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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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All sources NASA NTRS DOE OSTI

DOE OSTI2020

Minimally disruptive optical control of protein tyrosine phosphatase 1B

Protein tyrosine phosphatases regulate a myriad of essential subcellular signaling events, yet they remain difficult to study in their native biophysical context. Here we develop a minimally disruptive optical approach to control protein tyrosine phosphatase 1B (PTP1B)—an important regulator of receptor tyrosine kinases and a therapeutic target for the treatment of diabetes, obesity, and cancer—and we use that approach to probe the intracellular function of this enzyme. Our conservative architecture for photocontrol, which consists of a protein-based light switch fused to an allosteric regulatory element, preserves the native structure, activity, and subcellular localization of PTP1B, affords changes in activity that match those elicited by post-translational modifications inside the cell, and permits experimental analyses of the molecular basis of optical modulation. Findings indicate, most strikingly, that small changes in the activity of PTP1B can cause large shifts in the phosphorylation states of its regulatory targets.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2023

Interactions between fullerene derivatives and biological systems

Attention towards nanoparticles from the pharmaceutical and biomedical fields has significantly increased due to their attractive surface modification, high drug-loading, and improved pharmacokinetics. Fullerenes, an allotrope of carbon, stand out for their molecularly precise structure, potent radical-scavenging activity, photoactivatable reactive-oxygen species generation, and ability to definitively confine metal atoms and clusters. Accordingly, fullerene systems have been applied in various biological contexts, including increased and controlled drug delivery, antioxidative, anti-inflammatory, and photodynamic therapy, and magnetic resonance imaging. Ultimately, the pleiotropic activity of fullerenes, coupled with its precise structure and functionalization, can realize precise and tailorable medicines. Here, different from some excellent reviews focusing on the structure and chemistry of fullerene derivatives and their biomedical applications, this review highlights the interaction of fullerene materials with biological systems, with insights into their structural influence on their interactions with the cellular environment.

36 MATERIALS SCIENCE↗

DOE OSTI2025

Probing cold nuclear matter with energy correlators

The future electron-ion collider (EIC) will produce the first-ever high energy collisions between electrons and a wide range of nuclei, opening a new era in the study of cold nuclear matter. Quarks and gluons produced in these collisions will propagate through the dense nuclear matter of nuclei, imprinting its structure into subtle correlations in the energy flux of final state hadrons. In this article, we apply recent developments from the field of jet substructure, namely the energy correlator observables, to decode these correlations and provide a new window into nuclear structure. The energy correlators provide a calibrated probe of the scale dependence of vacuum quantum chromodynamics (QCD), enabling medium modifications to be imaged and interpreted as a function of scale. Using the eHIJING parton shower to simulate electron-nucleus collisions, we demonstrate that the size of the nucleus is imprinted as an angular scale in the correlators, with a magnitude that is visible for realistic EIC kinematics. We can observe the size difference between the proposed EIC nuclear targets 3 He, 4 He, 12 C, 40 Ca, 64 Cu, 197 Au, and 238 U, showing that the energy correlators can image femtometer length scales using asymptotic energy flux. Our approach offers a unified view of jet substructure across collider experiments, and provides numerous new theoretical tools to unravel the complex dynamics of QCD in extreme environments, both hot and cold.

Electron-ion collisions↗

DOE OSTI2025

NREL-AltDSS [SWR-25-58]

This repository builds upon the original work by PMeira at AltDSS-Schema, which aimed to define a structured JSON-based schema for DSS circuit representation. The schema has been packaged in this repository with slight modifications, enabling users to add the package as a dependency for downstream applications

Elgindy, Tarek [National Renewable Energy Laborato↗

DOE OSTI2019

STXM-XANES analyses of Murchison meteorite samples captured by aerogel after hypervelocity impacts: A potential implication of organic matter degradation for micrometeoroid collection experiments

