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NASA NTRS2009

Evolved Gas Measurements Planned for the Lower Layers of the Gale Crater Mound with the Sample Analysis at Mars Instrument Suite

The lower mound strata of Gale Crater provide a diverse set of chemical environments for exploration by the varied tools of the Curiosity Rover of the Mars Science Laboratory (MSL) Mission. Orbital imaging and spectroscopy clearly reveal distinct layers of hydrated minerals, sulfates, and clays with abundant evidence of a variety of fluvial processes. The three instruments of the MSL Sample Analysis at aMars (SAM) investigation, the Quadrupole Mass Spectrometer (QMS), the Tunable Laser Spectrometer (TLS), and the Gas Chromatograph (GC) are designed to analyze either atmospheric gases or volatiles thermally evolved or chemically extracted from powdered rock or soil. The presence or absence of organic compounds in these layers is of great interest since such an in situ search for this type of record has not been successfully implemented since the mid-60s Viking GCMS experiments. However, regardless of the outcome of the analysis for organics, the abundance and isotopic composition of thermally evolved inorganic compounds should also provide a rich data set to complement the mineralogical and elemental information provided by other MSL instruments. In addition, these evolved gas analysis (EGA) experiments will help test sedimentary models proposed by Malin and Edgett (2000) and then further developed by Milliken et al (2010) for Gale Crater. In the SAM EGA experiments the evolution temperatures of H2O, CO2, SO2, O2, or other simple compounds as the samples are heated in a helium stream to 1000 C provides information on mineral types and their associations. The isotopic composition of O, H, C, and S can be precisely determined in several evolved compounds and compared with the present day atmosphere. Such SAM results might be able to test mineralogical evidence of changing sedimentary and alteration processes over an extended period of time. For example, Bibring et al (2006) have suggested such a major shift from early nonacidic to later acidic alteration. We will illustrate through a variety of evolved gas experiments implemented under SAM-like gas flow and temperature ramp conditions on terrestrial analog minerals on high fidelity Sam breadboards the type of chemical information we expect SAM to provide.

Mahaffy, Paul↗

DOE OSTI2017

A Universal Correlation between Star Formation Activity and Molecular Gas Properties Across Environments

We present the molecular gas mass fraction (f{sub H{sub 2}}) and star formation efficiency (SFE) of local galaxies on the basis of our new CO(J=1−0) observations with the Nobeyama 45 m radio telescope, combined with the COLDGASS galaxy catalog, as a function of galaxy environment defined as the local number density of galaxies measured with SDSS DR7 spectroscopic data. Our sample covers a wide range in the stellar mass and star formation rate (SFR), and also covers a wide environmental range over two orders of magnitude. This allows us to conduct the first systematic study of environmental dependence of molecular gas properties in galaxies from the lowest- to the highest-density environments in the local universe. We confirm that both f{sub H{sub 2}} and SFE have strong positive correlations with the SFR offset from the star-forming main sequence (ΔMS) and, most importantly, we find that these correlations are universal across all environments. Our result demonstrates that star formation activity within individual galaxies is primarily controlled by their molecular gas content, regardless of their global environment. Therefore, we claim that one always needs to be careful about the ΔMS distribution of the sample when investigating the environmental effects on the H{sub 2} gas content in galaxies.

79 ASTRONOMY AND ASTROPHYSICS↗

DOE OSTI2016

Transmission diamond imaging detector

Many modern synchrotron techniques are trending toward use of high flux beams and/or beams which require enhanced stability and precise understanding of beam position and intensity from the front end of the beamline all the way to the sample. For high flux beams, major challenges include heat load management in optics (including the vacuum windows) and a mechanism of real-time volumetric measurement of beam properties such as flux, position, and morphology. For beam stability in these environments, feedback from such measurements directly to control systems for optical elements or to sample positioning stages would be invaluable. To address these challenges, we are developing diamond-based instrumented vacuum windows with integrated volumetric x-ray intensity, beam profile and beam-position monitoring capabilities. A 50 µm thick single crystal diamond has been lithographically patterned to produce 60 µm pixels, creating a >1kilopixel free-standing transmission imaging detector. This device, coupled with a custom, FPGA-based readout, has been used to image both white and monochromatic x-ray beams and capture the last x-ray photons at the National Synchrotron Light Source (NSLS). This technology will form the basis for the instrumented end-station window of the x-ray footprinting beamline (XFP) at NSLS-II.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

