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At least 163 records · Page 9

Quantifying uncertainties in greenhouse gas savings and abatement costs with cellulosic biofuels

Cellulosic biofuels from non-food feedstocks, while appealing, continue to encounter uncertainty about their induced land use change (ILUC) effects, net greenhouse gas (GHG) saving potential and their economic costs. We analyze the implications of multiple uncertainties along the biofuel supply chain from feedstock yields, land availability for production to conversion to fuel in the refinery on these outcomes. Furthermore, we find that compared to corn ethanol, cellulosic biofuels have a substantially smaller and less uncertain ILUC-related GHG intensity and lead to larger GHG savings at lower welfare costs of abatement, indicating the potential to make robust and substantial contributions to cost-effective climate change mitigation.

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Biomass feedstock transport using fuel cell and battery electric trucks improves lifecycle metrics of biofuel sustainability and economy

We report the use of new vehicle technologies such as fuel cell hybrid electric and fully electric powertrains for biomass feedstock supply is an unexplored solution to reducing biofuel production cost, greenhouse gas emissions, and health impacts. These technologies have found success in light-duty vehicle applications and are in development for heavy-duty trucks. This study presents the first detailed stochastic techno-economic analysis and life-cycle assessment of biomass feedstock supply systems with diesel, fuel cell hybrid electric, and fully electric trucks and determines their impacts on biofuel production considering butanol as a representative biofuel. This study finds that fuel cell hybrid electric and fully electric trucks consume less energy relative to the diesel-powered truck regardless of the evaluated circumstances, including payloads of truck (loaded and empty), pavement types (gravel and paved), road conditions (normal and damaged), and road networks (local and highways). The use of fuel cell hybrid and fully electric trucks powered by H 2 -fuel and renewable sources of electricity, respectively, results in a large reduction in cost and carbon footprint, specifically for a long-distance hauling, and minimize other economic and environmental impacts. While the economic advantage of fuel cell hybrid electric vehicle is dependent on the price of H 2 -fuel and road conditions, use reduces the GHG emissions of biobutanol per 100 km-trucking-distance by 0.98-10.9 gCO 2e /MJ. Results show that converting to fully electric truck transport decreases the biobutanol production cost and GHG emissions per 100 km-trucking-distance by 0.4-7.3 cents/L and 0.78 to 9.1 gCO 2e /MJ, respectively. This study establishes the foundation for future investigations that will guide the development of economically, socially, and environmentally sustainable biomass feedstock supply system for cellulosic biorefineries or other goods transportation systems.

09 BIOMASS FUELS↗

2024 Bioenergy Industry Status Report

This report provides a snapshot of the bioenergy industry status at the end of 2024. The report compliments other annual market reports from the Department of Energy's (DOE's) Office of Energy Efficiency and Renewable Energy (EERE) offices and is supported by DOE'sOffice of Critical Minerals and Energy Innovation's Alternative Fuels and Feedstocks Office. The 2024 Bioenergy Industry Status Report focuses on past year data covering multiple dimensions of the bioenergy industry and does not attempt to make future market projections. There report covers production, consumption, plants, trade, and end-use for all biofuels, biopower, and biobased products where data is available. The report provides a balanced and unbiased assessment of the industry and associated markets. It is openly available to the public and is intended to complement other industry reports with a focus on DOE stakeholder needs.

