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At least 163 records · Page 9

Optimizing inference of segmentation on high-resolution images in MLExchange

MLExchange is a machine learning (ML) operations platform providing web user-interfaces (UIs) for data visualization and analysis pipelines at synchrotron facilities. Among these UIs is the segmentation app which helps synchrotron users utilize ML algorithms to automatically segment high-resolution scientific images with minimal manual annotation effort. In this work, we share code optimizations that significantly speed up the segmentation inference workflow of large data in short time. By optimizing the sequence of CPU-GPU data transfers and introducing CPU parallelization to key operations, we improve the per-device, per-image frame computational efficiency and observe close to 3×$$\times$$ speedup over the original segmentation inference workflow run time when utilizing a single GPU. Further adaptations enabling multi-GPU inference yield more than 40×$$\times$$ speedup with 100 GPUs compared to the optimized single GPU inference workflow. This acceleration of the segmentation inference workflow will provide MLExchange users with easy access to segmentation results with little wait time.

Lu, Shizhao↗

Electron-optic limitations on image resolution

Various approaches are considered to the solution of the electron-optical problem of designing an image tube configuration. Emphasis is placed on the method of computer design, and an illustration is given in which the technique is used in the design of an 80-mm image tube with a zoom capability of 3:1. The solutions are discussed to such problems as image distortion, magnification, and electron bundles striking the zoom electrode. Three types of an electron-optical configuration are examined for the electron-optic limitations to resolution: (1) the proximity image tube, (2) the magnetic-type image tube having uniform electric and magnetic fields, and (3) the electrostatic-type image tube such as the 80-mm zoom tube.

Engstrom, R. W.↗

mpfit: a robust method for fitting atomic resolution images with multiple Gaussian peaks

The standard technique for sub-pixel estimation of atom positions from atomic resolution scanning transmission electron microscopy images relies on fitting intensity maxima or minima with a two-dimensional Gaussian function. While this is a widespread method of measurement, it can be error prone in images with non-zero aberrations, strong intensity differences between adjacent atoms or in situations where the neighboring atom positions approach the resolution limit of the microscope. Here we demonstrate mpfit, an atom finding algorithm that iteratively calculates a series of overlapping two-dimensional Gaussian functions to fit the experimental dataset and then subsequently uses a subset of the calculated Gaussian functions to perform sub-pixel refinement of atom positions. Based on both simulated and experimental datasets presented in this work, this approach gives lower errors when compared to the commonly used single Gaussian peak fitting approach and demonstrates increased robustness over a wider range of experimental conditions.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

High-resolution imaging of globular cluster cores

An approach based on the maximum entropy method aimed at increasing angular resolution to study globular cluster cores is presented. To perform the image restoration the Gull-Skilling (1989) MEMSYS-3 code for maximum entropy reconstruction of arbitrary data sets was used. This software was recently applied to restoration of ESO images of the R136 object in the core of the 30 Doradus nebula. It was demonstrated that the software made it possible to restore an image at subpixel spatial scales which facilitates the detection of very high-resolution structure in the restored image.

Weir, N.↗

High resolution images of P/Tempel 1 and P/Tempel 2 constructed from IRAS survey data

Infrared images of P/Tempel 1 and P/Tempel 2 were constructed from IRAS survey data using a computer algorithm based on the Maximum Correlation Method for Image Construction (Aumann et al, 1990). The resulting images are of sufficiently high quality and resolution to delineate coma and tail morphology, and permit accurate photometry of the total dust complex. Comparisons of the infrared colors and photometric profiles of Tempel 1 and Tempel 2 at similar heliocentric distances show that the grains produced by the two comets are quite similar in radiometric and dynamic properties. Tempel 1 is found to produce about 30 percent more dust in its coma and tail than Tempel 2. The comae of Tempel 1 and Tempel 2 are expanding with mean velocities of 5.8 plus or minus 0.07 and 6.1 plus or minus 0.17 m/sec respectively, indicative of the ejection of large grains. The IRAS cataloged infrared fluxes (Walker, 1986) are found to be underestimated by as much as a factor of three for the comets. Therefore, it is essential to create images of the comets to obtain meaningful IRAS photometry.

