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At least 1,711 records · Page 95
All sourcesNASA NTRSDOE OSTI
DOE OSTI2023

Application of prophage sequence analysis to investigate a disease outbreak involving Salmonella Adjame, a rare serovar and implications for the population structure

Introduction Outbreak investigation of foodborne salmonellosis is hindered when the food source is contaminated by multiple strains of Salmonella , creating difficulties matching an incriminated organism recovered from patients with the specific strain in the suspect food. An outbreak of the rare Salmonella Adjame was caused by multiple strains of the organism as revealed by single-nucleotide polymorphism (SNP) variation. The use of highly discriminatory prophage analysis to characterize strains of Salmonella should enable a more precise strain characterization and aid the investigation of foodborne salmonellosis. Methods We have carried out genomic analysis of S. Adjame strains recovered during the course of a recent outbreak and compared them with other strains of the organism ( n = 38 strains), using SNPs to evaluate strain differences present in the core genome, and prophage sequence typing (PST) to evaluate the accessory genome. Phylogenetic analyses were performed using both total prophage content and conserved prophages. Results The PST analysis of the S. Adjame isolates showed a high degree of strain heterogeneity. We observed small clusters made up of 2-6 isolates ( n = 27) and singletons ( n = 11) in stark contrast with the three clusters observed by SNP analysis. In total, we detected 24 prophages of which only four were highly prevalent, namely: Entero_p88 (36/38 strains), Salmon_SEN34 (35/38 strains), Burkho_phiE255 (33/38 strains) and Edward_GF (28/38 strains). Despite the marked strain diversity seen with prophage analysis, the distribution of the four most common prophages matched the clustering observed using core genome. Discussion Mutations in the core and accessory genomes of S. Adjame have shed light on the evolutionary relationships among the Adjame strains and demonstrated a convergence of the variations observed in both fractions of the genome. We conclude that core and accessory genomes analyses should be adopted in foodborne bacteria outbreak investigations to provide a more accurate strain description and facilitate reliable matching of isolates from patients and incriminated food sources. The outcomes should translate to a better understanding of the microbial population structure and an 46 improved source attribution in foodborne illnesses.

Gao, Ruimin↗
NASA NTRS1991

Exploration of relationships between low-Ti and high-Ti pristine lunar glasses using an armalcolite assimilation model

The pristine glasses of Delano are the most primitive lunar basaltic magma compositions discovered to date. They are grouped into two (and possibly three) arrays: a low-alumina array and a high alumina array. These glasses are very olivine normative and are multiply saturated at pressures of approximately 20 kbar, implying a depth of origin of 400 to 500 km in the Moon. Thus, these glasses appear to be the best candidates for primitive partial melts of the upper lunar mantle. One of the most perplexing characteristics of the pristine glasses is a positive correlation between Ni and SiO2 within each array. This is contrary to the terrestrial experience, where Ni is observed to positively correlate with MgO and negatively correlate with SiO2. These systematics are believed to be due to the depletion of Ni by olivine fractionation. The difference between the lunar and terrestrial Ni vs. SiO2 trends may be partially ascribed to the Ti-rich component. In the case of the pristine glasses, SiO2 increases not because of olivine fractionation, but because they contain less of the high-Ti component. An attempt was made to model this variation in Ni and SiO2 with a simple assimilation-fractional crystallization (AFC) model. Silica and Ni both decreased dramatically as the AFC process proceeded. Only 15 to 20 percent AFC was necessary to produce the observed variation, and the SiO2 vs. Ni variation was modeled quite well. The D(Ni) for olivine/liquid in this model was taken to be 10 and the olivine was assumed to be Fe sub 80. However, the results of this model for Ti and Mg were less than satisfactory. It seemed difficult to achieve the high TiO2 contents of some glasses (16 to 17 wt. percent) by this method. Continual addition of ilmenite by AFC could indeed raise the titania concentrations to the necessary levels, but only by enriching the magma in FeO and greatly depleting the magma in MgO. An attempt was made to circumvent this problem by using armalcolite, (Fe, Mg)Ti2O5, in the AFC model, and the results are presented.