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Supply chain system for a centralized biorefinery system based on switchgrass grown on marginal land in Michigan

The feedstock supply chain of a biorefinery is critical in determining the economic and environmental performance of biofuels. This study investigated various feedstock supply chains for a centralized biorefinery based on different objective functions and how the biorefinery responds to supply chain volatility. For the analysis, a hypothetical centralized biorefinery was supplied by switchgrass grown on marginal lands in Michigan. From an economic standpoint, the minimum biofuel selling price is an important metric of the objective function, and the greenhouse gas mitigation potential is a similarly important metric for global warming impact. A trade-off therefore exists between the economic and environmental performance of biofuels under this system configuration. However, when a carbon tax credit is applied to soil organic carbon sequestration in switchgrass production, the metric of the objective function may not be the key factor in establishing a supply chain as long as it is associated with biorefinery capacity as well as economic or environmental values. Year-to-year fluctuations in cellulosic biomass yield (supply chain volatility) might also prevent the biorefinery from operating at full capacity. Cellulosic biomass pellets can serve as an auxiliary feedstock for the centralized cellulosic biorefinery system to minimize the impacts of supply chain volatility.

09 BIOMASS FUELS↗

Deploying Purpose-Grown Energy Crops for Sustainable Aviation Fuel (Workshop Summary Report)

From June 6–7, 2023, the U.S. Department of Energy (DOE) Bioenergy Technologies Office (BETO) hosted the workshop “Deploying Purpose-Grown Energy Crops for Sustainable Aviation Fuel” in Kansas City, Missouri. The purpose of the workshop was to examine topics related to large-scale cultivation of purpose-grown energy crops—including algae—that can contribute feedstocks for the production of biofuels and bioproducts. This workshop was a means to gather a variety of stakeholders with the intent to identify elements for a potential future research strategy designed to overcome challenges and delve into the barriers related to the large-scale cultivation of purpose-grown crops. This document provides an overview and summary of the workshop’s presentations, breakout session discussions, and outcomes.

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Feedstock-Conversion Interface Consortium Annual Review of Research – FY 2021

Led by the U.S. Department of Energy’s Bioenergy Technologies Office, the Feedstock-Conversion Interface Consortium (FCIC) is a multi-lab collaboration that develops first-principles-based knowledge and tools to understand, quantify, and mitigate the effects of feedstock and process variability across the bioenergy value chain, from the field and forest through downstream conversion. The FCIC recently published its Annual Review of Research Fiscal Year (FY) 2021 report, which presents an overview of the research accomplishments of FCIC members in FY 2021, covering both the low- and high-temperature conversion pathways and all three process areas: Feedstock harvest and storage Preprocessing Conversion. The key research achievements of each task are presented, along with planned FY 2022 work that will build on the FY 2021 accomplishments.

09 BIOMASS FUELS↗

A toolkit for plant lipid engineering: surveying the efficacies of lipogenic factors for accumulating specialty lipids

Plants produce energy-dense lipids from carbohydrates using energy acquired via photosynthesis, making plant oils an economically and sustainably attractive feedstock for conversion to biofuels and value-added bioproducts. A growing number of strategies have been developed and optimized in model plants, oilseed crops and high-biomass crops to enhance the accumulation of storage lipids (mostly triacylglycerols, TAGs) for bioenergy applications and to produce specialty lipids with increased uses and value for chemical feedstock and nutritional applications. Most successful metabolic engineering strategies involve heterologous expression of lipogenic factors that outperform those from other sources or exhibit specialized functionality. In this review, we summarize recent progress in engineering the accumulation of triacylglycerols containing - specialized fatty acids in various plant species and tissues. We also provide an inventory of specific lipogenic factors derived from a wide variety of organisms, along with their reported efficacy in supporting the accumulation of desired lipids. A review of previously obtained results serves as a foundation to guide future efforts to optimize combinations of factors to achieve further enhancements to the production and accumulation of desired lipids in a variety of plant tissues and species.

