Results for “coordination complex” — Page 96

DOE OSTI2017

Dynamic cellular finite-element method for modelling large-scale cell migration and proliferation under the control of mechanical and biochemical cues: a study of re-epithelialization

Computational modelling of cells can reveal insight into the mechanisms of the important processes of tissue development. However, current cell models have limitations and are challenged to model detailed changes in cellular shapes and physical mechanics when thousands of migrating and interacting cells need to be modelled. In this paper we describe a novel dynamic cellular finite-element model (DyCelFEM), which accounts for changes in cellular shapes and mechanics. It also models the full range of cell motion, from movements of individual cells to collective cell migrations. The transmission of mechanical forces regulated by intercellular adhesions and their ruptures are also accounted for. Intra-cellular protein signalling networks controlling cell behaviours are embedded in individual cells. We employ DyCelFEM to examine specific effects of biochemical and mechanical cues in regulating cell migration and proliferation, and in controlling tissue patterning using a simplified re-epithelialization model of wound tissue. Our results suggest that biochemical cues are better at guiding cell migration with improved directionality and persistence, while mechanical cues are better at coordinating collective cell migration. Finally and overall, DyCelFEM can be used to study developmental processes when a large population of migrating cells under mechanical and biochemical controls experience complex changes in cell shapes and mechanics.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2024

An interaction network in the polymerase active site is a prerequisite for Watson-Crick base pairing in Pol γ

The replication accuracy of DNA polymerase gamma (Pol γ) is essential for mitochondrial genome integrity. Mutation of human Pol γ arginine-853 has been linked to neurological diseases. Although not a catalytic residue, Pol γ arginine-853 mutants are void of polymerase activity. To identify the structural basis for the disease, we determined a crystal structure of the Pol γ mutant ternary complex with correct incoming nucleotide 2'-deoxycytidine 5'-triphosphate (dCTP). Opposite to the wild type that undergoes open-to-closed conformational changes when bound to a correct nucleotide that is essential for forming a catalytically competent active site, the mutant complex failed to undergo the conformational change, and the dCTP did not base pair with its Watson-Crick complementary templating residue. Our studies revealed that arginine-853 coordinates an interaction network that aligns the 3'-end of primer and dCTP with the catalytic residues. Disruption of the network precludes the formation of Watson-Crick base pairing and closing of the active site, resulting in an inactive polymerase.

59 BASIC BIOLOGICAL SCIENCES↗

DOE OSTI2026

Predictive analytics to direct clinical attention to complex patients with elevated suicide risk: enhancement of the Veterans Health Administration REACH VET model

Suicide is a major public health concern, particularly among Veterans. The U.S. Department of Veterans Affairs Veterans Health Administration (VHA) employs the Recovery Engagement and Coordination for Health–Veterans Enhanced Treatment (REACH VET) model to prioritise high-risk patients for targeted clinical attention. REACH VET 1.0 (RV 1.0) was developed on 2008–2011 data. To reflect changes in clinical practice and populations, VHA updated it to REACH VET 2.0 (RV 2.0). This study describes its development and validation. RV 2.0 used longitudinal data from 7,248,170 VHA patients (4,967 suicide deaths) in 2018–2019, with 650 time-varying demographic, clinical and area-level predictors derived from a 2-year lookback (2016–2019). An ensemble of Elastic-Net logistic regression models was trained on 2018 data and evaluated monthly at the population level in 2019, focusing on the top 0.1% intervention risk tier. Analyses assessed model discrimination, suicide detection, risk concentration, subgroup consistency (sex, age and race/ethnicity) and performance relative to RV 1.0 using the same percentile-based risk strata. RV 2.0 outperformed RV 1.0 across all risk strata, with better discrimination (C-statistic 0.76 vs 0.69) and consistent performance across demographic subgroups. Within the top 0.1% of predicted risk, RV 2.0 identified more deaths, higher suicide rates and greater mortality risk concentration both when averaged across the 12 monthly 2019 test sets (5.6 vs 3.6; 83.6 vs 53.7 per 100,000 person-years; 21.0 vs 14.1) and when annualised for 2019 (67 vs 43; 2.7% vs 1.7%; 1,003 vs 644 per 100,000 person-years; 26.7 vs 17.1). RV 2.0 improves suicide risk stratification among Veterans, demonstrating better performance and consistent prediction across subgroups and highlighting the need for regular model updates and evaluation.

