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At least 19 records

AmeriFlux FLUXNET-1F US-RC2 Cook Agronomy Farm - Conventional Till

This is the AmeriFlux Management Project (AMP) created FLUXNET-1F version of the carbon flux data for the site US-RC2 Cook Agronomy Farm - Conventional Till. This is the FLUXNET version of the carbon flux data for the site US-RC2 Cook Agronomy Farm - Conventional Till produced by applying the standard ONEFlux (1F) software. Site Description - RC2 operated from 2013-2016 at the R.J. Cook Agronomy Farm, as part of a cluster of 5 towers (RC1 to RC5) operated for the Regional Approaches to Climate Change (REACCH) USDA-supported research project. The tower predates the Longterm Agroecosystem Research (LTAR) site common experiment, which was established in nearby fields at the Cook Agronomy Farm in 2017. Cook Agronomy Farm is in the high-precipitation agroecological zone of the Columbia Plateau’s dryland cropping region. Wheat-based crop rotations are grown annually. RC2 had conventional tillage management (reduced-till) since at least 1998. It was contrasted with RC1, which had no-till management over the same time period. RC2 captured the same tillage practices as the US-CF2 site established in 2017 as part of LTAR common experiment. However, the towers have distinct footprints, aspects, and soil series composition.

Chi, Jinshu [The Hong Kong University of Science a↗

AmeriFlux FLUXNET-1F US-RC1 Cook Agronomy Farm - No Till

This is the AmeriFlux Management Project (AMP) created FLUXNET-1F version of the carbon flux data for the site US-RC1 Cook Agronomy Farm - No Till. This is the FLUXNET version of the carbon flux data for the site US-RC1 Cook Agronomy Farm - No Till produced by applying the standard ONEFlux (1F) software. Site Description - RC1 operated from 2013-2016 at the R.J. Cook Agronomy Farm, as part of a cluster of 5 towers (RC1 to RC5) operated for the Regional Approaches to Climate Change (REACCH) USDA-supported research project. The tower predates the Longterm Agroecosystem Research (LTAR) site common experiment, which was established in nearby fields at the Cook Agronomy Farm in 2017. Cook Agronomy Farm is in the high precipitation agroecological zone of the Columbia Plateau’s dryland cropping region. Wheat-based crop rotations are grown on an annual planting schedule. RC1 was in no-till management since 1998, and was contrasted with RC2, which had conventional, reduced-tillage management. RC1 captured the same tillage practices as the US-CF1 site established in 2017 as part of LTAR common experiment. However, the towers have distinct footprints, aspects, and soil series composition.

Chi, Jinshu [The Hong Kong University of Science a↗

AmeriFlux FLUXNET-1F US-CF1 CAF-LTAR Cook East

This is the AmeriFlux Management Project (AMP) created FLUXNET-1F version of the carbon flux data for the site US-CF1 CAF-LTAR Cook East. This is the FLUXNET version of the carbon flux data for the site US-CF1 CAF-LTAR Cook East produced by applying the standard ONEFlux (1F) software. Site Description - CF1 has operated since May 2017 at the R.J. Cook Agronomy Farm outside of Pullman, Washington, and monitors the no-till side of a paired-catchment study that is part of the Longterm Agroecosystem Research (LTAR) site common experiment. CF1 is located in “Cook East,” a field that has been in no-till management since 1998, and represents the Alternative treatment of the LTAR common experiment. It is paired with the CF2 tower location in “Cook West,” which has been in conventional tillage since prior to 1998 and represents the Prevalent treatments of the common experiment. Cook Agronomy Farm is in the high precipitation agroecological zone of the Columbia Plateau’s dryland cropping region. Crop rotations are wheat-based and include winter wheat, spring wheat, chickpea, spring canola, and winter peas. Wheat is the principal cash crop, with other crops grown in rotation for diversity, nutrient, and pest management. Soils are predominantly silt loam texture Mollisols in the Palouse, Thatuna, Naff soil series. The CF1 tower replaced US-RC1, which operated 2012-2016 in a neighboring field that was also in no-till management since 1998. CF1 and RC1 have distinct footprints, aspects, and soil series compositions.

