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Two proposed methods for grading beef quality based on inspection by electronic equipment: one method uses television camera to generate image of a cut of beef as customer sees it; other uses ultrasonics to inspect live animal or unsliced carcasses. Both methods show promise for automated meat inspection.
As part of the U.S. EPA Dioxin Reassessment Program, the 2,3,7,8-chlorine-substituted dibenzo-p-dioxins and furans were measured at part per trillion (ppt) levels in beef fat collected from slaughter facilities in the United States. This is the first statistically designed national survey of these compounds in the U.S. beef supply. Analyte concentrations were determined by high-resolution gas chromatography/high-resolution mass spectrometry, using isotope dilution methodology. Method limits of detection on a whole weight basis were 0.05 ppt for TCDD and 0.10 ppt for TCDF, 0.50 ppt for the pentas (PeCDDs/PeCDFs)/hexas (HxCDDs/HxCDFs)/heptas (HpCDDs/HpCDFs), and 3.00 ppt for the octas (OCDD/OCDF). Method detection and quantitation limits were established on the basis of demonstrated performance criteria utilizing fortified samples rather than by conventional signal-to-noise or variability of response methods. The background subtraction procedures developed for this study minimized the likelihood of false positives and increased the confidence associated with reported values near the detection limits. Mean and median values for each of the 2,3,7,8-Cl-substituted dioxins and furans are reported, along with the supporting information required for their interpretation. The mean toxic equivalence values for the samples are 0.35 ppt (nondetects = 0) and 0.89 ppt (nondetects = 1/2 LOD).
Isolation, crystallization, and physicochemical properties of beef and chicken enzymes - lactic dehydrogenases
Reflections in ultrasonic A-scan signatures of beef carcasses indicate USDA grade. Since reflections from within muscle are determined primarily by fat/muscle interface, richness of signals is direct indication of degree of marbling and quality. Method replaces subjective sight and feel tests by individual graders and is applicable to grade analysis of live cattle.
The results of a study conducted to assist the Department of Agriculture in the task of considering innovative methods for the grading of carcass beef for human consumption is presented. The processing of photographic, television and ultrasound images of the longissimus dorsi muscle at the 12/13th rib cut was undertaken. The results showed that a correlation could be developed between the quality grade of the carcass as determined by a professional grader, and the fat to area ratio of the muscle as determined by image processing techniques. In addition, the use of ultrasound shows the potential for grading of an unsliced carcass or a live animal.
Only rare quartz grains with single-set planar (1013) deformation features (PDF's) are present in breccia dikes found in association with uniformly oriented shatter cones that occur over an area 8 x 25 km. This suggests that the Beaverhead shocked rocks come from only the outer part of the central uplift of what must have been a large (greater than 100 km diameter) complex impact structure. An impact event of this magnitude on continental crust (thought to have occurred in late Precambrian or ealy Paleozoic time) could be expected to punctuate local geologic history. Furthermore, although it may now be covered, its scar should remain despite all the considerable subsequent erosion/deposition and tectonism since the impact. The following are three large-scale singularities or anomalies that may reflect the event and mark its source. (1) The Lemhi Arch is a major structural uplift that occurred in late Proterozoic-early Paleozoic time in East Central Idaho and caused the erosion of at least 4 km of sedimentary cover. This may be directly related to the impact. (2) Of the many thrust sheets comprising the Cordilleran belt, the Cabin plate that carries the shocked rocks is unique in that it alone intersected the crystalline basement. It also now marks the apex of the Southwest Montana Recess in the Sevier front. The basement uplift remaining from the impact may have constituted a mechanical obstacle to the advancing thrust sheets in Cretaceous time, causing the recess. (3) What could be interpreted as a roughly circular aeromagnetic anomaly approx. 70 km in diameter can be discerned in the state aeromagnetic map centered about 20 km southeast of Challis, Idaho, in the Lost River range. It is in approximately the right place, and ignoring the possibility that the anomalies have diverse causes and the circular pattern is coincidental, it may mark what remains of the buried central uplift structure.
The Science and Technology Lab (STL) is tasked with the design, development, and application of the science and engineering services. Formed in the early 1970's STL adhered to many traditional attitudes including barriers to communication, excessive management control, parochial strategies, unclear measures of success, lack of customer focus, underutilization of people, and excessive administrative burdens on scientists and engineers. The challenge for the STL was to maximize customer satisfaction through the effective and efficient application of the notable skills and talents of the STL's workforce. In this way, the Lab would begin its exciting journey toward becoming world class. A discussion of this on-going transformation is presented.
