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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 163 records · Page 9
All sourcesNASA NTRSDOE OSTI
NASA NTRS2014

Micro-Fluidic Device for Drug Delivery

A microfluidic device is provided for delivering a drug to an individual. The microfluidic device includes a body that defines a reservoir for receiving the drug therein. A valve interconnects the reservoir to an output needle that is insertable into the skin of an individual. A pressure source urges the drug from the reservoir toward the needle. The valve is movable between a closed position preventing the flow of the drug from the reservoir to the output needle and an open position allowing for the flow of the drug from the reservoir to the output needle in response to a predetermined condition in the physiological fluids of the individual.

Beebe, David J.↗
NASA NTRS2023

Automated Fluidics Device for Extraction and Quantification of miRNA Biomarkers From Blood

Radiation Assessment DuRing Exposure And long-Duration Spaceflight (RADREADS) demonstrates space-compatible point-of-care technology for quantitative biological monitoring of blood miRNA biomarkers in response to long-term low dose radiation exposure. This individualized monitoring approach will inform targeted treatment strategies to maximize medical resource utilization by accounting for individual susceptibility to radiation-related illnesses. As human spaceflight progresses beyond Earth’s magnetic shielding, radiation exposure poses a significant risk to astronaut health and safety. Extended operation in this environment comes with an increased risk of radiation exposure, leading to higher risks of radiation sickness, cancer, central nervous system effects, and degenerative diseases. While conventional physical dosimetry techniques capture radiation dose, individualistic susceptibility to radiation damage is varied. Multiple characteristics, including age, body weight, sex, genetics, and immune status, have been found to influence radiosensitivity (Liu et al. 2011, and Bouffler 2016). This differential response necessitates individualized monitoring and targeted treatment strategies to maximize medical resource utilization; however, a practical diagnostic platform for quantifying long-term, low dose radiation-induced tissue damage does not currently exist. MicroRNAs (miRNAs) are a class of small, non-coding RNAs that regulate gene expression by mediating the degradation of messenger RNA. The levels of particular miRNAs are influenced by biological processes such as inflammation and serve as biomarkers for a variety of conditions including cancer (Singh et al. 2017). MicroRNAs are found in various bodily fluids and are amenable to collection via liquid biopsies, providing a minimally invasive and easily quantifiable readout for a variety of radiosensitive reporters. A preliminary signature of 15 spaceflight sensitive miRNA has been identified in rodent and human studies, including miR-21-5p, miR-24-3p, miR-92a-3p, miR-17-5p, miR-16a-3p, miR-34a-3p, and miR-223-3p. These targets generally increased expression with radiation dose and linear energy transfer, though variation between individuals is not yet described. Current gaps in the field include a lack of understanding of longitudinal biological responses to long-term, low dose radiation exposure and the absence of space-compatible point-of-care technology for quantitative biological monitoring. In this body of work, we aim to develop an automated bleed-to-read system to process whole blood for the detection of miRNA biomarkers in order to monitor individualistic responses to radiation exposure. This will be achieved via separating serum (or plasma) from whole blood, followed by extraction, amplification, and quantification of the miRNA using a RT-qPCR reaction. Previously, the WetLab-2 hardware enabled execution of a RT-qPCR reaction aboard ISS; however, it is a manual system that requires crew manipulation and bulky components (Parra et al. 2017). To address these issues, automated fluid handling hardware was developed for each stage of sample preparation. Extraction of total RNA is achieved by sequentially pumping reagents through an off-the-shelf nucleic acid binding column (miRNeasy Serum/Plasma Advanced Kit, Qiagen). This approach eliminates several manual pipetting and centrifuging steps and limits the use of toxic chemicals commonly found in other sample processing techniques. The resulting elution will then be automatically dispensed for RT-qPCR analysis using a compact rotary qPCR (Mic qPCR Cycler, Bio Molecular Systems) that will improve spaceflight compatibility by removing bubbles from the detection region, another challenge highlighted by WetLab-2 (Parra et al. 2017). Efforts are also being made to simplify the RT-qPCR reaction to a 1-step air-dryable mix to improve long-term reagent stability at room temperature and reduce system complexity. By automating the RT-qPCR processes via microfluidic manipulation, RADREADS will reduce crewmember hands-on time and enable the personalized detection of radiation-induced tissue damage during long duration missions. Minimally invasive, longitudinal monitoring of individual’s response to radiation exposure will inform how the physiological system responds to long-term low dose space radiation and enables development of targeted countermeasures by the medical team. Ultimately, this portable technology will require minimal technical expertise and can also be used to monitor miRNA biomarkers associated with other diseases.

