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Validation Assessment for the Soil Moisture Active Passive (SMAP) Level 4 Carbon (L4_C) Data Product Version 5

The post-launch Cal/Val phase of the SMAP mission is guided by two primary objectives for each science product team: 1) to calibrate, verify, and improve the performance of the science algorithms, and 2) validate accuracies of the science data products as specified in the SMAP Level-1 mission science requirements. Algorithm science and product maintenance activities during the SMAP extended mission phase have also involved periodic algorithm calibration and product refinements to maintain or enhance product consistency and performance as well as science utility. This report provides an assessment of the latest (Version 5) SMAP Level 4 Carbon (L4_C) product. The L4_C Version 5 (v5) global record now spans more than six years (March 2015 – present) of SMAP operations and has benefited from five major reprocessing updates to the operational product. These reprocessing events and L4_C product release updates have incorporated various algorithm refinements and calibration adjustments to account for similar refinements to the upstream GEOS land model assimilation system, SMAP brightness temperatures, and MODIS vegetation inputs used for L4_C processing. The SMAP L4_C algorithms utilize a terrestrial carbon flux model informed by daily surface and root zone soil moisture information contributed from the SMAP Level 4 Soil Moisture (L4_SM) product along with optical remote sensing-based (e.g. MODIS-based) land cover and canopy fractional photosynthetic active radiation (fPAR), and other ancillary biophysical data. The carbon flux model estimates global daily net ecosystem CO2 exchange (NEE) and the component carbon fluxes, namely, vegetation gross primary production (GPP) and soil heterotrophic respiration (Rh). Other L4_C product elements include surface (~0-5 cm depth) soil organic carbon (SOC) stocks and associated environmental constraints to these processes, including soil moisture-related controls on GPP and ecosystem respiration (Kimball et al. 2014, Jones et al. 2017). The L4_C product addresses SMAP carbon cycle science objectives by: 1) providing a direct link between terrestrial carbon fluxes and underlying freeze/thaw and soil moisture-related constraints to these processes, 2) documenting primary connections between terrestrial water, energy and carbon cycles, and 3) improving understanding of terrestrial carbon sink activity. The SMAP L4_C algorithms and operational product are mature and at a CEOS Validation Stage 4 level (Jackson et al. 2012) based on extensive validation of the multi-year record against a diverse array of independent benchmarks, well characterized global performance, and systematic refinements gained from five major reprocessing events. There are no Level-1 mission science requirements for the L4_C product; however, self-imposed requirements have been established focusing on NEE as the primary product field for validation, and on demonstrating L4_C accuracy and success in meeting product science requirements (Jackson et al. 2012). The other L4_C product fields also have strong utility for carbon science applications (e.g., Liu et al. 2019, Endsley et al. 2020); however, analysis of these other fields is considered secondary relative to primary validation activities focusing on NEE. The L4_C targeted accuracy requirements are to meet or exceed a mean unbiased root-mean-square error (ubRMSE, or standard deviation of the error) for NEE of 1.6 g C m-2 d-1 and 30 g C m-2 yr-1, emphasizing northern (≥45°N) boreal and arctic ecosystems; this accuracy is similar to that of tower eddy covariance measurement-based observations (Baldocchi 2008). Methods used for the latest v5 L4_C product performance and validation assessment have been established from the SMAP Cal/Val plan and previous studies (Jackson et al. 2012, Jones et al. 2017) and include: 1) consistency evaluations of the product fields against earlier product releases (version 4 or earlier); 2) comparisons of daily carbon flux estimates with independent tower eddy 4covariance measurement-based daily carbon (CO2) flux observations from core tower validation sites (CVS); and 3) consistency checks against other global carbon products, including soil carbon inventory records, global GPP records derived from tower observation upscaling methods, and satellite-based observations of canopy solar induced chlorophyll fluorescence (SIF) as a surrogate for GPP. Metrics used to evaluate relative agreement between L4_C product fields and observational benchmarks include correlation (r-value), RMSE differences, bias and model sensitivity diagnostics. Following these validation criteria, the present report provides a validation assessment of the latest L4_C product release (v5). Detailed descriptions of the L4_C algorithm and additional global product accuracy and performance results are given elsewhere (Jones et al. 2017, Endsley et al. 2020). The v5 L4_C product replaces earlier product versions and continues to show: (i) accuracy and performance levels meeting or exceeding SMAP L4_C science requirements; (ii) improvement over the previous product version (version 4); and (iii) suitability for a diversity of science applications. Example L4_C applications from the recent literature include clarifying environmental trends and controls on the northern terrestrial carbon sink (Liu et al. 2019), diagnosing drought-related impacts on ecosystem productivity (Li et al. 2020), and regional monitoring of cropland conditions for projecting annual yields (Wurster et al. 2020). 2EXPECTED L4_C ALGORITHM AND PRODUCTPERFORMANCE The L4_C algorithm performance, including variance and uncertainty estimates of model outputs, was determined during the mission pre-launch phase through spatially explicit model sensitivity studies using available model inputs similar to those currently being used for operational production and evaluating the resulting model simulations over the observed range of northern (≥45 °N) and global conditions (Kimball et al. 2012, Entekhabi et al. 2014). The L4_C algorithm options were also evaluated during the mission prelaunch phase, including deriving canopy fPAR from lower order NDVI (Normalized Difference Vegetation Index) inputs in lieu of using MODIS (MOD15) fPAR; and including an explicit model representation of boreal fire disturbance recovery impacts. These results indicated that the L4_C accuracy requirements (i.e., NEE ubRMSE ≤ 30 g C m-2 yr-1or ≤ 1.6 g C m-2 d-1) could be met from the baseline algorithms over more than 82% and 89% of global and northern vegetated land areas, respectively (Yi et al. 2013, Kimball et al. 2014). The global L4_C algorithm error budget for NEE derived during the mission prelaunch phase indicated that the estimated NEE ubRMSE uncertainty is proportional to GPP and is therefore larger in higher biomass productivity areas, including forests and croplands (Kimball et al. 2014). Likewise, NEE ubRMSE uncertainty is expected to be lower in less-productive areas, including grasslands and shrublands. Expected model NEE ubRMSE levels were also generally within targeted accuracy levels for characteristically less-productive boreal and Arctic biomes, even though relative model error as a proportion of total productivity (NEE RMSE / GPP) may be large in these areas. The estimated NEE uncertainty was lower than expected in some warmer tropical high biomass productivity areas (e.g. Amazon rainforest) because of reduced low temperature and moisture constraints to the L4_C respiration calculations so that the bulk of model uncertainty is contributed by GPP in these areas. Model NEE uncertainty in the African Congo was estimated to

