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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 19 records
All sourcesNASA NTRSDOE OSTI
DOE OSTI2018

Loss of virulence in NAD(P)H cytochrome b5 oxidoreductase deficient Leishmania major

Highlights: • Linoleate is an absolute requirement for Leishmania promastigote survival. • Linoleate deficient Leishmania shows avirulence. • Parasite derived linoleate inactivates NF-κB signaling pathway. • Leishmanial linoleate inhibits both COX-2 and TNF-α expression in macrophage. • Supplementing external linoleate reverts most of the observed phenotypes. Leishmania promastigotes have the ability to synthesize essential polyunsaturated fatty acids de novo and can grow in lipid free media. Recently, we have shown that NAD(P)H cytochrome b5 oxidoreductase (Ncb5or) enzyme in Leishmania acts as the redox partner for Δ12 fatty acid desaturase, which catalyses the conversion of oleate to linoleate. So far, the exact role of Leishmania derived linoleate synthesis is still incomplete in the literature. The viability assay by flow cytometry as well as microscopic studies suggests that linoleate is an absolute requirement for Leishmania promastigote survival in delipidated media. Western blot analysis suggested that infection with log phase linoleate deficient mutant (KO) results in increased level of NF-κBp65, IκB and IKKβ phosphorylation in RAW264.7 cells. Similarly, the log phase KO infected RAW264.7 cells show dramatic increment of COX-2 expression and TNF-α secretion, compared to control or Ncb5or complement (CM) cell lines. The activation of inflammatory signaling pathways by KO mutant is significantly reduced when the RAW264.7 cells are pre-treated with BSA bound linoleate. Together, these findings confirmed that the leishmanial linoleate inhibits both COX-2 and TNF-α expression in macrophage via the inactivation of NF-κB signaling pathway. The stationary phase of KO promastigotes shows avirulence after infection in macrophages as well as inoculation into BALB/c mice; whereas CM cell lines show virulence. Collectively, these data provide strong evidence that de novo linoleate synthesis in Leishmania is an essential for parasite survival at extracellular promastigote stage as well as intracellular amastigote stage.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2024

Cytochrome b5 diversity in green lineages preceded the evolution of syringyl lignin biosynthesis

Abstract Lignin production marked a milestone in vascular plant evolution, and the emergence of syringyl (S) lignin is lineage specific. S-lignin biosynthesis in angiosperms, mediated by ferulate 5-hydroxylase (F5H, CYP84A1), has been considered a recent evolutionary event. F5H uniquely requires the cytochrome b5 protein CB5D as an obligatory redox partner for catalysis. However, it remains unclear how CB5D functionality originated and whether it coevolved with F5H. We reveal here the ancient evolution of CB5D-type function supporting F5H-catalyzed S-lignin biosynthesis. CB5D emerged in charophyte algae, the closest relatives of land plants, and is conserved and proliferated in embryophytes, especially in angiosperms, suggesting functional diversification of the CB5 family before terrestrialization. A sequence motif containing acidic amino residues in Helix 5 of the CB5 heme-binding domain contributes to the retention of CB5D function in land plants but not in algae. Notably, CB5s in the S-lignin–producing lycophyte Selaginella lack these residues, resulting in no CB5D-type function. An independently evolved S-lignin biosynthetic F5H (CYP788A1) in Selaginella relies on NADPH-dependent cytochrome P450 reductase as sole redox partner, distinct from angiosperms. These results suggest that angiosperm F5Hs coopted the ancient CB5D, forming a modern cytochrome P450 monooxygenase system for aromatic ring meta-hydroxylation, enabling the reemergence of S-lignin biosynthesis in angiosperms.

59 BASIC BIOLOGICAL SCIENCES↗
NASA NTRS1976

Line identifications in the ultraviolet spectra of Tau Herculis, B5 IV, and Zeta Draconis, B6 III

Tables of the lines found on two tracings each of the ultraviolet spectrum of Tau Her, B5 IV, and Zeta Dra, B6 III, made by the Copernicus satellite and possible identifications are given. The ranges 1025-1451A for Tau Her and 1035 to 1425A for Zeta Dra are covered by the U2 spectrometer at a resolution of 0.2A; the ranges 2028 to 2959A for Tau Her and 2000 to 3000A for Zeta Dra are covered by the V2 spectrometer at a resolution of 0.4A. The observed density of lines in the U2 region is 1.1 lines/A for Tau Her and 1.7 lines/A for Zeta Dra. In the V2 region it is 0.8 lines/A for Tau Her and 0.9 lines/A for Zeta Dra.