Tristen Head↗
NASA NTRS2012

A PIV Study of Slotted Air Injection for Jet Noise Reduction

Results from acoustic and Particle Image Velocimetry (PIV) measurements are presented for single and dual-stream jets with fluidic injection on the core stream. The fluidic injection nozzles delivered air to the jet through slots on the interior of the nozzle at the nozzle trailing edge. The investigations include subsonic and supersonic jet conditions. Reductions in broadband shock noise and low frequency mixing noise were obtained with the introduction of fluidic injection on single stream jets. Fluidic injection was found to eliminate shock cells, increase jet mixing, and reduce turbulent kinetic energy levels near the end of the potential core. For dual-stream subsonic jets, the introduction of fluidic injection reduced low frequency noise in the peak jet noise direction and enhanced jet mixing. For dual-stream jets with supersonic fan streams and subsonic core streams, the introduction of fluidic injection in the core stream impacted the jet shock cell structure but had little effect on mixing between the core and fan streams.

Henderson, Brenda S.↗
NASA NTRS2002

Propulsion Simulations with the Unstructured-Grid CFD Tool TetrUSS

A computational investigation has been completed to assess the capability of the NASA Tetrahedral Unstructured Software System (TetrUSS) for simulation of exhaust nozzle flows. Three configurations were chosen for this study: (1) a fluidic jet effects model, (2) an isolated nacelle with a supersonic cruise nozzle, and (3) a fluidic pitchthrust- vectoring nozzle. These configurations were chosen because existing data provided a means for measuring the ability of the TetrUSS flow solver USM3D for simulating complex nozzle flows. Fluidic jet effects model simulations were compared with structured-grid CFD (computational fluid dynamics) data at Mach numbers from 0.3 to 1.2 at nozzle pressure ratios up to 7.2. Simulations of an isolated nacelle with a supersonic cruise nozzle were compared with wind tunnel experimental data and structured-grid CFD data at Mach numbers of 0.9 and 1.2, with a nozzle pressure ratio of 5. Fluidic pitch-thrust-vectoring nozzle simulations were compared with static experimental data and structured-grid CFD data at static freestream conditions and nozzle pressure ratios from 3 to 10. A fluidic injection case was computed with the third configuration at a nozzle pressure ratio of 4.6 and a secondary pressure ratio of 0.7. Results indicate that USM3D with the S-A turbulence model provides accurate exhaust nozzle simulations at on-design conditions, but does not predict internal shock location at overexpanded conditions or pressure recovery along a boattail at transonic conditions.

Deere, Karen A.↗
NASA NTRS2015

Investigation of Spiral and Sweeping Holes

Surface infrared thermography, hotwire anemometry, and thermocouple surveys were performed on two new film cooling hole geometries: spiral/rifled holes and fluidic sweeping holes. The spiral holes attempt to induce large-scale vorticity to the film cooling jet as it exits the hole to prevent the formation of the kidney shaped vortices commonly associated with film cooling jets. The fluidic sweeping hole uses a passive in-hole geometry to induce jet sweeping at frequencies that scale with blowing ratios. The spiral hole performance is compared to that of round holes with and without compound angles. The fluidic hole is of the diffusion class of holes and is therefore compared to a 777 hole and Square holes. A patent-pending spiral hole design showed the highest potential of the non-diffusion type hole configurations. Velocity contours and flow temperature were acquired at discreet cross-sections of the downstream flow field. The passive fluidic sweeping hole shows the most uniform cooling distribution but suffers from low span-averaged effectiveness levels due to enhanced mixing. The data was taken at a Reynolds number of 11,000 based on hole diameter and freestream velocity. Infrared thermography was taken for blowing rations of 1.0, 1.5, 2.0, and 2.5 at a density ration of 1.05. The flow inside the fluidic sweeping hole was studied using 3D unsteady RANS.

