Final Technical Report, Comport: Rigorous Testing Methods to Safeguard Software Porting
Final report for Comport grant.
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Final report for Comport grant.
Urbanization is transforming landscapes globally, altering environmental conditions that affect ecosystem functioning, particularly in urban areas where trees are crucial for regulating microclimates, improving air quality, and sustaining biodiversity. This study investigates the environmental differences and tree leaf structure and morphology in urban and suburban sites in the Chicago Metropolitan Region. The leaf functional traits of Norway Maple and Little − leaved Linden were studied in three locations in the summer of 2023: an urban park (University of Illinois Chicago, Chicago, IL), a suburban park (Morton Arboretum, Lisle, IL), and a suburban residential site (Lombard, IL). The urban site had higher daytime and nighttime air, and land surface temperatures compared to the suburban sites with significant fluctuations observed across the sites. Cumulative growing degree days, a measure of potential photosynthetically active days, were also higher in the urban park than in the suburban sites between March and August. Norway Maple trees growing in the urban site displayed higher specific leaf area (SLA) and lower leaf dry matter content (LDMC) than in the suburban sites, resulting in thinner leaves. Similarly, Little−leaved Linden trees in the suburban residential site displayed higher SLA and lower LDMC than those in the suburban park. The values of gas exchange traits − namely photosynthetic assimilation, transpiration rates, and stomatal conductance − of Norway Maple were higher at the urban site compared to suburban sites as temperatures increased during the summer. Norway Maple gas exchange values decreased as the growing season progressed, as expected by ontogeny. In contrast, Little−leaved Linden maintained similar leaf gas exchange values throughout the growing season. Both species in the urban site exhibited lower instantaneous water use efficiency and reduced LDMC, suggesting greater water loss in response to elevated temperatures compared to suburban park and residential sites. Comparisons with existing global trait databases emphasize the need for localized data to accurately capture site−specific responses. Although some traits aligned with database values, others deviated significantly, underscoring the importance of comprehensive, site−specific datasets for robust ecosystem modeling and management strategies.
Electrocatalytic water splitting is a possible route to the expanded generation of green hydrogen; however, a long-term challenge is the requirement of fresh water as an electrolyzer feed. The use of seawater as a direct feed for electrolytic hydrogen production would alleviate fresh water needs and potentially open an avenue for locally generated hydrogen from marine hydrokinetic or off-shore power sources. One environmental limitation to seawater electrolysis is the generation of chlorine as a competitive anodic reaction. This work evaluates transition metal (W, Co, Fe, Sn, and Ru) doping of Mn-Mo-based catalysts as a strategy to suppress chlorine evolution while sustaining catalytic efficiency. Electrochemical evaluations in neutral chloride solution and raw seawater showed the promise of a novel Mn-Mo-Ru electrode system for oxygen evolution efficiency and enhanced catalytic activity. Subsequent stability testing in a flowing raw seawater flume highlighted the need for improved catalyst stability for long-term applications of Mn-Mo-Ru catalysts. This work highlights that elements known to be selective toward chlorine evolution in simple oxide form (e.g., RuO2) may display different trends in selectivity when used as isolated dopants, where Ru suppressed chlorine evolution in Mn-based catalysts.
Approximately 40% of the total U.S. office floor space of 1.5 billion sq.m (16 billion sq.ft.) is leased space occupied by tenants. Tenant fit-out presents a key opportunity to incorporate energy efficiency within the real estate business cycle. We designed a package of energy efficiency measures tailored to the scope of a tenant fit-out. This tenant fit-out package (TFP) includes advanced lighting and heating, ventilating and air-conditioning (HVAC) controls as core measures, with ceiling fans, automated shading, and plug load controls as additional optional measures. We conducted laboratory testing of six configurations of the package to evaluate energy savings, indoor environmental quality, and identify installation, commissioning, and operational issues. Combined savings for HVAC, lighting, and plug loads ranged from 33–40%. Lighting savings ranged from 69–83%, and HVAC savings from 20–40%. The laboratory testing also revealed some minor but tractable challenges with installation and commissioning of HVAC controls. Overall, the results demonstrate that significant savings can be realized in existing office buildings by incorporating relatively low-risk, proven measures at the time of a tenant fit-out.
