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At least 163 records · Page 9

Utilization of non-conventional systems for conversion of biomass to food components

Described here is work accomplished in investigating the potential use of micro-algae in yielding useful macronutrients for closed ecological life support systems in space habitats. Analysis of the chemical composition of the blue-green alga Synechoccus 6311 was done in the present work, and was compared to values found in previous work on the green algae Scenedesmus obliquus. Similar values were obtained for proteins, and lower values for nucleic acids and lipids. A second part of the work involved fabrication of food products containing various levels of incorporated algae (S. obliquus) proteins and/or lipids. Protein isolate was incorporated into a variety of food products such as bran muffins, fettuccine (spinach noodle imitation), and chocolate chip cookies. In the sensory analysis, the greenish color of the bran muffins and cookies was not found to be objectionable. The mild spinachy flavor was less detectable in chocolate chip cookies than in bran muffins. The color and taste of the algae noodles were found to be pleasant and compared well with commercially available spinach noodles.

Karel, M.↗

Observations on gas exchange and element recycle within a gas-closed algal-mouse system

Life support systems based on bioregeneration rely on the control and manipulation of organisms. Algae are potentially useful for a variety of Closed Ecological Life Support System (CELSS) functions including the revitalization of atmospheres, production of food and for nitrogen fixation. The results of experiments conducted with a gas-closed algal-mouse system designed to investigate gas exchange phenomena under varying algal environmental conditions, and the ability of algae to utilize oxidized mouse solid waste are reported. Inherent instabilities exist between the uptake and release of carbon dioxide (CO2) and oxygen (O2) by the mouse and algae in a gas-closed system. Variations in light intensity and cell density alter the photosynthetic rate of the algae and enable short-term steady-state concentrations of atmospheric CO2 and O2. Different nitrogen sources (urea and nitrate) result in different algal assimilatory quotients (AQ). Combinations of photosynthetic rate and AQ ratio manipulations were examined for their potential in stabilizing atmospheric gas concentrations in the gas-closed algal-mouse system.

Smernoff, D. T.↗

Algal Accessory Pigment Detection Using AVIRIS Image-Derived Spectral Radiance Data

Visual and derivative analyses of AVIRIS spectral data can be used to detect algal accessory pigments in aquatic communities. This capability extends the use of remote sensing for the study of aquatic ecosystems by allowing detection of taxonomically significant pigment signatures which yield information about the type of algae present. Such information allows remote sensing-based assessment of aquatic ecosystem health, as in the detection of nuisance blooms of cyanobacteria or toxic blooms of dinoflagellates. Remote sensing of aquatic systems has traditionally focused on quantification of chlorophyll a, a photoreactive (and light-harvesting) pigment which is common to all algae as well as cyanobacteria (bluegreen algae). Due to the ubiquitousness of this pigment within algae, chl a is routinely measured to estimate algal biomass both during ground-truthing and using various airborne or satellite based sensors, including AVIRIS. Within the remote sensing and aquatic sciences communities, ongoing research has been performed to detect algal accessory pigments for assessment of algal population composition. This research is based on the fact that many algal accessory pigments are taxonomically significant, and all are spectrally unique. Aquatic scientists have been refining pigment analysis techniques, primarily high performance liquid chromatography, or HPLC, to detect specific pigments as a time-saving alternative to individual algal cell identifications and counts. Remote sensing scientists are investigating the use of pigment signatures to construct pigment libraries analogous to mineral spectral libraries used in geological remote sensing applications. The accessory pigment approach has been used successfully in remote sensing using data from the Thematic Mapper, low-altitude, multiple channel scanners, field spectroradiometers and the AVIRIS hyperspectral scanner. Due to spectral and spatial resolution capabilities, AVIRIS is the sensor of choice for such studies. We present here our results on detection of algal accessory pigments using AVIRIS data.

Richardson, Laurie L.↗

Method of producing purified carotenoid compounds

A method of producing a carotenoid in solid form includes culturing a strain of Chlorophyta algae cells in a minimal inorganic medium and separating the algae comprising a solid form of carotenoid. In one embodiment f the invention, the strain of Chlorophyta algae cells includes a strain f Chlamydomonas algae cells.