Underhill, A. B.↗
NASA NTRS1977

An abundance analysis of Tau Herculis, B5 IV

An abundance analysis of the sharp-lined star Tau Herculis (B5 IV) has been performed using a fully line-blanketed model atmosphere. The derived abundances are similar to those of the sun and the normal main-sequence B stars Iota Her (B3 V) and Nu Cap (B9 V).

Adelman, S. J.↗
NASA NTRS1992

Warm neutral halos around molecular clouds. IV - H I and continuum: Aperture synthesis observations towards the molecular cloud B5

We present aperture synthesis observations of H I (21 cm) line radiation and continuum emission at 408 and 1420 MHz towards a field centered on the molecular cloud B5. The H I emission shows an extended atomic halo around the molecular cloud. The opacity of the halo is derived using H I absorption toward several background sources and a simple source model is presented. The model indicates that the halo is not gravitationally bound to the molecular cloud and that it is in fact expanding away from it. Approximately 350 solar masses are contained in the H I halo. Flux densities and spectral indices for the sources detected in both of the continuum bands are given.

Andersson, B.-G.↗
NASA NTRS2015

NASA-DLR Collaboration Follow-Up: B5 - Surface Management

This presentation iintroduces a very high level overview of NASA aeronautics research and Airspace Operations and Safety Program (AOSP) under ARMD. It also provides high level overview of Airspace Technology Demonstration Project (ATD) under AOSP Program. Finally, suggestions and ideas for future collaboration with DLR on the Surface Management topic (B5) are presented for further discussion.

4D Taxi↗
DOE OSTI2020

Materials Data on B5(H2N)2 by Materials Project

BN2(BH)4 is Cubic alpha N2-derived structured and crystallizes in the monoclinic C2/c space group. The structure is zero-dimensional and consists of eight 13730-91-1 molecules and thirty-two boranediylradical molecules.

36 MATERIALS SCIENCE↗
NASA NTRS1991

Space dust and debris; Proceedings of the Topical Meeting of the Interdisciplinary Scientific Commission B (Meetings B2, B3, and B5) of the COSPAR 28th Plenary Meeting, The Hague, Netherlands, June 25-July 6, 1990

The present conference on space dust and debris encompasses orbital debris, in situ measurements and laboratory analysis of space-dust particles, comparative studies of comets, asteroids, and dust, the protection and maneuvering of spacecraft in space-debris environments, and the out-of-elliptic distribution of interplanetary dust derived from near-earth flux. Specific issues addressed include asteroid taxonomy, the optical properties of dust from cometary and interplanetary grains, light scattering by rough surfaces on asteroidal/lunar regoliths, and the first results of particulate impacts and foil perforations on the Long Duration Exposure Facility. Also addressed are collision probability and spacecraft disposition in the geostationary orbit, a flash on the moon caused by orbital debris, the limits of population growth in low earth orbit due to collisional cascading, and the simulation of cosmic man-made dust effects on space-vehicle elements in rocket and laboratory experiments.

Kessler, D. J.↗
NASA NTRS1996

The IRS 1 circumstellar disk, and the origin of the jet and CO outflow in B5

We report the discovery of the inner edge of the high velocity CO outflow associated with the bipolar jet originating from IRS 1 in Barnard 5 and the first ever resolution of its circumstellar disk in the 2.6 mm dust continuum and C18O. From high spatial resolution observations made with the Owens Valley Millimeter Array we are able to locate the origin of the outflow to within approximately 500 AU on either side of IRS 1 and apparently at the edge of, or possibly within, its circumstellar disk. The orientation of the continuum disk is perpendicular to the highly collimated jet outflow recently seen in optical emission at much farther distances. The disk has been detected in C18O giving a disk mass approximately 0.16 M (solar). Our HCO+ and HCN maps indicate significant chemical differentiation in the circumstellar region on small scales with HCO+ tracing an extended disk of material. The 12CO interferometer maps of the outflow show two conelike features originating at IRS 1, the blue one fanning open to the northeast and the red one to the southwest. The vertices of the cones are on either side of the circumstellar disk and have a projected opening angle of about 90 degrees. The intrinsic opening angle is in the range of 60 degrees-90 degrees which leads to significant interaction between outflow and infall.