The Tanpopo mission is an astrobiology space experiment at the Japanese Experiment Module (JEM) 'Kibo' on the International Space Station (ISS). One of the sub-divided themes of the Tanpopo mission is for the intact capture of organic bearing micrometeoroids in low Earth orbit using ultralow density silica aerogel (0.01 g/cm 3 ). In order to evaluate damage to organic matter in micrometeoroids during hyper velocity impacts into the aerogel, Murchison meteorite powdered samples, analogs of organic bearing micrometeoroids, were fired into flight-grade silica aerogel (0.01 g/cm 3 ) using a two-stage light-gas gun with velocities of 4.4 and 5.9 km/s. The recovered Murchison grains were analyzed using scanning transmission X-ray microscopy/X-ray absorption near edge structure (STXM/XANES), transmission electron microscopy (TEM) and nanoscale secondary ion mass spectrometry (NanoSIMS). TEM observation did not show significant modifications of the recovered Murchison grains. Carbon-XANES spectra, however, showed a large depletion of the organic matter after the 5.9 km/s impact, but no such effects nor any significant hydrogen isotopic fractionation were observed after the 4.4 km/s impact.

58 GEOSCIENCES↗

NASA NTRS1971

Single stage earth orbital reusable vehicle. Volume 6: Resources

The results of a conceptual study of the resource requirements for a single-stage earth-orbital vehicle are presented. All aspects of program costs for the design, manufacture, test, transportation, launch, and facility modifications were considered. The following program costs are discussed: configuration definition, cost groundrules and assumptions, program requirements, work breakdown structure, cost estimation methods, and cost analysis. High cost areas are identified.

Source record↗

NASA NTRS1982

Development tests for the 2.5 megawatt Mod-2 wind turbine generator

The 2.5 megawatt MOD-2 wind turbine generator test program is discussed. The development of the 2.5 megawatt MOD-2 wind turbine generator included an extensive program of testing which encompassed verification of analytical procedures, component development, and integrated system verification. The test program was to assure achievement of the thirty year design operational life of the wind turbine system as well as to minimize costly design modifications which would otherwise have been required during on site system testing. Computer codes were modified, fatigue life of structure and dynamic components were verified, mechanical and electrical component and subsystems were functionally checked and modified where necessary to meet system specifications, and measured dynamic responses of coupled systems confirmed analytical predictions.

Andrews, J. S.↗

NASA NTRS1982

Accomplishments of the NASA Johnson Space Center portion of the soil moisture project in fiscal year 1981

The NASA/JSC ground scatterometer system was used in a row structure and row direction effects experiment to understand these effects on radar remote sensing of soil moisture. Also, a modification of the scatterometer system was begun and is continuing, to allow cross-polarization experiments to be conducted in fiscal years 1982 and 1983. Preprocessing of the 1978 agricultural soil moisture experiment (ASME) data was completed. Preparations for analysis of the ASME data is fiscal year 1982 were completed. A radar image simulation procedure developed by the University of Kansas is being improved. Profile soil moisture model outputs were compared quantitatively for the same soil and climate conditions. A new model was developed and tested to predict the soil moisture characteristic (water tension versus volumetric soil moisture content) from particle-size distribution and bulk density data. Relationships between surface-zone soil moisture, surface flux, and subsurface moisture conditions are being studied as well as the ways in which measured soil moisture (as obtained from remote sensing) can be used for agricultural applications.

Paris, J. F.↗

NASA NTRS1988

Applying the OTV to lunar logistics

The Orbit Transfer Vehicle (OTV), representing the next generation of upper stages, has recently been studied in a Phase A concept definition study managed by NASA's Marshall Space Flight Center. The vehicle has been previously defined as strictly an orbit-to-orbit type transfer device. Recently its application to the task of lunar surface logistics was investigated. Transfer options to the surface were considered which included direct transfer, and transfer via lunar orbit as well as the L1 libration point. The subsystem modifications required to enable lunar landings were established for the following elements: aerobrake, main propulsion system, landing legs, primary structure, and avionics. It is concluded that the majority of the basic systems required for efficient transfer to the lunar surface are already contained in the OTV.

