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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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NASA NTRS1994

The Hubble Space Telescope Extragalactic Distance Scale Key Project. 1: The discovery of Cepheids and a new distance to M81

We report on the discovery of 30 new Cepheids in the nearby galaxy M81 based on observations using the Hubble Space Telescope (HST). The periods of these Cepheids lie in the range of 10-55 days, based on 18 independent epochs using the HST wide-band F555W filter. The HST F555W and F785LP data have been transformed to the Cousins standard V and I magnitude system using a ground-based calibration. Apparent period-luminosity relations at V and I were constructed, from which apparent distance moduli were measured with respect to assumed values of mu(sub 0) = 18.50 mag and E(B - V) = 0.10 mag for the Large Magellanic Cloud. The difference in the apparent V and I moduli yields a measure of the difference in the total mean extinction between the M81 and the LMC Cepheid samples. A low total mean extinction to the M81 sample of E(B - V) = 0.03 +/- 0.05 mag is obtained. The true distance modulus to M81 is determined to be 27.80 +/- 0.20 mag, corresponding to a distance of 3.63 +/- 0.34 Mpc. These data illustrate that with an optimal (power-law) sampling strategy, the HST provides a powerful tool for the discovery of extragalactic Cepheids and their application to the distance scale. M81 is the first calibrating galaxy in the target sample of the HST Key Project on the Extragalactic Distance Scale, the ultimate aim of which is to provide a value of the Hubble constant to 10% accuracy.

Freedman, Wendy L.↗
NASA NTRS1993

Resolved stars in nearby galaxies: Ground-based photometry of M81

Using the Canada-France-Hawaii Telescope (CFHT) we have obtained three closely spaced epochs of calibrated Blue Violet Red Infrared (BVRI) CCD imaging of two fields in M81, each known to contain a thirty-day Cepheid. Calibrated BVRI photometry of the brightest stars in these fields is presented. The slope of the luminosity function from the brightest 3-4 mag of the main-sequence blue plume is consistent with similar determinations of the apparent luminosity function in other resolved galaxies, thereby removing the one potential deviation from universality noted by Freedman in a photographic study of luminosity functions in nearby resolved galaxies. Under the assumption that the two Cepheids are representative, a reddening-law fit to the multiwavelength BVRI period-luminosity moduli give a true distance modulus of (m-M)sub 0 = 27.79 mag for M81, corresponding to a linear distance of 3.6 Mpc. An error analysis shows that the derived true distance modulus has a random error of +/- 0.28 mag (due to the photometric uncertainties in the BVRI data), with a systematic uncertainty of +/- 0.10 mag (accounting for the combined effects of unknown phasing of the data points, and the unknown positioning of these particular stars within the Cepheid instabiliy strip).