Walker, Russell G.↗

High Spatial Resolution Imaging of Mouse Pancreatic Islets Using Nanospray Desorption Electrospray Ionization Mass Spectrometry

Nanospray Desorption Electrospray Ionization mass spectrometry imaging (nano-DESI MSI) enables ambient imaging of biological samples with high sensitivity and minimal sample pretreatment. Recently, we developed an approach for constant-distance mode MSI using shear force microscopy to precisely control the distance between the sample and the nano-DESI probe. Herein, we demonstrate the power of this approach for robust imaging of pancreatic islets with high spatial resolution of ~11 µm. Pancreatic islets are difficult to characterize using traditional mass spectrometry approaches due to their small size (~100 µm) and molecular heterogeneity. Nano-DESI MSI was used to examine the spatial localization of several lipid classes including phosphatidylcholine (PC), phosphatidylethanolamine (PE), sphingomyelin (SM), phosphatidylinositol (PI), and phosphatidylserine (PS) along with fatty acids and their metabolites (e.g., prostaglandins) in the individual islets and surrounding tissue. Several lipids were found to be substantially enhanced in the islets indicating these lipids may be involved in insulin secretion. Remarkably different distributions were observed for several pairs of Lyso PC (LPC) and PC species differing only by one double bond, such as LPC 18:1 vs LPC 18:0, PC 32:1 vs PC 32:0, and PC 34:2 vs PC 34:1. These findings indicate that minor variations in the fatty acid chain length and saturation have a pronounced effect on the localization of PC and LPC species in pancreatic islets. Interestingly, oxidized PC species observed experimentally were found to be specifically localized to pancreatic islets. These PCs are potential biomarkers for reactive oxygen species in the islets, which could be harmful to pancreatic beta cells. The experimental approach presented in this study will provide valuable information on the heterogeneity of individual pancreatic islets, which is difficult to assess using bulk characterization techniques.

Yin, Ruichuan↗

Plant density and enhanced plant vigor estimation for high-resolution images v1

The package contains algorithms for the estimation of sprout/plant density and the corresponding enhanced plant vigor from remotely sensed (RS) images. These algorithms aim to improve the plant characterization and monitoring in the early season when the soil component is significant compared to the plant spatial coverage. In such a scenario, the identification of sprouts (or plants) and the estimation of plant vegetation indexes can be performed only using UAV-acquired images. Additionally, because of the extremely high resolution, gridding becomes necessary to improve visualization and provide more informative maps. However, to capture the real range of values of the VIs, it is important to avoid the inclusion of those pixels belonging to the soil component. The sprout/plant density estimation algorithm takes in input a classification map and uses the vegetation class to estimates the plant density within a user-defined grid using a floating window. Similarly, the enhanced plant vigor algorithm takes in input the vegetation index and the classification map. The enhanced vegetation index is computed for each pixel as the average value among those pixels that belong to the vegetation class within a floating window. The software is developed for the MATLAB environment.

Falco, Nicola↗

MRO High Resolution Imaging Science Experiment (HiRISE): Instrument Development

The primary functional requirement of the HiRISE imager is to allow identification of both predicted and unknown features on the surface of Mars to a much finer resolution and contrast than previously possible. This results in a camera with a very wide swath width, 6km at 300km altitude, and a high signal to noise ratio, >100:1. Generation of terrain maps, 30 cm vertical resolution, from stereo images requires very accurate geometric calibration. The project limitations of mass, cost and schedule make the development challenging. In addition, the spacecraft stability must not be a major limitation to image quality. The nominal orbit for the science phase of the mission is a 3pm orbit of 255 by 320 km with periapsis locked to the south pole. The track velocity is approximately 3,400 m/s.

Alan Delamere↗

Near-IR High-Resolution Imaging Polarimetry of the SU Aur Disk: Clues for Tidal Tails?

We present new high-resolution (approximately 0.09) H-band imaging observations of the circumstellar disk around the T Tauri star SU Aur. Our observations with Subaru-HiCIAO have revealed the presence of scattered light as close as 0.15 (approximately 20 AU) to the star. Within our image, we identify bright emission associated with a disk with a minimum radius of approximately 90 AU, an inclination of approximately 35 deg from the plane of the sky, and an approximate PA of 15 deg for the major axis. We find a brightness asymmetry between the northern and southern sides of the disk due to a non-axisymmetric disk structure. We also identify a pair of asymmetric tail structures extending east and west from the disk. The western tail extends at least 2. 5 (350 AU) from the star, and is probably associated with a reflection nebula previously observed at optical and near-IR wavelengths. The eastern tail extends at least 1 (140 AU) at the present signal-to-noise. These tails are likely due to an encounter with an unseen brown dwarf, but our results do not exclude the explanation that these tails are outflow cavities or jets.