Phillips, Claire L.↗

AmeriFlux FLUXNET-1F US-CF2 CAF-LTAR Cook West

This is the AmeriFlux Management Project (AMP) created FLUXNET-1F version of the carbon flux data for the site US-CF2 CAF-LTAR Cook West. This is the FLUXNET version of the carbon flux data for the site US-CF2 CAF-LTAR Cook West produced by applying the standard ONEFlux (1F) software. Site Description - CF2 has operated since May 2017 at the R.J. Cook Agronomy Farm outside of Pullman, Washington, and monitors the conventionally tilled side of a paired-catchment study that is part of the Longterm Agroecosystem Research (LTAR) site common experiment. CF2 is located in “Cook West,” a field that has been in conventional tillage since prior to 1998 and constitutes the Prevalent treatment of the common experiment. In this case, conventional tillage involves chisel plowing after the winter wheat phase of rotations (approximately every 3-4 years) and occasional harrowing. CF2 is paired with the CF1 tower location in “Cook East,” which has been in no-till since 1998 and represents the Alternative treatment of the common experiment. Both fields are planted in the same crops every year. Cook Agronomy Farm is in the high precipitation agroecological zone of the Columbia Plateau’s dryland cropping region. Crop rotations are wheat-based and include winter wheat, spring wheat, chickpea, spring canola, and winter peas. Wheat is the principal cash crop, with other crops grown in rotation for diversity, nutrient, and pest management. Soils are predominantly silt loam texture Mollisols in the Palouse, Thatuna, Naff soil series. The CF2 tower replaced US-RC2, which operated 2012-2016 in a neighboring field that was also conventionally tilled. CF2 and RC2 have distinct footprints, aspects, and soil series compositions.

Huggins, Dave↗

Meteorological models for estimating phenology of corn

Knowledge of when critical crop stages occur and how the environment affects them should provide useful information for crop management decisions and crop production models. Two sources of data were evaluated for predicting dates of silking and physiological maturity of corn (Zea mays L.). Initial evaluations were conducted using data of an adapted corn hybrid grown on a Typic Agriaquoll at the Purdue University Agronomy Farm. The second phase extended the analyses to large areas using data acquired by the Statistical Reporting Service of USDA for crop reporting districts (CRD) in Indiana and Iowa. Several thermal models were compared to calendar days for predicting dates of silking and physiological maturity. Mixed models which used a combination of thermal units to predict silking and days after silking to predict physiological maturity were also evaluated. At the Agronomy Farm the models were calibrated and tested on the same data. The thermal models were significantly less biased and more accurate than calendar days for predicting dates of silking. Differences among the thermal models were small. Significant improvements in both bias and accuracy were observed when the mixed models were used to predict dates of physiological maturity. The results indicate that statistical data for CRD can be used to evaluate models developed at agricultural experiment stations.

Daughtry, C. S. T.↗

Biomass sorghum production in Iowa, USA

This dataset includes field measurement and model simulation data from Josh Bendorf's (former MS student with Drs. VanLoocke & Heaton, ISU Dept. of Agronomy) thesis project focusing on the simulation of biomass sorghum ( Sorghum bicolor L. Moench) in the state of Iowa. Measured field data are provided in CSV file format for 2019 & 2020. Model output files are provided as NetCDF file format for the state of Iowa (1998-2007) & the larger US region (1969-2018).

Agricultural production systems simulation↗

Enhancing Global Food Security: Opportunities for the American Meteorological Society