Madore, Barry F.↗
NASA NTRS1995

Imaging the host galaxies of high-redshift radio-quiet QSOs

We present new deep K-band and optical images of four radio-quiet QSOs at z approximately = 1 and six radio-quiet QSOs at z approximately = 2.5, as well as optical images only of six more at z approximately = 2.5. We have examined the images carefully for evidence of extended 'fuzz' from any putative QSO host galaxy. None of the z approximately = 2.5 QSOs shows any extended emission, and only two of the z approximately = 1 QSOs show marginal evidence for extended emission. Our 3 sigma detection limits in the K images, m(sub K) approximately = 21 for an isolated source, would correspond approximately to an unevolved L(sup star) elliptical galaxy at z = 2.5 or 2-3 mag fainter than an L(sup star) elliptical at z = 1, although our limits on host galaxy light are weaker than this due to the difficulty of separating galaxy light from QSO light. We simulate simple models of disk and elliptical host galaxies, and find that the marginal emission around the two z approximately = 1 QSOs can be explained by disks or bulges that are approximately 1-2 mag brighter than an unevolved L(sup star) galaxy in one case and approximately 1.5-2.5 mag brighter than L(sub star) in the other. For two other z approximately = 1 QSOs, we have only upper limits (L approximately = L(sup star)). The hosts of the high-redshift sample must be no brighter than about 3 mag above an unevolved L(sup star) galaxy, and are at least 1 magnitude fainter than the hosts of radio-loud QSOs at the same redshift. If the easily detected K-band light surrounding a previous sample of otherwise similar but radio-loud QSOs is starlight, then it must evolve on timescales of greater than or approximately equal to 10(exp 8) yr (e.g., Chambers & Charlot 1990); therefore our non-detection of host galaxy fuzz around radio-quiet QSOs supports the view that high-redshift radio-quiet and radio-loud QSOs inhabit different host objects, rather than being single types of objects that turn their radio emission on and off over short timescales. This is consistent with the general trend at low redshifts that radio-loud QSOs are found in giant elliptical galaxies while radio-quiet QSOs are found in less luminous disk galaxies. It also suggests that the processes responsible for the spectacular properties of radio-loud AGNs at high redshifts might not be generally relevent to the (far more numerous) radio-quiet population.

Lowenthal, James D.↗
NASA NTRS1995

Stellar photometry in the inner bulge of M31 using the Hubble Space Telescope wide field camera

We present photometry of two fields in the M31 bulge imaged with the Hubble Space Telescope (HST) Wide-Field Camara (WFC). The nuclear field (r less than 40 arcsecs = 150 pc) giant branch extends to I = 19.5, M(sub I) = -5 (Cousins system), a full 0.9 mag brighter than the giant-branch tips of metal-poor Galactic globular clusters and M31 halo fields. This is also approximately = 1.5 mag brighter than the giant branches of metal-rich Galactic globular clusters, but is no brighter than Mould's (1986) M31 bulge field 1 kpc from the nucleus. The data also suggest that the brighter stars may be preferentially concentrated to the center. The 648 luminous stars detected in 2 x 10(exp 9) solar luminosity is approximately = 25% that expected from a hypothetical population of evolved asymptotic giant branch (AGB) stars with lifetimes approximately = 10(exp 5) yr, with the cautionary note that we are near the detection limit. The number of bright stars is also consistent with the progeny of blue stragglers, if one uses a lifetime for the thermal-pulsing AGB of 2 x 10(exp 6) yr. We strongly caution that incompleteness becomes severe below I = 19.9 mag and that future surveys are likely to find numbers of bright stars too large to accomodate the blue straggler progeny hypothesis. We have imaged an additional field 2 arcmin = 500 pc south of the nucleus. The brightest stars in this field are also I = 19.5, but bright stars appear less numerous than in the nuclear field. If the population resembles that of the Galactic bulge, then M(sub bol) = -4.5 is a lower limit to the giant-branch tip luminosity; infrared studies should reveal stars 0.5 mag or more brighter. Either high-metallicity or (more likely) age approximately = 10 Gyr may be responsible for the presence of these luminous AGB stars. These observations confirm that previous ground-based infrared studies (e.g., Rich & Mould 1991) very likely detect an extended giant branch and not spurious luminous stars caused by crowding or disk contamination. However, published integrated colors for the M31 bulge/nucleus are extremely red, making it difficult to accomodate a young or intermediate-age population.