Jones, John H.↗
NASA NTRS2018

Multiwavelength Light Curves of Two Remarkable Sagittarius A* Flares

Sgr A* , the supermassive black hole (SMBH) at the center of our Milky Way Galaxy, is known to be a variable source of X-ray, near-infrared (NIR), and submillimeter radiation and therefore a prime candidate to study the electromagnetic radiation generated by mass accretion flow onto a black hole and/or a related jet. Disentangling the power source and emission mechanisms of this variability is a central challenge to our understanding of accretion flows around SMBHs. Simultaneous multiwavelength observations of the flux variations and their time correlations can play an important role in obtaining a better understanding of possible emission mechanisms and their origin. This paper presents observations of two flares that both apparently violate the previously established patterns in the relative timing of submillimeter/NIR/X-ray flares from Sgr A* . One of these events provides the first evidence of coeval structure between NIR and submillimeter flux increases, while the second event is the first example of the sequence of submillimeter/X-ray/NIR flux increases all occurring within ∼1 hr. Each of these two events appears to upend assumptions that have been the basis of some analytic models of flaring in Sgr A* . However, it cannot be ruled out that these events, even though unusual, were just coincidental. These observations demonstrate that we do not fully understand the origin of the multiwavelength variability of Sgr A* and show that there is a continued and important need for long-term, coordinated, and precise multiwavelength observations of Sgr A* to characterize the full range of variability behavior

accretion↗
NASA NTRS1995

Directivity and its energy dependence in solar flare energetic emission

We have studied 72 solar flares simultaneously observed by the Gamma-Ray Spectrometer (GRS, 0.3-1 MeV) and the Hard X-Ray Burst Spectrometer (HXRBS, 30-500 keV) on the Solar Maximum Mission (SMM). Using the spectral analysis results, we studied spectal and size distribution center-to-limb variations for both instruments. The GRS observations show significant center-to-limb variations in both spectral and size distributions, while HXRBS observations show insignificant variations. In general, the GRS spectra are harder than the HXRBS spectra, and their difference increases from center to limb, suggesting that a flattening of the spectrum above 300 keV is inevitable for the gamma-ray emissions. We corrected for the effect of spacecraft pointing and combined HXRBS and GRS data to obtain spectra over the energy range of 0.03-1 MeV. The fluences at various energies were calculated and normalized to the total fluence of the burst to measure the directivity. It is found that the directivity increases with increasing energy, from 1.1 at 50 keV to 6.5 at 1 MeV. These results are consistent with anisotropic electron beams and the physics of Coulomb collision and bremsstrahlung.

Li, Peng↗
NASA NTRS2003

Precision Clocks in Space and alpha-Variations

Important developments of both in theoretical and observational cosmology have fueled considerable interest in searches for variations of the fine structure constant. Experimentally, Webb et. al. have found evidence for a cosmological variation of the fine structure constant through an analysis of the absorption lines in galactic halos from quasar-emitted light. Recently developed small ion atomic clocks enable Solar System tests for equivalence principle (EP) violating (alpha)-variations by way of rate-comparisons of three ultra-stable atomic clocks near-to and far-from the sun where gravitational red-shift changes are more than 10(exp 4) larger than in low Earth orbit. No space tests of the EP have been made in nearly 30 years, since the GP-A hydrogen maser reached a 10,000 km apogee confirming EP red-shift predictions ~1 part in 10(exp 4). Today's small ion clocks, nearly 100x more stable and 100x smaller then the GP-A H-maser, could probe for EP violating scalar fields near the sun for mission costs comparable to low Earth orbiters and improve the GP-A sensitivity by 5 to 6 orders of magnitude.