Eggink, Laura↗

Quantifying Spatiotemporal Variability of Glacier Algal Blooms and the Impact on Surface Albedo in Southwestern Greenland

Albedo reduction due to light-absorbing impurities can substantially enhance ice sheet surface melt by increasing surface absorption of solar energy. Glacier algae have been suggested to play a critical role in darkening the ablation zone in southwestern Greenland. It was very recently found that the Sentinel-3 Ocean and Land Colour Instrument (OLCI) band ratio R709 nm∕R673 nm can characterize the spatial patterns of glacier algal blooms. However, Sentinel-3 was launched in 2016, and current data are only available over three melting seasons (2016–2019). Here, we demonstrate the capability of the MEdium Resolution Imaging Spectrometer (MERIS) for mapping glacier algae from space and extend the quantification of glacier algal blooms over southwestern Greenland back to the period 2004–2011. Several band ratio indices (MERIS chlorophyll a indices and the impurity index) were computed and compared with each other. The results indicate that the MERIS two-band ratio index (2BDA) R709 nm∕R665 nm is very effective in capturing the spatial distribution and temporal dynamics of glacier algal growth on bare ice in July and August. We analyzed the interannual (2004–2011) and summer (July–August) trends of algal distribution and found significant seasonal and interannual increases in glacier algae close to the Jakobshavn Isbrae Glacier and along the middle dark zone between the altitudes of 1200 and 1400 m. Using broadband albedo data from the Moderate Resolution Imaging Spectroradiometer (MODIS), we quantified the impact of glacier algal growth on bare ice albedo, finding a significant correlation between algal development and albedo reduction over algae-abundant areas. Our analysis indicates the strong potential for the satellite algal index to be used to reduce bare ice albedo biases in regional climate model simulations.

surface albedo↗

Probable calcified metaphytes in the latest Proterozoic Nama Group, Namibia: origin, diagenesis, and implications

Samples from the Huns Limestone Member, Urusis Formation, Nama Group, at two adjacent localities in southern Namibia contain thin foliose to arched, sheet-like carbonate crusts that are 100-500 micrometers thick and up to 5 cm in lateral dimension. Morphologic, petrographic, and geochemical evidence supports the interpretation of these delicate crusts as biogenic, most likely the remains of calcified encrusting metaphytes. The original sediments of the fossiliferous samples contained aragonitic encrusting algae, botryoidal aragonite cements, and an aragonite mud groundmass. Spherulites within the precursor mud could represent bacterially induced mineral growths or the concretions of marine rivularian cyanobacteria. Original textures were severely disrupted during the diagenetic transition of aragonite to low-magnesian calcite, but some primary structures remain discernible as ghosts in the neomorphic mosaic. Gross morphology, original aragonite mineralogy, and hypobasal calcification indicate that the crusts are similar to late Paleozoic phylloid algae and extant peyssonnelid red algae. Structures interpreted as possible conceptacles also suggest possible affinities with the Corallinaceae. Two species of Cloudina, interpreted as the remains of a shelly metazoan, are also known from limestones in the Nama Group. It is possible, therefore, that skeletalization in metaphytes and animals arose nearly simultaneously near the end of the Proterozoic Eon.

NASA Discipline Number 52-30↗

Semiconductor diode laser material and devices with emission in visible region of the spectrum

Two alloy systems, (AlGa)As and (InGa)P, were studied for their properties relevant to obtaining laser diode operation in the visible region of the spectrum. (AlGa)As was prepared by liquid-phase epitaxy (LPE) and (InGa)P was prepared both by vapor-phase epitaxy and by liquid-phase epitaxy. Various schemes for LPE growth were applied to (InGa)P, one of which was found to be capable of producing device material. All the InGaP device work was done using vapor-phase epitaxy. The most successful devices were fabricated in (AlGa)As using heterojunction structures. At room temperature, the large optical cavity design yielded devices lasing in the red (7000 A). Because of the relatively high threshold due to the basic band structure limitation in this alloy, practical laser diode operation is presently limited to about 7300 A. At liquid-nitrogen temperature, practical continuous-wave operation was obtained at a wavelength of 6500 to 6600 A, with power emission in excess of 50 mW. The lowest pulsed lasing wavelength is 6280 A. At 223 K, lasing was obtained at 6770 A, but with high threshold currents. The work dealing with CW operation at room temperature was successful with practical operation having been achieved to about 7800 A.

Ladany, I.↗

Room-temperature-operation visible-emission semiconductor diode lasers

There were two main approaches taken to develop shorter wavelength lasers. (1) Based on (AlGa)As and liquid-phase epitaxy, significant new results were obtained: Properties of these laser diodes (power output, spectra, and beam patterns), materials considerations, laser theory, and growth problems are discussed. The design of (AlGa)As layers is discussed from the vertical point of view, and various design curves are given. Horizontal structural requirements are also discussed. Experimental results from measurements done as a function of hydrostatic pressure are correlated with other results. (2) The first heterojunction laser structures using GaAs sub l-x P sub x and In sub y Ga sub l-y P at compositions, where the lattice constants are matched, were grown using vapor-phase growth technology and are described in detail, including experimental device results. Threshold current densities from 3,000 to 5,000 A per sq cm. and emission wavelengths from 6,520 A to 6,640 A were obtained at 77 K. The limiting factor in these devices is nonradiative recombination at the heterojunctions. Life tests on facet-coated (AlGa)As CW diodes are reported.