DOE OSTI2018

SPRUCE Small Differences in Ombrotrophy Control Regional-Scale Variation in Methane Cycling among Sphagnum-Dominated Peatlands: Supporting Data

This dataset contains net ecosystem respiration and the isotopic composition of emitted methane (CH4) form flux chambers as well as anions and cations from ion chromatography. Samples were collected from the Spruce and Peatland Responses Under Changing Environments (SPRUCE) experimental site within the Marcell Experimental Forest in northern Minnesota, USA from June 2013 to June 2014 (2013-06-01 to 2014-06-30). This dataset were used in analyses in Zalman et al. (2018), which reported on the mechanisms controlling carbon (C) cycling rates and greenhouse gas emissions using a combination of biogeochemical and microbial approaches in three low pH, Sphagnum-dominated peatlands in northern Minnesota. In particular, biogeochemical parameters (CH4 and carbon dioxide (CO2) production potentials, porewater and gas CH4 and CO2 concentrations, and associated isotopic signatures), soil chemistry (organic acids and phenolics), and microbial community characteristics (quantification of methanogen and methanotroph abundance and activity) over a 1-year period in these three peatlands. The objective of this intensive study was to analyze biogeochemical and microbial characteristics of three outwardly similar low pH, Sphagnum-dominated peatlands to determine key characteristics that could explain differences in anaerobic C cycling and net CH4 flux despite their close geographical proximity and similar soil pH and plant communities. This dataset contains two data files in comma-separate values (*.csv) format. Additional metadata are provided: two data dictionaries and a file-level metadata file in comma-separate values (.csv) format and a user guide in PDF (*.pdf) format. All figures from Zalman et al. (2018) and all additional online resources are provided for completeness in the user guide. During the August 2026 update to this dataset, the ‘Sampling_Date’ column in the data files was changed from MM/DD/YYYY to YYYY-MM-DD format. No data values were altered

54 ENVIRONMENTAL SCIENCES↗

DOE OSTI2016

SPRUCE Enclosure Corral and Sump System: Description, Operation, and Calibration

This document describes the subsurface corral system that has been designed and constructed to measure water flow and allow the collection of water samples from the outflow of each experimental enclosure at the Spruce and Peatland Responses Under Changing Environments (SPRUCE) experimental site within the Marcell Experimental Forest in northern Minnesota, USA. SPRUCE implemented 10 years of whole-ecosystem warming, deep peat heating, and elevated carbon dioxide (CO2) treatments from 2015-2025 to assess the response of northern peatland ecosystems to increases in temperature and exposures to elevated atmospheric CO2 concentrations. This document: 1. Describes belowground corrals, sump systems, and the operation of sump systems, which are used to calculate the volumes of water that naturally drain enclosures and to trigger automatic collection of water samples; 2. Describes the calibration between water level and water volume in the sump system. This relationship is used to calculate near-surface lateral runoff (“outflow” in L/s) from each enclosure by measuring the change in water level over time. The water volume vs. water level relationship must be re-calibrated if water level sensors (or components) are replaced; and 3. Describes the data and data products that are and will be available from outflow measurements.

54 ENVIRONMENTAL SCIENCES↗

NASA NTRS2005

Ultrasonic Micro-Blades for the Rapid Extraction of Impact Tracks from Aerogel

The science return of NASA's Stardust Mission with its valuable cargo of cometary debris hinges on the ability to efficiently extract particles from silica aerogel collectors. The current method for extracting cosmic dust impact tracks is a mature procedure involving sequential perforation of the aerogel with glass needles on computer controlled micromanipulators. This method is highly successful at removing well-defined aerogel fragments of reasonable optical clarity while causing minimal damage to the surrounding aerogel collector tile. Such a system will be adopted by the JSC Astromaterials Curation Facility in anticipation of Stardust s arrival in early 2006. In addition to Stardust, aerogel is a possible collector for future sample return missions and is used for capture of hypervelocity ejecta in high power laser experiments of interest to LLNL. Researchers will be eager to obtain Stardust samples for study as quickly as possible, and rapid extraction tools requiring little construction, training, or investment would be an attractive asset. To this end, we have experimented with micro-blades for the Stardust impact track extraction process. Our ultimate goal is a rapid extraction system in a clean electron beam environment, such as an SEM or dual-beam FIB, for in situ sample preparation, mounting and analysis.