(alpha)-variation↗
DOE OSTI2017

Discovery of Scattering Polarization in the Hydrogen Ly α Line of the Solar Disk Radiation

There is a thin transition region (TR) in the solar atmosphere where the temperature rises from 10,000 K in the chromosphere to millions of degrees in the corona. Little is known about the mechanisms that dominate this enigmatic region other than the magnetic field plays a key role. The magnetism of the TR can only be detected by polarimetric measurements of a few ultraviolet (UV) spectral lines, the Ly α line of neutral hydrogen at 121.6 nm (the strongest line of the solar UV spectrum) being of particular interest given its sensitivity to the Hanle effect (the magnetic-field-induced modification of the scattering line polarization). We report the discovery of linear polarization produced by scattering processes in the Ly α line, obtained with the Chromospheric Lyman-Alpha Spectro-Polarimeter (CLASP) rocket experiment. The Stokes profiles observed by CLASP in quiet regions of the solar disk show that the Q / I and U / I linear polarization signals are of the order of 0.1% in the line core and up to a few percent in the nearby wings, and that both have conspicuous spatial variations with scales of ∼10 arcsec. These observations help constrain theoretical models of the chromosphere–corona TR and extrapolations of the magnetic field from photospheric magnetograms. In fact, the observed spatial variation from disk to limb of polarization at the line core and wings already challenge the predictions from three-dimensional magnetohydrodynamical models of the upper solar chromosphere.

79 ASTRONOMY AND ASTROPHYSICS↗
NASA NTRS1991

Calibration of EOS multispectral imaging sensors and solar irradiance variability

Earth Observation System (EOS) optical multispectral imaging sensors provide images of the earth at various spectral and spatial resolutions, in the visible (VIS) and infrared (IR) regions of the solar spectrum. Accurate knowledge of extraterrestrial solar spectral irradiance and its variations with time, are needed to trace sensor calibration in space, and for the development of terrestrial atmospheric models needed in data validation. A brief review of the extraterrestrial solar VIS/IR spectral irradiance available in the literature will be reviewed, and the need to develop an extraterrestrial solar spectral irradiance for the EOS studies will be pointed out. The solar calibration of the Earth Radiation Budget Experiments (ERBE), earth-viewing sensors will be discussed. Observed variations in the solar constant (solar irradiance, at the mean sun-earth distance of one astronomical unit, integrated over all wavelengths), and solar spectral irradiance with solar activity and its implications for EOS studies also will be discussed.

Mecherikunnel, Ann↗
DOE OSTI2023

Genomic variation across Chinook salmon populations reveals effects of a duplication on migration alleles and supports fine scale structure

Abstract The distribution of ecotypic variation in natural populations is influenced by neutral and adaptive evolutionary forces that are challenging to disentangle. This study provides a high‐resolution portrait of genomic variation in Chinook salmon ( Oncorhynchus tshawytscha ) with emphasis on a region of major effect for ecotypic variation in migration timing. With a filtered data set of ~13 million single nucleotide polymorphisms (SNPs) from low‐coverage whole genome resequencing of 53 populations (3566 barcoded individuals), we contrasted patterns of genomic structure within and among major lineages and examined the extent of a selective sweep at a major effect region underlying migration timing (GREB1L/ROCK1). Neutral variation provided support for fine‐scale structure of populations, while allele frequency variation in GREB1L/ROCK1 was highly correlated with mean return timing for early and late migrating populations within each of the lineages ( r 2 = .58–.95; p < .001). However, the extent of selection within the genomic region controlling migration timing was much narrower in one lineage (interior stream‐type) compared to the other two major lineages, which corresponded to the breadth of phenotypic variation in migration timing observed among lineages. Evidence of a duplicated block within GREB1L/ROCK1 may be responsible for reduced recombination in this portion of the genome and contributes to phenotypic variation within and across lineages. Lastly, SNP positions across GREB1L/ROCK1 were assessed for their utility in discriminating migration timing among lineages, and we recommend multiple markers nearest the duplication to provide highest accuracy in conservation applications such as those that aim to protect early migrating Chinook salmon. These results highlight the need to investigate variation throughout the genome and the effects of structural variants on ecologically relevant phenotypic variation in natural species.