We report on a seventh annual workshop in a series focused on science realized by the combination of hard X-ray free electron lasers with high power optical lasers, hosted at the SLAC National Accelerator Laboratory in Menlo Park, CA. Members from the user community of the Matter in Extreme Conditions (MEC) endstation of the Linac Coherent Light Source (LCLS) and other scientists met with local scientists to discuss developments at LCLS and MEC and related facilities, including experimental results and future plans.
Aspergillus terreus has attracted interest due to its application in industrial biotechnology, particularly for the production of itaconic acid and bioactive secondary metabolites. As related species also seem to possess a prosperous secondary metabolism, they are of high interest for genome mining and exploitation. Here, we present draft genome sequences for six species from Aspergillus section Terrei and one species from Aspergillus section Nidulantes. Whole-genome phylogeny confirmed that section Terrei is monophyletic. Genome analyses identified between 70 and 108 key secondary metabolism genes in each of the genomes of section Terrei, the highest rate found in the genus Aspergillus so far. The respective enzymes fall into 167 distinct families with most of them corresponding to potentially unique compounds or compound families. Moreover, 53% of the families were only found in a single species, which supports the suitability of species from section Terrei for further genome mining. Intriguingly, this analysis, combined with heterologous gene expression and metabolite identification, suggested that species from section Terrei use a strategy for UV protection different to other species from the genus Aspergillus. Section Terrei contains a complete plant polysaccharide degrading potential and an even higher cellulolytic potential than other Aspergilli, possibly facilitating additional applications for these species in biotechnology.
Background: HIV-1 envelope (Env) variable loops 1 and 2 (V1V2) directed non-neutralizing antibodies were a correlate of decreased transmission risk in the RV144 vaccine trial. Thus, the elicitation and breadth of antibody responses against the V1V2 of HIV-1 Env are important considerations for HIV-1 vaccine candidates. The V1V2 region’s highly variable nature and the extensive diversity of subtype C HIV-1 Envelopes (Envs) make the V1V2 response breadth a high priority for HIV-1 vaccine regimens aiming for V1V2-mediated protection in Southern Africa. Here, we determined whether the breadth of the anti-V1V2 vaccine response can be broadened by including HIV-1 Env strains computationally designed to enhance the coverage of subtype C V1V2 sequence diversity. Methods: Three subtype C Env strains were selected to maximize antibody binding coverage while complementing subtype C vaccine gp120s that were given in human clinical trials in South Africa, as well as to improve epitope accessibility. Humoral immunogenicity of a novel trivalent gp120 vaccine immunogen, a bivalent gp120 boost already in clinical trials (1086C and TV1), and a pentavalent (all five gp120s combined) were evaluated in a preclinical immunization study in guinea pigs. The pentavalent combination was further evaluated with alum versus glucopyranosyl lipid adjuvants formulated in squalene-in-water emulsion (GLA-SE) adjuvants in non-human primates. The breadth of the anti-V1V2 response was assessed using an array of cross-subtype variable loops 1&2 (V1V2) scaffold proteins and linear V2 peptides. Results: The breadth of the IgG response against V1V2 antigens of the trivalent and pentavalent groups was comparable, and both were greater than the breadth of the bivalent group. Linear epitope mapping showed that two linear epitopes in V2 were targeted by the vaccinated animals: the V2 hotspot focused at 169K that potentially correlated with decreased HIV-1 risk in RV144 and the V2.2 site (179LDV/I181) that is part of the integrin α4β7 binding site. The bivalent vaccine elicited a significantly higher magnitude of binding to the V2 hotspot compared to the trivalent vaccine whereas the trivalent vaccine elicited significantly higher binding to the V2.2 epitope compared to the bivalent vaccine, while the pentavalent recognized both regions. Conclusions: These results demonstrate that the three new computationally selected subtype C Envs successfully complemented 1086C and TV1 for broader V1V2 antibody responses, and, in concert with adjuvants that stimulate V1V2 responses, can be considered as part of a rationale immunogen design to improve V1V2 IgG coverage in future vaccine trials in South Africa.
