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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 163 records · Page 9
All sourcesNASA NTRSDOE OSTI
NASA NTRS1992

To build a mine: Prospect to product

The terrestrial definition of ore is a quantity of earth materials containing a mineral that can be extracted at a profit. While a space-based resource-gathering operation may well be driven by other motives, such an operation should have the most favorable cost-benefit ratio possible. To this end, principles and procedures already tested by the stringent requirements of the profit motive should guide the selection, design, construction, and operation of a space-based mine. Proceeding from project initiation to a fully operational mine requires several interacting and overlapping steps, which are designed to facilitate the decision process and insure economic viability. The steps to achieve a fully operational mine are outlined. Presuming that the approach to developing nonterrestrial resources will parallel that for developing mineral resources on Earth, we can speculate on some of the problems associated with developing lunar and asteroidal resources. The baseline for our study group was a small lunar mine and oxygen extraction facility. The development of this facility is described in accordance with the steps outlined.

Gertsch, Richard E.↗
NASA NTRS1993

The development of hydrogen sensor technology at NASA Lewis Research Center

The detection of hydrogen leaks in aerospace applications, especially those involving hydrogen fuel propulsion systems, is of extreme importance for reasons of reliability, safety, and economy. Motivated by leaks occurring in liquid hydrogen lines supplying the main engine of the Space Shuttle, NASA Lewis has initiated a program to develop point-contact hydrogen sensors which address the needs of aerospace applications. Several different approaches are being explored. They include the fabrication of PdAg Schottky diode structures, the characterization of PdCr as a hydrogen sensitive alloy, and the use of SiC as a semiconductor for hydrogen sensors. This paper discusses the motivation behind and present status of each of the major components of the NASA LeRC hydrogen sensor program.

Hunter, Gary W.↗
NASA NTRS1991

The best-fit universe

Inflation provides very strong motivation for a flat Universe, Harrison-Zel'dovich (constant-curvature) perturbations, and cold dark matter. However, there are a number of cosmological observations that conflict with the predictions of the simplest such model: one with zero cosmological constant. They include the age of the Universe, dynamical determinations of Omega, galaxy-number counts, and the apparent abundance of large-scale structure in the Universe. While the discrepancies are not yet serious enough to rule out the simplest and most well motivated model, the current data point to a best-fit model with the following parameters: Omega(sub B) approximately equal to 0.03, Omega(sub CDM) approximately equal to 0.17, Omega(sub Lambda) approximately equal to 0.8, and H(sub 0) approximately equal to 70 km/(sec x Mpc) which improves significantly the concordance with observations. While there is no good reason to expect such a value for the cosmological constant, there is no physical principle that would rule out such.

Turner, Michael S.↗
NASA NTRS1992

Pulse-Timing Studies of X Ray Pulsars

The pulse-timing projects supported by NASA Grant NAG8-695 were motivated in large part by our long-standing interest in the physics of rotating neutron stars and particularly the variations in rotation due to internal and external fluctuating torques. For accretion-powered pulsars, observed as compact galactic X-ray sources, our work has been motivated by questions regarding the physics of matter accretion, mass transfer, and mass loss in these X-ray binary systems. For rotation-powered pulsars using X-ray, optical, and radio observations, we have examined the internal structure of neutron stars and mechanisms for secular spin-down. These issues are still central to the continuing process of understanding the complex behavior of these fascinating systems. The work supported by this grant is based mainly on Ginga observations of three pulsating X-ray sources. We observed two of these sources (Her X-1 and SMC X-1) within the framework of a NASA-ISAS program for U.S.-Japan collaborations to obtain and analyze Ginga observations. In addition, we joined with members of the Ginga team to apply our pulse-timing methods to PSR 0540-69 data obtained as a collateral benefit of the regular monitoring by Ginga of SN 1987A. In addition to these Ginga observations, there exist other relevant data on all three of these sources. The direction of our investigations has been affected by the necessity of including these supporting data in our analyses, and setting up the necessary collaborations has sometimes entailed extra work that was not anticipated in our proposals. Indeed, we have often taken the initiative in establishing these joint projects.

