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At least 163 records · Page 9

Absolute measures of the completeness of the fossil record

Measuring the completeness of the fossil record is essential to understanding evolution over long timescales, particularly when comparing evolutionary patterns among biological groups with different preservational properties. Completeness measures have been presented for various groups based on gaps in the stratigraphic ranges of fossil taxa and on hypothetical lineages implied by estimated evolutionary trees. Here we present and compare quantitative, widely applicable absolute measures of completeness at two taxonomic levels for a broader sample of higher taxa of marine animals than has previously been available. We provide an estimate of the probability of genus preservation per stratigraphic interval, and determine the proportion of living families with some fossil record. The two completeness measures use very different data and calculations. The probability of genus preservation depends almost entirely on the Palaeozoic and Mesozoic records, whereas the proportion of living families with a fossil record is influenced largely by Cenozoic data. These measurements are nonetheless highly correlated, with outliers quite explicable, and we find that completeness is rather high for many animal groups.

Non-NASA Center↗

Molecular evolution inferred from small subunit rRNA sequences: what does it tell us about phylogenetic relationships and taxonomy of the parabasalids?

The Parabasala are a primitive group of protists divided into two classes: the trichomonads and the hypermastigids. Until recently, phylogeny and taxonomy of parabasalids were mainly based on the comparative analysis of morphological characters primarily linked to the development of their cytoskeleton. Recent use of molecular markers, such as small subunit (SSU) rRNA has led to now insights into the systematics of the Parabasala and other groups of prolists. An updated phylogeny based on SSU rRNA is provided and compared to that inferred from ultrastructural data. The SSU rRNA phylogeny contradicts the dogma equating simple characters with pumitive characters. Hypermastigids, possessing a hyperdeveloped cytoskeleton, exhibit the most basal emergence in the parabasalid lineage. Other observations emerge from the SSU rRNA analysis, such as the secondary loss of some cytoskeleton structures in all representatives of the Monocercomonadidae, the existence of secondarily free living taxa (reversibility of parasitism) and the evidence against the co-evolution of the endobiotic parabasalids and their animal hosts. According to phylogenies based on SSU rRNA, all the trichomonad families are not monophyletic groups, putting into question the validity of current taxonomic assignments. The precise branching order of some taxa remains unclear, but this issue can possibly be addressed by the molecular analysis of additional parabasalids. The goal of such additional analyses would be to propose, in a near future, a revision of the taxonomy of this group of protists that takes into account both molecular and morphological data.

NASA Discipline Exobiology↗

Evolutionary and preservational constraints on origins of biologic groups: divergence times of eutherian mammals

Some molecular clock estimates of divergence times of taxonomic groups undergoing evolutionary radiation are much older than the groups' first observed fossil record. Mathematical models of branching evolution are used to estimate the maximal rate of fossil preservation consistent with a postulated missing history, given the sum of species durations implied by early origins under a range of species origination and extinction rates. The plausibility of postulated divergence times depends on origination, extinction, and preservation rates estimated from the fossil record. For eutherian mammals, this approach suggests that it is unlikely that many modern orders arose much earlier than their oldest fossil records.

Non-NASA Center↗

Decoupled temporal patterns of evolution and ecology in two post-Paleozoic clades

Counts of taxonomic diversity are the prevailing standards for documenting large-scale patterns of evolution in the fossil record. However, the secular pattern of relative ecological importance between the bryozoan clades Cyclostomata and Cheilostomata is not reflected fully in compilations of generic diversity or within-fauna species richness, and the delayed ecological recovery of the Cheilostomata after the mass extinction at the Cretaceous-Tertiary boundary is missed entirely. These observations demonstrate that evolutionary success and ecological dominance can be decoupled and profoundly different, even over tens of millions of years.

Non-NASA Center↗

A 5.8S nuclear ribosomal RNA gene sequence database: applications to ecology and evolution

We complied a 5.8S nuclear ribosomal gene sequence database for animals, plants, and fungi using both newly generated and GenBank sequences. We demonstrate the utility of this database as an internal check to determine whether the target organism and not a contaminant has been sequenced, as a diagnostic tool for ecologists and evolutionary biologists to determine the placement of asexual fungi within larger taxonomic groups, and as a tool to help identify fungi that form ectomycorrhizae.