Willcockson, W. H.↗

NASA NTRS1993

Simulation of TunneLadder traveling-wave tube cold-test characteristics: Implementation of the three-dimensional, electromagnetic circuit analysis code micro-SOS

The three-dimensional, electromagnetic circuit analysis code, Micro-SOS, can be used to reduce expensive time-consuming experimental 'cold-testing' of traveling-wave tube (TWT) circuits. The frequency-phase dispersion characteristics and beam interaction impedance of a TunneLadder traveling-wave tube slow-wave structure were simulated using the code. When reasonable dimensional adjustments are made, computer results agree closely with experimental data. Modifications to the circuit geometry that would make the TunneLadder TWT easier to fabricate for higher frequency operation are explored.

Kory, Carol L.↗

NASA NTRS1977

Construction bidding cost of KSC's space shuttle facilities

The bidding cost of the major Space Transportation System facilities constructed under the responsibility of the John F. Kennedy Space Center (KSC) is described and listed. These facilities and Ground Support Equipment (GSE) are necessary for the receiving, assembly, testing, and checkout of the Space Shuttle for launch and landing missions at KSC. The Shuttle launch configuration consists of the Orbiter, the External Tank, and the Solid Rocket Boosters (SRB). The reusable Orbiter and SRB's is the major factor in the program that will result in lowering space travel costs. The new facilities are the Landing Facility; Orbiter Processing Facility; Orbiter Approach and Landing Test Facility (Dryden Test Center, California); Orbiter Mating Devices; Sound Suppression Water System; and Emergency Power System for LC-39. Also, a major factor was to use as much Apollo facilities and hardware as possible to reduce the facilities cost. The alterations to existing Apollo facilities are the VAB modifications; Mobile Launcher Platforms; Launch Complex 39 Pads A and B (which includes a new concept - the Rotary Service Structure), which was featured in ENR, 3 Feb. 1977, 'Hinged Space Truss will Support Shuttle Cargo Room'; Launch Control Center mods; External Tank and SRB Processing and Storage; Fluid Test Complex mods; O&C Spacelab mods; Shuttle mods for Parachute Facility; SRB Recovery and Disassembly Facility at Hangar 'AF'; and an interesting GSE item - the SRB Dewatering Nozzle Plug Sets (Remote Controlled Submarine System) used to inspect and acquire for reuse of SRB's.

Brown, Joseph Andrew↗

NASA NTRS2020

Design and Implementation of a Scenario Development Process for a 2040 Trajectory-Based Operations Simulation

The National Aeronautics and Space Administration (NASA) is supporting research to transition from a legacy, air traffic management (ATM) system to Trajectory-Based Operations (TBO) environment targeting the 2035-2045 timeframe by creating simulation scenarios for current and future studies. TBO in the National Airspace System (NAS) focuses on modernizing the current operating paradigm to increase efficiency, predictability, resilience, and flexibility while migrating toward greater operational autonomy across the airspace. These simulation scenarios offer a wide dissemination and use of system-level constraints and aircraft state/intent data, routine use of data communications to request complex trajectory modifications and to receive clearances, and aircraft that are flying on 4-D trajectories with flexibility when desired, but structure where required. This report describes the end-to-end development of scenarios for the NASA Langley Research Center simulation environment to represent a 2040 Trajectory-Based Operations airspace for air transport operations research.