Peluso, Alina [Oak Ridge National Laboratory (ORNL↗

DOE OSTI2021

Nuclear Material Process Modeling at the Y-12 National Security Complex

Dynamic simulation modeling is used at Y-12 to evaluate and forecast nuclear material inventories and production capacities to ensure that future supply can meet mission demand. Model outputs are analyzed by numerous Y-12 organizations and programs and coordinated with NNSA’s Office of Secondary Stage Production Modernization. Data-driven decisions for both short-term and long-term strategic planning for Y-12 mission execution are informed by the model. Dynamic simulation modeling capabilities for Y-12 nuclear material production continue to be expanded and refined.

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

DOE OSTI2025

Architecting the Grid Edge: Ensuring Reliability and Resilience

Changes in technology, customer expectations, and business and regulatory environments are rapidly evolving causing fundamental changes in the nation’s electrical infrastructure. Nowhere is this more apparent that at the “grid edge”, where there is an increasing number of new devices and systems, as well as complex new interactions between them. This is leading to the traditional relationship between the end-use customers and their utilities being expanded by an increasing number of stakeholders, each with their own operational and financial objectives, governed by regulatory policy. While there are concerns about the rapidly increasing complexity negatively impacting reliable and resilience of the electrical infrastructure, these changes are also bringing new resources and opportunities that hold great potential if they can be properly coordinated. This white paper outlines the considerations for the coordination of multi-stakeholder objectives with electric utility requirements using the concept of grid services. Describing a framework that enables new stakeholders to achieve their local technical and economic objectives, while simultaneously delivering operational benefits to the electrical infrastructure. The concepts of grid architecture are presented as a tool to evaluate how stakeholders might participate in, and benefit from, services, and how utilities can make decision on the reliance on services to ensure reliability and resilience, translating abstract concepts into actionable information for utilities and grid edge stakeholders. The end result of proper coordination, informed by grid architecture, will be a range of new devices and systems, operated by new stakeholders, achieving their local objectives while also increasing the reliability, resilience, security, and affordability of the nation’s critical electrical infrastructure.

24 POWER TRANSMISSION AND DISTRIBUTION↗

DOE OSTI2025

Architecting the Grid Edge: Ensuring Reliability and Resilience

Changes in technology, customer expectations, and business and regulatory environments are rapidly evolving causing fundamental changes in the nation’s electrical infrastructure. Nowhere is this more apparent that at the “grid edge”, where there is an increasing number of new devices and systems, as well as complex new interactions between them. This is leading to the traditional relationship between the end-use customers and their utilities being expanded by an increasing number of stakeholders, each with their own operational and financial objectives, governed by regulatory policy. While there are concerns about the rapidly increasing complexity negatively impacting reliable and resilience of the electrical infrastructure, these changes are also bringing new resources and opportunities that hold great potential if they can be properly coordinated. This white paper outlines the considerations for the coordination of multi-stakeholder objectives with electric utility requirements using the concept of grid services. Describing a framework that enables new stakeholders to achieve their local technical and economic objectives, while simultaneously delivering operational benefits to the electrical infrastructure. The concepts of grid architecture are presented as a tool to evaluate how stakeholders might participate in, and benefit from, services, and how utilities can make decision on the reliance on services to ensure reliability and resilience, translating abstract concepts into actionable information for utilities and grid edge stakeholders. The end result of proper coordination, informed by grid architecture, will be a range of new devices and systems, operated by new stakeholders, achieving their local objectives while also increasing the reliability, resilience, security, and affordability of the nation’s critical electrical infrastructure.

24 POWER TRANSMISSION AND DISTRIBUTION↗

NASA NTRS1989

NASA-Enhanced Version Of Automatically Programmed Tool Software (APT)

APT code one of most widely used software tools for complex numerically-controlled machining. Both a programming language and software that processes language. Upgrades include super pocket for concave polygon pockets and editor to reprocess cutter location coordinates according to user-supplied commands.