Horn, Rebekah L.↗
NASA NTRS1994

EUVAC: A solar EUV flux model for aeronomic calculations

This paper presents a new solar Extreme Ultraviolet (EUV) flux model for aeronomic calulations (EUVAC), which is based on the measured F74113 solar EUV reference spectrum. The model provides fluxes in the 37 wavelength bins that are in widespread use. This paper also presents cross sections to be used with the EUVAC flux model to calculate photoionization rates. The flux scaling for solar activity is accomplished using a proxy-based on the F10.7 index and its 81-day average together with the measured solar flux variation from the EUVS instrument on the Atmosphere Explorer E satellite. This new model produces 50-575 A integrated EUV fluxes in good agreement with rocket observations. The solar cycle variation of the chromospheric fluxes agrees well with the measured variation of the Lyman alpha flux between 1982 and 1988. In addition, the theoretical photoelectron fluxes, calculated using the new EUV flux model, are in good agreement with the solar minimum photoelectron fluxes from the Atmosphere Explorer E satellite and also with the solar maximum photoelectron fluxes from the Dynamics Explorer satellite. Its relative simplicity coupled with its ability to reproduce the 50-575 A solar EUV flux as well as the measured photoelectron spectrum makes the model well suited for aeronomic applications. However, EUVAC is not designed to accurately predict the solar flux variability for numerous individual lines.

Richards, P. G.↗
NASA NTRS1982

Periodic spectrum variations in helium-rich stars

Spectroscopic observations of four helium-rich stars are presented. In HD 37776, antiphase variations of Si III and He I have been found, which represent another point of similarity to the Ap phenomenon. The remarkable H-alpha emission variations in Sigma Ori E are illustrated with uniform phase coverage, and strict periodicity over a five-year interval is shown. A radial-velocity study of HD 64740 shows constancy to within the accuracy of the observations. Finally, Delta Ori B is confirmed as a helium-rich star.

Walborn, N. R.↗
NASA NTRSDate not supplied

Perturbations, TTVs & the (Un)reliability of Ephemerides of Kepler Planets

Fractional uncertainties quoted for orbital periods of the vast majority of planet candidates listed in recent Kepler catalogs are $<10^{-5}$, with values $\sim 10^{-6}$ (corresponding to 2 minutes per 4 years) being typical. These small uncertainties suggest ephemeris predictions for most Kepler planets are robust for decades to come. Tabulated periods list formal uncertainties in the mean times between midpoints of successive transits during the time interval in which transits were observed. However, transit timing variations (TTVs) produce errors in estimates of some planets’ orbital periods that need to be accounted for in certain dynamical investigations and ephemeris predictions. Periodic TTVs with timescales short compared to the interval of Kepler observations largely average out and do not produce significant errors in estimates of orbital periods. TTVs with timescales comparable to the interval of Kepler observations have been fit for dozens of Kepler planet candidates to estimate long-term average orbital periods (Holczer et al. 2016, ApJS 225, 9), and more detailed dynamical models have been used to estimate long-term average periods of a small number of well-studied planets. Most Kepler planets that show large TTVs are near mean-motion orbital resonances with other planets. The largest effect for planets moderately close to two-body resonances are due to rotation of the forced eccentricity vector by resonant perturbations; the timescale for this precession is typically short, so the variations tend to average out. Libration of planets locked in resonances typically occurs on timescales longer than the Kepler baseline, but few Kepler planets appear to be resonantly-locked. The more general but smaller (during the era of Kepler observations) effect is caused by secular precession of the planets' free eccentricities usually has a period much longer than the baseline of the Kepler observations, so it is not accounted for in estimates of mean periods or uncertainties. This precession causes a discrepancy between Kepler era mean orbital period and long-term mean orbital period can exist even for planets having TTVs that are too small to be observable during the epoch of Kepler observations.