NASA Center JPL↗
DOE OSTI2013

Dynamic Interaction Between Membrane-Bound Full-Length Cytochrome P450 and Cytochrome b 5 Observed by Solid-State NMR Spectroscopy

Microsomal monoxygenase enzymes of the cytochrome-P450 family are found in all biological kingdoms, and play a central role in the breakdown of metabolic as well as xenobiotic, toxic and 70% of the drugs in clinical use. Full-length cytochrome-b5 has been shown to be important for the catalytic activity of cytochrome-P450. Despite the significance in understanding the interactions between these two membrane-associated proteins, only limited high-resolution structural information on the full-length cytochrome-P450 and the cytochromes-b5-P450 complex is available. Here, we report a structural study on a functional ,72-kDa cytochromes-b5-P450 complex embedded in magnetically-aligned bicelles without having to freeze the sample. Functional and solid-state NMR (Nuclear Magnetic Resonance) data reveal interactions between the proteins in fluid lamellar phase bilayers. In addition, our data infer that the backbone structure and geometry of the transmembrane domain of cytochrome-b5 is not significantly altered due to its interaction with cytochrome-P450, whereas the mobility of cytochrome-b5 is considerably reduced.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
DOE OSTI2015

Characterization of deep level defects and thermally stimulated depolarization phenomena in La-doped TlInS{sub 2} layered semiconductor

Lanthanum-doped high quality TlInS{sub 2} (TlInS{sub 2}:La) ferroelectric-semiconductor was characterized by photo-induced current transient spectroscopy (PICTS). Different impurity centers are resolved and identified. Analyses of the experimental data were performed in order to determine the characteristic parameters of the extrinsic and intrinsic defects. The energies and capturing cross section of deep traps were obtained by using the heating rate method. The observed changes in the Thermally Stimulated Depolarization Currents (TSDC) near the phase transition points in TlInS{sub 2}:La ferroelectric-semiconductor are interpreted as a result of self-polarization of the crystal due to the internal electric field caused by charged defects. The TSDC spectra show the depolarization peaks, which are attributed to defects of dipolar origin. These peaks provide important information on the defect structure and localized energy states in TlInS{sub 2}:La. Thermal treatments of TlInS{sub 2}:La under an external electric field, which was applied at different temperatures, allowed us to identify a peak in TSDC which was originated from La-dopant. It was established that deep energy level trap BTE43, which are active at low temperature (T ≤ 156 K) and have activation energy 0.29 eV and the capture cross section 2.2 × 10{sup −14} cm{sup 2}, corresponds to the La dopant. According to the PICTS results, the deep level trap center B5 is activated in the temperature region of incommensurate (IC) phases of TlInS{sub 2}:La, having the giant static dielectric constant due to the structural disorders. From the PICTS simulation results for B5, native deep level trap having an activation energy of 0.3 eV and the capture cross section of 1.8 × 10{sup −16} cm{sup 2} were established. A substantial amount of residual space charges is trapped by the deep level localized energy states of B5 in IC-phase. While the external electric field is applied, permanent dipoles, which are originated from the charged B5 deep level defects, are aligned in the direction of the applied electric field and the equilibrium polarization can be reached in a relatively short time. When the polarization field is maintained, while cooling the temperature of sample to a sufficiently low degrees, the relaxation times of the aligned dipoles drastically increases. Practically, frozen internal electric field or electrets states remain inside the TlInS{sub 2}:La when the applied bias field is switched off. The influence of deep level defects on TSDC spectra of TlInS{sub 2}:La has been revealed for the first time.

75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND↗
NASA NTRS1974

The local complex of O and B stars. I - Distribution of stars and interstellar dust

The O-B5 stars, supergiants, young clusters, and associations within 1 kpc of the sun populate two flat systems inclined to each other by 19 to 22 deg. The historical background, statistical significance, composition, spatial arrangement of the contents, and interstellar extinction in the two belts are discussed. A more or less random distribution in space and in age characterizes the O-B5 stars of the 'galactic belt', which is aligned nearly along the Milky Way. The 'Gould belt' is inclined to the Milky Way (north in Sco-Oph and south in Orion), and exhibits a projected distribution of O-B5 stars in its mean plane that resembles a 'dragonfly', with five major features defining it. A crude 'diameter' of the system is 750 to 1000 pc, and the sun's position is eccentric, lying toward Ophiuchus. The nuclear age of the system, while not unique, may be characterized as 30 m.y. from the spectral type of the broad main-sequence turnup near B2-5. Most of the O-B2 stars and youngest stellar groups near the sun belong to the Gould belt, but both belts have approximately equal space densities of B3-B5 stars and similar average values of interstellar extinction.-

Stothers, R.↗