Ladany, I.↗

HCMM hydrological analysis in Utah

Progress in analysis of Heat Capacity Mapping Mission (HCMM) infrared and visible observations of the hydrology of Utah Lake is reported. Correlation between HCMM intensities converted to temperature and ground truth temperatures was investigated, and a conversion offset value determined. Ground truth surface temperatures minus HCMM temperatures were plotted against several hydrological parameters. Relationships among visible data, thermal data, and algae concentrations were considered, and summer concentrations of predominant algae species determined. Investigations on the effects of varying algae concentrations on evaporation rates are reported. Efforts to develop a model for evaporation estimation are reported. The relationship between air and water surface temperatures was studied and the temperature distribution in different segments of the lake investigated. Indications of the existence of thermal springs are reported. Correlation of HCMM surface temperature data and depth to groundwater were investigated.

Source record↗

HCMM hydrological analysis in Utah

The feasibility of applying a linear model to HCMM data in hopes of obtaining an accurate linear correlation was investigated. The relationship among HCMM sensed data surface temperature and red reflectivity on Utah Lake and water quality factors including algae concentrations, algae type, and nutrient and turbidity concentrations was established and evaluated. Correlation (composite) images of day infrared and reflectance imagery were assessed to determine if remote sensing offers the capability of using masses of accurate and comprehensive data in calculating evaporation. The effects of algae on temperature and evaporation were studied and the possibility of using satellite thermal data to locate areas within Utah Lake where significant thermal sources exist and areas of near surface groundwater was examined.

Miller, A. W.↗

The effect of fast and regeneration in light versus dark on regulation in the hydra-algal symbiosis

Green hydra are able to regenerate tentacles after fast durations which cause brown, i.e., asymbiotic, hydra to fail completely, but the presence of endosymbiotic algae does not always enhance regeneration in fasted hydra. Green hydra whose nutritional state falls below some threshold, exhibit a light induced inhibition of regeneration. That is, hydra, fasted in the light, then randomly assigned to light or dark after decapitation, regenerate better in the dark. This effect of light does not appear to be present either in brown hydra or in normally green hydra from which the algae were removed. In a large strain of Chlorohydra viridissima, after fasts of intermediate duration (10 and 15 days), this light induced inhibition of regeneration is associated with an increase in the number of algae per gastric cell in regenerating hydra relative to non-regenerating controls.

Bossert, P.↗

The effect of various carbon sources on the growth of single-celled cyanophyta

In 19 strains of unicellular blue-green algae, belonging to general Synechococcus, Synechocystis, Aphanocapsa and Aphanothece, the capacity of growth under mixotrophic conditions in mineral media with organic carbon sources (carbohydrates, polyols) was investigated. At moderate light intensity (1200 lx) and 0.5% of carbon source there was revealed: (1) Stimulation of growth; (2) Partial or complete inhibition of growth; (3) No influence of carbohydrate and polyols on the growth of some algae strains. Three physiological groups for the investigated strains have been outlined on the basis of data obtained. The possibility of using the differences revealed in classification of unicellular blue-green algae is discussed.

Avilov, I. A.↗

Laser-induced fluorescence of green plants. III - LIF spectral signatures of five major plant types

A technique amenable to remote sensing use which utilizes laser-induced fluorescence (LIF) properties of plants has been successfully used in the laboratory to identify five major plant types. These included herbaceous dicots, herbaceous monocots, conifers, hardwoods, and algae. Each of these plant types exhibited a characteristic LIF spectra when excited by a pulsed N2 laser emitting at 337 nm. Although monocots and dicots possess common fluorescence maxima at 440, 685, and 740 nm, they could be differentiated from one another by using the ratio of the square of the fluorescence intensity at 440 nm to the nonsquared intensity at 685 nm, i.e., (440)-squared/685. In all cases, monocots yielded a significantly higher ratio. Conifers have fluorescence maxima at 440, 525, and 740 nm but none at 685 nm. Hardwoods exhibited fluorescence at 440, 525, 685, and 740 nm. Algae had very low fluorescence at 440 nm, no fluorescence at 525 nm, and fluorescence maxima at 685 and 740 nm. For algae, the ratio of the fluorescence intensity at 685 nm to that at 740 nm was much greater than that for monocots, dicots, and hardwoods. The potential use of the LIF technique for individual species identification is suggested.

