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NASA NTRS1989

Nondestructive Inspection Of Foam And Multilayer Insulations

Techniques and equipment enable nondestructive inspection of sprayed-on foam and multilayer reflecting thermal insulations on metallic substrates. Technology is applied in factories and laboratories; to inspect insulation on cryogenic tanks and pipes. Equipment includes probe head, several electronic modules that take measurements via electromagnetic and electrostatic sensors in probe head, small computer to store and process signals from modules, and printer.

Krause, Dennis R.↗

NASA NTRS1997

In-Flight Flow Visualization Using Infrared Thermography

The feasibility of remote infrared thermography of aircraft surfaces during flight to visualize the extent of laminar flow on a target aircraft has been examined. In general, it was determined that such thermograms can be taken successfully using an existing airplane/thermography system (NASA Dryden's F-18 with infrared imaging pod) and that the transition pattern and, thus, the extent of laminar flow can be extracted from these thermograms. Depending on the in-flight distance between the F-18 and the target aircraft, the thermograms can have a spatial resolution of as little as 0.1 inches. The field of view provided by the present remote system is superior to that of prior stationary infrared thermography systems mounted in the fuselage or vertical tail of a subject aircraft. An additional advantage of the present experimental technique is that the target aircraft requires no or minimal modifications. An image processing procedure was developed which improves the signal-to-noise ratio of the thermograms. Problems encountered during the analog recording of the thermograms (banding of video images) made it impossible to evaluate the adequacy of the present imaging system and image processing procedure to detect transition on untreated metal surfaces. The high reflectance, high thermal difussivity, and low emittance of metal surfaces tend to degrade the images to an extent that it is very difficult to extract transition information from them. The application of a thin (0.005 inches) self-adhesive insulating film to the surface is shown to solve this problem satisfactorily. In addition to the problem of infrared based transition detection on untreated metal surfaces, future flight tests will also concentrate on the visualization of other flow phenomena such as flow separation and reattachment.

vanDam, C. P.↗

NASA NTRS2002

The developmental segregation of posterior crista and saccular vestibular fibers in mice: a carbocyanine tracer study using confocal microscopy

The developmental segregation of gravistatic input mediated by saccular fibers and of angular acceleration input mediated by posterior crista (PC) fibers was analyzed for the first time in a developing mammal using carbocyanine dye tracing in fixed tissue. The data reveal a more extensive projection of either endorgan in 7-day-old mice (P7) than has previously been reported in adult mammals. While we confirm and extend many previous findings, we also describe a novel segregation of saccular and posterior crista fibers in the anterior half of the medial vestibular nucleus (Mv) not reported before. Our developmental analysis shows a progressive segregation of posterior crista and saccular fibers to their respective discrete projection areas between embryonic day 15 (E15) and birth (P0). Retention of overlap in young adult animals appears to reflect the early embryonic overlap found in most areas. The vestibular projection does not show a topological projection as has been described in many other sensory systems. We propose that the unique projection features of the vestibular endorgans may relate to the transformation of vestibular signals into a motor output in the three neuron reflex arc of the VOR, of which the primary vestibular projection constitutes the first leg.

NASA Discipline Developmental Biology↗

NASA NTRS2001

Measurement of distributed strain and temperature based on higher order and higher mode Bragg conditions

A Bragg grating sensor for measuring distributed strain and temperature at the same time comprises an optical fiber having a single mode operating wavelength region and below a cutoff wavelength of the fiber having a multimode operating wavelength region. A saturated, higher order Bragg grating having first and second order Bragg conditions is fabricated in the optical fiber. The first order of Bragg resonance wavelength of the Bragg grating is within the single mode operating wavelength region of the optical fiber and the second order of Bragg resonance wavelength is below the cutoff wavelength of the fiber within the multimode operating wavelength region. The reflectivities of the saturated Bragg grating at the first and second order Bragg conditions are less than two orders of magnitude of one another. In use, the first and second order Bragg conditions are simultaneously created in the sensor at the respective wavelengths and a signal from the sensor is demodulated with respect to each of the wavelengths corresponding to the first and second order Bragg conditions. Two Bragg conditions have different responsivities to strain and temperature, thus allowing two equations for axial strain and temperature to be found in terms of the measure shifts in the primary and second order Bragg wavelengths. This system of equations can be solved for strain and temperature.