SMAP↗

Decision Support Tool Evaluation Report for Coral Reef Early Warning System (CREWS) Version 7.0

The Coral Reef Early Warning System (CREWS) is operated by NOAA's Office of Oceanic and Atmospheric Research as part of its Coral Reef Watch program in response to the deteriorating global state of coral reef and related benthic ecosystems. In addition to sea surface temperatures (SSTs), the two most important parameters used by the CREWS network in generating coral reef bleaching alerts are 1) wind speed and direction and 2) photosynthetically available radiation (PAR). NASA remote sensing products that can enhance CREWS in these areas include SST and PAR products from the Moderate Resolution Imaging Spectroradiometer (MODIS) and wind data from the Quick Scatterometer (QuikSCAT). CREWS researchers are also interested in chlorophyll, chromophoric dissolved organic matter (CDOM), and salinity. Chlorophyll and CDOM are directly available as NASA products, while rainfall (an available NASA product) can be used as a proxy for salinity. Other potential NASA inputs include surface reflectance products from MODIS, the Advanced Spaceborne Thermal Emission and Reflection Radiometer, and Landsat. This report also identifies NASA-supported ocean circulation models and products from future satellite missions that might enchance the CREWS DST.

D'Sa, Eurico↗

Similar photosynthetic but different yield responses of C 3 and C 4 crops to elevated O 3

The deleterious effects of ozone (O 3 ) pollution on crop physiology, yield, and productivity are widely acknowledged. It has also been assumed that C 4 crops with a carbon concentrating mechanism and greater water use efficiency are less sensitive to O 3 pollution than C 3 crops. This assumption has not been widely tested. Therefore, we compiled 46 journal articles and unpublished datasets that reported leaf photosynthetic and biochemical traits, plant biomass, and yield in five C 3 crops (chickpea, rice, snap bean, soybean, and wheat) and four C 4 crops (sorghum, maize, Miscanthus × giganteus , and switchgrass) grown under ambient and elevated O 3 concentration ([O 3 ]) in the field at free-air O 3 concentration enrichment (O 3 -FACE) facilities over the past 20 y. When normalized by O 3 exposure, C 3 and C 4 crops showed a similar response of leaf photosynthesis, but the reduction in chlorophyll content, fluorescence, and yield was greater in C 3 crops compared with C 4 crops. Additionally, inbred and hybrid lines of rice and maize showed different sensitivities to O 3 exposure. This study quantitatively demonstrates that C 4 crops respond less to elevated [O 3 ] than C 3 crops. This understanding could help maintain cropland productivity in an increasingly polluted atmosphere.

