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At least 19 records

MOA-2007-BLG-400 A Super-Jupiter-mass Planet Orbiting a Galactic Bulge K-dwarf Revealed by Keck Adaptive Optics Imaging

We present Keck/NIRC2 adaptive optics imaging of planetary microlensing event MOA-2007-BLG-400 that resolves the lens star system from the source. We find that the MOA-2007-BLG-400L planetary system consists of a 1.71 ± 0.27M {sub Jup} planet orbiting a 0.69 ± 0.04M {sub ⊙} K-dwarf host star at a distance of 6.89 ± 0.77 kpc from the Sun. So, this planetary system probably resides in the Galactic bulge. The planet–host star projected separation is only weakly constrained due to the close-wide light-curve degeneracy; the 2σ projected separation ranges are 0.6–1.0 au and 4.7–7.7 au for close and wide solutions, respectively. This host mass is at the top end of the range of masses predicted by a standard Bayesian analysis. Our Keck follow-up program has now measured lens-source separations for six planetary microlensing events, and five of these six events have host star masses above the median prediction under the assumption that assumes that all stars have an equal chance of hosting planets detectable by microlensing. This suggests that more massive stars may be more likely to host planets of a fixed mass ratio that orbit near or beyond the snow line. These results also indicate the importance of host star mass measurements for exoplanets found by microlensing. The microlensing survey imaging data from NASA’s Nancy Grace Roman Space Telescope (formerly WFIRST) mission will be doing mass measurements like this for a huge number of planetary events.

47 OTHER INSTRUMENTATION↗

Adaptive optical correction for in vivo two-photon fluorescence microscopy with neural fields

Adaptive optics restore ideal imaging performance in complex samples by measuring and correcting optical aberrations but often require custom-built microscopes with carefully aligned wavefront sensing/shaping devices and can be susceptible to sample motion. Here we describe NeAT, a computational framework using neural fields for adaptive optics two-photon fluorescence microscopy. NeAT estimates wavefront aberration and recovers sample structure from a 3D image stack without requiring external datasets for training. Incorporating motion correction in learning and correcting conjugation errors commonly found in commercial microscopes, NeAT is designed for deployment in biological laboratories for in vivo imaging. We validate NeAT’s performance using a custom-built microscope with a wavefront sensor under varying signal-to-noise ratios, aberration and motion conditions. With a commercial microscope, we demonstrate real-time aberration correction for in vivo morphological and functional imaging in the living mouse brain, with NeAT improving the signal and accuracy of glutamate and calcium imaging of synapses and neurons.

Kang, Iksung↗

Modal focal adaptive optics for Bessel-focus two-photon fluorescence microscopy

Adaptive optics (AO) improves the spatial resolution of microscopy by correcting optical aberrations. While its application has been well established in microscopy modalities utilizing a circular pupil, its adaptation to systems with non-circular pupils, such as Bessel-focus two-photon fluorescence microscopy (2PFM) with an annular pupil, remains relatively uncharted. Herein, we present a modal focal AO (MFAO) method for Bessel-focus 2PFM. Measuring and correcting aberration using a spatial light modulator placed in conjugation with the focal plane of the microscope objective, MFAO employs Zernike annular polynomials — a first in AO implementation — to achieve performance on par with a previous zonal AO method, but with a notably simplified optical configuration. We validated the performance of MFAO in correcting artificial and sample-induced aberrations, as well as in in vivo imaging of zebrafish larvae and mouse brains. By expanding the application of modal AO to annular pupils as well as aberration measurement and correction to a wavefront modulator at the objective focal plane, MFAO represents a notable advancement in the implementation of AO in microscopy.

47 OTHER INSTRUMENTATION↗

Adaptive Optics control GUI

This is a GUI to explore adaptive optics system performance related to closed-loop control theory.

Gerard, BenjaminL↗

Adaptive optical third-harmonic generation microscopy for in vivo imaging of tissues

Third-harmonic generation microscopy is a powerful label-free nonlinear imaging technique, providing essential information about structural characteristics of cells and tissues without requiring external labelling agents. In this work, we integrated a recently developed compact adaptive optics module into a third-harmonic generation microscope, to measure and correct for optical aberrations in complex tissues. Taking advantage of the high sensitivity of the third-harmonic generation process to material interfaces and thin membranes, along with the 1,300-nm excitation wavelength used here, our adaptive optical third-harmonic generation microscope enabled high-resolution in vivo imaging within highly scattering biological model systems. Examples include imaging of myelinated axons and vascular structures within the mouse spinal cord and deep cortical layers of the mouse brain, along with imaging of key anatomical features in the roots of the model plant Brachypodium distachyon. In all instances, aberration correction led to enhancements in image quality.