Sirkis, James S.↗

DOE OSTI2018

The structural and dynamical role of water in natural organic matter: A 2H NMR and XRD study

Natural organic matter (NOM) is an important component in many near-surface geochemical environments, and its properties are greatly affected by the incorporation of water. Because of its importance, the macroscopic behavior and effects of water in NOM and soil organic matter (SOM) have been extensively studied using a wide range of experimental and computational methods. The molecular scale structural and dynamical behavior of water in these materials, however, is less well understood. This paper presents a variable temperature 2 H NMR and XRD study of water in Suwannee River NOM and its fulvic acid (FA) and humic acid (HA) fractions that provides new insight into the dynamical behavior of structurally different types of water and exchangeable hydrogen environments in NOM. The results provide a basis for future studies of more complex natural organic materials and the interaction of organic materials with mineral surfaces. Room temperature 2 H NMR spectra of samples hydrated in 2 H 2 O and then dehydrated, distinguish 2 H 2 O molecules that are in rapid reorientational motion (correlation times, ν c, > 10 5 Hz), 2 H exchanged onto carboxylic sites of the NOM that do not undergo rapid reorientation at frequencies >~10 3 Hz, and 2 H exchanged onto phenolic and possibly other alcohol sites of the NOM that undergoes rapid, but anisotropic, dynamical reorientation. For samples exposed to water and not dried, the XRD results collected at temperatures from 173 to 298 K show the formation of ice-1h in samples exposed to 100% relative humidity (R.H.) but not in samples exposed to 43% R.H. 2 H NMR of those samples collected at temperatures from 313 K to 173 K show the presence of multiple sites. Near room temperature, the spectra contain a narrow resonance for mobile water undergoing rapid isotropic motion, and a broader symmetrical resonance probably due to a combination of more dynamically restricted water molecules and 2 H exchanged onto phenolic and alcohol functional groups undergoing rapid anisotropic motion. The 43% R.H. samples also yield a broader, quadrupole-dominated, resonance for 2 H exchanged onto functional groups of the NOM. With decreasing temperature the resonances for dynamically restricted water molecules and 2 H exchanged onto phenolic and alcohol functional groups become broader, reflecting a decreasing rate of exchange between the water molecules and functional groups and a decreasing rate of reorientation of the 2 H 2 O molecules. The formation of ice-1h is directly reflected in the 2 H spectra of the 100% R.H. samples as a resonance with a quadrupole coupling constant (QCC) of ~180 kHz. For the 43% R.H. samples, there is also a broad, poorly resolved resonance with typical QCCs of ~180 kHz for which the relative signal intensity increases with decreasing temperature. This signal represents 2 H 2 O molecules that are not crystallized in ice-1h but have greatly reduced reorientation frequencies at low temperature and a hydrogen bonding network with hydrogen bond strengths similar to, but somewhat weaker than, ice-1h. Such molecules are also likely to be present in the 100% R.H. samples. At both R.H.s, some of the 2 H 2 O molecules do not freeze and retain their isotropic motion down to 173 K, the lowest temperature investigated.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

DOE OSTI2016

Induction of oxidative and nitrosative stresses in human retinal pigment epithelial cells by all-trans-retinal

Delayed clearance of free form all-trans-retinal (atRAL) is estimated be the key cause of retinal pigment epithelium (RPE) cells injury during the pathogenesis of retinopathies such as age-related macular degeneration (AMD), however, the underlying molecular mechanisms are far from clear. In this study, we investigated the cytotoxicity effect and underlying molecular mechanism of atRAL on human retinal pigment epithelium ARPE-19 cells. The results indicated that atRAL could cause cell dysfunction by inducing oxidative and nitrosative stresses in ARPE-19 cells. The oxidative stress induced by atRAL was mediated through up-regulation of reactive oxygen species (ROS) generation, activating mitochondrial-dependent and MAPKs signaling pathways, and finally resulting in apoptosis of ARPE-19 cells. The NADPH oxidase inhibitor apocynin could partly attenuated ROS generation, indicating that NADPH oxidase activity was involved in atRAL-induced oxidative stress in ARPE-19 cells. The nitrosative stress induced by atRAL was mainly reflected in increasing nitric oxide (NO) production, enhancing iNOS, ICAM-1 and VCAM-1 expressions, and promoting monocyte adhesion. Furthermore, above effects could be dramatically blocked by using a nuclear factor kappa B (NF-κB) inhibitor SN50, indicated that atRAL-induced oxidative and nitrosative stresses were mediated by NF-κB. The results provide better understanding of atRAL-induced toxicity in human RPE cells. - Highlights: • atRAL induces oxidative stress-mediated apoptosis in ARPE-19 cells. • atRAL induces oxidative stress-mediated inflammation in ARPE-19 cells. • NF-κB is involved in atRAL-induced oxidative and nitrosative stresses.