Deeter, John E.↗
NASA NTRS1994

Space technology: A study of the significance of recognition for innovators of spinoff technologies. 1993 activities/1994, 1995 plans

During the past 30 years as NASA has conducted technology transfer programs, it has gained considerable experience - particularly pertaining to the processes. However, three areas have not had much scrutiny: the examination of the contributions of the individuals who have developed successful spinoffs, the commercial success of the spinoffs themselves, and the degree to which they are understood by the public. In short, there has been limited evaluation to measure the success of technology transfer efforts mandated by Congress. Research conducted during the first year of a three-year NASA grant to the United States Space Foundation has taken the initial steps toward measuring the success of methodologies to accomplish that Congressionally-mandated technology transfer. In particular, the US Space Foundation, in cooperation with ARAC, technology transfer experts; JKA, a nationally recognized themed entertainment design company; and top evaluation consultants, inaugurated and evaluated a fresh approach including commercial practices to encourage, motivate, and energize technology transfer by: recognizing already successful efforts (Space Technology Hall of Fame Award), drawing potential business and industrial players into the process (Space Commerce Expo), and informing and motivating the general public (Space Technology Hall of Fame public venues). The first year's efforts are documented and directions for the future are outlined.

Source record↗
NASA NTRS1993

Implementing abstract multigrid or multilevel methods

Multigrid methods can be formulated as an algorithm for an abstract problem that is independent of the partial differential equation, domain, and discretization method. In such an abstract setting, problems not arising from partial differential equations can be treated. A general theory exists for linear problems. The general theory was motivated by a series of abstract solvers (Madpack). The latest version was motivated by the theory. Madpack now allows for a wide variety of iterative and direct solvers, preconditioners, and interpolation and projection schemes, including user callback ones. It allows for sparse, dense, and stencil matrices. Mildly nonlinear problems can be handled. Also, there is a fast, multigrid Poisson solver (two and three dimensions). The type of solvers and design decisions (including language, data structures, external library support, and callbacks) are discussed. Based on the author's experiences with two versions of Madpack, a better approach is proposed. This is based on a mixed language formulation (C and FORTRAN + preprocessor). Reasons for not using FORTRAN, C, or C++ (individually) are given. Implementing the proposed strategy is not difficult.

Douglas, Craig C.↗
NASA NTRS1993

Containerless measurements on liquids at high temperatures

The application of containerless techniques for measurements of the thermophysical properties of high temperature liquids is reviewed. Recent results obtained in the materials research laboratories at Intersonics are also presented. Work to measure high temperature liquid properties is motivated by both the need for reliable property data for modeling of industrial processes involving molten materials and generation of data form basic modeling of materials behavior. The motivation for this work and examples of variations in thermophysical property values from the literature are presented. The variations may be attributed to changes in the specimen properties caused by chemical changes in the specimen and/or to measurement errors. The two methods used to achieve containerless conditions were aeroacoustic levitation and electromagnetic levitation. Their qualities are presented. The accompanying slides show the layout of levitation equipment and present examples of levitated metallic and ceramic specimens. Containerless techniques provide a high degree of control over specimen chemistry, nucleation and allow precise control of liquid composition to be achieved. Effects of minor additions can thus be measured in a systematic way. Operation in reduced gravity enables enhanced control of liquid motion which can allow measurement of liquid transport properties. Examples of nucleation control, the thermodynamics of oxide contamination removal, and control of the chromium content of liquid aluminum oxide by high temperature containerless processes are presented. The feasibility of measuring temperature, emissivity, liquidus temperature, enthalpy, surface tension, density, viscosity, and thermal diffusivity are discussed in the final section of the paper.

Weber, Richard↗
NASA NTRS1994

The Accuracy of Parameter Estimation in System Identification of Noisy Aircraft Load Measurement

This thesis focuses on the subject of the accuracy of parameter estimation and system identification techniques. Motivated by a complicated load measurement from NASA Dryden Flight Research Center, advanced system identification techniques are needed. The objective of this problem is to accurately predict the load experienced by the aircraft wing structure during flight determined from a set of calibrated load and gage response relationship. We can then model the problem as a black box input-output system identification from which the system parameter has to be estimated. Traditional LS (Least Square) techniques and the issues of noisy data and model accuracy are addressed. A statistical bound reflecting the change in residual is derived in order to understand the effects of the perturbations on the data. Due to the intrinsic nature of the LS problem, LS solution faces the dilemma of the trade off between model accuracy and noise sensitivity. A method of conflicting performance indices is presented, thus allowing us to improve the noise sensitivity while at the same time configuring the degredation of the model accuracy. SVD techniques for data reduction are studied and the equivalence of the Correspondence Analysis (CA) and Total Least Squares Criteria are proved. We also looked at nonlinear LS problems with NASA F-111 data set as an example. Conventional methods are neither easily applicable nor suitable for the specific load problem since the exact model of the system is unknown. Neural Network (NN) does not require prior information on the model of the system. This robustness motivated us to apply the NN techniques on our load problem. Simulation results for the NN methods used in both the single load and the 'warning signal' problems are both useful and encouraging. The performance of the NN (for single load estimate) is better than the LS approach, whereas no conventional approach was tried for the 'warning signals' problems. The NN design methodology is also presented. The use of SVD, CA and Collinearity Index methods are used to reduce the number of neurons in a layer.