Food security is a key pillar of environmental security yet remains one of the world’s greatest challenges. Its obverse, food insecurity, negatively impacts health and well-being, drives mass migration, and undermines national security and global sustainable development. Ensuring food security is a delicate balance of myriad concerns within the atmospheric and Earth sciences, agronomy and agriculture engineering, social sciences, economics, monitoring, and policymaking. A Food Security Presidential Session at the American Meteorological Society’s (AMS) 2022 Annual Meeting brought together experts across disciplines to tackle issues at the nexus of weather, climate, and food security. The starkest takeaway was the realization that, despite its importance and clear roles for the atmospheric and climate sciences, food security has not been a focus for the AMS community. The aim of this paper is to build on the perspectives shared by this expert panel and to identify overlapping issues and key points of intersection between the food-security community and AMS. We examine 1) the interactions between weather, climate, and the food system and how they influence food security; 2) the time and spatial scales of food security decision support that match weather and climate phenomena; 3) the role of both providers and users of information as well as decision-makers in improving research to operations for food security; and 4) the opportunities for the AMS community to address food security. We conclude that, moving forward, the AMS community is well-positioned to scale up its engagement across the global food system to address existing scientific needs and technology gaps to improve global food security.

Food security↗

A life cycle analysis (LCA) primer for the agricultural community

Consumer demand for sustainably produced agricultural products is increasing. One approach that can be used to evaluate and compare product sustainability is to conduct a life cycle analysis (LCA). The term LCA is broadly used to describe a suite of analytical resources and standardized methods. Life cycle analyses are a transdisciplinary tool which can and are used by all professions but work best when conducted by teams with diverse skills and backgrounds. The objective of this paper is to provide a primer on LCAs for the agricultural community and for LCA practitioners unfamiliar with agronomy. Agricultural LCAs can differ greatly because agricultural products have multiple end uses, complex socio-economic and environmental trade-offs, and can be generated using a variety of different practices, resources, and production systems. Through worldwide agricultural marketing, intricate agronomic supply chains and support systems have been developed to produce, store, and distribute agricultural products. In many situations, the production practices are not linked to the products consumers purchase. This limitation can be partially overcome by conducting an LCA, which is a systematic summation of the multiple environmental impacts associated with the production, use, and disposal of a product. However, the large number and types of analyses can make it difficult for non-practitioners and decision-makers to use and interpret LCA results. Finally, it is important for members of the agricultural community to be aware of these analyses and how they can be used and how they affect product markets.

54 ENVIRONMENTAL SCIENCES↗

Heterologous synthesis of the complex homometallic cores of nitrogenase P- and M-clusters in Escherichia coli

Nitrogenase is an active target of heterologous expression because of its importance for areas related to agronomy, energy, and environment. One major hurdle for expressing an active Mo-nitrogenase in Escherichia coli is to generate the complex metalloclusters (P- and M-clusters) within this enzyme, which involves some highly unique bioinorganic chemistry/metalloenzyme biochemistry that is not generally dealt with in the heterologous expression of proteins via synthetic biology; in particular, the heterologous synthesis of the homometallic P-cluster ([Fe 8 S 7 ]) and M-cluster core (or L-cluster; [Fe 8 S 9 C]) on their respective protein scaffolds, which represents two crucial checkpoints along the biosynthetic pathway of a complete nitrogenase, has yet to be demonstrated by biochemical and spectroscopic analyses of purified metalloproteins. Here, we report the heterologous formation of a P-cluster-containing NifDK protein upon coexpression of Azotobacter vinelandii nifD, nifK, nifH, nifM, and nifZ genes, and that of an L-cluster-containing NifB protein upon coexpression of Methanosarcina acetivorans nifB, nifS, and nifU genes alongside the A. vinelandii fdxN gene, in E. coli. Our metal content, activity, EPR, and XAS/EXAFS data provide conclusive evidence for the successful synthesis of P- and L-clusters in a nondiazotrophic host, thereby highlighting the effectiveness of our metallocentric, divide-and-conquer approach that individually tackles the key events of nitrogenase biosynthesis prior to piecing them together into a complete pathway for the heterologous expression of nitrogenase. As such, this work paves the way for the transgenic expression of an active nitrogenase while providing an effective tool for further tackling the biosynthetic mechanism of this important metalloenzyme.