infrared thermography↗
NASA NTRS2018

Toward Cooling Uniformity: Investigation of Spiral, Sweeping Holes, and Unconventional Cooling Paradigms

Surface infrared thermography, hotwire anemometry, and thermocouple surveys were performed on two new film cooling hole geometries: spiral/rifled holes and fluidic sweeping holes. Ways to quantify the efficacy of novel cooling holes that are asymmetric, not uniformly spaced or that show variation from hole to hole are presented. The spiral holes attempt to induce large-scale vorticity to the film cooling jet as it exits the hole to prevent the formation of the kidney shaped vortices commonly associated with film cooling jets. The fluidic sweeping hole uses a passive in-hole geometry to induce jet sweeping at frequencies that scale with blowing ratios. The spiral hole performance is compared to that of round holes with and without compound angles. The fluidic hole is of the diffusion class of holes and is therefore compared to a 777 hole and square holes. A patent-pending spiral hole design showed the highest potential of the nondiffusion type hole configurations. Velocity contours and flow temperature were acquired at discreet cross-sections of the downstream flow field. The passive fluidic sweeping hole shows the most uniform cooling distribution but suffers from low span-averaged effectiveness levels due to enhanced mixing. The data was taken at a Reynolds number of 11,000 based on hole diameter and freestream velocity. Infrared thermography was taken for blowing ratios of 1.0, 1.5, 2.0, and 2.5 at a density ratio of 1.05. The flow inside the fluidic sweeping hole was studied using 3D unsteady RANS. A section on ideas for future work is included that addresses issues of quantifying cooling uniformity and provides some ideas for changing the way we think about cooling such as changing the direction of cooling or coupling acoustic devices to cooling holes to regulate frequency.

Shyam, Vikram↗
NASA NTRSDate not supplied

The BioSensor Instrument Beyond LEIA: a Versatile Platform for Lunar Biology

Introduction: The BioSensor is a deep-space-compatible automated microfluidic culturing instrument. While originally designed to measure the effects of deep space radiation on yeast growth for the BioSentinel mission, it has the potential to host a diverse range of life science experiments with single- and/or multi-celled organisms and can be adapted to interface with a diverse range of platforms in both crewed and uncrewed settings. It is therefore a leading candidate for hosting life sciences experimentation associated with a lunar surface habitat. BioSensor design: The function of the BioSensor is to monitor the growth and metabolic activity of samples in batch fluidic culture, without the need for crew involvement. The current configuration houses organisms in 16 wells within 16 microfluidic cards, accommodating a total of 256 samples, replicates, and controls. Each well has an optical system including three LEDs and a photodiode detector to measure absorbance at three wavelengths, enabling measurement of optical density, color change in dyes such as alamarBlue, and bioproduction of pigmented compounds. Organisms are loaded into fluidic wells and air-dried for storage during integration, launch, and transit, then activated by the introduction of culture medium from storage bags via manifolds that fill one card at a time. Temperature is controlled by individual card heaters, and timing of all activities (fluidics fills, optical measurements, temperatures) is directed by an experiment script. The self-contained BioSensor payload is roughly 4U in volume; with electrical/mechanical/thermal interface, e.g. for operation on ISS or a lunar lander, as well as a linear energy-transfer (LET) charged-particle radiation spectrometer, the entire system is closer to 6U. BioSentinel and LEIA: Flown on the ISS and in a deep-space free flyer for BioSentinel, the BioSensor has been modified for use in the LEIA mission, including improvements to reduce the sensitivity to lengthy launch delays. LEIA will monitor yeast growth in the radiation and reduced-gravity environment of the lunar surface no earlier than 2026, on a CLPS lander [4]. Changes include accommodating additional culture media and an additional LED color for a new biological assay (bioproduction of carotenoids-- dietary antioxidants), as well as modifications to the housing to allow late-load biology changeout and improved isolation between fluidics and electronics. Future prospects: Future work with the BioSensor, beyond LEIA, will include expanding the range and diversity of organisms and assays accommodated. Preliminary work has demonstrated the growth of Arabidopsis seedlings in BioSensor fluidic cards, including optical measurements of growth rate over time. Minor modifications could allow measurement of phenotypes related to photosynthetic capacity in both plants and cyanobacteria. The experimental capabilities of the BioSensor could be dramatically increased by introducing the capability for fluorescence measurements, and/or the design of novel biological assays using luminescence. The BioSensor can also be adapted for new platforms and experiment settings; in addition to free-flyer, ISS, and CLPS lander, a preliminary design concept has been developed for crewed deployment directly to the lunar surface. The instrument could be accommodated inside a lunar habitat, where its automated operation would make it an excellent candidate for experiments from fundamental investigations into the response of organisms to lunar surface conditions to applied-science purposes such as screening engineered strains of various organisms for bioproduction capability.