Chappelle, E. W.↗

Operation of an experimental algal gas exchanger for use in a CELSS

Concepts of a Closed Ecological Life Support System (CELSS) anticipate the use of photosynthetic organisms (higher plants and algae) for air revitalization. The rates of production and uptake of carbon dioxide and oxygen between the crew and the photosynthetic organisms are mismatched. An algal system used for gas exchange only will have the difficulty of an accumulation or depletion of these gases beyond physiologically tolerable limits (in a closed system the mismatch between assimilatory quotient (AQ) and respiratory quotient (RQ) is balanced by the operation of the waste processor). The results are given of a study designed to test the feasibility of using environmental manipulations to maintain physiologically appropriate atmospheres for algae and mice in a gas closed system. Specifically, the atmosphere behavior of this system is considered with algae grown on nitrate or urea and at different light intensities and optical densities. Manipulation of both allow operation of the system in a gas stable manner. Operation of such a system in a CELSS may be useful for reduction of buffer sizes, as a backup system for higher plant air revitalization and to supply extra oxygen to the waste processor or during crew changes.

Smernoff, David T.↗

Snow as a habitat for microorganisms

There are three major habitats involving ice and snow, and the microorganisms studied from these habitats are most eukaryotic. Sea ice is inhabited by algae called diatoms, glacial ice has sparse populations of green algai cal desmids, and the temporary and permanent snows in mountainous regions and high latitudes are inhabited mostly by green algal flagellates. The life cycle of green algal flagellates is summarized by discussing the effects of light, temperature, nutrients, and snow melts. Specific examples of optimal conditions and environmental effects for various snow algae are given. It is not likely that the eukaryotic snow algae presented are candidated for life on the planet Mars. Evolutionally, eukaryotic cells as know on Earth may not have had the opportunity to develop on Mars (if life evolved at all on Mars) since eukaryotes did not appear on Earth until almost two billion years after the first prokaryotic organisms. However, the snow/ice ecosystems on Earth present themselves as extreme habitats were there is evidence of prokaryotic life (eubacteria and cyanbacteria) of which literally nothing is known. Any future surveillances of extant and/or extinct life on Mars should include probes (if not landing sites) to investigate sites of concentrations of ice water. The possibility of signs of life in Martian polar regions should not be overlooked.

Hoham, Ronald W.↗

Multidimensional computer simulation of Stirling cycle engines

The computer code ALGAE (algorithms for the gas equations) treats incompressible, thermally expandable, or locally compressible flows in complicated two-dimensional flow regions. The solution method, finite differencing schemes, and basic modeling of the field equations in ALGAE are applicable to engineering design settings of the type found in Stirling cycle engines. The use of ALGAE to model multiple components of the space power research engine (SPRE) is reported. Videotape computer simulations of the transient behavior of the working gas (helium) in the heater-regenerator-cooler complex of the SPRE demonstrate the usefulness of such a program in providing information on thermal and hydraulic phenomena in multiple component sections of the SPRE.

Hall, C. A.↗

Multidimensional computer simulation of Stirling cycle engines

This report summarizes the activities performed under NASA-Grant NAG3-1097 during 1991. During that period, work centered on the following tasks: (1) to investigate more effective solvers for ALGAE; (2) to modify the plotting package for ALGAE; and (3) to validate ALGAE by simulating oscillating flow problems similar to those studied by Kurzweg and Ibrahim.

Hall, Charles A.↗

Design of components for the NASA OCEAN project

The goal of the Fall 1993 semester of the EGM 4000 class was to design, fabricate, and test components for the 'Ocean CELSS Experimental Analog NASA' Project (OCEAN Project) and to aid in the future development of NASA's Controlled Ecological Life Support System (CELSS). The OCEAN project's specific aims are to place a human, Mr. Dennis Chamberland from NASA's Life Science Division of Research, into an underwater habitat off the shore of Key Largo, FL for three months. During his stay, he will monitor the hydroponic growth of food crops and evaluate the conditions necessary to have a successful harvest of edible food. The specific designs chosen to contribute to the OCEAN project by the EGM 4000 class are in the areas of hydroponic habitat monitoring, human health monitoring, and production of blue/green algae. The hydroponic monitoring system focused on monitoring the environment of the plants. This included the continuous sensing of the atmospheric and hydroponic nutrient solution temperatures. Methods for monitoring the continuous flow of the hydroponic nutrient solution across the plants and the continuous supply of power for these sensing devices were also incorporated into the design system. The human health monitoring system concentrated on continuously monitoring various concerns of the occupant in the underwater living habitat of the OCEAN project. These concerns included monitoring the enclosed environment for dangerous levels of carbon monoxide and smoke, high temperatures from fire, and the ceasing of the continuous airflow into the habitat. The blue/green algae project emphasized both the production and harvest of a future source of food. This project did not interact with any part of the OCEAN project. Rather, it was used to show the possibility of growing this kind of algae as a supplemental food source inside a controlled ecological life support system.

Wright, Jenna↗