protoplanetary disks↗

Neutron Scattering Instrumentation Research and Development for High Spatial and Temporal Resolution Imaging at Oak Ridge National Laboratory

Neutron scattering facilities like those available at ORNL are enabling the discovery and development of advanced materials include those to be used for next generation energy storage systems. In spite of all the success that neutron imaging has enjoyed in recent years, even higher-impact research is limited by the current temporal and spatial resolution of neutron detection devices. In particular, the spatial resolution of all neutron imaging methods in use and under development is fundamentally limited by the variance introduced by the charged particles emitted from neutron absorption. Thus, we are investigating, designing and demonstrating an array of ~1 micron-sized glass scintillating fibers arranged via photonics-crystal-fiber patterning techniques-- with particle tracking (a must) so that position resolution can be finer than than that allowed by the charged particle ranges. The processes, knowledge and algorithms developed will allow for spatially (1 micron) and time resolved (<100 ns), high throughput neutron transmission imaging in large areas at reasonable costs.

73 NUCLEAR PHYSICS AND RADIATION PHYSICS↗

HIGH-RESOLUTION IMAGING OF THE GEGENSCHEIN AND THE GEOMETRIC ALBEDO OF INTERPLANETARY DUST

We performed optical observations of the Gegenschein using a liquid-nitrogen-cooled wide-field camera, the Wide-field Imager of Zodiacal light with ARray Detector (WIZARD), between 2003 March and 2006 November. We found a narrow brightness enhancement superimposed on the smooth gradient of the Gegenschein at the exact position of the antisolar point. Whereas the Gegenschein morphology changed according to the orbital motion of the Earth, the maximum brightness coincided with the antisolar direction throughout the year. We compared the observed morphology of the Gegenschein with those of models in which the spatial density of the interplanetary dust cloud was considered and found that the volume scattering phase function had a narrow backscattering enhancement. The morphology was reproducible with a spatial distribution model for infrared zodiacal emission. It is likely that the zero-phase peak (the so-called opposition effect) was caused by coherent backscattering and/or shadow-hiding effects on the rough surfaces of individual dust particles. These results suggest that big particles are responsible for both zodiacal light and zodiacal emission. Finally, we derived the geometric albedo of the smooth component of interplanetary dust, assuming big particles, and obtained a geometric albedo of 0.06 {+-} 0.01. The derived albedo is in accordance with collected dark micrometeorites and observed cometary dust particles. We concluded that chondritic particles are dominant near Earth space, supporting the recent theoretical study by dynamical simulation.

79 ASTRONOMY AND ASTROPHYSICS↗

X-ray optics - A technique for high resolution imaging

Both scattering and figure errors in grazing incidence optics are larger in the plane of incidence than out-of-plane by a factor equal to 1/sin theta, where theta is the graze angle. When the full annular aperture of a grazing incidence telescope is stopped down, the point spread function becomes highly elliptical with a width as much as sin theta times narrower than the full image. In practice, this means that improvements in resolution of up to 100 times can be achieved, and effective resolution can approach the diffraction limit. Laboratory data demonstrating the effect are presented.

Cash, Webster↗

Optical system design alternatives for the Moderate-Resolution Imaging Spectrometer-Tilt (MODIS-T) for the Earth Observing System (EOS)

The MODIS consists of two synergistic sensors with a total of 104 spectral bands in the 0.4-14.2-micron range. MODIS-T (Tilt) is one of these sensors. It is a 64-channel imaging spectrometer with a required 10 nm spectral resolution (FWHM) in the 400 to 1040 nm spectral range. The system will have a 1 km IFOV, a wide scan for global coverage in three days, and be capable of being pointed fore and aft of nadir by + or - 50 degrees.

Maymon, Peter W.↗

Real-time atomic-resolution imaging of crystal growth process in water by phase modulation atomic force microscopy at one frame per second

Recent advancement in dynamic-mode atomic force microscopy (AFM) has enabled its operation in liquid with atomic-scale resolution. However, its imaging speed has often been too slow to visualize atomic-scale dynamic processes. Here, we propose a method for making a significant improvement in the operation speed of dynamic-mode AFM. In this method, we use a wideband and low-latency phase detector with an improved algorithm for the signal complexification. We demonstrate atomic-scale imaging of a calcite crystal growth process in water at one frame per second. The significant improvement in the imaging speed should enable various studies on unexplored atomic-scale interfacial processes.