Kong, Jeffrey↗
NASA NTRS1995

Design of high performance multivariable control systems for supermaneuverable aircraft at high angle of attack

The main motivation for the work under the present grant was to use nonlinear feedback linearization methods to further enhance performance capabilities of the aircraft, and robustify its response throughout its operating envelope. The idea was to use these methods in lieu of standard Taylor series linearization, in order to obtain a well behaved linearized plant, in its entire operational regime. Thus, feedback linearization was going to constitute an 'inner loop', which would then define a 'design plant model' to be compensated for robustness and guaranteed performance in an 'outer loop' application of modern linear control methods. The motivation for this was twofold; first, earlier work had shown that by appropriately conditioning the plant through conventional, simple feedback in an 'inner loop', the resulting overall compensated plant design enjoyed considerable enhancement of performance robustness in the presence of parametric uncertainty. Second, the nonlinear techniques did not have any proven robustness properties in the presence of unstructured uncertainty; a definition of robustness (and performance) is very difficult to achieve outside the frequency domain; to date, none is available for the purposes of control system design. Thus, by proper design of the outer loop, such properties could still be 'injected' in the overall system.

Valavani, Lena↗
NASA NTRS1993

The Influence of Swirl Brakes and a Tip Discharge Orifice on the Rotordynamic Forces Generated by Discharge-to-Suction Leakage Flows in Shrouded Centrifugal Pumps

Recent experiments conducted in the Rotor Force Test Facility at the California Institute of Technology have examined the effects of a tip leakage restriction and swirl brakes on the rotordynamic forces due to leakage flows on an impeller undergoing a prescribed circular whirl. The experiments simulate the leakage flow conditions and geometry of the Alternate Turbopump Design (ATD) of the Space Shuttle High Pressure Oxygen Turbopump and are critical to evaluating the pump's rotordynamic instability problems. Previous experimental and analytical results have shown that discharge-to-suction leakage flows in the annulus of a shrouded centrifugal pump contribute substantially to the fluid induced rotordynamic forces. Also, previous experiments have shown that leakage inlet (pump discharge) swirl can increase the cross-coupled stiffness coefficient and hence increase the range of positive whirl for which the tangential force is destabilizing. In recent experimental work, the present authors demonstrated that when the swirl velocity within the leakage path is reduced by the introduction of ribs or swirl brakes, then a substantial decrease in both the destabilizing normal and tangential forces could be achieved. Motivation for the present research is that previous experiments have shown that restrictions such as wear rings or orifices at pump inlets affect the leakage forces. Recent pump designs such as the Space Shuttle Alternate Turbopump Design (ATD) utilize tip orifices at discharge for the purpose of establishing axial thrust balance. The ATD has experienced rotordynamic instability problems and one may surmise that these tip discharge orifices may also have an important effect on the normal and tangential forces in the plane of impeller rotation. The present study determines if such tip leakage restrictions contribute to undesirable rotordynamic forces. Additional motivation for the present study is that the widening of the leakage path annular clearance and the installation of swirl brakes in the ATD has been proposed to solve its instability problems. The present study assesses the effect of such a design modification on the rotordynamic forces. The experimental apparatus consists of a solid or dummy impeller, a housing instrumented for pressure measurements, a rotating dynamometer and an eccentric whirl mechanism. The solid impeller is used so that leakage flow contributions to the forces are measured, but the main throughflow contributions are not experienced. The inner surface of the housing has been modified to accommodate meridional ribs or swirl brakes within the leakage annulus. In addition, the housing has been modified to accommodate a discharge orifice that qualitatively simulates one side of the balance piston orifice of the Space Shuttle ATD. Results indicate the detrimental effects of a discharge orifice and the beneficial effects of brakes. Plots of the tangential and normal forces versus whirl ratio show a substantial increase in these forces along with destabilizing resonances at some positive whirl ratios when a discharge orifice is added. When brakes are added, some of the detrimental effects of the orifice are reduced. For the tangential force, a plot versus whirl ratio shows a significant reduction and a destabilizing resonance appears to be eliminated. For the normal force, although the overall force is not reduced, again a destabilizing resonance appears to be eliminated.