Ishii, H. A.↗

NASA NTRS2020

The fate of organic carbon in marine sediments - New insights from recent data and analysis

Organic carbon in marine sediments is a critical component of the global carbon cycle, and its degradation influences a wide range of phenomena, including the magnitude of carbon sequestration over geologic timescales, the recycling of inorganic carbon and nutrients, the dissolution and precipitation of carbonates, the production of methane and the nature of the seafloor biosphere. Although much has been learned about the factors that promote and hinder rates of organic carbon degradation in natural systems, the controls on the distribution of organic carbon in modern and ancient sediments are still not fully understood. In this review, we summarize how recent findings are changing entrenched perspectives on organic matter degradation in marine sediments: a shift from a structurally-based chemical reactivity viewpoint towards an emerging acceptance of the role of the ecosystem in organic matter degradation rates. That is, organic carbon has a range of reactivities determined by not only the nature of the organic compounds, but by the biological, geochemical, and physical attributes of its environment. This shift in mindset has gradually come about due to a greater diversity of sample sites, the molecular revolution in biology, discoveries concerning the extent and limits of life, advances in quantitative modeling, investigations of ocean carbon cycling under a variety of extreme paleo-conditions (e.g. greenhouse environments, euxinic/anoxic oceans), the application of novel analytical techniques and interdisciplinary efforts. Adopting this view across scientific disciplines will enable additional progress in understanding how marine sediments influence the global carbon cycle.

Organic carbon↗

NASA NTRSDate not supplied

Small Particle Glovebox Experiments - Preliminary Results

A substantial portion of the asteroid samples returned by the HAYABUSA2 and OSIRIS-REx missions will consist of small (submm) components (due to the collection of those particles on the asteroid surface and due to the fragmenting of larger, friable material post-collection). In order to minimize the alteration/oxidation of asteroid regolith, the bulk collections will be stored and curated in nitrogen purged gloveboxes. The processing of small particles in an N2 glovebox will present challenges that are different from those experienced during lunar and meteorite sample processing. Particles in this size range are susceptible to unpredictable electrostatic charging that can result in sample loss during processing operations. Methods for the handling of sub-mm particles have been well developed for environments with ambient atmospheric conditions and relative humidity (RH) ranges between 40-70%. In such conditions, a number of factors can be successfully employed to minimize the effects of triboelectric charging, including the use of Po-210 sources that neutralize excess charge and the utilization of conductive manipulation tools and sample substrates. However, relative humidity levels above 40% also contribute significantly to dissipation of triboelectric effects. We had not previously investigated our charge mitigation methods in a completely dry, nitrogen-purged environment, or whether they would be sufficient in enabling the successful processing of sub-mm samples. Current glovebox configurations in use in our lunar and meteorite curation laboratories are optimized for the processing of macroscopic samples and tools; these glovebox designs are likely unsuitable for the processing of collections for which the bulk collection is comprised of sub-mm components. Small particle sample preparation requires the use of an optical magnification instrument – typically a stereo binocular microscope with at least 20x magnification. Current glovebox designs are not optimized for the utilization or integration of stereo microscopes; while many current cabinets include microscope viewports that enable the use of small, externally mounted stereo microscopes, the focusing methods (usually involving the use of a lab jack) lack the fidelity and precision required for small particle manipulation and imaging. Working distances of higher (>50x magnification) objective lenses may preclude the external use of a stereo microscope through a viewport; in order to successfully manipulate and image very small (< 20m) particles, stereo and digital microscope systems that are integrated within the glovebox should be investigated. Finally, ergonomic considerations for small particle work within a glovebox must be considered to minimize risk of injury to sample processors. In order to investigate some of the unknown parameters relating to small particle processing within an N2 glovebox, we conducted preliminary, qualitative experiments utilizing a small lunar cabinet that was originally used for film development.