J A Lee↗
NASA NTRS1974

Wind tunnel tests of a symmetrical airfoil with scoop fed slots

The design and wind tunnel test of a model vertical tail fin is described in this report. The model is designed to provide the aerodynamic forces necessary for lateral stabilization without moving parts or a separate source of power. It employs scoop-fed slots on both surfaces of the symmetrical airfoil. They are to be controlled differentially by means of a fluidic amplifier to implement an automatic fulltime lateral stabilization system. The results of tests show that the control of forces is stable and quite linear in various modes of operation. Significant forces were produced that can be increased as necessary by increasing slot size and scoop size. Slots can be located ahead of the conventional rudder and the scoop can be at the base of the vertical tail fin to avoid the need for major changes in conventional aircraft design. The first phase of the work demonstrated the feasibility of no-moving-parts aircraft control. The second phase established that a practical fluidic amplifier can be built to control slot flows from fluidic signals. Recommendations are made to optimize the design of the fluidic amplifier and to characterize its dynamic response in support of further analytical studies.

Belsterling, C. A.↗
NASA NTRS1990

Method and system for measuring sound velocity

A method and system for determining the speed of sound in a fluidic medium by determining the travel time of an acoustical signal a predetermined distance in a fluidic medium by generating a cyclical reference signal of a predetermined frequency and transmitting a portion of the reference signal through the medium. The transmitted portion of the reference signal is received after travelling a predetermined distance in the fluidic medium. The cycles of the cyclical reference signal are counted during the period of time between the transmitting and receiving of the portion of the reference signal wherein the travel time of the portion of the reference signal, is the number of cycle counts divided by the frequency. The speed of the acoustical signal through the fluidic medium is a function of the path length divided by the travel time.

Boegeman, Dwight E.↗
NASA NTRS2019

Active Flow Control on Vertical Tail Models

Active flow control (AFC) subscale experiments were conducted at the Lucas Wind Tunnel of the California Institute of Technology. Tests were performed on a generic vertical tail model at low speeds. Fluidic oscillators were used at the trailing edge of the main element (vertical stabilizer) to redirect the flow over the rudder and delay or prevent flow separation. Side force increases in excess of 50% were achieved with a 2% momentum coefficient (C(sub μ)) input. The results indicated that a collective C(sub μ) of about 1% could increase the side force by 30–50%. This result is achieved by reducing the spanwise flow on the swept back wings that contributes to early flow separation near their tips. These experiments provided the technical backdrop to test the full-scale Boeing 757 vertical tail model equipped with a fluidic oscillator system at the National Full-scale Aerodynamics Complex 40-by 80-foot Wind Tunnel, NASA Ames Research Center. The C(sub μ) is shown to be an important parameter for scaling a fluidic oscillator AFC system from subscale to full-scale wind tunnel tests. The results of these tests provided the required rationale to use a fluidic oscillator AFC configuration for a follow-on flight test on the Boeing 757 ecoDemonstrator.