Trajectory-Based Operations↗

DOE OSTI2024

Protein N -Glycans in Healthy and Sclerotic Glomeruli in Diabetic Kidney Disease

Diabetes is expected to directly affect renal glycosylation; yet to date, there has not been a comprehensive evaluation of alterations in N-glycan composition in the glomeruli of patients with diabetic kidney disease (DKD). Here, we used untargeted mass spectrometry imaging to identify N-glycan structures in healthy and sclerotic glomeruli in formalin-fixed paraffin-embedded sections from needle biopsies of five patients with DKD and three healthy kidney samples. Regional proteomics was performed on glomeruli from additional biopsies from the same patients to compare the abundances of enzymes involved in glycosylation. Secondary analysis of single-nucleus RNA sequencing (snRNAseq) data were used to inform on transcript levels of glycosylation machinery in different cell types and states. We detected 120 N-glycans, and among them, we identified 12 of these protein post-translated modifications that were significantly increased in glomeruli. All glomeruli-specific N-glycans contained an N-acetyllactosamine epitope. Five N-glycan structures were highly discriminant between sclerotic and healthy glomeruli. Sclerotic glomeruli had an additional set of glycans lacking fucose linked to their core, and they did not show tetra-antennary structures that were common in healthy glomeruli. Orthogonal omics analyses revealed lower protein abundance and lower gene expression involved in synthesizing fucosylated and branched N-glycans in sclerotic podocytes. In snRNAseq and regional proteomics analyses, we observed that genes and/or proteins involved in sialylation and N-acetyllactosamine synthesis were also downregulated in DKD glomeruli, but this alteration remained undetectable by our spatial N-glycomics assay. Integrative spatial glycomics, proteomics, and transcriptomics revealed protein N-glycosylation characteristic of sclerotic glomeruli in DKD.

60 APPLIED LIFE SCIENCES↗

DOE OSTI2022

The regulation of the cell wall by glycosylphosphatidylinositol-anchored proteins in Arabidopsis

A polysaccharides-based cell wall covers the plant cell, shaping it and protecting it from the harsh environment. Cellulose microfibrils constitute the cell wall backbone and are embedded in a matrix of pectic and hemicellulosic polysaccharides and glycoproteins. Various environmental and developmental cues can regulate the plant cell wall, and diverse glycosylphosphatidylinositol (GPI)-anchored proteins participate in these regulations. GPI is a common lipid modification on eukaryotic proteins, which covalently tethers the proteins to the membrane lipid bilayer. Catalyzed by a series of enzymic complexes, protein precursors are post-translationally modified at their hydrophobic carboxyl-terminus in the endomembrane system and anchored to the lipid bilayer through an oligosaccharidic GPI modification. Ultimately, mature proteins reach the plasma membrane via the secretory pathway facing toward the apoplast and cell wall in plants. In Arabidopsis, more than three hundred GPI-anchored proteins (GPI-APs) have been predicted, and many are reported to be involved in diverse regulations of the cell wall. In this review, we summarize GPI-APs involved in cell wall regulation. GPI-APs are proposed to act as structural components of the cell wall, organize cellulose microfibrils at the cell surface, and during cell wall integrity signaling transduction. Besides regulating protein trafficking, the GPI modification is potentially governed by a GPI shedding system that cleaves and releases the GPI-anchored proteins from the plasma membrane into the cell wall.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2023

Enzymes in 3D: Synthesis, remodelling, and hydrolysis of cell wall (1,3;1,4)-β-glucans

Abstract Recent breakthroughs in structural biology have provided valuable new insights into enzymes involved in plant cell wall metabolism. More specifically, the molecular mechanism of synthesis of (1,3;1,4)-β-glucans, which are widespread in cell walls of commercially important cereals and grasses, has been the topic of debate and intense research activity for decades. However, an inability to purify these integral membrane enzymes or apply transgenic approaches without interpretative problems associated with pleiotropic effects has presented barriers to attempts to define their synthetic mechanisms. Following the demonstration that some members of the CslF sub-family of GT2 family enzymes mediate (1,3;1,4)-β-glucan synthesis, the expression of the corresponding genes in a heterologous system that is free of background complications has now been achieved. Biochemical analyses of the (1,3;1,4)-β-glucan synthesized in vitro, combined with 3-dimensional (3D) cryogenic-electron microscopy and AlphaFold protein structure predictions, have demonstrated how a single CslF6 enzyme, without exogenous primers, can incorporate both (1,3)- and (1,4)-β-linkages into the nascent polysaccharide chain. Similarly, 3D structures of xyloglucan endo-transglycosylases and (1,3;1,4)-β-glucan endo- and exohydrolases have allowed the mechanisms of (1,3;1,4)-β-glucan modification and degradation to be defined. X-ray crystallography and multi-scale modeling of a broad specificity GH3 β-glucan exohydrolase recently revealed a previously unknown and remarkable molecular mechanism with reactant trajectories through which a polysaccharide exohydrolase can act with a processive action pattern. The availability of high-quality protein 3D structural predictions should prove invaluable for defining structures, dynamics, and functions of other enzymes involved in plant cell wall metabolism in the immediate future.