C. J. Snead↗

NASA NTRS2015

Solar Flare Track Exposure Ages in Regolith Particles: A Calibration for Transmission Electron Microscope Measurements

Mineral grains in lunar and asteroidal regolith samples provide a unique record of their interaction with the space environment. Space weathering effects result from multiple processes including: exposure to the solar wind, which results in ion damage and implantation effects that are preserved in the rims of grains (typically the outermost 100 nm); cosmic ray and solar flare activity, which result in track formation; and impact processes that result in the accumulation of vapor-deposited elements, impact melts and adhering grains on particle surfaces. Determining the rate at which these effects accumulate in the grains during their space exposure is critical to studies of the surface evolution of airless bodies. Solar flare energetic particles (mainly Fe-group nuclei) have a penetration depth of a few millimeters and leave a trail of ionization damage in insulating materials that is readily observable by transmission electron microscope (TEM) imaging. The density of solar flare particle tracks is used to infer the length of time an object was at or near the regolith surface (i.e., its exposure age). Track measurements by TEM methods are routine, yet track production rate calibrations have only been determined using chemical etching techniques [e.g., 1, and references therein]. We used focused ion beam-scanning electron microscope (FIB-SEM) sample preparation techniques combined with TEM imaging to determine the track density/exposure age relations for lunar rock 64455. The 64455 sample was used earlier by [2] to determine a track production rate by chemical etching of tracks in anorthite. Here, we show that combined FIB/TEM techniques provide a more accurate determination of a track production rate and also allow us to extend the calibration to solar flare tracks in olivine.

Berger, Eve L.↗

NASA NTRS2003

Autotrophic Ecosystems on the Early Earth

Ophiolite sequences, sections of lower oceanic crust and upper mantle that have been thrust onto continental craton, are located in northern and central California and provide easily accessible areas that serve as good analogs for similar, more extensive areas of the early Earth. We have begun investigating and characterizing these sites in order to understand better the processes that may be responsible for the water chemistry, mineralogy and biology of similar environments on the early Earth. The geophysical and geochemical processes in these terranes provide niches for unique communities of extremeophiles and likely provide a good analog to the location that first gave rise to life on Earth. The ophiolites found in northern and central California include the Trinity, Josephine, Coast Range and Point Sal, all of which are approximately 160 million years old. Fluids from serpentinizing springs are generally alkaline with high pH and H2 contents, indicating that the mafic rock compositions control the fluid composition through water-rock reactions during relatively low-grade hydrothermal processes. There are significant amounts of primary mineralogy remaining in the rocks, meaning that substantial alteration processes are still occurring in these terranes. The general reaction for serpentinization of olivine is given by one of the authors. olivine + H2O = serpentine + brucite + magnetite + H2. We have analyzed the mineralogical composition of several rock samples collected from the Coast Range Ophiolite near Clear Lake, CA by electron microprobe. The remnant primary mineralogy is fairly urnform in composition, with an olivine composition of Fo(sub 90), and with pyroxene compositions of En(sub 90) for orthopyroxene and En(sub 49)Wo(sub 48)Fs(sub 03) for the clinopyroxene. Other primary phases observed include chromites and other spinels. Examination of petrographic thin sections reveals that serpentinization reactions have occurred in these locations. The serpentine resulting from aqueous alteration of olivine resides in veins that are see to cross cut the primary mineral grains. There are several generations of alteration products, comprised mostly of serpentines that are magnesium rich, with magnetite, brucite and carbonates observed as accessory minerals. The formation of carbonates can be taken to indicate the presence of CO2 in the altering fluids. We collected samples from a spring in the Coast Range Ophiolite in order to determine whether the geochemical environment serves as a habitat for chemotrophic microorganisms. DNA was extracted from the sediment samples and the 16s rRNA gene was PCR amplified using universal Archaeal primers. Denaturing gradient gel electrophoresis (DGGE) was used to determine the community of Archaea thriving in these samples. Our results indicate that there were 8 different genera of Archaea from a single sample. A sequence was obtained from one of these eight. The sequence is of an organism similar to Halorubrum tibetense, and alkalophilic Archaeon. This result suggests that these environments are likely hosts for communities of organisms that are adapted for the unique chemistry provided by the alkaline spring.

Schulte, M.↗

NASA NTRS1974

The electrical properties of low-g processed immiscible alloys

When dispersed or mixed immiscibles are solidified on earth, a large amount of separation of the constituents takes place due to differences in densities. However, when the immiscibles are dispersed and solidified in zero-gravity, density separation does not occur, and unique composite solids can be formed with many new and promising electrical properties. By measuring the electrical resistivity and superconducting critical temperature of zero-g processed Ga-Bi samples, it has been found that the electrical properties of such materials are entirely different from the basic constituents and the ground control samples. The finest dispersions are obtained only in a low-gravity environment. Our results indicate that space-processed immiscible materials may form an entirely new class of electronic materials. The interesting electrical properties of directionally solidified Al-Cu eutectic, a two-component alloy consisting of Al and Al2Cu which was processed on Skylab (M566) are briefly reviewed. The electrical resistivity of directionally solidified eutectics with a lamellar structure are highly anisotropic and have been studied both experimentally and theoretically. The local resistivity of the space-processed samples are compared to a ground control sample and to the expected theoretical results.

