a substantial increase in both the ionic and electronic pressures. This confirms the importance of NQEs for highly accurate equation-of-state data on highly driven hydrogen. For Te > 20 kK, increasing Te in the warm dense hydrogen has slight effects on the ionic radial distribution function and equation of state in the range of densities considered. In addition, we confirm that compared with thermostatted ring-polymer molecular dynamics, the primitive PIMD algorithm overestimates electronic pressures, a consequence of the overly localized ionic description from the primitive scheme.

71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSIC↗

NASA NTRS1983

Analysis, testing, and evaluation of faulted and unfaulted Wye, Delta, and open Delta connected electromechanical actuators

Mathematical models capable of simulating the transient, steady state, and faulted performance characteristics of various brushless dc machine-PSA (power switching assembly) configurations were developed. These systems are intended for possible future use as primemovers in EMAs (electromechanical actuators) for flight control applications. These machine-PSA configurations include wye, delta, and open-delta connected systems. The research performed under this contract was initially broken down into the following six tasks: development of mathematical models for various machine-PSA configurations; experimental validation of the model for failure modes; experimental validation of the mathematical model for shorted turn-failure modes; tradeoff study; and documentation of results and methodology.

Nehl, T. W.↗

DOE OSTI2018

Investigation of Transient Xenon Processes in VVER-1200 at the Novovoronezh NPP

The results of experimental studies of the integral and spatial transient xenon processes in the No. 6 unit of the Novovoronezh NPP with VVER-1200 are presented. An unconventional method of measurement was tested in the study of integral processes: the course of the xenon process was recorded as the change in the critical concentration of boric acid in the reactor simultaneously with a calculation of the concentration in real-time. The spatial transient processes were studied for the example of axial and diametral free xenon oscillations of energy release in the core.

22 GENERAL STUDIES OF NUCLEAR REACTORS↗

NASA NTRS2000

Performance Modeling of an Experimental Laser Propelled Lightcraft

A computational plasma aerodynamics model is developed to study the performance of an experimental laser propelled lightcraft. The computational methodology is based on a time-accurate, three-dimensional, finite-difference, chemically reacting, unstructured grid, pressure- based formulation. The underlying physics are added and tested systematically using a building-block approach. The physics modeled include non-equilibn'um thermodynamics, non-equilibrium air-plasma finite-rate kinetics, specular ray tracing, laser beam energy absorption and equi refraction by plasma, non-equilibrium plasma radiation, and plasma resonance. A series of transient computations are performed at several laser pulse energy levels and the simulated physics are discussed and compared with those of tests and literature. The predicted coupling coefficients for the lightcraft compared reasonably well with those of tests conducted on a pendulum apparatus.

Wang, Ten-See↗

DOE OSTI2017

Feature selection based on PCA and the transient identification in nuclear power plants - 289

For the sake of enhancing safety and achieving more economic benefits, it is very important to timely and correctly identify the transients during the operation of nuclear power plants. There are thousands of monitoring signals in a nuclear power plant. It is difficult to complete the calculations timely if all the related signals are chosen for an algorithm of transient identification. Moreover, the irrelevant state variables may also do harm to the correctness of the identification process. Thus, one of the key tasks for transient identification is to figure out effective methods to extract the features from state variables. In this paper, we will address an efficient method for feature selection and transient identification based on principal component analysis (PCA). The effectiveness of the method are illustrated by the tests based on the data from the simulator designed for the High Temperature gas-cooled Reactor Pebble-bed Module (HTR-PM), which is under construction in China and the construction will be completed around 2017. (authors)

21 SPECIFIC NUCLEAR REACTORS AND ASSOCIATED PLANTS↗