59 BASIC BIOLOGICAL SCIENCES↗

Utilizing data-based modeling with low life cycle GHG emissions algae biofuels for engine optimization

Aquatic microalgae are a highly promising feedstock for the production of biocrude and tailored biofuels, with distinct advantages over traditional terrestrial crops, such as reduced land use and avoidance of food production competition. However, unlocking their full potential requires the development of biofuels with low life cycle greenhouse emissions biofuels, such as algae biofuels, which can significantly reduce the environmental impact of the transportation systems without requiring a complete overhaul of existing engine technology. In this study, we employ cutting-edge data-based AI modeling techniques to optimize the performance of heavy-duty engines, with a focus on transitioning towards biofuels with low life cycle greenhouse emissions biofuels. Our methodology offers significant advantages over traditional sweep testing, enabling efficient and accurate optimization of engine performance with minimal time and resources consumption. Our findings demonstrate the potential of utilizing this approach, with up to 55% NOx emissions reductions and up to 2% reduction in fuel consumption compared to the baseline optimized point. Moving forward, we plan to utilize a 30% blend of algae biofuels with diesel fuel, with the ultimate goal of achieving up to 60% lifecycle GHG emissions. Lastly, we plan to compare the results with 100% renewable biodiesel to add an additional dimension of investigating the impact of fuel chemistry on engine optimization. Overall, this study underscores the vital importance of biofuels for reducing the carbon footprint of the transportation sector and supporting a sustainable future. By harnessing the power of data-based AI modeling with low life cycle greenhouse emissions biofuels, we can accelerate the adoption of more environmentally friendly transportation systems and reduce their impact on the planet. Our findings contribute to this transition and offer insights for developing efficient and effective strategies for addressing global climate change.

09 BIOMASS FUELS↗

Drop-in sustainable aviation fuels enabled by feedstock-agnostic lignin deoxygenation

Current sustainable aviation fuels (SAFs) require blending with petroleum-derived fuels due to incomplete hydrocarbon distributions, most notably a lack of aromatics. Lignin, the most abundant renewable source of aromatics, is a promising feedstock for addressing this limitation. Here, we demonstrate a sequential reductive catalytic fractionation and continuous hydrodeoxygenation process that converts multiple woody feedstocks into aromatic hydrocarbons at up to 93% of the theoretical carbon yield. Blending these products with commercial SAFs produces drop-in compatible fuels with elastomer swell performances equivalent to conventional aviation fuels. The process is adaptable across multiple biomass sources, yielding aromatic hydrocarbons with consistent enthalpic efficiencies and fuel properties. These findings establish a scalable route to 100% drop-in SAFs, leveraging lignin-derived aromatics within the existing biofuels infrastructure.

09 BIOMASS FUELS↗

Triacylglycerol, total fatty acid, and biomass accumulation of metabolically engineered energycane grown under field conditions confirms its potential as feedstock for drop‐in fuel production

Abstract Metabolic engineering for hyperaccumulation of lipids in vegetative tissues of high biomass crops promises a step change in oil yields for the production of advanced biofuels. Energycane is the ideal feedstock for this approach due to its exceptional biomass production and persistence under marginal conditions. Here, we evaluated metabolically engineered energycane with constitutive expression of the lipogenic factors WRINKLED 1 ( WRI 1), DIACYLGLYCEROL ACYLTRANSFERASE 1 ( DGAT 1), and OLEOSIN 1 ( OLE 1) for the accumulation of triacylglycerol (TAG), total fatty acid (TFA), and biomass under field conditions at the University of Florida‐IFAS experiment station near Citra, Florida. TAG and TFA accumulation were highest in leaves (up to 9.9% and 12.9% of DW, respectively), followed by juice from crushed stems, stems, and roots. TAG and TFA accumulation increased up to harvest time and correlated highest with OLE 1 and DGAT 1 expression. Biomass dry weight, TAG, and TFA content differed greatly depending on DGAT 1 and OLE 1 expression in transgenic lines with similar WRI 1 expression. Biomass did not significantly differ between WT and line L2 with DAGT 1 and OLE 1 expressed at low levels and TAG and TFA accumulating to 12‐ and 1.6‐fold that of WT leaves, respectively. In contrast, line L13, with intron‐mediated enhancement of DGAT 1 expression, displayed a 245‐ to 330‐fold increase in TAG and a 4.75‐ to 6.45‐fold increase in TFA content compared with WT leaves and a biomass reduction of 52%. These results provide the basis for developing novel feedstocks for expanding plant lipid production and point to new prospects for advanced biofuels.