Otto, G. H.↗

NASA NTRS2010

Operational Lessons Learned from NASA Analog Missions

National Aeronautics and Space Administration s (NASA) efforts in human space flight are currently focused on the Space Shuttle and International Space Station (ISS) programs, with efforts beginning on the future exploration opportunities. Both the Space Shuttle and ISS programs are important to the development of a capability for human exploration beyond Low Earth Orbit (LEO). The ISS provides extensive research capabilities to determine how the human body reacts to long duration stays in space. Also, the ISS and Shuttle can serve as a limited testbed for equipment or entire systems that may be used on missions to the Moon, Mars, or to a near-Earth asteroid. It has been nearly 35 years since the Apollo astronauts visited the Moon. Future space explorers will have to re-learn how to work and live on planetary surfaces, and how to do that for extended periods of time. Exploration crews will perform a wide assortment of scientific tasks, including material sampling and emplacement of automated instruments. Surface mission operations include the activities of the crew living and working, mission support from the Earth, and the operation of robotic and other remotely commanded equipment on the surface and in planetary orbit. Other surface activities will include the following: exploring areas surrounding a habitat; using rovers to collect rock and soil samples; setting up experiments on the surface to monitor the radiation environment and any seismic or thermal activity; and conducting scientific analyses and experiments inside a habitat laboratory. Of course, the astronauts will also have to spend some of their surface time "doing chores" and maintaining their habitat and other systems. In preparation for future planetary exploration, NASA must design the answers to many operational questions. What will the astronauts do on the surface? How will they accomplish this? What tools will they require for their tasks? How will robots and astronauts work together? What vehicle and system capabilities are required to support the activities? How will the crew and the Earth-based mission control team interact? During the initial phases of manned planetary exploration, one challenge in particular is virtually the same as during the Apollo program: How can scientific return be maximized during a relatively short surface mission? Today, NASA is investigating solutions to these challenges by conducting analog missions. These Earth-based missions possess characteristics that are analogous to missions on the Moon or Mars. These missions are excellent for testing operational concepts, and the design, configuration, and functionality of spacesuits, robots, rovers, and habitats. Analog mission crews test specific techniques and procedures for surface field geology, biological sample collection, and planetary protection. The process of actually working an analog mission reveals a myriad of small details, which either contribute to or impede efficient operations, many of which would never have been thought about otherwise. It also helps to define the suite of tools, containers, and other small equipment that surface explorers will use. This paper focuses on how analog missions have addressed selected operational considerations for future planetary missions.

Arnold, Larissa S.↗

NASA NTRS2011

New Insights into Prebiotic Chemistry from Old Archived Miller Extracts

Following the discovery of an archived set of samples from Stanley Miller's early experiments, analyses were undertaken to better understand the diversity of compounds produced from electric discharges acting on reducing gas mixtures. The paper chromatography methods that Miller used in the 1950s were only capable of detecting a few amino acids and were unable to provide substantial quantitative data relative to today's techniques. Current analytical techniques are much more sensitive and selective, and are capable of precisely quantifying a much larger range of amino acids and their enantiomeric abundances. In one study, preserved dried samples produced by Miller using a lesser-known volcanic apparatus which differed from Miller's classic apparatus in that it utilized an aspirator that injected steam into the electric discharge chamber, simulating a volcanic eruption. The volcanic apparatus produced a wider variety of amino acids than the classic configuration. Prebiotic compounds synthesized in these environments may have locally accumulated where they could have undergone further processing. An additional preserved set of samples from an experiment conducted in 1958 were also found in Miller's archived collection. These samples which had been generated using a mixture of CH4, NH3, H2S and CO2 were collected, catalogued, and stored by Miller, but for unknown reasons were never studied. In our analyses a total of 23 amino acids and 4 amines, including 7 organosulfur compounds, were detected in these samples. The major amino acids with chiral centers are racemic within the accuracy of the measurements, indicating that they are not contaminants introduced during sample storage. This experiment marks the first synthesis of sulfur amino acids from spark discharge experiments designed to imitate primordial environments. The relative yield of some amino acids, in particular the isomers of amino butyric acid, are the highest ever found in a spark discharge experiment. The simulated primordial conditions used by Miller in these experiments may serve as a model for early volcanic plume chemistry and provide insight to the possible roles such plumes may have played in abiotic organic synthesis. Additionally, the overall abundances of the synthesized amino acids in the presence of H2S are very similar to the abundances found in some carbonaceous meteorites, suggesting that H2S may have played an important role in prebiotic reactions in early solar system environments. Although experiments using a variety of gases as components of the primordial Earth's atmospheric composition and a spark discharge apparatus configured according to Miller's original or volcanic design can be readily carried out, the unique opportunity to investigate samples prepared by the pioneer in abiotic synthesis using state of the art analytical methods is of considerable historic interest.