Databases, Factual↗

Disparate rates, differing fates: tempo and mode of evolution changed from the Precambrian to the Phanerozoic

Over the past quarter century, detailed genus- and species-level similarities in cellular morphology between described taxa of Precambrian microfossils and extant cyanobacteria have been noted and regarded as biologically and taxonomically significant by numerous workers world-wide. Such similarities are particularly well documented for members of the Oscillatoriaceae and Chroococcaceae, the two most abundant and widespread Precambrian cyanobacterial families. For species of two additional families, the Entophysalidaceae and Pleurocapsaceae, species-level morphologic similarities are supported by in-depth fossil-modern comparisons of environment, taphonomy, development, and behavior. Morphologically and probably physiologically as well, such cyanobacterial "living fossils" have exhibited an extraordinarily slow (hypobradytelic) rate of evolutionary change, evidently a result of the broad ecologic tolerance characteristic of many members of the group and a striking example of G. G. Simpson's [Simpson, G.G. (1944) Tempo and Mode in Evolution (Columbia Univ. Press, New York)] "rule of the survival of the relatively unspecialized." In both tempo and mode of evolution, much of the Precambrian history of life--that dominated by microscopic cyanobacteria and related prokaryotes--appears to have differed markedly from the more recent Phanerozoic evolution megascopic, horotelic, adaptationally specialized eukaryotes.

Review↗

Phylogeny of the ammonia-producing ruminal bacteria Peptostreptococcus anaerobius, Clostridium sticklandii, and Clostridium aminophilum sp. nov

In previous studies, gram-positive bacteria which grew rapidly with peptides or an amino acid as the sole energy source were isolated from bovine rumina. Three isolates, strains C, FT (T = type strain), and SR, were considered to be ecologically important since they produced up to 20-fold more ammonia than other ammonia-producing ruminal bacteria. On the basis of phenotypic criteria, the taxonomic position of these new isolates was uncertain. In this study, the 16S rRNA sequences of these isolates and related bacteria were determined to establish the phylogenetic positions of the organisms. The sequences of strains C, FT, and SR and reference strains of Peptostreptococcus anaerobius, Clostridium sticklandii, Clostridium coccoides, Clostridium aminovalericum, Acetomaculum ruminis, Clostridium leptum, Clostridium lituseburense, Clostridium acidiurici, and Clostridium barkeri were determined by using a modified Sanger dideoxy chain termination method. Strain C, a large coccus purported to belong to the genus Peptostreptococcus, was closely related to P. anaerobius, with a level of sequence similarity of 99.6%. Strain SR, a heat-resistant, short, rod-shaped organism, was closely related to C. sticklandii, with a level of sequence similarity of 99.9%. However, strain FT, a heat-resistant, pleomorphic, rod-shaped organism, was only distantly related to some clostridial species and P. anaerobius. On the basis of the sequence data, it was clear that strain FT warranted designation as a separate species. The closest known relative of strain FT was C. coccoides (level of similarity, only 90.6%). Additional strains that are phenotypically similar to strain FT were isolated in this study.(ABSTRACT TRUNCATED AT 250 WORDS).

NASA Program Exobiology↗

How close is close: 16S rRNA sequence identity may not be sufficient to guarantee species identity

16S rRNA (genes coding for rRNA) sequence comparisons were conducted with the following three psychrophilic strains: Bacillus globisporus W25T (T = type strain) and Bacillus psychrophilus W16AT, and W5. These strains exhibited more than 99.5% sequence identity and within experimental uncertainty could be regarded as identical. Their close taxonomic relationship was further documented by phenotypic similarities. In contrast, previously published DNA-DNA hybridization results have convincingly established that these strains do not belong to the same species if current standards are used. These results emphasize the important point that effective identity of 16S rRNA sequences is not necessarily a sufficient criterion to guarantee species identity. Thus, although 16S rRNA sequences can be used routinely to distinguish and establish relationships between genera and well-resolved species, very recently diverged species may not be recognizable.