Abstract. The large spatial scale of global Earth system models (ESMs) is often cited as an obstacle to using the output by water resource managers in localized decisions. Recent advances in computing have improved the fidelity of hydrological responses in ESMs through increased connectivity between model components. However, the models are seldom evaluated for their ability to reproduce metrics that are important for and resonate with practitioners or that allow practitioners to situate higher-resolution model outputs within a cascade of uncertainty stemming from different models and scenarios. We draw on the combined experience of the author team and water manager workshop participants to identify salient water management metrics and evaluate whether they are credibly reproduced over the conterminous USA by the Community Earth System Model v2 (CESM2) Large Ensemble. We find that, while the exact values may not match the observations, aspects such as interannual variability can be reproduced by CESM2 for the mean wet day precipitation and length of dry spells. CESM2 also captures the proportion of total annual precipitation that derives from the heaviest rain days in watersheds that are not snow-dominated. Aggregating the 7 d mean daily runoff to two-digit Hydrological Unit Code (HUC2) watersheds also shows that rain-dominated regions capture the timing and interannual variability of annual maximum and minimum flows. We conclude that there is potential for far greater use of large-ensemble ESMs, such as CESM2, in long-range water management decisions to supplement high-resolution regional projections.
Compiler optimizations can alter significantly the numerical results of scientific computing applications. When numerical results differ significantly between compilers, optimization levels, and floating-point hardware, these numerical inconsistencies can impact programming productivity. pLiner is a framework that helps programmers identify locations in the source code that are highly affected by compiler optimizations. pLiner uses a novel approach to identify such code locations by enhancing the floatingpoint precision of variables and expressions. Using a guided search to locate the most significant code regions, pLiner can report to users such locations at different granularities, file, function, and line of code.
Redox reactions are central to biochemistry and are both controlled by and induce protein structural changes. Here, we describe structural rearrangements and crosstalk within the Bacillus cereus ribonucleotide reductase R2b–NrdI complex, a di-metal carboxylate-flavoprotein system, as part of the mechanism generating the essential catalytic free radical of the enzyme. Femtosecond crystallography at an X-ray free electron laser was utilized to obtain structures at room temperature in defined redox states without suffering photoreduction. Together with density functional theory calculations, we show that the flavin is under steric strain in the R2b–NrdI protein complex, likely tuning its redox properties to promote superoxide generation. Moreover, a binding site in close vicinity to the expected flavin O 2 interaction site is observed to be controlled by the redox state of the flavin and linked to the channel proposed to funnel the produced superoxide species from NrdI to the di-manganese site in protein R2b. These specific features are coupled to further structural changes around the R2b–NrdI interaction surface. The mechanistic implications for the control of reactive oxygen species and radical generation in protein R2b are discussed
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A model to be used in the flow studies and curved Thermal Protection System (TPS) evaluations was tested in the Langley 8 Foot High-Temperature Tunnel at a nominal Mach number of 6.8. The purpose of the study was to define the surface pressure and heating rates at high angles of attack (in support of curved metallic TPS studies) and to determine the conditions for which the model would be suitable as a test bed for aerothermal load studies. The present study was conducted at a nominal total temperature of 2400 and 3300 R, dynamic pressures from 2.3 to 10.9 psia, and free-stream Reynolds numbers from 4000,000 to 1,700,000/ft. The measurements consisted primarily of surface pressure and cold-wall (530 R) heating rates. Qualitative comparisons between predictions and data show that for this configuration, aerothermal tests should be limited to angles of attack between 10 and -10 degrees. Outside this range, the effects of free-stream flow nonuniformity appear in the data, as a result of the long length of the model. However, for TPS testing, this is not a concern and tests can be performed at angles of attack ranging from 20 to -20 degrees. Laminar and naturally turbulent boundary layers are available over limited ranges of conditions.
Experiments were conducted at NASA Ames Research Center to define multi-tasking software requirements for multiple-instruction, multiple-data stream (MIMD) computer architectures. The focus was on specifying solutions for algorithms in the field of computational fluid dynamics (CFD). The program objectives were to allow researchers to produce usable parallel application software as soon as possible after acquiring MIMD computer equipment, to provide researchers with an easy-to-learn and easy-to-use parallel software language which could be implemented on several different MIMD machines, and to enable researchers to list preferred design specifications for future MIMD computer architectures. Analysis of CFD algorithms indicated that extensions of an existing programming language, adaptable to new computer architectures, provided the best solution to meeting program objectives. The CoFORTRAN Language was written in response to these objectives and to provide researchers a means to experiment with parallel software solutions to CFD algorithms on machines with parallel architectures.