Rich, R. M.↗
NASA NTRS2011

The SEDs and Host Galaxies of the Dustiest GRB Afterglows

The afterglows and host galaxies of long gamma-ray bursts (GRBs) offer unique opportunities to study star-forming galaxies in the high-z Universe, Until recently, however. the information inferred from GRB follow-up observations was mostly limited to optically bright afterglows. biasing all demographic studies against sight-lines that contain large amounts of dust. Aims. Here we present afterglow and host observations for a sample of bursts that are exemplary of previously missed ones because of high visual extinction (A(sub v) (Sup GRB) approx > 1 mag) along the sight-line. This facilitates an investigation of the properties, geometry and location of the absorbing dust of these poorly-explored host galaxies. and a comparison to hosts from optically-selected samples. Methods. This work is based on GROND optical/NIR and Swift/XRT X-ray observations of the afterglows, and multi-color imaging for eight GRB hosts. The afterglow and galaxy spectral energy distributions yield detailed insight into physical properties such as the dust and metal content along the GRB sight-line as well as galaxy-integrated characteristics like the host's stellar mass, luminosity. color-excess and star-formation rate. Results. For the eight afterglows considered in this study we report for the first time the redshift of GRBs 081109 (z = 0.97S7 +/- 0.0005). and the visual extinction towards GRBs 0801109 (A(sub v) (Sup GRB) = 3.4(sup +0.4) (sub -0.3) mag) and l00621A (A(sub v) (Sup GRB) = 3.8 +/- 0.2 mag), which are among the largest ever derived for GRB afterglows. Combined with non-extinguished GRBs. there is a strong anti-correlation between the afterglow's metals-to-dust ratio and visual extinction. The hosts of the dustiest afterglows are diverse in their properties, but on average redder(((R - K)(sub AB)) approximates 1.6 mag), more luminous ( approximates 0.9 L (sup *)) and massive ((log M(sup *) [M(solar]) approximates 9.8) than the hosts of optically-bright events. We hence probe a different galaxy population. suggesting that previous host samples miss most of the massive. chemically-evolved and metal-rich members. This also indicates that the dust along the sight-line is often related to host properties, and thus probably located in the diffuse ISM or interstellar clouds and not in the immediate GRB environment. Some of the hosts in our sample. are blue, young or of small stellar mass illustrating that even apparently non-extinguished galaxies possess very dusty sight-lines due to a patchy dust distribution. Conclusions. The afterglows and host galaxies of the dustiest GRBs provide evidence for a complex dust geometry in star-forming galaxies. In addition, they establish a population of luminous. massive and correspondingly chemically-evolved GRB hosts. This suggests that GRBs trace the global star-formation rate better than studies based on optically-selected host samples indicate, and the previously-claimed deficiency of high-mass host galaxies was at least partially a selection effect.

Kruhler, T.↗
NASA NTRS2013

The Galex Time Domain Survey. I. Selection And Classification of Over a Thousand Ultraviolet Variable Sources

We present the selection and classification of over a thousand ultraviolet (UV) variable sources discovered in approximately 40 deg(exp 2) of GALEX Time Domain Survey (TDS) NUV images observed with a cadence of 2 days and a baseline of observations of approximately 3 years. The GALEX TDS fields were designed to be in spatial and temporal coordination with the Pan-STARRS1 Medium Deep Survey, which provides deep optical imaging and simultaneous optical transient detections via image differencing. We characterize the GALEX photometric errors empirically as a function of mean magnitude, and select sources that vary at the 5 sigma level in at least one epoch. We measure the statistical properties of the UV variability, including the structure function on timescales of days and years. We report classifications for the GALEX TDS sample using a combination of optical host colors and morphology, UV light curve characteristics, and matches to archival X-ray, and spectroscopy catalogs. We classify 62% of the sources as active galaxies (358 quasars and 305 active galactic nuclei), and 10% as variable stars (including 37 RR Lyrae, 53 M dwarf flare stars, and 2 cataclysmic variables). We detect a large-amplitude tail in the UV variability distribution for M-dwarf flare stars and RR Lyrae, reaching up to absolute value(Δm) = 4.6 mag and 2.9 mag, respectively. The mean amplitude of the structure function for quasars on year timescales is five times larger than observed at optical wavelengths. The remaining unclassified sources include UV-bright extragalactic transients, two of which have been spectroscopically confirmed to be a young core-collapse supernova and a flare from the tidal disruption of a star by dormant supermassive black hole. We calculate a surface density for variable sources in the UV with NUV less than 23 mag and absolute value(Δm) greater than 0.2 mag of approximately 8.0, 7.7, and 1.8 deg(exp −2) for quasars, active galactic nuclei, and RR Lyrae stars, respectively. We also calculate a surface density rate in the UV for transient sources, using the effective survey time at the cadence appropriate to each class, of approximately 15 and 52 deg(exp −2 yr−1 for M dwarfs and extragalactic transients, respectively.