60 APPLIED LIFE SCIENCES↗

DOE OSTI2019

Transcriptomic atlas of mushroom development reveals conserved genes behind complex multicellularity in fungi

The evolution of complex multicellularity has been one of the major transitions in the history of life. In contrast to simple multicellular aggregates of cells, it has evolved only in a handful of lineages, including animals, embryophytes, red and brown algae, and fungi. Despite being a key step toward the evolution of complex organisms, the evolutionary origins and the genetic underpinnings of complex multicellularity are incompletely known. The development of fungal fruiting bodies from a hyphal thallus represents a transition from simple to complex multicellularity that is inducible under laboratory conditions. We constructed a reference atlas of mushroom formation based on developmental transcriptome data of six species and comparisons of >200 whole genomes, to elucidate the core genetic program of complex multicellularity and fruiting body development in mushroom-forming fungi (Agaricomycetes). Nearly 300 conserved gene families and >70 functional groups contained developmentally regulated genes from five to six species, covering functions related to fungal cell wall remodeling, targeted protein degradation, signal transduction, adhesion, and small secreted proteins (including effector-like orphan genes). Several of these families, including F-box proteins, expansin-like proteins, protein kinases, and transcription factors, showed expansions in Agaricomycetes, many of which convergently expanded in multicellular plants and/or animals too, reflecting convergent solutions to genetic hurdles imposed by complex multicellularity among independently evolved lineages. Finally, this study provides an entry point to studying mushroom development and complex multicellularity in one of the largest clades of complex eukaryotic organisms.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2023

A Deep Learning Filter for the Intraseasonal Variability of the Tropics

Abstract This paper presents a novel application of convolutional neural network (CNN) models for filtering the intraseasonal variability of the tropical atmosphere. In this deep learning filter, two convolutional layers are applied sequentially in a supervised machine learning framework to extract the intraseasonal signal from the total daily anomalies. The CNN-based filter can be tailored for each field similarly to fast Fourier transform filtering methods. When applied to two different fields (zonal wind stress and outgoing longwave radiation), the index of agreement between the filtered signal obtained using the CNN-based filter and a conventional weight-based filter is between 95% and 99%. The advantage of the CNN-based filter over the conventional filters is its applicability to time series with the length comparable to the period of the signal being extracted. Significance Statement This study proposes a new method for discovering hidden connections in data representative of tropical atmosphere variability. The method makes use of an artificial intelligence (AI) algorithm that combines a mathematical operation known as convolution with a mathematical model built to reflect the behavior of the human brain known as artificial neural network. Our results show that the filtered data produced by the AI-based method are consistent with the results obtained using conventional mathematical algorithms. The advantage of the AI-based method is that it can be applied to cases for which the conventional methods have limitations, such as forecast (hindcast) data or real-time monitoring of tropical variability in the 20–100-day range.

Stan, Cristiana↗

NASA NTRS1975

Pulse width fluctuations in mode-locked Nd:YAG lasers

The experiment consisted of a flashlamp-pumped Nd:YAG laser rod in the Brewster-Brewster configuration, passively modelocked by a thick flowing dye in contact with the high reflectivity rear mirror. A single pulse was selected by a cylindrical-ring electrode Pockel's cell, and amplified by two Nd:YAG amplifiers. Calibrated photodiodes measured the total pulse energy at the fundamental frequency and the total pulse energy at the second harmonic frequency. The signals were monitored by a digital data acquisition system and displayed on a teletype printer.