60 APPLIED LIFE SCIENCES↗

Evaluation of ERTS MSS digital data for monitoring water in the lower Chesapeake Bay area

Water samples collected during the ERTS overpasses of the lower Chesapeake Bay area have been analyzed for chlorophyll, particles, and sediment. Five ERTS images were sufficiently cloud free that a correlation analysis of radiance values from the MSS bands with the water parameters could be performed. A low correlation was established for chlorophyll, except during algal blooms, when band 6 was responsive. There was a fair to good correlation with particles for combinations of band 5, particularly bands (5 minus 6). Sediment correlations were excellent for band 5 or combinations of band 5 with bands 4 and 6. It was evident that such factors as the atmosphere, tide, and different water masses were tending to confuse the data.

Bowker, D. E.↗

Evaluation of the Harmful Algal Bloom Mapping System (HABMapS) and Bulletin

The National Oceanic and Atmospheric Administration (NOAA) Harmful Algal Bloom (HAB) Mapping System and Bulletin provide a Web-based geographic information system (GIS) and an e-mail alert system that allow the detection, monitoring, and tracking of HABs in the Gulf of Mexico. NASA Earth Science data that potentially support HABMapS/Bulletin requirements include ocean color, sea surface temperature (SST), salinity, wind fields, precipitation, water surface elevation, and ocean currents. Modeling contributions include ocean circulation, wave/currents, along-shore current regimes, and chlorophyll modeling (coupled to imagery). The most immediately useful NASA contributions appear to be the 1-km Moderate Resolution Imaging Spectrometer (MODIS) chlorophyll and SST products and the (presently used) SeaWinds wind vector data. MODIS pigment concentration and SST data are sufficiently mature to replace imagery currently used in NOAA HAB applications. The large file size of MODIS data is an impediment to NOAA use and modified processing schemes would aid in NOAA adoption of these products for operational HAB forecasting.

Hall, Callie↗

Use of ERTS imagery in air pollution and marine biology studies, tasks 1 through 3

There are no author-identified significant results in this report. The general suitability of ERTS imagery in detecting ground originated air pollution has proved to be excellent. The quality and resolution exceeded expectations and has permitted in some instances location of point sources to within a thousand feet. Suitable techniques have not yet been developed for determining or measuring area and line sources of air pollution. A major problem has been cloud cover that has persisted over the area of primary interest, the Chesapeake Bay. Work has been completed on mounting the shipboard transmissometer which will be used for investigations to relate the chlorophyll and suspended sediment content in the waters of the Lower Chesapeake Bay to ERTS-1 imagery. Water sampling, plankton analysis, and preparations for sea collection of water truth along the eastern continental shelf of the U.S. have been completed for use in comparisons with ERTS-1 data.

Copeland, G. E.↗

Phenotyping stomatal closure by thermal imaging for GWAS and TWAS of water use efficiency-related genes

Abstract Stomata allow CO2 uptake by leaves for photosynthetic assimilation at the cost of water vapor loss to the atmosphere. The opening and closing of stomata in response to fluctuations in light intensity regulate CO2 and water fluxes and are essential for maintaining water-use efficiency (WUE). However, a little is known about the genetic basis for natural variation in stomatal movement, especially in C4 crops. This is partly because the stomatal response to a change in light intensity is difficult to measure at the scale required for association studies. Here, we used high-throughput thermal imaging to bypass the phenotyping bottleneck and assess 10 traits describing stomatal conductance (gs) before, during and after a stepwise decrease in light intensity for a diversity panel of 659 sorghum (Sorghum bicolor) accessions. Results from thermal imaging significantly correlated with photosynthetic gas exchange measurements. gs traits varied substantially across the population and were moderately heritable (h2 up to 0.72). An integrated genome-wide and transcriptome-wide association study identified candidate genes putatively driving variation in stomatal conductance traits. Of the 239 unique candidate genes identified with the greatest confidence, 77 were putative orthologs of Arabidopsis (Arabidopsis thaliana) genes related to functions implicated in WUE, including stomatal opening/closing (24 genes), stomatal/epidermal cell development (35 genes), leaf/vasculature development (12 genes), or chlorophyll metabolism/photosynthesis (8 genes). These findings demonstrate an approach to finding genotype-to-phenotype relationships for a challenging trait as well as candidate genes for further investigation of the genetic basis of WUE in a model C4 grass for bioenergy, food, and forage production.