60 APPLIED LIFE SCIENCES↗

Control Algorithms for Dual-wavefront Sensor Single-conjugate Adaptive Optics

High-contrast imaging systems using active control with adaptive optics (AO) are often limited by non-common path (NCP) aberrations that are seen only at the final science image. AO systems employing focal-plane wavefront sensors (FP-WFSs) are able to simultaneously correct NCP aberrations and measure science images, but they typically require a second stage of control that adds system cost and complexity. We present control algorithms to augment AO systems with FP-WFSs within their existing control setup. We demonstrate inter-arm NCP aberration transfer can be mitigated through temporal filtering, present frequency- and time-domain validation of controller stability and performance, and discuss the optimality of the chosen controllers. This work will enable the development, testing, and installation of FP-WFS technologies for direct imaging of exoplanets.

79 ASTRONOMY AND ASTROPHYSICS↗

Adaptive Optical Two-Photon Fluorescence Microscopy Probes Cellular Organization of Ocular Lenses In Vivo

The mammalian ocular lens is an avascular multicellular organ that grows continuously throughout life. Traditionally, its cellular organization is investigated using dissected lenses, which eliminates in vivo environmental and structural support. Therefore, in vivo optical imaging methods for studying lenses in their native context in live animals are urgently needed. Here, we demonstrated that two-photon fluorescence microscopy can visualize lens cells in vivo. To maintain subcellular resolution at depth, we used adaptive optics to correct aberrations owing to ocular and lens tissues, which led to substantial signal and resolution improvements. Imaging lens cells up to 980 µm deep, we observed novel cellular organizations including suture-associated voids, enlarged vacuoles, and large cavities, contrary to the conventional view of a highly ordered organization. We tracked these features longitudinally over weeks and observed the incorporation of new cells during growth. Taken together, noninvasive longitudinal in vivo imaging of lens morphology using adaptive optics two-photon fluorescence microscopy will allow us to observe the development or alterations of lens cellular organization in living animals directly.

60 APPLIED LIFE SCIENCES↗

Rapidly tunable ultrabroadband flying focus using adaptive optics and an axiparabola

A programmable ultrabroadband flying focus was produced using adaptive optics and an axiparabola with a continuous wave superluminescent diode. A deformable mirror and spatial light modulator were used to add a programmable amount of radial group delay, and an axiparabola was used to create an extended focus by imparting spherical aberration. Three different flying focus trajectories were measured using spectral interferometry, two with uniform velocity and one with an accelerating velocity. Here, the temporal response function width of the flying focus optics alone was calculated to be 52 ± 6 fs, and the focal spot size was measured to be (1.2 ± 0.1) × larger than the diffraction-limited spot size through the extended focus.

Markland, H. S. [University of Rochester, NY (Unit↗

Laboratory demonstration of the prediction of wind-blown turbulence by adaptive optics at 8 kHz with use of LQG control

The low-latency adaptive optical mirror system (LLAMAS) is designed to push the limits on achievable latencies and frame rates. It has 21 subapertures across its pupil. Here, a reformulated version of the linear quadratic Gaussian (LQG) method predictive Fourier control is implemented in LLAMAS; for all modes, it takes just 30 µs to compute. In the testbed, a turbulator mixes hot and ambient air to produce wind-blown turbulence. Wind prediction clearly improves correction when compared to an integral controller. Closed-loop telemetry shows that wind-predictive LQG removes the characteristic “butterfly” and reduces temporal error power by up to a factor of three for mid-spatial frequency modes. Strehl changes seen in focal plane images are consistent with telemetry and the system error budget.

47 OTHER INSTRUMENTATION↗

Adaptive Optics Control GUI for the Habitable Worlds Observatory

This GUI explores various whether various adaptive optics control-related parameter can reach NASA's Habitable Worlds Observatory mission requirement of 10 pm rms over 10 minutes, building off of Fig. 4 in Douglas et al. 2019 (DOI: 10.3847/1538-3881/aaf385).