75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND↗

Two-photon interferometry for high-resolution imaging

We discuss advantages of using non-classical states of light for two aspects of optical imaging: creating of miniature images on photosensitive substrates, which constitutes the foundation for optical lithography, and conversely, imaging of micro objects. In both cases, the classical resolution limit given by the Rayleigh criterion is approximately a half of the optical wavelength. It has been shown, however, that by using multi-photon quantum states of the light field, and multi-photon sensitive material or detector, this limit can be surpassed. In the present work, we give a rigorous quantum mechanical treatment of this problem, address some particularly widespread misconceptions and discuss the requirements arising on the way of turning the research on quantum imaging into a practical technology.

quantum technology↗

Localization of Nonblinking Point Sources Using Higher-Order-Mode Detection and Optical Heterodyning: Developing a Strategy for Extending the Scope of Molecular, Super-resolution Imaging

While the stochastic, “blinking” nature of fluorescent systems has enabled the super-resolution of their localization by the fitting of their point-spread functions (PSFs), this strategy cannot be exploited for similar resolution of “nonblinking” systems, such as those that might be encountered in a coherent Raman experiment. An alternative method for subdiffraction-limited imaging lies in the exploitation of optical heterodyning. For example, if a Gaussian PSF (a TEM00 mode) of a point emitter is displaced with respect to the origin of the optical system, photons in the higher-order TEM modes carry information about that displacement. Information concerning the displacement can be extracted from photons in these higher-order modes. These photons can be collected by optical heterodyning, which exploits the large gain in a detector’s response to an optical signal from an emitter coupled to a local oscillator, which is prepared in the TEM of interest, e.g., TEM10. We have generalized and developed the heterodyning technique to localize point emitters via the detection of higher-order spatial modes. We have developed a theoretical approach to find a practical estimation limit of the localization parameters using a realistic model that accounts for shot noise, background noise, and Gaussian noise. To demonstrate the applicability of the method, we designed experiments in which a laser is a surrogate for one and two point emitters. Using the Fisher information and its accompanying Cramér-Rao lower bound, we demonstrate super-resolution localization in these cases: we show that objects can be localized to roughly 2–3 orders of magnitude of their point-spread function’s size for a given optical system. Finally and most importantly, it is suggested that the results will ultimately be generalizable to multiple emitters and, most importantly, to “nonblinking” molecular systems, which will be essential for broadening the scope of super-resolution measurements beyond the limits of fluorescence-based techniques.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

High resolution imaging of very low mass spectral binaries: Three resolved systems and detection of orbital motion in an L/T transition binary

We present high resolution Laser Guide Star Adaptive Optics imaging of 43 late-M, L and T dwarf systems with Keck/NIRC2. These include 17 spectral binary candidates, systems whose spectra suggest the presence of a T dwarf secondary. We resolve three systems: 2MASS J1341–3052, SDSS J1511+0607 and SDSS J2052–1609; the first two are resolved for the first time. All three have projected separations <8 AU and estimated periods of 14–80 years. We also report a preliminary orbit determination for SDSS J2052–1609 based on six epochs of resolved astrometry between 2005 and 2010. Among the 14 unresolved spectral binaries, 5 systems were confirmed binaries but remained unresolved, implying a minimum binary fraction of 47{sub −11}{sup +12}% for this sample. Our inability to resolve most of the spectral binaries, including the confirmed binaries, supports the hypothesis that a large fraction of very low mass systems have relatively small separations and are missed with direct imaging.

79 ASTRONOMY AND ASTROPHYSICS↗

Wide area, coarse resolution imaging with satellite-borne synthetic aperture radars in low-earth and geosynchronous orbits

The LEOSAR (low-earth-orbit synthetic aperture radar) can map around the earth, while the GEOSAR (geosynchronous synthetic aperture radar) can map a large global area bounded in both longitudinal and latitudinal ranges. This paper presents the mapping capabilities and power requirements of both LEOSAR and GEOSAR. For a low-earth-orbit SAR, images of swath widths of the order of 700 km are possible with 100-m resolution and 300 watts of average transmitter power at 9375 MHz. From a SAR in a 50-deg inclined geosynchronous circular orbit, the contiguous United States can be imaged in about 6.4 hours with 100-m resolution, 345 watts of average transmitter power, and a data rate of 6 megabits/sec at 2450 MHz.

Tomiyasu, K.↗