Gezari, S.↗
NASA NTRS2016

Spitzer Space Telescope Mid-IR Light Curves of Neptune

We have used the Spitzer Space Telescope in 2016 February to obtain high cadence, high signal-to-noise, 17 hr duration light curves of Neptune at 3.6 and 4.5 microns. The light curve duration was chosen to correspond to the rotation period of Neptune. Both light curves are slowly varying with time, with full amplitudes of 1.1 mag at 3.6 microns and 0.6 mag at 4.5 microns. We have also extracted sparsely sampled 18 hr light curves of Neptune at W1 (3.4 microns) and W2 (4.6 microns) from the Wide-field Infrared Survey Explorer (WISE)/NEOWISE archive at six epochs in 2010-2015. These light curves all show similar shapes and amplitudes compared to the Spitzer light curves but with considerable variation from epoch to epoch. These amplitudes are much larger than those observed with Kepler/K2 in the visible (amplitude approximately 0.02 mag) or at 845 nm with the Hubble Space Telescope (HST) in 2015 and at 763 nm in 2016 (amplitude approximately 0.2 mag). We interpret the Spitzer and WISE light curves as arising entirely from reflected solar photons, from higher levels in Neptune's atmosphere than for K2. Methane gas is the dominant opacity source in Neptune's atmosphere, and methane absorption bands are present in the HST 763 and 845 nm, WISE W1, and Spitzer 3.6 micron filters.

Individual (Neptune)↗
NASA NTRS2023

Long-term 4.6 µm Variability in Brown Dwarfs and a New Technique for Identifying Brown Dwarf Binary Candidates

Using a sample of 361 nearby brown dwarfs, we have searched for 4.6 μm variability, indicative of large-scale rotational modulations or large-scale, long-term changes on timescales of over 10 yr. Our findings show no statistically significant variability in Spitzer's Infrared Array Camera (IRAC) channel 2 (ch2) or Wide-field Infrared Survey Explorer W2 photometry. For Spitzer the ch2 1σ limits are ∼8 mmag for objects at 11.5 mag and ∼22 mmag for objects at 16 mag. This corresponds to no variability above 4.5% at 11.5 mag and 12.5% at 16 mag. We conclude that highly variable brown dwarfs, at least two previously published examples of which have been shown to have 4.6 μm variability above 80 mmag, are very rare. While analyzing the data, we also developed a new technique for identifying brown dwarf binary candidates in Spitzer data. We find that known binaries have IRAC ch2 point response function (PRF) flux measurements that are consistently dimmer than aperture flux measurements. We have identified 59 objects that exhibit such PRF versus aperture flux differences and are thus excellent binary brown dwarf candidates.

Hunter Brooks↗
DOE OSTI2022

Metagenomics-resolved genomics provides novel insights into chitin turnover, metabolic specialization, and niche partitioning in the octocoral microbiome

