Electron Spin Resonance (ESR) for the study of Reactive Oxygen Species (ROS) on the isolated frog skin (Pelophylax bergeri): A non-invasive method for environmental monitoring
Highlights: • ROS levels in frog skin biopsy were determined by spin-trapping ESR spectroscopy. • Skin of frogs collected in polluted areas contains higher ROS concentration. • ROS levels determined in skin biopsy follow the same trend of those from liver samples. • Immunohistochemical analyses and enzymatic activity tests validate the method. • ROS concentration determined by ESR in biosentinel biopsy is an effective biomarker. Reactive oxygen species (ROS) in biological tissues of elected biosentinels represent an optimal biomarker for eco-monitoring of polluted areas. Electron spin resonance (ESR) is the most definitive method for detecting, quantifying and possibly identifying radicals in complex systems.
54 ENVIRONMENTAL SCIENCES↗