Andino, Marlyn Y.↗
NASA NTRS2020

BioSentinel ISS Control Experiment Final Investigation Summary Form

Each payload has 18 fluidic cards and each fluidic card has 16 microfluidic wells, for a total of 288 wells. Card activation occurs at different time points throughout the 6-month mission and following ground commands. At each timepoint, two fluidic cards are filled with growth medium containing nutrients and a metabolic indicator dye. In addition to card filling, the temperature of the cards increases from ~4°C (stasis mode) to 23°C to allow for cell growth. The metabolic dye changes color from blue to pink, then pink to clearin the presence of metabolically active yeast cells. The colorimetric changes and cell growth are monitored using a 3-light-emitting diode(LED)optical detection system. The optical data determines how fast the cells are able to recover after accumulating radiation damage over time.After ground command initiation, all the fluidics, thermal and optical detection steps in BioSentinel are fully automated.The biosensor response data is compared to physical dosimetry data collected onboard the same payload. This dosimeter or linear energy transfer (LET)spectrometer is based on TimePix chip technology. This chip measures the total ionizing radiation dose in addition to calculating the LET of each particle that traverses its sensor, thus allowing researchers to characterize the radiation environment around the payload.The biological response (and physical dosimetry) on ISS over time is compared to the deep space mission and to data obtained on the ground.This analysis provides insight on the biological effects of the space radiation exposure in preparation for future long-duration missions to space and inhabitation beyond low-Earth orbit (LEO).

BioSentinel↗
NASA NTRSDate not supplied

Beyond BioSentinel: Iterative Development of Automated Microfluidics

NASA Ames has flown a series of Bio-CubeSats that performed biology experiments supported by automated fluidic systems. Since Genesat-1 in 2006, these payloads have increased in complexity and functionality, building upon previous successes, and applying lessons learned. BioSentinel was the most recent of this series, launched into heliocentric orbit onboard Artemis-1 in 2022. This presentation will discuss how the fluidic technology developed for these Bio-CubeSat missions, including the multi-layer polycarbonate manifolds at the heart of the BioSentinel BioSensor, have spurred the development of several additional projects. Most directly is the modified BioSensor that will be a part of LEIA, which will perform its Lunar biology experiment onboard a Commercial Lunar Payload Services lander. Several search-for-life manifolds have been designed to prepare samples from icy moons for downstream analyses. Two early career Polaris projects are developing fluidics to perform genetic sequencing on samples from multigenerational cell culture and to extract and quantify target miRNAs to support astronaut radiation health assessment. Improving the readiness of these systems has been accelerated by adopting the established flight heritage and microgravity-compatibility of the Bio-CubeSat fluidic hardware and designs, while focusing development efforts on the integration of novel functionalities and components.

microfluidics↗
NASA NTRSDate not supplied

The Lunar Explorer Instrument for Space Biology Applications (LEIA) Pre-Flight Tests: Mechanical Considerations​

The BioSensor payload on the upcoming LEIA platform aboard the CLPS (CP-22) lander will carry yeast to the Moon's south pole to study the effects of lunar radiation and gravity. From Earth, BioSensor will transport desiccated yeast within 16 fluidic cards, each containing 16 fluidic wells of 100 microliters volume. At the start of the experiment, these microbes will be autonomously rehydrated on the Moon, and optical absorbance measurements at three wavelengths will be performed across all 256 wells. Absorbance measurements in fluidic wells are susceptible to noise and artifacts due to bubble formation during well rehydration and microbial growth. BioSensor, as a biofluidics system, faces significant data anomalies caused by payload vibrations, particularly from the operation of the co-located PROSPECT’s ProSEED drill, to assess lunar resources by penetrating the subsurface. To evaluate the impact of drill vibrations, fully assembled and filled fluidic cards were subjected to sine sweep signals in the x, y, z planes, ranging from 5 - 2560 Hz at g-loads of 0.25 and 0.5, with on-board accelerometer for localized vibration measurement. Wells with various fill conditions, including partial fills with different bubble sizes and completely filled wells, were tested. Results indicated that small bubbles adhered to well walls at high frequencies but were dislodged at low frequencies, causing data anomalies. Medium and large bubbles exhibited higher interference with optical data due to lower surface tension and variable bubble surface flexion. Overall, it was found that irrespective of the fill type, low-frequency vibrations were most detrimental to optical readings. Understanding the drill schedule and correlating it with BioSensor timestamps will help identify periods when BioSensor data is unreliable, a crucial step for accurately interpreting the microbial response data and ensuring the validity of the BioSensor's scientific measurements on the lunar surface.