Non-NASA Center↗

Rapid recovery from the Late Ordovician mass extinction

Understanding the evolutionary role of mass extinctions requires detailed knowledge of postextinction recoveries. However, most models of recovery hinge on a direct reading of the fossil record, and several recent studies have suggested that the fossil record is especially incomplete for recovery intervals immediately after mass extinctions. Here, we analyze a database of genus occurrences for the paleocontinent of Laurentia to determine the effects of regional processes on recovery and the effects of variations in preservation and sampling intensity on perceived diversity trends and taxonomic rates during the Late Ordovician mass extinction and Early Silurian recovery. After accounting for variation in sampling intensity, we find that marine benthic diversity in Laurentia recovered to preextinction levels within 5 million years, which is nearly 15 million years sooner than suggested by global compilations. The rapid turnover in Laurentia suggests that processes such as immigration may have been particularly important in the recovery of regional ecosystems from environmental perturbations. However, additional regional studies and a global analysis of the Late Ordovician mass extinction that accounts for variations in sampling intensity are necessary to confirm this pattern. Because the record of Phanerozoic mass extinctions and postextinction recoveries may be compromised by variations in preservation and sampling intensity, all should be reevaluated with sampling-standardized analyses if the evolutionary role of mass extinctions is to be fully understood.

Evolution↗

Detailed Mineralogical Characterizations of Four S-Asteroids: 138 Tolosa, 306 Unitas, 346 Hermentaria, and 480 Hansa

The S-asteroid taxonomic class is the second largest grouping of main-belt asteroids that are primarily located in the inner main belt (a < 3.0 AU) [1]. These asteroids have historically been the subject of interest within the asteroid community for several reasons. Continuing discussions about the nature of the S-asteroids (ordinary chondrite-like or thermally-evolved) and the putative space weathering effect on S-asteroids serve as examples of issues currently unresolved within the community [2,3]. Despite this general interest, detailed mineralogical investigations of individual S-asteroids has been relatively rare. A few workers have studied individual, or small groups of, S-asteroids [4,5,6,7]. Gaffey et al. [2] published their S-asteroid survey in 1993 that characterized 39 of the 144 then-classified S-asteroids. Despite the work already accomplished, the need exists to rigorously characterize the remaining S-asteroid population to gain a better understanding of these asteroids origin, nature, and physical characteristics.

Hardersen, P. S.↗

An Automated Flying-Insect-Detection System

An automated flying-insect-detection system (AFIDS) was developed as a proof-of-concept instrument for real-time detection and identification of flying insects. This type of system has use in public health and homeland security decision support, agriculture and military pest management, and/or entomological research. Insects are first lured into the AFIDS integrated sphere by insect attractants. Once inside the sphere, the insect's wing beats cause alterations in light intensity that is detected by a photoelectric sensor. Following detection, the insects are encouraged (with the use of a small fan) to move out of the sphere and into a designated insect trap where they are held for taxonomic identification or serological testing. The acquired electronic wing beat signatures are preprocessed (Fourier transformed) in real-time to display a periodic signal. These signals are sent to the end user where they are graphically displayed. All AFIDS data are pre-processed in the field with the use of a laptop computer equipped with LABVIEW. The AFIDS software can be programmed to run continuously or at specific time intervals when insects are prevalent. A special DC-restored transimpedance amplifier reduces the contributions of low-frequency background light signals, and affords approximately two orders of magnitude greater AC gain than conventional amplifiers. This greatly increases the signal-to-noise ratio and enables the detection of small changes in light intensity. The AFIDS light source consists of high-intensity Al GaInP light-emitting diodes (LEDs). The AFIDS circuitry minimizes brightness fluctuations in the LEDs and when integrated with an integrating sphere, creates a diffuse uniform light field. The insect wing beats isotropically scatter the diffuse light in the sphere and create wing beat signatures that are detected by the sensor. This configuration minimizes variations in signal associated with insect flight orientation.