Bechtel, J. H.↗

NASA NTRS1978

Stratigraphic correlation of the radar-detected subsurface interface in Mare Crisium

The delineation of spectrally distinct units in Mare Crisium allows a more complete interpretation of the single subsurface interface detected by the Apollo lunar sounder experiment. This interface is approximately 1400 m below the surface in the central mare region of Crisium, and 1000 m below the surface in the eastern bench region. Based on the power of the returned signal, and the small difference in dielectric constant of returned lunar basalts, it is probable that this interface represents a buried regolith between two major basin-filling episodes. The contact between spectral Units 1 and 2 is the most likely cause for the radar-reflecting interface. Based on this model, the thickness of Unit 3 is 400 m in the eartern part of the mare, and that of Unit 2 is 1000 m. Estimates of the original depth/diameter relations of Pierce are consistent with excavation of an older basalt from below a depth of 1400 m.

Maxwell, T. A.↗

NASA NTRS1990

Compact Sunshade For Telescope Antenna

Proposed built-in sunshade enables large-aperture reflecting telescope to view laser transmitter apparently close to Sun, without adding excessive size or mass to telescope. Telescope looks through sunshade from behind and below. Tops of hexagonal tubes trimmed to spherical shape corresponding to sphere of rotation of telescope. Sunshade supports secondary reflector. Discerns signals from sources only 12 degrees from line of sight to Sun. Sunshade equipped with internal vanes running lengths of tubes receives signals from sources within 6 degree or even 3 degree of apparent position of Sun.

Kerr, E. L.↗

NASA NTRS2003

Interannual Variability of the Tropical Water Cycle: Capabilities in the TRMM Era and Challenges for GPM

Considerable uncertainty surrounds the issue of whether precipitation over the tropical oceans (30" NE) systematically changes with interannual sea-surface temperature (SST) anomalies that accompany El Nino (warm) and La Nina (cold) events. Although it is well documented that El Nino-Southern Oscillation (ENSO) events with marked SST changes over the tropical oceans, produce significant regional changes in precipitation, water vapor, and radiative fluxes in the tropics, we still cannot yet adequately quantify the associated net integrated changes to water and heat balance over the entire tropical oceanic or land sectors. Robertson et al., [2001 GRL] for example, showed that substantial disagreement exists among contemporary satellite estimates of interannual variations in tropical rainfall that are associated with SST changes. Berg et al., [2002 J. Climate] have documented the distinct differences between precipitation structure over the eastern and western Pacific ITCZ and noted how various satellite precipitation algorithms may respond quite differently to ENSO modulations of these precipitation regimes. Resolving this uncertainty is important since precipitation and latent heat release variations over land and ocean sectors are key components of the tropical heat balance in its most aggregated form. Rainfall estimates from the Tropical Rainfall Measuring Mission (TRMM) Precipitation Radar (PR) averaged over the tropical oceans have not solved this issue and, in fact, show marked differences with estimates from two TRMM Microwave Imager (TMI) passive microwave algorithms. In this paper we will focus on findings that uncertainties in microphysical assumptions necessitated by the single-frequency PR measurement pose difficulties for detecting climate-related precipitation signals. Recent work has shown that path-integrated attenuation derived from the effects of precipitation on the radar return from the ocean surface exhibits interannual variability that agrees closely with the TMI time series, yet the PR rainfall interannual variability (and attenuation derived predominantly from reflectivity) differs even in sign. We will explore these apparent inconsistencies and detail their impact on estimates of how ENSO events perturb the tropical rainfall. We will place these results in perspective by considering requirements for precipitation accuracy for global climate variability and change studies involving ENSO, monsoon dynamics and variations, and climate model improvement and validation. The discussion will conclude with an assessment of the implications of these findings for Global Precipitation Mission (GPM) requirements.