Abstract Background The role of bacterial symbionts that populate octocorals (Cnidaria, Octocorallia) is still poorly understood. To shed light on their metabolic capacities, we examined 66 high-quality metagenome-assembled genomes (MAGs) spanning 30 prokaryotic species, retrieved from microbial metagenomes of three octocoral species and seawater. Results Symbionts of healthy octocorals were affiliated with the taxa Endozoicomonadaceae , Candidatus Thioglobaceae , Metamycoplasmataceae , unclassified Pseudomonadales , Rhodobacteraceae , unclassified Alphaproteobacteria and Ca. Rhabdochlamydiaceae . Phylogenomics inference revealed that the Endozoicomonadaceae symbionts uncovered here represent two species of a novel genus unique to temperate octocorals, here denoted Ca. Gorgonimonas eunicellae and Ca. Gorgonimonas leptogorgiae . Their genomes revealed metabolic capacities to thrive under suboxic conditions and high gene copy numbers of serine-threonine protein kinases, type 3-secretion system, type-4 pili, and ankyrin-repeat proteins, suggesting excellent capabilities to colonize, aggregate, and persist inside their host. Contrarily, MAGs obtained from seawater frequently lacked symbiosis-related genes. All Endozoicomonadaceae symbionts harbored endo-chitinase and chitin-binging protein-encoding genes, indicating that they can hydrolyze the most abundant polysaccharide in the oceans. Other symbionts, including Metamycoplasmataceae and Ca. Thioglobaceae , may assimilate the smaller chitin oligosaccharides resulting from chitin breakdown and engage in chitin deacetylation, respectively, suggesting possibilities for substrate cross-feeding and a role for the coral microbiome in overall chitin turnover. We also observed sharp differences in secondary metabolite production potential between symbiotic lineages. Specific Proteobacteria taxa may specialize in chemical defense and guard other symbionts, including Endozoicomonadaceae , which lack such capacity. Conclusion This is the first study to recover MAGs from dominant symbionts of octocorals, including those of so-far unculturable Endozoicomonadaceae , Ca. Thioglobaceae and Metamycoplasmataceae symbionts. We identify a thus-far unanticipated, global role for Endozoicomonadaceae symbionts of corals in the processing of chitin, the most abundant natural polysaccharide in the oceans and major component of the natural zoo- and phytoplankton feed of octocorals. We conclude that niche partitioning, metabolic specialization, and adaptation to low oxygen conditions among prokaryotic symbionts likely contribute to the plasticity and adaptability of the octocoral holobiont in changing marine environments. These findings bear implications not only for our understanding of symbiotic relationships in the marine realm but also for the functioning of benthic ecosystems at large.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2025

Metagenome-assembled genomes from soil samples in control and warming plots in Blodgett Forest, CA (2014-2021)

The pathways of carbon transport and loss through and from soils—soil organic matter (SOM) depolymerization to dissolved organic carbon and mineralization to carbon dioxide (CO2)—are fundamentally driven by microbial activity, which is strongly regulated by environmental conditions. As part of LBNL (Lawrence Berkeley National Laboratory) TES (Terrestrial Ecosystem Science) Belowground Biogeochemistry Science Focus Area (SFA), we have established a novel whole-soil long-term warming experiment at the University of California (UC) Blodgett Forest Research Station (Sierra Nevada) in 2014, where we study the role of biogeochemical, microbial and geochemical process interactions in SOM decomposition and stabilization.Here, we present metagenome-assembled genomes (MAGs) for the bacterial and archaeal community from soil depth profiles collected from 2014 to 2021 from three paired control and warming plots. We collected soil samples across a range of depth profiles (spanning surface to 90 cm deep) from three paired control and warming plots from a temperate mixed forest in Northern California. Each paired plot had been subjected to experimental warming since June 2014 to simulate a predicted climate change scenario for northern California. 101 soil metagenomes were sequenced at JGI (Joint Genome Institute) and UCSF (University of California San Francisco) Center for Advanced Technology and can be found under the JGI (Joint Genome Institute) GOLD (Genomes Online Database) Sequencing project Gs0151586 and NCBI (National Center for Biotechnology Information) Projects PRJNA1225762 and PRJEB39497. Metagenomes were assembled using JGI (Joint Genome Institute) Metagenome Workflow (10.1128/mSystems.00804-20). For each metagenome, the assembled contigs were binned into genomes using 3 binning algorithms (cocacola, metabat, and maxbin) and the resulting bins were consolidated using dastool. The consolidated bins from all metagenomes were pooled, filtered by completeness (>50%) and contamination (<25%), and dereplicated at 99% ANI (average nucleotide identity) using dRep (https://github.com/MrOlm/drep).The dataset includes a zip file of 2321 MAG (Metagenome Assembled Genome) fasta files, the accession numbers for the underlying metagenomes, and a csv file with MAG (Metagenome Assembled Genome) quality metrics and taxonomic classification (GTDB -Genome Taxonomy Database-RS220). This dataset also includes a file-level metadata (flmd.csv) file that lists each file contained in the dataset with associated metadata and a data dictionary (dd.csv) file that contains column/row headers used throughout the files along with a definition, units, and data type. A sample metadata file (samples.csv) that contains site information has also been included.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2022