Vann, Timi↗

Relating practitioner needs to research activities

We present an approach to matching needs (practioner requirements) to solutions (researcher activities). A taxonomical classification scheme acts as intermediary between needs and activities. Expert practitioners exprss their needs in terms of this taxonomy. Researchers express their activities in the same terms. A decision support tool is used to assist in the combination and study of their expressions of needs and activities.

technology transfer requirements prioritization↗

Matching software practitioner needs to researcher activities

We present an approach to matching software practitioners' needs to software researchers' activities. It uses an accepted taxonomical software classfication scheme as intermediary, in terms of which practitioners express needs, and researchers express activities.

technology transfer requirements prioritization↗

Radar Detection of Near-Earth Asteroids 2062 Aten, 2101 Adonis, 3103 Eger, 4544 Xanthus, and 1992 QN

We describe Doppler-only radar observations of near-Earth asteroids 2062 Aten, 2101 Adonis, 3103 Eger, 4544 Xanthus, and 1992 QN that were obtained at Arecibo and Goldstone between 1984 and 1996. Estimates of the echo spectral bandwidths, radar cross sections, and circular polarization ratios of these objects constrain their pole-on breadths, radar albedos, surface roughnesses, taxonomic classes, rotation periods, and spin vectors.

asteroids radar Aten Adonis Eger Xanthus QN↗

The Molecular Ecology of Guerrero Negro: Justifying the Need for Environmental Genomics

The record of life on the only planet where it is known to exist is contained in the biogeochemical processes that organisms catalyze for their survival, in the compounds that they produce, and in their phylogenetic (evolutionary) relationships to each other. We manipulated sulfate and nutrient concentrations in intact microbial mats over periods of time up to a year. The objectives of the manipulations were: 1) characterize the diversity of process-associated functional genes; 2) understand environmental conditions leading to shifts in microbial guilds; 3) monitor/identify competitive responses of organisms sharing a metabolic niche. Characterization of functional genes associated with carbon (mcrA), nitrogen (nifH, nirK) and sulfur (dsrkB) cycling performed to date provided insight into the diversity and metabolic potential of the system; however, we only identified broad scale correlations between gene abundances and changes in mat physiology. For instance, increases in methane production by mats subjected to lowered sulfate and salinity concentrations were correlated with an observed increase in abundance of hydrogenotroph-like mcrA genes. However, due to low sequence similarity to any cultured isolates, phylogenetic associations only allow order level taxonomic commentary, preventing any associations being made on the cellular level. In each of the genes characterized from these experiments, a significant portion of sequences recovered show minimal phylogenetic affiliation to cultured organisms, preventing any understanding of inter-community dynamics and the functional capacities of these unknown organisms. Environmental genomics may provide insight into mat systems by allowing the correlation of functional genes with phylogenetic markers.

Smith, Jason M.↗

Novel Species of Non-Spore-Forming Bacteria

While cataloging cultivatable microbes from the airborne biological diversity of the atmosphere of the Regenerative Enclosed life-support Module Simulator (REMS) system at Marshall Space Flight Center, two strains that belong to one novel bacterial species were isolated. Based on 16S rRNA gene sequencing and the unique morphology and the taxonomic characteristics of these strains, it is shown that they belong to the family Intrasporangiaceae, related to the genus Tetrasphaera, with phylogenetic distances from any validly described species of the genus Tetrasphaera ranging from 96.71 to 97.76 percent. The fatty acid profile supported the affiliation of these novel strains to the genus Tetrasphaera except for the presence of higher concentrations of octadecenoic acid (C18:0) and cis-9-octadecenoic acid (C18:1), which discriminates these strains from other valid species. In addition, DNA-DNA hybridization studies indicate that these strains belong to a novel species that could be readily distinguished from its nearest neighbor, Tetrasphaera japonica AMC 5116T, with less than 20 percent DNA relatedness. Physiological and biochemical tests show few phenotypic dissimilarities, but genotypic analysis allowed the differentiation of these gelatin-liquefying strains from previously reported strains. The name Tetrasphaera remsis sp. Nov. is proposed with the type strain 3-M5-R-4(sup T) (=ATCC BAA-1496(sup T)=CIP 109413(sup T). The cells are Gram-positive, nonmotile, cocci, in tetrad arrangement and clusters. Spore formation is not observed. No species of Tetrashpaera has ever been isolated from airborne samples. Previous discoveries have come from soil and activated sludge samples. As other species of this genus have demonstrated enhanced biological phosphorus removal activity, further tests are required to determine if this newly discovered species would have bioremediation applications.