59 BASIC BIOLOGICAL SCIENCES↗

NASA NTRS2008

Using Composite Materials in a Cryogenic Pump

Several modifications have been made to the design and operation of an extended-shaft cryogenic pump to increase the efficiency of pumping. In general, the efficiency of pumping a cryogenic fluid is limited by thermal losses which is itself caused by pump inefficiency and leakage of heat through the pump structure. A typical cryogenic pump includes a drive shaft and two main concentric static components (an outer pressure containment tube and an intermediate static support tube) made from stainless steel. The modifications made include replacement of the stainless-steel drive shaft and the concentric static stainless-steel components with components made of a glass/epoxy composite. The leakage of heat is thus reduced because the thermal conductivity of the composite is an order of magnitude below that of stainless steel. Taking advantage of the margin afforded by the decrease in thermal conductivity, the drive shaft could be shortened to increase its effective stiffness, thereby increasing the rotordynamic critical speeds, thereby further making it possible to operate the pump at a higher speed to increase pumping efficiency. During the modification effort, an analysis revealed that substitution of the shorter glass/epoxy shaft for the longer stainless-steel shaft was not, by itself, sufficient to satisfy the rotordynamic requirements at the desired increased speed. Hence, it became necessary to increase the stiffness of the composite shaft. This stiffening was accomplished by means of a carbon-fiber-composite overwrap along most of the length of the shaft. Concomitantly with the modifications described thus far, it was necessary to provide for joining the composite-material components with metallic components required by different aspects of the pump design. An adhesive material formulated specially to bond the composite and metal components was chosen as a means to satisfy these requirements.

Batton, William D.↗

DOE OSTI2017

Structure of aryl O -demethylase offers molecular insight into a catalytic tyrosine-dependent mechanism

Significance Modern industrial and agricultural practices generate large quantities of aromatic pollutants; however, these waste products can be converted into fine chemicals, fuels, and plastics through biocatalytic pathways. The bacterial world can inform such utilization strategies as certain strains of soil and marine bacteria metabolize environmentally derived aromatics. Many of these metabolic pathways involve aryl intermediates that require demethylation to facilitate modification and ring opening for assimilation into the tricarboxylic acid (TCA) cycle. Aryl demethylases, which catalyze this reaction, are poorly understood, making their utilization in biotechnology difficult. We provide the structural and mechanistic characterization of a single-domain aryl demethylase, LigM, which employs a tyrosine-dependent mechanism. Insights from this work will inform synthetic biology approaches to convert underutilized aromatics into higher value compounds.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2017

Structural basis of divergent cyclin-dependent kinase activation by Spy1/RINGO proteins

Cyclin-dependent kinases (Cdks) are principal drivers of cell division and are an important therapeutic target to inhibit aberrant proliferation. Cdk enzymatic activity is tightly controlled through cyclin interactions, posttranslational modifications, and binding of inhibitors such as the p27 tumor suppressor protein. Spy1/RINGO (Spy1) proteins bind and activate Cdk but are resistant to canonical regulatory mechanisms that establish cell-cycle checkpoints. Cancer cells exploit Spy1 to stimulate proliferation through inappropriate activation of Cdks, yet the mechanism is unknown. We have determined crystal structures of the Cdk2-Spy1 and p27-Cdk2-Spy1 complexes that reveal how Spy1 activates Cdk. We find that Spy1 confers structural changes to Cdk2 that obviate the requirement of Cdk activation loop phosphorylation. Spy1 lacks the cyclin-binding site that mediates p27 and substrate affinity, explaining why Cdk-Spy1 is poorly inhibited by p27 and lacks specificity for substrates with cyclin-docking sites. We identify mutations in Spy1 that ablate its ability to activate Cdk2 and to proliferate cells. Our structural description of Spy1 provides important mechanistic insights that may be utilized for targeting upregulated Spy1 in cancer.

59 BASIC BIOLOGICAL SCIENCES↗