Discovery of the most luminous quasar of the last 9 Gyr

Abstract We report the discovery of a bright ( $g = 14.5$ mag (AB), $K = 11.9$ mag (Vega)) quasar at redshift $z=0.83$ — the optically brightest (unbeamed) quasar at $z>0.4$ . SMSS J114447.77-430859.3, at a Galactic latitude of $b=+18.1^{\circ}$ , was identified by its optical colours from the SkyMapper Southern Survey (SMSS) during a search for symbiotic binary stars. Optical and near-infrared spectroscopy reveals broad Mg ii , H $\unicode{x03B2}$ , H $\unicode{x03B1}$ , and Pa $\unicode{x03B2}$ emission lines, from which we measure a black hole mass of $\log_{10}\! (M_{\mathrm{BH}}/\mathrm{M}_{\odot}) = 9.4 \pm 0.5$ . With its high luminosity, $L_{\mathrm{bol}} = (4.7\pm1.0)\times10^{47}\,\mathrm{erg\,s}^{-1}$ or $M_{i}(z=2) = -29.74$ mag (AB), we estimate an Eddington ratio of $\approx1.4$ . As the most luminous quasar known over the last ${\sim}$ 9 Gyr of cosmic history, having a luminosity $8\times$ greater than 3C 273, the source offers a range of potential follow-up opportunities.

Astronomy & Astrophysics↗
DOE OSTI2021

Diverse ecophysiological adaptations of subsurface Thaumarchaeota in floodplain sediments revealed through genome-resolved metagenomics

Abstract The terrestrial subsurface microbiome contains vastly underexplored phylogenetic diversity and metabolic novelty, with critical implications for global biogeochemical cycling. Among the key microbial inhabitants of subsurface soils and sediments are Thaumarchaeota, an archaeal phylum that encompasses ammonia-oxidizing archaea (AOA) as well as non-ammonia-oxidizing basal lineages. Thaumarchaeal ecology in terrestrial systems has been extensively characterized, particularly in the case of AOA. However, there is little knowledge on the diversity and ecophysiology of Thaumarchaeota in deeper soils, as most lineages, particularly basal groups, remain uncultivated and underexplored. Here we use genome-resolved metagenomics to examine the phylogenetic and metabolic diversity of Thaumarchaeota along a 234 cm depth profile of hydrologically variable riparian floodplain sediments in the Wind River Basin near Riverton, Wyoming. Phylogenomic analysis of the metagenome-assembled genomes (MAGs) indicates a shift in AOA population structure from the dominance of the terrestrial Nitrososphaerales lineage in the well-drained top ~100 cm of the profile to the typically marine Nitrosopumilales in deeper, moister, more energy-limited sediment layers. We also describe two deeply rooting non-AOA MAGs with numerous unexpected metabolic features, including the reductive acetyl-CoA (Wood-Ljungdahl) pathway, tetrathionate respiration, a form III RuBisCO, and the potential for extracellular electron transfer. These MAGs also harbor tungsten-containing aldehyde:ferredoxin oxidoreductase, group 4f [NiFe]-hydrogenases and a canonical heme catalase, typically not found in Thaumarchaeota. Our results suggest that hydrological variables, particularly proximity to the water table, impart a strong control on the ecophysiology of Thaumarchaeota in alluvial sediments.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2021