Spectroscopic observations of H2 in specific longitude regions of Jupiter as they rotate from limb to limb are reported. Data obtained in the 3-0 S(0) and S(1) lines using a CCD detector and a spectrograph with either echelle or plane gratings at the Cassegrain focus of the 24-inch telescope at Whipple Observatory during April-June 1983 are presented in extensive tables and graphs and analyzed in detail, modeling spatial and temporal variations at seven latitudes. An east-to-west increase in the equivalent line widths is attributed to the combined action of internal and solar heating of a convective layer, resulting in diurnal changes in the vertical cloud structure. It is inferred that the hydrogen observed is mainly in an equilibrium thermodynamic state, with small amounts of nonequilibrium hydrogen at high altitudes.
The Space Station Freedom Solar Array Program is required to provide a 75 kW power module that uses eight solar array (SA) wings over a four-year period in low Earth orbit (LEO). Each wing will be capable of providing 23.4 kW at the 4-year design point. Lockheed Missles and Space Company, Inc. (LMSC) is providing the flexible substrate SAs that must survive exposure to the space environment, including atomic oxygen, for an operating life of fifteen years. Trade studies and development testing, important for evolving any design to maturity, are presently underway at LMSC on the flexible solar array. The trade study and development areas being investigated include solar cell module size, solar cell weld pads, panel stiffener frames, materials inherently resistant to atomic oxygen, and weight reduction design alternatives.
Automated power-distribution system monitors and controls electrical power to modules in network. Handles both 208-V, 20-kHz single-phase alternating current and 120- to 150-V direct current. Power distributed to load modules from power-distribution control units (PDCU's) via subsystem distributors. Ring busses carry power to PDCU's from power source. Needs minimal attention. Detects faults and also protects against them. Potential applications include autonomous land vehicles and automated industrial process systems.
Fluctuating pressures were measured beneath a Mach 5, turbulent boundary layer on a flat plate with an array of piezoresistive sensors. The data were obtained with a digital signal acquisition system during a test run of 4 seconds. Data sampling rate was such that frequency analysis up to 62.5 kHz could be performed. To assess in situ frequency response of the sensors, a specially designed waveguide calibration system was employed to measure transfer functions of all sensors and related instrumentation. Pressure time histories were approximated well by a Gaussian prohibiting distribution. Pressure spectra were very repeatable over the array span of 76 mm. Total rms pressures ranged from 0.0017 to 0.0046 of the freestream dynamic pressure. Streamwise, space-time correlations exhibited expected decaying behavior of a turbulence generated pressure field. Average convection speed was 0.87 of freestream velocity. The trendless behavior with sensor separation indicated possible systematic errors.
Robot dynamics and control will become an important issue for productive platforms in space. Robotic operations will be necessary for both man tended stations and for the efficient performance of routine operations in a manned platform. The current constraints on the use of robotic devices in a microgravity environment appears to be due to safety concerns and an anticipated increase in acceleration levels due to manipulator motion. The robot used for the initial studies was a UMI RTX robot, which was adapted to operate in a materials processing workcell to simulate sample changing in a microgravity environment. The robotic cell was flown several times on the KC-135 aircraft at Ellington Field. The primary objective of the initial flights was to determine operating characteristics of both the robot and the operator in the variable gravity of the KC-135 during parabolic maneuvers. It was demonstrated that the KC-135 aircraft can be used for observing dynamics of robotic manipulators. The difficulties associated with humans performing teleoperation tasks during varying G levels were also observed and can provide insight into some areas in which the use of artificial techniques would provide improved system performance. Additionally a graphic simulation of the workcell was developed on a Silicon Graphics Workstation using the IGRIP simulation language from Deneb Robotics. The simulation is intended to be used for predictive displays of the robot operating on the aircraft. It is also anticipated that this simulation can be useful for off-line programming of tasks in the future.