Parker, Eric T.↗

DOE OSTI2017

Resonance Self-shielding Impact on Neutron Spectrum Determination for Missouri S and T Reactor

Neutron Activation Analysis is a useful technique for characterizing the neutron flux at nuclear facilities. It involves irradiating samples to produce unstable isotopes, which enables qualitative and quantitative analyses of unknown samples. In the process, samples are bombarded with neutrons; new elements then become radioactive isotopes due to absorption of neutrons. These emit different amounts of gamma energy during the decay process, and gamma rays are counted using HPGe. Each reaction product has a known half-life and cross-section, and by measuring the amount of gamma radiation energy emitted from a radioactive isotope, it is possible to identify the isotope and activity. However, the neutron spectrum is not precise or representative of the actual neutron environment because of the self-shielding effect. This occurs with large samples that have large cross-sections or thicknesses in the resonance range of the neutron flux spectrum. Several methods have been used to mitigate this problem, such as thinning or diluting alloys samples, calculating the self-shielding factor for each material, and generating a corrected library for the unfolding procedure code. The Missouri University of Science and Technology Reactor (MSTR) is a pool-type research reactor that operates up to 200 kW. It uses light-water for both moderation and natural convective heat removal. The reactor core consists of 15 fuel elements positioned in a 9 x 6 aluminum grid plate, with four elements used for reactor power control. The fuel element is 19.75% low enriched uranium in 18 curved fuel plates. The reactor has seen various configurations starting with configuration designation 101W and subsequent reconfiguration increases the numeric designation by 1. The current configuration, 120W, was implemented in 2010. The new configuration provides more in-core locations for activation experiments and uses more fuel elements than previous ones. One of the irradiation locations in the MSTR core is the source holder. It is desired to improve the documentation of neutronic characteristics of the MSTR which are cardinal to experiments supported by the facility. One of these characteristics is the neutron spectrum at various locations in the reactor. Successful efforts yielded the neutron spectrum at a known location through the unfolding of the activity of irradiated foils. The purpose of this current study is to investigate the impact of resonance self-shielding of some of the activated foils on the determined spectrum.

21 SPECIFIC NUCLEAR REACTORS AND ASSOCIATED PLANTS↗

NASA NTRSDate not supplied

Continued Environmental Microbiology Monitoring of the International Space Station (ISS) Veggie Unit Used for In-Flight, Crop-Based Food Systems

Crewmembers live and work in a closed environment that is monitored to ensure their health and safety. To ensure occupants’ health and safety during their spaceflight residency, Environmental Health System (EHS) microbial samples including air, surface, and water, are collected, enumerated, and analyzed quarterly to monitor on-board system contamination and potential risks to crew health. Quarterly monitoring of the microorganisms in the ISS environment supports crew safety and contributes to a large set of microbial concentration and diversity data. Based upon data historically collected over the years, in-flight microbial requirements have been established to maintain the health and safety of the spacecraft environment. This study leverages quarterly operational Environmental Health System (EHS) sampling by collecting additional microbial samples from the surface of the station’s Veggie plant production system. Microbial surface samples collected from the Veggie plant production system will yield microbial concentration and diversity that can be compared and analyzed with nominal surface samples from the vehicle. The data collected in this study will aid in the development of requirements for spaceflight-based food production systems. Continued surface sampling of the internal and external surfaces of the Veggie locker, along with collaboration from both Johnson Space Center (JSC) & Kennedy Space Center (KSC) scientists studying the microbiome of the veggie-crop systems, will be implemented as part of the future development of crop-based food system requirements for the ISS and beyond. This presentation will include a review of the study procedures and evaluations of the current results.