Sivo, Joseph M.↗
NASA NTRS1994

Comparative transduction mechanisms of hair cells in the bullfrog uticulus. 2: Sensitivity and response dynamics to hair bundle displacement

The present study was motivated by an interest in seeing whether hair cell types in the bullfrog utriculus might differ in their voltage responses to hair bundle displacement. Particular interest was in assessing the contributions of two factors to the responses of utricular hair cells. First, interest in examining the effect of hair bundle morphology on the sensitivity of hair cells to natural stimulation was motivated by the observation that vestibular hair cells, unlike many auditory hair cells, are not free-standing but rather linked to an accessory cupular or otolithic membrane via the tip of their kinocilium. Interest also laid in examining the contribution, if any, of adaptation to the response properties of utricular hair cells. Hair cells in auditory and vibratory inner ear endorgans adapt to maintained displacements of their hair bundles, sharply limiting their low frequency sensitivity. This adaptation is mediated by a shift in the displacement-response curve (DRC) of the hair cell along the displacement axis. Observations suggest that the adaptation process occurs within the hair bundle and precedes mechanoelectric transduction. Recent observations of time-dependent changes in hair bundle stiffness are consistent with this conclusion. Adaptation would be expected to be most useful in inner ear endorgans in which hair cells are subject to large static displacements that could potentially saturate their instantaneous response and compromise their sensitivity to high frequency stimulation. The adaptation process also permits hair cells to maintain their sensory hair bundle in the most sensitive portion of their DRC. In vestibular otolith organs in which static sensitivity is desirable, any adaptation process in the hair cells may be undesirable. The rate and extent of the decline of the voltage responses was measured of utricular hair cells to step and sinusoidal hair bundle displacements. Then for similar resting potentials and response amplitudes, the voltage responses of individual hair cells were compared to both hair bundle displacement and intracellular current.

Baird, Richard A.↗
NASA NTRS1995

Inductive Approaches to Improving Diagnosis and Design for Diagnosability

The first research area under this grant addresses the problem of classifying time series according to their morphological features in the time domain. A supervised learning system called CALCHAS, which induces a classification procedure for signatures from preclassified examples, was developed. For each of several signature classes, the system infers a model that captures the class's morphological features using Bayesian model induction and the minimum message length approach to assign priors. After induction, a time series (signature) is classified in one of the classes when there is enough evidence to support that decision. Time series with sufficiently novel features, belonging to classes not present in the training set, are recognized as such. A second area of research assumes two sources of information about a system: a model or domain theory that encodes aspects of the system under study and data from actual system operations over time. A model, when it exists, represents strong prior expectations about how a system will perform. Our work with a diagnostic model of the RCS (Reaction Control System) of the Space Shuttle motivated the development of SIG, a system which combines information from a model (or domain theory) and data. As it tracks RCS behavior, the model computes quantitative and qualitative values. Induction is then performed over the data represented by both the 'raw' features and the model-computed high-level features. Finally, work on clustering for operating mode discovery motivated some important extensions to the clustering strategy we had used. One modification appends an iterative optimization technique onto the clustering system; this optimization strategy appears to be novel in the clustering literature. A second modification improves the noise tolerance of the clustering system. In particular, we adapt resampling-based pruning strategies used by supervised learning systems to the task of simplifying hierarchical clusterings, thus making post-clustering analysis easier.

Fisher, Douglas H.↗
NASA NTRS1994

The Electronic Documentation Project in the NASA mission control center environment

NASA's space programs like many other technical programs of its magnitude is supported by a large volume of technical documents. These documents are not only diverse but also abundant. Management, maintenance, and retrieval of these documents is a challenging problem by itself; but, relating and cross-referencing this wealth of information when it is all on a medium of paper is an even greater challenge. The Electronic Documentation Project (EDP) is to provide an electronic system capable of developing, distributing and controlling changes for crew/ground controller procedures and related documents. There are two primary motives for the solution. The first motive is to reduce the cost of maintaining the current paper based method of operations by replacing paper documents with electronic information storage and retrieval. And, the other is to improve the efficiency and provide enhanced flexibility in document usage. Initially, the current paper based system will be faithfully reproduced in an electronic format to be used in the document viewing system. In addition, this metaphor will have hypertext extensions. Hypertext features support basic functions such as full text searches, key word searches, data retrieval, and traversal between nodes of information as well as speeding up the data access rate. They enable related but separate documents to have relationships, and allow the user to explore information naturally through non-linear link traversals. The basic operational requirements of the document viewing system are to: provide an electronic corollary to the current method of paper based document usage; supplement and ultimately replace paper-based documents; maintain focused toward control center operations such as Flight Data File, Flight Rules and Console Handbook viewing; and be available NASA wide.