59 BASIC BIOLOGICAL SCIENCES↗

Valuation of pollination services from habitat management: a case study of utility scale solar energy facilities in the United States

Creating and maintaining pollinator habitats following the ecological infrastructure concept in degraded or unutilized land, such as solar energy facilities, is a practical way to synergistically advance the food, energy, and ecology nexus. Given the large land-use requirements for solar farming—the fastest growing renewable energy technology–considerable attention has been focused on strategies to maximize multiple ecosystem services. In this study, we coupled the principles of agronomy and ecology with economics and integrated national-scale data on crops, pollinators, and solar facilities to identify locations for creating pollinator habitats and estimating the economic value of pollination from the habitats. We examined opportunities for pollination services from pollinator-friendly utility-scale solar facilities adjacent to 42 million hectares of pollination-dependent crops in the conterminous United States at high resolution of 1 ha. We used the net income method to estimate the potential economic value of creating habitat in the land adjacent to solar facilities in the eight states with the greatest number of solar installations. Creating pollinator habitats at the 217 utility-scale solar facilities in these states could support adjacent 80,000 hectares of high pollinator dependent crops, which could potentially generate a pollination value of $\$$120 to $\$$264 million USD. The location-specific information and high-resolution maps generated for the United States demonstrate integration of grey and green infrastructure to support the food, energy, and environment nexus.

14 SOLAR ENERGY↗

Beyond denitrification: The role of microbial diversity in controlling nitrous oxide reduction and soil nitrous oxide emissions

Many biotic and abiotic processes contribute to nitrous oxide (N 2 O) production in the biosphere, but N 2 O consumption in the environment has heretofore been attributed primarily to canonical denitrifying microorganisms. The nosZ genes encoding the N 2 O reductase enzyme, NosZ, responsible for N 2 O reduction to dinitrogen are now known to include two distinct groups: the well-studied Clade I which denitrifiers typically possess, and the novel Clade II possessed by diverse groups of microorganisms, most of which are non-denitrifiers. Clade II N 2 O reducers could play an important, previously unrecognized role in controlling N 2 O emissions for several reasons, including: (1) the consumption of N 2 O produced by processes other than denitrification, (2) hypothesized non-respiratory functions of NosZ as an electron sink or for N 2 O detoxification, (3) possible differing enzyme kinetics of Clade II NosZ compared to Clade I NosZ, and (4) greater nosZ gene abundance for Clade II compared to Clade I in soils of many ecosystems. Despite the potential ecological significance of Clade II NosZ, a census of 800 peer-reviewed original research articles discussing nosZ and published from 2013 to 2019 showed that the percentage of articles evaluating or mentioning Clade II nosZ increased from 5% in 2013 to only 22% in 2019. The census revealed that the slowly spreading awareness of Clade II nosZ may result in part from disciplinary silos, with the percentage of nosZ articles mentioning Clade II nosZ ranging from 0% in Agriculture and Agronomy journals to 32% in Multidisciplinary Sciences journals. In addition, inconsistent nomenclature for Clade I nosZ and Clade II nosZ, with 17 different terminologies used in the literature, may have created confusion about the two distinct groups of N 2 O reducers. Furthermore, we provide recommendations to accelerate advances in understanding the role of the diversity of N 2 O reducers in regulating soil N 2 O emissions.

59 BASIC BIOLOGICAL SCIENCES↗

Root traits as drivers of plant and ecosystem functioning: current understanding, pitfalls and future research needs

The effects of plants on the biosphere, atmosphere and geosphere are key determinants of terrestrial ecosystem functioning. However, despite substantial progress made regarding plant belowground components, we are still only beginning to explore the complex relationships between root traits and functions. Drawing on the literature in plant physiology, ecophysiology, ecology, agronomy and soil science, we reviewed 24 aspects of plant and ecosystem functioning and their relationships with a number of root system traits, including aspects of architecture, physiology, morphology, anatomy, chemistry, biomechanics and biotic interactions. Based on this assessment, we critically evaluated the current strengths and gaps in our knowledge, and identify future research challenges in the field of root ecology. Most importantly, we found that belowground traits with the broadest importance in plant and ecosystem functioning are not those most commonly measured. Also, the estimation of trait relative importance for functioning requires us to consider a more comprehensive range of functionally relevant traits from a diverse range of species, across environments and over time series. Finally, we also advocate that establishing causal hierarchical links among root traits will provide a hypothesis-based framework to identify the most parsimonious sets of traits with the strongest links on functions, and to link genotypes to plant and ecosystem functioning.