Christian Mena↗

DOE OSTI2025

Field expedient stool collection methods for gut microbiome analysis in deployed military environments

ABSTRACT Field expedient devices and protocols for the collection, storage, and shipment of stool samples in deployed settings are needed for the advancement of microbiome research in military health. Relevant assessments include the evaluation of microbiome signatures associated with susceptibility to travelers’ diarrhea and recovery of gut function following infection. However, inherent biases in microbial measurements due to preservatives and sampling methods are unclear and should be assessed for an accurate evaluation of the microbiome. We performed shotgun metagenomic sequencing and compared the microbiome composition in paired fecal samples collected using Flinters Technology Associates (FTA) cards and OMNIgene (OG) Gut tubes, prior to and during international travel, from 49 adult participants, 39 of whom remained asymptomatic and 10 experienced travelers’ diarrhea. Higher concentrations of nucleic acid and sequencing libraries were observed in OG samples. A majority of genera (82.9%) were detected with both methods, and detections of genera limited to one collection method were not highly prevalent across samples and were present in extremely low relative abundances (<0.01%). Differences in beta diversity were largely explained by inter-individuality of microbiome composition, followed by the effect of collection method and timepoint-disease states. Differential abundance analysis indicated that Corynebacterium and Blautia were consistently higher in abundance across all groups with FTA and OG collection, respectively. The observed differences in microbiome composition between methods suggest the need for consistent and standardized protocols within a study. Overall, the data presented here could help guide the future design of fecal microbiome study protocols in field and military deployment settings. IMPORTANCE The assessment of field-deployable methods for fecal sample collection and storage is required to reliably capture samples collected in remote and austere locations. This study describes a comparative metagenomics analysis between samples collected by two different commercially available methods in a military-deployed setting. The results presented here are foundational for the future design of fecal microbiome study protocols in an operational context.

field study↗

NASA NTRS1992

Glass corrosion in natural environments

Experiments carried out during the progress period are summarized. Experiments carried out involving glass samples exposed to solutions of Tris have shown the appearance of 'spikes' upon monitoring glass dissolution as a function of time. The periodic 'spikes' observed in Tris-based media were interpreted in terms of cracking due to excessive stress in the surface region of the glass. Studies of the interactions of silicate glasses with metal ions in buffered media were extended to systems containing Al. Caps buffer was used to establish the pH. The procedures used are described and the results are given. Preliminary studies were initiated as to the feasibility of adding a slowly dissolving solid compound of the additive to the glass-water system to maintain a supply of dissolved additive. It appears that several magnesium compounds have a suitable combination of solubility and affinity towards silicate glass surfaces to have a pronounced retarding effect on the extraction of uranium from the glass. These preliminary findings raise the possibility that introducing a magnesium source into geologic repositories for nuclear waste glass in the form of a sparingly soluble Mg-based backfill material may cause a substantial reduction in the extent of long-term glass corrosion. The studies described also provide mechanistic understanding of the roles of various metal solutes in the leachant. Such understanding forms the basis for developing long-term predictions of nuclear waste glass durability under repository conditions. From what is known about natural highly reduced glasses such as tektites, it is clear that iron is dissolved as ferrous iron with little or no ferric iron. The reducing conditions were high enough to cause metallic iron to exsolve out of the glass in the form of submicroscopic spherules. As the nuclear waste glass is much less reduced, a study was initiated on other natural glasses in addition to the nuclear waste glass. Extensive measurements were carried out on these glasses in order to characterize their magnetic properties. Results of these studies are described.

Thorpe, Arthur N.↗

NASA NTRS2021

A sampling-based optimization approach to handling environmental uncertainty for a planetary lander

Planning for unknown environments presents a number of technical challenges. The planner must ensure robustness to unknown phenomena and manage unpredictable variation in execution, all while operating in a capacity that maximizes its objective. Productivity in the face of these challenges re-quires an integrated approach to planning and execution that is capable of accomplishing goals, reacting to variation, and maximizing overall utility. We examine this problem in the context of a Europa Lander concept mission. We model the problem as a hierarchical task network, framing it as a utility maximization problem constrained on a depletable energy resource. We propose an uncertainty–sensitive deterministic planning framework that utilizes periodic replanning to better handle model uncertainty and variable execution. We demonstrate the efficacy of our framework through simulations of a Europa Lander concept mission in which our algorithm out-performs several baseline approaches in both utility maximization and robustness

Zilberstein, Shlomo↗