Wang, Lui↗
NASA NTRS1997

Design Protocols and Analytical Strategies that Incorporate Structural Reliability Models

Ceramic matrix composites (CMC) and intermetallic materials (e.g., single crystal nickel aluminide) are high performance materials that exhibit attractive mechanical, thermal, and chemical properties. These materials are critically important in advancing certain performance aspects of gas turbine engines. From an aerospace engineers perspective the new generation of ceramic composites and intermetallics offers a significant potential for raising the thrust/weight ratio and reducing NO(sub x) emissions of gas turbine engines. These aspects have increased interest in utilizing these materials in the hot sections of turbine engines. However, as these materials evolve and their performance characteristics improve a persistent need exists for state-of-the-art analytical methods that predict the response of components fabricated from CMC and intermetallic material systems. This need provided the motivation for the technology developed under this research effort. Continuous ceramic fiber composites exhibit an increase in work of fracture, which allows for 'graceful' rather than catastrophic failure. When loaded in the fiber direction these composites retain substantial strength capacity beyond the initiation of transverse matrix cracking despite the fact that neither of its constituents would exhibit such behavior if tested alone. As additional load is applied beyond first matrix cracking, the matrix tends to break in a series of cracks bridged by the ceramic fibers. Any additional load is born increasingly by the fibers until the ultimate strength of the composite is reached. Thus modeling efforts supported under this research effort have focused on predicting this sort of behavior. For single crystal intermetallics the issues that motivated the technology development involved questions relating to material behavior and component design. Thus the research effort supported by this grant had to determine the statistical nature and source of fracture in a high strength, NiAl single crystal turbine blade material; map a simplistic future strength envelope of the material; develop a statistically based reliability computer algorithm; verify the reliability model and computer algorithm-, and model stator vanes for rig tests. Thus establishing design protocols that enable the engineer to analyze and predict the mechanical behavior of ceramic composites and intermetallics would mitigate the prototype (trial and error) approach currently used by the engineering community. The primary objective of the research effort supported by this short term grant is the continued creation of enabling technologies for the macro-analysis of components fabricated from ceramic composites and intermetallic material systems. The creation of enabling technologies aids in shortening the product development cycle of components fabricated from the new high technology materials.

Duffy, Stephen F.↗
NASA NTRS1997

Design Protocols and Analytical Strategies that Incorporate Structural Reliability Models

Ceramic matrix composites (CMC) and intermetallic materials (e.g., single crystal nickel aluminide) are high performance materials that exhibit attractive mechanical, thermal and chemical properties. These materials are critically important in advancing certain performance aspects of gas turbine engines. From an aerospace engineer's perspective the new generation of ceramic composites and intermetallics offers a significant potential for raising the thrust/weight ratio and reducing NO(x) emissions of gas turbine engines. These aspects have increased interest in utilizing these materials in the hot sections of turbine engines. However, as these materials evolve and their performance characteristics improve a persistent need exists for state-of-the-art analytical methods that predict the response of components fabricated from CMC and intermetallic material systems. This need provided the motivation for the technology developed under this research effort. Continuous ceramic fiber composites exhibit an increase in work of fracture, which allows for "graceful" rather than catastrophic failure. When loaded in the fiber direction, these composites retain substantial strength capacity beyond the initiation of transverse matrix cracking despite the fact that neither of its constituents would exhibit such behavior if tested alone. As additional load is applied beyond first matrix cracking, the matrix tends to break in a series of cracks bridged by the ceramic fibers. Any additional load is born increasingly by the fibers until the ultimate strength of the composite is reached. Thus modeling efforts supported under this research effort have focused on predicting this sort of behavior. For single crystal intermetallics the issues that motivated the technology development involved questions relating to material behavior and component design. Thus the research effort supported by this grant had to determine the statistical nature and source of fracture in a high strength, NiAl single crystal turbine blade material; map a simplistic failure strength envelope of the material; develop a statistically based reliability computer algorithm, verify the reliability model and computer algorithm, and model stator vanes for rig tests. Thus establishing design protocols that enable the engineer to analyze and predict the mechanical behavior of ceramic composites and intermetallics would mitigate the prototype (trial and error) approach currently used by the engineering community. The primary objective of the research effort supported by this short term grant is the continued creation of enabling technologies for the macroanalysis of components fabricated from ceramic composites and intermetallic material systems. The creation of enabling technologies aids in shortening the product development cycle of components fabricated from the new high technology materials.