54 ENVIRONMENTAL SCIENCES↗

Assessment of AVIRIS data from vegetated sites in the Owens Valley, California

Airborne Visible/Infrared Imaging Spectrometer (AVIRIS) data were acquired from the Bishop, CA area, located at the northern end of the Owens Valley, on July 30, 1987. Radiometrically-corrected AVIRIS data were flat-field corrected, and spectral curves produced and analyzed for pixels taken from both native and cultivated vegetation sites, using the JPS SPAM software program and PC-based spreadsheet programs. Analyses focussed on the chlorophyll well and red edge portions of the spectral curves. Results include the following: AVIRIS spectral data are acquired at sufficient spectral resolution to allow detection of blue shifts of both the chlorophyll well and red edge in moisture-stressed vegetation when compared with non-stressed vegetation; a normalization of selected parameters (chlorophyll well and near infrared shoulder) may be used to emphasize the shift in red edge position; and the presence of the red edge in AVIRIS spectral curves may be useful in detecting small amounts (20 to 30 pct cover) of semi-arid and arid vegetation ground cover. A discussion of possible causes of AVIRIS red edge shifts in respsonse to stress is presented.

Rock, B. N.↗

Spectral characteristics of normal and nutrient-deficient maize leaves

Reflectance, transmittance and absorbance spectra of normal and six types of mineral-deficient (N,P,K,S,Mg and Ca) maize (Zea mays L.) leaves were analyzed at 30 selected wavelengths along the electromagnetic spectrum from 500 to 2600 nm. Chlorophyll content and percent leaf moisture were also determined. Leaf thermograms were obtained for normal, N- and S- deficient leaves. The results of the analysis of variance showed significant differences in reflectance, transmittance and absorbance in the visible wavelengths among leaf numbers 3, 4, and 5, among the seven nutrient treatments, and among the interactions of leaves and treatments. In the reflective infrared wavelengths only treatments produced significant differences. The chlorophyll content of leaves was reduced in all deficiencies in comparison to controls. Percent moisture was increased in S-, Mg- and N- deficiencies. Positive correlation (r = 0.707) between moisture content and percent absorption at both 1450 and 1930 nm were obtained. Polynomial regression analysis of leaf thickness and leaf moisture content showed that these two variables were significantly and directly related (r = 0.894).

Al-Abbas, A. H.↗

The feasibility of utilizing remotely sensed data to assess and monitor oceanic gamefish

An investigation was conducted to establish the feasibility of utilizing remotely sensed data acquired from aircraft and satellite platforms to provide information concerning the distribution and abundance of oceanic gamefish. The data from the test area was jointly acquired by NASA, the Navy, the Air Force and NOAA/NMFS elements and private and professional fishermen in the northeastern Gulf of Mexico. The data collected has made it possible to identify fisheries significant environmental parameters for white marlin. Prediction models, based on catch data and surface truth information, were developed and demonstrated a potential for significantly reducing search by identifying areas that have a high probability of productivity. Three of the parameters utilized by the models, chlorophyll-a, sea surface temperature, and turbidity were inferred from aircraft sensor data and were tested. Effective use of Skylab data was inhibited by cloud cover and delayed delivery. Initial efforts toward establishing the feasibility of utilizing remotely sensed data to assess and monitor the distribution of oceanic gamefish has successfully identified fisheries significant oceanographic parameters and demonstrated the capability of remotely measuring most of the parameters.

Savastano, K. J.↗

Effects of heavy metals on the absorbance and reflectance spectra of plants

The spectral responses of plants to various concentrations of heavy metals in their rooting media are investigated in relation to the application of remote sensing methods to the detection of vegetation under stress. Absorption photometry of chloroplasts, measurements of metal and chlorophyll concentrations and reflectance spectrometry were performed on leaves of pea, sunflower and soybean plants grown under greenhouse conditions with the addition of various concentrations of Cd, Cu, Pb and Zn to their rooting media and on leaves of oak trees growing naturally in an area of a copper-arsenic mineralization. Under laboratory conditions, the most general effect observed was growth inhibition and ultimately death, with pea plants also exhibiting changes of chlorophyll a/ chlorophyll b ratios with Cd and Cu and reflectance increases in the visible and decreases in the infrared. Although results for other species indicate that reflectance effects are dependent on species, correlations between reflectance and metal exposure is confirmed by the field investigations. It is concluded that a remote sensing system would be improved by the inclusion of bands around 1.65 and 2.20 microns to detect soil mineralization from plant spectra.