Kepler↗
NASA NTRS1994

A preliminary evaluation of ocean topography from the TOPEX/POSEIDON mission

We have analyzed 50 ten-day cycles of TOPEX/POSEIDON (T/P) altimeter data to evaluate the ocean dynamic topography and its temporal variations. We have employed data from both the U.S. and French altimeters along with the NASA precision orbits in this analysis. Errors in the diurnal and semidiurnal components of the Cartwright-Ray tide model have been significantly reduced using a correction developed from a combination of JGM-2 and OSU91A was employed, as well as a geoid model based solely on OSU91A. The long wavelengths of the comparisons to historical data, although geoid error still corrupts the dynamic topography for wavelengths shorter than 2500 km. The root mean square (RMS) variability is similar to previous results from Geosat, with bakground 'noise' approaching 3 cm RMS. The computed annual and semiannual variations are also similar to previous Geosat results, although the hemispheric distribution of the annual heating cycle is much better presented in the T/P results. They also compare reasonably well with the Levitus hydrographic compilation in the northern hemisphere, although the T/P variations generally have larger amplitudes. Ten-day average maps of variations in sea level compare well with simulations measurements at ocean tide gauges, with RMS differences of less than 4 cm and correlations greater than 0.6 for most of the island gauges. Time-longitude plots of these sea level variations at different latitudes in the Pacific clearly show the presence of equatorial Kelvin waves and Rossby waves, with the wave speeds agreeing well with theoretical and observed values. Measurement of variations in global sea level over cycles 2-51 have an RMS variability of 6.3 mm and a rate of change of -3.5 +/- 8 mm/yr, the uncertainty primarily due to insufficient averaging of the interannual and periodic sea level variations. These results show that the accuracy of the T/P measurements of sea level has dramatically improved over previous missions, with estimated time variable errors of 4 cm or less. Although geographically correlated orbit errors have also been reduced to the few centimeter level, further improvement in determinations of the mean dynamic topography will be difficult to obtain until a more accurate model of the marine geoid is available.

Nerem, R. S.↗
NASA NTRS2000

Soil Moisture Memory in Climate Models

Water balance considerations at the soil surface lead to an equation that relates the autocorrelation of soil moisture in climate models to (1) seasonality in the statistics of the atmospheric forcing, (2) the variation of evaporation with soil moisture, (3) the variation of runoff with soil moisture, and (4) persistence in the atmospheric forcing, as perhaps induced by land atmosphere feedback. Geographical variations in the relative strengths of these factors, which can be established through analysis of model diagnostics and which can be validated to a certain extent against observations, lead to geographical variations in simulated soil moisture memory and thus, in effect, to geographical variations in seasonal precipitation predictability associated with soil moisture. The use of the equation to characterize controls on soil moisture memory is demonstrated with data from the modeling system of the NASA Seasonal-to-Interannual Prediction Project.

Koster, Randal D.↗
NASA NTRS2017

Si III OV Bright Line of Scattering Polarized Light That Has Been Observed in the CLASP and Its Center-to-Limb Variation

The CLASP (Chromospheric Lyman-Alpha Spectro- Polarimeter) rocket experiment, in addition to the ultraviolet region of the Ly alpha emission line (121.57 nm), emission lines of Si III (120.65 nm) and OV (121.83 nm) is can be observed. These are optically thin line compared to a Ly alpha line, if Rarere captured its polarization, there is a possibility that dripping even a new physical diagnosis chromosphere-transition layer. In particular, OV bright light is a release from the transition layer, further, three P one to one S(sub 0) is a forbidden line (cross-triplet transition between lines), it was not quite know whether to polarization.

spectropolarimeter↗
NASA NTRS1982

Predicted diurnal variations of electron density for three high-latitude incoherent scatter radars

A high-latitude ionospheric model is used to predict the diurnal variations of electron density which should be observed by the EISCAT, Chatanika, and Millstone Hill incorporated scatter facilities. The calculations take into account a strong convection model without substorms. The provided electron density predictions should be used to obtain an indication of the quantitative differences in measured electron density that are to be expected when the three radars probe the high-latitude ionosphere simultaneously. These differences vary with altitude, latitude, local time, and season, and are associated with the UT dependence of the high-latitude ionosphere which results from the offset between the geomagnetic and geographic poles. It was found that the three facilities should observe the greatest difference in electron density variations in winter.

Sojka, J. J.↗
NASA NTRS1984

New evidence on the nature of the neutron star and accretion flow in Vela X-1 from pulse timing observations

It is believed that variations in the intrinsic pulse frequencies of neutron star X-ray sources reflect changes in the rotation rate of the stellar crust produced by torque originating outside and inside the star. The external torque depends on the flow pattern of the accreting plasma, while the internal torque depends on the state of the interior and its coupling to the crust. Information about the accretion flow and the star itself can, therefore, be obtained on the basis of a study of intrinsic frequency variations. The present investigation is concerned with the results of a detailed study of the 283 s pulsing X-ray star Vela X-1 using pulse timing techniques. It is found that the frequency variations in this source are consistent with the occurrence of a succession of temporally unresolved events which can be characterized mathematically as second-order red noise in the pulse phase.