Purves, L. R.↗

NASA NTRS1989

Integrated aerodynamic-structural design of a forward-swept transport wing

The introduction of composite materials is having a profound effect on aircraft design. Since these materials permit the designer to tailor material properties to improve structural, aerodynamic and acoustic performance, they require an integrated multidisciplinary design process. Futhermore, because of the complexity of the design process, numerical optimization methods are required. The utilization of integrated multidisciplinary design procedures for improving aircraft design is not currently feasible because of software coordination problems and the enormous computational burden. Even with the expected rapid growth of supercomputers and parallel architectures, these tasks will not be practical without the development of efficient methods for cross-disciplinary sensitivities and efficient optimization procedures. The present research is part of an on-going effort which is focused on the processes of simultaneous aerodynamic and structural wing design as a prototype for design integration. A sequence of integrated wing design procedures has been developed in order to investigate various aspects of the design process.

Haftka, Raphael T.↗

NASA NTRS2005

Critical Propulsion Components: Exhaust Nozzle - Volume 3

Several studies have concluded that a supersonic aircraft, if environmentally acceptable and economically viable, could successfully compete in the 21st century marketplace. However, before industry can commit to what is estimated as a 15 to 20 billion dollar investment, several barrier issues must be resolved. In an effort to address these barrier issues, NASA and Industry teamed to form the High-Speed Research (HSR) program. As part of this program, the Critical Propulsion Components (CPC) element was created and assigned the task of developing those propulsion component technologies necessary to: (1) reduce cruise emissions by a factor of 10 and (2) meet the ever-increasing airport noise restrictions with an economically viable propulsion system. The CPC-identified critical components were ultra-low emission combustors, low-noise/high-performance exhaust nozzles, low-noise fans, and stable/high-performance inlets. Propulsion cycle studies (coordinated with NASA Langley Research Center sponsored airplane studies) were conducted throughout this CPC program to help evaluate candidate components and select the best concepts for the more complex and larger scale research efforts. The propulsion cycle and components ultimately selected were a mixed-flow turbofan (MFTF) engine employing a lean, premixed, prevaporized (LPP) combustor coupled to a two-dimensional mixed compression inlet and a two-dimensional mixer/ejector nozzle. Due to the large amount of material presented in this report, it was prepared in four volumes; Volume 1: Summary, Introduction, and Propulsion System Studies, Volume 2: Combustor, Volume 3: Exhaust Nozzle, and Volume 4: Inlet and Fan/Inlet Acoustic Team.

Source record↗

NASA NTRS2005

Critical Propulsion Components: Inlet and Fan/Inlet Accoustics Team - Volume 4

Several studies have concluded that a supersonic aircraft, if environmentally acceptable and economically viable, could successfully compete in the 21st century marketplace. However, before industry can commit to what is estimated as a 15 to 20 billion dollar investment, several barrier issues must be resolved. In an effort to address these barrier issues, NASA and Industry teamed to form the High-Speed Research (HSR) program. As part of this program, the Critical Propulsion Components (CPC) element was created and assigned the task of developing those propulsion component technologies necessary to: (1) reduce cruise emissions by a factor of 10 and (2) meet the ever-increasing airport noise restrictions with an economically viable propulsion system. The CPC-identified critical components were ultra-low emission combustors, low-noise/high-performance exhaust nozzles, low-noise fans, and stable/high-performance inlets. Propulsion cycle studies (coordinated with NASA Langley Research Center sponsored airplane studies) were conducted throughout this CPC program to help evaluate candidate components and select the best concepts for the more complex and larger scale research efforts. The propulsion cycle and components ultimately selected were a mixed-flow turbofan (MFTF) engine employing a lean, premixed, prevaporized (LPP) combustor coupled to a two-dimensional mixed compression inlet and a two-dimensional mixer/ejector nozzle. Due to the large amount of material presented in this report, it was prepared in four volumes; Volume 1: Summary, Introduction, and Propulsion System Studies, Volume 2: Combustor, Volume 3: Exhaust Nozzle, and Volume 4: Inlet and Fan/Inlet Acoustic Team.