Horler, D. N. H.↗

Prediction of leaf chemistry by the use of visible and near infrared reflectance spectroscopy

The chemical content of dry, ground leaf material sampled from deciduous and conifer tree species from sites in Alaska, Wisconsin, and California was estimated using visible and shortwave IR spectroscopy. Seven chemical components - sugar, starch, protein, cellulose, total chlorophyll, lignin, and total nitrogen - were analyzed by wet chemical methods and their concentrations regressed against log 1/rho and first and second differences of log 1/rho (where rho is measured reflectance) at wavelengths selected by stepwise regression. Predictions of chemical concentrations based on cross validation suggest that this technique may be useful for extracting vegetation canopy biochemical information by remote sensing.

Card, Don H.↗

Chlorophyll to zeaxanthin energy transfer in nonphotochemical quenching: An exciton annihilation-free transient absorption study

Zeaxanthin (Zea) is a key component in the energy-dependent, rapidly reversible, nonphotochemical quenching process (qE) that regulates photosynthetic light harvesting. Previous transient absorption (TA) studies suggested that Zea can participate in direct quenching via chlorophyll (Chl) to Zea energy transfer. However, the contamination of intrinsic exciton–exciton annihilation (EEA) makes the assignment of TA signal ambiguous. In this study, we present EEA-free TA data using Nicotiana benthamiana thylakoid membranes, including the wild type and three NPQ mutants (npq1, npq4, and lut2) generated by CRISPR/Cas9 mutagenesis. The results show a strong correlation between excitation energy transfer from excited Chl Q y to Zea S 1 and the xanthophyll cycle during qE activation. Notably, a Lut S 1 signal is absent in the npq1 thylakoids which lack zeaxanthin. Additionally, the fifth-order response analysis shows a reduction in the exciton diffusion length (L D ) from 62 ± 6 nm to 43 ± 3 nm under high light illumination, consistent with the reduced range of exciton motion being a key aspect of plants’ response to excess light.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Chiral Biomarkers and Microfossils in Carbonaceous Meteorites

Homochirality of the biomolecules (D-sugars of DNA and RNA and L-amino acids of proteins) is a fundamental property of all life on Earth. Abiotic mechanisms yield racemic mixtures (D/L=1) of chiral molecules and after the death of an organism, the enantiopure chiral biomolecules slowly racemize. Several independent investigators have now established that the amino acids present in CI1 and CM2 carbonaceous meteorites have a moderate to strong excess of the L-enantiomer. Stable isotope data have established that these amino acids are both indigenous and extraterrestrial. Carbonaceous meteorites also contain many other strong chemical biomarkers including purines and pyrimidines (nitrogen heterocycles of nucleic acids); pristine and phytane (components of the chlorophyll pigment) and morphological biomarkers (microfossils of filamentous cyanobacteria). Energy dispersive X-ray Spectroscopy (EDS) analysis reveals that nitrogen is below the detectability level in most of the meteorite filaments as well as in Cambrian Trilobites and filaments of 2.7 Gya Archaean cyanobacteria from Karelia. The deficiency of nitrogen in the filaments and the total absence of sugars, of twelve of the life-critical protein amino acids, and two of the nucleobases of DNA and RNA provide clear and convincing evidence that these filaments are not modern biological contaminants. This paper reviews the chiral, chemical biomarkers morphological biomarkers and microfossils in carbonaceous meteorites. This paper reviews chiral and morphological biomarkers and discusses the missing nitrogen, sugars, protein amino acids, and nucleobases as ?bio-discriminators? that exclude modern biological contaminants as a possible explanation for the permineralized cyanobacterial filaments found in the meteorites.