59 BASIC BIOLOGICAL SCIENCES↗

Insights into Mechanisms of Screw Feeder Plugging by Heated Pine Forestry Residues Using in-situ and Correlative Microscopy

Increasing concerns about global carbon emissions due to fossil fuel use has created interest in renewable fuel alternatives, such as lignocellulosic biomass. With an estimated global biomass production probability ten-fold higher than projected need, functionalizing and scaling biomass conversion processes, like pyrolysis, is a promising component of a diverse sustainable energy program (Kan, Strezov, & Evans, 2016). Pyrolysis reactors employed in biomass conversion often encounter the known problem of screw feeder plugging, halting work and reducing efficiency of biorefineries. Although the screw feeder is not heated directly, conductive heat transfer from the fluidized bed pyrolysis reactor creates a temperature gradient, where particles experience temperatures between 22°C and 500°C from the time they enter the feeder to being emptied into the reactor (Digman, Joo, & Kim, 2009; Nieminen & Folke, 1982). The impact of unintentional heating on this highly variable feedstock may result in particle morphology modification, surface texture transformation, and the evolution of viscous compounds and may contribute to screw feeder plugging (Haas, Nimlos, & Donohoe, 2009; Ingram et al., 2008). We hypothesized that particles of various anatomical fractions (including needles, branches, bark, cambium, and whitewood) will experience dramatic changes during this heating regime, which will be characterized by decreased particle size, increased surface roughness, degradation of key structural macromolecules, and generation of viscous compounds that would result in increased particle friction and cohesion, contributing to screw feeder plugging.

biofuels↗

Hydrothermal pretreatment for valorization of genetically engineered bioenergy crop for lipid and cellulosic sugar recovery

Lipids accumulated in the vegetative tissues of cellulosic feedstocks can be a potential raw material for biodiesel and bioethanol production. In this work, bagasse of genetically engineered sorghum was subjected to liquid hot-water pretreatment at 170, 180, and 190°C for different reaction time. Under the optimal pretreatment condition (170°C, 20 min), the residue was enriched in glucan (57.39 ± 2.63 % w/w) and xylan (13.38 ± 0.49 % w/w). The total lipid content of the pretreated residue was 6.81% w/w, similar to that observed in untreated bagasse (6.30% w/w). Pretreatment improved the enzymatic digestibility of bagasse, allowing a recovery of 79% w/w and 86% w/w of glucose and xylose, respectively. The pretreatment and enzymatic saccharification resulted in a 2-fold increase in total lipid in enzymatic residue compared to the original bagasse. Furthermore, pretreatment and enzymatic hydrolysis enabled high sugar recovery while concentrating triglycerides and free fatty acids in the residue.

59 BASIC BIOLOGICAL SCIENCES↗

Enhancing Photosynthetic Production of Glycogen-Rich Biomass for Use as a Fermentation Feedstock

Current sources of fermentation feedstocks, i.e., corn, sugar cane, or plant biomass, fall short of demand for liquid transportation fuels and commodity chemicals in the United States. Aquatic phototrophs including cyanobacteria have the potential to supplement the supply of current fermentable feedstocks. In our strategy, cells are engineered to accumulate storage molecules including glycogen, cellulose, and/or lipid oils that can be extracted from harvested biomass and fed to heterotrophic organisms engineered to produce desired chemical products. Here in this manuscript, we examine the production of glycogen in the model cyanobacteria, Synechococcus sp. strain PCC 7002, and subsequent conversion of cyanobacterial biomass by an engineered Escherichia coli to octanoic acid as a model product. In effort to maximize glycogen production, we explored the deletion of catabolic enzymes and overexpression of GlgC, an enzyme that catalyzes the first committed step toward glycogen synthesis. We found that deletion of glgP increased final glycogen titers when cells were grown in diurnal light. Overexpression of GlgC led to a temporal increase in glycogen content but not in an overall increase in final titer or content. The best strains were grown, harvested, and used to formulate media for growth of E. coli. The cyanobacterial media was able to support the growth of an engineered E. coli and produce octanoic acid at the same titer as common laboratory media.