Brockarchaeota, a novel archaeal phylum with unique and versatile carbon cycling pathways

Geothermal environments, such as hot springs and hydrothermal vents, are hotspots for carbon cycling and contain many poorly described microbial taxa. Here, we reconstructed 15 archaeal metagenome-assembled genomes (MAGs) from terrestrial hot spring sediments in China and deep-sea hydrothermal vent sediments in Guaymas Basin, Gulf of California. Phylogenetic analyses of these MAGs indicate that they form a distinct group within the TACK superphylum, and thus we propose their classification as a new phylum, ‘Brockarchaeota’, named after Thomas Brock for his seminal research in hot springs. Based on the MAG sequence information, we infer that some Brockarchaeota are uniquely capable of mediating non-methanogenic anaerobic methylotrophy, via the tetrahydrofolate methyl branch of the Wood-Ljungdahl pathway and reductive glycine pathway. The hydrothermal vent genotypes appear to be obligate fermenters of plant-derived polysaccharides that rely mostly on substrate-level phosphorylation, as they seem to lack most respiratory complexes. In contrast, hot spring lineages have alternate pathways to increase their ATP yield, including anaerobic methylotrophy of methanol and trimethylamine, and potentially use geothermally derived mercury, arsenic, or hydrogen. Their broad distribution and their apparent anaerobic metabolic versatility indicate that Brockarchaeota may occupy previously overlooked roles in anaerobic carbon cycling.

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2023

Seasonal activities of the phyllosphere microbiome of perennial crops

Understanding the interactions between plants and microorganisms can inform microbiome management to enhance crop productivity and resilience to stress. Here, we apply a genome-centric approach to identify ecologically important leaf microbiome members on replicated plots of field-grown switchgrass and miscanthus, and to quantify their activities over two growing seasons for switchgrass. We use metagenome and metatranscriptome sequencing and curate 40 medium- and high-quality metagenome-assembled-genomes (MAGs). We find that classes represented by these MAGs (Actinomycetia, Alpha- and Gamma- Proteobacteria, and Bacteroidota) are active in the late season, and upregulate transcripts for short-chain dehydrogenase, molybdopterin oxidoreductase, and polyketide cyclase. Stress-associated pathways are expressed for most MAGs, suggesting engagement with the host environment. We also detect seasonally activated biosynthetic pathways for terpenes and various non-ribosomal peptide pathways that are poorly annotated. Our findings support that leaf-associated bacterial populations are seasonally dynamic and responsive to host cues.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2020

Optical follow-up of the neutron star–black hole mergers S200105ae and S200115j

LIGO and Virgo’s third observing run revealed the first neutron star–black hole (NSBH) merger candidates in gravitational waves. These events are predicted to synthesize r-process elements creating optical/near-infrared ‘kilonova’ emission. The joint gravitational wave and electromagnetic detection of an NSBH merger could be used to constrain the equation of state of dense nuclear matter, and independently measure the local expansion rate of the Universe. Here, we present the optical follow-up and analysis of two of the only three high-significance NSBH merger candidates detected to date, S200105ae and S200115j, with the Zwicky Transient Facility. The Zwicky Transient Facility observed ~48% of S200105ae and ~22% of S200115j’s localization probabilities, with observations sensitive to kilonovae brighter than –17.5 mag fading at 0.5 mag d –1 in the g- and r-bands; extensive searches and systematic follow-up of candidates did not yield a viable counterpart. We present state-of-the-art kilonova models tailored to NSBH systems that place constraints on the ejecta properties of these NSBH mergers. Here, we show that with observed depths of apparent magnitude ~22 mag, attainable in metre-class, wide-field-of-view survey instruments, strong constraints on ejecta mass are possible, with the potential to rule out low mass ratios, high black hole spins and large neutron star radii.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2021

Genomic and functional analyses of fungal and bacterial consortia that enable lignocellulose breakdown in goat gut microbiomes