Robertson, Franklin R.↗

NASA NTRS2004

THOR: Cloud Thickness from Off beam Lidar Returns

Conventional wisdom is that lidar pulses do not significantly penetrate clouds having optical thickness exceeding about tau = 2, and that no returns are detectable from more than a shallow skin depth. Yet optically thicker clouds of tau much greater than 2 reflect a larger fraction of visible photons, and account for much of Earth s global average albedo. As cloud layer thickness grows, an increasing fraction of reflected photons are scattered multiple times within the cloud, and return from a diffuse concentric halo that grows around the incident pulse, increasing in horizontal area with layer physical thickness. The reflected halo is largely undetected by narrow field-of-view (FoV) receivers commonly used in lidar applications. THOR - Thickness from Off-beam Returns - is an airborne wide-angle detection system with multiple FoVs, capable of observing the diffuse halo, detecting wide-angle signal from which physical thickness of optically thick clouds can be retrieved. In this paper we describe the THOR system, demonstrate that the halo signal is stronger for thicker clouds, and validate physical thickness retrievals for clouds having z > 20, from NASA P-3B flights over the Department of Energy/Atmospheric Radiation Measurement/Southern Great Plains site, using the lidar, radar and other ancillary ground-based data.

Cahalan, Robert F.↗

NASA NTRS2005

Interannual Variability of Tropical Rainfall as Seen From TRMM

Considerable uncertainty surrounds the issue of whether precipitation over the tropical oceans (30deg N/S) systematically changes with interannual sea-surface temperature (SST) anomalies that accompany El Nino (warm) and La Nina (cold) events. Although it is well documented that El Nino-Southern Oscillation (ENSO) events with marked SST changes over the tropical oceans produce significant regional changes in precipitation, water vapor, and radiative fluxes in the tropics, we still cannot yet adequately quantify the associated net integrated changes to water and heat balance over the entire tropical oceanic or land sectors. Resolving this uncertainty is important since precipitation and latent heat release variations over land and ocean sectors are key components of the tropical heat balance in its most aggregated form. Rainfall estimates from the Version 5 Tropical Rainfall Measuring Mission (TRMM) Precipitation Radar (PR) averaged over the tropical oceans have not solved this issue and, in fact, show marked differences with estimates from two TRMM Microwave Imager (TMI) passive microwave algorithms. In this paper we will focus on findings that uncertainties in microphysical assumptions necessitated by the single-frequency PR measurement pose difficulties for detecting climate-related precipitation signals. Recent work has shown that path-integrated attenuation derived from the effects of precipitation on the radar return from the ocean surface exhibits interannual variability that agrees closely with the TMI time series, yet the PR rainfall interannual variability (and attenuation derived predominantly from reflectivity) differs even in sign. We will explore these apparent inconsistencies and examine changes in new TRMM Version 6 retrievals. To place these results in a tropical water balance perspective we also examine interannual variations in evaporation over the tropical oceans made from TRMM and SSM/I (Special Sensor Microwave Imager) measurements of surface winds and humidity. Evaporation estimates from reanalysis and several global model experiments will also be compared to the TRMM findings and evaluated for consistency. The ability to detect regional shifts in freshwater flux over the oceans (equivalently, integrated moisture convergence) and moisture transport will be discussed.

Robertson, Franklin R.↗

NASA NTRS2015

Lunar Plant Experiment: Testing Plant Germination in Lunar Gravity and Ionizing Radiation

We present an open design for a first plant growth module on the Moon (LPX). The primary science goal of lunar habitat is to investigate germination and initial plant growth when subject to the combined effects of lunar gravity and lunar surface radiation. The LPX module has been designed to be a flexible base unit that can be adapted to fly to the lunar surface on a variety of landers and rovers. LPX has size and shape of a 1 Unit CubeSat (10 centimeters on a side) and a total mass of 0.3 kilograms. The base will contain a seed module holding around 50 Arabidopsis seeds and provide approximately 0.5 liters of normal air at standard pressure. By the action of a small pump, water will be released after landing to initiate germination. Images will document the germination, initial growth, phototropism, and circumnutation of the small plants while sunlight remains available. A CO2 sensor tracks the release of CO2 during germination and the subsequent uptake of CO2 by photosynthesis. Simulations indicate that the CO2 peak level will be an increase by 2250 parts per million after 2 earth-days and then decline to about 1000 parts per million after 7 earth-days. The camera and CO2 sensor interfaces are USB. The water pump initiation requires a 5-volt signal. After initiation of germination by the addition of water, the habitat must be maintained above 22 degrees Centigrade to allow for plant growth and below 27 degrees Centigrade to prevent damage to the plants. This relatively tight temperature tolerance will require thermal control systems using reflecting surfaces and insulation that must be specifically designed for each lander or rover. The plant growth unit will function to tilt angles of 20 degrees along any axis. Total image data volume required to gauge the plant growth rate and leaf area is 1 megabyte (minimum data required) to 40 megabytes (preferred data). The lid of the habitat will allow adequate sunlight for plant growth. To provide for optimum growth, light levels in the plant growth stage need to be between 75 and 150 micromoles per meter squared per second interval.