Duffy, Stephen F.↗
NASA NTRS1999

A Quasi-Linear Behavioral Model and an Application to Self-Directed Learning

A model is presented that describes the relationship between one's knowledge of the world and the concomitant personal behaviors that serve as a mechanism to obtain desired outcomes. Integrated within this model are the differing roles that outcomes serve as motivators and as modifiers to one's worldview. The model is dichotomized between general and contextual applications. Because learner self-directedness (a personal characteristic) involves cognition and affection while self-directed learning (a pedagogic process) encompasses conation, behavior and introspection, the model can be dichotomized again in another direction. Presented also are the roles that cognitive motivation theories play in moving an individual through this behavioral model and the roles of wishes, self-efficacy, opportunity and self-influence.

Ponton, Michael K.↗
NASA NTRS1999

Project VISION (Very Intensive Scientific Intercurricular On-Site Education

Project VISION (Very Intensive Scientific Intercurricular On-Site Education) is a joint effort among NASA/John F. Kennedy Space Center, Florida International University, Universidad del Turabo, Miami-Dade County Public Schools and the Caguas/Gurabo Public Schools in Puerto Rico. The project's main mission is to institutionalize change among the elementary and middle school science and math teachers at participating schools so that their students receive continuously enriched instruction in the principles of science and math through the use of hands-on and minds-on experiments called learning modules. These leaming modules incorporate the national science and math education standards provided by the National Committee on Science Education Standards and Assessments and the National Council of Teachers of Mathematics, respectively. The use of learning modules that require hands-on and minds-on activities in a classroom setting garners great enthusiasm and motivation on the part of the target students for the understanding of the lesson's underlying math and science principles. With this enthusiasm and motivation, comes acceptance, attention, participation, discipline, acquiescence, and collaboration. Additionally, the use of hands-on activities may also require learning through a gamut of senses. Not only can the student use his/her eyes and ears during these activities, but most times, they can also use their senses of touch, smell, and taste, as well as intuition. Learning is, therefore, achieved using most or all the human senses. The combination of motivation/enthusiasm and the use of multiple senses creates an ideal environment conducive to leaming at a profound level.

Roig, Gustavo A.↗
NASA NTRS1977

Transport in Halobacterium Halobium: Light-Induced Cation-Gradients, Amino Acid Transport Kinetics, and Properties of Transport Carriers

Cell envelope vesicles prepared from H. halobium contain bacteriorhodopsin and upon illumination protons are ejected. Coupled to the proton motive force is the efflux of Na(+). Measurements of Na-22 flux, exterior pH change, and membrane potential, Delta(psi) (with the dye 3,3'-dipentyloxadicarbocyanine) indicate that the means of Na(+) transport is sodium/proton exchange. The kinetics of the pH changes and other evidence suggests that the antiport is electrogenic (H(+)/Na(++ greater than 1). The resulting large chemical gradient for Na(+) (outside much greater than inside), as well as the membrane potential, will drive the transport of 18 amino acids. The I9th, glutamate, is unique in that its accumulation is indifferent to Delta(psi): this amino acid is transported only when a chemical gradient for Na(+) is present. Thus, when more and more NaCl is included in the vesicles glutamate transport proceeds with longer and longer lags. After illumination the gradient of H+() collapses within 1 min, while the large Na(+) gradient and glutamate transporting activity persists for 10- 15 min, indicating that proton motive force is not necessary for transport. A chemical gradient of Na(+), arranged by suspending vesicles loaded with KCl in NaCl, drives glutamate transport in the dark without other sources of energy, with V(sub max) and K(sub m) comparable to light-induced transport. These and other lines of evidence suggest that the transport of glutamate is facilitated by symport with Na(+), in an electrically neutral fashion, so that only the chemical component of the Na(+) gradient is a driving force.

Lanyi, Janos K.↗