Gerard, BenjaminL [Lawrence Livermore National Lab↗

Control Algorithms for Multi-Wavefront Sensor Adaptive Optics for Astronomical Exoplanet Imaging

This package consists of simulations of control algorithms being proposed for upcoming adaptive optics projects for exoplanet direct imaging, in which one wavefront affector (deformable mirror) is controlled by two wavefront sensors. Simulations include time-domain behavior under various assumed conditions, parameter optimization routines for the controllers, and stability analysis.

Sengupta, Aditya↗

Large Adaptive Optics Survey for Substellar Objects around Young, Nearby, Low-mass Stars with Robo-AO

We present results from the Large Adaptive optics Survey for Substellar Objects, where the goal is to directly image new substellar companions (<70 M {sub Jup}) at wide orbital separations (≳50 au) around young (≲300 Myr), nearby (<100 pc), low-mass (≈0.1–0.8 M{sub ⊙}) stars. We report on 427 young stars imaged in the visible (i') and near-infrared (J or H ) simultaneously with Robo-AO on the Kitt Peak 2.1 m telescope and later the Maunakea University of Hawaii 2.2 m telescope. To undertake the observations, we commissioned a new infrared camera for Robo-AO that uses a low-noise high-speed SAPHIRA avalanche photodiode detector. We detected 121 companion candidates around 111 stars, of which 62 companions are physically associated based on Gaia DR2 parallaxes and proper motions, another 45 require follow-up observations to confirm physical association, and 14 are background objects. The companion separations range from 2 to 1101 au and reach contrast ratios of 7.7 mag in the near-infrared compared to the primary. The majority of confirmed and pending candidates are stellar companions, with ~5 being potentially substellar and requiring follow-up observations for confirmation. We also detected a 43 ± 9 M {sub Jup} and an 81 ± 5 M {sub Jup} companion that were previously reported. We found 34 of our targets have acceleration measurements detected using Hipparcos–Gaia proper motions. Of those, 58{sub -14}{sup +12}% of the 12 stars with imaged companion candidates have significant accelerations (χ{sup 2}>11.8), while only 23{sub -6}{sup +11}% of the remaining 22 stars with no detected companion have significant accelerations. The significance of the acceleration decreases with increasing companion separation. These young accelerating low-mass stars with companions will eventually yield dynamical masses with future orbit monitoring.

47 OTHER INSTRUMENTATION↗

Adaptive optics for optical microscopy [Invited]

Optical microscopy is widely used to visualize fine structures. When applied to bioimaging, its performance is often degraded by sample-induced aberrations. In recent years, adaptive optics (AO), originally developed to correct for atmosphere-associated aberrations, has been applied to a wide range of microscopy modalities, enabling high- or super-resolution imaging of biological structure and function in complex tissues. Here, we review classic and recently developed AO techniques and their applications in optical microscopy.

59 BASIC BIOLOGICAL SCIENCES↗

A High Angular Resolution Survey of Massive Stars in Cygnus OB2: JHK Adaptive Optics Results from the Gemini Near-Infrared Imager

We present results of a high angular resolution survey of massive OB stars in the Cygnus OB2 association that we conducted with the Near-Infrared Imager camera and ALTAIR adaptive optics system of the Gemini North telescope. We observed 74 O- and early-B-type stars in Cyg OB2 in the JHK infrared bands in order to detect binary and multiple companions. The observations are sensitive to equal-brightness pairs at separations as small as [[Formula]], and progressively fainter companions are detectable out to △K=9 mag at a separation of 2″. This faint contrast limit due to read noise continues out to 10″ near the edge of the detector. We assigned a simple probability of chance alignment to each companion based upon its separation and magnitude difference from the central target star and upon areal star counts for the general star field of Cyg OB2. Companion stars with a field membership probability of less than 1% are assumed to be physical companions. This assessment indicates that 47% of the targets have at least one resolved companion that is probably gravitationally bound. Including known spectroscopic binaries, our sample includes 27 binary, 12 triple, and 9 systems with 4 or more components. These results confirm studies of high-mass stars in other environments that find that massive stars are born with a high-multiplicity fraction. The results are important for the placement of the stars in the Hertzsprung–Russell diagram, the interpretation of their spectroscopic analyses, and for future mass determinations through measurement of orbital motion.