This presentation discusses various misgivings concerning the directions and productivity of Distributed Parameter System (DPS) theory as applied to spacecraft vibration control. We try to show the need for greater cross-fertilization between DPS theorists and spacecraft control designers. We recommend a shift in research directions toward exploration of asymptotic frequency response characteristics of critical importance to control designers.
Rapid, direct methods are needed to assess active bacterial populations in water and foods. Our objective was to determine the efficiency of bacterial detection by immunomagnetic separation (IMS) and the compatibility of IMS with cyanoditolyl tetrazolium chloride (CTC) incubation to determine respiratory activity, using the pathogen Escherichia coli O157:H7. Counterstaining with a specific fluorescein-conjugated anti-O157 antibody (FAb) following CTC incubation was used to allow confirmation and visualization of bacteria by epifluorescence microscopy. Broth-grown E. coli O157:H7 was used to inoculate fresh ground beef (<17% fat), sterile 0.1% peptone, or water. Inoculated meat was diluted and homogenized in a stomacher and then incubated with paramagnetic beads coated with anti-O157 specific antibody. After IMS, cells with magnetic beads attached were stained with CTC and then an anti-O157 antibody-fluorescein isothiocyanate conjugate and filtered for microscopic enumeration or solid-phase laser cytometry. Enumeration by laser scanning permitted detection of ca. 10 CFU/g of ground beef or <10 CFU/ml of liquid sample. With inoculated meat, the regression results for log-transformed respiring FAb-positive counts of cells recovered on beads versus sorbitol-negative plate counts in the inoculum were as follows: intercept = 1.06, slope = 0.89, and r2 = 0. 95 (n = 13). The corresponding results for inoculated peptone were as follows: intercept = 0.67, slope = 0.88, and r2 = 0.98 (n = 24). Recovery of target bacteria on beads by the IMS-CTC-FAb method, compared with recovery by sorbitol MacConkey agar plating, yielded greater numbers (beef, 6.0 times; peptone, 3.0 times; water, 2.4 times). Thus, within 5 to 7 h, the IMS-CTC-FAb method detected greater numbers of E. coli O157 cells than were detected by plating. The results show that the IMS-CTC-FAb technique with enumeration by either fluorescence microscopy or solid-phase laser scanning cytometry gave results that compared favorably with plating following IMS.
A new commercial product that can preserve perishable commodities for weeks without freezing, so that they can be shipped fresh without the cost of air freight, has been developed by Grumman Corporation, Bethpage, Long Island, New York. The development benefited from the company's experience in developing the environmental control system for the Lunar Module, which delivered Apollo astronauts to the surface of the moon. Called Dormavac, the system provides a commodity-preserving environment within an aluminum container that can be transported by truck, rail or ship. Dormavac creates a cold-but above freezing-environment with high relative humidity and very low air pressure. The saturated air minimizes commodity weight loss and the air is automatically changed several times an hour to flush away odors and harmful gases released by the commodities. According to company literature, Dormavac significantly extends the transportation life of perishables. For example, pork has a normal cold storage life of about seven days, beef two weeks and tomatoes three weeks; with Dormavac, pork remains fresh for three weeks, beef more than six weeks and tomatoes seven weeks or more. Dormavac is manufactured and marketed by Grumman Allied Industries, Woodbury, New York. In developing the system, Grumman Allied drew upon the technological resources of another company subsidiary, Grumman Aerospace. Engineers who had earlier worked on Lunar Module environmental control brought their know-how and experience to the Dormavac development.
This article implements a spatially explicit model to estimate the probability of forest and agricultural fires in the Brazilian Amazon. We innovate by using variables that reflect farmgate prices of beef and soy, and also provide a conceptual model of managed and unmanaged fires in order to simulate the impact of road paving, cattle exports, and conservation area designation on the occurrence of fire. Our analysis shows that fire is positively correlated with the price of beef and soy, and that the creation of new conservation units may offset the negative environmental impacts caused by the increasing number of fire events associated with early stages of frontier development.
Refutation of Sylven-Snellman report of catalysis of benzoylarginine beta-naphthylamide and leucine beta-naphthylamide hydrolysis by beef spleen cathespin B
Description of the properties of a carboxypeptidase, termed 'catheptic carboxypeptidase C,' and a dipeptidase, termed 'Ser-Met dipeptidase.' Both were found in highly purified DAP I from either beef spleen or rat liver. Methods are described for the removal or selective inactivation of these contaminating enzymes.