54 ENVIRONMENTAL SCIENCES↗

Heterologous synthesis of a simplified nitrogenase analog in Escherichia coli

The heterologous synthesis of a nitrogen-fixing system in a non-diazotrophic organism is a long-sought-after goal because of the crucial importance of nitrogenase for agronomy, energy, and the environment. Here, we report the heterologous synthesis of a two-component nitrogenase analog fromAzotobacter vinelandii, which consists of the reductase component (NifH) and the cofactor maturase (NifEN), inEscherichia coli. Metal, electron paramagnetic resonance, and activity analyses verify the cluster composition and functional competence of the heterologously expressed NifH and NifEN. Nuclear magnetic resonance, nanoscale secondary ion mass spectrometry, and growth experiments further illustrate the ability of the NifH/NifEN system to reduce N 2 and incorporate the reduced N into the cellular mass. These results establish NifEN/NifH as a simplified nitrogenase analog that could be optimized and engineered to facilitate transgenic expression and biotechnological adaptations of this important metalloenzyme.

Science & Technology - Other Topics↗

Data for Identifying the best high-biomass sorghum hybrids based on biomass yield potential and feedstock quality affected by nitrogen fertility management under various environments

Data were collected from agronomy fields in Urbana and Ewing, IL, during the 2022 and 2023 growing seasons. The dataset includes dry biomass yield, nitrogen, phosphorus, and potassium concentrations and removals, and chemical composition elements (cellulose, hemicellulose, lignin, and soluble fractions) for 13 high-biomass sorghum hybrids. data_sharing.xlsx contains 20 columns and 104 rows. Below is the explanation of all variables in the file: Year: 2022; 2023 Location: Urbana, IL; Ewing, IL N rate (kg-N/ha): 0; 112 Hybrid #: H1-H13 Pedigree: Pedigree for 13 hybrids Dry biomass yield (Mg/ha): Aboveground dry biomass yield N (g/kg): Nitrogen concentration in plant tissue P (g/kg): Phosphorus concentration in plant tissue K (g/kg): Potassium concentration in plant tissue N (kg/ha): Nitrogen removal by aboveground biomass P (kg/ha): Phosphorus removal by aboveground biomass K (kg/ha): Potassium removal by aboveground biomass Cellulose (g/kg): Cellulose concentration in plant tissue Hemicellulose (g/kg): Hemicellulose concentration in plant tissue Lignin (g/kg): Lignin concentration in plant tissue Soluble (g/kg): Soluble concentration in plant tissue Cellulose (Mg/ha): Cellulose content in aboveground biomass Hemicellulose (Mg/ha): Hemicellulose content in aboveground biomass Lignin (Mg/ha): Lignin content in aboveground biomass Soluble (Mg/ha): Soluble content in aboveground biomass

environmental adaptability↗

Agro-IBIS/THMB Modeled Yield, Nitrogen Loss, and Profitability Maps for the Raccoon River Basin, Iowa, USA

This data was used to assess the potential for improving water quality in this watershed when integrating miscanthus, a bioenergy perennial grass, on to areas of low profit under current corn-soybean management and areas of high nitrogen leaching under both current and future climate conditions. This dataset includes all observations (yield, streamflow, nitrogen loads) and model simulation data used from Dr. Kelsie Ferin’s (former PhD student with Dr. Andy VanLoocke, ISU Dept. of Agronomy) dissertation and publication in Global Change Biology – Bioenergy (DOI: 10.1111/gcbb.13078). Modeled output included in this dataset includes corn, soybean, and miscanthus yields, nitrogen leaching rates, net mineralization rates, land use cover fractions with and without the inclusion of miscanthus, profitability maps, additional soil property maps, and simulated streamflow and nitrogen loads near the outlet of the Raccoon River Basin (Van Meter, Iowa) for both historical and future climate conditions. This dataset includes a mixture of netCDF, .tiff, .csv, and .xlsx files. See README.pdf for more information.

Ferin, Kelsie↗