79 ASTRONOMY AND ASTROPHYSICS↗

Compensation of heat load deformations using adaptive optics for the ALS upgrade: a wave optics study

In this paper, a realistic wave optics simulation method has been developed to study how wavefront distortions originating from heat load deformations can be corrected using adaptive X-ray optics. Several planned soft X-ray and tender X-ray insertion-device beamlines in the Advanced Light Source upgrade rely on a common design principle. A flat, first mirror intercepts the white beam; vertical focusing is provided by a variable-line-space monochromator; and horizontal focusing comes from a single, pre-figured, adaptive mirror. A variety of scenarios to cope with thermal distortion in the first mirror are studied by finite-element analysis. The degradation of the intensity distribution at the focal plane is analyzed and the adaptive optics that correct it is modeled. The range of correctable wavefront errors across the operating range of the beamlines is reported in terms of mirror curvature and spatial frequencies. The software developed is a one-dimensional wavefront propagation package made available in the OASYS suite, an adaptable, customizable and efficient beamline modeling platform.

73 NUCLEAR PHYSICS AND RADIATION PHYSICS↗

OGLE-2017-BLG-1434Lb: Confirmation of a Cold Super-Earth Using Keck Adaptive Optics

The microlensing event OGLE-2017-BLG-1434 features a cold super-Earth planet that is 1 of 11 microlensing planets with a planet–host-star mass ratio of q < 1 × 10{sup -4}. We provide an additional mass–distance constraint on the lens host using near-infrared adaptive optics photometry from Keck/NIRC2. We are able to determine a flux excess of K {sub L} = 16.96 ± 0.11, which most likely comes entirely from the lens star. Combining this with constraints from the large Einstein ring radius, θ {sub E} = 1.40 ± 0.09 mas, and OGLE parallax we confirm this event as a super-Earth with a mass of m {sub p} = 4.43 ± 0.25M {sub ⊕}. This system lies at a distance of D {sub L} = 0.86 ± 0.05 kpc from Earth and the lens star has a mass of M {sub L} = 0.234 ± 0.012M {sub ⊙}. We confirm that with a star–planet mass ratio of q = 0.57 × 10{sup -4}, OGLE-2017-BLG-1434 lies near the inflexion point of the planet–host mass-ratio power law.

71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSIC↗

Retinal microvascular and neuronal pathologies probed in vivo by adaptive optical two-photon fluorescence microscopy

The retina, behind the transparent optics of the eye, is the only neural tissue whose physiology and pathology can be non-invasively probed by optical microscopy. The aberrations intrinsic to the mouse eye, however, prevent high-resolution investigation of retinal structure and function in vivo. Optimizing the design of a two-photon fluorescence microscope (2PFM) and sample preparation procedure, we found that adaptive optics (AO), by measuring and correcting ocular aberrations, is essential for resolving putative synaptic structures and achieving three-dimensional cellular resolution in the mouse retina in vivo. Applying AO-2PFM to longitudinal retinal imaging in transgenic models of retinal pathology, we characterized microvascular lesions with sub-capillary details in a proliferative vascular retinopathy model, and found Lidocaine to effectively suppress retinal ganglion cell hyperactivity in a retinal degeneration model. Tracking structural and functional changes at high-resolution longitudinally, AO-2PFM enables microscopic investigations of retinal pathology and pharmacology for disease diagnosis and treatment in vivo.

59 BASIC BIOLOGICAL SCIENCES↗

MUSE adaptive-optics spectroscopy confirms dual active galactic nuclei and strongly lensed systems at sub-arcsec separation

The novel Gaia multi peak (GMP) technique has proven to be able to successfully select dual and lensed active galactic nuclei (AGN) candidates at sub-arcsecond separations. Both populations are important because dual AGN represent one of the central, still largely untested, predictions of ΛCDM cosmology, and compact lensed AGN allow us to probe the central regions of the lensing galaxies. In this work, we present high-spatial-resolution spectroscopy of 12 GMP-selected systems. We used the adaptive-optics assisted integral-field spectrograph MUSE at the VLT to resolve each system and investigate the nature of each component. All targets show the presence of two components confirming the GMP selection. We classify 4 targets as dual AGN, 3 as lensed quasar candidates, and 5 as a chance alignment of a star and an AGN. With separations ranging from 0.30″ to 0.86″, these dual and lensed systems are among the most compact systems discovered to date at z > 0.5. This is the largest sample of distant dual AGN with sub-arcsecond separations ever presented in a single paper.

Scialpi, M. (ORCID:0009000651004986)↗