Abstract The herbivore digestive tract is home to a complex community of anaerobic microbes that work together to break down lignocellulose. These microbiota are an untapped resource of strains, pathways and enzymes that could be applied to convert plant waste into sugar substrates for green biotechnology. We carried out more than 400 parallel enrichment experiments from goat faeces to determine how substrate and antibiotic selection influence membership, activity, stability and chemical productivity of herbivore gut communities. We assembled 719 high-quality metagenome-assembled genomes (MAGs) that are unique at the species level. More than 90% of these MAGs are from previously unidentified herbivore gut microorganisms. Microbial consortia dominated by anaerobic fungi outperformed bacterially dominated consortia in terms of both methane production and extent of cellulose degradation, which indicates that fungi have an important role in methane release. Metabolic pathway reconstructions from MAGs of 737 bacteria, archaea and fungi suggest that cross-domain partnerships between fungi and methanogens enabled production of acetate, formate and methane, whereas bacterially dominated consortia mainly produced short-chain fatty acids, including propionate and butyrate. Analyses of carbohydrate-active enzyme domains present in each anaerobic consortium suggest that anaerobic bacteria and fungi employ mostly complementary hydrolytic strategies. The division of labour among herbivore anaerobes to degrade plant biomass could be harnessed for industrial bioprocessing.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2024

Coassembly and binning of a twenty-year metagenomic time-series from Lake Mendota

Abstract The North Temperate Lakes Long-Term Ecological Research (NTL-LTER) program has been extensively used to improve understanding of how aquatic ecosystems respond to environmental stressors, climate fluctuations, and human activities. Here, we report on the metagenomes of samples collected between 2000 and 2019 from Lake Mendota, a freshwater eutrophic lake within the NTL-LTER site. We utilized the distributed metagenome assembler MetaHipMer to coassemble over 10 terabases (Tbp) of data from 471 individual Illumina-sequenced metagenomes. A total of 95,523,664 contigs were assembled and binned to generate 1,894 non-redundant metagenome-assembled genomes (MAGs) with ≥50% completeness and ≤10% contamination. Phylogenomic analysis revealed that the MAGs were nearly exclusively bacterial, dominated by Pseudomonadota (Proteobacteria, N = 623) and Bacteroidota (N = 321). Nine eukaryotic MAGs were identified by eukCC with six assigned to the phylum Chlorophyta. Additionally, 6,350 high-quality viral sequences were identified by geNomad with the majority classified in the phylum Uroviricota. This expansive coassembled metagenomic dataset provides an unprecedented foundation to advance understanding of microbial communities in freshwater ecosystems and explore temporal ecosystem dynamics.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2024

Ultra-deep imaging of NGC 1052-DF2 and NGC 1052-DF4 to unravel their origins

A number of scenarios have been proposed to explain the low velocity dispersion (and hence possible absence of dark matter) of the low surface brightness galaxies NGC 1052-DF2 and NGC 1052-DF4. Most of the proposed mechanisms are based on the removal of dark matter via the interaction of these galaxies with other objects. A common feature of these processes is the prediction of very faint tidal tails, which should be revealed by deep imaging (μ g > 30 mag arcsec −2 ). Using ultra-deep images obtained with the Gemini telescopes, about 1 mag deeper than previously published data, we analyzed the possible presence of tidal tails in both galaxies. We confirm the presence of tidal tails in NGC 1052-DF4, but see no evidence for tidal effects in NGC 1052-DF2, down to surface brightnesses ofμ g = 30.9 mag arcsec −2 . We therefore conclude that while the absence of dark matter in NGC 1052-DF4 could be attributed to the removal of dark matter by gravitational interactions, in the case of NGC 1052-DF2 this explanation seems less plausible, and therefore other possibilities such as an incorrect distance measurement or that the system may be rotating could alleviate the dark matter problem.

Astronomy & Astrophysics↗