Source record↗

NASA NTRS2005

Critical Propulsion Components: Summary, Introduction, and Propulsion Systems Studies - Volume 1

Several studies have concluded that a supersonic aircraft, if environmentally acceptable and economically viable, could successfully compete in the 21st century marketplace. However, before industry can commit to what is estimated as a 15 to 20 billion dollar investment, several barrier issues must be resolved. In an effort to address these barrier issues, NASA and Industry teamed to form the High-Speed Research (HSR) program. As part of this program, the Critical Propulsion Components (CPC) element was created and assigned the task of developing those propulsion component technologies necessary to: (1) reduce cruise emissions by a factor of 10 and (2) meet the ever-increasing airport noise restrictions with an economically viable propulsion system. The CPC-identified critical components were ultra-low emission combustors, low-noise/high-performance exhaust nozzles, low-noise fans, and stable/high-performance inlets. Propulsion cycle studies (coordinated with NASA Langley Research Center sponsored airplane studies) were conducted throughout this CPC program to help evaluate candidate components and select the best concepts for the more complex and larger scale research efforts. The propulsion cycle and components ultimately selected were a mixed-flow turbofan (MFTF) engine employing a lean, premixed, prevaporized (LPP) combustor coupled to a two-dimensional mixed compression inlet and a two-dimensional mixer/ejector nozzle. Due to the large amount of material presented in this report, it was prepared in four volumes; Volume 1: Summary, Introduction, and Propulsion System Studies, Volume 2: Combustor, Volume 3: Exhaust Nozzle, and Volume 4: Inlet and Fan/ Inlet Acoustic Team.

Source record↗

NASA NTRS2005

Critical Propulsion Components: Combustor - Volume 2

Several studies have concluded that a supersonic aircraft, if environmentally acceptable and economically viable, could successfully compete in the 21st century marketplace. However, before industry can commit to what is estimated as a 15 to 20 billion dollar investment, several barrier issues must be resolved. In an effort to address these barrier issues, NASA and Industry teamed to form the High-Speed Research (HSR) program. As part of this program, the Critical Propulsion Components (CPC) element was created and assigned the task of developing those propulsion component technologies necessary to: (1) reduce cruise emissions by a factor of 10 and (2) meet the ever-increasing airport noise restrictions with an economically viable propulsion system. The CPC-identified critical components were ultra-low emission combustors, low-noise/high-performance exhaust nozzles, low-noise fans, and stable/high-performance inlets. Propulsion cycle studies (coordinated with NASA Langley Research Center sponsored airplane studies) were conducted throughout this CPC program to help evaluate candidate components and select the best concepts for the more complex and larger scale research efforts. The propulsion cycle and components ultimately selected were a mixed-flow turbofan (MFTF) engine employing a lean, premixed, prevaporized (LPP) combustor coupled to a two-dimensional mixed compression inlet and a two-dimensional mixer/ejector nozzle. Due to the large amount of material presented in this report, it was prepared in four volumes; Volume 1: Summary, Introduction, and Team. Propulsion System Studies, Volume 2: Combustor, Volume 3: Exhaust Nozzle, and Volume 4: Inlet and Fan/Inlet Acoustic Team.

Source record↗

NASA NTRS2009

Synthesis and Control of Flexible Systems with Component-Level Uncertainties

An efficient and computationally robust method for synthesis of component dynamics is developed. The method defines the interface forces/moments as feasible vectors in transformed coordinates to ensure that connectivity requirements of the combined structure are met. The synthesized system is then defined in a transformed set of feasible coordinates. The simplicity of form is exploited to effectively deal with modeling parametric and non-parametric uncertainties at the substructure level. Uncertainty models of reasonable size and complexity are synthesized for the combined structure from those in the substructure models. In particular, we address frequency and damping uncertainties at the component level. The approach first considers the robustness of synthesized flexible systems. It is then extended to deal with non-synthesized dynamic models with component-level uncertainties by projecting uncertainties to the system level. A numerical example is given to demonstrate the feasibility of the proposed approach.