Briegel, Ariane↗

NEOCAM: Near Earth Object Chemical Analysis Mission: Bridging the Gulf between Telescopic Observations and the Chemical and Mineralogical Compositions of Asteroids or Diogenes A: Diagnostic Observation of the Geology of Near Earth Spectrally-Classified Asteroids

Studies of meteorites have yielded a wealth of scientific information based on highly detailed chemical and isotopic studies possible only in sophisticated terrestrial laboratories. Telescopic studies have revealed an enormous (greater than 10(exp 5)) number of physical objects ranging in size from a few tens of meters to several hundred kilometers, orbiting not only in the traditional asteroid belt between Mars and Jupiter but also throughout the inner solar system. Many of the largest asteroids are classed into taxonomic groups based on their observed spectral properties and are designated as C, D. X, S or V types (as well as a wide range in sub-types). These objects are certainly the sources far the meteorites in our laboratories, but which asteroids are the sources for which meteorites? Spectral classes are nominally correlated to the chemical composition and physical characteristics of the asteroid itself based on studies of the spectral changes induced in meteorites due to exposure to a simulated space environment. While laboratory studies have produced some notable successes (e.g. the identification of the asteroid Vesta as the source of the H, E and D meteorite classes), it is unlikely that we have samples of each asteroidal spectral type in our meteorite collection. The correlation of spectral type and composition for many objects will therefore remain uncertain until we can return samples of specific asteroid types to Earth for analyses. The best candidates for sample return are asteroids that already come close to the Earth. Asteroids in orbit near 1 A.U. have been classified into three groups (Aten, Apollo & Amor) based on their orbital characteristics. These Near Earth Objects (NEOs) contain representatives of virtually all spectral types and sub-types of the asteroid population identified to date. Because of their close proximity to Earth, NEOs are prime targets for asteroid missions such as the NEAR-Shoemaker NASA Discovery Mission to Eros and the Japanese Hyabusa Mission to Itokawa. Also due to their close proximity to Earth, NEOs constitute the most likely set of celestial objects that will impact us in the relatively near future.

Nuth, Joseph A.↗

An Automated Flying-Insect Detection System

An automated flying-insect detection system (AFIDS) was developed as a proof-of-concept instrument for real-time detection and identification of flying insects. This type of system has use in public health and homeland-security decision support, agriculture and military pest management, and/or entomological research. Insects are first lured into the AFIDS integrated sphere by insect attractants. Once inside the sphere, the insect s wing beats cause alterations in light intensity that is detected by a photoelectric sensor. Following detection, the insects are encouraged (with the use of a small fan) to move out of the sphere and into a designated insect trap where they are held for taxonomic identification or serological testing. The acquired electronic wing-beat signatures are preprocessed (Fourier transformed) in real time to display a periodic signal. These signals are sent to the end user where they are graphically. All AFIDS data are preprocessed in the field with the use of a laptop computer equipped with LabVIEW. The AFIDS software can be programmed to run continuously or at specific time intervals when insects are prevalent. A special DC-restored transimpedance amplifier reduces the contributions of low-frequency background light signals, and affords approximately two orders of magnitude greater AC gain than conventional amplifiers. This greatly increases the signal-to-noise ratio and enables the detection of small changes in light intensity. The AFIDS light source consists of high-intensity Al-GaInP light-emitting diodes (LEDs). The AFIDS circuitry minimizes brightness fluctuations in the LEDs and when integrated with an integrating sphere, creates a diffuse uniform light field. The insect wing beats isotropically scatter the diffuse light in the sphere and create wing-beat signatures that are detected by the sensor. This configuration minimizes variations in signal associated with insect flight orientation. Preliminary data indicate that AFIDS has sufficient sensitivity and frequency measuring capability to differentiate between male and female mosquitoes (Figure 1, bottom panel) and fruit flies (data not shown). Similar studies show that AFIDS can be utilized to detect discrete differences between two mosquito species, Aedes aegypti and Aedes albopictus. When fully deployable, a wireless network of AFIDS monitors could be used in combination with other remotely sensed data and visually displayed in a geographic information system (GIS) to provide real-time surveillance (see Figure 2). More accurate and sensitive insect population forecasts and effective rapid response and mitigation of insect issues would then be possible.

Vann, Timi↗