Chinmayee Govinda Raj↗
NASA NTRS1972

Feasibility study of a bidirectional jet flap device for application to helicopter rotor blades. Phase 2: Lift controller development

A bidirectional jet flap device called the variable deflection thruster (VDT) has been investigated for possible application to helicopter rotors. This investigation included the development and testing of a fluidic lift control system for the VDT-blade model making use of the test result that VDT-blade lift can be sensed from the differential pressure at midchord. This study constitutes a long-range program to develop blown control techniques for stabilizing the higher harmonic modes of helicopter rotors. Wind tunnel tests were conducted using a three-sectioned, two-dimensional VDT-blade model having individually controlled VDT jet flaps in each section. Steady-state tests were conducted without the fluidic lift controller (open loop) for both full-span blowing and for the model center section blowing only. Steady-state tests were conducted with the center section blowing only using the fluidic lift controller (close-loop) to control the lift on the model center section. Dynamic tests were conducted using the complete model with the VDT jet in the model center section oscillating at various frequencies and also using the model center section alone on a single endplate to obtain finite-aspect-ratio effects. Fair agreement was obtained between theory and experimental results.

Rose, R. E.↗
NASA NTRS2004

Fabrication of Channels for Nanobiotechnological Devices

A method, now undergoing development, of forming nanochannels in planar substrates is intended to enable the fabrication of advanced fluidic devices that could be integrated with complementary metal oxide semiconductor (CMOS) electronic circuits. Such integral combinations of fluidic and electronic components ("laboratory-on-a-chip" devices) could be used, for example, to detect individual molecules of deoxyribonucleic acid (DNA) and proteins. The width of a channel in such a device would be chosen so that molecules of the species of interest would move along the channel in single file. In this method, the fabrication of channels includes the use of such CMOS-compatible processes as chemical-mechanical polishing and oxide deposition. The layout of the channels in the substrate plane is determined by a single photolithographic process, but it is not a nanoscale lithographic process, and this process is not relied upon to define the thickness and width of the channels. Stating it from a slightly different perspective, unlike in the prior fabrication of electronic and fluidic devices involving the use of lithography to define microscale features, this process does not include the use of lithography to define nanoscale features. It is this aspect of the method that enables simplification of the process and, hence, a decrease in cost.

Lee, Choonsup↗
NASA NTRS2014

Helicopter Fuselage Active Flow Control in the Presence of a Rotor

This work extends previous investigations of active flow control for helicopter fuselage drag and download reduction to include the effects of the rotor. The development of the new wind tunnel model equipped with fluidic oscillators is explained in terms of the previous test results. Large drag reductions greater than 20% in some cases were measured during powered testing without increasing, and in some cases decreasing download in forward flight. As confirmed by Particle Image Velocimetry (PIV), the optimum actuator configuration that provided a decrease in both drag and download appeared to create a virtual (fluidic) boat-tail fairing instead of attaching flow to the ramp surface. This idea of a fluidic fairing shifts the focus of 3D separation control behind bluff bodies from controlling/reattaching surface boundary layers to interacting with the wake flow.

Martin, Preston B↗
NASA NTRS2021

BioSensor Users' Guide

The BioSensor is a fully autonomous 3-color LED-based spectrophotometer paired with a fluidics system that supports microbes in liquid culture. Originally developed for the BioSentinel CubeSat mission to study the response of a wild type and mutant strain of yeast to the deep space environment, the BioSensor consists of a series of 16-well, independently plumbed fluidics cards. The cards utilize filters to allow the fluidic system to administer reagents while constraining the microbes in their wells. Heaters on each card incubate to the appropriate growth temperature once the experiment begins. During the active experiment, the LED/detector system measures the transmission of light through each well at three specific wavelengths, similar to a standard benchtop spectrophotometer. The transmission/absorbance kinetics curves for each well are telemetered back to Earth, along with temperature data, for analysis on the ground. The BioSensor is being upgraded from its original CubeSat free-flyer interface to be a secondary payload on lunar landers, Gateway, and other BLEO applications, while maintaining the same functionality and science utility for future experiments.

Matthew Lera↗