09 BIOMASS FUELS↗

Bioprocessing, Recovery, and Mass Balance of Vegetative Lipids from Metabolically Engineered “Oilcane” Demonstrates Its Potential as an Alternative Feedstock for Drop-In Fuel Production

Plant oils are increasingly in demand as renewable feedstocks for biodiesel and biochemicals. Currently, oilseeds are the primary source of plant oils. Although the vegetative tissues of plants express lipid metabolism pathways, they do not hyper- accumulate lipids. Elevated synthesis, storage, and accumulation of lipids in vegetative tissues have been achieved by metabolic engineering of sugarcane to produce “oilcane”. This study evaluates the potential of oilcane as a renewable feedstock for the co- production of lipids and fermentable sugars. Oilcane was grown under favorable climatic and field conditions in Florida (FLOC) as well as during an abbreviated growing season, outside its typical growing region, in Illinois (ILOC). The potential lipid yield of 0.35 tons/ha was projected from the hyperaccumulation of fatty acids in the stored vegetative biomass of FLOC, which is approaching the lipid yield of soybean (0.44 tons/ha). Processing of the vegetative tissues of oilcane recovered 0.20 tons/ha, which represents the recovery of 55% of the total lipids from FLOC. Chemical-free hydrothermal bioprocessing of ILOC and FLOC bagasse and leaves at 180 °C for 10 min prevented the degeneration of in situ plant lipids. Here, this allowed the recovery of lipids at the end of the bioprocess with a major fraction of lipids remaining in the biomass residues after pretreatment and saccharification. Improvements through refined biomass processing, crop management, and metabolic engineering are expected to boost lipid yields and make oilcane a prime feedstock for the production of biodiesel.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Growth-uncoupled propanediol production in a Thermoanaerobacterium thermosaccharolyticum strain engineered for high ethanol yield

Abstract Cocultures of engineered thermophilic bacteria can ferment lignocellulose without costly pretreatment or added enzymes, an ability that can be exploited for low cost biofuel production from renewable feedstocks. The hemicellulose-fermenting species Thermoanaerobacterium thermosaccharolyticum was engineered for high ethanol yield, but we found that the strains switched from growth-coupled production of ethanol to growth uncoupled production of acetate and 1,2-propanediol upon growth cessation, producing up to 6.7 g/L 1,2-propanediol from 60 g/L cellobiose. The unique capability of this species to make 1,2-propanediol from sugars was described decades ago, but the genes responsible were not identified. Here we deleted genes encoding methylglyoxal reductase, methylglyoxal synthase and glycerol dehydrogenase. Deletion of the latter two genes eliminated propanediol production. To understand how carbon flux is redirected in this species, we hypothesized that high ATP levels during growth cessation downregulate the activity of alcohol and aldehyde dehydrogenase activities. Measurements with cell free extracts show approximately twofold and tenfold inhibition of these activities by 10 mM ATP, supporting the hypothesized mechanism of metabolic redirection. This result may have implications for efforts to direct and maximize flux through alcohol dehydrogenase in other species.

09 BIOMASS FUELS↗

Co-Optimization of Fuels & Engines Findings and Impact (FY2015-FY2021 Report)

Breakthroughs from the U.S. Department of Energy Co-Optimization of Fuels & Engines (Co-Optima) initiative could make it possible to more rapidly cut emissions, reduce dependence on international petroleum, and contribute to ambitious national goals to slow global warming on the land, in the air, and across the water. Findings from the 6-year collaborative undertaking are now available in this report.

33 ADVANCED PROPULSION SYSTEMS↗