Boynton, P. E.↗
NASA NTRS1998

{Interball-1 Plasma, Magnetic Field, and Energetic Particle Observations}

Funding from NASA was received in two installments. The first installment supported research using Russian/Czech/Slovak/French Interball-1 plasma, magnetic field, and energetic particles observations in the vicinity of the magnetopause. The second installment provided salary support to review unsolicited proposals to NASA for data recovery and archiving, and also to survey ISTP data provision efforts. Two papers were published under the auspices of the grant. Sibeck et al. reported Interball-1 observations of a wave on the magnetopause with an amplitude in excess of 5 R(sub E), the largest ever reported to date. They attributed the wave to a hot flow anomaly striking the magnetopause and suggested that the hot flow anomaly itself formed during the interaction of an IMF discontinuity with the bow shock. Nemecek et al. used Interball-1's VDP Faraday cup to identify large transient increases in the magnetosheath density. They noted large variations in simultaneous Wind observations of the IMF cone angle, but were unable to establish any relationship between the cone angle variations at Wind and the density variations at Interball-1. Funds from the second installment were used to review over 20 proposals from various researchers in the scientific community who sought NASA support to restore or archive past observations. It also supported a survey of ISTP data provisions which was used as input to a Senior Review of ongoing NASA ISTP programs.

Sibeck, David G.↗
NASA NTRS2012

Multi-Sensors Observations of Pre-Earthquake Signals. What We Learned from the Great Tohoku Earthquake?

The lessons learned from the Great Tohoku EQ (Japan, 2011) will affect our future observations and an analysis is the main focus of this presentation. Multi-sensors observations and multidisciplinary research is presented in our study of the phenomena preceding major earthquakes Our approach is based on a systematic analysis of several physical and environmental parameters, which been reported by others in connections with earthquake processes: thermal infrared radiation; temperature; concentration of electrons in the ionosphere; radon/ion activities; and atmospheric temperature/humidity [Ouzounov et al, 2011]. We used the Lithosphere-Atmosphere-Ionosphere Coupling (LAIC) model, one of several possible paradigms [Pulinets and Ouzounov, 2011] to interpret our observations. We retrospectively analyzed the temporal and spatial variations of three different physical parameters characterizing the state of the atmosphere, ionosphere the ground surface several days before the March 11, 2011 M9 Tohoku earthquake Namely: (i) Outgoing Long wave Radiation (OLR) measured at the top of the atmosphere; (ii) Anomalous variations of ionospheric parameters revealed by multi-sensors observations; and (iii) The change in the foreshock sequence (rate, space and time); Our results show that on March 8th, 2011 a rapid increase of emitted infrared radiation was observed and an anomaly developed near the epicenter with largest value occurring on March 11 at 07.30 LT. The GPS/TEC data indicate an increase and variation in electron density reaching a maximum value on March 8. Starting from this day in the lower ionosphere there was also observed an abnormal TEC variation over the epicenter. From March 3 to 11 a large increase in electron concentration was recorded at all four Japanese ground-based ionosondes, which returned to normal after the main earthquake. We use the Japanese GPS network stations and method of Radio Tomography to study the spatiotemporal structure of ionospheric perturbations, and to distinguish ionospheric responses to processes of EQ preparation against the effects of other factors. The 2-D snapshots of the electron density over Japan showed abnormal increase over the maximum stress during the night, a few hours before the main shock. Our results from recording atmospheric and ionospheric conditions during the earthquake indicate the presence of anomalies in the atmosphere and ionospheres occurring consistently over regions of maximum stress near the epicenter. Due to their long duration (hours and days) and spatial appearance (only over the Sendai region) these results do not appear to be caused by meteorological or magnetic activity. They reveal the existence of atmospheric and ionospheric phenomena occurring prior to the earthquake, which indicates new evidence of a distinct coupling between the lithosphere and atmosphere/ionosphere. Similar results have been reported before the catastrophic events in Chile (M8.8, 2010), Italy (M6.3, 2009) and Sumatra (M9.3, 2004).

Ouzonounov, D.↗