Maghami, Peiman G.↗

NASA NTRS2012

Cloud Computing for Mission Design and Operations

The space mission design and operations community already recognizes the value of cloud computing and virtualization. However, natural and valid concerns, like security, privacy, up-time, and vendor lock-in, have prevented a more widespread and expedited adoption into official workflows. In the interest of alleviating these concerns, we propose a series of guidelines for internally deploying a resource-oriented hub of data and algorithms. These guidelines provide a roadmap for implementing an architecture inspired in the cloud computing model: associative, elastic, semantical, interconnected, and adaptive. The architecture can be summarized as exposing data and algorithms as resource-oriented Web services, coordinated via messaging, and running on virtual machines; it is simple, and based on widely adopted standards, protocols, and tools. The architecture may help reduce common sources of complexity intrinsic to data-driven, collaborative interactions and, most importantly, it may provide the means for teams and agencies to evaluate the cloud computing model in their specific context, with minimal infrastructure changes, and before committing to a specific cloud services provider.

cloud computing↗

NASA NTRS2012

Development of Waypoint Planning Tool in Response to NASA Field Campaign Challenges

Airborne real time observations are a major component of NASA's Earth Science research and satellite ground validation studies. For mission scientists, planning a research aircraft mission within the context of meeting the science objectives is a complex task because it requires real time situational awareness of the weather conditions that affect the aircraft track. Multiple aircrafts are often involved in NASA field campaigns. The coordination of the aircrafts with satellite overpasses, other airplanes and the constantly evolving, dynamic weather conditions often determines the success of the campaign. A flight planning tool is needed to provide situational awareness information to the mission scientists, and help them plan and modify the flight tracks. Scientists at the University of Alabama-Huntsville and the NASA Marshall Space Flight Center developed the Waypoint Planning Tool, an interactive software tool that enables scientists to develop their own flight plans (also known as waypoints) with point -and-click mouse capabilities on a digital map filled with real time raster and vector data. The development of this Waypoint Planning Tool demonstrates the significance of mission support in responding to the challenges presented during NASA field campaigns. Analysis during and after each campaign helped identify both issues and new requirements, and initiated the next wave of development. Currently the Waypoint Planning Tool has gone through three rounds of development and analysis processes. The development of this waypoint tool is directly affected by the technology advances on GIS/Mapping technologies. From the standalone Google Earth application and simple KML functionalities, to Google Earth Plugin and Java Web Start/Applet on web platform, and to the rising open source GIS tools with new JavaScript frameworks, the Waypoint Planning Tool has entered its third phase of technology advancement. The newly innovated, cross ]platform, modular designed JavaScript ]controlled Way Point Tool is planned to be integrated with NASA Airborne Science Mission Tool Suite. Adapting new technologies for the Waypoint Planning Tool ensures its success in helping scientists reach their mission objectives. This presentation will discuss the development processes of the Waypoint Planning Tool in responding to field campaign challenges, identify new information technologies, and describe the capabilities and features of the Waypoint Planning Tool with the real time aspect, interactive nature, and the resultant benefits to the airborne science community.

He, Matt↗

NASA NTRSDate not supplied

Advanced Modeling of Control-Structure Interaction in Thrust Vector Control Systems

The Space Launch System (SLS) Core Stage (CS) Thrust Vector Control (TVC) system is comprised of 8 mechanical feedback Shuttle heritage Type III TVC actuators and four RS-25 engines, each attached to a Shuttle heritage gimbal block/bearing. Two actuators are used to move each engine in two planes perpendicular to one another (i.e., pitch and yaw). The TVC system design leverages hardware from the Space Shuttle program as well as new hardware designed specifically for the Core Stage. During the development of the SLS TVC system, a family of advanced dynamics models were developed to extend and compliment the simplified quasi-linear “simplex” model historically used for flight control design and stability analysis. The importance of these advanced models became increasingly evident after ambient and hot fire testing of the Core Stage, which revealed a number of findings associated with the dynamic response of the TVC integrated system. Test responses suggested that the TVC did not meet its performance specifications and its step and frequency responses exhibited unexpected departures from prior lab tests and modeled behavior. One driving factor for these results was a higher-than-expected degree of coupling between the TVC system, the engine dynamics, and the Core Stage structure. This paper is the third installment in a seven-paper series surveying the design, engineering, test validation, and flight performance of the Core Stage Thrust Vector Control system. In this paper, a new method of modeling rocket vehicle thrust vectoring servoelastic dynamics is presented. In this approach, the load dynamics are replaced by a detailed finite element model containing both the rigid body and elastic modes. A partitioning technique is used to compute the effective compliance from the modal data and obtain accurate simulation results using a reduced number of generalized coordinates. Coupled backup structure and nozzle attach compliance effects on multiple engines are captured in higher fidelity than with a spring approximation, eliciting novel effects due to the complex load paths involved in the Core Stage structure. Validation of the model is demonstrated using a variety of structural/modal, laboratory, and full-scale hot fire test data.