Hoover, Richard B.↗

A model for the origin of photosynthesis--III. The ultraviolet photochemistry of uroporphyrinogen

The photochemical ramifications of the high ultraviolet flux on the primordial earth prior to the formation of the ozone layer have been considered in a study of the ultraviolet photochemistry of uroporphyrinogen (urohexahydroporphyrin), a colorless compound which absorbs strongly at wavelengths less than 220 nanometers. Urohexahydroporphyrin was investigated since it is the first macrocycle formed on the biosynthetic pathway of chlorophyll and can be used to test the hypothesis that the biosynthetic pathway to chlorophyll recapitulates the evolutionary history of photosynthesis. When urohexahydroporphyrin is illuminated in aqueous anaerobic solution, hydrogen gas is produced. More hydrogen gas is produced in the presence of a colloidal platinum catalyst. The products of the photooxidation of urohexahydroporphyrin are urotetrahydroporphyrin (uroporphomethene) and uroporphyrin. This research shows how the oxidation of uroporphyrinogen to uroporphyrin, the first biogenetic porphyrin, could have occurred anaerobically and abiotically on the primordial earth.

Evolution, Chemical↗

Asymmetry in the Rate of Warming and the Phenology of Seasonal Blooms in the Northeast US Shelf Ecosystem

Predicting the impact of marine ecosystem warming on the timing and magnitude of phytoplankton production is challenging. For example, warming can advance the progression of stratification thereby changing the availability of nutrients to surface phytoplankton, or influence the surface mixed layer depth, thus affecting light availability. Here, we use a time series of sea surface temperature (SST) and chlorophyll remote sensing products to characterize the response of the phytoplankton community to increased temperature in the Northeast US Shelf Ecosystem. The rate of change in SST was higher in the summer than in winter in all ecoregions resulting in little change in the timing and magnitude of the spring thermal transition compared to a significant change in the autumn transition. Along with little phenological shift in spring thermal conditions, there was also no evidence of a change in spring bloom timing and duration. However, we observed a change in autumn bloom timing in the Georges Bank ecoregion, where bloom initiation has shifted from late September to late October between 1998 and 2020—on average 33 d later. Bloom duration in this ecoregion also shortened from ∼7.5 to 5 weeks. The shortened autumn bloom may be caused by later overturn in stratification known to initiate autumn blooms in the region, whereas the timing of light limitation at the end of the bloom remains unchanged. These changes in bloom timing and duration appear to be related to the change in autumn thermal conditions and the significant shift in autumn thermal transition. These results suggest that the spring bloom phenology in this temperate continental shelf ecosystem may be more resilient to thermal climate change effects than blooms occurring in other times of the year.

climate change↗

Satellite detection of phytoplankton export from the mid-Atlantic Bight during the 1979 spring bloom

Analysis of Coastal Zone Color Scanner (CZCS) imagery confirms shipboard and in situ moored fluorometer observations of resuspension of near-bottom chlorophyll within surface waters (1 to 10 m) by northwesterly wind events in the mid-Atlantic Bight. As much as 8 to 16 micrograms chl/l are found during these wind events from March to May, with a seasonal increase of algal biomass until onset of stratification of the water column. Rapid sinking or downwelling apparently occurs after subsequent wind events, however, such that the predominant surface chlorophyll pattern is approx. 0.5 to 1.5 micrograms/l over the continental shelf during most of the spring bloom. Perhaps half of the chlorophyll increase observed by satellite during a wind resuspension event represents in-situ production during the 4 to 5 day interval, with the remainder attributed to accumulation of algal biomass previously produced and temporarily stored within near-bottom water. Present calculations suggest that about 10% of the primary production of the spring bloom may be exported as ungrazed phytoplankton carbon from mid-Atlantic shelf waters to those of the continental slope.

Walsh, J. J.↗

Interpretation of Observations of Trans-Spectral Phenomena Acquired Using Hyperspectral Sensors Aboard a Remotely Operated Vehicle in Exuma Sound

Hyper-spectral (512-channel) optical data acquired during a relatively deep (102m) dive of our ROSEBUD Remotely Operated Vehicle (ROV) in the clear waters of Exuma Sound, Bahamas provided the opportunity to investigate the trans-spectral shift of photonic energy (inelastic scattering) as a function of water depth. Results show a convolution of several spectral processes (e.g. absorption, scattering) involving water molecules, dissolved material and particulates as well as trans-spectral (inelastic) processes involving fluorescence by water molecules (Raman), dissolved material and chlorophyll. The spectral signatures of these convolved causes and effects allow deconvolution with a hyperspectral approach. Intrinsic to the convolution was the ability to position the vehicle at depths where Raman fluorescence dominated at red wavelengths. Results show that the calculated Raman absorption coefficients are generally consistent with historical values (i.e. 0.9 x 10(sup)-4 at 525 nm excitation) and that an angstrom exponent of 5 is more appropriate than the often cited value of 4.

Costello, D.↗