first plant growth↗

NASA NTRS2019

Real Time HABs Mapping Using NASA Glenn Hyperspectral Imager

The hyperspectral imaging system (HSI) developed by the NASA Glenn Research Center was used from 2015- 2017 to collect high spatial resolution data over Lake Erie and the Ohio River. Paired with a vicarious correction approach implemented by the Michigan Tech Research Institute, radiance data collected by the HSI system can be converted to high quality reflectance data which can be used to generate near-real time (within 24 hours) products for the monitoring of harmful algal blooms using existing algorithms. The vicarious correction method relies on imaging a spectrally constant target to normalize HSI data for atmospheric and instrument calibration signals. A large asphalt parking lot near the Western Basin of Lake Erie was spectrally characterized and was determined to be a suitable correction target. Due to the HSI deployment aboard an aircraft, it is able to provide unique insights into water quality conditions not offered by space-based solutions. Aircraft can operate under cloud cover and flight paths can be chosen and changed on-demand, allowing for far more flexibility than space-based platforms. The HSI is also able to collect data at a high spatial resolution (~1 m), allowing for the monitoring of small water bodies, the ability to detect small patches of surface scum, and the capability to monitor the proximity of blooms to targets of interest such as water intakes. With this new rapid turnaround time, airborne data can serve as a complementary monitoring tool to existing satellite platforms, targeting critical areas and responding to bloom events on-demand.

Reid W Sawtell↗

NASA NTRS2019

Real Time HABs Mapping Using NASA Glenn Hyperspectral Imager

Reid W Sawtell↗

DOE OSTI2018

Final Report: Advanced Detector Technologies for Precision Measurement of Inflation, Dark Energy, and Neutrino Masses with the Cosmic Microwave Background

Precision measurements of the Cosmic Microwave Background (CMB) provide unparalleled op- portunities to probe the conditions in the early universe and thereby explore particle physics and gravity at the highest energies yet; measure the number of neutrino species and the sum of the neutrino masses with sensitivity beyond what is achievable with planned terrestrial experiments; improve our knowledge of dark energy; and search for other forms of new physics. The P-5 panel recognized the importance and potential of future CMB observations and recommended the DOE fund a stage four CMB experiment (CMB-S4) under all budget scenarios with construction commencing near the end of this decade. The objective of this proposal is to develop and demonstrate new technologies in time to maximize the science return of CMB-S4. The proposed technologies include: (i) broad band metamaterial antireflection coatings on relevant lenses with negligible absorptive and reflective losses; (ii) filters that reduce out of band optical loading with near perfect in band transmission; (iii) broad-band continuously spinning metamaterial half wave plates that boost the sensitivity to inflationary gravitational waves on large angular scales and mitigate systematics with minimal penalty to the instantaneous sensitivity; and (iv) broad band detectors that will enable excellent foreground discrimination while simultaneously maximizing the sensitivity to the signals from inflation and neutrinos. Together these technologies can realize significant improvements in instrumental sensitivity and control of systematic effects. This improvement in raw sensitivity could be used to reduce costs without sacrificing science by deploying a smaller number of more sensitive detectors, or to improve the sensitivity of CMB-S4 to physics parameters including reducing the error bar on r by the inverse of the mapping speed improvement. Beyond raw sensitivity these technologies address key systematics including polarization mixing and galactic foregrounds and enable CMB-S4 to test the shape of the polarization power spectrum out to larger angular scales further boosting measurements of inflation. In this report we detail the progress that has been made in these technical areas. This includes: (i) a demonstration of 4:1 bandwidth AR coating technology and a detailed understanding of the production challenges, (ii) prototype filters that incorporate absorptive and reflective components into silicon, (iii) a prototype broad band HWP fabricated from metamaterial silicon, and (iv) detector designs spanning 20-350 GHz with an understanding of the difficulties associated with bandwidths greater than one octave.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