Launch Vehicles↗

NASA NTRSDate not supplied

Advanced Modeling of Control-Structure Interaction in Thrust Vector Control Systems

The Space Launch System (SLS) Core Stage (CS) Thrust Vector Control (TVC) system is comprised of 8 mechanical feedback Shuttle heritage Type III TVC actuators and four RS-25 engines, each attached to a Shuttle heritage gimbal block/bearing. Two actuators are used to move each engine in two planes perpendicular to one another (i.e., pitch and yaw). The TVC system design leverages hardware from the Space Shuttle program as well as new hardware designed specifically for the Core Stage. During the development of the SLS TVC system, a family of advanced dynamics models were developed to extend and compliment the simplified quasi-linear “simplex” model historically used for flight control design and stability analysis. The importance of these advanced models became increasingly evident after ambient and hot fire testing of the Core Stage, which revealed a number of findings associated with the dynamic response of the TVC integrated system. Test responses suggested that the TVC did not meet its performance specifications and its step and frequency responses exhibited unexpected departures from prior lab tests and modeled behavior. One driving factor for these results was a higher-than-expected degree of coupling between the TVC system, the engine dynamics, and the Core Stage structure. This paper is the third installment in a seven-paper series surveying the design, engineering, test validation, and flight performance of the Core Stage Thrust Vector Control system. In this paper, a new method of modeling rocket vehicle thrust vectoring servoelastic dynamics is presented. In this approach, the load dynamics are replaced by a detailed finite element model containing both the rigid body and elastic modes. A partitioning technique is used to compute the effective compliance from the modal data and obtain accurate simulation results using a reduced number of generalized coordinates. Coupled backup structure and nozzle attach compliance effects on multiple engines are captured in higher fidelity than with a spring approximation, eliciting novel effects due to the complex load paths involved in the Core Stage structure. Validation of the model is demonstrated using a variety of structural/modal, laboratory, and full-scale hot fire test data.

Launch Vehicles↗

DOE OSTI2023

Entropic control of the free-energy landscape of an archetypal biomolecular machine

Biomolecular machines are complex macromolecular assemblies that utilize thermal and chemical energy to perform essential, multistep, cellular processes. Despite possessing different architectures and functions, an essential feature of the mechanisms of action of all such machines is that they require dynamic rearrangements of structural components. Surprisingly, biomolecular machines generally possess only a limited set of such motions, suggesting that these dynamics must be repurposed to drive different mechanistic steps. Although ligands that interact with these machines are known to drive such repurposing, the physical and structural mechanisms through which ligands achieve this remain unknown. Using temperature-dependent, single-molecule measurements analyzed with a time-resolution-enhancing algorithm, here, we dissect the free-energy landscape of an archetypal biomolecular machine, the bacterial ribosome, to reveal how its dynamics are repurposed to drive distinct steps during ribosome-catalyzed protein synthesis. Specifically, we show that the free-energy landscape of the ribosome encompasses a network of allosterically coupled structural elements that coordinates the motions of these elements. Moreover, we reveal that ribosomal ligands which participate in disparate steps of the protein synthesis pathway repurpose this network by differentially modulating the structural flexibility of the ribosomal complex (i.e., the entropic component of the free-energy landscape). Here, we propose that such ligand-dependent entropic control of free-energy landscapes has evolved as a general strategy through which ligands may regulate the functions of all biomolecular machines. Such entropic control is therefore an important driver in the evolution of naturally occurring biomolecular machines and a critical consideration for the design of synthetic molecular machines.

Science & Technology - Other Topics↗