A study and experimental investigation has been performed to determine the feasibility of measuring regional blood flow and volume in man by means of microwave radiometry. An indication was expected of regional blood flow from measurement of surface and subsurface temperatures with a sensitive radiometer. Following theoretical modeling of biological tissue, to determine the optimum operating frequency for adequate sensing depth, a sensitive microwave radiometer was designed for operation at 793 MHz. A temperature sensitivity of of 0.06 K rms was realized in this equipment. Measurements performed on phantom tissue models, consisting of beef fat and lean beefsteak showed that the radiometer was capable of sensing temperatures from a depth between 3.8 and 5.1 cm. Radiometric and thermodynamic temperature measurements were also performed on the hind thighs of large dogs. These showed that the radiometer could sense subsurface temperatures from a depth of, at least, 1.3 cm. Delays caused by externally-generated RF interference, coupled with the lack of reliable blood flow measurement equipment, prevented correlation of radiometer readings with reginal blood flow. For the same reasons, it was not possible to extend the radiometric observations to human subjects.
The author has identified the following significant results. Emphasis has been given to an inventory of land resource types and land use at the ten Great Plains Corridor test sites. A resource and land use classification system was developed which uses available soil survey information and interpretations from NASA obtained high flight aerial photography to locate discrete areas of similar rangeland vegetation. Existing classification systems, even those developed for use with remote sensor data, were found to be inadequate for this project. This system is expected to be of general use for remote sensing related to land use and management. It has specific applicability to any effort aimed at regional use of ERTS-1 MSS digital data products. A preliminary assessment of the relative importance of rangelands in the Great Plains Corridor states indicates that the value of the livestock industry supported by this resource exceeds 23 billion dollars. The development of a Rangeland Feed Conditions index for this region could be used by more than 400,000 farm and ranch operators involved in the production of more than 40% of the nation's beef and much of the country's grain.
The author has identified the following significant results. A pilot study was conducted to determine the feasibility of mapping land use in the Great Lakes Basin area utilizing ERTS-1 data. Small streams were clearly defined by the presence of trees along their length in predominantly agricultural country. Field patterns were easily differentiated from forested areas; dairy and beef farms were differentiated from other farmlands, but no attempt was made to identify crops. Large railroad lines and major highway systems were identified. The city of Erie and several smaller towns were identified, as well as residential areas between these towns, and docks along the shoreline in Erie. Marshes, forests, and beaches within Presque Isle State Park were correctly identified, using the DCLUS program. Bay water was differentiated from lake water, with a small amount of misclassification.
Six healthy male volunteers underwent two 1-week periods of bedrest, each preceded and followed by 2-week control and recovery periods. The daily metabolic diet contained 150 mEq of sodium. Following one 7-day bedrest period, each man was subjected to LBNP at a level of -30 mm Hg for 4 hr while consuming 1000 ml of beef bouillon containing 154 mEq of sodium. After the other bedrest period, each man simply consumed the bouillon without LBNP treatment during 4 hr of continued bedrest. Measurements of plasma volume and orthostatic tolerance were made before and after each treatment period. After combined LBNP and saline therapy, plasma volume and response to LBNP testing showed a return to pre-bedrest levels. Saline consumption alone had a lesser effect. With continuation of bedrest in three subjects, the beneficial effects of these measures appeared to be largely gone after 18 hr.
A conceptual design was developed by Northrop/LTV for an advanced C-130 Center Wing Box (CWB) which could meet the severe mission requirements of the SOF C-130 aircraft. The goals for the advanced technology CWB relative to the current C-130H CWB were: (1) the same acquisition cost; (2) lower operating support costs; (3) equal or lower weight; (4) a 30,000 hour service life for the SOF mission; and (5) minimum impact on the current maintenance concept. Initially, the structural arrangement, weight, external and internal loads, fatigue spectrum, flutter envelope and design criteria for the SOF C-130 aircraft CWB were developed. An advanced materials assessment was then conducted to determine the suitability of advanced materials for a 1994 production availability and detailed trade studies were performed on candidate CWB conceptual designs. Finally, a life-cycle cost analysis was performed on the advanced CWB. The study results showed that a hybrid composite/metallic CWB could meet the severe SOF design requirements, reduce the CWB weight by 14 pct., and was cost effective relative to an all metal beefed up C-130H CWB.